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[documentation] Give correct arguments to read simulators #53

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yoann-dufresne opened this issue Apr 26, 2019 · 3 comments
Open

[documentation] Give correct arguments to read simulators #53

yoann-dufresne opened this issue Apr 26, 2019 · 3 comments
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@yoann-dufresne
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I'm trying to use CAMISIM to generate a big fastq file containing community reads with no error.

If I understand well, this is possible using wgsim read simulator with the option "errorfree" (I saw that in the code).
So, I changed the config file with theses values :
readsim=tools/wgsim/wgsim
error_profiles=errorfree

But this is not that simple.
CAMISIM wants a file as input for the error_profiles value.
How can I change the values for a working pipeline ?

I think that it should be nice to have one or more wiki pages to explain the possible values in the config file.

@AlphaSquad
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AlphaSquad commented Apr 26, 2019

You are right, there should be a description for every read simulator how to modify the config accordingly, and the way to do this for the wgsim simulator is particularly ugly. I will need to have a look at that.

Having said that, the way it should work right now (changing the following 4 options):
readsim=tools/wgsim/wgsim
error_profiles=.
# CAMISIM expects a directory, but the option is not really required
profile=0.0
# error-rate
type=wgsim

@AlphaSquad AlphaSquad added this to the v2.0 milestone Aug 25, 2021
@yazhinia
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yazhinia commented Feb 11, 2022

How can we do the error-free or customized error rate with the art_illumina-2.3.6/art_illumina read simulator?

@AlphaSquad
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Error-free can only be done with wgsim (see my comment above for this). It should not make a difference whether you use ART or wgsim here.
For using the custom error profiles of ART please have a look at this pull request which describes how to add and use you own trained error profile: #88

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