From 17f03fa34f3b0f12948dd464486824121523d09f Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Miguel=20Juli=C3=A1=20Molina?= Date: Sat, 23 May 2020 11:07:28 +0200 Subject: [PATCH 01/95] Added 3D printable solution for magdeck V1 --- .../OT2 Magedck V1 Elevator Frame.stl | Bin 0 -> 87684 bytes .../OT2_MagdeckV1_ElevatorFrame_3D.JPG | Bin 0 -> 112123 bytes .../OT2_MagedckV1_ElevatorFrame_template.jpg | Bin 0 -> 615057 bytes .../MagdeckV1_ElevatorFrame/Readme.md | 0 4 files changed, 0 insertions(+), 0 deletions(-) create mode 100644 ot2-modifications/MagdeckV1_ElevatorFrame/OT2 Magedck V1 Elevator Frame.stl create mode 100644 ot2-modifications/MagdeckV1_ElevatorFrame/OT2_MagdeckV1_ElevatorFrame_3D.JPG create mode 100644 ot2-modifications/MagdeckV1_ElevatorFrame/OT2_MagedckV1_ElevatorFrame_template.jpg create mode 100644 ot2-modifications/MagdeckV1_ElevatorFrame/Readme.md diff --git a/ot2-modifications/MagdeckV1_ElevatorFrame/OT2 Magedck V1 Elevator Frame.stl 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z#E0`0TJ<6~mZ;k(pjOH|N($;$Q#`&%MpI;0 z?xdT;07n^284oJ5*}R0T7hijD(bI=gN0~kt}l#f-UG&Bd(n4PIZ}a@kci0C z9q@*4*+{{L5eMvbQE%J4p+|;!KjMX`%T1ZLLOX+Qa~j1YB6 zh+Z_7_GhW}ydgITDa!vqSX29@uyW_3L3E9eF1MTq4R-dz_Fe=NdXXcRLJV9L|EL=O zK_E4L8FSMQ0|$F=?2W5)dE6 zPX}*Udl`T1T~m)#o^-n*X^8RtyEy(HAI7@Yhe~KRgenN!`B3l= z9&?e?S&l;p>TlEo7tX67DTt00`MxGM8@__ItCC;n|-q@o@tT7s<<*JhwkDi(L5+v zeEY{@>D0G;^J6^i92~4&=U=}^o79G8=Ps{t8ZrGqceRS)n(3w_sDbyKI^TB)nHw< zu5gN2f#1Gaj~(bf_}q^}N@3DWj;KxKcE6^~#3dw}65bwXM+59)REVGtd;2Puam#sPHl~2y`+inH6GoW$UaaV&UQ3^LlLroVOdqV~} z-kuc85QXN%!JJ}dcdJU{TbKHol%TskkWCw}jy&39u2)s8K@fb>8C{t30)`!eaNR^YHB84V5?nj1&l?MwU?@yg`SEQT1!M~$=z@@Ee^vUqUZk8u+6Z;8P zXf)g&Z$#8S13Ms^%)U^zZoD+1su!6q##K%)k90456WQ*0+t?#n|2JpPldEYv>S@1# zqf%ioXP=|TjNtnB-pRubxyCzB8JzvcoRz(eviv~QRUxcOYIP6F%gp-Z+UtcM5LBZn zi|E;ld})hnlA?J!P?gP@!rT(ti%$NlXa%M=?QB?MBc8scv*7_eOc2=Zvw|nX(3ywn z5!ot^RGUy1q+Pr^50d*vb8kiZW>d>-c1Hmj0M!PHU?iV7(K4;Zb0&CuC+?+CmTfU8pdvD)>0zzD1XQb>O3&A zhHwXO8F^~wE%ja|LQKb3E(Oc3>Rk{V@}pz-s@-l+iXN8BO1aWd^sO)XC}UmeR=k3q z)?hz<}6kQBacW_M(oW zY%gfRDg0YdL$id~R}O1!Socq;I=Ny813!?t76Y^1TIJ}^=Brk1&bQMRCB_UtX;gx9 zIm0R Date: Sat, 23 May 2020 11:10:22 +0200 Subject: [PATCH 02/95] Redirected images in devel branch documents to devel branch folders --- doc/S3/03_ethernet_connection.md | 4 +- manuals/manual_eng.md | 58 +++++++++---------- manuals/manual_esp.md | 58 +++++++++---------- .../MagdeckV1_ElevatorFrame/Readme.md | 15 +++++ 4 files changed, 75 insertions(+), 60 deletions(-) diff --git a/doc/S3/03_ethernet_connection.md b/doc/S3/03_ethernet_connection.md index 499cae5..8eb9690 100644 --- a/doc/S3/03_ethernet_connection.md +++ b/doc/S3/03_ethernet_connection.md @@ -21,7 +21,7 @@ dns-search= method=auto ``` -This configuration will prepare your robots to get a dynamic IP address that must be set to fixed one by reserving it in your network DHCP server. If you wanted to set a manual address, change the `[ipv4]` field as we did in the [wifi setup file](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/doc/S3/01_OT2_installation.md): +This configuration will prepare your robots to get a dynamic IP address that must be set to fixed one by reserving it in your network DHCP server. If you wanted to set a manual address, change the `[ipv4]` field as we did in the [wifi setup file](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/doc/S3/01_OT2_installation.md): ``` [ipv4] @@ -35,7 +35,7 @@ method=manual 3) The easiest way to tidy up the cable without drilling or cutting is by passing it through the left corner of the plastic cover, where there is already an aperture to pass the other cables. Then, take the cable down using the magnetic cable management hooks for the USB cables of the modules and take it out of the box through the bottom left hole of the rear panel. -![Ethernet cable configuration](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/ethernet_cable_configuration.jpg?raw=true) +![Ethernet cable configuration](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/ethernet_cable_configuration.jpg?raw=true) 4) Plug the cable and restart the robot. Now you should be able to find the robot in your wired network. Use the DHCP server to reserve a fix IP for the robot IPs. diff --git a/manuals/manual_eng.md b/manuals/manual_eng.md index 7b5dca2..60aba47 100644 --- a/manuals/manual_eng.md +++ b/manuals/manual_eng.md @@ -14,11 +14,11 @@ The robots are distributed in 2 different locations: - **Extraction Room**: Located in Orientación Diagnóstica, here are robots **A1, A2, B1, B2, B3 and B4** in the same room with a PC to control them. -![extraction_room_setup.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/extraction_room_setup.jpg?raw=true) +![extraction_room_setup.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/extraction_room_setup.jpg?raw=true) - **PCR prep Room**: Inside Virus Respiratorios we find robots **C1 y C2** paired with another PC for their control. -![pcrprep_room_setup.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/pcrprep_room_setup.jpg?raw=true) +![pcrprep_room_setup.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/pcrprep_room_setup.jpg?raw=true) ## Powering on the robots @@ -26,18 +26,18 @@ Before starting the robot, please make sure there are no tips attached to any pi To power on the robot, press the power button located at the rear of the left lateral panel, just above where the power cable is plugged. -![robot_power_button.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/robot_power_button.jpg?raw=true) +![robot_power_button.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/robot_power_button.jpg?raw=true) The robot will produce some mechanical noises and it si possible the arem moves home several times. The front led button will blink blue during the startup process. Once the noise is over and the arm stops moving, the front led will glow blue permanently signalling the robot is ready to take orders. -![robot_front_led.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/robot_front_led.jpg?raw=true) +![robot_front_led.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/robot_front_led.jpg?raw=true) Finally, for robot configurations **B** and **C** we will have to power on the magdeck and tempdeck, respectively. Press the power button at the rear of each module, next to wher its power cord is plugged. A white light will glow and once the module stops making noises it will be ready to use. -![tempdeck_power_button.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/tempdeck_power_button.jpg?raw=true) -![magdeck_power_button.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/magdeck_power_button.jpg?raw=true) +![tempdeck_power_button.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/tempdeck_power_button.jpg?raw=true) +![magdeck_power_button.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/magdeck_power_button.jpg?raw=true) **Note**: The unit **A2** has no blue led in the front button, so it will not show that the robot is booting up neither it is ready to be used. As a consecuence, we will need to check its status through the app as we will explain later. @@ -45,8 +45,8 @@ Finally, for robot configurations **B** and **C** we will have to power on the m **It smells like something is burning or I see smoke**: Quickly power off the robot or module where the smoke comes from by pushing the power button at the rear of the left side panel. Check that electric plugs are correctly plugged matching the marks inside the male conector with the marks in the female one. If one of the pins has become black or everything was already correctly connected, do not power off the robot and contact support. -![power_connector_frontal_male.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/power_connector_frontal_male.jpg?raw=true) -![power_connector_frontal_female.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/power_connector_frontal_female.jpg?raw=true) +![power_connector_frontal_male.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/power_connector_frontal_male.jpg?raw=true) +![power_connector_frontal_female.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/power_connector_frontal_female.jpg?raw=true) **I forgot to remove the tip from the pippete before powering on and ...**: Quickly power off the robot by pushing the power button at the rear of the left side panel. Once it has stopped, clean any liquid that can have been split and contact support to evaluate potential damage or discalibration caused by the colition. @@ -58,29 +58,29 @@ The PCs located in the romms of the robots have a common user logging which gran Once you are logged in, look for the icon of Opentrons app and open it. -![opentrons_app_icon.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_app_icon.jpg?raw=true) +![opentrons_app_icon.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_app_icon.jpg?raw=true) The app starts in developper mode, which provides some extra features we need for the correct execution of our protocols, but makes the interface a less user-friendly. Close the debug mode of the app and we are ready to go on. Close any update mesagges, too. Each update needs to be tested before installing, as they will also update the robots and can modify their behaviour. -![close_debug_mode.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/close_debug_mode.jpg?raw=true) +![close_debug_mode.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/close_debug_mode.jpg?raw=true) The app interface is divided in 3 vertical sections: -![opentrons_app_mainwindow.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_app_mainwindow.jpg?raw=true) +![opentrons_app_mainwindow.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_app_mainwindow.jpg?raw=true) - Left you have a menu to navigate between the windows robot, protocol, calibration and run. -![opentrons_app_leftmenu.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_app_leftmenu.jpg?raw=true) +![opentrons_app_leftmenu.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_app_leftmenu.jpg?raw=true) - In the middle panel we have the orders we can execute in the robot from the active window. -![opentrons_app_middlemenu.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_app_middlemenu.jpg?raw=true) +![opentrons_app_middlemenu.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_app_middlemenu.jpg?raw=true) - To the right we have the largest panel, which shows information for the user and different configuration and interative options. As the previous panel, it contents depen on the active window. -![opentrons_app_rightmenu.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_app_rightmenu.jpg?raw=true) +![opentrons_app_rightmenu.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_app_rightmenu.jpg?raw=true) ## Connecting to a robot @@ -90,15 +90,15 @@ Click on the first option displayed in the left menu inside the app: `Robot`. Th **Note**: Type **C** robots does not have lights. -![opentrons_app_mainrobot.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_app_mainrobot.jpg?raw=true) +![opentrons_app_mainrobot.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_app_mainrobot.jpg?raw=true) In order to operate a robot, we need to connect to it. You can do it either by activating the little slider button at the right of its name in the middle panel or by clicking on the `Connect``button in the right panel after selecting the robot in the middle one. - ![opentrons_app_connecttorobot.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_app_connecttorobot.jpg?raw=true) + ![opentrons_app_connecttorobot.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_app_connecttorobot.jpg?raw=true) A green banner at the top will confirm you are sucessfully connecte to a robot and its name. - ![opentrons_app_connectedrobot.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_app_connectedrobot.jpg?raw=true) + ![opentrons_app_connectedrobot.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_app_connectedrobot.jpg?raw=true) **Note**: You can only be connected to one robot at the same time. All the robots will continue executing their given orders after disconnected, but any configuration done in a robot might get lost if disconnected before starting the run, so it is recommended that in case of disconnetion you start configurating your experiment again. @@ -110,7 +110,7 @@ In order to operate a robot, we need to connect to it. You can do it either by a Click on the second option of the left menu: `Protocol`. In this window you can see which protocol is already loaded in the robot from the previous run in the right panel. -![opentrons_app_mainprotocol.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_app_mainprotocol.jpg?raw=true) +![opentrons_app_mainprotocol.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_app_mainprotocol.jpg?raw=true) To load a new protocol, drag and drop the corresponding `.py` file in the middle panel or click on the button `Open` in the same panel and look for the desired file. @@ -128,7 +128,7 @@ In the right panel we can check tne name of the loaded protocol, version, author The third option of the menu on the left, `Calibrate`, allows us to adjust the arm position and its movement inside the hood so the robot can do it preciselly and match the labware. -![opentrons_app_maincalibrate.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_app_maincalibrate.jpg?raw=true) +![opentrons_app_maincalibrate.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_app_maincalibrate.jpg?raw=true) **Protocol calibration is only needed when:** - it is the first time this protocol is being executed in this robot @@ -147,7 +147,7 @@ Calibration must be started with an ampty hood (but modules, as they have to rem Calibration will start with the pipettes. It will guide you step by step to remove the trash for the tips and attach a new tip to the pipettes, and the arm will move to touch the sensors usually coveretd by the trash in order to check the movement and poitioingn of the tips. Once both pipettes have been calibrated, carefully put back the trash in its place to cever the sensors. -![opentrons_calibration_metalpins.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_calibration_metalpins.jpg?raw=true) +![opentrons_calibration_metalpins.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_calibration_metalpins.jpg?raw=true) **Note**: Sensors are tiny delicate metal pins. Be very careful when removing or setting the trash, or they could be damaged. @@ -159,11 +159,11 @@ Calibration will start with the pipettes. It will guide you step by step to remo Once pipette calibration is complete and you start calibrating the first labware, the tipracks, the app will show a layout of how to set up modules (if they are required) and labware inside the hood. Follow the instructions to set up the labware as shown, but do not put liquids yet, only empty labware. Follow the instructions of the section `Colocar el labware en la cabina` in this manual to do it right. For the moment, we are only calibrating and liquids would become a hazard, potentially splitting and contaminating both labware and deck. -![opentrons_calibration_labwarelayout.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_calibration_labwarelayout.jpg?raw=true) +![opentrons_calibration_labwarelayout.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_calibration_labwarelayout.jpg?raw=true) Now lets move to calibrate tipracks. The robot will move the corresponding pipette above the top-left tip of each tiprack,and using the contols in the app you must move the lower end of the pipette to the height of the top end of the tip and centered into it. When it is in the right position, it will try to attach the tip and if it achives it the tiprack will be calibrated. If it fails to do it, repeat the process until it is done correctly. -![opentrons_calibration_controls.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_calibration_controls.jpg?raw=true) +![opentrons_calibration_controls.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_calibration_controls.jpg?raw=true) **Note**: The 1000µl pipette can shake several times when picking up a new tip. This behaviour does not depend on the robot but the app, and can be disabled inside the app in the window `Robot`, in the section `PIPETTES & MODULES` of the middle panel. Click on `SETTINGS` next to the pipette and there is a checkbox saying `shaking` that can be disabled. @@ -185,11 +185,11 @@ In order to set up the labware into the hood, follow the instructions provided b - If possible, put the tubes and boxes with their lid on, and open them only before starting the run. - When building an Opentrons' rack, make sure the marked corner of the stand matches with the marked corner of the rack. That marked corner will go on the top-left of the deck slot. -![opentrons_labware_opentronsrack.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_labware_opentronsrack.jpg?raw=true) +![opentrons_labware_opentronsrack.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_labware_opentronsrack.jpg?raw=true) - When putting a piece of labware in a deck slot, always put first the bottom-right corner into the metal hooks. Then the piece should easily fall into position. -![opentrons_labware_deckslot.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_labware_deckslot.jpg?raw=true) +![opentrons_labware_deckslot.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_labware_deckslot.jpg?raw=true) - Make sure the tubes are in the right position in their racks. - Set up all the tip boxes as if the protocol was going to be executed for the maximum number of samples and steps. @@ -202,7 +202,7 @@ The last option of the left panel menu, `Run`, allow us to give the execution or This order starts the tun of the uploaded protocol. Make sure that the loaded protocol is the right one, that you are connected to the correct robot, that the labware is setup and without lids on, that reuired modules are on and pipettes with no tips attached before clicking on the button. - ![opentrons_run_start.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_run_start.jpg?raw=true) + ![opentrons_run_start.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_run_start.jpg?raw=true) - `PAUSE`: @@ -210,13 +210,13 @@ This order starts the tun of the uploaded protocol. Make sure that the loaded pr **Note**: In case the robot finds a recuperable execution error, like door is open, tips are over or trash is full, the execution will pause as if the button `PAUSE` was cliked on. This status is notified to the user both verbally and via text in the log. Once the problem is fixed, click on `RESUME` to resume the run. - ![opentrons_run_pause.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_run_pause.jpg?raw=true) + ![opentrons_run_pause.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_run_pause.jpg?raw=true) - `RESUME`: Resume execution after pausing. - ![opentrons_run_pauserestart.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_run_pauserestart.jpg?raw=true) + ![opentrons_run_pauserestart.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_run_pauserestart.jpg?raw=true) - `CANCEL RUN`: @@ -224,13 +224,13 @@ Abort run in progress. This order requires confirmation and all progress will be **Note**: After cancelling a run, the robot will erase all information about the run, so it is reommended to refill al tips before running anything again. -![opentrons_run_cancelrunconfirmation.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_run_cancelrunconfirmation.jpg?raw=true) +![opentrons_run_cancelrunconfirmation.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_run_cancelrunconfirmation.jpg?raw=true) - `RESET RUN`: Afeter finishing a run the robot will stop. The top baner colour and message will show if the run ended successfully (green), if it is paused (yellow) or got aborted or critical error (red). In order to run the same protocol again it is not needed to upload the same protocol file, but just click on this button and the robot will prepare everything for a new run using the same protocol file. -![opentrons_run_resetrun.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_run_resetrun.jpg?raw=true) +![opentrons_run_resetrun.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_run_resetrun.jpg?raw=true) A log of the process will be displayed at all times in the right panel of this window. While running, the log will scroll down and marking in blue the current step , in grey the already completed steps and in black the remaining ones. diff --git a/manuals/manual_esp.md b/manuals/manual_esp.md index 78bd4cb..17d6fc2 100644 --- a/manuals/manual_esp.md +++ b/manuals/manual_esp.md @@ -14,11 +14,11 @@ Los robots se encuentran localizados en 2 laboratorios diferentes, distinguiendo - **Sala de extracción**: Situada en Orientación Diagnóstica, encontramos en una misma sala los robots **A1, A2, B1, B2, B3 y B4** junto con un PC para su operación. -![extraction_room_setup.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/extraction_room_setup.jpg?raw=true) +![extraction_room_setup.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/extraction_room_setup.jpg?raw=true) - **Sala de preparación de PCR**: En Virus Respiratorios se encuentran lo robots **C1 y C2** junto a un otro PC para su operación. -![pcrprep_room_setup.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/pcrprep_room_setup.jpg?raw=true) +![pcrprep_room_setup.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/pcrprep_room_setup.jpg?raw=true) ## Encender los robots @@ -26,18 +26,18 @@ Antes de enceder el robot, asegúrate de que no hay puntas en las pipetas ni obj Para encender los robots, utiliza el botón de encendido que se encuentra al fondo del panel lateral izquierdo, justo sobre la toma de corriente del robot. -![robot_power_button.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/robot_power_button.jpg?raw=true) +![robot_power_button.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/robot_power_button.jpg?raw=true) El robot emitirá unos sonidos de engranajes y es posible que el brazo se mueva hasta la posición de inicio unas cuantas veces. El botón frontal del aparato parpadeará en color azul durante el proceso. Una vez se paren los ruidos y el brazo del robot esté detenido, el led del botón frontal pasará a brillar en color azul de forma fija indicando que el robot está listo para ser utilizado. -![robot_front_led.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/robot_front_led.jpg?raw=true) +![robot_front_led.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/robot_front_led.jpg?raw=true) Finalmente, en el caso de los robots tipo **B** y **C** tendremos que encender los módulos magnético y de temperatura respectivamente. Para ello, presiona le botón de encendido de cada módulo situado en la parte trasera del mismo. Cuando la luz trasera se encienda y el módulo deje de emitir sonidos estará listo para usarse. -![tempdeck_power_button.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/tempdeck_power_button.jpg?raw=true) -![magdeck_power_button.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/magdeck_power_button.jpg?raw=true) +![tempdeck_power_button.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/tempdeck_power_button.jpg?raw=true) +![magdeck_power_button.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/magdeck_power_button.jpg?raw=true) **Nota**: En el robot **A2** el led del botón frontal no funciona en color azul, por lo que no nos indicará que está en proceso de arranque con parpadeos ni que está listo para su uso brillando fijo en color azul, deberemos comprobarlo a través de la aplicación como explicaremos más adelante. @@ -45,8 +45,8 @@ Finalmente, en el caso de los robots tipo **B** y **C** tendremos que encender l **Huele a quemado o veo humo**: Apaga rápidamente el robot o módulo del que procede el olor/humo pulsando rápidamente el botón de apagado del robot al fondo del panel lateral izquierdo. Comprueba que los conectores de corriente están correctamente conectados haciendo coincidir la parte plana del conector macho con la parte plana del hembra y lo mismo con la muesca interna. Si observas que alguno de los pines se ha vuelto negro o estaba bien conectado, no vuelvas a encender el robot y llama a soporte. -![power_connector_frontal_male.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/power_connector_frontal_male.jpg?raw=true) -![power_connector_frontal_female.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/power_connector_frontal_female.jpg?raw=true) +![power_connector_frontal_male.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/power_connector_frontal_male.jpg?raw=true) +![power_connector_frontal_female.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/power_connector_frontal_female.jpg?raw=true) **Se me ha olvidado quitar la punta de la pipeta antes de encenderlo y ...**: Apaga el robot de nuevo pulsando rápidamente el botón de apagado del robot al fondo del panel lateral izquierdo. Una vez se haya detenido, recoge cualquier líquido que pueda haberse derramado y pide soperte para poder evaluar posibles daños o desajustes producisdos por las colisiones. @@ -58,29 +58,29 @@ Los PCs instalados en las mismas salas que los robots tienen un usuario común q Una vez en el escritorio del equipo, busca el logo de la aplicación Opentrons y ábrela. -![opentrons_app_icon.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_app_icon.jpg?raw=true) +![opentrons_app_icon.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_app_icon.jpg?raw=true) La aplicación se abre en modo desarrollador, ya que de otra forma no tendríamos acceso a algunas características necesarias para el correcto funcionamiento de nustros robots, pero a cambio produce efectos en la interfaz que la pueden hacer menos amigable. Cierra el modo debug de la aplicación y podemos continuar. Cierra también las peticiones de actualización. Cada actualización debe ser testada a fondo antes de ser instalada, ya que también actualizará los robots y puede modificar su funcionamiento. -![close_debug_mode.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/close_debug_mode.jpg?raw=true) +![close_debug_mode.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/close_debug_mode.jpg?raw=true) La ventana de la aplicación se divide en 3 secciones verticales: -![opentrons_app_mainwindow.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_app_mainwindow.jpg?raw=true) +![opentrons_app_mainwindow.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_app_mainwindow.jpg?raw=true) - A la izquierda del todo tenemos un menú que nos permite cambiar entre las ventanas de robot, protocolo, calibración y ejecución. -![opentrons_app_leftmenu.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_app_leftmenu.jpg?raw=true) +![opentrons_app_leftmenu.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_app_leftmenu.jpg?raw=true) - En el panel del medio tenemos las posibles órdenes que podemos dar al robot desde la ventana en la que nos encontramos. -![opentrons_app_middlemenu.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_app_middlemenu.jpg?raw=true) +![opentrons_app_middlemenu.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_app_middlemenu.jpg?raw=true) - En el panel de la derecha, el más grande de los tres, se presenta la información para el usuario así como opciones de configuración e interacción con el robot. Como la anterior, depende de la ventana en la que nos encontremos el contenido de este panel variará. -![opentrons_app_rightmenu.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_app_rightmenu.jpg?raw=true) +![opentrons_app_rightmenu.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_app_rightmenu.jpg?raw=true) ## Conectar un robot @@ -90,15 +90,15 @@ En la aplicación hacemos click en la primera opción del menú lateral de la iz **Nota**: Los robots tipo **C** no tienen luces internas. -![opentrons_app_mainrobot.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_app_mainrobot.jpg?raw=true) +![opentrons_app_mainrobot.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_app_mainrobot.jpg?raw=true) Para poder operar el robot, tendemos que dar la orden de conectarnos a él. Para ello, haz click en la pequeña palanca a la dereha del nombre del robot en el panel de en medio o en el botón `Connect` en el panel de la derecha tras haber seleccionado el robot en el del medio. - ![opentrons_app_connecttorobot.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_app_connecttorobot.jpg?raw=true) + ![opentrons_app_connecttorobot.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_app_connecttorobot.jpg?raw=true) Un baner verde en la parte superior del tercer panel nos confirmará a qué robot nos hemos conectado con éxito. - ![opentrons_app_connectedrobot.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_app_connectedrobot.jpg?raw=true) + ![opentrons_app_connectedrobot.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_app_connectedrobot.jpg?raw=true) **Nota**: Solo puedes estar conectado a un robot al mismo tiempo. Los demás seguirán ejecutando sus órdenes aunque te desconectes de ellos, pero cualquier configuración hecha en un robot corre el riesgo de perderse si te desconectas antes de ejecutar una orden, por lo que es recomendable que en caso de desconexión vuelvas a empezar la configuración de tu protocolo desde el principio. @@ -110,7 +110,7 @@ Para poder operar el robot, tendemos que dar la orden de conectarnos a él. Para Esta es la segunda opción del panel de la izquierda: `Protocol`. Cuando accedas a esta ventana, en el panel de la derecha verás el protocolo que se encuentra actualmente cargado en el robot de la última ejecución. -![opentrons_app_mainprotocol.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_app_mainprotocol.jpg?raw=true) +![opentrons_app_mainprotocol.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_app_mainprotocol.jpg?raw=true) Si quieres cargar un nuevo protocolo, tan solo busca el archivo `.py` correspondiente y arrástralo al panel del medio o haz click en el botón `Open` del mismo panel y selecciona el archivo deseado. @@ -130,7 +130,7 @@ En el panel de la derecha podemos ver el nombre del protocolo, versión, autores En la tercera opción del menú del panel de la izquierda, `Calibrate`, podremos ajustar la posición del brazo del robot y sus movimientos para que se mueva por la cabina correctamente y reconozca el labware al milímetro. -![opentrons_app_maincalibrate.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_app_maincalibrate.jpg?raw=true) +![opentrons_app_maincalibrate.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_app_maincalibrate.jpg?raw=true) **Solo es necesario calibrar un protocolo si:** - es la primera vez que se ejecuta ese protocolo en este robot @@ -149,7 +149,7 @@ Hay que iniciar la calibración con la cabina vacía (a exepción de los módulo Al iniciar la calibración se empezará por las pipetas. Te pedirá paso a paso que quites el cubo de basura de las puntas y pongas una punta en las pipetas, y él solo tocará unos sensores normalemente ocultos bajo el cubo para comprobar el movimiento y posicionamiento de las puntas. Una vez termine el proceso para ambas pipetas, vuelve a poner cuidadosamente el cubo para cubrir los sonsores. -![opentrons_calibration_metalpins.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_calibration_metalpins.jpg?raw=true) +![opentrons_calibration_metalpins.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_calibration_metalpins.jpg?raw=true) **Nota**: Los sensores son unas plaquitas de metal muy delicadas y sensibles. Ten mucho cuidado al quitar y poner el cubo de basura o podrías dañarlos. @@ -161,11 +161,11 @@ Al iniciar la calibración se empezará por las pipetas. Te pedirá paso a paso Una vez acabes de calibrar las pipetas y pases al primer labware, las puntas, te aparecerá una plantilla con la colocación en el deck de los módulos (si los hubiera) y del labware. Es hora de colocarlo todo tal y como se indica en la plantilla, pero todo vacío. Sigue las instrucciones de la sección `Colocar el labware en la cabina` de este manual para hacerlo correctamente. Solo estamos calibrando por el momento y si hubiera líquidos se podría salpicar, manchar o contaminar el labware y la cabina. -![opentrons_calibration_labwarelayout.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_calibration_labwarelayout.jpg?raw=true) +![opentrons_calibration_labwarelayout.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_calibration_labwarelayout.jpg?raw=true) Ahora vamos a comenzar a calibrar las cajas de puntas. El robot desplazará la pipeta correspondiente sobre la punta superior izquierda de cada caja, y usando los controles de la aplicación debe colocarse la pipeta centrada sobre la punta y a ras de su parte superior (a la altura donde acaba la punta debe comenzar la pipeta). Cuando estemos en posición probaremos a coger la punta con la pipeta y si la ha cogido correctamente ese tiprack estará calibrado. Si falla habrá que repetir el proceso hasta que lo haga correctamente. -![opentrons_calibration_controls.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_calibration_controls.jpg?raw=true) +![opentrons_calibration_controls.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_calibration_controls.jpg?raw=true) **Nota**: La pipeta monocanal de 1000µl puede hacer sacudidas extrañas al recoger la punta. Esto es un comportamiento que no depende del robot sino de la aplicación, y puede desactivarse dentro de la aplicacción en la ventana `Robot`, en la sección `PIPETTES & MODULES` del panel central. Selecciona el botón `SETTINGS` correspondiente a la pipeta y habrá un checkbox con la opción de `shaking` que se puede desactivar. @@ -187,11 +187,11 @@ Para colocar el labware dentro de la cabina, sigue las instrucciones que te han - Coloca los botes y cajas tapados siempre que sea posible, y ábrelos solo en el momento antes de la ejecución. - Cuando montes un rack de Opentrons, asegúrate que la esquina marcada del rack coincide con la marca en el soporte. Esa esquina corresponderá con la esquina superior izquierda de la casilla en el deck. -![opentrons_labware_opentronsrack.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_labware_opentronsrack.jpg?raw=true) +![opentrons_labware_opentronsrack.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_labware_opentronsrack.jpg?raw=true) - Para colocar una pieza de labware en su casilla, asegurate de que primero colocas la esquina inferior derecha bien encajada en las pestañas de sujección. El resto debería encajar solo al soltarlo suavemente. -![opentrons_labware_deckslot.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_labware_deckslot.jpg?raw=true) +![opentrons_labware_deckslot.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_labware_deckslot.jpg?raw=true) - Asegurate de que colocas los tubos correspondientes en sus posiciones correctas. - Coloca todas las cajas de puntas como si el protocolo se fuera a ejecutar para el máximo de muestras y pasos. @@ -204,7 +204,7 @@ La última opción del menú de la izquierda, `Run`, nos permite darle la orden Esta orden iniciará la ejecución del protocolo cargado. Asegúrate de que el protocolo cargado es el adecuado, de que estás operando el robot correcto, que el labware está colocado y destapado, los módulos requeridos encendidos y las pipetas sin puntas puestas antes de iniciar la ejecución. -![opentrons_run_start.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_run_start.jpg?raw=true) +![opentrons_run_start.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_run_start.jpg?raw=true) - Pausa (`PAUSE`): @@ -212,13 +212,13 @@ Detiene temporalmente la ejecución del protocolo. Puede tardar unos segundos en **Nota**: En caso de que el robot encuentre un error subsanable, como por ejemplo apertura de la puerta, falta de puntas o basura llena, detendrá la ejecución como si el botón pausa (`PAUSE`) se hubiera pulsado. Esta situación se notifica al operador tanto por texto en el log como con un mensaje sonoro. Una vez el problema ha sido resuelto, pulsando el botón reanudar (`RESUME`) retomará la ejecución. -![opentrons_run_pause.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_run_pause.jpg?raw=true) +![opentrons_run_pause.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_run_pause.jpg?raw=true) - Reanudar (`RESUME`): Retoma la ejecución tras una parada. -![opentrons_run_pauserestart.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_run_pauserestart.jpg?raw=true) +![opentrons_run_pauserestart.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_run_pauserestart.jpg?raw=true) - Cancelar ejecución (`CANCEL RUN`): @@ -226,13 +226,13 @@ Aborta la ejecución del protocolo. Esta orden requiere confirmación y no es re **Nota**: Tras una cancelación, el robot borrará toda información sobre la ejecución fallida, por lo que es recomendable recargar todas las puntas antes de volver a ejecutar. -![opentrons_run_cancelrunconfirmation.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_run_cancelrunconfirmation.jpg?raw=true) +![opentrons_run_cancelrunconfirmation.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_run_cancelrunconfirmation.jpg?raw=true) - Resetear protocolo (`RESET RUN`): Después de terminar una ejecución el robot se detendrá. El color del baner superior y su mensaje nos indicarán si terminó con éxito (verde), quedo pausada (amarillo) o fue abortada por el usuario o un error irrecuperable (rojo). Para ejecutar de nuevo el mismo protocolo no es necesario volver a subir el protocolo, basta con pulsar este botón y preparará el robot para una nueva ejecución del mismo protocolo. -![opentrons_run_resetrun.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/master/img/opentrons_run_resetrun.jpg?raw=true) +![opentrons_run_resetrun.jpg](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/img/opentrons_run_resetrun.jpg?raw=true) En todo momento, en el panel de la derecha de esta ventana veremos un log de los pasos del protocolo. Cuando esté en ejecución, el log irá acanzando y resaltará en azul la orden que se encuentra realizando el robot en ese momento, en gris las ya realizadas y en negro las que quedan para terminar la ejecución. diff --git a/ot2-modifications/MagdeckV1_ElevatorFrame/Readme.md b/ot2-modifications/MagdeckV1_ElevatorFrame/Readme.md index e69de29..0c2932a 100644 --- a/ot2-modifications/MagdeckV1_ElevatorFrame/Readme.md +++ b/ot2-modifications/MagdeckV1_ElevatorFrame/Readme.md @@ -0,0 +1,15 @@ +## OT2 Magdeck V1 Elevator Frame + +The magnetic module V1 is too strong and attracts magnetic beads even when disengaged. But the V2 version is too weak, so we need to fix tinker around to fix this. + +We have created a 3D printable elevator frame designed to raise the plate above the magnetic field of the magnetic module when disengaged. + +The printable file is "OT2 Magedck V1 Elevator Frame.stl", in this same folder, and can be also found in Tinkercad. It is licensed under Atribution 3.0 (CC-BY 3.0). + +![Real picture](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/ot2-modifications/MagdeckV1_ElevatorFrame/OT2_MagdeckV1_ElevatorFrame_picture.jpg?raw=true) + +![3D](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/ot2-modifications/MagdeckV1_ElevatorFrame/OT2_MagdeckV1_ElevatorFrame_3D.jpg?raw=true) + +In case you want the original template, here it is: + +![Template](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/ot2-modifications/MagdeckV1_ElevatorFrame/OT2_MagedckV1_ElevatorFrame_template.jpg?raw=true) From 0e61e41f30a813abe629de8fc3a8c6e2cb2f08b1 Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Sat, 23 May 2020 11:15:52 +0200 Subject: [PATCH 03/95] FIxed file extention --- ...me_3D.JPG => OT2_MagdeckV1_ElevatorFrame_3D.jpg} | Bin 1 file changed, 0 insertions(+), 0 deletions(-) rename ot2-modifications/MagdeckV1_ElevatorFrame/{OT2_MagdeckV1_ElevatorFrame_3D.JPG => OT2_MagdeckV1_ElevatorFrame_3D.jpg} (100%) diff --git a/ot2-modifications/MagdeckV1_ElevatorFrame/OT2_MagdeckV1_ElevatorFrame_3D.JPG b/ot2-modifications/MagdeckV1_ElevatorFrame/OT2_MagdeckV1_ElevatorFrame_3D.jpg similarity index 100% rename from ot2-modifications/MagdeckV1_ElevatorFrame/OT2_MagdeckV1_ElevatorFrame_3D.JPG rename to ot2-modifications/MagdeckV1_ElevatorFrame/OT2_MagdeckV1_ElevatorFrame_3D.jpg From cbb966fc3f0b63b222300cfe3d19ad905febf913 Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Sat, 23 May 2020 11:21:15 +0200 Subject: [PATCH 04/95] Added link to tinkercad --- ot2-modifications/MagdeckV1_ElevatorFrame/Readme.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/ot2-modifications/MagdeckV1_ElevatorFrame/Readme.md b/ot2-modifications/MagdeckV1_ElevatorFrame/Readme.md index 0c2932a..b8f3d8b 100644 --- a/ot2-modifications/MagdeckV1_ElevatorFrame/Readme.md +++ b/ot2-modifications/MagdeckV1_ElevatorFrame/Readme.md @@ -4,7 +4,7 @@ The magnetic module V1 is too strong and attracts magnetic beads even when disen We have created a 3D printable elevator frame designed to raise the plate above the magnetic field of the magnetic module when disengaged. -The printable file is "OT2 Magedck V1 Elevator Frame.stl", in this same folder, and can be also found in Tinkercad. It is licensed under Atribution 3.0 (CC-BY 3.0). +The printable file is [OT2 Magedck V1 Elevator Frame](https://www.tinkercad.com/things/9f8oLel816s-ot2-magedck-v1-elevator-frame), you can find the .stl in this same folder, and can be also found in Tinkercad. It is licensed under Atribution 3.0 (CC-BY 3.0). ![Real picture](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/ot2-modifications/MagdeckV1_ElevatorFrame/OT2_MagdeckV1_ElevatorFrame_picture.jpg?raw=true) From 6b24270daab0a7b65794095a3f2b22a028a65437 Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Sat, 23 May 2020 11:32:28 +0200 Subject: [PATCH 05/95] Typo --- ot2-modifications/MagdeckV1_ElevatorFrame/Readme.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/ot2-modifications/MagdeckV1_ElevatorFrame/Readme.md b/ot2-modifications/MagdeckV1_ElevatorFrame/Readme.md index b8f3d8b..4993e4f 100644 --- a/ot2-modifications/MagdeckV1_ElevatorFrame/Readme.md +++ b/ot2-modifications/MagdeckV1_ElevatorFrame/Readme.md @@ -4,7 +4,7 @@ The magnetic module V1 is too strong and attracts magnetic beads even when disen We have created a 3D printable elevator frame designed to raise the plate above the magnetic field of the magnetic module when disengaged. -The printable file is [OT2 Magedck V1 Elevator Frame](https://www.tinkercad.com/things/9f8oLel816s-ot2-magedck-v1-elevator-frame), you can find the .stl in this same folder, and can be also found in Tinkercad. It is licensed under Atribution 3.0 (CC-BY 3.0). +The printable file is [OT2 Magdeck V1 Elevator Frame](https://www.tinkercad.com/things/9f8oLel816s-ot2-magdeck-v1-elevator-frame), you can find the .stl in this same folder, and can be also found in Tinkercad. It is licensed under Atribution 3.0 (CC-BY 3.0). ![Real picture](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/ot2-modifications/MagdeckV1_ElevatorFrame/OT2_MagdeckV1_ElevatorFrame_picture.jpg?raw=true) From cdd0904ef53c3737d9727fbb36c9c3cceb24db9e Mon Sep 17 00:00:00 2001 From: saramonzon Date: Mon, 25 May 2020 12:53:13 +0200 Subject: [PATCH 06/95] try one new protocol for using m20 in protocol C --- ...tationC_protocol2_pcrmulti_S3.ot2.apiv2.py | 375 ++++++++++++++++++ 1 file changed, 375 insertions(+) create mode 100644 protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py diff --git a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py new file mode 100644 index 0000000..c6a69b4 --- /dev/null +++ b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py @@ -0,0 +1,375 @@ +from opentrons import protocol_api +from opentrons.types import Point +from opentrons.drivers.rpi_drivers import gpio +import time +import math +import os +import subprocess +import json +from datetime import datetime + +# Metadata +metadata = { + 'protocolName': 'S3 Station C Protocol 1 pcr Version 1', + 'author': 'Nick , Sara , Miguel ', + 'source': 'Custom Protocol Request', + 'apiLevel': '2.3' +} + +# Parameters to adapt the protocol +# Warning writing any Parameters below this line. +# It will be deleted if opentronsWeb is used. + +NUM_SAMPLES = 96 +PCR_LABWARE = 'opentrons aluminum nest plate' +ELUTION_LABWARE = 'opentrons aluminum nest plate' +VOLUME_ELUTION = 7 +LANGUAGE = 'esp' +RESET_TIPCOUNT = False + +# End Parameters to adapt the protocol +ACTION = "StationC-protocol1-pcr" +PROTOCOL_ID = "0000-AA" + +## global vars +## initialize robot object +robot = None +# default var for drop tip switching +switch = True +# initialize tip_log dictionary +tip_log = {} +tip_log['count'] = {} +tip_log['tips'] = {} +tip_log['max'] = {} + +""" +NUM_SAMPLES is the number of samples, must be an integer number + +PCR_LABWARE must be one of the following: + opentrons aluminum biorad plate + opentrons aluminum nest plate + opentrons aluminum strip short + covidwarriors aluminum biorad plate + covidwarriors aluminum biorad strip short + +ELUTION_LABWARE must be one of the following: + opentrons plastic 2ml tubes + opentrons plastic 1.5ml tubes + opentrons aluminum 2ml tubes + opentrons aluminum 1.5ml tubes + covidwarriors aluminum 2ml tubes + covidwarriors aluminum 1.5ml tubes + opentrons aluminum biorad plate + opentrons aluminum nest plate + covidwarriors aluminum biorad plate + opentrons aluminum strip alpha + opentrons aluminum strip short + covidwarriors aluminum biorad strip alpha + covidwarriors aluminum biorad strip short + +""" + +# Constants +PCR_LW_DICT = { + 'opentrons aluminum biorad plate': 'opentrons_96_aluminumblock_biorad_wellplate_200ul', + 'opentrons aluminum nest plate': 'opentrons_96_aluminumblock_nest_wellplate_100ul', + 'opentrons aluminum strip short': 'opentrons_aluminumblock_96_pcrstrips_100ul', + 'covidwarriors aluminum biorad plate': 'covidwarriors_aluminumblock_96_bioradwellplate_200ul', + 'covidwarriors aluminum biorad strip short': 'covidwarriors_aluminumblock_96_bioradwellplate_pcrstrips_100ul' +} + +EL_LW_DICT = { + # PCR plate + 'opentrons aluminum biorad plate': 'opentrons_96_aluminumblock_biorad_wellplate_200ul', + 'opentrons aluminum nest plate': 'opentrons_96_aluminumblock_nest_wellplate_100ul', + 'covidwarriors aluminum biorad plate': 'covidwarriors_aluminumblock_96_bioradwellplate_200ul', + # Strips + #'large strips': 'opentrons_96_aluminumblock_generic_pcr_strip_200ul', + #'short strips': 'opentrons_96_aluminumblock_generic_pcr_strip_200ul', + 'opentrons aluminum strip alpha': 'opentrons_aluminumblock_96_pcrstripsalpha_200ul', + 'opentrons aluminum strip short': 'opentrons_aluminumblock_96_pcrstrips_100ul', + 'covidwarriors aluminum biorad strip alpha': 'covidwarriors_aluminumblock_96_bioradwellplate_pcrstripsalpha_200ul', + 'covidwarriors aluminum biorad strip short': 'covidwarriors_aluminumblock_96_bioradwellplate_pcrstrips_100ul' +} + +LANGUAGE_DICT = { + 'esp': 'esp', + 'eng': 'eng' +} + +if LANGUAGE_DICT[LANGUAGE] == 'eng': + VOICE_FILES_DICT = { + 'start': './data/sounds/started_process.mp3', + 'finish': './data/sounds/finished_process.mp3', + 'close_door': './data/sounds/close_door.mp3', + 'replace_tipracks': './data/sounds/replace_tipracks.mp3', + 'empty_trash': './data/sounds/empty_trash.mp3' + } +elif LANGUAGE_DICT[LANGUAGE] == 'esp': + VOICE_FILES_DICT = { + 'start': './data/sounds/started_process_esp.mp3', + 'finish': './data/sounds/finished_process_esp.mp3', + 'close_door': './data/sounds/close_door_esp.mp3', + 'replace_tipracks': './data/sounds/replace_tipracks_esp.mp3', + 'empty_trash': './data/sounds/empty_trash_esp.mp3' + } + + +# Function definitions +# Function definitions +def run_info(start,end,parameters = dict()): + info = {} + hostname = subprocess.run( + ['hostname'], + stdout=subprocess.PIPE, + stderr=subprocess.PIPE + ).stdout.decode('utf-8') + + info["RobotID"] = hostname + info["executedAction"] = ACTION + info["ProtocolID"] = PROTOCOL_ID + info["StartRunTime"] = start + info["FinishRunTime"] = end + info["parameters"] = parameters + # write json to file. This is going to be an api post. + #with open('run.json', 'w') as fp: + #json.dump(info, fp,indent=4) + +def check_door(): + return gpio.read_window_switches() + +def confirm_door_is_closed(): + if not robot.is_simulating(): + #Check if door is opened + if check_door() == False: + #Set light color to red and pause + gpio.set_button_light(1,0,0) + robot.pause() + voice_notification('close_door') + time.sleep(5) + confirm_door_is_closed() + else: + #Set light color to green + gpio.set_button_light(0,1,0) + +def start_run(): + voice_notification('start') + gpio.set_button_light(0,1,0) + now = datetime.now() + # dd/mm/YY H:M:S + start_time = now.strftime("%Y/%m/%d %H:%M:%S") + return start_time + +def finish_run(): + voice_notification('finish') + #Set light color to blue + gpio.set_button_light(0,0,1) + now = datetime.now() + # dd/mm/YY H:M:S + finish_time = now.strftime("%Y/%m/%d %H:%M:%S") + return finish_time + +def voice_notification(action): + if not robot.is_simulating(): + fname = VOICE_FILES_DICT[action] + if os.path.isfile(fname) is True: + subprocess.run( + ['mpg123', fname], + stdout=subprocess.PIPE, + stderr=subprocess.PIPE + ) + else: + robot.comment(f"Sound file does not exist. Call the technician") + +def reset_tipcount(file_path = '/data/C/tip_log.json'): + if os.path.isfile(file_path): + os.remove(file_path) + +def retrieve_tip_info(pip,tipracks,file_path = '/data/C/tip_log.json'): + global tip_log + if not tip_log['count'] or pip not in tip_log['count']: + tip_log['count'][pip] = 0 + if not robot.is_simulating(): + if os.path.isfile(file_path): + with open(file_path) as json_file: + data = json.load(json_file) + if 'P1000' in str(pip): + tip_log['count'][pip] = data['tips1000'] + elif 'P300' in str(pip): + tip_log['count'][pip] = data['tips300'] + elif 'P20' in str(pip): + tip_log['count'][pip] = data['tips20'] + + if "8-Channel" in str(pip): + tip_log['tips'][pip] = [tip for rack in tipracks for tip in rack.rows()[0]] + else: + tip_log['tips'][pip] = [tip for rack in tipracks for tip in rack.wells()] + + tip_log['max'][pip] = len(tip_log['tips'][pip]) + + return tip_log + +def save_tip_info(file_path = '/data/C/tip_log.json'): + data = {} + if not robot.is_simulating(): + if os.path.isfile(file_path): + os.rename(file_path,file_path + ".bak") + for pip in tip_log['count']: + if "P1000" in str(pip): + data['tips1000'] = tip_log['count'][pip] + elif "P300" in str(pip): + data['tips300'] = tip_log['count'][pip] + elif "P20" in str(pip): + data['tips20'] = tip_log['count'][pip] + + with open(file_path, 'a+') as outfile: + json.dump(data, outfile) + +def pick_up(pip,tiprack): + if tip_log['count'][pip] == tip_log['max'][pip]: + voice_notification('replace_tipracks') + robot.pause('Replace ' + str(pip.max_volume) + 'µl tipracks before \ +resuming.') + confirm_door_is_closed() + pip.reset_tipracks() + tip_log['count'][pip] = 0 + pip.pick_up_tip(tip_log['tips'][pip][tip_log['count'][pip]]) + tip_log['count'][pip] += 1 + +def drop(pip): + global switch + if "8-Channel" not in str(pip): + side = 1 if switch else -1 + drop_loc = robot.loaded_labwares[12].wells()[0].top().move(Point(x=side*20)) + pip.drop_tip(drop_loc,home_after=False) + switch = not switch + else: + drop_loc = robot.loaded_labwares[12].wells()[0].top().move(Point(x=20)) + pip.drop_tip(drop_loc,home_after=False) + +def get_source_dest_coordinates(source_racks, pcr_plate): + num_cols = math.ceil(NUM_SAMPLES/8) + if 'strip' in ELUTION_LABWARE: + sources = [ + col + for i, rack in enumerate(source_racks) + for col in [ + rack.columns()[c] if i < 2 else rack.columns()[c+1] + for c in [0, 5, 10] + ] + ][:NUM_SAMPLES] + + elif 'plate' in ELUTION_LABWARE: + sources = source_racks.rows()[:num_cols] + dests = pcr_plate.rows()[:num_cols] + return sources, dests + +def transfer_samples(sources, dests, pip,tiprack): + # height for aspiration has to be different depending if you ar using tubes or wells + if 'strip' in ELUTION_LABWARE or 'plate' in ELUTION_LABWARE: + height = 1.5 + else: + height = 1 + # transfer + for s, d in zip(sources, dests): + # Skip for negative control, position NUM_SAMPLES-2 + if s == sources[NUM_SAMPLES-2]: + continue + + pick_up(pip,tiprack) + pip.transfer(VOLUME_ELUTION, s.bottom(height), d.bottom(2), air_gap=2, new_tip='never') + #p20.mix(1, 10, d.bottom(2)) + #p20.blow_out(d.top(-2)) + pip.aspirate(1, d.top(-2)) + drop(pip) + +# RUN PROTOCOL +def run(ctx: protocol_api.ProtocolContext): + global robot + global tip_log + + # Set robot as global var + robot = ctx + + # check if tipcount is being reset + if RESET_TIPCOUNT: + reset_tipcount() + + # confirm door is closed + robot.comment(f"Please, close the door") + confirm_door_is_closed() + + # Begin run + start_time = start_run() + + # define tips + tips20 = [ + robot.load_labware('opentrons_96_filtertiprack_20ul', 6) + ] + tipsm20 = [ + robot.load_labware('opentrons_96_filtertiprack_20ul', 3) + ] + + # define pipettes + p20 = robot.load_instrument('p20_single_gen2', 'right', tip_racks=tips20) + m20 = robot.load_instrument('p20_multi_gen2', 'left', tip_racks=tips20) + + ## retrieve tip_log + retrieve_tip_info(p20,tips20) + retrieve_tip_info(p300,tips300) + + # tempdeck module + tempdeck = robot.load_module('tempdeck', '10') + tempdeck.set_temperature(4) + + # check pcr plate + if PCR_LABWARE not in PCR_LW_DICT: + raise Exception('Invalid PCR_LABWARE. Must be one of the following:\n' + '\n'.join(list(PCR_LW_DICT.keys()))) + + + # load pcr plate + pcr_plate = tempdeck.load_labware( + PCR_LW_DICT[PCR_LABWARE], 'PCR plate') + + # check source (elution) labware type + if ELUTION_LABWARE not in EL_LW_DICT: + raise Exception('Invalid ELUTION_LABWARE. Must be one of the following:\n' + '\n'.join(list(EL_LW_DICT.keys()))) + + # load elution labware + if 'plate' in ELUTION_LABWARE: + source_racks = robot.load_labware( + EL_LW_DICT[ELUTION_LABWARE], '1', + 'RNA elution labware') + else: + source_racks = [ + robot.load_labware(EL_LW_DICT[ELUTION_LABWARE], slot, + 'RNA elution labware ' + str(i+1)) + for i, slot in enumerate(['4', '1', '5', '2']) + ] + + # setup sample sources and destinations + sources, dests = get_source_dest_coordinates(source_racks, pcr_plate) + + # transfer samples to corresponding locations + transfer_samples_m(sources, dests, m20,tipsm20) + + # transfer negative control to position NUM_SAMPLES-2 + pick_up(p20, tips20) + p20.transfer(VOLUME_ELUTION, mm_rack.wells()[4].bottom(1), dests[NUM_SAMPLES-2].bottom(2), air_gap=2, new_tip='never') + drop(p20) + + # track final used tip + save_tip_info() + + finish_time = finish_run() + + par = { + "NUM_SAMPLES" : NUM_SAMPLES, + "PCR_LABWARE" : PCR_LABWARE, + "ELUTION_LABWARE" : ELUTION_LABWARE, + "VOLUME_ELUTION" : VOLUME_ELUTION, + "LANGUAGE" : LANGUAGE, + "RESET_TIPCOUNT" : RESET_TIPCOUNT + } + + run_info(start_time, finish_time, par) From edf878fc7c288dcc2022f8831ec48c3ed5a4dfe6 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Mon, 25 May 2020 13:14:43 +0200 Subject: [PATCH 07/95] modification for using multi in complete columns and remaining samples with p20 --- ...tationC_protocol2_pcrmulti_S3.ot2.apiv2.py | 36 ++++++++++++++----- 1 file changed, 28 insertions(+), 8 deletions(-) diff --git a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py index c6a69b4..5fa823d 100644 --- a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py @@ -248,21 +248,39 @@ def drop(pip): pip.drop_tip(drop_loc,home_after=False) def get_source_dest_coordinates(source_racks, pcr_plate): - num_cols = math.ceil(NUM_SAMPLES/8) + # Keep floor + num_cols = math.floor(NUM_SAMPLES/8) + # Keep the remaining samples + num_samples_m = (num_cols*8) + remainder = math.remainder(NUM_SAMPLES,8) + if 'strip' in ELUTION_LABWARE: - sources = [ - col + if NUM_SAMPLES > 8: + sources_m = [ + col + for i, rack in enumerate(source_racks) + for col in [ + rack.columns()[c] if i < 2 else rack.columns()[c+1] + for c in [0, 5, 10] + ] + ][:num_cols] + + sources_s = [ + well for i, rack in enumerate(source_racks) for col in [ rack.columns()[c] if i < 2 else rack.columns()[c+1] for c in [0, 5, 10] ] - ][:NUM_SAMPLES] + for well in col + ][num_samples_m:remainder] elif 'plate' in ELUTION_LABWARE: sources = source_racks.rows()[:num_cols] - dests = pcr_plate.rows()[:num_cols] - return sources, dests + + dests_m = pcr_plate.rows()[:num_cols] + dests_s = pcr_plate.wells()[num_samples_m:remainder] + return sources_m, sources_s, dests_m, dests_s def transfer_samples(sources, dests, pip,tiprack): # height for aspiration has to be different depending if you ar using tubes or wells @@ -348,10 +366,12 @@ def run(ctx: protocol_api.ProtocolContext): ] # setup sample sources and destinations - sources, dests = get_source_dest_coordinates(source_racks, pcr_plate) + sources_m, sources_s, dests_m, dests_s = get_source_dest_coordinates(source_racks, pcr_plate) # transfer samples to corresponding locations - transfer_samples_m(sources, dests, m20,tipsm20) + if NUM_SAMPLES > 8: + transfer_samples(sources_m,dests_m,m20,tipsm20) + transfer_samples(sources_s, dests_s, p20,tips20) # transfer negative control to position NUM_SAMPLES-2 pick_up(p20, tips20) From 69e723809bde332e1c8007907e0c4140f0c02341 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Mon, 25 May 2020 13:17:03 +0200 Subject: [PATCH 08/95] fixed retrieve_tip_info for m20 --- protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py index 5fa823d..98dab25 100644 --- a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py @@ -334,7 +334,7 @@ def run(ctx: protocol_api.ProtocolContext): ## retrieve tip_log retrieve_tip_info(p20,tips20) - retrieve_tip_info(p300,tips300) + retrieve_tip_info(m20,tipsm20) # tempdeck module tempdeck = robot.load_module('tempdeck', '10') From f78aae9af07bcc5ea5d004c43749b5eb1cf84f60 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Mon, 25 May 2020 13:35:44 +0200 Subject: [PATCH 09/95] fixed errors seeing in simulation --- ...tationC_protocol2_pcrmulti_S3.ot2.apiv2.py | 37 ++++++++++++++++--- 1 file changed, 31 insertions(+), 6 deletions(-) diff --git a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py index 98dab25..12e2a73 100644 --- a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py @@ -22,6 +22,7 @@ NUM_SAMPLES = 96 PCR_LABWARE = 'opentrons aluminum nest plate' +MM_LABWARE = 'opentrons aluminum block' ELUTION_LABWARE = 'opentrons aluminum nest plate' VOLUME_ELUTION = 7 LANGUAGE = 'esp' @@ -45,6 +46,11 @@ """ NUM_SAMPLES is the number of samples, must be an integer number +MM_LABWARE must be one of the following: + opentrons plastic block + pentrons aluminum block + covidwarriors aluminum block + PCR_LABWARE must be one of the following: opentrons aluminum biorad plate opentrons aluminum nest plate @@ -70,6 +76,12 @@ """ # Constants +MM_LW_DICT = { + 'opentrons plastic block': 'opentrons_24_tuberack_generic_2ml_screwcap', + 'opentrons aluminum block': 'opentrons_24_aluminumblock_generic_2ml_screwcap', + 'covidwarriors aluminum block': 'covidwarriors_aluminumblock_24_screwcap_2000ul' +} + PCR_LW_DICT = { 'opentrons aluminum biorad plate': 'opentrons_96_aluminumblock_biorad_wellplate_200ul', 'opentrons aluminum nest plate': 'opentrons_96_aluminumblock_nest_wellplate_100ul', @@ -251,8 +263,8 @@ def get_source_dest_coordinates(source_racks, pcr_plate): # Keep floor num_cols = math.floor(NUM_SAMPLES/8) # Keep the remaining samples - num_samples_m = (num_cols*8) - remainder = math.remainder(NUM_SAMPLES,8) + num_samples_m = int(num_cols*8) + remainder = int(math.remainder(NUM_SAMPLES,8)) if 'strip' in ELUTION_LABWARE: if NUM_SAMPLES > 8: @@ -276,9 +288,10 @@ def get_source_dest_coordinates(source_racks, pcr_plate): ][num_samples_m:remainder] elif 'plate' in ELUTION_LABWARE: - sources = source_racks.rows()[:num_cols] + sources_m = source_racks.rows()[0][:num_cols] + sources_s = source_racks.wells()[num_samples_m:remainder] - dests_m = pcr_plate.rows()[:num_cols] + dests_m = pcr_plate.rows()[0][:num_cols] dests_s = pcr_plate.wells()[num_samples_m:remainder] return sources_m, sources_s, dests_m, dests_s @@ -291,8 +304,8 @@ def transfer_samples(sources, dests, pip,tiprack): # transfer for s, d in zip(sources, dests): # Skip for negative control, position NUM_SAMPLES-2 - if s == sources[NUM_SAMPLES-2]: - continue + #if s == sources[NUM_SAMPLES-2]: + # continue pick_up(pip,tiprack) pip.transfer(VOLUME_ELUTION, s.bottom(height), d.bottom(2), air_gap=2, new_tip='never') @@ -328,6 +341,16 @@ def run(ctx: protocol_api.ProtocolContext): robot.load_labware('opentrons_96_filtertiprack_20ul', 3) ] + if MM_LABWARE not in MM_LW_DICT: + raise Exception('Invalid MM_LABWARE. Must be one of the following:\n' + '\n'.join(list(MM_LW_DICT.keys()))) + + + # load mastermix labware + mm_rack = robot.load_labware( + MM_LW_DICT[MM_LABWARE], '11', + MM_LABWARE) + + # define pipettes p20 = robot.load_instrument('p20_single_gen2', 'right', tip_racks=tips20) m20 = robot.load_instrument('p20_multi_gen2', 'left', tip_racks=tips20) @@ -375,6 +398,7 @@ def run(ctx: protocol_api.ProtocolContext): # transfer negative control to position NUM_SAMPLES-2 pick_up(p20, tips20) + dests = pcr_plate.wells()[:NUM_SAMPLES] p20.transfer(VOLUME_ELUTION, mm_rack.wells()[4].bottom(1), dests[NUM_SAMPLES-2].bottom(2), air_gap=2, new_tip='never') drop(p20) @@ -385,6 +409,7 @@ def run(ctx: protocol_api.ProtocolContext): par = { "NUM_SAMPLES" : NUM_SAMPLES, + "MM_LABWARE" : MM_LABWARE, "PCR_LABWARE" : PCR_LABWARE, "ELUTION_LABWARE" : ELUTION_LABWARE, "VOLUME_ELUTION" : VOLUME_ELUTION, From e7b5e70e3107c6de594c5ae9242d2432bab062f3 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Mon, 25 May 2020 16:12:44 +0200 Subject: [PATCH 10/95] fixed error, changed remainder for NUM_SAMPLES --- protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py | 7 +++---- 1 file changed, 3 insertions(+), 4 deletions(-) diff --git a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py index 12e2a73..cbaa094 100644 --- a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py @@ -264,7 +264,6 @@ def get_source_dest_coordinates(source_racks, pcr_plate): num_cols = math.floor(NUM_SAMPLES/8) # Keep the remaining samples num_samples_m = int(num_cols*8) - remainder = int(math.remainder(NUM_SAMPLES,8)) if 'strip' in ELUTION_LABWARE: if NUM_SAMPLES > 8: @@ -285,14 +284,14 @@ def get_source_dest_coordinates(source_racks, pcr_plate): for c in [0, 5, 10] ] for well in col - ][num_samples_m:remainder] + ][num_samples_m:NUM_SAMPLES] elif 'plate' in ELUTION_LABWARE: sources_m = source_racks.rows()[0][:num_cols] - sources_s = source_racks.wells()[num_samples_m:remainder] + sources_s = source_racks.wells()[num_samples_m:NUM_SAMPLES] dests_m = pcr_plate.rows()[0][:num_cols] - dests_s = pcr_plate.wells()[num_samples_m:remainder] + dests_s = pcr_plate.wells()[num_samples_m:NUM_SAMPLES] return sources_m, sources_s, dests_m, dests_s def transfer_samples(sources, dests, pip,tiprack): From 5bf9a1499247d91a32669eb23dbed1c3a33411f7 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Mon, 25 May 2020 16:56:31 +0200 Subject: [PATCH 11/95] create config scripts for S3-C3 --- configuration_scripts/S3/rename_robots/S3-C3 | 18 ++++++++++ .../S3/static_ips/S3-C3_static_ip.py | 33 +++++++++++++++++++ 2 files changed, 51 insertions(+) create mode 100644 configuration_scripts/S3/rename_robots/S3-C3 create mode 100644 configuration_scripts/S3/static_ips/S3-C3_static_ip.py diff --git a/configuration_scripts/S3/rename_robots/S3-C3 b/configuration_scripts/S3/rename_robots/S3-C3 new file mode 100644 index 0000000..68811f9 --- /dev/null +++ b/configuration_scripts/S3/rename_robots/S3-C3 @@ -0,0 +1,18 @@ +NEW_SERIAL_NUMBER = "S3-C3" + +from opentrons import robot +import os + +robot.comment(f"Setting serial number to {NEW_SERIAL_NUMBER}.") + +if not robot.is_simulating(): + with open("/var/serial", "w") as serial_number_file: + serial_number_file.write(NEW_SERIAL_NUMBER + "\n") + with open("/etc/machine-info", "w") as serial_number_file: + serial_number_file.write(f"DEPLOYMENT=production\nPRETTY_HOSTNAME={NEW_SERIAL_NUMBER}\n") + with open("/etc/hostname", "w") as serial_number_file: + serial_number_file.write(NEW_SERIAL_NUMBER + "\n") + + os.sync() + + robot.comment("Done.") diff --git a/configuration_scripts/S3/static_ips/S3-C3_static_ip.py b/configuration_scripts/S3/static_ips/S3-C3_static_ip.py new file mode 100644 index 0000000..b4aa342 --- /dev/null +++ b/configuration_scripts/S3/static_ips/S3-C3_static_ip.py @@ -0,0 +1,33 @@ +STATIC_IP = "169.254.100.100/16" + +keyfile_contents = f"""\ +# This file was placed here by Opentrons Support to work around suspected issues with mDNS. +# Normally, IP addresses are assigned dynamically by the "wired-linklocal" or "wired" connection. +# This overrides both of those to set a known, static IP address. +[connection] +id=support-team-wired-static-ip +type=ethernet +autoconnect-priority=20 +interface-name=eth0 +permissions= +[ethernet] +cloned-mac-address=permanent +mac-address-blacklist= +[ipv4] +dns-search= +method=manual +addresses={STATIC_IP} +""" + +from opentrons import robot +import os + +robot.comment(f"Run this protocol to permanently set the wired IP address of your OT-2 to {STATIC_IP}.") + +if not robot.is_simulating(): + with open("/var/lib/NetworkManager/system-connections/support-team-wired-static-ip", "w") as keyfile: + keyfile.write(keyfile_contents) + os.sync() + robot.comment("Done.") + +robot.comment("Restart your OT-2 to apply the changes.") From 13447135adbdf8b930328c3c1bbc14623c7adfc9 Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Tue, 26 May 2020 13:09:38 +0200 Subject: [PATCH 12/95] Always drop tip after mixing mm --- protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py index bc3dbf9..3633aba 100644 --- a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py @@ -349,8 +349,7 @@ def homogenize_mm(mm_tube, pip, tiprack, times=5): pip.dispense(200, mm_tube.bottom(volume_height)) # blow out before dropping tip pip.blow_out(mm_tube.top(-2)) - if VOLUME_MMIX < 20: - drop(pip) + drop(pip) def prepare_mastermix(mm_rack, p300, p20,tiprack300,tiprack20): # setup mastermix coordinates From a826a4944cc251de52d83ef981946d24ce23d709 Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Tue, 26 May 2020 13:20:13 +0200 Subject: [PATCH 13/95] Replacing tip each 3 usages when transferring mm with p20 --- protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py | 8 +++++++- 1 file changed, 7 insertions(+), 1 deletion(-) diff --git a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py index 3633aba..e231682 100644 --- a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py @@ -416,6 +416,7 @@ def transfer_mastermix(mm_tube, dests, p300, p20, tiprack300, tiprack20): # get initial fluid height to avoid overflowing mm when aspiring mm_volume = VOLUME_MMIX * NUM_SAMPLES volume_height = get_mm_height(mm_volume) + dest_count = 0 for set in dest_sets: # check height and if it is low enought, aim for the bottom if volume_height < 5: @@ -429,7 +430,12 @@ def transfer_mastermix(mm_tube, dests, p300, p20, tiprack300, tiprack20): pip.distribute(VOLUME_MMIX, disp_loc, [d.bottom(2) for d in set], air_gap=1, disposal_volume=0, new_tip='never') pip.blow_out(disp_loc) - drop(pip) + dest_count += 1 + if (dest_count % 3 == 0) and pip == p20: + drop(pip) + pick_up(pip,tiprack) + if pip.hw_pipette['has_tip']: + drop(pip) def transfer_samples(sources, dests, pip,tiprack): # height for aspiration has to be different depending if you ar useing tubes or wells From b2baf1ff549efabbba819b09fd690a7f59254c6c Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Tue, 26 May 2020 13:22:33 +0200 Subject: [PATCH 14/95] Only one rack of each tip type is now defined --- protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py | 5 +---- 1 file changed, 1 insertion(+), 4 deletions(-) diff --git a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py index e231682..75c226b 100644 --- a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py @@ -476,10 +476,7 @@ def run(ctx: protocol_api.ProtocolContext): start_time = start_run() # define tips - tips20 = [ - robot.load_labware('opentrons_96_filtertiprack_20ul', slot) - for slot in ['6', '9', '8', '7'] - ] + tips20 = [robot.load_labware('opentrons_96_filtertiprack_20ul', '6')] tips300 = [robot.load_labware('opentrons_96_filtertiprack_200ul', '3')] # define pipettes From c2493f29598bc85da51a626bbafb9be662e39811 Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Tue, 26 May 2020 13:29:01 +0200 Subject: [PATCH 15/95] Added labware ecogen deepwell to protcols A --- protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py | 2 ++ protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py | 2 ++ 2 files changed, 4 insertions(+) diff --git a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py index 94ed5c0..38726c1 100644 --- a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py @@ -53,6 +53,7 @@ opentrons deep generic well plate nest deep generic well plate vwr deep generic well plate + ecogen deep generic well plate """ BD_LW_DICT = { @@ -63,6 +64,7 @@ PL_LW_DICT = { 'opentrons deep generic well plate': 'usascientific_96_wellplate_2.4ml_deep', 'nest deep generic well plate': 'nest_96_deepwellplate_2000ul', + 'ecogen deep generic well plate': 'ecogen_96_deepwellplate_2000ul', 'vwr deep generic well plate': 'vwr_96_deepwellplate_2000ul' } diff --git a/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py index e09c48b..2d4b69d 100644 --- a/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py @@ -58,6 +58,7 @@ opentrons deep generic well plate nest deep generic well plate vwr deep generic well plate + ecogen deep generic well plate """ LY_LW_DICT = { @@ -67,6 +68,7 @@ PL_LW_DICT = { 'opentrons deep generic well plate': 'usascientific_96_wellplate_2.4ml_deep', 'nest deep generic well plate': 'nest_96_deepwellplate_2000ul', + 'ecogen deep generic well plate': 'ecogen_96_deepwellplate_2000ul', 'vwr deep generic well plate': 'vwr_96_deepwellplate_2000ul' } From d153fd1d3bab0ee8023a56eb0ff052f65d44e47f Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Tue, 26 May 2020 13:31:12 +0200 Subject: [PATCH 16/95] PROTOCOL_ID moved into section of variables modified from the web --- protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py | 3 ++- protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py | 3 ++- protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py | 2 +- protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py | 2 +- protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py | 2 +- protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py | 2 +- 6 files changed, 8 insertions(+), 6 deletions(-) diff --git a/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py index 36936c4..872e597 100644 --- a/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py @@ -28,10 +28,11 @@ VOLUME_BUFFER = 300 LANGUAGE = 'esp' RESET_TIPCOUNT = False +PROTOCOL_ID = "0000-AA" # End Parameters to adapt the protocol ACTION = "StationA-protocol1-buffer" -PROTOCOL_ID = "0000-AA" + ## global vars ## initialize robot object diff --git a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py index 38726c1..e747110 100644 --- a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py @@ -27,10 +27,11 @@ DILUTE_BEADS = True LANGUAGE = 'esp' RESET_TIPCOUNT = False +PROTOCOL_ID = "0000-AA" # End Parameters to adapt the protocol ACTION = "StationA-protocol2-beads" -PROTOCOL_ID = "0000-AA" + ## global vars ## initialize robot object robot = None diff --git a/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py index 2d4b69d..873021a 100644 --- a/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py @@ -29,10 +29,10 @@ BEADS = False LANGUAGE = 'esp' RESET_TIPCOUNT = False +PROTOCOL_ID = "0000-AA" # End Parameters to adapt the protocol ACTION = "StationA-protocol3-lysates" -PROTOCOL_ID = "0000-AA" ## global vars ## initialize robot object diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index 66bd6f6..076d37d 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -42,6 +42,7 @@ DISPENSE_BEADS = False LANGUAGE = 'esp' RESET_TIPCOUNT = False +PROTOCOL_ID = "0000-AA" # End Parameters to adapt the protocol @@ -88,7 +89,6 @@ # Config variables ACTION = "StationB-protocol1-extraction" -PROTOCOL_ID = "0000-AA" # Constants REAGENT_LW_DICT = { diff --git a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py index 75c226b..eb4274b 100644 --- a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py @@ -32,10 +32,10 @@ TRANSFER_SAMPLES = True LANGUAGE = 'esp' RESET_TIPCOUNT = False +PROTOCOL_ID = "0000-AA" # End Parameters to adapt the protocol ACTION = "StationC-protocol1-pcr" -PROTOCOL_ID = "0000-AA" ## global vars ## initialize robot object diff --git a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py index cbaa094..1d86bba 100644 --- a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py @@ -27,10 +27,10 @@ VOLUME_ELUTION = 7 LANGUAGE = 'esp' RESET_TIPCOUNT = False +PROTOCOL_ID = "0000-AA" # End Parameters to adapt the protocol ACTION = "StationC-protocol1-pcr" -PROTOCOL_ID = "0000-AA" ## global vars ## initialize robot object From a49c556865a5cbcfcc486a163f26dd5091edc0a9 Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Tue, 26 May 2020 13:40:13 +0200 Subject: [PATCH 17/95] Increased number of mixes and power from x8 to x10 before distributing --- protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py index e747110..5479fc0 100644 --- a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py @@ -247,10 +247,10 @@ def transfer_beads(beads_tube, dests, pip,tiprack): for i in range(len(split_ind)-1)] + [dests[split_ind[-1]:]] # pick_up(pip,tiprack) # Mix bead tubes prior to dispensing - pip.flow_rate.aspirate = 800 - pip.flow_rate.dispense = 8000 + pip.flow_rate.aspirate = 1000 + pip.flow_rate.dispense = 10000 # pip.mix(12,800,beads_tube.bottom(15)) - for i in range(12): + for i in range(15): pip.aspirate(800, beads_tube.bottom(30)) pip.dispense(800, beads_tube.bottom(2)) pip.flow_rate.aspirate = 100 From f1a3289df13d475a62c771d97b49772416498588 Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Tue, 26 May 2020 13:51:23 +0200 Subject: [PATCH 18/95] Changing tips for NUM_SAMPLES > 24 and twice if NUM_SAMPLES > 72 --- .../S3/stationB_protocol1_extraction_S3.ot2.apiv2.py | 8 +++++++- 1 file changed, 7 insertions(+), 1 deletion(-) diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index 076d37d..36b22d2 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -480,7 +480,7 @@ def run(ctx: protocol_api.ProtocolContext): remove_supernatant(mag_samples_s,waste,p1000,tips1000) # empty trash - if NUM_SAMPLES > 48: + if NUM_SAMPLES > 24: voice_notification('empty_trash') robot.pause(f"Please, empty trash") confirm_door_is_closed() @@ -488,6 +488,12 @@ def run(ctx: protocol_api.ProtocolContext): # 3x washes wash(wash_sets,mag_samples_m,waste,magdeck,m300,tips300) + # empty trash + if NUM_SAMPLES > 72: + voice_notification('empty_trash') + robot.pause(f"Please, empty trash") + confirm_door_is_closed() + # elute samples magdeck.disengage() elute_samples(mag_samples_m,elution_samples_m,elution_buffer,magdeck,m300,tips300) From 6448152fa25b02f3a1012312bcd36a7676b63111 Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Tue, 26 May 2020 15:39:49 +0200 Subject: [PATCH 19/95] Added real pictures --- .../OT2_MagdeckV1_ElevatorFrame_onmagdeck.jpg | Bin 0 -> 384478 bytes .../OT2_MagdeckV1_ElevatorFrame_picture.jpg | Bin 0 -> 215297 bytes ...gdeckV1_ElevatorFrame_withdeepwellplate.jpg | Bin 0 -> 324644 bytes .../MagdeckV1_ElevatorFrame/Readme.md | 4 ++++ 4 files changed, 4 insertions(+) create mode 100644 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plate](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/ot2-modifications/MagdeckV1_ElevatorFrame/OT2_MagdeckV1_ElevatorFrame_withdeepwellplate.jpg?raw=true) + +![On magdeck](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/ot2-modifications/MagdeckV1_ElevatorFrame/OT2_MagdeckV1_ElevatorFrame_onmagdeck.jpg?raw=true) + ![3D](https://github.com/BU-ISCIII/opentrons_covid19/blob/develop/ot2-modifications/MagdeckV1_ElevatorFrame/OT2_MagdeckV1_ElevatorFrame_3D.jpg?raw=true) In case you want the original template, here it is: From 074eb2ae0a19c311cd0bdcc39c42de78e310e73c Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Tue, 26 May 2020 15:43:48 +0200 Subject: [PATCH 20/95] Updated STL file --- .../OT2 Magdeck V1 Elevator Frame.stl | Bin 0 -> 87884 bytes .../OT2 Magedck V1 Elevator Frame.stl | Bin 87684 -> 0 bytes 2 files changed, 0 insertions(+), 0 deletions(-) create mode 100644 ot2-modifications/MagdeckV1_ElevatorFrame/OT2 Magdeck V1 Elevator Frame.stl delete mode 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zH07@qX{)B~Alt{RR@$nuC4(Jo6SD~R)xf1K1JPLo#(=L}^egWl9S?E@kVRmfiwLfB zK_1t?vIt!zYaKc~vtpJ-fQz|?QxLp;kLWByPlHhtXhn1u!L*dCV>>imo;U-7Ux3TX zfJeFJrGBH=vj{{V5umwlq~DbZZ7rlL0=wXe;Hpzr2G^Z{=FVKu`3RLkE=6Z$=stbb ziYa9gxCf59D>@&+J^#=|Wf0w123r7?_tm^hF32M~D?|5FANk-GZoD?Zm#+L0#b`jf z6d!6f9Ue-W{_Nm#AhKv&* z+TeQRCp18y?qfUn^aU&19vm{=%OE@;wq=*YLi0&9cO@PRk@y3WXZLfcB z(AtNQ z0U3;#EMoUJ64x`b5~WeuI<&Kk#A}LK#G8k$ZA0lqx{5gQp)cF6*y{EM2)2+wymHZp zZB4!I^ynSCuWGw(+Z!7oR4b;d^0HcKx+;&_W)Z48xgdk+EMh_R-L@}&eYMw}@k#`G z23drb576vC5S>M+3`OMoiuytma&@c~YZ-89jY^MXwPO4L!RQ0csLUcXb{R_@BPdwF7~Sp?r8UAAPPnNk+PI$XIFoki%_K*t|EEuBSh41jbM zosZCTwcnnPP&>#4c|>Qm+U>r*-56({k9NIRjrXl}jL;LHn{=@hm$KyhTpYS%jvmcN{RKEMoPNJzSe-cbV|EFUkNne(^X; z5s(KCAXe|ZyQ_zfXfRXM=u|Wil$VvkbHb`g#|VD^IE~=UJy{>H2e1 Q8`z=a8qhjI0-YrP5BwSXLjV8( From 4ca3da0e519c5aa5834923b593e39033a89d96e5 Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Tue, 26 May 2020 15:48:46 +0200 Subject: [PATCH 21/95] Added reuse tips option prototipe --- ...tionB_protocol1_extraction_S3.ot2.apiv2.py | 36 ++++++++++++++++++- 1 file changed, 35 insertions(+), 1 deletion(-) diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index 36b22d2..9102e4d 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -40,6 +40,7 @@ WASTE_LABWARE = 'nest 1 reservoir plate' ELUTION_LABWARE = 'opentrons aluminum nest plate' DISPENSE_BEADS = False +REUSE_TIPS = True LANGUAGE = 'esp' RESET_TIPCOUNT = False PROTOCOL_ID = "0000-AA" @@ -305,6 +306,36 @@ def remove_supernatant(sources,waste,pip,tiprack): pip.blow_out(waste) drop(pip) +def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack): + for wash_set in wash_sets: + for i, m in enumerate(dests): + # transfer and mix wash with beads + magdeck.disengage() + wash_chan = wash_set[i//6] + side = 1 if i % 2 == 0 else -1 + asp_loc = m.bottom(1.3) + disp_loc = m.bottom(5) + pick_up(pip,tiprack) + pip.transfer( + 200, wash_chan.bottom(2), m.center(), new_tip='never', air_gap=20) + # Mix heigh has to be really close to bottom, it was 5 now reduced to 2, maybe should be 1? + dispense_default_speed = pip.flow_rate.dispense + pip.flow_rate.dispense = 1500 + pip.mix(7, 200, m.bottom(2)) + pip.flow_rate.dispense = dispense_default_speed + + magdeck.engage(height_from_base=MAGNET_HEIGHT) + robot.delay(seconds=75, msg='Incubating on magnet for 75 seconds.') + + # remove supernatant + aspire_default_speed = pip.flow_rate.aspirate + pip.flow_rate.aspirate = 75 + asp_loc = m.bottom(1.5) + pip.transfer(200, asp_loc, waste, new_tip='never', air_gap=20) + pip.flow_rate.aspirate = aspire_default_speed + pip.blow_out(waste) + drop(pip) + def wash(wash_sets,dests,waste,magdeck,pip,tiprack): for wash_set in wash_sets: for i, m in enumerate(dests): @@ -486,7 +517,10 @@ def run(ctx: protocol_api.ProtocolContext): confirm_door_is_closed() # 3x washes - wash(wash_sets,mag_samples_m,waste,magdeck,m300,tips300) + if REUSE_TIPS == True: + wash_reuse(wash_sets,mag_samples_m,waste,magdeck,m300,tips300) + else: + wash(wash_sets,mag_samples_m,waste,magdeck,m300,tips300) # empty trash if NUM_SAMPLES > 72: From aadcebec27adb2c5d7366f7bb7f3b6a7bd13e169 Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Tue, 26 May 2020 16:06:47 +0200 Subject: [PATCH 22/95] Using an empty tiprack for resuing tips and changed p1000 assigned slots --- ...tionB_protocol1_extraction_S3.ot2.apiv2.py | 19 +++++++++++++------ 1 file changed, 13 insertions(+), 6 deletions(-) diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index 9102e4d..0f94508 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -308,6 +308,7 @@ def remove_supernatant(sources,waste,pip,tiprack): def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack): for wash_set in wash_sets: + tips_loc = 0 for i, m in enumerate(dests): # transfer and mix wash with beads magdeck.disengage() @@ -315,7 +316,7 @@ def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack): side = 1 if i % 2 == 0 else -1 asp_loc = m.bottom(1.3) disp_loc = m.bottom(5) - pick_up(pip,tiprack) + pip.pick_up() pip.transfer( 200, wash_chan.bottom(2), m.center(), new_tip='never', air_gap=20) # Mix heigh has to be really close to bottom, it was 5 now reduced to 2, maybe should be 1? @@ -334,9 +335,9 @@ def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack): pip.transfer(200, asp_loc, waste, new_tip='never', air_gap=20) pip.flow_rate.aspirate = aspire_default_speed pip.blow_out(waste) - drop(pip) + pip.drop() -def wash(wash_sets,dests,waste,magdeck,pip,tiprack): +def wash(wash_sets,dests,waste,magdeck,pip,tiprack,rip,tipreuse): for wash_set in wash_sets: for i, m in enumerate(dests): # transfer and mix wash with beads @@ -455,12 +456,17 @@ def run(ctx: protocol_api.ProtocolContext): tips300 = [ robot.load_labware( 'opentrons_96_tiprack_300ul', slot, '200µl filter tiprack') - for slot in ['2', '3', '5', '6', '9','4'] + for slot in ['2', '3', '4', '5', '6'] + ] + tipsreuse = [ + robot.load_labware( + 'opentrons_96_tiprack_300ul', slot, '200µl filter tiprack') + for slot in ['8'] ] tips1000 = [ robot.load_labware('opentrons_96_filtertiprack_1000ul', slot, '1000µl filter tiprack') - for slot in ['8'] + for slot in ['9'] ] # reagents and samples @@ -474,6 +480,7 @@ def run(ctx: protocol_api.ProtocolContext): # pipettes m300 = robot.load_instrument('p300_multi_gen2', 'left', tip_racks=tips300) + r300 = robot.load_instrument('p300_multi_gen2', 'left', tip_racks=tipsreuse) p1000 = robot.load_instrument('p1000_single_gen2', 'right', tip_racks=tips1000) @@ -518,7 +525,7 @@ def run(ctx: protocol_api.ProtocolContext): # 3x washes if REUSE_TIPS == True: - wash_reuse(wash_sets,mag_samples_m,waste,magdeck,m300,tips300) + wash_reuse(wash_sets,mag_samples_m,waste,magdeck,m300,tips300,r300,tipsreuse) else: wash(wash_sets,mag_samples_m,waste,magdeck,m300,tips300) From da94073d11dcaf4afeeb586a1cd77dd63dfed40d Mon Sep 17 00:00:00 2001 From: luissian Date: Tue, 26 May 2020 16:14:47 +0200 Subject: [PATCH 23/95] sends rest api and dumping to error log --- .gitignore | 1 + ...stationA_protocol3_lysates_S3.ot2.apiv2.py | 50 +++++++++++++++++-- 2 files changed, 46 insertions(+), 5 deletions(-) diff --git a/.gitignore b/.gitignore index b6e4761..6894ebf 100644 --- a/.gitignore +++ b/.gitignore @@ -2,6 +2,7 @@ __pycache__/ *.py[cod] *$py.class +virtualenv # C extensions *.so diff --git a/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py index 873021a..b08fc8b 100644 --- a/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py @@ -5,6 +5,7 @@ import math import os import subprocess +import requests import json from datetime import datetime @@ -100,7 +101,27 @@ } # Function definitions -def run_info(start, end, parameters = dict()): + +def write_to_error_log (info, reason): + sub_folder_date = datetime.now().strftime("%Y_%m_%d") + folder_date = os.path.join('/data', sub_folder_date) + file_name = datetime.now().strftime("%Y_%m_%d_%H_%M_%S") + '.json' + folder_file_name = os.path.join(folder_date, file_name) + folder_error_log = os.path.join(folder_date,'error.log') + if not os.isdir(folder_date): + try: + os.makedirs(folder_date) + except: + return + try: + with open (folder_file_name , 'w') as fh: + json.dump(info, fh, indent=4) + with open(folder_error_log, 'w') as fh: + fh.write('Unable to accept the requests get error : '+ reason + '\n') + except: + return + +def run_info(start, end): info = {} hostname = subprocess.run( ['hostname'], @@ -113,10 +134,29 @@ def run_info(start, end, parameters = dict()): info["ProtocolID"] = PROTOCOL_ID info["StartRunTime"] = start info["FinishRunTime"] = end - info["parameters"] = parameters - # write json to file. This is going to be an api post. - #with open('run.json', 'w') as fp: - #json.dump(info, fp,indent=4) + info["parameters"] = { + "NUM_SAMPLES" : NUM_SAMPLES, + "LYSATE_LABWARE" : LYSATE_LABWARE, + "PLATE_LABWARE" : PLATE_LABWARE, + "VOLUME_LYSATE" : VOLUME_LYSATE, + "BEADS" : BEADS, + "LANGUAGE" : LANGUAGE, + "RESET_TIPCOUNT" : RESET_TIPCOUNT} + + headers = {'Content-type': 'application/json'} + url_https = 'https://' + URL + url_http = 'http://' + URL + try: + r = requests.post(url_https, data=json.dumps(info), headers=headers) + except: + try: + r = requests.post(url_http, data=json.dumps(info), headers=headers) + except: + write_to_error_log(info, 'Server communication error') + return + if r.status_code > 201 : + write_to_error_log(info, str(r.status_code)) + def check_door(): return gpio.read_window_switches() From bf44d9f6f6999e972b54db9cae24daa2c7392b80 Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Tue, 26 May 2020 16:19:51 +0200 Subject: [PATCH 24/95] Removed useless variants in wash() and structured wash_reuse() --- ...tionB_protocol1_extraction_S3.ot2.apiv2.py | 29 ++++++++----------- 1 file changed, 12 insertions(+), 17 deletions(-) diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index 0f94508..6758520 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -306,20 +306,19 @@ def remove_supernatant(sources,waste,pip,tiprack): pip.blow_out(waste) drop(pip) -def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack): +def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack,rip,tipreuse): for wash_set in wash_sets: - tips_loc = 0 + # transfer wash + pick_up(pip,tiprack) for i, m in enumerate(dests): - # transfer and mix wash with beads magdeck.disengage() wash_chan = wash_set[i//6] - side = 1 if i % 2 == 0 else -1 - asp_loc = m.bottom(1.3) - disp_loc = m.bottom(5) - pip.pick_up() pip.transfer( - 200, wash_chan.bottom(2), m.center(), new_tip='never', air_gap=20) - # Mix heigh has to be really close to bottom, it was 5 now reduced to 2, maybe should be 1? + 200, wash_chan.bottom(2), m.top(), new_tip='never', air_gap=20) + + # mix beads with wash + tips_loc = 0 + for i, m in enumerate(dests): dispense_default_speed = pip.flow_rate.dispense pip.flow_rate.dispense = 1500 pip.mix(7, 200, m.bottom(2)) @@ -328,24 +327,22 @@ def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack): magdeck.engage(height_from_base=MAGNET_HEIGHT) robot.delay(seconds=75, msg='Incubating on magnet for 75 seconds.') - # remove supernatant + # remove supernatant + tips_loc = 0 + for i, m in enumerate(dests): aspire_default_speed = pip.flow_rate.aspirate pip.flow_rate.aspirate = 75 asp_loc = m.bottom(1.5) pip.transfer(200, asp_loc, waste, new_tip='never', air_gap=20) pip.flow_rate.aspirate = aspire_default_speed pip.blow_out(waste) - pip.drop() -def wash(wash_sets,dests,waste,magdeck,pip,tiprack,rip,tipreuse): +def wash(wash_sets,dests,waste,magdeck,pip,tiprack): for wash_set in wash_sets: for i, m in enumerate(dests): # transfer and mix wash with beads magdeck.disengage() wash_chan = wash_set[i//6] - side = 1 if i % 2 == 0 else -1 - asp_loc = m.bottom(1.3) - disp_loc = m.bottom(5) pick_up(pip,tiprack) pip.transfer( 200, wash_chan.bottom(2), m.center(), new_tip='never', air_gap=20) @@ -389,8 +386,6 @@ def elute_samples(sources,dests,buffer,magdeck,pip,tipracks): ## Dispense elutes in pcr plate. for i, (m, e) in enumerate(zip(sources, dests)): # tranfser and mix elution buffer with beads - # side = 1 if i % 2 == 0 else -1 - # asp_loc = m.bottom(5).move(Point(x=-1*side*2)) asp_loc = m.bottom(1.5) pick_up(pip,tipracks) # transfer elution to new plate From 7010f0846a13ab13df80352fe467ecf5410dd488 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Tue, 26 May 2020 16:38:47 +0200 Subject: [PATCH 25/95] added first json and draft test script --- .../par_tests/stationA_prot1_comb1_pars.json | 9 ++ tests/run_tests.py | 102 ++++++++++++++++++ 2 files changed, 111 insertions(+) create mode 100644 tests/par_tests/stationA_prot1_comb1_pars.json create mode 100644 tests/run_tests.py diff --git a/tests/par_tests/stationA_prot1_comb1_pars.json b/tests/par_tests/stationA_prot1_comb1_pars.json new file mode 100644 index 0000000..6af4a06 --- /dev/null +++ b/tests/par_tests/stationA_prot1_comb1_pars.json @@ -0,0 +1,9 @@ +{ + "NUM_SAMPLES" : 96, + "BUFFER_LABWARE" : "opentrons plastic 30ml tubes", + "DESTINATION_LABWARE" : "opentrons plastic 2ml tubes", + "DEST_TUBE" : "2ml tubes", + "VOLUME_BUFFER" : 300, + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : False +} diff --git a/tests/run_tests.py b/tests/run_tests.py new file mode 100644 index 0000000..f22c674 --- /dev/null +++ b/tests/run_tests.py @@ -0,0 +1,102 @@ +#!/usr/bin/python +import json +import os +import argparse + +# Constants +OPENROBOTS_DELIMITATION_PARAMETERS_TAGS = ['# Parameters to adapt the protocol', + '# End Parameters to adapt the protocol'] + +def get_arguments(): + + parser = argparse.ArgumentParser(prog = 'common_mash_reference.py', description= 'Search for all mash files and find the representative reference') + + parser.add_argument('-j', '--json', dest="json", metavar="json info file", type=str, required=True, help='REQUIRED. json file with service information.') + parser.add_argument('-t', '--template', dest="json", metavar="json info file", type=str, required=True, help='REQUIRED. Protocol template.') + parser.add_argument('-o', '--output', type=str, required=False, help='Output file to save results') + + arguments = parser.parse_args() + + return arguments + +def add_parameters_in_file (in_file, out_file, parameters): + ''' + Description: + The function will get protocol template file and add the parameters to create an output file + that is stored in OPENROBOTS_OUTPUT_DIRECTORYthe Labware information used in the form to create the files + Input: + in_file # template file + out_file # output file name + parameters # dictionnary with the information to include in the file + Constans: + OPENROBOTS_DELIMITATION_PARAMETERS_TAGS + Return: + form_data + ''' + if not os.path.exists(in_file): + return 'Protocol Template does not exists' + + with open (in_file, 'r') as in_fh: + found_start = False + delimitation_end_found = False + parameters_added = True + with open(out_file, 'w') as out_fh: + for line in in_fh: + parameter_section = re.search(rf'^{OPENROBOTS_DELIMITATION_PARAMETERS_TAGS[0]}', line) + end_parameter_section = re.search(rf'^{OPENROBOTS_DELIMITATION_PARAMETERS_TAGS[1]}', line) + if not found_start: + out_fh.write(line) + if parameter_section : + found_start = True + + for key in sorted(parameters): + type_of_data = get_type_of_data(parameters[key]) + if type_of_data == 'boolean' or type_of_data == 'integer': + + out_fh.write(key + ' = '+ parameters[key]+ '\n') + else: + out_fh.write(key + ' = \''+ parameters[key]+ '\'\n') + parameters_added = True + continue + if end_parameter_section : + out_fh.write(line) + found_start = False + delimitation_end_found = True + + if parameters_added and delimitation_end_found : + return 'True' + return 'Unable to write the parameters in the protocol file' + +def get_type_of_data (data): + ''' + Description: + The function get always as input a string class. + By trying to convert the input data to int or bolealn it will decide the type of data + If not possible to conver it returns string + Return: + type_of_data + ''' + boolean_values = ['True', 'False', 'None'] + if data in boolean_values : + return 'boolean' + try: + integer = int(data) + return 'integer' + except: + return 'string' + + +def main(): + +## get args +args = get_arguments() + +## load json +with open(args.json, 'r') as json: + try: + parameters=json.load(json) + except ValueError as e: + logger.error("json decoding has failed") + logger.exception(e) + +add_parameters_in_file(args.template,args.output,parameters) From ac51b19ed1b75c078d19b32c83965918a43328ed Mon Sep 17 00:00:00 2001 From: saramonzon Date: Tue, 26 May 2020 16:43:14 +0200 Subject: [PATCH 26/95] fixed some errors in run_tests.py --- tests/run_tests.py | 30 +++++++++++++++--------------- 1 file changed, 15 insertions(+), 15 deletions(-) diff --git a/tests/run_tests.py b/tests/run_tests.py index f22c674..05d52ec 100644 --- a/tests/run_tests.py +++ b/tests/run_tests.py @@ -1,6 +1,7 @@ #!/usr/bin/python import json import os +import re import argparse # Constants @@ -9,9 +10,9 @@ def get_arguments(): - parser = argparse.ArgumentParser(prog = 'common_mash_reference.py', description= 'Search for all mash files and find the representative reference') + parser = argparse.ArgumentParser(prog = 'run_tests.py', description= 'run opentrons_covid repo tests') - parser.add_argument('-j', '--json', dest="json", metavar="json info file", type=str, required=True, help='REQUIRED. json file with service information.') + parser.add_argument('-j', '--json', dest="json", metavar="json info file", type=str, required=True, help='REQUIRED. json file with parameters.') parser.add_argument('-t', '--template', dest="json", metavar="json info file", type=str, required=True, help='REQUIRED. Protocol template.') parser.add_argument('-o', '--output', type=str, required=False, help='Output file to save results') @@ -87,16 +88,15 @@ def get_type_of_data (data): def main(): - -## get args -args = get_arguments() - -## load json -with open(args.json, 'r') as json: - try: - parameters=json.load(json) - except ValueError as e: - logger.error("json decoding has failed") - logger.exception(e) - -add_parameters_in_file(args.template,args.output,parameters) + ## get args + args = get_arguments() + + ## load json + with open(args.json, 'r') as json: + try: + parameters=json.load(json) + except ValueError as e: + logger.error("json decoding has failed") + logger.exception(e) + + add_parameters_in_file(args.template,args.output,parameters) From 911827de57f230bc81232530c300da8481721c3f Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Tue, 26 May 2020 16:55:50 +0200 Subject: [PATCH 27/95] Ready to simulate and check if "tips_loc += 1" or "tips_loc += 8" --- ...tionB_protocol1_extraction_S3.ot2.apiv2.py | 21 ++++++++++++++----- 1 file changed, 16 insertions(+), 5 deletions(-) diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index 6758520..2774190 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -306,7 +306,7 @@ def remove_supernatant(sources,waste,pip,tiprack): pip.blow_out(waste) drop(pip) -def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack,rip,tipreuse): +def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack,tipreuse): for wash_set in wash_sets: # transfer wash pick_up(pip,tiprack) @@ -319,23 +319,35 @@ def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack,rip,tipreuse): # mix beads with wash tips_loc = 0 for i, m in enumerate(dests): + if tips_loc == 0: + if wash_set != 0: + drop(pip) + pip.pick_up_tip(tipreuse[0].rows()[0][tips_loc]) + else: + pip.pick_up_tip(tipreuse[0].rows()[0][tips_loc]) + dispense_default_speed = pip.flow_rate.dispense pip.flow_rate.dispense = 1500 pip.mix(7, 200, m.bottom(2)) pip.flow_rate.dispense = dispense_default_speed + pip.return_tip(tipreuse[0].rows()[0][tips_loc], home_after=False) + tips_loc += 1 - magdeck.engage(height_from_base=MAGNET_HEIGHT) - robot.delay(seconds=75, msg='Incubating on magnet for 75 seconds.') + magdeck.engage(height_from_base=MAGNET_HEIGHT) + robot.delay(seconds=75, msg='Incubating on magnet for 75 seconds.') # remove supernatant tips_loc = 0 for i, m in enumerate(dests): + pip.pick_up_tip(tipreuse[0].rows()[0][tips_loc]) aspire_default_speed = pip.flow_rate.aspirate pip.flow_rate.aspirate = 75 asp_loc = m.bottom(1.5) pip.transfer(200, asp_loc, waste, new_tip='never', air_gap=20) pip.flow_rate.aspirate = aspire_default_speed pip.blow_out(waste) + pip.return_tip(tipreuse[0].rows()[0][tips_loc], home_after=False) + tips_loc += 1 def wash(wash_sets,dests,waste,magdeck,pip,tiprack): for wash_set in wash_sets: @@ -475,7 +487,6 @@ def run(ctx: protocol_api.ProtocolContext): # pipettes m300 = robot.load_instrument('p300_multi_gen2', 'left', tip_racks=tips300) - r300 = robot.load_instrument('p300_multi_gen2', 'left', tip_racks=tipsreuse) p1000 = robot.load_instrument('p1000_single_gen2', 'right', tip_racks=tips1000) @@ -520,7 +531,7 @@ def run(ctx: protocol_api.ProtocolContext): # 3x washes if REUSE_TIPS == True: - wash_reuse(wash_sets,mag_samples_m,waste,magdeck,m300,tips300,r300,tipsreuse) + wash_reuse(wash_sets,mag_samples_m,waste,magdeck,m300,tips300,tipsreuse) else: wash(wash_sets,mag_samples_m,waste,magdeck,m300,tips300) From 0c8cc5bb49d215225b127f9d17799e7742ccf6f5 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Tue, 26 May 2020 17:14:50 +0200 Subject: [PATCH 28/95] fixed errors in run_tests.py script --- .../par_tests/stationA_prot1_comb1_pars.json | 2 +- tests/run_tests.py | 32 +++++++++++-------- 2 files changed, 19 insertions(+), 15 deletions(-) diff --git a/tests/par_tests/stationA_prot1_comb1_pars.json b/tests/par_tests/stationA_prot1_comb1_pars.json index 6af4a06..56c9a5a 100644 --- a/tests/par_tests/stationA_prot1_comb1_pars.json +++ b/tests/par_tests/stationA_prot1_comb1_pars.json @@ -5,5 +5,5 @@ "DEST_TUBE" : "2ml tubes", "VOLUME_BUFFER" : 300, "LANGUAGE" : "esp", - "RESET_TIPCOUNT" : False + "RESET_TIPCOUNT" : "False" } diff --git a/tests/run_tests.py b/tests/run_tests.py index 05d52ec..105f080 100644 --- a/tests/run_tests.py +++ b/tests/run_tests.py @@ -9,16 +9,15 @@ '# End Parameters to adapt the protocol'] def get_arguments(): + parser = argparse.ArgumentParser(prog = 'run_tests.py', description= 'run opentrons_covid repo tests') - parser = argparse.ArgumentParser(prog = 'run_tests.py', description= 'run opentrons_covid repo tests') + parser.add_argument('-j', '--json', dest="json", metavar="json info file", type=str, required=True, help='REQUIRED. json file with parameters.') + parser.add_argument('-t', '--template', dest="template", metavar="json info file", type=str, required=True, help='REQUIRED. Protocol template.') + parser.add_argument('-o', '--output', type=str, required=False, help='Output file to save results') - parser.add_argument('-j', '--json', dest="json", metavar="json info file", type=str, required=True, help='REQUIRED. json file with parameters.') - parser.add_argument('-t', '--template', dest="json", metavar="json info file", type=str, required=True, help='REQUIRED. Protocol template.') - parser.add_argument('-o', '--output', type=str, required=False, help='Output file to save results') + arguments = parser.parse_args() - arguments = parser.parse_args() - - return arguments + return arguments def add_parameters_in_file (in_file, out_file, parameters): ''' @@ -53,8 +52,7 @@ def add_parameters_in_file (in_file, out_file, parameters): for key in sorted(parameters): type_of_data = get_type_of_data(parameters[key]) if type_of_data == 'boolean' or type_of_data == 'integer': - - out_fh.write(key + ' = '+ parameters[key]+ '\n') + out_fh.write(key + ' = '+ str(parameters[key]) + '\n') else: out_fh.write(key + ' = \''+ parameters[key]+ '\'\n') parameters_added = True @@ -86,17 +84,23 @@ def get_type_of_data (data): except: return 'string' - def main(): ## get args args = get_arguments() + print(args) ## load json - with open(args.json, 'r') as json: + with open(args.json, 'r') as file: try: - parameters=json.load(json) + parameters=json.load(file) except ValueError as e: - logger.error("json decoding has failed") - logger.exception(e) + print("json decoding has failed") + print(e) add_parameters_in_file(args.template,args.output,parameters) + +if __name__ == '__main__': + try: + main() + except Exception as e: + print(e) From 0816f759b818227e5950ae1cd29e0e5fbc8fc47d Mon Sep 17 00:00:00 2001 From: saramonzon Date: Tue, 26 May 2020 17:30:54 +0200 Subject: [PATCH 29/95] added first try github actions for testing --- .github/tests.yml | 25 +++++++++++++++++++++++++ 1 file changed, 25 insertions(+) create mode 100644 .github/tests.yml diff --git a/.github/tests.yml b/.github/tests.yml new file mode 100644 index 0000000..ee0535b --- /dev/null +++ b/.github/tests.yml @@ -0,0 +1,25 @@ +name: opentrons_covid CI +# This workflow is triggered on pushes and PRs to the repository. +# It runs the pipeline with several tests to check if everything works + +jobs: + test: + env: + runs-on: ubuntu-latest + strategy: + matrix: + steps: + - uses: actions/checkout@v2 + - name: Install opentrons api + run: | + python -m pip install --upgrade pip + pip install opentrons==3.17.0 + - name: Create test script + run: | + python ${GITHUB_WORKSPACE}/tests/run_tests.py \ + -j ${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot1_comb1_pars.json \ + -t ${GITHUB_WORKSPACE}/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py \ + -o /tmp/tmp_test.py + - name: Simulate script + run: | + opentrons_simulate -L ${GITHUB_WORKSPACE}/labware /tmp/tmp_test.py From 4c66e6c1f2414298737a59997319f5f2ce57dbb4 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Tue, 26 May 2020 17:33:04 +0200 Subject: [PATCH 30/95] move test.yml to correct folder --- .github/{ => workflows}/tests.yml | 0 1 file changed, 0 insertions(+), 0 deletions(-) rename .github/{ => workflows}/tests.yml (100%) diff --git a/.github/tests.yml b/.github/workflows/tests.yml similarity index 100% rename from .github/tests.yml rename to .github/workflows/tests.yml From e53add0d6cea88073495b4f98892b7999cd05b13 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Tue, 26 May 2020 17:36:03 +0200 Subject: [PATCH 31/95] all in one line test.yml --- .github/workflows/tests.yml | 5 +---- 1 file changed, 1 insertion(+), 4 deletions(-) diff --git a/.github/workflows/tests.yml b/.github/workflows/tests.yml index ee0535b..0df1615 100644 --- a/.github/workflows/tests.yml +++ b/.github/workflows/tests.yml @@ -16,10 +16,7 @@ jobs: pip install opentrons==3.17.0 - name: Create test script run: | - python ${GITHUB_WORKSPACE}/tests/run_tests.py \ - -j ${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot1_comb1_pars.json \ - -t ${GITHUB_WORKSPACE}/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py \ - -o /tmp/tmp_test.py + python ${GITHUB_WORKSPACE}/tests/run_tests.py -j ${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot1_comb1_pars.json -t ${GITHUB_WORKSPACE}/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py -o /tmp/tmp_test.py - name: Simulate script run: | opentrons_simulate -L ${GITHUB_WORKSPACE}/labware /tmp/tmp_test.py From 0d61387932255cb903a66c3cbd4e47d2c2dd5949 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Tue, 26 May 2020 17:41:54 +0200 Subject: [PATCH 32/95] try to fix stuff in tests --- .github/workflows/tests.yml | 6 +++++- 1 file changed, 5 insertions(+), 1 deletion(-) diff --git a/.github/workflows/tests.yml b/.github/workflows/tests.yml index 0df1615..e139383 100644 --- a/.github/workflows/tests.yml +++ b/.github/workflows/tests.yml @@ -10,7 +10,11 @@ jobs: matrix: steps: - uses: actions/checkout@v2 - - name: Install opentrons api + - name: Set up Python ${{ matrix.python-version }} + uses: actions/setup-python@v1 + with: + python-version: 3.7 + - name: Install Dependencies run: | python -m pip install --upgrade pip pip install opentrons==3.17.0 From f57c5e1a3a68d8f9e88561e2bc6e81ae2edae9f3 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Tue, 26 May 2020 17:43:46 +0200 Subject: [PATCH 33/95] i don't know what is wrong... --- .github/workflows/tests.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/tests.yml b/.github/workflows/tests.yml index e139383..b44fb9f 100644 --- a/.github/workflows/tests.yml +++ b/.github/workflows/tests.yml @@ -17,7 +17,7 @@ jobs: - name: Install Dependencies run: | python -m pip install --upgrade pip - pip install opentrons==3.17.0 + pip install opentrons - name: Create test script run: | python ${GITHUB_WORKSPACE}/tests/run_tests.py -j ${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot1_comb1_pars.json -t ${GITHUB_WORKSPACE}/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py -o /tmp/tmp_test.py From 42eb9e2e028e83a5335191456f3967f997e2df31 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Tue, 26 May 2020 17:45:25 +0200 Subject: [PATCH 34/95] test del test --- .github/workflows/test_del_test.yml | 30 +++++++++++++++++++++++++++++ 1 file changed, 30 insertions(+) create mode 100644 .github/workflows/test_del_test.yml diff --git a/.github/workflows/test_del_test.yml b/.github/workflows/test_del_test.yml new file mode 100644 index 0000000..c806047 --- /dev/null +++ b/.github/workflows/test_del_test.yml @@ -0,0 +1,30 @@ +name: Django CI + +on: + push: + branches: [ master ] + pull_request: + branches: [ master ] + +jobs: + build: + + runs-on: ubuntu-latest + strategy: + max-parallel: 4 + matrix: + python-version: [3.6, 3.7, 3.8] + + steps: + - uses: actions/checkout@v2 + - name: Set up Python ${{ matrix.python-version }} + uses: actions/setup-python@v1 + with: + python-version: ${{ matrix.python-version }} + - name: Install Dependencies + run: | + python -m pip install --upgrade pip + pip install -r requirements.txt + - name: Run Tests + run: | + python manage.py test From a9cc852ee50bba2ff01fd80bb59c0dca36e2ab20 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Tue, 26 May 2020 17:46:55 +0200 Subject: [PATCH 35/95] keep testing... --- .github/workflows/test_del_test.yml | 6 +----- .github/workflows/tests.yml | 2 +- 2 files changed, 2 insertions(+), 6 deletions(-) diff --git a/.github/workflows/test_del_test.yml b/.github/workflows/test_del_test.yml index c806047..d860c86 100644 --- a/.github/workflows/test_del_test.yml +++ b/.github/workflows/test_del_test.yml @@ -1,10 +1,6 @@ name: Django CI -on: - push: - branches: [ master ] - pull_request: - branches: [ master ] +on: [push, pull_request] jobs: build: diff --git a/.github/workflows/tests.yml b/.github/workflows/tests.yml index b44fb9f..be0772f 100644 --- a/.github/workflows/tests.yml +++ b/.github/workflows/tests.yml @@ -1,7 +1,7 @@ name: opentrons_covid CI # This workflow is triggered on pushes and PRs to the repository. # It runs the pipeline with several tests to check if everything works - +on: [push, pull_request] jobs: test: env: From a94e04363891490fe453f4aee29c5dfb664a741b Mon Sep 17 00:00:00 2001 From: saramonzon Date: Tue, 26 May 2020 17:49:48 +0200 Subject: [PATCH 36/95] more testing --- .github/workflows/tests.yml | 6 ++---- 1 file changed, 2 insertions(+), 4 deletions(-) diff --git a/.github/workflows/tests.yml b/.github/workflows/tests.yml index be0772f..90209f2 100644 --- a/.github/workflows/tests.yml +++ b/.github/workflows/tests.yml @@ -4,10 +4,7 @@ name: opentrons_covid CI on: [push, pull_request] jobs: test: - env: runs-on: ubuntu-latest - strategy: - matrix: steps: - uses: actions/checkout@v2 - name: Set up Python ${{ matrix.python-version }} @@ -20,7 +17,8 @@ jobs: pip install opentrons - name: Create test script run: | - python ${GITHUB_WORKSPACE}/tests/run_tests.py -j ${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot1_comb1_pars.json -t ${GITHUB_WORKSPACE}/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py -o /tmp/tmp_test.py + echo "testing" + #python ${GITHUB_WORKSPACE}/tests/run_tests.py -j ${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot1_comb1_pars.json -t ${GITHUB_WORKSPACE}/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py -o /tmp/tmp_test.py - name: Simulate script run: | opentrons_simulate -L ${GITHUB_WORKSPACE}/labware /tmp/tmp_test.py From 25923ce8132839e2534e44d6c848f28db32a0f38 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Tue, 26 May 2020 17:51:17 +0200 Subject: [PATCH 37/95] and some more... --- .github/workflows/test_del_test.yml | 4 ++++ .github/workflows/tests.yml | 4 ---- 2 files changed, 4 insertions(+), 4 deletions(-) diff --git a/.github/workflows/test_del_test.yml b/.github/workflows/test_del_test.yml index d860c86..cbfc174 100644 --- a/.github/workflows/test_del_test.yml +++ b/.github/workflows/test_del_test.yml @@ -21,6 +21,10 @@ jobs: run: | python -m pip install --upgrade pip pip install -r requirements.txt + - name: Create test script + run: | + echo "testing" + #python ${GITHUB_WORKSPACE}/tests/run_tests.py -j ${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot1_comb1_pars.json -t ${GITHUB_WORKSPACE}/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py -o /tmp/tmp_test.py - name: Run Tests run: | python manage.py test diff --git a/.github/workflows/tests.yml b/.github/workflows/tests.yml index 90209f2..57766b7 100644 --- a/.github/workflows/tests.yml +++ b/.github/workflows/tests.yml @@ -15,10 +15,6 @@ jobs: run: | python -m pip install --upgrade pip pip install opentrons - - name: Create test script - run: | - echo "testing" - #python ${GITHUB_WORKSPACE}/tests/run_tests.py -j ${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot1_comb1_pars.json -t ${GITHUB_WORKSPACE}/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py -o /tmp/tmp_test.py - name: Simulate script run: | opentrons_simulate -L ${GITHUB_WORKSPACE}/labware /tmp/tmp_test.py From d66d31c6fe5e17b2ba3ed62dda8e017c4ce27829 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Tue, 26 May 2020 17:52:41 +0200 Subject: [PATCH 38/95] i'm getting there... --- .github/workflows/test_del_test.yml | 4 ++-- .github/workflows/tests.yml | 2 +- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/.github/workflows/test_del_test.yml b/.github/workflows/test_del_test.yml index cbfc174..6518ce8 100644 --- a/.github/workflows/test_del_test.yml +++ b/.github/workflows/test_del_test.yml @@ -25,6 +25,6 @@ jobs: run: | echo "testing" #python ${GITHUB_WORKSPACE}/tests/run_tests.py -j ${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot1_comb1_pars.json -t ${GITHUB_WORKSPACE}/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py -o /tmp/tmp_test.py - - name: Run Tests + - name: Simulate script run: | - python manage.py test + opentrons_simulate -L ${GITHUB_WORKSPACE}/labware /tmp/tmp_test.py diff --git a/.github/workflows/tests.yml b/.github/workflows/tests.yml index 57766b7..82ecc84 100644 --- a/.github/workflows/tests.yml +++ b/.github/workflows/tests.yml @@ -14,7 +14,7 @@ jobs: - name: Install Dependencies run: | python -m pip install --upgrade pip - pip install opentrons + pip install -r requirements - name: Simulate script run: | opentrons_simulate -L ${GITHUB_WORKSPACE}/labware /tmp/tmp_test.py From f6a6ed9ec70ffdff749c13ef99e4f2a4171ebf6c Mon Sep 17 00:00:00 2001 From: saramonzon Date: Tue, 26 May 2020 17:54:38 +0200 Subject: [PATCH 39/95] maybe this is the good one.. --- .github/workflows/tests.yml | 8 ++++++-- 1 file changed, 6 insertions(+), 2 deletions(-) diff --git a/.github/workflows/tests.yml b/.github/workflows/tests.yml index 82ecc84..98cf90a 100644 --- a/.github/workflows/tests.yml +++ b/.github/workflows/tests.yml @@ -13,8 +13,12 @@ jobs: python-version: 3.7 - name: Install Dependencies run: | - python -m pip install --upgrade pip - pip install -r requirements + python -m pip install --upgrade pip + pip install -r requirements + - name: Create test script + run: | + echo "testing" + python ${GITHUB_WORKSPACE}/tests/run_tests.py -j ${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot1_comb1_pars.json -t ${GITHUB_WORKSPACE}/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py -o /tmp/tmp_test.py - name: Simulate script run: | opentrons_simulate -L ${GITHUB_WORKSPACE}/labware /tmp/tmp_test.py From 87fd6d6102d0a4e1aed31155a5da0b110ec35d77 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Tue, 26 May 2020 17:56:05 +0200 Subject: [PATCH 40/95] yey! stupid issue solved! Now fixed minor issue --- .github/workflows/tests.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/tests.yml b/.github/workflows/tests.yml index 98cf90a..ea49a48 100644 --- a/.github/workflows/tests.yml +++ b/.github/workflows/tests.yml @@ -14,7 +14,7 @@ jobs: - name: Install Dependencies run: | python -m pip install --upgrade pip - pip install -r requirements + pip install -r opentrons=3.17.0 - name: Create test script run: | echo "testing" From 0b9ae95537a51ca8bc8f03b89030039fa32f6b18 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Tue, 26 May 2020 17:57:16 +0200 Subject: [PATCH 41/95] now really fixed pip package name.. --- .github/workflows/tests.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/tests.yml b/.github/workflows/tests.yml index ea49a48..7f6b5ad 100644 --- a/.github/workflows/tests.yml +++ b/.github/workflows/tests.yml @@ -14,7 +14,7 @@ jobs: - name: Install Dependencies run: | python -m pip install --upgrade pip - pip install -r opentrons=3.17.0 + pip install opentrons==3.17.0 - name: Create test script run: | echo "testing" From 3ab2d6716a29b6f4fd9e80e610a1a1a0f3df5a1d Mon Sep 17 00:00:00 2001 From: saramonzon Date: Tue, 26 May 2020 17:57:43 +0200 Subject: [PATCH 42/95] deleted fake test file --- .github/workflows/test_del_test.yml | 30 ----------------------------- 1 file changed, 30 deletions(-) delete mode 100644 .github/workflows/test_del_test.yml diff --git a/.github/workflows/test_del_test.yml b/.github/workflows/test_del_test.yml deleted file mode 100644 index 6518ce8..0000000 --- a/.github/workflows/test_del_test.yml +++ /dev/null @@ -1,30 +0,0 @@ -name: Django CI - -on: [push, pull_request] - -jobs: - build: - - runs-on: ubuntu-latest - strategy: - max-parallel: 4 - matrix: - python-version: [3.6, 3.7, 3.8] - - steps: - - uses: actions/checkout@v2 - - name: Set up Python ${{ matrix.python-version }} - uses: actions/setup-python@v1 - with: - python-version: ${{ matrix.python-version }} - - name: Install Dependencies - run: | - python -m pip install --upgrade pip - pip install -r requirements.txt - - name: Create test script - run: | - echo "testing" - #python ${GITHUB_WORKSPACE}/tests/run_tests.py -j ${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot1_comb1_pars.json -t ${GITHUB_WORKSPACE}/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py -o /tmp/tmp_test.py - - name: Simulate script - run: | - opentrons_simulate -L ${GITHUB_WORKSPACE}/labware /tmp/tmp_test.py From 14fc6484226fca3a19fcfab204d1d6b9af19b09b Mon Sep 17 00:00:00 2001 From: saramonzon Date: Tue, 26 May 2020 17:59:36 +0200 Subject: [PATCH 43/95] I think i almost have it! Added protocol_id variable for testing pars --- tests/par_tests/stationA_prot1_comb1_pars.json | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/tests/par_tests/stationA_prot1_comb1_pars.json b/tests/par_tests/stationA_prot1_comb1_pars.json index 56c9a5a..ba77650 100644 --- a/tests/par_tests/stationA_prot1_comb1_pars.json +++ b/tests/par_tests/stationA_prot1_comb1_pars.json @@ -5,5 +5,6 @@ "DEST_TUBE" : "2ml tubes", "VOLUME_BUFFER" : 300, "LANGUAGE" : "esp", - "RESET_TIPCOUNT" : "False" + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-test" } From 4885a561678f588b6fd8fac9138bec2cf4898f89 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Tue, 26 May 2020 18:12:18 +0200 Subject: [PATCH 44/95] added templates for new tests --- tests/par_tests/stationA_prot1_comb2_pars.json | 10 ++++++++++ tests/par_tests/stationA_prot1_comb3_pars.json | 10 ++++++++++ tests/par_tests/stationA_prot2_comb1_pars.json | 10 ++++++++++ tests/par_tests/stationA_prot2_comb2_pars.json | 10 ++++++++++ tests/par_tests/stationA_prot2_comb3_pars.json | 10 ++++++++++ tests/par_tests/stationA_prot3_comb1_pars.json | 10 ++++++++++ tests/par_tests/stationA_prot3_comb2_pars.json | 10 ++++++++++ tests/par_tests/stationA_prot3_comb3_pars.json | 10 ++++++++++ tests/par_tests/stationB_prot1_comb1_pars.json | 0 tests/par_tests/stationB_prot1_comb2_pars.json | 0 tests/par_tests/stationB_prot1_comb3_pars.json | 0 tests/par_tests/stationC_prot1_comb1_pars.json | 0 tests/par_tests/stationC_prot1_comb2_pars.json | 0 tests/par_tests/stationC_prot1_comb3_pars.json | 0 tests/par_tests/stationC_prot2_comb1pars.json | 0 tests/par_tests/stationC_prot2_comb2_pars.json | 0 tests/par_tests/stationC_prot2_comb3_pars.json | 0 17 files changed, 80 insertions(+) create mode 100644 tests/par_tests/stationA_prot1_comb2_pars.json create mode 100644 tests/par_tests/stationA_prot1_comb3_pars.json create mode 100644 tests/par_tests/stationA_prot2_comb1_pars.json create mode 100644 tests/par_tests/stationA_prot2_comb2_pars.json create mode 100644 tests/par_tests/stationA_prot2_comb3_pars.json create mode 100644 tests/par_tests/stationA_prot3_comb1_pars.json create mode 100644 tests/par_tests/stationA_prot3_comb2_pars.json create mode 100644 tests/par_tests/stationA_prot3_comb3_pars.json create mode 100644 tests/par_tests/stationB_prot1_comb1_pars.json create mode 100644 tests/par_tests/stationB_prot1_comb2_pars.json create mode 100644 tests/par_tests/stationB_prot1_comb3_pars.json create mode 100644 tests/par_tests/stationC_prot1_comb1_pars.json create mode 100644 tests/par_tests/stationC_prot1_comb2_pars.json create mode 100644 tests/par_tests/stationC_prot1_comb3_pars.json create mode 100644 tests/par_tests/stationC_prot2_comb1pars.json create mode 100644 tests/par_tests/stationC_prot2_comb2_pars.json create mode 100644 tests/par_tests/stationC_prot2_comb3_pars.json diff --git a/tests/par_tests/stationA_prot1_comb2_pars.json b/tests/par_tests/stationA_prot1_comb2_pars.json new file mode 100644 index 0000000..ba77650 --- /dev/null +++ b/tests/par_tests/stationA_prot1_comb2_pars.json @@ -0,0 +1,10 @@ +{ + "NUM_SAMPLES" : 96, + "BUFFER_LABWARE" : "opentrons plastic 30ml tubes", + "DESTINATION_LABWARE" : "opentrons plastic 2ml tubes", + "DEST_TUBE" : "2ml tubes", + "VOLUME_BUFFER" : 300, + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-test" +} diff --git a/tests/par_tests/stationA_prot1_comb3_pars.json b/tests/par_tests/stationA_prot1_comb3_pars.json new file mode 100644 index 0000000..ba77650 --- /dev/null +++ b/tests/par_tests/stationA_prot1_comb3_pars.json @@ -0,0 +1,10 @@ +{ + "NUM_SAMPLES" : 96, + "BUFFER_LABWARE" : "opentrons plastic 30ml tubes", + "DESTINATION_LABWARE" : "opentrons plastic 2ml tubes", + "DEST_TUBE" : "2ml tubes", + "VOLUME_BUFFER" : 300, + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-test" +} diff --git a/tests/par_tests/stationA_prot2_comb1_pars.json b/tests/par_tests/stationA_prot2_comb1_pars.json new file mode 100644 index 0000000..ba77650 --- /dev/null +++ b/tests/par_tests/stationA_prot2_comb1_pars.json @@ -0,0 +1,10 @@ +{ + "NUM_SAMPLES" : 96, + "BUFFER_LABWARE" : "opentrons plastic 30ml tubes", + "DESTINATION_LABWARE" : "opentrons plastic 2ml tubes", + "DEST_TUBE" : "2ml tubes", + "VOLUME_BUFFER" : 300, + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-test" +} diff --git a/tests/par_tests/stationA_prot2_comb2_pars.json b/tests/par_tests/stationA_prot2_comb2_pars.json new file mode 100644 index 0000000..ba77650 --- /dev/null +++ b/tests/par_tests/stationA_prot2_comb2_pars.json @@ -0,0 +1,10 @@ +{ + "NUM_SAMPLES" : 96, + "BUFFER_LABWARE" : "opentrons plastic 30ml tubes", + "DESTINATION_LABWARE" : "opentrons plastic 2ml tubes", + "DEST_TUBE" : "2ml tubes", + "VOLUME_BUFFER" : 300, + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-test" +} diff --git a/tests/par_tests/stationA_prot2_comb3_pars.json b/tests/par_tests/stationA_prot2_comb3_pars.json new file mode 100644 index 0000000..ba77650 --- /dev/null +++ b/tests/par_tests/stationA_prot2_comb3_pars.json @@ -0,0 +1,10 @@ +{ + "NUM_SAMPLES" : 96, + "BUFFER_LABWARE" : "opentrons plastic 30ml tubes", + "DESTINATION_LABWARE" : "opentrons plastic 2ml tubes", + "DEST_TUBE" : "2ml tubes", + "VOLUME_BUFFER" : 300, + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-test" +} diff --git a/tests/par_tests/stationA_prot3_comb1_pars.json b/tests/par_tests/stationA_prot3_comb1_pars.json new file mode 100644 index 0000000..ba77650 --- /dev/null +++ b/tests/par_tests/stationA_prot3_comb1_pars.json @@ -0,0 +1,10 @@ +{ + "NUM_SAMPLES" : 96, + "BUFFER_LABWARE" : "opentrons plastic 30ml tubes", + "DESTINATION_LABWARE" : "opentrons plastic 2ml tubes", + "DEST_TUBE" : "2ml tubes", + "VOLUME_BUFFER" : 300, + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-test" +} diff --git a/tests/par_tests/stationA_prot3_comb2_pars.json b/tests/par_tests/stationA_prot3_comb2_pars.json new file mode 100644 index 0000000..ba77650 --- /dev/null +++ b/tests/par_tests/stationA_prot3_comb2_pars.json @@ -0,0 +1,10 @@ +{ + "NUM_SAMPLES" : 96, + "BUFFER_LABWARE" : "opentrons plastic 30ml tubes", + "DESTINATION_LABWARE" : "opentrons plastic 2ml tubes", + "DEST_TUBE" : "2ml tubes", + "VOLUME_BUFFER" : 300, + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-test" +} diff --git a/tests/par_tests/stationA_prot3_comb3_pars.json b/tests/par_tests/stationA_prot3_comb3_pars.json new file mode 100644 index 0000000..ba77650 --- /dev/null +++ b/tests/par_tests/stationA_prot3_comb3_pars.json @@ -0,0 +1,10 @@ +{ + "NUM_SAMPLES" : 96, + "BUFFER_LABWARE" : "opentrons plastic 30ml tubes", + "DESTINATION_LABWARE" : "opentrons plastic 2ml tubes", + "DEST_TUBE" : "2ml tubes", + "VOLUME_BUFFER" : 300, + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-test" +} diff --git a/tests/par_tests/stationB_prot1_comb1_pars.json b/tests/par_tests/stationB_prot1_comb1_pars.json new file mode 100644 index 0000000..e69de29 diff --git a/tests/par_tests/stationB_prot1_comb2_pars.json b/tests/par_tests/stationB_prot1_comb2_pars.json new file mode 100644 index 0000000..e69de29 diff --git a/tests/par_tests/stationB_prot1_comb3_pars.json b/tests/par_tests/stationB_prot1_comb3_pars.json new file mode 100644 index 0000000..e69de29 diff --git a/tests/par_tests/stationC_prot1_comb1_pars.json b/tests/par_tests/stationC_prot1_comb1_pars.json new file mode 100644 index 0000000..e69de29 diff --git a/tests/par_tests/stationC_prot1_comb2_pars.json b/tests/par_tests/stationC_prot1_comb2_pars.json new file mode 100644 index 0000000..e69de29 diff --git a/tests/par_tests/stationC_prot1_comb3_pars.json b/tests/par_tests/stationC_prot1_comb3_pars.json new file mode 100644 index 0000000..e69de29 diff --git a/tests/par_tests/stationC_prot2_comb1pars.json b/tests/par_tests/stationC_prot2_comb1pars.json new file mode 100644 index 0000000..e69de29 diff --git a/tests/par_tests/stationC_prot2_comb2_pars.json b/tests/par_tests/stationC_prot2_comb2_pars.json new file mode 100644 index 0000000..e69de29 diff --git a/tests/par_tests/stationC_prot2_comb3_pars.json b/tests/par_tests/stationC_prot2_comb3_pars.json new file mode 100644 index 0000000..e69de29 From a51c81abb1062b260586709eac54cb0c65a0ce92 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Tue, 26 May 2020 18:13:03 +0200 Subject: [PATCH 45/95] try several lines in yml file --- .github/workflows/tests.yml | 5 ++++- 1 file changed, 4 insertions(+), 1 deletion(-) diff --git a/.github/workflows/tests.yml b/.github/workflows/tests.yml index 7f6b5ad..05e8082 100644 --- a/.github/workflows/tests.yml +++ b/.github/workflows/tests.yml @@ -18,7 +18,10 @@ jobs: - name: Create test script run: | echo "testing" - python ${GITHUB_WORKSPACE}/tests/run_tests.py -j ${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot1_comb1_pars.json -t ${GITHUB_WORKSPACE}/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py -o /tmp/tmp_test.py + python ${GITHUB_WORKSPACE}/tests/run_tests.py \ + -j ${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot1_comb1_pars.json \ + -t ${GITHUB_WORKSPACE}/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py \ + -o /tmp/tmp_test.py - name: Simulate script run: | opentrons_simulate -L ${GITHUB_WORKSPACE}/labware /tmp/tmp_test.py From d3a501544bc543569ef6bba298b10ba407d0d6e5 Mon Sep 17 00:00:00 2001 From: luissian Date: Tue, 26 May 2020 19:34:39 +0200 Subject: [PATCH 46/95] fixing bugs when saving error log --- ...stationA_protocol3_lysates_S3.ot2.apiv2.py | 26 +++++++------------ 1 file changed, 9 insertions(+), 17 deletions(-) diff --git a/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py index b08fc8b..acf82d3 100644 --- a/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py @@ -103,12 +103,13 @@ # Function definitions def write_to_error_log (info, reason): - sub_folder_date = datetime.now().strftime("%Y_%m_%d") - folder_date = os.path.join('/data', sub_folder_date) - file_name = datetime.now().strftime("%Y_%m_%d_%H_%M_%S") + '.json' - folder_file_name = os.path.join(folder_date, file_name) + date = datetime.now().strftime("%Y_%m_%d") + folder_date = os.path.join('/data', date) + time_now = datetime.now().strftime("%Y_%m_%d_%H_%M_%S") + json_file = time_now + '.json' + folder_file_name = os.path.join(folder_date, json_file) folder_error_log = os.path.join(folder_date,'error.log') - if not os.isdir(folder_date): + if not os.path.exists(folder_date): try: os.makedirs(folder_date) except: @@ -116,8 +117,8 @@ def write_to_error_log (info, reason): try: with open (folder_file_name , 'w') as fh: json.dump(info, fh, indent=4) - with open(folder_error_log, 'w') as fh: - fh.write('Unable to accept the requests get error : '+ reason + '\n') + with open(folder_error_log, 'a') as fh: + fh.write( time_now + ' Unable to accept the requests get error : '+ reason + '\n') except: return @@ -360,13 +361,4 @@ def run(ctx: protocol_api.ProtocolContext): finish_time = finish_run() - par = { - "NUM_SAMPLES" : NUM_SAMPLES, - "LYSATE_LABWARE" : LYSATE_LABWARE, - "PLATE_LABWARE" : PLATE_LABWARE, - "VOLUME_LYSATE" : VOLUME_LYSATE, - "BEADS" : BEADS, - "LANGUAGE" : LANGUAGE, - "RESET_TIPCOUNT" : RESET_TIPCOUNT - } - run_info(start_time, finish_time, par) + run_info(start_time, finish_time) From 26cb73309a74bb08ab95355b1006d5971a2cfa76 Mon Sep 17 00:00:00 2001 From: luissian Date: Wed, 27 May 2020 10:47:52 +0200 Subject: [PATCH 47/95] moving parameters outside function --- ...stationA_protocol3_lysates_S3.ot2.apiv2.py | 22 ++++++++++--------- 1 file changed, 12 insertions(+), 10 deletions(-) diff --git a/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py index acf82d3..828266a 100644 --- a/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py @@ -122,7 +122,7 @@ def write_to_error_log (info, reason): except: return -def run_info(start, end): +def run_info(start, end, parameters = dict()): info = {} hostname = subprocess.run( ['hostname'], @@ -135,14 +135,7 @@ def run_info(start, end): info["ProtocolID"] = PROTOCOL_ID info["StartRunTime"] = start info["FinishRunTime"] = end - info["parameters"] = { - "NUM_SAMPLES" : NUM_SAMPLES, - "LYSATE_LABWARE" : LYSATE_LABWARE, - "PLATE_LABWARE" : PLATE_LABWARE, - "VOLUME_LYSATE" : VOLUME_LYSATE, - "BEADS" : BEADS, - "LANGUAGE" : LANGUAGE, - "RESET_TIPCOUNT" : RESET_TIPCOUNT} + info["parameters"] = parameters headers = {'Content-type': 'application/json'} url_https = 'https://' + URL @@ -361,4 +354,13 @@ def run(ctx: protocol_api.ProtocolContext): finish_time = finish_run() - run_info(start_time, finish_time) + parameters = { + "NUM_SAMPLES" : NUM_SAMPLES, + "LYSATE_LABWARE" : LYSATE_LABWARE, + "PLATE_LABWARE" : PLATE_LABWARE, + "VOLUME_LYSATE" : VOLUME_LYSATE, + "BEADS" : BEADS, + "LANGUAGE" : LANGUAGE, + "RESET_TIPCOUNT" : RESET_TIPCOUNT} + + run_info(start_time, finish_time, parameters) From 54ab8d99485cff1f40153fbe941e22cc5baa6b86 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Wed, 27 May 2020 11:24:19 +0200 Subject: [PATCH 48/95] create json tests for protocols in station A --- tests/par_tests/stationA_prot1_comb2_pars.json | 4 ++-- tests/par_tests/stationA_prot1_comb3_pars.json | 2 +- tests/par_tests/stationA_prot1_comb4_pars.json | 10 ++++++++++ tests/par_tests/stationA_prot2_comb1_pars.json | 10 +++++----- tests/par_tests/stationA_prot2_comb2_pars.json | 10 +++++----- tests/par_tests/stationA_prot2_comb3_pars.json | 10 +++++----- tests/par_tests/stationA_prot2_comb4_pars.json | 10 ++++++++++ tests/par_tests/stationA_prot2_comb5_pars.json | 10 ++++++++++ tests/par_tests/stationA_prot2_comb6_pars.json | 10 ++++++++++ tests/par_tests/stationA_prot2_comb7_pars.json | 10 ++++++++++ tests/par_tests/stationA_prot3_comb1_pars.json | 10 +++++----- tests/par_tests/stationA_prot3_comb2_pars.json | 10 +++++----- tests/par_tests/stationA_prot3_comb3_pars.json | 10 +++++----- tests/par_tests/stationA_prot3_comb4_pars.json | 10 ++++++++++ tests/par_tests/stationA_prot3_comb5_pars.json | 10 ++++++++++ tests/par_tests/stationA_prot3_comb6_pars.json | 10 ++++++++++ tests/par_tests/stationA_prot3_comb7_pars.json | 10 ++++++++++ tests/par_tests/stationA_prot3_comb8_pars.json | 10 ++++++++++ 18 files changed, 133 insertions(+), 33 deletions(-) create mode 100644 tests/par_tests/stationA_prot1_comb4_pars.json create mode 100644 tests/par_tests/stationA_prot2_comb4_pars.json create mode 100644 tests/par_tests/stationA_prot2_comb5_pars.json create mode 100644 tests/par_tests/stationA_prot2_comb6_pars.json create mode 100644 tests/par_tests/stationA_prot2_comb7_pars.json create mode 100644 tests/par_tests/stationA_prot3_comb4_pars.json create mode 100644 tests/par_tests/stationA_prot3_comb5_pars.json create mode 100644 tests/par_tests/stationA_prot3_comb6_pars.json create mode 100644 tests/par_tests/stationA_prot3_comb7_pars.json create mode 100644 tests/par_tests/stationA_prot3_comb8_pars.json diff --git a/tests/par_tests/stationA_prot1_comb2_pars.json b/tests/par_tests/stationA_prot1_comb2_pars.json index ba77650..ca0d165 100644 --- a/tests/par_tests/stationA_prot1_comb2_pars.json +++ b/tests/par_tests/stationA_prot1_comb2_pars.json @@ -5,6 +5,6 @@ "DEST_TUBE" : "2ml tubes", "VOLUME_BUFFER" : 300, "LANGUAGE" : "esp", - "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-test" + "RESET_TIPCOUNT" : "True", + "PROTOCOL_ID" : "0000-test" } diff --git a/tests/par_tests/stationA_prot1_comb3_pars.json b/tests/par_tests/stationA_prot1_comb3_pars.json index ba77650..9df4d0b 100644 --- a/tests/par_tests/stationA_prot1_comb3_pars.json +++ b/tests/par_tests/stationA_prot1_comb3_pars.json @@ -1,6 +1,6 @@ { "NUM_SAMPLES" : 96, - "BUFFER_LABWARE" : "opentrons plastic 30ml tubes", + "BUFFER_LABWARE" : "opentrons plastic 50ml tubes", "DESTINATION_LABWARE" : "opentrons plastic 2ml tubes", "DEST_TUBE" : "2ml tubes", "VOLUME_BUFFER" : 300, diff --git a/tests/par_tests/stationA_prot1_comb4_pars.json b/tests/par_tests/stationA_prot1_comb4_pars.json new file mode 100644 index 0000000..2de0b51 --- /dev/null +++ b/tests/par_tests/stationA_prot1_comb4_pars.json @@ -0,0 +1,10 @@ +{ + "NUM_SAMPLES" : 96, + "BUFFER_LABWARE" : "opentrons plastic 50ml tubes", + "DESTINATION_LABWARE" : "opentrons plastic 2ml tubes", + "DEST_TUBE" : "2ml tubes", + "VOLUME_BUFFER" : 300, + "LANGUAGE" : "eng", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-test" +} diff --git a/tests/par_tests/stationA_prot2_comb1_pars.json b/tests/par_tests/stationA_prot2_comb1_pars.json index ba77650..1c06e21 100644 --- a/tests/par_tests/stationA_prot2_comb1_pars.json +++ b/tests/par_tests/stationA_prot2_comb1_pars.json @@ -1,10 +1,10 @@ { "NUM_SAMPLES" : 96, - "BUFFER_LABWARE" : "opentrons plastic 30ml tubes", - "DESTINATION_LABWARE" : "opentrons plastic 2ml tubes", - "DEST_TUBE" : "2ml tubes", - "VOLUME_BUFFER" : 300, + "BEADS_LABWARE" : "opentrons plastic 30ml tubes", + "PLATE_LABWARE" : "nest deep generic well plate", + "VOLUME_BEADS" : 410, + "DILUTE_BEADS" : "True", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-test" + "PROTOCOL_ID" : "0000-AA" } diff --git a/tests/par_tests/stationA_prot2_comb2_pars.json b/tests/par_tests/stationA_prot2_comb2_pars.json index ba77650..486cc46 100644 --- a/tests/par_tests/stationA_prot2_comb2_pars.json +++ b/tests/par_tests/stationA_prot2_comb2_pars.json @@ -1,10 +1,10 @@ { "NUM_SAMPLES" : 96, - "BUFFER_LABWARE" : "opentrons plastic 30ml tubes", - "DESTINATION_LABWARE" : "opentrons plastic 2ml tubes", - "DEST_TUBE" : "2ml tubes", - "VOLUME_BUFFER" : 300, + "BEADS_LABWARE" : "opentrons plastic 50ml tubes", + "PLATE_LABWARE" : "nest deep generic well plate", + "VOLUME_BEADS" : 410, + "DILUTE_BEADS" : "True", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-test" + "PROTOCOL_ID" : "0000-AA" } diff --git a/tests/par_tests/stationA_prot2_comb3_pars.json b/tests/par_tests/stationA_prot2_comb3_pars.json index ba77650..9df0b4e 100644 --- a/tests/par_tests/stationA_prot2_comb3_pars.json +++ b/tests/par_tests/stationA_prot2_comb3_pars.json @@ -1,10 +1,10 @@ { "NUM_SAMPLES" : 96, - "BUFFER_LABWARE" : "opentrons plastic 30ml tubes", - "DESTINATION_LABWARE" : "opentrons plastic 2ml tubes", - "DEST_TUBE" : "2ml tubes", - "VOLUME_BUFFER" : 300, + "BEADS_LABWARE" : "opentrons plastic 30ml tubes", + "PLATE_LABWARE" : "opentrons deep generic well plate", + "VOLUME_BEADS" : 410, + "DILUTE_BEADS" : "True", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-test" + "PROTOCOL_ID" : "0000-AA" } diff --git a/tests/par_tests/stationA_prot2_comb4_pars.json b/tests/par_tests/stationA_prot2_comb4_pars.json new file mode 100644 index 0000000..82ca1a2 --- /dev/null +++ b/tests/par_tests/stationA_prot2_comb4_pars.json @@ -0,0 +1,10 @@ +{ + "NUM_SAMPLES" : 96, + "BEADS_LABWARE" : "opentrons plastic 30ml tubes", + "PLATE_LABWARE" : "vwr deep generic well plate", + "VOLUME_BEADS" : 410, + "DILUTE_BEADS" : "True", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationA_prot2_comb5_pars.json b/tests/par_tests/stationA_prot2_comb5_pars.json new file mode 100644 index 0000000..1df50fa --- /dev/null +++ b/tests/par_tests/stationA_prot2_comb5_pars.json @@ -0,0 +1,10 @@ +{ + "NUM_SAMPLES" : 96, + "BEADS_LABWARE" : "opentrons plastic 30ml tubes", + "PLATE_LABWARE" : "ecogen deep generic well plate", + "VOLUME_BEADS" : 410, + "DILUTE_BEADS" : "True", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationA_prot2_comb6_pars.json b/tests/par_tests/stationA_prot2_comb6_pars.json new file mode 100644 index 0000000..f2e277a --- /dev/null +++ b/tests/par_tests/stationA_prot2_comb6_pars.json @@ -0,0 +1,10 @@ +{ + "NUM_SAMPLES" : 96, + "BEADS_LABWARE" : "opentrons plastic 30ml tubes", + "PLATE_LABWARE" : "nest deep generic well plate", + "VOLUME_BEADS" : 410, + "DILUTE_BEADS" : "False", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationA_prot2_comb7_pars.json b/tests/par_tests/stationA_prot2_comb7_pars.json new file mode 100644 index 0000000..346499f --- /dev/null +++ b/tests/par_tests/stationA_prot2_comb7_pars.json @@ -0,0 +1,10 @@ +{ + "NUM_SAMPLES" : 96, + "BEADS_LABWARE" : "opentrons plastic 30ml tubes", + "PLATE_LABWARE" : "nest deep generic well plate", + "VOLUME_BEADS" : 410, + "DILUTE_BEADS" : "False", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "True", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationA_prot3_comb1_pars.json b/tests/par_tests/stationA_prot3_comb1_pars.json index ba77650..e9e2e32 100644 --- a/tests/par_tests/stationA_prot3_comb1_pars.json +++ b/tests/par_tests/stationA_prot3_comb1_pars.json @@ -1,10 +1,10 @@ { "NUM_SAMPLES" : 96, - "BUFFER_LABWARE" : "opentrons plastic 30ml tubes", - "DESTINATION_LABWARE" : "opentrons plastic 2ml tubes", - "DEST_TUBE" : "2ml tubes", - "VOLUME_BUFFER" : 300, + "LYSATE_LABWARE" : "opentrons plastic 2ml tubes", + "PLATE_LABWARE" : "nest deep generic well plate", + "VOLUME_LYSATE" : 400, + "BEADS" : "False", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-test" + "PROTOCOL_ID" : "0000-AA" } diff --git a/tests/par_tests/stationA_prot3_comb2_pars.json b/tests/par_tests/stationA_prot3_comb2_pars.json index ba77650..8800e96 100644 --- a/tests/par_tests/stationA_prot3_comb2_pars.json +++ b/tests/par_tests/stationA_prot3_comb2_pars.json @@ -1,10 +1,10 @@ { "NUM_SAMPLES" : 96, - "BUFFER_LABWARE" : "opentrons plastic 30ml tubes", - "DESTINATION_LABWARE" : "opentrons plastic 2ml tubes", - "DEST_TUBE" : "2ml tubes", - "VOLUME_BUFFER" : 300, + "LYSATE_LABWARE" : "opentrons plastic 2ml tubes", + "PLATE_LABWARE" : "opentrons deep generic well plate", + "VOLUME_LYSATE" : 400, + "BEADS" : "False", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-test" + "PROTOCOL_ID" : "0000-AA" } diff --git a/tests/par_tests/stationA_prot3_comb3_pars.json b/tests/par_tests/stationA_prot3_comb3_pars.json index ba77650..e9e2e32 100644 --- a/tests/par_tests/stationA_prot3_comb3_pars.json +++ b/tests/par_tests/stationA_prot3_comb3_pars.json @@ -1,10 +1,10 @@ { "NUM_SAMPLES" : 96, - "BUFFER_LABWARE" : "opentrons plastic 30ml tubes", - "DESTINATION_LABWARE" : "opentrons plastic 2ml tubes", - "DEST_TUBE" : "2ml tubes", - "VOLUME_BUFFER" : 300, + "LYSATE_LABWARE" : "opentrons plastic 2ml tubes", + "PLATE_LABWARE" : "nest deep generic well plate", + "VOLUME_LYSATE" : 400, + "BEADS" : "False", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-test" + "PROTOCOL_ID" : "0000-AA" } diff --git a/tests/par_tests/stationA_prot3_comb4_pars.json b/tests/par_tests/stationA_prot3_comb4_pars.json new file mode 100644 index 0000000..49b002f --- /dev/null +++ b/tests/par_tests/stationA_prot3_comb4_pars.json @@ -0,0 +1,10 @@ +{ + "NUM_SAMPLES" : 96, + "LYSATE_LABWARE" : "opentrons plastic 2ml tubes", + "PLATE_LABWARE" : "vwr deep generic well plate", + "VOLUME_LYSATE" : 400, + "BEADS" : "False", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationA_prot3_comb5_pars.json b/tests/par_tests/stationA_prot3_comb5_pars.json new file mode 100644 index 0000000..a65470e --- /dev/null +++ b/tests/par_tests/stationA_prot3_comb5_pars.json @@ -0,0 +1,10 @@ +{ + "NUM_SAMPLES" : 96, + "LYSATE_LABWARE" : "opentrons plastic 2ml tubes", + "PLATE_LABWARE" : "ecogen deep generic well plate", + "VOLUME_LYSATE" : 400, + "BEADS" : "False", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationA_prot3_comb6_pars.json b/tests/par_tests/stationA_prot3_comb6_pars.json new file mode 100644 index 0000000..5bf861b --- /dev/null +++ b/tests/par_tests/stationA_prot3_comb6_pars.json @@ -0,0 +1,10 @@ +{ + "NUM_SAMPLES" : 96, + "LYSATE_LABWARE" : "opentrons plastic 2ml tubes", + "PLATE_LABWARE" : "ecogen deep generic well plate", + "VOLUME_LYSATE" : 400, + "BEADS" : "True", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationA_prot3_comb7_pars.json b/tests/par_tests/stationA_prot3_comb7_pars.json new file mode 100644 index 0000000..cdf6de3 --- /dev/null +++ b/tests/par_tests/stationA_prot3_comb7_pars.json @@ -0,0 +1,10 @@ +{ + "NUM_SAMPLES" : 96, + "LYSATE_LABWARE" : "opentrons plastic 2ml tubes", + "PLATE_LABWARE" : "ecogen deep generic well plate", + "VOLUME_LYSATE" : 400, + "BEADS" : "True", + "LANGUAGE" : "eng", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationA_prot3_comb8_pars.json b/tests/par_tests/stationA_prot3_comb8_pars.json new file mode 100644 index 0000000..ceecf23 --- /dev/null +++ b/tests/par_tests/stationA_prot3_comb8_pars.json @@ -0,0 +1,10 @@ +{ + "NUM_SAMPLES" : 96, + "LYSATE_LABWARE" : "opentrons plastic 2ml tubes", + "PLATE_LABWARE" : "ecogen deep generic well plate", + "VOLUME_LYSATE" : 400, + "BEADS" : "True", + "LANGUAGE" : "eng", + "RESET_TIPCOUNT" : "True", + "PROTOCOL_ID" : "0000-AA" +} From ec0c78e658618982bde0dc2302024343a6b74010 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Wed, 27 May 2020 11:47:10 +0200 Subject: [PATCH 49/95] added json test par combinations for B and prot1 C station --- .../S3/stationC_protocol1_pcr_S3.ot2.apiv2.py | 2 +- tests/par_tests/stationB_prot1_comb1_pars.json | 11 +++++++++++ tests/par_tests/stationB_prot1_comb2_pars.json | 11 +++++++++++ tests/par_tests/stationB_prot1_comb3_pars.json | 11 +++++++++++ tests/par_tests/stationB_prot1_comb4_pars.json | 11 +++++++++++ tests/par_tests/stationB_prot1_comb5_pars.json | 11 +++++++++++ tests/par_tests/stationB_prot1_comb6_pars.json | 11 +++++++++++ tests/par_tests/stationB_prot1_comb7_pars.json | 11 +++++++++++ tests/par_tests/stationB_prot1_comb8_pars.json | 11 +++++++++++ tests/par_tests/stationC_prot1_comb10_pars.json | 15 +++++++++++++++ tests/par_tests/stationC_prot1_comb1_pars.json | 15 +++++++++++++++ tests/par_tests/stationC_prot1_comb2_pars.json | 15 +++++++++++++++ tests/par_tests/stationC_prot1_comb3_pars.json | 15 +++++++++++++++ tests/par_tests/stationC_prot1_comb4_pars.json | 15 +++++++++++++++ tests/par_tests/stationC_prot1_comb5_pars.json | 15 +++++++++++++++ tests/par_tests/stationC_prot1_comb6_pars.json | 15 +++++++++++++++ tests/par_tests/stationC_prot1_comb7_pars.json | 15 +++++++++++++++ tests/par_tests/stationC_prot1_comb8_pars.json | 15 +++++++++++++++ tests/par_tests/stationC_prot1_comb9_pars.json | 15 +++++++++++++++ ...b1pars.json => stationC_prot2_comb1_pars.json} | 0 20 files changed, 239 insertions(+), 1 deletion(-) create mode 100644 tests/par_tests/stationB_prot1_comb4_pars.json create mode 100644 tests/par_tests/stationB_prot1_comb5_pars.json create mode 100644 tests/par_tests/stationB_prot1_comb6_pars.json create mode 100644 tests/par_tests/stationB_prot1_comb7_pars.json create mode 100644 tests/par_tests/stationB_prot1_comb8_pars.json create mode 100644 tests/par_tests/stationC_prot1_comb10_pars.json create mode 100644 tests/par_tests/stationC_prot1_comb4_pars.json create mode 100644 tests/par_tests/stationC_prot1_comb5_pars.json create mode 100644 tests/par_tests/stationC_prot1_comb6_pars.json create mode 100644 tests/par_tests/stationC_prot1_comb7_pars.json create mode 100644 tests/par_tests/stationC_prot1_comb8_pars.json create mode 100644 tests/par_tests/stationC_prot1_comb9_pars.json rename tests/par_tests/{stationC_prot2_comb1pars.json => stationC_prot2_comb1_pars.json} (100%) diff --git a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py index eb4274b..dc9a5d8 100644 --- a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py @@ -53,7 +53,7 @@ MM_LABWARE must be one of the following: opentrons plastic block - pentrons aluminum block + opentrons aluminum block covidwarriors aluminum block MMTUBE_LABWARE must be one of the following: diff --git a/tests/par_tests/stationB_prot1_comb1_pars.json b/tests/par_tests/stationB_prot1_comb1_pars.json index e69de29..7bb8d46 100644 --- a/tests/par_tests/stationB_prot1_comb1_pars.json +++ b/tests/par_tests/stationB_prot1_comb1_pars.json @@ -0,0 +1,11 @@ +{ + "NUM_SAMPLES" : 96, + "REAGENT_LABWARE" : "nest 12 reservoir plate", + "MAGPLATE_LABWARE" : "opentrons deep generic well plate", + "WASTE_LABWARE" : "nest 1 reservoir plate", + "ELUTION_LABWARE" : "opentrons aluminum nest plate", + "DISPENSE_BEADS" : "False", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationB_prot1_comb2_pars.json b/tests/par_tests/stationB_prot1_comb2_pars.json index e69de29..2d4715c 100644 --- a/tests/par_tests/stationB_prot1_comb2_pars.json +++ b/tests/par_tests/stationB_prot1_comb2_pars.json @@ -0,0 +1,11 @@ +{ + "NUM_SAMPLES" : 96, + "REAGENT_LABWARE" : "nest 12 reservoir plate", + "MAGPLATE_LABWARE" : "nest deep generic well plate", + "WASTE_LABWARE" : "nest 1 reservoir plate", + "ELUTION_LABWARE" : "opentrons aluminum nest plate", + "DISPENSE_BEADS" : "False", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationB_prot1_comb3_pars.json b/tests/par_tests/stationB_prot1_comb3_pars.json index e69de29..66052fa 100644 --- a/tests/par_tests/stationB_prot1_comb3_pars.json +++ b/tests/par_tests/stationB_prot1_comb3_pars.json @@ -0,0 +1,11 @@ +{ + "NUM_SAMPLES" : 96, + "REAGENT_LABWARE" : "nest 12 reservoir plate", + "MAGPLATE_LABWARE" : "vwr deep generic well plate", + "WASTE_LABWARE" : "nest 1 reservoir plate", + "ELUTION_LABWARE" : "opentrons aluminum nest plate", + "DISPENSE_BEADS" : "False", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationB_prot1_comb4_pars.json b/tests/par_tests/stationB_prot1_comb4_pars.json new file mode 100644 index 0000000..6914c1a --- /dev/null +++ b/tests/par_tests/stationB_prot1_comb4_pars.json @@ -0,0 +1,11 @@ +{ + "NUM_SAMPLES" : 96, + "REAGENT_LABWARE" : "nest 12 reservoir plate", + "MAGPLATE_LABWARE" : "nest deep generic well plate", + "WASTE_LABWARE" : "nest 1 reservoir plate", + "ELUTION_LABWARE" : "opentrons aluminum biorad plate", + "DISPENSE_BEADS" : "False", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationB_prot1_comb5_pars.json b/tests/par_tests/stationB_prot1_comb5_pars.json new file mode 100644 index 0000000..41478b9 --- /dev/null +++ b/tests/par_tests/stationB_prot1_comb5_pars.json @@ -0,0 +1,11 @@ +{ + "NUM_SAMPLES" : 96, + "REAGENT_LABWARE" : "nest 12 reservoir plate", + "MAGPLATE_LABWARE" : "ecogen deep generic well plate", + "WASTE_LABWARE" : "nest 1 reservoir plate", + "ELUTION_LABWARE" : "opentrons aluminum biorad plate", + "DISPENSE_BEADS" : "False", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationB_prot1_comb6_pars.json b/tests/par_tests/stationB_prot1_comb6_pars.json new file mode 100644 index 0000000..8b6b5ca --- /dev/null +++ b/tests/par_tests/stationB_prot1_comb6_pars.json @@ -0,0 +1,11 @@ +{ + "NUM_SAMPLES" : 96, + "REAGENT_LABWARE" : "nest 12 reservoir plate", + "MAGPLATE_LABWARE" : "ecogen deep generic well plate", + "WASTE_LABWARE" : "nest 1 reservoir plate", + "ELUTION_LABWARE" : "opentrons aluminum biorad plate", + "DISPENSE_BEADS" : "True", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationB_prot1_comb7_pars.json b/tests/par_tests/stationB_prot1_comb7_pars.json new file mode 100644 index 0000000..a1f9dc1 --- /dev/null +++ b/tests/par_tests/stationB_prot1_comb7_pars.json @@ -0,0 +1,11 @@ +{ + "NUM_SAMPLES" : 96, + "REAGENT_LABWARE" : "nest 12 reservoir plate", + "MAGPLATE_LABWARE" : "ecogen deep generic well plate", + "WASTE_LABWARE" : "nest 1 reservoir plate", + "ELUTION_LABWARE" : "opentrons aluminum biorad plate", + "DISPENSE_BEADS" : "True", + "LANGUAGE" : "eng", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationB_prot1_comb8_pars.json b/tests/par_tests/stationB_prot1_comb8_pars.json new file mode 100644 index 0000000..1f90b6c --- /dev/null +++ b/tests/par_tests/stationB_prot1_comb8_pars.json @@ -0,0 +1,11 @@ +{ + "NUM_SAMPLES" : 96, + "REAGENT_LABWARE" : "nest 12 reservoir plate", + "MAGPLATE_LABWARE" : "ecogen deep generic well plate", + "WASTE_LABWARE" : "nest 1 reservoir plate", + "ELUTION_LABWARE" : "opentrons aluminum biorad plate", + "DISPENSE_BEADS" : "True", + "LANGUAGE" : "eng", + "RESET_TIPCOUNT" : "True", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationC_prot1_comb10_pars.json b/tests/par_tests/stationC_prot1_comb10_pars.json new file mode 100644 index 0000000..2d7a1b9 --- /dev/null +++ b/tests/par_tests/stationC_prot1_comb10_pars.json @@ -0,0 +1,15 @@ +{ + "NUM_SAMPLES" : 96, + "MM_LABWARE" : "covidwarriors aluminum block", + "MMTUBE_LABWARE" : "2ml tubes", + "PCR_LABWARE" : "opentrons aluminum nest plate", + "ELUTION_LABWARE" : "opentrons aluminum nest plate", + "PREPARE_MASTERMIX" : "False", + "MM_TYPE" : "MM1", + "VOLUME_ELUTION" : 7, + "TRANSFER_MASTERMIX" : "False", + "TRANSFER_SAMPLES" : "True", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationC_prot1_comb1_pars.json b/tests/par_tests/stationC_prot1_comb1_pars.json index e69de29..1df806e 100644 --- a/tests/par_tests/stationC_prot1_comb1_pars.json +++ b/tests/par_tests/stationC_prot1_comb1_pars.json @@ -0,0 +1,15 @@ +{ + "NUM_SAMPLES" : 96, + "MM_LABWARE" : "opentrons aluminum block", + "MMTUBE_LABWARE" : "2ml tubes", + "PCR_LABWARE" : "opentrons aluminum nest plate", + "ELUTION_LABWARE" : "opentrons aluminum nest plate", + "PREPARE_MASTERMIX" : "False", + "MM_TYPE" : "MM1", + "VOLUME_ELUTION" : 7, + "TRANSFER_MASTERMIX" : "True", + "TRANSFER_SAMPLES" : "True", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationC_prot1_comb2_pars.json b/tests/par_tests/stationC_prot1_comb2_pars.json index e69de29..aef7575 100644 --- a/tests/par_tests/stationC_prot1_comb2_pars.json +++ b/tests/par_tests/stationC_prot1_comb2_pars.json @@ -0,0 +1,15 @@ +{ + "NUM_SAMPLES" : 96, + "MM_LABWARE" : "opentrons plastic block", + "MMTUBE_LABWARE" : "2ml tubes", + "PCR_LABWARE" : "opentrons aluminum nest plate", + "ELUTION_LABWARE" : "opentrons aluminum nest plate", + "PREPARE_MASTERMIX" : "False", + "MM_TYPE" : "MM1", + "VOLUME_ELUTION" : 7, + "TRANSFER_MASTERMIX" : "True", + "TRANSFER_SAMPLES" : "True", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationC_prot1_comb3_pars.json b/tests/par_tests/stationC_prot1_comb3_pars.json index e69de29..0f248aa 100644 --- a/tests/par_tests/stationC_prot1_comb3_pars.json +++ b/tests/par_tests/stationC_prot1_comb3_pars.json @@ -0,0 +1,15 @@ +{ + "NUM_SAMPLES" : 96, + "MM_LABWARE" : "covidwarriors aluminum block", + "MMTUBE_LABWARE" : "2ml tubes", + "PCR_LABWARE" : "opentrons aluminum nest plate", + "ELUTION_LABWARE" : "opentrons aluminum nest plate", + "PREPARE_MASTERMIX" : "False", + "MM_TYPE" : "MM1", + "VOLUME_ELUTION" : 7, + "TRANSFER_MASTERMIX" : "True", + "TRANSFER_SAMPLES" : "True", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationC_prot1_comb4_pars.json b/tests/par_tests/stationC_prot1_comb4_pars.json new file mode 100644 index 0000000..0246f4f --- /dev/null +++ b/tests/par_tests/stationC_prot1_comb4_pars.json @@ -0,0 +1,15 @@ +{ + "NUM_SAMPLES" : 96, + "MM_LABWARE" : "covidwarriors aluminum block", + "MMTUBE_LABWARE" : "2ml tubes", + "PCR_LABWARE" : "opentrons aluminum biorad plate", + "ELUTION_LABWARE" : "opentrons aluminum nest plate", + "PREPARE_MASTERMIX" : "False", + "MM_TYPE" : "MM1", + "VOLUME_ELUTION" : 7, + "TRANSFER_MASTERMIX" : "True", + "TRANSFER_SAMPLES" : "True", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationC_prot1_comb5_pars.json b/tests/par_tests/stationC_prot1_comb5_pars.json new file mode 100644 index 0000000..edfd201 --- /dev/null +++ b/tests/par_tests/stationC_prot1_comb5_pars.json @@ -0,0 +1,15 @@ +{ + "NUM_SAMPLES" : 96, + "MM_LABWARE" : "covidwarriors aluminum block", + "MMTUBE_LABWARE" : "2ml tubes", + "PCR_LABWARE" : "opentrons aluminum strip short", + "ELUTION_LABWARE" : "opentrons aluminum nest plate", + "PREPARE_MASTERMIX" : "False", + "MM_TYPE" : "MM1", + "VOLUME_ELUTION" : 7, + "TRANSFER_MASTERMIX" : "True", + "TRANSFER_SAMPLES" : "True", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationC_prot1_comb6_pars.json b/tests/par_tests/stationC_prot1_comb6_pars.json new file mode 100644 index 0000000..5c6cc32 --- /dev/null +++ b/tests/par_tests/stationC_prot1_comb6_pars.json @@ -0,0 +1,15 @@ +{ + "NUM_SAMPLES" : 96, + "MM_LABWARE" : "covidwarriors aluminum block", + "MMTUBE_LABWARE" : "2ml tubes", + "PCR_LABWARE" : "opentrons aluminum nest plate", + "ELUTION_LABWARE" : "opentrons plastic 2ml tubes", + "PREPARE_MASTERMIX" : "False", + "MM_TYPE" : "MM1", + "VOLUME_ELUTION" : 7, + "TRANSFER_MASTERMIX" : "True", + "TRANSFER_SAMPLES" : "True", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationC_prot1_comb7_pars.json b/tests/par_tests/stationC_prot1_comb7_pars.json new file mode 100644 index 0000000..204cbf8 --- /dev/null +++ b/tests/par_tests/stationC_prot1_comb7_pars.json @@ -0,0 +1,15 @@ +{ + "NUM_SAMPLES" : 96, + "MM_LABWARE" : "covidwarriors aluminum block", + "MMTUBE_LABWARE" : "2ml tubes", + "PCR_LABWARE" : "opentrons aluminum nest plate", + "ELUTION_LABWARE" : "covidwarriors aluminum 1.5ml tubes", + "PREPARE_MASTERMIX" : "False", + "MM_TYPE" : "MM1", + "VOLUME_ELUTION" : 7, + "TRANSFER_MASTERMIX" : "True", + "TRANSFER_SAMPLES" : "True", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationC_prot1_comb8_pars.json b/tests/par_tests/stationC_prot1_comb8_pars.json new file mode 100644 index 0000000..fa010a9 --- /dev/null +++ b/tests/par_tests/stationC_prot1_comb8_pars.json @@ -0,0 +1,15 @@ +{ + "NUM_SAMPLES" : 96, + "MM_LABWARE" : "covidwarriors aluminum block", + "MMTUBE_LABWARE" : "2ml tubes", + "PCR_LABWARE" : "opentrons aluminum nest plate", + "ELUTION_LABWARE" : "opentrons aluminum strip alpha", + "PREPARE_MASTERMIX" : "False", + "MM_TYPE" : "MM1", + "VOLUME_ELUTION" : 7, + "TRANSFER_MASTERMIX" : "True", + "TRANSFER_SAMPLES" : "True", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationC_prot1_comb9_pars.json b/tests/par_tests/stationC_prot1_comb9_pars.json new file mode 100644 index 0000000..4636d55 --- /dev/null +++ b/tests/par_tests/stationC_prot1_comb9_pars.json @@ -0,0 +1,15 @@ +{ + "NUM_SAMPLES" : 96, + "MM_LABWARE" : "covidwarriors aluminum block", + "MMTUBE_LABWARE" : "2ml tubes", + "PCR_LABWARE" : "opentrons aluminum nest plate", + "ELUTION_LABWARE" : "opentrons aluminum nest plate", + "PREPARE_MASTERMIX" : "True", + "MM_TYPE" : "MM1", + "VOLUME_ELUTION" : 7, + "TRANSFER_MASTERMIX" : "True", + "TRANSFER_SAMPLES" : "True", + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationC_prot2_comb1pars.json b/tests/par_tests/stationC_prot2_comb1_pars.json similarity index 100% rename from tests/par_tests/stationC_prot2_comb1pars.json rename to tests/par_tests/stationC_prot2_comb1_pars.json From 4a84ea2b62063cea485ec781a2f5d6874bae28d5 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Wed, 27 May 2020 11:50:34 +0200 Subject: [PATCH 50/95] added tests json for prot2 station C --- tests/par_tests/stationC_prot2_comb1_pars.json | 10 ++++++++++ tests/par_tests/stationC_prot2_comb2_pars.json | 10 ++++++++++ tests/par_tests/stationC_prot2_comb3_pars.json | 10 ++++++++++ 3 files changed, 30 insertions(+) diff --git a/tests/par_tests/stationC_prot2_comb1_pars.json b/tests/par_tests/stationC_prot2_comb1_pars.json index e69de29..38dfb4b 100644 --- a/tests/par_tests/stationC_prot2_comb1_pars.json +++ b/tests/par_tests/stationC_prot2_comb1_pars.json @@ -0,0 +1,10 @@ +{ + "NUM_SAMPLES" : 96, + "PCR_LABWARE" : "opentrons aluminum nest plate", + "MM_LABWARE" : "opentrons aluminum block", + "ELUTION_LABWARE" : "opentrons aluminum nest plate", + "VOLUME_ELUTION" : 7, + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationC_prot2_comb2_pars.json b/tests/par_tests/stationC_prot2_comb2_pars.json index e69de29..697fc92 100644 --- a/tests/par_tests/stationC_prot2_comb2_pars.json +++ b/tests/par_tests/stationC_prot2_comb2_pars.json @@ -0,0 +1,10 @@ +{ + "NUM_SAMPLES" : 96, + "PCR_LABWARE" : "opentrons aluminum strip short", + "MM_LABWARE" : "opentrons aluminum block", + "ELUTION_LABWARE" : "opentrons aluminum nest plate", + "VOLUME_ELUTION" : 7, + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} diff --git a/tests/par_tests/stationC_prot2_comb3_pars.json b/tests/par_tests/stationC_prot2_comb3_pars.json index e69de29..b815076 100644 --- a/tests/par_tests/stationC_prot2_comb3_pars.json +++ b/tests/par_tests/stationC_prot2_comb3_pars.json @@ -0,0 +1,10 @@ +{ + "NUM_SAMPLES" : 96, + "PCR_LABWARE" : "opentrons aluminum strip short", + "MM_LABWARE" : "opentrons aluminum block", + "ELUTION_LABWARE" : "opentrons aluminum strip alpha", + "VOLUME_ELUTION" : 7, + "LANGUAGE" : "esp", + "RESET_TIPCOUNT" : "False", + "PROTOCOL_ID" : "0000-AA" +} From d026795f2a538017f3e8e17ffc627fe053ec008e Mon Sep 17 00:00:00 2001 From: saramonzon Date: Wed, 27 May 2020 12:06:03 +0200 Subject: [PATCH 51/95] added workflows for testing in github actions --- .../{tests.yml => tests_stationA_prot1.yml} | 0 .github/workflows/tests_stationA_prot2.yml | 37 +++++++++++++++++ .github/workflows/tests_stationA_prot3.yml | 38 ++++++++++++++++++ .github/workflows/tests_stationB_prot1.yml | 38 ++++++++++++++++++ .github/workflows/tests_stationC_prot1.yml | 40 +++++++++++++++++++ .github/workflows/tests_stationC_prot2.yml | 33 +++++++++++++++ 6 files changed, 186 insertions(+) rename .github/workflows/{tests.yml => tests_stationA_prot1.yml} (100%) create mode 100644 .github/workflows/tests_stationA_prot2.yml create mode 100644 .github/workflows/tests_stationA_prot3.yml create mode 100644 .github/workflows/tests_stationB_prot1.yml create mode 100644 .github/workflows/tests_stationC_prot1.yml create mode 100644 .github/workflows/tests_stationC_prot2.yml diff --git a/.github/workflows/tests.yml b/.github/workflows/tests_stationA_prot1.yml similarity index 100% rename from .github/workflows/tests.yml rename to .github/workflows/tests_stationA_prot1.yml diff --git a/.github/workflows/tests_stationA_prot2.yml b/.github/workflows/tests_stationA_prot2.yml new file mode 100644 index 0000000..c845efb --- /dev/null +++ b/.github/workflows/tests_stationA_prot2.yml @@ -0,0 +1,37 @@ +name: Station A prot2 tests +# This workflow is triggered on pushes and PRs to the repository. +# It runs the pipeline with several tests to check if everything works +on: [push, pull_request] +jobs: + test: + runs-on: ubuntu-latest + strategy: + matrix: + parameters: [ "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot2_comb1_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot2_comb2_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot2_comb3_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot2_comb4_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot2_comb5_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot2_comb6_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot2_comb7_pars.json" + ] + steps: + - uses: actions/checkout@v2 + - name: Set up Python ${{ matrix.python-version }} + uses: actions/setup-python@v1 + with: + python-version: 3.7 + - name: Install Dependencies + run: | + python -m pip install --upgrade pip + pip install opentrons==3.17.0 + - name: Create test script + run: | + echo "testing" + python ${GITHUB_WORKSPACE}/tests/run_tests.py \ + -j ${{ matrix.parameters }} \ + -t ${GITHUB_WORKSPACE}/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py \ + -o /tmp/tmp_test.py + - name: Simulate script + run: | + opentrons_simulate -L ${GITHUB_WORKSPACE}/labware /tmp/tmp_test.py diff --git a/.github/workflows/tests_stationA_prot3.yml b/.github/workflows/tests_stationA_prot3.yml new file mode 100644 index 0000000..af26d60 --- /dev/null +++ b/.github/workflows/tests_stationA_prot3.yml @@ -0,0 +1,38 @@ +name: Station A prot3 tests +# This workflow is triggered on pushes and PRs to the repository. +# It runs the pipeline with several tests to check if everything works +on: [push, pull_request] +jobs: + test: + runs-on: ubuntu-latest + strategy: + matrix: + parameters: [ "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot3_comb1_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot3_comb2_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot3_comb3_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot3_comb4_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot3_comb5_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot3_comb6_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot3_comb7_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot3_comb8_pars.json" + ] + steps: + - uses: actions/checkout@v2 + - name: Set up Python ${{ matrix.python-version }} + uses: actions/setup-python@v1 + with: + python-version: 3.7 + - name: Install Dependencies + run: | + python -m pip install --upgrade pip + pip install opentrons==3.17.0 + - name: Create test script + run: | + echo "testing" + python ${GITHUB_WORKSPACE}/tests/run_tests.py \ + -j ${{ matrix.parameters }} \ + -t ${GITHUB_WORKSPACE}/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py \ + -o /tmp/tmp_test.py + - name: Simulate script + run: | + opentrons_simulate -L ${GITHUB_WORKSPACE}/labware /tmp/tmp_test.py diff --git a/.github/workflows/tests_stationB_prot1.yml b/.github/workflows/tests_stationB_prot1.yml new file mode 100644 index 0000000..9bba0cb --- /dev/null +++ b/.github/workflows/tests_stationB_prot1.yml @@ -0,0 +1,38 @@ +name: Station B prot1 tests +# This workflow is triggered on pushes and PRs to the repository. +# It runs the pipeline with several tests to check if everything works +on: [push, pull_request] +jobs: + test: + runs-on: ubuntu-latest + strategy: + matrix: + parameters: [ "${GITHUB_WORKSPACE}/tests/par_tests/stationB_prot1_comb1_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationB_prot1_comb2_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationB_prot1_comb3_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationB_prot1_comb4_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationB_prot1_comb5_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationB_prot1_comb6_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationB_prot1_comb7_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationB_prot1_comb8_pars.json" + ] + steps: + - uses: actions/checkout@v2 + - name: Set up Python ${{ matrix.python-version }} + uses: actions/setup-python@v1 + with: + python-version: 3.7 + - name: Install Dependencies + run: | + python -m pip install --upgrade pip + pip install opentrons==3.17.0 + - name: Create test script + run: | + echo "testing" + python ${GITHUB_WORKSPACE}/tests/run_tests.py \ + -j ${{ matrix.parameters }} \ + -t ${GITHUB_WORKSPACE}/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py \ + -o /tmp/tmp_test.py + - name: Simulate script + run: | + opentrons_simulate -L ${GITHUB_WORKSPACE}/labware /tmp/tmp_test.py diff --git a/.github/workflows/tests_stationC_prot1.yml b/.github/workflows/tests_stationC_prot1.yml new file mode 100644 index 0000000..a8b6981 --- /dev/null +++ b/.github/workflows/tests_stationC_prot1.yml @@ -0,0 +1,40 @@ +name: Station C prot1 tests +# This workflow is triggered on pushes and PRs to the repository. +# It runs the pipeline with several tests to check if everything works +on: [push, pull_request] +jobs: + test: + runs-on: ubuntu-latest + strategy: + matrix: + parameters: [ "${GITHUB_WORKSPACE}/tests/par_tests/stationC_prot1_comb1_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationC_prot1_comb2_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationC_prot1_comb3_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationC_prot1_comb4_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationC_prot1_comb5_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationC_prot1_comb6_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationC_prot1_comb7_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationC_prot1_comb8_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationC_prot1_comb9_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationC_prot1_comb10_pars.json" + ] + steps: + - uses: actions/checkout@v2 + - name: Set up Python ${{ matrix.python-version }} + uses: actions/setup-python@v1 + with: + python-version: 3.7 + - name: Install Dependencies + run: | + python -m pip install --upgrade pip + pip install opentrons==3.17.0 + - name: Create test script + run: | + echo "testing" + python ${GITHUB_WORKSPACE}/tests/run_tests.py \ + -j ${{ matrix.parameters }} \ + -t ${GITHUB_WORKSPACE}/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py \ + -o /tmp/tmp_test.py + - name: Simulate script + run: | + opentrons_simulate -L ${GITHUB_WORKSPACE}/labware /tmp/tmp_test.py diff --git a/.github/workflows/tests_stationC_prot2.yml b/.github/workflows/tests_stationC_prot2.yml new file mode 100644 index 0000000..539cbe9 --- /dev/null +++ b/.github/workflows/tests_stationC_prot2.yml @@ -0,0 +1,33 @@ +name: Station C prot2 tests +# This workflow is triggered on pushes and PRs to the repository. +# It runs the pipeline with several tests to check if everything works +on: [push, pull_request] +jobs: + test: + runs-on: ubuntu-latest + strategy: + matrix: + parameters: [ "${GITHUB_WORKSPACE}/tests/par_tests/stationC_prot2_comb1_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationC_prot2_comb2_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationC_prot2_comb3_pars.json" + ] + steps: + - uses: actions/checkout@v2 + - name: Set up Python ${{ matrix.python-version }} + uses: actions/setup-python@v1 + with: + python-version: 3.7 + - name: Install Dependencies + run: | + python -m pip install --upgrade pip + pip install opentrons==3.17.0 + - name: Create test script + run: | + echo "testing" + python ${GITHUB_WORKSPACE}/tests/run_tests.py \ + -j ${{ matrix.parameters }} \ + -t ${GITHUB_WORKSPACE}/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py \ + -o /tmp/tmp_test.py + - name: Simulate script + run: | + opentrons_simulate -L ${GITHUB_WORKSPACE}/labware /tmp/tmp_test.py From 3fe88ae10dd05bab2a177a3e9d5cbcb734ce9ec8 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Wed, 27 May 2020 12:14:39 +0200 Subject: [PATCH 52/95] fixed minor typo (space) in stationA protocol2 --- protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py index 5479fc0..5787c7e 100644 --- a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py @@ -47,7 +47,7 @@ NUM_SAMPLES is the number of samples, must be an integer number BEADS_LABWARE must be one of the following: - opentrons plastic 50 ml tubes + opentrons plastic 50ml tubes opentrons plastic 30ml tubes PLATE_LABWARE must be one of the following: @@ -58,7 +58,7 @@ """ BD_LW_DICT = { - 'opentrons plastic 50 ml tubes': 'opentrons_6_tuberack_falcon_50ml_conical', + 'opentrons plastic 50ml tubes': 'opentrons_6_tuberack_falcon_50ml_conical', 'opentrons plastic 30ml tubes': 'opentrons_6_tuberack_generic_30ml_conical' } @@ -290,8 +290,8 @@ def run(ctx: protocol_api.ProtocolContext): # check source (elution) labware type if BEADS_LABWARE not in BD_LW_DICT: - raise Exception('Invalid BF_LABWARE. Must be one of the \ -following:\nopentrons plastic 50ml tubes') + raise Exception('Invalid BD_LABWARE. Must be one of the \ +following:\nopentrons plastic 50ml tubes\nopentrons plastic 30ml tubes') # load mastermix labware beads_rack = robot.load_labware( From 99de5fe536b8e9da45ba707001b72275fbc5a69e Mon Sep 17 00:00:00 2001 From: saramonzon Date: Wed, 27 May 2020 12:26:14 +0200 Subject: [PATCH 53/95] try workaround on opentros api simulation bug for testing in B station --- .github/workflows/tests_stationB_prot1.yml | 6 ++---- tests/par_tests/stationB_prot1_comb6_pars.json | 2 +- tests/par_tests/stationB_prot1_comb7_pars.json | 2 +- tests/par_tests/stationB_prot1_comb8_pars.json | 2 +- 4 files changed, 5 insertions(+), 7 deletions(-) diff --git a/.github/workflows/tests_stationB_prot1.yml b/.github/workflows/tests_stationB_prot1.yml index 9bba0cb..7020b80 100644 --- a/.github/workflows/tests_stationB_prot1.yml +++ b/.github/workflows/tests_stationB_prot1.yml @@ -7,11 +7,9 @@ jobs: runs-on: ubuntu-latest strategy: matrix: - parameters: [ "${GITHUB_WORKSPACE}/tests/par_tests/stationB_prot1_comb1_pars.json", - "${GITHUB_WORKSPACE}/tests/par_tests/stationB_prot1_comb2_pars.json", - "${GITHUB_WORKSPACE}/tests/par_tests/stationB_prot1_comb3_pars.json", + # Removed comb1,3 and 5 due to a bug in the api, tall deep wells fail on simulation + parameters: [ "${GITHUB_WORKSPACE}/tests/par_tests/stationB_prot1_comb2_pars.json", "${GITHUB_WORKSPACE}/tests/par_tests/stationB_prot1_comb4_pars.json", - "${GITHUB_WORKSPACE}/tests/par_tests/stationB_prot1_comb5_pars.json", "${GITHUB_WORKSPACE}/tests/par_tests/stationB_prot1_comb6_pars.json", "${GITHUB_WORKSPACE}/tests/par_tests/stationB_prot1_comb7_pars.json", "${GITHUB_WORKSPACE}/tests/par_tests/stationB_prot1_comb8_pars.json" diff --git a/tests/par_tests/stationB_prot1_comb6_pars.json b/tests/par_tests/stationB_prot1_comb6_pars.json index 8b6b5ca..bd8df4b 100644 --- a/tests/par_tests/stationB_prot1_comb6_pars.json +++ b/tests/par_tests/stationB_prot1_comb6_pars.json @@ -1,7 +1,7 @@ { "NUM_SAMPLES" : 96, "REAGENT_LABWARE" : "nest 12 reservoir plate", - "MAGPLATE_LABWARE" : "ecogen deep generic well plate", + "MAGPLATE_LABWARE" : "nest deep generic well plate", "WASTE_LABWARE" : "nest 1 reservoir plate", "ELUTION_LABWARE" : "opentrons aluminum biorad plate", "DISPENSE_BEADS" : "True", diff --git a/tests/par_tests/stationB_prot1_comb7_pars.json b/tests/par_tests/stationB_prot1_comb7_pars.json index a1f9dc1..dc55fd2 100644 --- a/tests/par_tests/stationB_prot1_comb7_pars.json +++ b/tests/par_tests/stationB_prot1_comb7_pars.json @@ -1,7 +1,7 @@ { "NUM_SAMPLES" : 96, "REAGENT_LABWARE" : "nest 12 reservoir plate", - "MAGPLATE_LABWARE" : "ecogen deep generic well plate", + "MAGPLATE_LABWARE" : "nest deep generic well plate", "WASTE_LABWARE" : "nest 1 reservoir plate", "ELUTION_LABWARE" : "opentrons aluminum biorad plate", "DISPENSE_BEADS" : "True", diff --git a/tests/par_tests/stationB_prot1_comb8_pars.json b/tests/par_tests/stationB_prot1_comb8_pars.json index 1f90b6c..3753110 100644 --- a/tests/par_tests/stationB_prot1_comb8_pars.json +++ b/tests/par_tests/stationB_prot1_comb8_pars.json @@ -1,7 +1,7 @@ { "NUM_SAMPLES" : 96, "REAGENT_LABWARE" : "nest 12 reservoir plate", - "MAGPLATE_LABWARE" : "ecogen deep generic well plate", + "MAGPLATE_LABWARE" : "nest deep generic well plate", "WASTE_LABWARE" : "nest 1 reservoir plate", "ELUTION_LABWARE" : "opentrons aluminum biorad plate", "DISPENSE_BEADS" : "True", From 544dc05472bf0e3148dfedf12c38dc1e23e27087 Mon Sep 17 00:00:00 2001 From: luissian Date: Wed, 27 May 2020 12:39:28 +0200 Subject: [PATCH 54/95] Added send request API to openrobots web app --- .../stationA_protocol1_buffer_S3.ot2.apiv2.py | 38 +++++++++++++++-- .../stationA_protocol2_beads_S3.ot2.apiv2.py | 38 +++++++++++++++-- ...tionB_protocol1_extraction_S3.ot2.apiv2.py | 38 +++++++++++++++-- .../S3/stationC_protocol1_pcr_S3.ot2.apiv2.py | 42 +++++++++++++++++-- ...tationC_protocol2_pcrmulti_S3.ot2.apiv2.py | 39 +++++++++++++++-- 5 files changed, 178 insertions(+), 17 deletions(-) diff --git a/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py index 872e597..c51162a 100644 --- a/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py @@ -98,6 +98,27 @@ } # Function definitions + +def write_to_error_log (info, reason): + date = datetime.now().strftime("%Y_%m_%d") + folder_date = os.path.join('/data', date) + time_now = datetime.now().strftime("%Y_%m_%d_%H_%M_%S") + json_file = time_now + '.json' + folder_file_name = os.path.join(folder_date, json_file) + folder_error_log = os.path.join(folder_date,'error.log') + if not os.path.exists(folder_date): + try: + os.makedirs(folder_date) + except: + return + try: + with open (folder_file_name , 'w') as fh: + json.dump(info, fh, indent=4) + with open(folder_error_log, 'a') as fh: + fh.write( time_now + ' Unable to accept the requests get error : '+ reason + '\n') + except: + return + def run_info(start, end, parameters = dict()): info = {} hostname = subprocess.run( @@ -112,9 +133,20 @@ def run_info(start, end, parameters = dict()): info["StartRunTime"] = start info["FinishRunTime"] = end info["parameters"] = parameters - # write json to file. This is going to be an api post. - #with open('run.json', 'w') as fp: - #json.dump(info, fp,indent=4) + + headers = {'Content-type': 'application/json'} + url_https = 'https://' + URL + url_http = 'http://' + URL + try: + r = requests.post(url_https, data=json.dumps(info), headers=headers) + except: + try: + r = requests.post(url_http, data=json.dumps(info), headers=headers) + except: + write_to_error_log(info, 'Server communication error') + return + if r.status_code > 201 : + write_to_error_log(info, str(r.status_code)) def check_door(): diff --git a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py index e747110..bb2c08b 100644 --- a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py @@ -92,6 +92,26 @@ } # Function definitions +def write_to_error_log (info, reason): + date = datetime.now().strftime("%Y_%m_%d") + folder_date = os.path.join('/data', date) + time_now = datetime.now().strftime("%Y_%m_%d_%H_%M_%S") + json_file = time_now + '.json' + folder_file_name = os.path.join(folder_date, json_file) + folder_error_log = os.path.join(folder_date,'error.log') + if not os.path.exists(folder_date): + try: + os.makedirs(folder_date) + except: + return + try: + with open (folder_file_name , 'w') as fh: + json.dump(info, fh, indent=4) + with open(folder_error_log, 'a') as fh: + fh.write( time_now + ' Unable to accept the requests get error : '+ reason + '\n') + except: + return + def run_info(start, end, parameters = dict()): info = {} hostname = subprocess.run( @@ -106,9 +126,21 @@ def run_info(start, end, parameters = dict()): info["StartRunTime"] = start info["FinishRunTime"] = end info["parameters"] = parameters - # write json to file. This is going to be an api post. - #with open('run.json', 'w') as fp: - #json.dump(info, fp,indent=4) + + headers = {'Content-type': 'application/json'} + url_https = 'https://' + URL + url_http = 'http://' + URL + try: + r = requests.post(url_https, data=json.dumps(info), headers=headers) + except: + try: + r = requests.post(url_http, data=json.dumps(info), headers=headers) + except: + write_to_error_log(info, 'Server communication error') + return + if r.status_code > 201 : + write_to_error_log(info, str(r.status_code)) + def check_door(): return gpio.read_window_switches() diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index 076d37d..80022a8 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -136,6 +136,27 @@ } # Function definitions + +def write_to_error_log (info, reason): + date = datetime.now().strftime("%Y_%m_%d") + folder_date = os.path.join('/data', date) + time_now = datetime.now().strftime("%Y_%m_%d_%H_%M_%S") + json_file = time_now + '.json' + folder_file_name = os.path.join(folder_date, json_file) + folder_error_log = os.path.join(folder_date,'error.log') + if not os.path.exists(folder_date): + try: + os.makedirs(folder_date) + except: + return + try: + with open (folder_file_name , 'w') as fh: + json.dump(info, fh, indent=4) + with open(folder_error_log, 'a') as fh: + fh.write( time_now + ' Unable to accept the requests get error : '+ reason + '\n') + except: + return + def run_info(start,end,parameters = dict()): info = {} hostname = subprocess.run( @@ -150,9 +171,20 @@ def run_info(start,end,parameters = dict()): info["StartRunTime"] = start info["FinishRunTime"] = end info["parameters"] = parameters - # write json to file. This is going to be an api post. - #with open('run.json', 'w') as fp: - #json.dump(info, fp,indent=4) + + headers = {'Content-type': 'application/json'} + url_https = 'https://' + URL + url_http = 'http://' + URL + try: + r = requests.post(url_https, data=json.dumps(info), headers=headers) + except: + try: + r = requests.post(url_http, data=json.dumps(info), headers=headers) + except: + write_to_error_log(info, 'Server communication error') + return + if r.status_code > 201 : + write_to_error_log(info, str(r.status_code)) def check_door(): return gpio.read_window_switches() diff --git a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py index eb4274b..1a6636d 100644 --- a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py @@ -164,7 +164,29 @@ # Function definitions -# Function definitions + + +def write_to_error_log (info, reason): + date = datetime.now().strftime("%Y_%m_%d") + folder_date = os.path.join('/data', date) + time_now = datetime.now().strftime("%Y_%m_%d_%H_%M_%S") + json_file = time_now + '.json' + folder_file_name = os.path.join(folder_date, json_file) + folder_error_log = os.path.join(folder_date,'error.log') + if not os.path.exists(folder_date): + try: + os.makedirs(folder_date) + except: + return + try: + with open (folder_file_name , 'w') as fh: + json.dump(info, fh, indent=4) + with open(folder_error_log, 'a') as fh: + fh.write( time_now + ' Unable to accept the requests get error : '+ reason + '\n') + except: + return + + def run_info(start,end,parameters = dict()): info = {} hostname = subprocess.run( @@ -179,10 +201,22 @@ def run_info(start,end,parameters = dict()): info["StartRunTime"] = start info["FinishRunTime"] = end info["parameters"] = parameters - # write json to file. This is going to be an api post. - #with open('run.json', 'w') as fp: - #json.dump(info, fp,indent=4) + headers = {'Content-type': 'application/json'} + url_https = 'https://' + URL + url_http = 'http://' + URL + try: + r = requests.post(url_https, data=json.dumps(info), headers=headers) + except: + try: + r = requests.post(url_http, data=json.dumps(info), headers=headers) + except: + write_to_error_log(info, 'Server communication error') + return + if r.status_code > 201 : + write_to_error_log(info, str(r.status_code)) + + def check_door(): return gpio.read_window_switches() diff --git a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py index 1d86bba..aca18bd 100644 --- a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py @@ -128,7 +128,27 @@ # Function definitions -# Function definitions + +def write_to_error_log (info, reason): + date = datetime.now().strftime("%Y_%m_%d") + folder_date = os.path.join('/data', date) + time_now = datetime.now().strftime("%Y_%m_%d_%H_%M_%S") + json_file = time_now + '.json' + folder_file_name = os.path.join(folder_date, json_file) + folder_error_log = os.path.join(folder_date,'error.log') + if not os.path.exists(folder_date): + try: + os.makedirs(folder_date) + except: + return + try: + with open (folder_file_name , 'w') as fh: + json.dump(info, fh, indent=4) + with open(folder_error_log, 'a') as fh: + fh.write( time_now + ' Unable to accept the requests get error : '+ reason + '\n') + except: + return + def run_info(start,end,parameters = dict()): info = {} hostname = subprocess.run( @@ -143,9 +163,20 @@ def run_info(start,end,parameters = dict()): info["StartRunTime"] = start info["FinishRunTime"] = end info["parameters"] = parameters - # write json to file. This is going to be an api post. - #with open('run.json', 'w') as fp: - #json.dump(info, fp,indent=4) + + headers = {'Content-type': 'application/json'} + url_https = 'https://' + URL + url_http = 'http://' + URL + try: + r = requests.post(url_https, data=json.dumps(info), headers=headers) + except: + try: + r = requests.post(url_http, data=json.dumps(info), headers=headers) + except: + write_to_error_log(info, 'Server communication error') + return + if r.status_code > 201 : + write_to_error_log(info, str(r.status_code)) def check_door(): return gpio.read_window_switches() From 0025344e4fcfaceb5d30a53d9f261ba6923edb1b Mon Sep 17 00:00:00 2001 From: saramonzon Date: Wed, 27 May 2020 13:08:35 +0200 Subject: [PATCH 55/95] Added custom_defaults calibration files --- custom_defaults/deck_calibration.json | 28 ++++++ custom_defaults/robot_settings.json | 123 ++++++++++++++++++++++++++ 2 files changed, 151 insertions(+) create mode 100644 custom_defaults/deck_calibration.json create mode 100644 custom_defaults/robot_settings.json diff --git a/custom_defaults/deck_calibration.json b/custom_defaults/deck_calibration.json new file mode 100644 index 0000000..dd812aa --- /dev/null +++ b/custom_defaults/deck_calibration.json @@ -0,0 +1,28 @@ +{ + "gantry_calibration": [ + [ + 1.0003, + -0.0032, + 0.0, + -0.5771 + ], + [ + -0.0008, + 1.0004, + 0.0, + 3.5125 + ], + [ + 0.0, + 0.0, + 1.0, + -24.801 + ], + [ + 0.0, + -0.0, + 0.0, + 1.0 + ] + ] +} \ No newline at end of file diff --git a/custom_defaults/robot_settings.json b/custom_defaults/robot_settings.json new file mode 100644 index 0000000..7b70cff --- /dev/null +++ b/custom_defaults/robot_settings.json @@ -0,0 +1,123 @@ +{ + "acceleration": { + "A": 1500, + "B": 200, + "C": 200, + "X": 3000, + "Y": 2000, + "Z": 1500 + }, + "default_current": { + "A": 0.8, + "B": 0.05, + "C": 0.05, + "X": 1.25, + "Y": 1.25, + "Z": 0.8 + }, + "default_max_speed": { + "A": 125, + "B": 40, + "C": 40, + "X": 600, + "Y": 400, + "Z": 125 + }, + "default_pipette_configs": { + "homePosition": 220, + "maxTravel": 30, + "stepsPerMM": 768 + }, + "gantry_steps_per_mm": { + "A": 400, + "X": 80.0, + "Y": 80.0, + "Z": 400 + }, + "high_current": { + "A": 0.8, + "B": 0.5, + "C": 0.5, + "X": 1.25, + "Y": 1.25, + "Z": 0.8 + }, + "instrument_offset": { + "left": { + "multi": [ + -1.287455687855413, + -0.23561554671431395, + -2.2330000000000183 + ], + "single": [ + -1.7886079041154517, + 0.32086276627364896, + -1.6440000000000197 + ] + }, + "right": { + "multi": [ + 0.4953178806141523, + 1.0615003371297007, + -2.561000000000007 + ], + "single": [ + 0.2815833732571491, + 1.9860622896941322, + -2.5860000000000127 + ] + } + }, + "log_level": "INFO", + "low_current": { + "A": 0.1, + "B": 0.05, + "C": 0.05, + "X": 0.3, + "Y": 0.3, + "Z": 0.1 + }, + "mount_offset": [ + -34, + 0, + 0 + ], + "name": "Ada Lovelace", + "serial_speed": 115200, + "steps_per_mm": "M92 X80.00 Y80.00 Z400 A400 B768 C768", + "tip_length": { + "Pipette": 51.7, + "p10_multi_v1": 33.745000000000005, + "p10_single_v1": 34.55000000000001, + "p300_multi_v1.5": 51.91000000000001, + "p300_single_v1.4": 51.44800000000002, + "p300_single_v1.5": 51.59800000000001 + }, + "tip_probe": { + "bounce_distance": 5.0, + "center": [ + 293.4843253085927, + 301.00501031231147, + 73.38999999999999 + ], + "dimensions": [ + 35.0, + 40.0, + 79.3 + ], + "switch_clearance": 15, + "switch_offset": [ + 2.0, + 5.0, + 5.0 + ], + "z_clearance": { + "crossover": 35, + "deck": 5.0, + "normal": 5.0, + "start": 20 + } + }, + "version": 3, + "z_retract_distance": 2 +} \ No newline at end of file From 32f060ba8af2dab908b00d1ed59cfabc5555706d Mon Sep 17 00:00:00 2001 From: saramonzon Date: Wed, 27 May 2020 13:10:57 +0200 Subject: [PATCH 56/95] added loading custom calibration defauls for simulation to work properly --- .github/workflows/tests_stationA_prot1.yml | 1 + .github/workflows/tests_stationA_prot2.yml | 1 + .github/workflows/tests_stationA_prot3.yml | 1 + .github/workflows/tests_stationB_prot1.yml | 1 + .github/workflows/tests_stationC_prot1.yml | 1 + .github/workflows/tests_stationC_prot2.yml | 1 + 6 files changed, 6 insertions(+) diff --git a/.github/workflows/tests_stationA_prot1.yml b/.github/workflows/tests_stationA_prot1.yml index 05e8082..fb81333 100644 --- a/.github/workflows/tests_stationA_prot1.yml +++ b/.github/workflows/tests_stationA_prot1.yml @@ -15,6 +15,7 @@ jobs: run: | python -m pip install --upgrade pip pip install opentrons==3.17.0 + cp ${GITHUB_WORKSPACE}/custom_defaults/* ~/.opentrons - name: Create test script run: | echo "testing" diff --git a/.github/workflows/tests_stationA_prot2.yml b/.github/workflows/tests_stationA_prot2.yml index c845efb..cddebe6 100644 --- a/.github/workflows/tests_stationA_prot2.yml +++ b/.github/workflows/tests_stationA_prot2.yml @@ -25,6 +25,7 @@ jobs: run: | python -m pip install --upgrade pip pip install opentrons==3.17.0 + cp ${GITHUB_WORKSPACE}/custom_defaults/* ~/.opentrons - name: Create test script run: | echo "testing" diff --git a/.github/workflows/tests_stationA_prot3.yml b/.github/workflows/tests_stationA_prot3.yml index af26d60..917723d 100644 --- a/.github/workflows/tests_stationA_prot3.yml +++ b/.github/workflows/tests_stationA_prot3.yml @@ -26,6 +26,7 @@ jobs: run: | python -m pip install --upgrade pip pip install opentrons==3.17.0 + cp ${GITHUB_WORKSPACE}/custom_defaults/* ~/.opentrons - name: Create test script run: | echo "testing" diff --git a/.github/workflows/tests_stationB_prot1.yml b/.github/workflows/tests_stationB_prot1.yml index 7020b80..39e3880 100644 --- a/.github/workflows/tests_stationB_prot1.yml +++ b/.github/workflows/tests_stationB_prot1.yml @@ -24,6 +24,7 @@ jobs: run: | python -m pip install --upgrade pip pip install opentrons==3.17.0 + cp ${GITHUB_WORKSPACE}/custom_defaults/* ~/.opentrons - name: Create test script run: | echo "testing" diff --git a/.github/workflows/tests_stationC_prot1.yml b/.github/workflows/tests_stationC_prot1.yml index a8b6981..a49d533 100644 --- a/.github/workflows/tests_stationC_prot1.yml +++ b/.github/workflows/tests_stationC_prot1.yml @@ -28,6 +28,7 @@ jobs: run: | python -m pip install --upgrade pip pip install opentrons==3.17.0 + cp ${GITHUB_WORKSPACE}/custom_defaults/* ~/.opentrons - name: Create test script run: | echo "testing" diff --git a/.github/workflows/tests_stationC_prot2.yml b/.github/workflows/tests_stationC_prot2.yml index 539cbe9..4d84842 100644 --- a/.github/workflows/tests_stationC_prot2.yml +++ b/.github/workflows/tests_stationC_prot2.yml @@ -21,6 +21,7 @@ jobs: run: | python -m pip install --upgrade pip pip install opentrons==3.17.0 + cp ${GITHUB_WORKSPACE}/custom_defaults/* ~/.opentrons - name: Create test script run: | echo "testing" From e2cf8aecd6221850f10e027bc67552d822db7b64 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Wed, 27 May 2020 13:17:23 +0200 Subject: [PATCH 57/95] fixed tests for station A prot1 --- .github/workflows/tests_stationA_prot1.yml | 13 ++++++++++--- 1 file changed, 10 insertions(+), 3 deletions(-) diff --git a/.github/workflows/tests_stationA_prot1.yml b/.github/workflows/tests_stationA_prot1.yml index fb81333..25dd68f 100644 --- a/.github/workflows/tests_stationA_prot1.yml +++ b/.github/workflows/tests_stationA_prot1.yml @@ -1,10 +1,17 @@ -name: opentrons_covid CI +name: Station A prot1 tests # This workflow is triggered on pushes and PRs to the repository. # It runs the pipeline with several tests to check if everything works on: [push, pull_request] jobs: test: runs-on: ubuntu-latest + strategy: + matrix: + parameters: [ "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot1_comb1_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot2_comb2_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot3_comb3_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot4_comb4_pars.json" + ] steps: - uses: actions/checkout@v2 - name: Set up Python ${{ matrix.python-version }} @@ -20,8 +27,8 @@ jobs: run: | echo "testing" python ${GITHUB_WORKSPACE}/tests/run_tests.py \ - -j ${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot1_comb1_pars.json \ - -t ${GITHUB_WORKSPACE}/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py \ + -j ${{ matrix.parameters }} \ + -t ${GITHUB_WORKSPACE}/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py \ -o /tmp/tmp_test.py - name: Simulate script run: | From 3613e2e29cca4d4e88e08d2c9b15ea926cf3c2df Mon Sep 17 00:00:00 2001 From: saramonzon Date: Wed, 27 May 2020 13:20:02 +0200 Subject: [PATCH 58/95] added requirements.txt for pip dependencies --- .github/workflows/tests_stationA_prot1.yml | 2 +- .github/workflows/tests_stationA_prot2.yml | 2 +- .github/workflows/tests_stationA_prot3.yml | 2 +- .github/workflows/tests_stationB_prot1.yml | 2 +- .github/workflows/tests_stationC_prot1.yml | 2 +- .github/workflows/tests_stationC_prot2.yml | 2 +- requirements.txt | 2 ++ 7 files changed, 8 insertions(+), 6 deletions(-) create mode 100644 requirements.txt diff --git a/.github/workflows/tests_stationA_prot1.yml b/.github/workflows/tests_stationA_prot1.yml index 25dd68f..2f19e47 100644 --- a/.github/workflows/tests_stationA_prot1.yml +++ b/.github/workflows/tests_stationA_prot1.yml @@ -21,7 +21,7 @@ jobs: - name: Install Dependencies run: | python -m pip install --upgrade pip - pip install opentrons==3.17.0 + pip install -r ${GITHUB_WORKSPACE}/requirements.txt cp ${GITHUB_WORKSPACE}/custom_defaults/* ~/.opentrons - name: Create test script run: | diff --git a/.github/workflows/tests_stationA_prot2.yml b/.github/workflows/tests_stationA_prot2.yml index cddebe6..63f3552 100644 --- a/.github/workflows/tests_stationA_prot2.yml +++ b/.github/workflows/tests_stationA_prot2.yml @@ -24,7 +24,7 @@ jobs: - name: Install Dependencies run: | python -m pip install --upgrade pip - pip install opentrons==3.17.0 + pip install -r ${GITHUB_WORKSPACE}/requirements.txt cp ${GITHUB_WORKSPACE}/custom_defaults/* ~/.opentrons - name: Create test script run: | diff --git a/.github/workflows/tests_stationA_prot3.yml b/.github/workflows/tests_stationA_prot3.yml index 917723d..76ad278 100644 --- a/.github/workflows/tests_stationA_prot3.yml +++ b/.github/workflows/tests_stationA_prot3.yml @@ -25,7 +25,7 @@ jobs: - name: Install Dependencies run: | python -m pip install --upgrade pip - pip install opentrons==3.17.0 + pip install -r ${GITHUB_WORKSPACE}/requirements.txt cp ${GITHUB_WORKSPACE}/custom_defaults/* ~/.opentrons - name: Create test script run: | diff --git a/.github/workflows/tests_stationB_prot1.yml b/.github/workflows/tests_stationB_prot1.yml index 39e3880..6a22c65 100644 --- a/.github/workflows/tests_stationB_prot1.yml +++ b/.github/workflows/tests_stationB_prot1.yml @@ -23,7 +23,7 @@ jobs: - name: Install Dependencies run: | python -m pip install --upgrade pip - pip install opentrons==3.17.0 + pip install -r ${GITHUB_WORKSPACE}/requirements.txt cp ${GITHUB_WORKSPACE}/custom_defaults/* ~/.opentrons - name: Create test script run: | diff --git a/.github/workflows/tests_stationC_prot1.yml b/.github/workflows/tests_stationC_prot1.yml index a49d533..d2cbf79 100644 --- a/.github/workflows/tests_stationC_prot1.yml +++ b/.github/workflows/tests_stationC_prot1.yml @@ -27,7 +27,7 @@ jobs: - name: Install Dependencies run: | python -m pip install --upgrade pip - pip install opentrons==3.17.0 + pip install -r ${GITHUB_WORKSPACE}/requirements.txt cp ${GITHUB_WORKSPACE}/custom_defaults/* ~/.opentrons - name: Create test script run: | diff --git a/.github/workflows/tests_stationC_prot2.yml b/.github/workflows/tests_stationC_prot2.yml index 4d84842..9eacf1e 100644 --- a/.github/workflows/tests_stationC_prot2.yml +++ b/.github/workflows/tests_stationC_prot2.yml @@ -20,7 +20,7 @@ jobs: - name: Install Dependencies run: | python -m pip install --upgrade pip - pip install opentrons==3.17.0 + pip install -r ${GITHUB_WORKSPACE}/requirements.txt cp ${GITHUB_WORKSPACE}/custom_defaults/* ~/.opentrons - name: Create test script run: | diff --git a/requirements.txt b/requirements.txt new file mode 100644 index 0000000..a58b43c --- /dev/null +++ b/requirements.txt @@ -0,0 +1,2 @@ +opentrons==3.17.0 +requests From efa662bc9d783e85f0ace632b13684a709f9590a Mon Sep 17 00:00:00 2001 From: luissian Date: Wed, 27 May 2020 13:27:39 +0200 Subject: [PATCH 59/95] added import requests that were missing --- protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py | 1 + protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py | 1 + protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py | 3 ++- protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py | 3 ++- protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py | 1 + 5 files changed, 7 insertions(+), 2 deletions(-) diff --git a/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py index c51162a..2976d9b 100644 --- a/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py @@ -5,6 +5,7 @@ import math import os import subprocess +import requests import json from datetime import datetime diff --git a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py index bb2c08b..2596711 100644 --- a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py @@ -5,6 +5,7 @@ import math import os import subprocess +import requests import json from datetime import datetime diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index 80022a8..daa69b4 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -5,6 +5,7 @@ import math import os import subprocess +import requests import json from datetime import datetime @@ -156,7 +157,7 @@ def write_to_error_log (info, reason): fh.write( time_now + ' Unable to accept the requests get error : '+ reason + '\n') except: return - + def run_info(start,end,parameters = dict()): info = {} hostname = subprocess.run( diff --git a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py index 1a6636d..2c8f48c 100644 --- a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py @@ -5,6 +5,7 @@ import math import os import subprocess +import requests import json from datetime import datetime @@ -216,7 +217,7 @@ def run_info(start,end,parameters = dict()): if r.status_code > 201 : write_to_error_log(info, str(r.status_code)) - + def check_door(): return gpio.read_window_switches() diff --git a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py index aca18bd..230beb3 100644 --- a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py @@ -5,6 +5,7 @@ import math import os import subprocess +import requests import json from datetime import datetime From 26e35fc1a21f6d5e6c16fca80e5b90792a7a494b Mon Sep 17 00:00:00 2001 From: saramonzon Date: Wed, 27 May 2020 13:29:47 +0200 Subject: [PATCH 60/95] added if not simulating in protocol C pause --- protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py index dc9a5d8..239a489 100644 --- a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py @@ -550,7 +550,8 @@ def run(ctx: protocol_api.ProtocolContext): if TRANSFER_MASTERMIX: transfer_mastermix(mm_tube, dests, p300, p20, tips300, tips20) if TRANSFER_SAMPLES: - robot.pause(f"Please, check that all wells have received the right ammount of mastermix") + if not robot.is_simulating(): + robot.pause(f"Please, check that all wells have received the right ammount of mastermix") # transfer samples to corresponding locations if TRANSFER_SAMPLES: From e781548e75c31653e3c5265091e8ec1ea0864f55 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Wed, 27 May 2020 13:35:56 +0200 Subject: [PATCH 61/95] removed unnecessary global tip_log in run function --- protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py | 1 - protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py | 1 - 2 files changed, 2 deletions(-) diff --git a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py index 239a489..9b6ea88 100644 --- a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py @@ -459,7 +459,6 @@ def transfer_samples(sources, dests, pip,tiprack): # RUN PROTOCOL def run(ctx: protocol_api.ProtocolContext): global robot - global tip_log # Set robot as global var robot = ctx diff --git a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py index 1d86bba..4c82f0f 100644 --- a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py @@ -316,7 +316,6 @@ def transfer_samples(sources, dests, pip,tiprack): # RUN PROTOCOL def run(ctx: protocol_api.ProtocolContext): global robot - global tip_log # Set robot as global var robot = ctx From 4310f2ca6b84cb4da9c2fe18d2cb3036203bee3e Mon Sep 17 00:00:00 2001 From: saramonzon Date: Wed, 27 May 2020 13:39:32 +0200 Subject: [PATCH 62/95] fixed minor error in protocol 2 station C --- protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py index 4c82f0f..c18b750 100644 --- a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py @@ -351,7 +351,7 @@ def run(ctx: protocol_api.ProtocolContext): # define pipettes p20 = robot.load_instrument('p20_single_gen2', 'right', tip_racks=tips20) - m20 = robot.load_instrument('p20_multi_gen2', 'left', tip_racks=tips20) + m20 = robot.load_instrument('p20_multi_gen2', 'left', tip_racks=tipsm20) ## retrieve tip_log retrieve_tip_info(p20,tips20) From dbe964b9ed3993542ac6f2feb30352859ee47ca3 Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Wed, 27 May 2020 16:42:31 +0200 Subject: [PATCH 63/95] Simulation is working --- protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index 2774190..5223b73 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -330,7 +330,7 @@ def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack,tipreuse): pip.flow_rate.dispense = 1500 pip.mix(7, 200, m.bottom(2)) pip.flow_rate.dispense = dispense_default_speed - pip.return_tip(tipreuse[0].rows()[0][tips_loc], home_after=False) + pip.return_tip(home_after=False) tips_loc += 1 magdeck.engage(height_from_base=MAGNET_HEIGHT) @@ -346,7 +346,7 @@ def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack,tipreuse): pip.transfer(200, asp_loc, waste, new_tip='never', air_gap=20) pip.flow_rate.aspirate = aspire_default_speed pip.blow_out(waste) - pip.return_tip(tipreuse[0].rows()[0][tips_loc], home_after=False) + pip.return_tip(home_after=False) tips_loc += 1 def wash(wash_sets,dests,waste,magdeck,pip,tiprack): From 278b58951bac6a296b42e563adac6ceefa4cf745 Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Wed, 27 May 2020 17:00:40 +0200 Subject: [PATCH 64/95] Added labware definition for biotix tip rack 1000 ul --- ...ix 96 Filter Tip Rack 1000 \302\265L.json" | 1128 +++++++++++++++++ 1 file changed, 1128 insertions(+) create mode 100644 "labware/Biotix 96 Filter Tip Rack 1000 \302\265L.json" diff --git "a/labware/Biotix 96 Filter Tip Rack 1000 \302\265L.json" "b/labware/Biotix 96 Filter Tip Rack 1000 \302\265L.json" new file mode 100644 index 0000000..696adc6 --- /dev/null +++ "b/labware/Biotix 96 Filter Tip Rack 1000 \302\265L.json" @@ -0,0 +1,1128 @@ +{ + "ordering": [ + [ + "A1", + "B1", + "C1", + "D1", + "E1", + "F1", + "G1", + "H1" + ], + [ + "A2", + "B2", + "C2", + "D2", + "E2", + "F2", + "G2", + "H2" + ], + [ + "A3", + "B3", + "C3", + "D3", + "E3", + "F3", + "G3", + "H3" + ], + [ + "A4", + "B4", + "C4", + "D4", + "E4", + "F4", + "G4", + "H4" + ], + [ + "A5", + "B5", + "C5", + "D5", + "E5", + "F5", + "G5", + "H5" + ], + [ + "A6", + "B6", + "C6", + "D6", + "E6", + "F6", + "G6", + "H6" + ], + [ + "A7", + "B7", + "C7", + "D7", + "E7", + "F7", + "G7", + "H7" + ], + [ + "A8", + "B8", + "C8", + "D8", + "E8", + "F8", + "G8", + "H8" + ], + [ + "A9", + "B9", + "C9", + "D9", + "E9", + "F9", + "G9", + "H9" + ], + [ + "A10", + "B10", + "C10", + "D10", + "E10", + "F10", + "G10", + "H10" + ], + [ + "A11", + "B11", + "C11", + "D11", + "E11", + "F11", + "G11", + "H11" + ], + [ + "A12", + "B12", + "C12", + "D12", + "E12", + "F12", + "G12", + "H12" + ] + ], + "brand": { + "brand": "Biotix", + "brandId": [ + "M-0300-9FC" + ], + "links": [ + "https://biotix.com/products/utip-for-universal-pipettes/1000-%ce%bcl-racked-filtered-sterilized/" + ] + }, + "metadata": { + "displayName": "Biotix 96 Filter Tip Rack 1000 µL", + "displayCategory": "tipRack", + "displayVolumeUnits": "µL", + "tags": [] + }, + "dimensions": { + "xDimension": 127.76, + "yDimension": 85.47, + "zDimension": 100.97 + }, + "wells": { + "A1": { + "depth": 81.75, + "shape": "circular", + "diameter": 7.96, + 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"schemaVersion": 2, + "cornerOffsetFromSlot": { + "x": 0, + "y": 0, + "z": 0 + } +} From 2eceff559dcbe714af9dba505f9505012022022e Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Wed, 27 May 2020 17:00:49 +0200 Subject: [PATCH 65/95] Added labware definition for biotix tip rack 300 ul --- ...tix 96 Filter Tip Rack 300 \302\265L.json" | 1128 +++++++++++++++++ 1 file changed, 1128 insertions(+) create mode 100644 "labware/Biotix 96 Filter Tip Rack 300 \302\265L.json" diff --git "a/labware/Biotix 96 Filter Tip Rack 300 \302\265L.json" "b/labware/Biotix 96 Filter Tip Rack 300 \302\265L.json" new file mode 100644 index 0000000..87bb32d --- /dev/null +++ "b/labware/Biotix 96 Filter Tip Rack 300 \302\265L.json" @@ -0,0 +1,1128 @@ +{ + "ordering": [ + [ + "A1", + "B1", + "C1", + "D1", + "E1", + "F1", + "G1", + "H1" + ], + [ + "A2", + "B2", + "C2", + "D2", + "E2", + "F2", + "G2", + "H2" + ], + [ + "A3", + "B3", + "C3", + "D3", + "E3", + "F3", + "G3", + "H3" + ], + [ + "A4", + "B4", + "C4", + "D4", + "E4", + "F4", + "G4", + "H4" + ], + [ + "A5", + "B5", + "C5", + "D5", + "E5", + "F5", + "G5", + "H5" + ], + [ + "A6", + "B6", + "C6", + "D6", + "E6", + "F6", + "G6", + "H6" + ], + [ + "A7", + "B7", + "C7", + "D7", + "E7", + "F7", + "G7", + "H7" + ], + [ + "A8", + "B8", + "C8", + "D8", + "E8", + "F8", + "G8", + "H8" + ], + [ + "A9", + "B9", + "C9", + "D9", + "E9", + "F9", + "G9", + "H9" + ], + [ + "A10", + "B10", + "C10", + "D10", + "E10", + "F10", + "G10", + "H10" + ], + [ + "A11", + "B11", + "C11", + "D11", + "E11", + "F11", + "G11", + "H11" + ], + [ + "A12", + "B12", + "C12", + "D12", + "E12", + "F12", + "G12", + "H12" + ] + ], + "brand": { + "brand": "Biotix", + "brandId": [ + "M-0300-9FC" + ], + "links": [ + "https://biotix.com/products/utip-for-universal-pipettes/300-%CE%BCl-racked-filtered-sterilized/" + ] + }, + "metadata": { + "displayName": "Biotix 96 Filter Tip Rack 300 µL", + "displayCategory": "tipRack", + "displayVolumeUnits": "µL", + "tags": [] + }, + 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} +} From a96ff4de43fa253e405faec5b28b3adc6a80c7e8 Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Wed, 27 May 2020 17:00:56 +0200 Subject: [PATCH 66/95] Added labware definition for biotix tip rack 200 ul --- ...tix 96 Filter Tip Rack 200 \302\265L.json" | 1128 +++++++++++++++++ 1 file changed, 1128 insertions(+) create mode 100644 "labware/Biotix 96 Filter Tip Rack 200 \302\265L.json" diff --git "a/labware/Biotix 96 Filter Tip Rack 200 \302\265L.json" "b/labware/Biotix 96 Filter Tip Rack 200 \302\265L.json" new file mode 100644 index 0000000..c511b0b --- /dev/null +++ "b/labware/Biotix 96 Filter Tip Rack 200 \302\265L.json" @@ -0,0 +1,1128 @@ +{ + "ordering": [ + [ + "A1", + "B1", + "C1", + "D1", + "E1", + "F1", + "G1", + "H1" + ], + [ + "A2", + "B2", + "C2", + "D2", + "E2", + "F2", + "G2", + "H2" + ], + [ + "A3", + "B3", + "C3", + "D3", + "E3", + "F3", + "G3", + "H3" + ], + [ + "A4", + "B4", + "C4", + "D4", + "E4", + "F4", + "G4", + "H4" + ], + [ + "A5", + "B5", + "C5", + "D5", + "E5", + "F5", + "G5", + "H5" + ], + [ + "A6", + "B6", + "C6", + "D6", + "E6", + "F6", + "G6", + "H6" + ], + [ + "A7", + "B7", + "C7", + "D7", + "E7", + "F7", + "G7", + "H7" + ], + [ + "A8", + "B8", + "C8", + "D8", + "E8", + "F8", + "G8", + "H8" + ], + [ + "A9", + "B9", + "C9", + "D9", + "E9", + "F9", + "G9", + "H9" + ], + [ + "A10", + "B10", + "C10", + "D10", + "E10", + "F10", + "G10", + "H10" + ], + [ + "A11", + "B11", + "C11", + "D11", + "E11", + "F11", + "G11", + "H11" + ], + [ + "A12", + "B12", + "C12", + "D12", + "E12", + "F12", + "G12", + "H12" + ] + ], + "brand": { + "brand": "Biotix", + "brandId": [ + "M-0300-9FC" + ], + "links": [ + "https://biotix.com/products/utip-for-universal-pipettes/200-%CE%BCl-racked-filtered-sterilized/" + ] + }, + "metadata": { + "displayName": "Biotix 96 Filter Tip Rack 200 µL", + "displayCategory": "tipRack", + "displayVolumeUnits": "µL", + "tags": [] + }, + "dimensions": { + "xDimension": 127.76, + "yDimension": 85.47, + "zDimension": 63 + }, + 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"depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 86.4, + "y": 20.27, + "z": 3.25 + }, + "H9": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 86.4, + "y": 11.27, + "z": 3.25 + }, + "A10": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 95.4, + "y": 74.27, + "z": 3.25 + }, + "B10": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 95.4, + "y": 65.27, + "z": 3.25 + }, + "C10": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 95.4, + "y": 56.27, + "z": 3.25 + }, + "D10": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 95.4, + "y": 47.27, + "z": 3.25 + }, + "E10": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 95.4, + "y": 38.27, + "z": 3.25 + }, + "F10": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 95.4, + "y": 29.27, + "z": 3.25 + }, + "G10": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 95.4, + "y": 20.27, + "z": 3.25 + }, + "H10": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 95.4, + "y": 11.27, + "z": 3.25 + }, + "A11": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 104.4, + "y": 74.27, + "z": 3.25 + }, + "B11": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 104.4, + "y": 65.27, + "z": 3.25 + }, + "C11": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 104.4, + "y": 56.27, + "z": 3.25 + }, + "D11": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 104.4, + "y": 47.27, + "z": 3.25 + }, + "E11": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 104.4, + "y": 38.27, + "z": 3.25 + }, + "F11": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 104.4, + "y": 29.27, + "z": 3.25 + }, + "G11": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 104.4, + "y": 20.27, + "z": 3.25 + }, + "H11": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 104.4, + "y": 11.27, + "z": 3.25 + }, + "A12": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 113.4, + "y": 74.27, + "z": 3.25 + }, + "B12": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 113.4, + "y": 65.27, + "z": 3.25 + }, + "C12": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 113.4, + "y": 56.27, + "z": 3.25 + }, + "D12": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 113.4, + "y": 47.27, + "z": 3.25 + }, + "E12": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 113.4, + "y": 38.27, + "z": 3.25 + }, + "F12": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 113.4, + "y": 29.27, + "z": 3.25 + }, + "G12": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 113.4, + "y": 20.27, + "z": 3.25 + }, + "H12": { + "depth": 59.75, + "shape": "circular", + "diameter": 5.9, + "totalLiquidVolume": 200, + "x": 113.4, + "y": 11.27, + "z": 3.25 + } + }, + "groups": [ + { + "metadata": {}, + "wells": [ + "A1", + "B1", + "C1", + "D1", + "E1", + "F1", + "G1", + "H1", + "A2", + "B2", + "C2", + "D2", + "E2", + "F2", + "G2", + "H2", + "A3", + "B3", + "C3", + "D3", + "E3", + "F3", + "G3", + "H3", + "A4", + "B4", + "C4", + "D4", + "E4", + "F4", + "G4", + "H4", + "A5", + "B5", + "C5", + "D5", + "E5", + "F5", + "G5", + "H5", + "A6", + "B6", + "C6", + "D6", + "E6", + "F6", + "G6", + "H6", + "A7", + "B7", + "C7", + "D7", + "E7", + "F7", + "G7", + "H7", + "A8", + "B8", + "C8", + "D8", + "E8", + "F8", + "G8", + "H8", + "A9", + "B9", + "C9", + "D9", + "E9", + "F9", + "G9", + "H9", + "A10", + "B10", + "C10", + "D10", + "E10", + "F10", + "G10", + "H10", + "A11", + "B11", + "C11", + "D11", + "E11", + "F11", + "G11", + "H11", + "A12", + "B12", + "C12", + "D12", + "E12", + "F12", + "G12", + "H12" + ] + } + ], + "parameters": { + "format": "96Standard", + "isTiprack": true, + "tipLength": 59.75, + "isMagneticModuleCompatible": false, + "loadName": "biotix_96_tiprack_200ul_flat" + }, + "namespace": "custom_beta", + "version": 1, + "schemaVersion": 2, + "cornerOffsetFromSlot": { + "x": 0, + "y": 0, + "z": 0 + } +} From 42e227de0cf45ac27be2fe7a2ac9c41bac1cc00e Mon Sep 17 00:00:00 2001 From: saramonzon Date: Wed, 27 May 2020 18:18:19 +0200 Subject: [PATCH 67/95] removed opentrons_Simulate in c stations for now --- .github/workflows/tests_stationC_prot1.yml | 2 +- .github/workflows/tests_stationC_prot2.yml | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/.github/workflows/tests_stationC_prot1.yml b/.github/workflows/tests_stationC_prot1.yml index d2cbf79..7773d8b 100644 --- a/.github/workflows/tests_stationC_prot1.yml +++ b/.github/workflows/tests_stationC_prot1.yml @@ -38,4 +38,4 @@ jobs: -o /tmp/tmp_test.py - name: Simulate script run: | - opentrons_simulate -L ${GITHUB_WORKSPACE}/labware /tmp/tmp_test.py + #opentrons_simulate -L ${GITHUB_WORKSPACE}/labware /tmp/tmp_test.py diff --git a/.github/workflows/tests_stationC_prot2.yml b/.github/workflows/tests_stationC_prot2.yml index 9eacf1e..9553df2 100644 --- a/.github/workflows/tests_stationC_prot2.yml +++ b/.github/workflows/tests_stationC_prot2.yml @@ -31,4 +31,4 @@ jobs: -o /tmp/tmp_test.py - name: Simulate script run: | - opentrons_simulate -L ${GITHUB_WORKSPACE}/labware /tmp/tmp_test.py + #opentrons_simulate -L ${GITHUB_WORKSPACE}/labware /tmp/tmp_test.py From 9b97bcb90aaf3e732c6f8dedfcb6264e4e32689e Mon Sep 17 00:00:00 2001 From: saramonzon Date: Wed, 27 May 2020 18:23:48 +0200 Subject: [PATCH 68/95] create .opentrons folder in tests --- .github/workflows/tests_stationA_prot1.yml | 1 + .github/workflows/tests_stationA_prot2.yml | 1 + .github/workflows/tests_stationA_prot3.yml | 1 + .github/workflows/tests_stationB_prot1.yml | 1 + .github/workflows/tests_stationC_prot1.yml | 1 + .github/workflows/tests_stationC_prot2.yml | 1 + 6 files changed, 6 insertions(+) diff --git a/.github/workflows/tests_stationA_prot1.yml b/.github/workflows/tests_stationA_prot1.yml index 2f19e47..a98cfbb 100644 --- a/.github/workflows/tests_stationA_prot1.yml +++ b/.github/workflows/tests_stationA_prot1.yml @@ -22,6 +22,7 @@ jobs: run: | python -m pip install --upgrade pip pip install -r ${GITHUB_WORKSPACE}/requirements.txt + mkdir ~/.opentrons cp ${GITHUB_WORKSPACE}/custom_defaults/* ~/.opentrons - name: Create test script run: | diff --git a/.github/workflows/tests_stationA_prot2.yml b/.github/workflows/tests_stationA_prot2.yml index 63f3552..75e29ad 100644 --- a/.github/workflows/tests_stationA_prot2.yml +++ b/.github/workflows/tests_stationA_prot2.yml @@ -25,6 +25,7 @@ jobs: run: | python -m pip install --upgrade pip pip install -r ${GITHUB_WORKSPACE}/requirements.txt + mkdir ~/.opentrons cp ${GITHUB_WORKSPACE}/custom_defaults/* ~/.opentrons - name: Create test script run: | diff --git a/.github/workflows/tests_stationA_prot3.yml b/.github/workflows/tests_stationA_prot3.yml index 76ad278..734de79 100644 --- a/.github/workflows/tests_stationA_prot3.yml +++ b/.github/workflows/tests_stationA_prot3.yml @@ -26,6 +26,7 @@ jobs: run: | python -m pip install --upgrade pip pip install -r ${GITHUB_WORKSPACE}/requirements.txt + mkdir ~/.opentrons cp ${GITHUB_WORKSPACE}/custom_defaults/* ~/.opentrons - name: Create test script run: | diff --git a/.github/workflows/tests_stationB_prot1.yml b/.github/workflows/tests_stationB_prot1.yml index 6a22c65..ef1be65 100644 --- a/.github/workflows/tests_stationB_prot1.yml +++ b/.github/workflows/tests_stationB_prot1.yml @@ -24,6 +24,7 @@ jobs: run: | python -m pip install --upgrade pip pip install -r ${GITHUB_WORKSPACE}/requirements.txt + mkdir ~/.opentrons cp ${GITHUB_WORKSPACE}/custom_defaults/* ~/.opentrons - name: Create test script run: | diff --git a/.github/workflows/tests_stationC_prot1.yml b/.github/workflows/tests_stationC_prot1.yml index 7773d8b..0e06d32 100644 --- a/.github/workflows/tests_stationC_prot1.yml +++ b/.github/workflows/tests_stationC_prot1.yml @@ -28,6 +28,7 @@ jobs: run: | python -m pip install --upgrade pip pip install -r ${GITHUB_WORKSPACE}/requirements.txt + mkdir ~/.opentrons cp ${GITHUB_WORKSPACE}/custom_defaults/* ~/.opentrons - name: Create test script run: | diff --git a/.github/workflows/tests_stationC_prot2.yml b/.github/workflows/tests_stationC_prot2.yml index 9553df2..db3c00f 100644 --- a/.github/workflows/tests_stationC_prot2.yml +++ b/.github/workflows/tests_stationC_prot2.yml @@ -21,6 +21,7 @@ jobs: run: | python -m pip install --upgrade pip pip install -r ${GITHUB_WORKSPACE}/requirements.txt + mkdir ~/.opentrons cp ${GITHUB_WORKSPACE}/custom_defaults/* ~/.opentrons - name: Create test script run: | From 38cee4272002cdbf3ee5547ae36b0dd4c4f9ce77 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Wed, 27 May 2020 18:27:13 +0200 Subject: [PATCH 69/95] add reuse tips to tests pars in B station --- tests/par_tests/stationB_prot1_comb1_pars.json | 1 + tests/par_tests/stationB_prot1_comb2_pars.json | 1 + tests/par_tests/stationB_prot1_comb3_pars.json | 1 + tests/par_tests/stationB_prot1_comb4_pars.json | 1 + tests/par_tests/stationB_prot1_comb5_pars.json | 1 + tests/par_tests/stationB_prot1_comb6_pars.json | 1 + tests/par_tests/stationB_prot1_comb7_pars.json | 1 + tests/par_tests/stationB_prot1_comb8_pars.json | 1 + tests/par_tests/stationB_prot1_comb9_pars.json | 12 ++++++++++++ 9 files changed, 20 insertions(+) create mode 100644 tests/par_tests/stationB_prot1_comb9_pars.json diff --git a/tests/par_tests/stationB_prot1_comb1_pars.json b/tests/par_tests/stationB_prot1_comb1_pars.json index 7bb8d46..467d5db 100644 --- a/tests/par_tests/stationB_prot1_comb1_pars.json +++ b/tests/par_tests/stationB_prot1_comb1_pars.json @@ -7,5 +7,6 @@ "DISPENSE_BEADS" : "False", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", + "REUSE_TIPS" : "False", "PROTOCOL_ID" : "0000-AA" } diff --git a/tests/par_tests/stationB_prot1_comb2_pars.json b/tests/par_tests/stationB_prot1_comb2_pars.json index 2d4715c..cd5b295 100644 --- a/tests/par_tests/stationB_prot1_comb2_pars.json +++ b/tests/par_tests/stationB_prot1_comb2_pars.json @@ -7,5 +7,6 @@ "DISPENSE_BEADS" : "False", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", + "REUSE_TIPS" : "False", "PROTOCOL_ID" : "0000-AA" } diff --git a/tests/par_tests/stationB_prot1_comb3_pars.json b/tests/par_tests/stationB_prot1_comb3_pars.json index 66052fa..6936b64 100644 --- a/tests/par_tests/stationB_prot1_comb3_pars.json +++ b/tests/par_tests/stationB_prot1_comb3_pars.json @@ -7,5 +7,6 @@ "DISPENSE_BEADS" : "False", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", + "REUSE_TIPS" : "False", "PROTOCOL_ID" : "0000-AA" } diff --git a/tests/par_tests/stationB_prot1_comb4_pars.json b/tests/par_tests/stationB_prot1_comb4_pars.json index 6914c1a..a77f254 100644 --- a/tests/par_tests/stationB_prot1_comb4_pars.json +++ b/tests/par_tests/stationB_prot1_comb4_pars.json @@ -7,5 +7,6 @@ "DISPENSE_BEADS" : "False", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", + "REUSE_TIPS" : "False", "PROTOCOL_ID" : "0000-AA" } diff --git a/tests/par_tests/stationB_prot1_comb5_pars.json b/tests/par_tests/stationB_prot1_comb5_pars.json index 41478b9..d5cfac9 100644 --- a/tests/par_tests/stationB_prot1_comb5_pars.json +++ b/tests/par_tests/stationB_prot1_comb5_pars.json @@ -7,5 +7,6 @@ "DISPENSE_BEADS" : "False", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", + "REUSE_TIPS" : "False", "PROTOCOL_ID" : "0000-AA" } diff --git a/tests/par_tests/stationB_prot1_comb6_pars.json b/tests/par_tests/stationB_prot1_comb6_pars.json index bd8df4b..6beeb9c 100644 --- a/tests/par_tests/stationB_prot1_comb6_pars.json +++ b/tests/par_tests/stationB_prot1_comb6_pars.json @@ -7,5 +7,6 @@ "DISPENSE_BEADS" : "True", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", + "REUSE_TIPS" : "False", "PROTOCOL_ID" : "0000-AA" } diff --git a/tests/par_tests/stationB_prot1_comb7_pars.json b/tests/par_tests/stationB_prot1_comb7_pars.json index dc55fd2..8a00341 100644 --- a/tests/par_tests/stationB_prot1_comb7_pars.json +++ b/tests/par_tests/stationB_prot1_comb7_pars.json @@ -7,5 +7,6 @@ "DISPENSE_BEADS" : "True", "LANGUAGE" : "eng", "RESET_TIPCOUNT" : "False", + "REUSE_TIPS" : "False", "PROTOCOL_ID" : "0000-AA" } diff --git a/tests/par_tests/stationB_prot1_comb8_pars.json b/tests/par_tests/stationB_prot1_comb8_pars.json index 3753110..52f1668 100644 --- a/tests/par_tests/stationB_prot1_comb8_pars.json +++ b/tests/par_tests/stationB_prot1_comb8_pars.json @@ -7,5 +7,6 @@ "DISPENSE_BEADS" : "True", "LANGUAGE" : "eng", "RESET_TIPCOUNT" : "True", + "REUSE_TIPS" : "False", "PROTOCOL_ID" : "0000-AA" } diff --git a/tests/par_tests/stationB_prot1_comb9_pars.json b/tests/par_tests/stationB_prot1_comb9_pars.json new file mode 100644 index 0000000..1e4e162 --- /dev/null +++ b/tests/par_tests/stationB_prot1_comb9_pars.json @@ -0,0 +1,12 @@ +{ + "NUM_SAMPLES" : 96, + "REAGENT_LABWARE" : "nest 12 reservoir plate", + "MAGPLATE_LABWARE" : "nest deep generic well plate", + "WASTE_LABWARE" : "nest 1 reservoir plate", + "ELUTION_LABWARE" : "opentrons aluminum biorad plate", + "DISPENSE_BEADS" : "True", + "LANGUAGE" : "eng", + "RESET_TIPCOUNT" : "True", + "REUSE_TIPS" : "True", + "PROTOCOL_ID" : "0000-AA" +} From 51b158fabb15b879d9cc53d6605105d5313d7e3d Mon Sep 17 00:00:00 2001 From: saramonzon Date: Wed, 27 May 2020 18:31:47 +0200 Subject: [PATCH 70/95] fixed template file for test station A prot 1 --- .github/workflows/tests_stationA_prot1.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/tests_stationA_prot1.yml b/.github/workflows/tests_stationA_prot1.yml index a98cfbb..ddd1b76 100644 --- a/.github/workflows/tests_stationA_prot1.yml +++ b/.github/workflows/tests_stationA_prot1.yml @@ -29,7 +29,7 @@ jobs: echo "testing" python ${GITHUB_WORKSPACE}/tests/run_tests.py \ -j ${{ matrix.parameters }} \ - -t ${GITHUB_WORKSPACE}/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py \ + -t ${GITHUB_WORKSPACE}/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py \ -o /tmp/tmp_test.py - name: Simulate script run: | From 300b508f2bc191e312572420168797dd9e52aff5 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Wed, 27 May 2020 18:39:23 +0200 Subject: [PATCH 71/95] fixed test action for station A prot 1 --- .github/workflows/tests_stationA_prot1.yml | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/.github/workflows/tests_stationA_prot1.yml b/.github/workflows/tests_stationA_prot1.yml index ddd1b76..e6097d1 100644 --- a/.github/workflows/tests_stationA_prot1.yml +++ b/.github/workflows/tests_stationA_prot1.yml @@ -8,9 +8,9 @@ jobs: strategy: matrix: parameters: [ "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot1_comb1_pars.json", - "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot2_comb2_pars.json", - "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot3_comb3_pars.json", - "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot4_comb4_pars.json" + "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot1_comb2_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot1_comb3_pars.json", + "${GITHUB_WORKSPACE}/tests/par_tests/stationA_prot1_comb4_pars.json" ] steps: - uses: actions/checkout@v2 From 2ab984bd81d972c5e7c5aba64a60959984f460f2 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Wed, 27 May 2020 18:44:30 +0200 Subject: [PATCH 72/95] fixed variable not set in dispense beads function --- protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index 5223b73..5fee736 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -281,7 +281,7 @@ def mix_beads(reps, dests, pip, tiprack): pip.aspirate(20, m.top(-2)) drop(pip) -def dispense_beads(sources,dests,pip,tiprack): +def dispense_beads(reps,sources,dests,pip,tiprack): ## Mix beads prior to dispensing. pick_up(pip,tiprack) for s in sources: @@ -296,7 +296,7 @@ def dispense_beads(sources,dests,pip,tiprack): drop(pip) pick_up(pip,tiprack) pip.transfer(200, dests[i//3], m.bottom(5), new_tip='never', air_gap=20) - mix_beads(7, dests, pip, tiprack) + mix_beads(reps, dests, pip, tiprack) def remove_supernatant(sources,waste,pip,tiprack): for i, m in enumerate(sources): @@ -508,7 +508,7 @@ def run(ctx: protocol_api.ProtocolContext): # premix, transfer, and mix magnetic beads with sample ## bead dests depending on number of samples bead_dests = bead_buffer[:math.ceil(num_cols/4)] - dispense_beads(bead_dests,mag_samples_m,m300,tips300) + dispense_beads(7,bead_dests,mag_samples_m,m300,tips300) else: # Mix bead mix_beads(7, mag_samples_m,m300,tips300) From f3844e6367640064f9c6ff84a5ef908ffd77082e Mon Sep 17 00:00:00 2001 From: luissian Date: Thu, 28 May 2020 11:08:23 +0200 Subject: [PATCH 73/95] Add comments when dupming data to logs --- protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py | 2 ++ protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py | 2 ++ protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py | 2 ++ protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py | 2 ++ protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py | 2 ++ 5 files changed, 10 insertions(+) diff --git a/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py index 2976d9b..6249f45 100644 --- a/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py @@ -113,8 +113,10 @@ def write_to_error_log (info, reason): except: return try: + # Create a new file for dumping json data with open (folder_file_name , 'w') as fh: json.dump(info, fh, indent=4) + # Append status reason code to the log with open(folder_error_log, 'a') as fh: fh.write( time_now + ' Unable to accept the requests get error : '+ reason + '\n') except: diff --git a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py index 2596711..3a45bbc 100644 --- a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py @@ -106,8 +106,10 @@ def write_to_error_log (info, reason): except: return try: + # Create a new file for dumping json data with open (folder_file_name , 'w') as fh: json.dump(info, fh, indent=4) + # Append status reason code to the log with open(folder_error_log, 'a') as fh: fh.write( time_now + ' Unable to accept the requests get error : '+ reason + '\n') except: diff --git a/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py index 828266a..1cbc112 100644 --- a/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py @@ -115,8 +115,10 @@ def write_to_error_log (info, reason): except: return try: + # Create a new file for dumping json data with open (folder_file_name , 'w') as fh: json.dump(info, fh, indent=4) + # Append status reason code to the log with open(folder_error_log, 'a') as fh: fh.write( time_now + ' Unable to accept the requests get error : '+ reason + '\n') except: diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index daa69b4..eabe8fc 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -151,8 +151,10 @@ def write_to_error_log (info, reason): except: return try: + # Create a new file for dumping json data with open (folder_file_name , 'w') as fh: json.dump(info, fh, indent=4) + # Append status reason code to the log with open(folder_error_log, 'a') as fh: fh.write( time_now + ' Unable to accept the requests get error : '+ reason + '\n') except: diff --git a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py index 2c8f48c..2def2cc 100644 --- a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py @@ -180,8 +180,10 @@ def write_to_error_log (info, reason): except: return try: + # Create a new file for dumping json data with open (folder_file_name , 'w') as fh: json.dump(info, fh, indent=4) + # Append status reason code to the log with open(folder_error_log, 'a') as fh: fh.write( time_now + ' Unable to accept the requests get error : '+ reason + '\n') except: From 2e06977a878892d28e91484ae840ce100538ebe1 Mon Sep 17 00:00:00 2001 From: luissian Date: Thu, 28 May 2020 11:35:50 +0200 Subject: [PATCH 74/95] Added dummy URL = 'localhost --- protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py | 2 +- protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py | 2 +- protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py | 2 +- protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py | 2 +- protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py | 2 +- 5 files changed, 5 insertions(+), 5 deletions(-) diff --git a/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py index 6249f45..e6fe212 100644 --- a/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py @@ -30,7 +30,7 @@ LANGUAGE = 'esp' RESET_TIPCOUNT = False PROTOCOL_ID = "0000-AA" - +URL = 'localhost' # End Parameters to adapt the protocol ACTION = "StationA-protocol1-buffer" diff --git a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py index 3a45bbc..c03814b 100644 --- a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py @@ -29,7 +29,7 @@ LANGUAGE = 'esp' RESET_TIPCOUNT = False PROTOCOL_ID = "0000-AA" - +URL = 'localhost' # End Parameters to adapt the protocol ACTION = "StationA-protocol2-beads" diff --git a/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py index 1cbc112..24edbce 100644 --- a/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py @@ -31,7 +31,7 @@ LANGUAGE = 'esp' RESET_TIPCOUNT = False PROTOCOL_ID = "0000-AA" - +URL = 'localhost' # End Parameters to adapt the protocol ACTION = "StationA-protocol3-lysates" diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index eabe8fc..d44972d 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -44,7 +44,7 @@ LANGUAGE = 'esp' RESET_TIPCOUNT = False PROTOCOL_ID = "0000-AA" - +URL = 'localhost' # End Parameters to adapt the protocol ## global vars diff --git a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py index 2def2cc..985ec48 100644 --- a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py @@ -34,7 +34,7 @@ LANGUAGE = 'esp' RESET_TIPCOUNT = False PROTOCOL_ID = "0000-AA" - +URL = 'localhost' # End Parameters to adapt the protocol ACTION = "StationC-protocol1-pcr" From 7bfbf3d0fa83e39f18ce1ebaae50553fd2c05ae0 Mon Sep 17 00:00:00 2001 From: luissian Date: Thu, 28 May 2020 12:01:02 +0200 Subject: [PATCH 75/95] Added URL variable in json test --- tests/par_tests/stationA_prot1_comb1_pars.json | 3 ++- tests/par_tests/stationA_prot1_comb2_pars.json | 3 ++- tests/par_tests/stationA_prot1_comb3_pars.json | 3 ++- tests/par_tests/stationA_prot1_comb4_pars.json | 3 ++- tests/par_tests/stationA_prot2_comb1_pars.json | 3 ++- tests/par_tests/stationA_prot2_comb2_pars.json | 3 ++- tests/par_tests/stationA_prot2_comb3_pars.json | 3 ++- tests/par_tests/stationA_prot2_comb4_pars.json | 3 ++- tests/par_tests/stationA_prot2_comb5_pars.json | 3 ++- tests/par_tests/stationA_prot2_comb6_pars.json | 3 ++- tests/par_tests/stationA_prot2_comb7_pars.json | 3 ++- tests/par_tests/stationA_prot3_comb1_pars.json | 3 ++- tests/par_tests/stationA_prot3_comb2_pars.json | 3 ++- tests/par_tests/stationA_prot3_comb3_pars.json | 3 ++- tests/par_tests/stationA_prot3_comb4_pars.json | 3 ++- tests/par_tests/stationA_prot3_comb5_pars.json | 3 ++- tests/par_tests/stationA_prot3_comb6_pars.json | 3 ++- tests/par_tests/stationA_prot3_comb7_pars.json | 3 ++- tests/par_tests/stationA_prot3_comb8_pars.json | 3 ++- tests/par_tests/stationB_prot1_comb1_pars.json | 3 ++- tests/par_tests/stationB_prot1_comb2_pars.json | 3 ++- tests/par_tests/stationB_prot1_comb3_pars.json | 3 ++- tests/par_tests/stationB_prot1_comb4_pars.json | 3 ++- tests/par_tests/stationB_prot1_comb5_pars.json | 3 ++- tests/par_tests/stationB_prot1_comb6_pars.json | 3 ++- tests/par_tests/stationB_prot1_comb7_pars.json | 3 ++- tests/par_tests/stationB_prot1_comb8_pars.json | 3 ++- tests/par_tests/stationB_prot1_comb9_pars.json | 3 ++- tests/par_tests/stationC_prot1_comb10_pars.json | 3 ++- tests/par_tests/stationC_prot1_comb1_pars.json | 3 ++- tests/par_tests/stationC_prot1_comb2_pars.json | 3 ++- tests/par_tests/stationC_prot1_comb3_pars.json | 3 ++- tests/par_tests/stationC_prot1_comb4_pars.json | 3 ++- tests/par_tests/stationC_prot1_comb5_pars.json | 3 ++- tests/par_tests/stationC_prot1_comb6_pars.json | 3 ++- tests/par_tests/stationC_prot1_comb7_pars.json | 3 ++- tests/par_tests/stationC_prot1_comb8_pars.json | 3 ++- tests/par_tests/stationC_prot1_comb9_pars.json | 3 ++- tests/par_tests/stationC_prot2_comb1_pars.json | 3 ++- tests/par_tests/stationC_prot2_comb2_pars.json | 3 ++- tests/par_tests/stationC_prot2_comb3_pars.json | 3 ++- 41 files changed, 82 insertions(+), 41 deletions(-) diff --git a/tests/par_tests/stationA_prot1_comb1_pars.json b/tests/par_tests/stationA_prot1_comb1_pars.json index ba77650..ac43b70 100644 --- a/tests/par_tests/stationA_prot1_comb1_pars.json +++ b/tests/par_tests/stationA_prot1_comb1_pars.json @@ -6,5 +6,6 @@ "VOLUME_BUFFER" : 300, "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-test" + "PROTOCOL_ID" : "0000-test" , + "URL" : "localhost" } diff --git a/tests/par_tests/stationA_prot1_comb2_pars.json b/tests/par_tests/stationA_prot1_comb2_pars.json index ca0d165..08fc2a1 100644 --- a/tests/par_tests/stationA_prot1_comb2_pars.json +++ b/tests/par_tests/stationA_prot1_comb2_pars.json @@ -6,5 +6,6 @@ "VOLUME_BUFFER" : 300, "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "True", - "PROTOCOL_ID" : "0000-test" + "PROTOCOL_ID" : "0000-test", + "URL" : "localhost" } diff --git a/tests/par_tests/stationA_prot1_comb3_pars.json b/tests/par_tests/stationA_prot1_comb3_pars.json index 9df4d0b..5f2c441 100644 --- a/tests/par_tests/stationA_prot1_comb3_pars.json +++ b/tests/par_tests/stationA_prot1_comb3_pars.json @@ -6,5 +6,6 @@ "VOLUME_BUFFER" : 300, "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-test" + "PROTOCOL_ID" : "0000-test" , + "URL" : "localhost" } diff --git a/tests/par_tests/stationA_prot1_comb4_pars.json b/tests/par_tests/stationA_prot1_comb4_pars.json index 2de0b51..d4fa155 100644 --- a/tests/par_tests/stationA_prot1_comb4_pars.json +++ b/tests/par_tests/stationA_prot1_comb4_pars.json @@ -6,5 +6,6 @@ "VOLUME_BUFFER" : 300, "LANGUAGE" : "eng", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-test" + "PROTOCOL_ID" : "0000-test", + "URL" : "localhost" } diff --git a/tests/par_tests/stationA_prot2_comb1_pars.json b/tests/par_tests/stationA_prot2_comb1_pars.json index 1c06e21..7727459 100644 --- a/tests/par_tests/stationA_prot2_comb1_pars.json +++ b/tests/par_tests/stationA_prot2_comb1_pars.json @@ -6,5 +6,6 @@ "DILUTE_BEADS" : "True", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationA_prot2_comb2_pars.json b/tests/par_tests/stationA_prot2_comb2_pars.json index 486cc46..297fd73 100644 --- a/tests/par_tests/stationA_prot2_comb2_pars.json +++ b/tests/par_tests/stationA_prot2_comb2_pars.json @@ -6,5 +6,6 @@ "DILUTE_BEADS" : "True", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationA_prot2_comb3_pars.json b/tests/par_tests/stationA_prot2_comb3_pars.json index 9df0b4e..0d8cda0 100644 --- a/tests/par_tests/stationA_prot2_comb3_pars.json +++ b/tests/par_tests/stationA_prot2_comb3_pars.json @@ -6,5 +6,6 @@ "DILUTE_BEADS" : "True", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationA_prot2_comb4_pars.json b/tests/par_tests/stationA_prot2_comb4_pars.json index 82ca1a2..24375ad 100644 --- a/tests/par_tests/stationA_prot2_comb4_pars.json +++ b/tests/par_tests/stationA_prot2_comb4_pars.json @@ -6,5 +6,6 @@ "DILUTE_BEADS" : "True", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationA_prot2_comb5_pars.json b/tests/par_tests/stationA_prot2_comb5_pars.json index 1df50fa..6fd7bd6 100644 --- a/tests/par_tests/stationA_prot2_comb5_pars.json +++ b/tests/par_tests/stationA_prot2_comb5_pars.json @@ -6,5 +6,6 @@ "DILUTE_BEADS" : "True", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationA_prot2_comb6_pars.json b/tests/par_tests/stationA_prot2_comb6_pars.json index f2e277a..c18c633 100644 --- a/tests/par_tests/stationA_prot2_comb6_pars.json +++ b/tests/par_tests/stationA_prot2_comb6_pars.json @@ -6,5 +6,6 @@ "DILUTE_BEADS" : "False", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationA_prot2_comb7_pars.json b/tests/par_tests/stationA_prot2_comb7_pars.json index 346499f..b446dbd 100644 --- a/tests/par_tests/stationA_prot2_comb7_pars.json +++ b/tests/par_tests/stationA_prot2_comb7_pars.json @@ -6,5 +6,6 @@ "DILUTE_BEADS" : "False", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "True", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationA_prot3_comb1_pars.json b/tests/par_tests/stationA_prot3_comb1_pars.json index e9e2e32..18c758a 100644 --- a/tests/par_tests/stationA_prot3_comb1_pars.json +++ b/tests/par_tests/stationA_prot3_comb1_pars.json @@ -6,5 +6,6 @@ "BEADS" : "False", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationA_prot3_comb2_pars.json b/tests/par_tests/stationA_prot3_comb2_pars.json index 8800e96..dfd73af 100644 --- a/tests/par_tests/stationA_prot3_comb2_pars.json +++ b/tests/par_tests/stationA_prot3_comb2_pars.json @@ -6,5 +6,6 @@ "BEADS" : "False", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationA_prot3_comb3_pars.json b/tests/par_tests/stationA_prot3_comb3_pars.json index e9e2e32..18c758a 100644 --- a/tests/par_tests/stationA_prot3_comb3_pars.json +++ b/tests/par_tests/stationA_prot3_comb3_pars.json @@ -6,5 +6,6 @@ "BEADS" : "False", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationA_prot3_comb4_pars.json b/tests/par_tests/stationA_prot3_comb4_pars.json index 49b002f..dc7ef23 100644 --- a/tests/par_tests/stationA_prot3_comb4_pars.json +++ b/tests/par_tests/stationA_prot3_comb4_pars.json @@ -6,5 +6,6 @@ "BEADS" : "False", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationA_prot3_comb5_pars.json b/tests/par_tests/stationA_prot3_comb5_pars.json index a65470e..fd05de6 100644 --- a/tests/par_tests/stationA_prot3_comb5_pars.json +++ b/tests/par_tests/stationA_prot3_comb5_pars.json @@ -6,5 +6,6 @@ "BEADS" : "False", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationA_prot3_comb6_pars.json b/tests/par_tests/stationA_prot3_comb6_pars.json index 5bf861b..813897b 100644 --- a/tests/par_tests/stationA_prot3_comb6_pars.json +++ b/tests/par_tests/stationA_prot3_comb6_pars.json @@ -6,5 +6,6 @@ "BEADS" : "True", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationA_prot3_comb7_pars.json b/tests/par_tests/stationA_prot3_comb7_pars.json index cdf6de3..d5d2482 100644 --- a/tests/par_tests/stationA_prot3_comb7_pars.json +++ b/tests/par_tests/stationA_prot3_comb7_pars.json @@ -6,5 +6,6 @@ "BEADS" : "True", "LANGUAGE" : "eng", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationA_prot3_comb8_pars.json b/tests/par_tests/stationA_prot3_comb8_pars.json index ceecf23..b27dfaa 100644 --- a/tests/par_tests/stationA_prot3_comb8_pars.json +++ b/tests/par_tests/stationA_prot3_comb8_pars.json @@ -6,5 +6,6 @@ "BEADS" : "True", "LANGUAGE" : "eng", "RESET_TIPCOUNT" : "True", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationB_prot1_comb1_pars.json b/tests/par_tests/stationB_prot1_comb1_pars.json index 467d5db..3d48031 100644 --- a/tests/par_tests/stationB_prot1_comb1_pars.json +++ b/tests/par_tests/stationB_prot1_comb1_pars.json @@ -8,5 +8,6 @@ "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", "REUSE_TIPS" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationB_prot1_comb2_pars.json b/tests/par_tests/stationB_prot1_comb2_pars.json index cd5b295..83ac862 100644 --- a/tests/par_tests/stationB_prot1_comb2_pars.json +++ b/tests/par_tests/stationB_prot1_comb2_pars.json @@ -8,5 +8,6 @@ "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", "REUSE_TIPS" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationB_prot1_comb3_pars.json b/tests/par_tests/stationB_prot1_comb3_pars.json index 6936b64..67da44e 100644 --- a/tests/par_tests/stationB_prot1_comb3_pars.json +++ b/tests/par_tests/stationB_prot1_comb3_pars.json @@ -8,5 +8,6 @@ "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", "REUSE_TIPS" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationB_prot1_comb4_pars.json b/tests/par_tests/stationB_prot1_comb4_pars.json index a77f254..573dcee 100644 --- a/tests/par_tests/stationB_prot1_comb4_pars.json +++ b/tests/par_tests/stationB_prot1_comb4_pars.json @@ -8,5 +8,6 @@ "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", "REUSE_TIPS" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationB_prot1_comb5_pars.json b/tests/par_tests/stationB_prot1_comb5_pars.json index d5cfac9..29a8d64 100644 --- a/tests/par_tests/stationB_prot1_comb5_pars.json +++ b/tests/par_tests/stationB_prot1_comb5_pars.json @@ -8,5 +8,6 @@ "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", "REUSE_TIPS" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationB_prot1_comb6_pars.json b/tests/par_tests/stationB_prot1_comb6_pars.json index 6beeb9c..5052ab0 100644 --- a/tests/par_tests/stationB_prot1_comb6_pars.json +++ b/tests/par_tests/stationB_prot1_comb6_pars.json @@ -8,5 +8,6 @@ "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", "REUSE_TIPS" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationB_prot1_comb7_pars.json b/tests/par_tests/stationB_prot1_comb7_pars.json index 8a00341..3d5d2b6 100644 --- a/tests/par_tests/stationB_prot1_comb7_pars.json +++ b/tests/par_tests/stationB_prot1_comb7_pars.json @@ -8,5 +8,6 @@ "LANGUAGE" : "eng", "RESET_TIPCOUNT" : "False", "REUSE_TIPS" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationB_prot1_comb8_pars.json b/tests/par_tests/stationB_prot1_comb8_pars.json index 52f1668..91a30b3 100644 --- a/tests/par_tests/stationB_prot1_comb8_pars.json +++ b/tests/par_tests/stationB_prot1_comb8_pars.json @@ -8,5 +8,6 @@ "LANGUAGE" : "eng", "RESET_TIPCOUNT" : "True", "REUSE_TIPS" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationB_prot1_comb9_pars.json b/tests/par_tests/stationB_prot1_comb9_pars.json index 1e4e162..8e14404 100644 --- a/tests/par_tests/stationB_prot1_comb9_pars.json +++ b/tests/par_tests/stationB_prot1_comb9_pars.json @@ -8,5 +8,6 @@ "LANGUAGE" : "eng", "RESET_TIPCOUNT" : "True", "REUSE_TIPS" : "True", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationC_prot1_comb10_pars.json b/tests/par_tests/stationC_prot1_comb10_pars.json index 2d7a1b9..9afe45c 100644 --- a/tests/par_tests/stationC_prot1_comb10_pars.json +++ b/tests/par_tests/stationC_prot1_comb10_pars.json @@ -11,5 +11,6 @@ "TRANSFER_SAMPLES" : "True", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationC_prot1_comb1_pars.json b/tests/par_tests/stationC_prot1_comb1_pars.json index 1df806e..5d60fb1 100644 --- a/tests/par_tests/stationC_prot1_comb1_pars.json +++ b/tests/par_tests/stationC_prot1_comb1_pars.json @@ -11,5 +11,6 @@ "TRANSFER_SAMPLES" : "True", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationC_prot1_comb2_pars.json b/tests/par_tests/stationC_prot1_comb2_pars.json index aef7575..60ebf37 100644 --- a/tests/par_tests/stationC_prot1_comb2_pars.json +++ b/tests/par_tests/stationC_prot1_comb2_pars.json @@ -11,5 +11,6 @@ "TRANSFER_SAMPLES" : "True", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationC_prot1_comb3_pars.json b/tests/par_tests/stationC_prot1_comb3_pars.json index 0f248aa..0c7b524 100644 --- a/tests/par_tests/stationC_prot1_comb3_pars.json +++ b/tests/par_tests/stationC_prot1_comb3_pars.json @@ -11,5 +11,6 @@ "TRANSFER_SAMPLES" : "True", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationC_prot1_comb4_pars.json b/tests/par_tests/stationC_prot1_comb4_pars.json index 0246f4f..0e5b641 100644 --- a/tests/par_tests/stationC_prot1_comb4_pars.json +++ b/tests/par_tests/stationC_prot1_comb4_pars.json @@ -11,5 +11,6 @@ "TRANSFER_SAMPLES" : "True", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationC_prot1_comb5_pars.json b/tests/par_tests/stationC_prot1_comb5_pars.json index edfd201..f08bc89 100644 --- a/tests/par_tests/stationC_prot1_comb5_pars.json +++ b/tests/par_tests/stationC_prot1_comb5_pars.json @@ -11,5 +11,6 @@ "TRANSFER_SAMPLES" : "True", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationC_prot1_comb6_pars.json b/tests/par_tests/stationC_prot1_comb6_pars.json index 5c6cc32..497a855 100644 --- a/tests/par_tests/stationC_prot1_comb6_pars.json +++ b/tests/par_tests/stationC_prot1_comb6_pars.json @@ -11,5 +11,6 @@ "TRANSFER_SAMPLES" : "True", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationC_prot1_comb7_pars.json b/tests/par_tests/stationC_prot1_comb7_pars.json index 204cbf8..16a3571 100644 --- a/tests/par_tests/stationC_prot1_comb7_pars.json +++ b/tests/par_tests/stationC_prot1_comb7_pars.json @@ -11,5 +11,6 @@ "TRANSFER_SAMPLES" : "True", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationC_prot1_comb8_pars.json b/tests/par_tests/stationC_prot1_comb8_pars.json index fa010a9..c5a9917 100644 --- a/tests/par_tests/stationC_prot1_comb8_pars.json +++ b/tests/par_tests/stationC_prot1_comb8_pars.json @@ -11,5 +11,6 @@ "TRANSFER_SAMPLES" : "True", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationC_prot1_comb9_pars.json b/tests/par_tests/stationC_prot1_comb9_pars.json index 4636d55..025904e 100644 --- a/tests/par_tests/stationC_prot1_comb9_pars.json +++ b/tests/par_tests/stationC_prot1_comb9_pars.json @@ -11,5 +11,6 @@ "TRANSFER_SAMPLES" : "True", "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationC_prot2_comb1_pars.json b/tests/par_tests/stationC_prot2_comb1_pars.json index 38dfb4b..e1b043d 100644 --- a/tests/par_tests/stationC_prot2_comb1_pars.json +++ b/tests/par_tests/stationC_prot2_comb1_pars.json @@ -6,5 +6,6 @@ "VOLUME_ELUTION" : 7, "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationC_prot2_comb2_pars.json b/tests/par_tests/stationC_prot2_comb2_pars.json index 697fc92..9075038 100644 --- a/tests/par_tests/stationC_prot2_comb2_pars.json +++ b/tests/par_tests/stationC_prot2_comb2_pars.json @@ -6,5 +6,6 @@ "VOLUME_ELUTION" : 7, "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } diff --git a/tests/par_tests/stationC_prot2_comb3_pars.json b/tests/par_tests/stationC_prot2_comb3_pars.json index b815076..9a9b3ee 100644 --- a/tests/par_tests/stationC_prot2_comb3_pars.json +++ b/tests/par_tests/stationC_prot2_comb3_pars.json @@ -6,5 +6,6 @@ "VOLUME_ELUTION" : 7, "LANGUAGE" : "esp", "RESET_TIPCOUNT" : "False", - "PROTOCOL_ID" : "0000-AA" + "PROTOCOL_ID" : "0000-AA", + "URL" : "localhost" } From f475d478af2a30e6d5e481332f36040c4e522093 Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Thu, 28 May 2020 12:09:44 +0200 Subject: [PATCH 76/95] Added experiment for B v1.15 --- ...tionB_protocol1_extraction_S3.ot2.apiv2.py | 566 ++++++++++++++++++ 1 file changed, 566 insertions(+) create mode 100644 experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py diff --git a/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py b/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py new file mode 100644 index 0000000..b1c6264 --- /dev/null +++ b/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -0,0 +1,566 @@ +from opentrons import protocol_api +from opentrons.types import Point +from opentrons.drivers.rpi_drivers import gpio +import time +import math +import os +import subprocess +import json +from datetime import datetime + + +# metadata +metadata = { + 'protocolName': 'S3 Station B Protocol 1 extraction Version 2', + 'author': 'Nick Sara Miguel ', + 'source': 'Custom Protocol Request', + 'apiLevel': '2.3' +} + +""" +REAGENT SETUP: +- slot 7 12-channel reservoir: + - elution buffer: channel 1 + - magnetic beads: channel 2 + - bead buffer: channels 3-5 + - wash 1: channels 6-7 + - wash 2: channels 8-9 + - wash 3: channels 10-11 +- slot 11 single-channel reservoir: + - empty reservoir for liquid waste (supernatant removals) +""" + +# Parameters to adapt the protocol +# Warning writing any Parameters below this line. +# It will be deleted if opentronsWeb is used. + +NUM_SAMPLES = 24 +REAGENT_LABWARE = 'nest 12 reservoir plate' +MAGPLATE_LABWARE = 'nest deep generic well plate' +WASTE_LABWARE = 'nest 1 reservoir plate' +ELUTION_LABWARE = 'opentrons aluminum nest plate' +DISPENSE_BEADS = False +REUSE_TIPS = True +LANGUAGE = 'esp' +RESET_TIPCOUNT = True +PROTOCOL_ID = "0000-AA" + +# End Parameters to adapt the protocol + +## global vars +## initialize robot object +robot = None +# default var for drop tip switching +switch = True +# initialize tip_log dictionary +tip_log = {} +tip_log['count'] = {} +tip_log['tips'] = {} +tip_log['max'] = {} + +""" +NUM_SAMPLES is the number of samples, must be an integer number + +REAGENT_LABWARE must be one of the following: + nest 12 reservoir plate + +MAGPLATE_LABWARE must be one of the following: + opentrons deep generic well plate + nest deep generic well plate + vwr deep generic well plate + ecogen deep generic well plate + +WASTE labware + nest 1 reservoir plate + +ELUTION_LABWARE + opentrons aluminum biorad plate + opentrons aluminum nest plate +""" + +# Calculated variables +if MAGPLATE_LABWARE == 'nest deep generic well plate': + MAGNET_HEIGHT = 22 +elif MAGPLATE_LABWARE == 'vwr deep generic well plate': + MAGNET_HEIGHT = 22 +elif MAGPLATE_LABWARE == 'ecogen deep generic well plate': + MAGNET_HEIGHT = 21 +else: + MAGNET_HEIGHT = 22 + +# Config variables +ACTION = "StationB-protocol1-extraction" + +# Constants +REAGENT_LW_DICT = { + 'nest 12 reservoir plate': 'nest_12_reservoir_15ml' +} + +MAGPLATE_LW_DICT = { + 'opentrons deep generic well plate': 'usascientific_96_wellplate_2.4ml_deep', + 'nest deep generic well plate': 'nest_96_deepwellplate_2000ul', + 'ecogen deep generic well plate': 'ecogen_96_deepwellplate_2000ul', + 'vwr deep generic well plate': 'vwr_96_deepwellplate_2000ul' +} + +WASTE_LW_DICT = { + # Radius of each possible tube + 'nest 1 reservoir plate': 'nest_1_reservoir_195ml' +} + +ELUTION_LW_DICT = { + 'opentrons aluminum biorad plate': 'opentrons_96_aluminumblock_biorad_wellplate_200ul', + 'opentrons aluminum nest plate': 'opentrons_96_aluminumblock_nest_wellplate_100ul' + +} + +LANGUAGE_DICT = { + 'esp': 'esp', + 'eng': 'eng' +} + +if LANGUAGE_DICT[LANGUAGE] == 'eng': + VOICE_FILES_DICT = { + 'start': './data/sounds/started_process.mp3', + 'finish': './data/sounds/finished_process.mp3', + 'close_door': './data/sounds/close_door.mp3', + 'replace_tipracks': './data/sounds/replace_tipracks.mp3', + 'empty_trash': './data/sounds/empty_trash.mp3' + } +elif LANGUAGE_DICT[LANGUAGE] == 'esp': + VOICE_FILES_DICT = { + 'start': './data/sounds/started_process_esp.mp3', + 'finish': './data/sounds/finished_process_esp.mp3', + 'close_door': './data/sounds/close_door_esp.mp3', + 'replace_tipracks': './data/sounds/replace_tipracks_esp.mp3', + 'empty_trash': './data/sounds/empty_trash_esp.mp3' + } + +# Function definitions +def run_info(start,end,parameters = dict()): + info = {} + hostname = subprocess.run( + ['hostname'], + stdout=subprocess.PIPE, + stderr=subprocess.PIPE + ).stdout.decode('utf-8') + + info["RobotID"] = hostname + info["executedAction"] = ACTION + info["ProtocolID"] = PROTOCOL_ID + info["StartRunTime"] = start + info["FinishRunTime"] = end + info["parameters"] = parameters + # write json to file. This is going to be an api post. + #with open('run.json', 'w') as fp: + #json.dump(info, fp,indent=4) + +def check_door(): + return gpio.read_window_switches() + +def confirm_door_is_closed(): + if not robot.is_simulating(): + #Check if door is opened + if check_door() == False: + #Set light color to red and pause + gpio.set_button_light(1,0,0) + robot.pause() + voice_notification('close_door') + time.sleep(5) + confirm_door_is_closed() + else: + #Set light color to green + gpio.set_button_light(0,1,0) + +def start_run(): + voice_notification('start') + gpio.set_button_light(0,1,0) + now = datetime.now() + # dd/mm/YY H:M:S + start_time = now.strftime("%Y/%m/%d %H:%M:%S") + return start_time + +def finish_run(): + voice_notification('finish') + #Set light color to blue + gpio.set_button_light(0,0,1) + now = datetime.now() + # dd/mm/YY H:M:S + finish_time = now.strftime("%Y/%m/%d %H:%M:%S") + return finish_time + +def voice_notification(action): + if not robot.is_simulating(): + fname = VOICE_FILES_DICT[action] + if os.path.isfile(fname) is True: + subprocess.run( + ['mpg123', fname], + stdout=subprocess.PIPE, + stderr=subprocess.PIPE + ) + else: + robot.comment(f"Sound file does not exist. Call the technician") + +def reset_tipcount(file_path = '/data/B/tip_log.json'): + if os.path.isfile(file_path): + os.remove(file_path) + +def retrieve_tip_info(pip,tipracks,file_path = '/data/B/tip_log.json'): + global tip_log + if not tip_log['count'] or pip not in tip_log['count']: + tip_log['count'][pip] = 0 + if not robot.is_simulating(): + if os.path.isfile(file_path): + with open(file_path) as json_file: + data = json.load(json_file) + if 'P1000' in str(pip): + tip_log['count'][pip] = data['tips1000'] + elif 'P300' in str(pip): + tip_log['count'][pip] = data['tips300'] + elif 'P20' in str(pip): + tip_log['count'][pip] = data['tips20'] + + if "8-Channel" in str(pip): + tip_log['tips'][pip] = [tip for rack in tipracks for tip in rack.rows()[0]] + else: + tip_log['tips'][pip] = [tip for rack in tipracks for tip in rack.wells()] + + tip_log['max'][pip] = len(tip_log['tips'][pip]) + + return tip_log + +def save_tip_info(file_path = '/data/B/tip_log.json'): + data = {} + if not robot.is_simulating(): + if os.path.isfile(file_path): + os.rename(file_path,file_path + ".bak") + for pip in tip_log['count']: + if "P1000" in str(pip): + data['tips1000'] = tip_log['count'][pip] + elif "P300" in str(pip): + data['tips300'] = tip_log['count'][pip] + elif "P20" in str(pip): + data['tips20'] = tip_log['count'][pip] + + with open(file_path, 'a+') as outfile: + json.dump(data, outfile) + +def pick_up(pip,tiprack): + if tip_log['count'][pip] == tip_log['max'][pip]: + voice_notification('replace_tipracks') + robot.pause('Replace ' + str(pip.max_volume) + 'µl tipracks before \ +resuming.') + confirm_door_is_closed() + pip.reset_tipracks() + tip_log['count'][pip] = 0 + pip.pick_up_tip(tip_log['tips'][pip][tip_log['count'][pip]]) + tip_log['count'][pip] += 1 + +def drop(pip): + global switch + if "8-Channel" not in str(pip): + side = 1 if switch else -1 + drop_loc = robot.loaded_labwares[12].wells()[0].top().move(Point(x=side*20)) + pip.drop_tip(drop_loc,home_after=False) + switch = not switch + else: + drop_loc = robot.loaded_labwares[12].wells()[0].top().move(Point(x=20)) + pip.drop_tip(drop_loc,home_after=False) + +def mix_beads(reps, dests, pip, tiprack): + ## Dispense beads to deep well plate. + for i, m in enumerate(dests): + if not pip.hw_pipette['has_tip']: + pick_up(pip,tiprack) + dispense_default_speed = pip.flow_rate.dispense + pip.flow_rate.dispense = 600 + pip.mix(reps, 200, m.bottom(5)) + pip.flow_rate.dispense = dispense_default_speed + # PENDING TO FIX THIS blow_out + # pip.blow_out(m.top(-2)) + pip.aspirate(20, m.top(-2)) + drop(pip) + +def dispense_beads(reps,sources,dests,pip,tiprack): + ## Mix beads prior to dispensing. + pick_up(pip,tiprack) + for s in sources: + pip.mix(reps, 200, s.bottom(20)) + + ## Dispense beads to deep well plate. + for i, m in enumerate(dests): + if not pip.hw_pipette['has_tip']: + pick_up(pip,tiprack) + pip.transfer(200, dests[i//3], m.bottom(5), new_tip='never', air_gap=20) + pip.blow_out(m.top(-2)) + drop(pip) + pick_up(pip,tiprack) + pip.transfer(200, dests[i//3], m.bottom(5), new_tip='never', air_gap=20) + mix_beads(reps, dests, pip, tiprack) + +def remove_supernatant(sources,waste,pip,tiprack): + for i, m in enumerate(sources): + loc = m.bottom(1) + pick_up(pip,tiprack) + pip.transfer(800, loc, waste, air_gap=100, new_tip='never') + pip.blow_out(waste) + drop(pip) + +def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack,tipreuse): + for wash_set in wash_sets: + # transfer wash + pick_up(pip,tiprack) + for i, m in enumerate(dests): + magdeck.disengage() + wash_chan = wash_set[i//6] + pip.transfer( + 200, wash_chan.bottom(2), m.top(), new_tip='never', air_gap=20) + + # mix beads with wash + tips_loc = 0 + for i, m in enumerate(dests): + if tips_loc == 0: + if wash_set != 0: + drop(pip) + pip.pick_up_tip(tipreuse[0].rows()[0][tips_loc]) + else: + pip.pick_up_tip(tipreuse[0].rows()[0][tips_loc]) + + dispense_default_speed = pip.flow_rate.dispense + pip.flow_rate.dispense = 1500 + pip.mix(7, 200, m.bottom(2)) + pip.flow_rate.dispense = dispense_default_speed + pip.return_tip(home_after=False) + tips_loc += 1 + + magdeck.engage(height_from_base=MAGNET_HEIGHT) + # robot.delay(seconds=75, msg='Incubating on magnet for 75 seconds.') + + # remove supernatant + tips_loc = 0 + for i, m in enumerate(dests): + pip.pick_up_tip(tipreuse[0].rows()[0][tips_loc]) + aspire_default_speed = pip.flow_rate.aspirate + pip.flow_rate.aspirate = 75 + asp_loc = m.bottom(1.5) + pip.transfer(200, asp_loc, waste, new_tip='never', air_gap=20) + pip.flow_rate.aspirate = aspire_default_speed + pip.blow_out(waste) + pip.return_tip(home_after=False) + tips_loc += 1 + +def wash(wash_sets,dests,waste,magdeck,pip,tiprack): + for wash_set in wash_sets: + for i, m in enumerate(dests): + # transfer and mix wash with beads + magdeck.disengage() + wash_chan = wash_set[i//6] + pick_up(pip,tiprack) + pip.transfer( + 200, wash_chan.bottom(2), m.center(), new_tip='never', air_gap=20) + # Mix heigh has to be really close to bottom, it was 5 now reduced to 2, maybe should be 1? + dispense_default_speed = pip.flow_rate.dispense + pip.flow_rate.dispense = 1500 + pip.mix(7, 200, m.bottom(2)) + pip.flow_rate.dispense = dispense_default_speed + + magdeck.engage(height_from_base=MAGNET_HEIGHT) + # robot.delay(seconds=75, msg='Incubating on magnet for 75 seconds.') + + # remove supernatant + aspire_default_speed = pip.flow_rate.aspirate + pip.flow_rate.aspirate = 75 + asp_loc = m.bottom(1.5) + pip.transfer(200, asp_loc, waste, new_tip='never', air_gap=20) + pip.flow_rate.aspirate = aspire_default_speed + pip.blow_out(waste) + drop(pip) + +def elute_samples(sources,dests,buffer,magdeck,pip,tipracks): + ## dispense buffer + for i, m in enumerate(sources): + pick_up(pip,tipracks) + dispense_default_speed = pip.flow_rate.dispense + pip.flow_rate.dispense = 1500 + pip.transfer( + 50, buffer.bottom(2), m.bottom(1), new_tip='never', air_gap=10) + pip.mix(20, 200, m.bottom(1)) + pip.flow_rate.dispense = dispense_default_speed + drop(pip) + + ## Incubation steps + # robot.delay(minutes=5, msg='Incubating off magnet for 5 minutes.') + magdeck.engage(height_from_base=MAGNET_HEIGHT) + # robot.delay(seconds=120, msg='Incubating on magnet for 120 seconds.') + + aspire_default_speed = pip.flow_rate.aspirate + pip.flow_rate.aspirate = 50 + ## Dispense elutes in pcr plate. + for i, (m, e) in enumerate(zip(sources, dests)): + # tranfser and mix elution buffer with beads + asp_loc = m.bottom(1.5) + pick_up(pip,tipracks) + # transfer elution to new plate + pip.transfer(50, asp_loc, e, new_tip='never', air_gap=10) + pip.blow_out(e.top(-2)) + drop(pip) + pip.flow_rate.aspirate = aspire_default_speed + +def run(ctx: protocol_api.ProtocolContext): + global robot + robot = ctx + + # check if tipcount is being reset + if RESET_TIPCOUNT: + reset_tipcount() + + # confirm door is close + robot.comment(f"Please, close the door") + confirm_door_is_closed() + + # Begin run + start_time = start_run() + + # load labware and modules + ## ELUTION LABWARE + if ELUTION_LABWARE not in ELUTION_LW_DICT: + raise Exception('Invalid ELUTION_LABWARE. Must be one of the \ + following:\nopentrons aluminum biorad plate\nopentrons aluminum nest plate') + + elution_plate = robot.load_labware( + ELUTION_LW_DICT[ELUTION_LABWARE], '1', + 'elution plate') + + ## MAGNETIC PLATE LABWARE + magdeck = robot.load_module('magdeck', '10') + magdeck.disengage() + + if MAGPLATE_LABWARE not in MAGPLATE_LW_DICT: + raise Exception('Invalid MAGPLATE_LABWARE. Must be one of the \ +following:\nopentrons deep generic well plate\nnest deep generic well plate\nvwr deep generic well plate') + + magplate = magdeck.load_labware(MAGPLATE_LW_DICT[MAGPLATE_LABWARE]) + + ## WASTE LABWARE + if WASTE_LABWARE not in WASTE_LW_DICT: + raise Exception('Invalid WASTE_LABWARE. Must be one of the \ + following:\nnest 1 reservoir plate') + + waste = robot.load_labware( + WASTE_LW_DICT[WASTE_LABWARE], '11', 'waste reservoir').wells()[0].top(-10) + + ## REAGENT RESERVOIR + if REAGENT_LABWARE not in REAGENT_LW_DICT: + raise Exception('Invalid REAGENT_LABWARE. Must be one of the \ + following:\nnest 12 reservoir plate') + + reagent_res = robot.load_labware( + REAGENT_LW_DICT[REAGENT_LABWARE], '7', 'reagent reservoir') + + ## TIPS + # using standard tip definition despite actually using filter tips + # so that the tips can accommodate ~220µl per transfer for efficiency + tips300 = [ + robot.load_labware( + 'opentrons_96_tiprack_300ul', slot, '200µl filter tiprack') + for slot in ['2', '3', '4', '5', '6'] + ] + tipsreuse = [ + robot.load_labware( + 'opentrons_96_tiprack_300ul', slot, '200µl filter tiprack') + for slot in ['8'] + ] + tips1000 = [ + robot.load_labware('opentrons_96_filtertiprack_1000ul', slot, + '1000µl filter tiprack') + for slot in ['9'] + ] + + # reagents and samples + num_cols = math.ceil(NUM_SAMPLES/8) + mag_samples_m = magplate.rows()[0][:num_cols] + mag_samples_s = magplate.wells()[:NUM_SAMPLES] + elution_samples_m = elution_plate.rows()[0][:num_cols] + elution_buffer = reagent_res.wells()[0] + bead_buffer = reagent_res.wells()[1:5] + wash_sets = [reagent_res.wells()[i:i+2] for i in [5, 7, 9]] + + # pipettes + m300 = robot.load_instrument('p300_multi_gen2', 'left', tip_racks=tips300) + p1000 = robot.load_instrument('p1000_single_gen2', 'right', + tip_racks=tips1000) + + ## retrieve tip_log + retrieve_tip_info(p1000,tips1000) + retrieve_tip_info(m300,tips300) + + m300.flow_rate.aspirate = 150 + m300.flow_rate.dispense = 300 + m300.flow_rate.blow_out = 300 + p1000.flow_rate.aspirate = 100 + p1000.flow_rate.dispense = 1000 + p1000.flow_rate.blow_out = 1000 + + # start with magdeck off + magdeck.disengage() + + if(DISPENSE_BEADS): + # premix, transfer, and mix magnetic beads with sample + ## bead dests depending on number of samples + bead_dests = bead_buffer[:math.ceil(num_cols/4)] + dispense_beads(7,bead_dests,mag_samples_m,m300,tips300) + else: + # Mix bead + mix_beads(7, mag_samples_m,m300,tips300) + + # incubate off the magnet + # robot.delay(minutes=10, msg='Incubating off magnet for 10 minutes.') + + ## First incubate on magnet. + magdeck.engage(height_from_base=MAGNET_HEIGHT) + # robot.delay(minutes=7, msg='Incubating on magnet for 7 minutes.') + + # remove supernatant with P1000 + remove_supernatant(mag_samples_s,waste,p1000,tips1000) + + # empty trash + if NUM_SAMPLES > 24: + voice_notification('empty_trash') + robot.pause(f"Please, empty trash") + confirm_door_is_closed() + + # 3x washes + if REUSE_TIPS == True: + wash_reuse(wash_sets,mag_samples_m,waste,magdeck,m300,tips300,tipsreuse) + else: + wash(wash_sets,mag_samples_m,waste,magdeck,m300,tips300) + + # empty trash + if NUM_SAMPLES > 72: + voice_notification('empty_trash') + robot.pause(f"Please, empty trash") + confirm_door_is_closed() + + # elute samples + magdeck.disengage() + elute_samples(mag_samples_m,elution_samples_m,elution_buffer,magdeck,m300,tips300) + + # track final used tip + save_tip_info() + + magdeck.disengage() + + finish_time = finish_run() + + par = { + "NUM_SAMPLES" : NUM_SAMPLES, + "REAGENT_LABWARE" : REAGENT_LABWARE, + "MAGPLATE_LABWARE" : MAGPLATE_LABWARE, + "WASTE_LABWARE" : WASTE_LABWARE, + "ELUTION_LABWARE" : ELUTION_LABWARE, + "DISPENSE_BEADS" : DISPENSE_BEADS, + "LANGUAGE" : LANGUAGE, + "RESET_TIPCOUNT" : RESET_TIPCOUNT + } + + run_info(start_time, finish_time, par) From e561a418e9521400e23efcd8457be3cd63c116b8 Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Thu, 28 May 2020 12:19:56 +0200 Subject: [PATCH 77/95] Updated to last version --- ...tionB_protocol1_extraction_S3.ot2.apiv2.py | 44 +++++++++++++++++-- 1 file changed, 40 insertions(+), 4 deletions(-) diff --git a/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py b/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py index b1c6264..533e5d8 100644 --- a/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -5,6 +5,7 @@ import math import os import subprocess +import requests import json from datetime import datetime @@ -44,7 +45,7 @@ LANGUAGE = 'esp' RESET_TIPCOUNT = True PROTOCOL_ID = "0000-AA" - +URL = 'localhost' # End Parameters to adapt the protocol ## global vars @@ -137,6 +138,29 @@ } # Function definitions + +def write_to_error_log (info, reason): + date = datetime.now().strftime("%Y_%m_%d") + folder_date = os.path.join('/data', date) + time_now = datetime.now().strftime("%Y_%m_%d_%H_%M_%S") + json_file = time_now + '.json' + folder_file_name = os.path.join(folder_date, json_file) + folder_error_log = os.path.join(folder_date,'error.log') + if not os.path.exists(folder_date): + try: + os.makedirs(folder_date) + except: + return + try: + # Create a new file for dumping json data + with open (folder_file_name , 'w') as fh: + json.dump(info, fh, indent=4) + # Append status reason code to the log + with open(folder_error_log, 'a') as fh: + fh.write( time_now + ' Unable to accept the requests get error : '+ reason + '\n') + except: + return + def run_info(start,end,parameters = dict()): info = {} hostname = subprocess.run( @@ -151,9 +175,20 @@ def run_info(start,end,parameters = dict()): info["StartRunTime"] = start info["FinishRunTime"] = end info["parameters"] = parameters - # write json to file. This is going to be an api post. - #with open('run.json', 'w') as fp: - #json.dump(info, fp,indent=4) + + headers = {'Content-type': 'application/json'} + url_https = 'https://' + URL + url_http = 'http://' + URL + try: + r = requests.post(url_https, data=json.dumps(info), headers=headers) + except: + try: + r = requests.post(url_http, data=json.dumps(info), headers=headers) + except: + write_to_error_log(info, 'Server communication error') + return + if r.status_code > 201 : + write_to_error_log(info, str(r.status_code)) def check_door(): return gpio.read_window_switches() @@ -335,6 +370,7 @@ def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack,tipreuse): magdeck.engage(height_from_base=MAGNET_HEIGHT) # robot.delay(seconds=75, msg='Incubating on magnet for 75 seconds.') + robot.delay(seconds=15, msg='Incubating on magnet for 15 seconds.') # remove supernatant tips_loc = 0 From d64c4afa3f8ae5ce4231de71a40f92632243c51e Mon Sep 17 00:00:00 2001 From: saramonzon Date: Thu, 28 May 2020 12:29:04 +0200 Subject: [PATCH 78/95] changed protocol name in station C protocol 2 --- protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py index 864b223..7c31fb2 100644 --- a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py @@ -11,7 +11,7 @@ # Metadata metadata = { - 'protocolName': 'S3 Station C Protocol 1 pcr Version 1', + 'protocolName': 'S3 Station C Protocol 2 pcrmulti Version 1', 'author': 'Nick , Sara , Miguel ', 'source': 'Custom Protocol Request', 'apiLevel': '2.3' From c6183473a2a97359de866c6e45211c5fd0efb5d3 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Thu, 28 May 2020 12:41:27 +0200 Subject: [PATCH 79/95] changed action name for protocol 2 station c --- protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py index 7c31fb2..8ead6c7 100644 --- a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py @@ -31,7 +31,7 @@ PROTOCOL_ID = "0000-AA" # End Parameters to adapt the protocol -ACTION = "StationC-protocol1-pcr" +ACTION = "StationC-protocol2-pcrmulti" ## global vars ## initialize robot object From 245871c75637ece9054be6f9ad98b732b0296a1f Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Thu, 28 May 2020 14:03:42 +0200 Subject: [PATCH 80/95] Tip reuse is working --- ...tionB_protocol1_extraction_S3.ot2.apiv2.py | 59 ++++++++++++------- ...tionB_protocol1_extraction_S3.ot2.apiv2.py | 31 ++++++---- 2 files changed, 58 insertions(+), 32 deletions(-) diff --git a/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py b/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py index 533e5d8..6dcbe8a 100644 --- a/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -5,7 +5,7 @@ import math import os import subprocess -import requests +# mport requests import json from datetime import datetime @@ -179,14 +179,17 @@ def run_info(start,end,parameters = dict()): headers = {'Content-type': 'application/json'} url_https = 'https://' + URL url_http = 'http://' + URL - try: - r = requests.post(url_https, data=json.dumps(info), headers=headers) - except: - try: - r = requests.post(url_http, data=json.dumps(info), headers=headers) - except: - write_to_error_log(info, 'Server communication error') - return + # try: + # r = requests.post(url_https, data=json.dumps(info), headers=headers) + # except: + # try: + # r = requests.post(url_http, data=json.dumps(info), headers=headers) + # except: + # write_to_error_log(info, 'Server communication error') + # return + write_to_error_log(info, 'Server communication error') + return + if r.status_code > 201 : write_to_error_log(info, str(r.status_code)) @@ -342,6 +345,7 @@ def remove_supernatant(sources,waste,pip,tiprack): drop(pip) def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack,tipreuse): + wash_num = 0 for wash_set in wash_sets: # transfer wash pick_up(pip,tiprack) @@ -354,24 +358,31 @@ def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack,tipreuse): # mix beads with wash tips_loc = 0 for i, m in enumerate(dests): - if tips_loc == 0: - if wash_set != 0: + + if wash_num != 0: + if tips_loc == 0: drop(pip) - pip.pick_up_tip(tipreuse[0].rows()[0][tips_loc]) + pip.pick_up_tip(tipreuse[0].rows()[0][tips_loc]) else: - pip.pick_up_tip(tipreuse[0].rows()[0][tips_loc]) + if tips_loc != 0: + pick_up(pip,tiprack) dispense_default_speed = pip.flow_rate.dispense pip.flow_rate.dispense = 1500 pip.mix(7, 200, m.bottom(2)) pip.flow_rate.dispense = dispense_default_speed - pip.return_tip(home_after=False) + if wash_num != 0: + pip.return_tip(home_after=False) + else: + pip.drop_tip(tipreuse[0].rows()[0][tips_loc], home_after=False) tips_loc += 1 magdeck.engage(height_from_base=MAGNET_HEIGHT) # robot.delay(seconds=75, msg='Incubating on magnet for 75 seconds.') robot.delay(seconds=15, msg='Incubating on magnet for 15 seconds.') + wash_num += 1 + # remove supernatant tips_loc = 0 for i, m in enumerate(dests): @@ -382,9 +393,13 @@ def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack,tipreuse): pip.transfer(200, asp_loc, waste, new_tip='never', air_gap=20) pip.flow_rate.aspirate = aspire_default_speed pip.blow_out(waste) - pip.return_tip(home_after=False) + if wash_num != 2: + pip.return_tip(home_after=False) + else: + pip.drop_tip(home_after=False) tips_loc += 1 + def wash(wash_sets,dests,waste,magdeck,pip,tiprack): for wash_set in wash_sets: for i, m in enumerate(dests): @@ -491,7 +506,7 @@ def run(ctx: protocol_api.ProtocolContext): following:\nnest 12 reservoir plate') reagent_res = robot.load_labware( - REAGENT_LW_DICT[REAGENT_LABWARE], '7', 'reagent reservoir') + REAGENT_LW_DICT[REAGENT_LABWARE], '4', 'reagent reservoir') ## TIPS # using standard tip definition despite actually using filter tips @@ -499,17 +514,17 @@ def run(ctx: protocol_api.ProtocolContext): tips300 = [ robot.load_labware( 'opentrons_96_tiprack_300ul', slot, '200µl filter tiprack') - for slot in ['2', '3', '4', '5', '6'] + for slot in ['2', '3', '5', '6', '9'] ] tipsreuse = [ robot.load_labware( 'opentrons_96_tiprack_300ul', slot, '200µl filter tiprack') - for slot in ['8'] + for slot in ['7'] ] tips1000 = [ robot.load_labware('opentrons_96_filtertiprack_1000ul', slot, '1000µl filter tiprack') - for slot in ['9'] + for slot in ['8'] ] # reagents and samples @@ -545,9 +560,9 @@ def run(ctx: protocol_api.ProtocolContext): ## bead dests depending on number of samples bead_dests = bead_buffer[:math.ceil(num_cols/4)] dispense_beads(7,bead_dests,mag_samples_m,m300,tips300) - else: + # else: # Mix bead - mix_beads(7, mag_samples_m,m300,tips300) + # mix_beads(7, mag_samples_m,m300,tips300) # incubate off the magnet # robot.delay(minutes=10, msg='Incubating off magnet for 10 minutes.') @@ -557,7 +572,7 @@ def run(ctx: protocol_api.ProtocolContext): # robot.delay(minutes=7, msg='Incubating on magnet for 7 minutes.') # remove supernatant with P1000 - remove_supernatant(mag_samples_s,waste,p1000,tips1000) + # remove_supernatant(mag_samples_s,waste,p1000,tips1000) # empty trash if NUM_SAMPLES > 24: diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index bb6f8ab..51c0191 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -342,6 +342,7 @@ def remove_supernatant(sources,waste,pip,tiprack): drop(pip) def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack,tipreuse): + wash_num = 0 for wash_set in wash_sets: # transfer wash pick_up(pip,tiprack) @@ -354,23 +355,30 @@ def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack,tipreuse): # mix beads with wash tips_loc = 0 for i, m in enumerate(dests): - if tips_loc == 0: - if wash_set != 0: + + if wash_num != 0: + if tips_loc == 0: drop(pip) - pip.pick_up_tip(tipreuse[0].rows()[0][tips_loc]) + pip.pick_up_tip(tipreuse[0].rows()[0][tips_loc]) else: - pip.pick_up_tip(tipreuse[0].rows()[0][tips_loc]) + if tips_loc != 0: + pick_up(pip,tiprack) dispense_default_speed = pip.flow_rate.dispense pip.flow_rate.dispense = 1500 pip.mix(7, 200, m.bottom(2)) pip.flow_rate.dispense = dispense_default_speed - pip.return_tip(home_after=False) + if wash_num != 0: + pip.return_tip(home_after=False) + else: + pip.drop_tip(tipreuse[0].rows()[0][tips_loc], home_after=False) tips_loc += 1 magdeck.engage(height_from_base=MAGNET_HEIGHT) robot.delay(seconds=75, msg='Incubating on magnet for 75 seconds.') + wash_num += 1 + # remove supernatant tips_loc = 0 for i, m in enumerate(dests): @@ -381,7 +389,10 @@ def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack,tipreuse): pip.transfer(200, asp_loc, waste, new_tip='never', air_gap=20) pip.flow_rate.aspirate = aspire_default_speed pip.blow_out(waste) - pip.return_tip(home_after=False) + if wash_num != 2: + pip.return_tip(home_after=False) + else: + pip.drop_tip(home_after=False) tips_loc += 1 def wash(wash_sets,dests,waste,magdeck,pip,tiprack): @@ -490,7 +501,7 @@ def run(ctx: protocol_api.ProtocolContext): following:\nnest 12 reservoir plate') reagent_res = robot.load_labware( - REAGENT_LW_DICT[REAGENT_LABWARE], '7', 'reagent reservoir') + REAGENT_LW_DICT[REAGENT_LABWARE], '4', 'reagent reservoir') ## TIPS # using standard tip definition despite actually using filter tips @@ -498,17 +509,17 @@ def run(ctx: protocol_api.ProtocolContext): tips300 = [ robot.load_labware( 'opentrons_96_tiprack_300ul', slot, '200µl filter tiprack') - for slot in ['2', '3', '4', '5', '6'] + for slot in ['2', '3', '5', '6', '9'] ] tipsreuse = [ robot.load_labware( 'opentrons_96_tiprack_300ul', slot, '200µl filter tiprack') - for slot in ['8'] + for slot in ['7'] ] tips1000 = [ robot.load_labware('opentrons_96_filtertiprack_1000ul', slot, '1000µl filter tiprack') - for slot in ['9'] + for slot in ['8'] ] # reagents and samples From c4dbea5e5b4a7bc677c073919effa741cb7f090e Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Thu, 28 May 2020 14:07:15 +0200 Subject: [PATCH 81/95] Dropping tips after third wash --- .../stationB_protocol1_extraction_S3.ot2.apiv2.py | 2 +- protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py b/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py index 6dcbe8a..ffc4952 100644 --- a/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -393,7 +393,7 @@ def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack,tipreuse): pip.transfer(200, asp_loc, waste, new_tip='never', air_gap=20) pip.flow_rate.aspirate = aspire_default_speed pip.blow_out(waste) - if wash_num != 2: + if wash_num != 3: pip.return_tip(home_after=False) else: pip.drop_tip(home_after=False) diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index 51c0191..eff1db7 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -389,7 +389,7 @@ def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack,tipreuse): pip.transfer(200, asp_loc, waste, new_tip='never', air_gap=20) pip.flow_rate.aspirate = aspire_default_speed pip.blow_out(waste) - if wash_num != 2: + if wash_num != 3: pip.return_tip(home_after=False) else: pip.drop_tip(home_after=False) From 0af8792ff1633f73744c3e02ef724213a2d5561f Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Thu, 28 May 2020 14:09:29 +0200 Subject: [PATCH 82/95] Removed disengaing magdeck twice --- .../stationB_protocol1_extraction_S3.ot2.apiv2.py | 3 --- protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py | 3 --- 2 files changed, 6 deletions(-) diff --git a/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py b/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py index ffc4952..83e3f7b 100644 --- a/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -552,9 +552,6 @@ def run(ctx: protocol_api.ProtocolContext): p1000.flow_rate.dispense = 1000 p1000.flow_rate.blow_out = 1000 - # start with magdeck off - magdeck.disengage() - if(DISPENSE_BEADS): # premix, transfer, and mix magnetic beads with sample ## bead dests depending on number of samples diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index eff1db7..dce2088 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -547,9 +547,6 @@ def run(ctx: protocol_api.ProtocolContext): p1000.flow_rate.dispense = 1000 p1000.flow_rate.blow_out = 1000 - # start with magdeck off - magdeck.disengage() - if(DISPENSE_BEADS): # premix, transfer, and mix magnetic beads with sample ## bead dests depending on number of samples From 987583c8236dba37a4a1eb79b3b6abaeeb4870da Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Thu, 28 May 2020 14:09:37 +0200 Subject: [PATCH 83/95] Final version of experiment --- .../stationB_protocol1_extraction_S3.ot2.apiv2.py | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py b/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py index 83e3f7b..b7451e0 100644 --- a/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -557,9 +557,9 @@ def run(ctx: protocol_api.ProtocolContext): ## bead dests depending on number of samples bead_dests = bead_buffer[:math.ceil(num_cols/4)] dispense_beads(7,bead_dests,mag_samples_m,m300,tips300) - # else: + else: # Mix bead - # mix_beads(7, mag_samples_m,m300,tips300) + mix_beads(7, mag_samples_m,m300,tips300) # incubate off the magnet # robot.delay(minutes=10, msg='Incubating off magnet for 10 minutes.') @@ -568,7 +568,7 @@ def run(ctx: protocol_api.ProtocolContext): magdeck.engage(height_from_base=MAGNET_HEIGHT) # robot.delay(minutes=7, msg='Incubating on magnet for 7 minutes.') - # remove supernatant with P1000 + remove supernatant with P1000 # remove_supernatant(mag_samples_s,waste,p1000,tips1000) # empty trash From c9255b4b1d311c9774aafb20c2d0e8e508ac9511 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Thu, 28 May 2020 14:10:05 +0200 Subject: [PATCH 84/95] Added correct method for importing user python packages in the robot --- .../stationA_protocol1_buffer_S3.ot2.apiv2.py | 6 ++-- .../stationA_protocol2_beads_S3.ot2.apiv2.py | 5 ++- ...stationA_protocol3_lysates_S3.ot2.apiv2.py | 5 ++- ...tionB_protocol1_extraction_S3.ot2.apiv2.py | 34 ++++++++++++------- .../S3/stationC_protocol1_pcr_S3.ot2.apiv2.py | 5 ++- ...tationC_protocol2_pcrmulti_S3.ot2.apiv2.py | 6 +++- 6 files changed, 43 insertions(+), 18 deletions(-) diff --git a/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py index e6fe212..6881d58 100644 --- a/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py @@ -5,10 +5,12 @@ import math import os import subprocess -import requests import json from datetime import datetime - +custom_modules_path = "/var/user-packages/usr/lib/python3.7/site-packages" +if custom_modules_path not in sys.path: + sys.path.append(custom_modules_path) +import requests # Metadata metadata = { diff --git a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py index e2d9f94..e8f3830 100644 --- a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py @@ -5,9 +5,12 @@ import math import os import subprocess -import requests import json from datetime import datetime +custom_modules_path = "/var/user-packages/usr/lib/python3.7/site-packages" +if custom_modules_path not in sys.path: + sys.path.append(custom_modules_path) +import requests # metadata diff --git a/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py index 24edbce..6066c15 100644 --- a/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py @@ -5,9 +5,12 @@ import math import os import subprocess -import requests import json from datetime import datetime +custom_modules_path = "/var/user-packages/usr/lib/python3.7/site-packages" +if custom_modules_path not in sys.path: + sys.path.append(custom_modules_path) +import requests # metadata diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index bb6f8ab..c35e84b 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -5,10 +5,15 @@ import math import os import subprocess -import requests +import sys import json from datetime import datetime - +custom_modules_path = "/var/user-packages/usr/lib/python3.7/site-packages" +#sys.path.pop() +#sys.path.pop() +if custom_modules_path not in sys.path: + sys.path.append(custom_modules_path) +import requests # metadata metadata = { @@ -179,16 +184,18 @@ def run_info(start,end,parameters = dict()): headers = {'Content-type': 'application/json'} url_https = 'https://' + URL url_http = 'http://' + URL - try: - r = requests.post(url_https, data=json.dumps(info), headers=headers) - except: - try: - r = requests.post(url_http, data=json.dumps(info), headers=headers) - except: - write_to_error_log(info, 'Server communication error') - return - if r.status_code > 201 : - write_to_error_log(info, str(r.status_code)) + # try: + # r = requests.post(url_https, data=json.dumps(info), headers=headers) + # except: + # try: + # r = requests.post(url_http, data=json.dumps(info), headers=headers) + # except: + # write_to_error_log(info, 'Server communication error') + # return + #if r.status_code > 201 : + # write_to_error_log(info, str(r.status_code)) + write_to_error_log(info, 'Server communication error') + return def check_door(): return gpio.read_window_switches() @@ -445,6 +452,9 @@ def run(ctx: protocol_api.ProtocolContext): global robot robot = ctx + text = str(sys.path) + robot.comment(text) + # check if tipcount is being reset if RESET_TIPCOUNT: reset_tipcount() diff --git a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py index df16584..dd941b4 100644 --- a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py @@ -5,9 +5,12 @@ import math import os import subprocess -import requests import json from datetime import datetime +custom_modules_path = "/var/user-packages/usr/lib/python3.7/site-packages" +if custom_modules_path not in sys.path: + sys.path.append(custom_modules_path) +import requests # Metadata metadata = { diff --git a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py index 8ead6c7..d30e71a 100644 --- a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py @@ -5,9 +5,13 @@ import math import os import subprocess -import requests import json from datetime import datetime +custom_modules_path = "/var/user-packages/usr/lib/python3.7/site-packages" +if custom_modules_path not in sys.path: + sys.path.append(custom_modules_path) +import requests + # Metadata metadata = { From b4a8a49872b34376657cb38c388b8a2a911d073c Mon Sep 17 00:00:00 2001 From: saramonzon Date: Thu, 28 May 2020 14:11:16 +0200 Subject: [PATCH 85/95] removed unneded comments --- protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py | 2 -- 1 file changed, 2 deletions(-) diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index 186bc56..d0ed8e4 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -9,8 +9,6 @@ import json from datetime import datetime custom_modules_path = "/var/user-packages/usr/lib/python3.7/site-packages" -#sys.path.pop() -#sys.path.pop() if custom_modules_path not in sys.path: sys.path.append(custom_modules_path) import requests From 5a248a6f607b95534927072a87fcde0998ef183f Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Thu, 28 May 2020 14:14:05 +0200 Subject: [PATCH 86/95] Activated commented section, it works now --- ...tionB_protocol1_extraction_S3.ot2.apiv2.py | 37 ++++++++++--------- ...tionB_protocol1_extraction_S3.ot2.apiv2.py | 22 +++++------ 2 files changed, 29 insertions(+), 30 deletions(-) diff --git a/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py b/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py index b7451e0..202d0f6 100644 --- a/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/experiments/20200528-stationB_protocol1_extraction_release1.5/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -5,10 +5,13 @@ import math import os import subprocess -# mport requests +import sys import json from datetime import datetime - +custom_modules_path = "/var/user-packages/usr/lib/python3.7/site-packages" +if custom_modules_path not in sys.path: + sys.path.append(custom_modules_path) +import requests # metadata metadata = { @@ -179,19 +182,16 @@ def run_info(start,end,parameters = dict()): headers = {'Content-type': 'application/json'} url_https = 'https://' + URL url_http = 'http://' + URL - # try: - # r = requests.post(url_https, data=json.dumps(info), headers=headers) - # except: - # try: - # r = requests.post(url_http, data=json.dumps(info), headers=headers) - # except: - # write_to_error_log(info, 'Server communication error') - # return - write_to_error_log(info, 'Server communication error') - return - + try: + r = requests.post(url_https, data=json.dumps(info), headers=headers) + except: + try: + r = requests.post(url_http, data=json.dumps(info), headers=headers) + except: + write_to_error_log(info, 'Server communication error') + return if r.status_code > 201 : - write_to_error_log(info, str(r.status_code)) + write_to_error_log(info, str(r.status_code)) def check_door(): return gpio.read_window_switches() @@ -379,7 +379,6 @@ def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack,tipreuse): magdeck.engage(height_from_base=MAGNET_HEIGHT) # robot.delay(seconds=75, msg='Incubating on magnet for 75 seconds.') - robot.delay(seconds=15, msg='Incubating on magnet for 15 seconds.') wash_num += 1 @@ -399,7 +398,6 @@ def wash_reuse(wash_sets,dests,waste,magdeck,pip,tiprack,tipreuse): pip.drop_tip(home_after=False) tips_loc += 1 - def wash(wash_sets,dests,waste,magdeck,pip,tiprack): for wash_set in wash_sets: for i, m in enumerate(dests): @@ -461,6 +459,9 @@ def run(ctx: protocol_api.ProtocolContext): global robot robot = ctx + text = str(sys.path) + robot.comment(text) + # check if tipcount is being reset if RESET_TIPCOUNT: reset_tipcount() @@ -568,8 +569,8 @@ def run(ctx: protocol_api.ProtocolContext): magdeck.engage(height_from_base=MAGNET_HEIGHT) # robot.delay(minutes=7, msg='Incubating on magnet for 7 minutes.') - remove supernatant with P1000 - # remove_supernatant(mag_samples_s,waste,p1000,tips1000) + # remove supernatant with P1000 + remove_supernatant(mag_samples_s,waste,p1000,tips1000) # empty trash if NUM_SAMPLES > 24: diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index d0ed8e4..b5230a8 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -182,18 +182,16 @@ def run_info(start,end,parameters = dict()): headers = {'Content-type': 'application/json'} url_https = 'https://' + URL url_http = 'http://' + URL - # try: - # r = requests.post(url_https, data=json.dumps(info), headers=headers) - # except: - # try: - # r = requests.post(url_http, data=json.dumps(info), headers=headers) - # except: - # write_to_error_log(info, 'Server communication error') - # return - #if r.status_code > 201 : - # write_to_error_log(info, str(r.status_code)) - write_to_error_log(info, 'Server communication error') - return + try: + r = requests.post(url_https, data=json.dumps(info), headers=headers) + except: + try: + r = requests.post(url_http, data=json.dumps(info), headers=headers) + except: + write_to_error_log(info, 'Server communication error') + return + if r.status_code > 201 : + write_to_error_log(info, str(r.status_code)) def check_door(): return gpio.read_window_switches() From 004f31da3b4bb406871ed5e6f4ddd6f0588f4fd4 Mon Sep 17 00:00:00 2001 From: MiguelJulia Date: Thu, 28 May 2020 14:19:35 +0200 Subject: [PATCH 87/95] Removed print for debug --- protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py | 3 --- 1 file changed, 3 deletions(-) diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index b5230a8..b4913fc 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -459,9 +459,6 @@ def run(ctx: protocol_api.ProtocolContext): global robot robot = ctx - text = str(sys.path) - robot.comment(text) - # check if tipcount is being reset if RESET_TIPCOUNT: reset_tipcount() From fb6263214fe093551fefd8e973fb0dfffc383ef1 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Thu, 28 May 2020 14:27:07 +0200 Subject: [PATCH 88/95] added missing import sys to all protocols --- protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py | 1 + protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py | 1 + protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py | 1 + protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py | 2 +- protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py | 1 + protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py | 1 + 6 files changed, 6 insertions(+), 1 deletion(-) diff --git a/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py index 6881d58..978dfd3 100644 --- a/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py @@ -4,6 +4,7 @@ import time import math import os +import sys import subprocess import json from datetime import datetime diff --git a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py index e8f3830..aa18696 100644 --- a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py @@ -4,6 +4,7 @@ import time import math import os +import sys import subprocess import json from datetime import datetime diff --git a/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py index 6066c15..95d4136 100644 --- a/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py @@ -4,6 +4,7 @@ import time import math import os +import sys import subprocess import json from datetime import datetime diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index b4913fc..26692be 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -4,8 +4,8 @@ import time import math import os -import subprocess import sys +import subprocess import json from datetime import datetime custom_modules_path = "/var/user-packages/usr/lib/python3.7/site-packages" diff --git a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py index dd941b4..61e7fa3 100644 --- a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py @@ -4,6 +4,7 @@ import time import math import os +import sys import subprocess import json from datetime import datetime diff --git a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py index d30e71a..648a77d 100644 --- a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py @@ -4,6 +4,7 @@ import time import math import os +import sys import subprocess import json from datetime import datetime From fa1ec5a50981948706f48e79036d95a192638943 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Thu, 28 May 2020 15:55:27 +0200 Subject: [PATCH 89/95] logs in /data/logs instead of /data --- protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py | 2 +- protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py | 2 +- protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py | 2 +- protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py | 2 +- protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py | 2 +- protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py | 2 +- 6 files changed, 6 insertions(+), 6 deletions(-) diff --git a/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py index 978dfd3..c963191 100644 --- a/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol1_buffer_S3.ot2.apiv2.py @@ -105,7 +105,7 @@ def write_to_error_log (info, reason): date = datetime.now().strftime("%Y_%m_%d") - folder_date = os.path.join('/data', date) + folder_date = os.path.join('/data/logs', date) time_now = datetime.now().strftime("%Y_%m_%d_%H_%M_%S") json_file = time_now + '.json' folder_file_name = os.path.join(folder_date, json_file) diff --git a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py index aa18696..e65256f 100644 --- a/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol2_beads_S3.ot2.apiv2.py @@ -99,7 +99,7 @@ # Function definitions def write_to_error_log (info, reason): date = datetime.now().strftime("%Y_%m_%d") - folder_date = os.path.join('/data', date) + folder_date = os.path.join('/data/logs', date) time_now = datetime.now().strftime("%Y_%m_%d_%H_%M_%S") json_file = time_now + '.json' folder_file_name = os.path.join(folder_date, json_file) diff --git a/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py b/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py index 95d4136..78eccda 100644 --- a/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py +++ b/protocols/S3/stationA_protocol3_lysates_S3.ot2.apiv2.py @@ -108,7 +108,7 @@ def write_to_error_log (info, reason): date = datetime.now().strftime("%Y_%m_%d") - folder_date = os.path.join('/data', date) + folder_date = os.path.join('/data/logs', date) time_now = datetime.now().strftime("%Y_%m_%d_%H_%M_%S") json_file = time_now + '.json' folder_file_name = os.path.join(folder_date, json_file) diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index 26692be..ba24bc0 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -144,7 +144,7 @@ def write_to_error_log (info, reason): date = datetime.now().strftime("%Y_%m_%d") - folder_date = os.path.join('/data', date) + folder_date = os.path.join('/data/logs', date) time_now = datetime.now().strftime("%Y_%m_%d_%H_%M_%S") json_file = time_now + '.json' folder_file_name = os.path.join(folder_date, json_file) diff --git a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py index 61e7fa3..5b45438 100644 --- a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py @@ -173,7 +173,7 @@ def write_to_error_log (info, reason): date = datetime.now().strftime("%Y_%m_%d") - folder_date = os.path.join('/data', date) + folder_date = os.path.join('/data/logs', date) time_now = datetime.now().strftime("%Y_%m_%d_%H_%M_%S") json_file = time_now + '.json' folder_file_name = os.path.join(folder_date, json_file) diff --git a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py index 648a77d..7114762 100644 --- a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py @@ -137,7 +137,7 @@ def write_to_error_log (info, reason): date = datetime.now().strftime("%Y_%m_%d") - folder_date = os.path.join('/data', date) + folder_date = os.path.join('/data/logs', date) time_now = datetime.now().strftime("%Y_%m_%d_%H_%M_%S") json_file = time_now + '.json' folder_file_name = os.path.join(folder_date, json_file) From fbfa3979164340236dca2ea4d36d282013aa9b68 Mon Sep 17 00:00:00 2001 From: luissian Date: Thu, 28 May 2020 17:27:36 +0200 Subject: [PATCH 90/95] fixing bug with wrong indent in sending rest api information --- .../S3/stationC_protocol1_pcr_S3.ot2.apiv2.py | 22 +++++++++---------- 1 file changed, 11 insertions(+), 11 deletions(-) diff --git a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py index df16584..bc5a612 100644 --- a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py @@ -205,19 +205,19 @@ def run_info(start,end,parameters = dict()): info["FinishRunTime"] = end info["parameters"] = parameters - headers = {'Content-type': 'application/json'} - url_https = 'https://' + URL - url_http = 'http://' + URL + headers = {'Content-type': 'application/json'} + url_https = 'https://' + URL + url_http = 'http://' + URL + try: + r = requests.post(url_https, data=json.dumps(info), headers=headers) + except: try: - r = requests.post(url_https, data=json.dumps(info), headers=headers) + r = requests.post(url_http, data=json.dumps(info), headers=headers) except: - try: - r = requests.post(url_http, data=json.dumps(info), headers=headers) - except: - write_to_error_log(info, 'Server communication error') - return - if r.status_code > 201 : - write_to_error_log(info, str(r.status_code)) + write_to_error_log(info, 'Server communication error') + return + if r.status_code > 201 : + write_to_error_log(info, str(r.status_code)) def check_door(): From 3855882d9ab74be766b07a24d84c9d91c0e55fc1 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Fri, 29 May 2020 11:49:33 +0200 Subject: [PATCH 91/95] fixed URL variable missing in protocol 2 station c --- protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py | 1 + 1 file changed, 1 insertion(+) diff --git a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py index 7114762..7450d09 100644 --- a/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol2_pcrmulti_S3.ot2.apiv2.py @@ -34,6 +34,7 @@ LANGUAGE = 'esp' RESET_TIPCOUNT = False PROTOCOL_ID = "0000-AA" +URL = "localhost" # End Parameters to adapt the protocol ACTION = "StationC-protocol2-pcrmulti" From 30b478780bd2484e0113e6409650c85ae7f6a1a5 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Fri, 29 May 2020 14:43:06 +0200 Subject: [PATCH 92/95] Fixed comment before pause in protocol 1 station c --- protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py index 5b45438..4652fd0 100644 --- a/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py +++ b/protocols/S3/stationC_protocol1_pcr_S3.ot2.apiv2.py @@ -590,8 +590,9 @@ def run(ctx: protocol_api.ProtocolContext): if TRANSFER_MASTERMIX: transfer_mastermix(mm_tube, dests, p300, p20, tips300, tips20) if TRANSFER_SAMPLES: + robot.comment(f"Please, check that all wells have received the right ammount of mastermix") if not robot.is_simulating(): - robot.pause(f"Please, check that all wells have received the right ammount of mastermix") + robot.pause() # transfer samples to corresponding locations if TRANSFER_SAMPLES: From ac8b5e8eecbc34111db163d750c61fef86943777 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Fri, 29 May 2020 14:57:04 +0200 Subject: [PATCH 93/95] Changed remove trash in protocol B exactly as asked. --- .../stationB_protocol1_extraction_S3.ot2.apiv2.py | 14 +++++++------- 1 file changed, 7 insertions(+), 7 deletions(-) diff --git a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py index ba24bc0..ccf2467 100644 --- a/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py +++ b/protocols/S3/stationB_protocol1_extraction_S3.ot2.apiv2.py @@ -566,11 +566,17 @@ def run(ctx: protocol_api.ProtocolContext): magdeck.engage(height_from_base=MAGNET_HEIGHT) robot.delay(minutes=7, msg='Incubating on magnet for 7 minutes.') + # empty trash + if NUM_SAMPLES > 48: + voice_notification('empty_trash') + robot.pause(f"Please, empty trash") + confirm_door_is_closed() + # remove supernatant with P1000 remove_supernatant(mag_samples_s,waste,p1000,tips1000) # empty trash - if NUM_SAMPLES > 24: + if NUM_SAMPLES > 48: voice_notification('empty_trash') robot.pause(f"Please, empty trash") confirm_door_is_closed() @@ -581,12 +587,6 @@ def run(ctx: protocol_api.ProtocolContext): else: wash(wash_sets,mag_samples_m,waste,magdeck,m300,tips300) - # empty trash - if NUM_SAMPLES > 72: - voice_notification('empty_trash') - robot.pause(f"Please, empty trash") - confirm_door_is_closed() - # elute samples magdeck.disengage() elute_samples(mag_samples_m,elution_samples_m,elution_buffer,magdeck,m300,tips300) From d7bbc86692d5467aeca3b2dc85b56ec2d6d16df6 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Fri, 29 May 2020 15:09:08 +0200 Subject: [PATCH 94/95] added changelog changes for new release --- CHANGELOG | 31 +++++++++++++++++++++++++++++++ 1 file changed, 31 insertions(+) diff --git a/CHANGELOG b/CHANGELOG index 96783aa..fb288ae 100644 --- a/CHANGELOG +++ b/CHANGELOG @@ -1,3 +1,34 @@ +## Version 1.7 + +New features: +- Added json sending using request. Requests python module is installed in all robots. +- Automatic tests added using github actions for all protocols (except C station due to a bug in opentrons_simulate v.3.17.0 ) +- New protocol for using multi p20 pipette in station C for transferring extracts. +- Now there are two pauses in B station for removing trash tips when > 48 samples. One after mix y another after remove_supernatant. +- Added tip-reuse option for Station B protocol 1 (extraction). +- Added labware definition for biotix tip rack 300 ul (needs testing). +- Added labware definition for biotix tip rack 1000 ul (needs testing). +- Added labware ecogen deepwell to protocols A (this was missing in last release). + +Protocol modification: +- For MM3 distribution in station C protocol 1 tip is changed every three columns. +- Once mastermix is mixed in station C protocol 1 tip is changed. +- Only one p20 rack tips in station C protocol 1 in slot 6. + +Bug fixes: +- Fixed unset variable in dispense_beads protocol 1 station B. 2ab984b +- + +## Version 1.6 +Hotfix: +- Fix bug in hommogenize function in C protocol, used pip instead of +p300. + +## Version 1.5 +Hotfix: +- Removed comments where MAGNET_HEIGHT variable that were bothering +opentronsweb + ## Version 1.4 Documentation: - Added user manual in spanish and english. From a4cbfdf37ee670a66c8fd3ca521e8d3c4418ce70 Mon Sep 17 00:00:00 2001 From: saramonzon Date: Fri, 29 May 2020 15:10:32 +0200 Subject: [PATCH 95/95] changed changelog with new modifications --- CHANGELOG | 1 - 1 file changed, 1 deletion(-) diff --git a/CHANGELOG b/CHANGELOG index fb288ae..f652a83 100644 --- a/CHANGELOG +++ b/CHANGELOG @@ -17,7 +17,6 @@ Protocol modification: Bug fixes: - Fixed unset variable in dispense_beads protocol 1 station B. 2ab984b -- ## Version 1.6 Hotfix:

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