Open
Description
After adding a chamber, the design does not compile because it cannot find proper source/sink terminals from it. Here is the code and error I am getting:
Code:
IMPORT CHAMBER
DEVICE covid_test_circuit
LAYER FLOW
# pm -> preamplification master mix (input)
# op -> oligo P solution (input)
# dw -> ultrapure DEPC water (input)
# dm -> detection master mix (input)
# od -> oligo D solution (input)
# ps -> pretreated sample (input)
# wp -> waste product post melting analysis (output)
PORT pm, op, dw, dm, od, ps, wp portRadius=700;
CHAMBER ch_melt_analysis width=9000 height=250 length=5000;
# Connects different channels together
NODE n1_reagmix, n2_sampmix, n3_reagmix, n4_sampmix, n5_dwsplit, n6_diluter;
# Pumps to control flow rate of DEPC water to 2nd set of reagents and diluter
PUMP pu1_dw_dil, pu2_dw_reag, pu3_pcr1, pu4_pcr2 length=300 width=600 flowChannelWidth=800;
V MIXER preamp_reagmixer;
H MIXER diluter;
V MIXER nested_reagmixer;
# Length/bends assume flow rate of ~ 8.042 uL/min for PCR
V CURVED MIXER pcr1_revt bendLength=8000 numberOfBends=5;
V CURVED MIXER pcr1_stpdn bendLength=8000 numberOfBends=5;
V CURVED MIXER pcr1_denat bendLength=10000 numberOfBends=6;
V CURVED MIXER pcr1_cyc1 bendLength=4500 numberOfBends=20;
V CURVED MIXER pcr1_cyc2 bendLength=30000 numberOfBends=11;
V CURVED MIXER pcr1_finext bendLength=5000 numberOfBends= 4;
V CURVED MIXER pcr2_denat bendLength=10000 numberOfBends=6;
V CURVED MIXER pcr2_cyc1 bendLength=12500 numberOfBends=18;
# Should be incubator/chamber, but could not get this component to work, so
# a curved mixer was used instead
# H CURVED MIXER melt_analysis bendLength=1000 numberOfBends=10;
### PREAMPLIFICATION STEP
## REAGENT PREP
# Split Ultrapure DEPC water via a node
# for use in different parts of the circuit
CHANNEL c0_dw from dw to n5_dwsplit channelWidth=800;
# Join reagents via a node
CHANNEL c1_pm from pm to n1_reagmix channelWidth=800;
CHANNEL c2_op from op to n1_reagmix channelWidth=800;
CHANNEL c3_dw from n5_dwsplit to n1_reagmix channelWidth=800;
# Connect sample to separate node
CHANNEL c4_ps from ps to n2_sampmix channelWidth=800;
# Connect reagents node with sample node
CHANNEL c5_combine from n1_reagmix to n2_sampmix channelWidth=800;
# Send combined products to mixer
CHANNEL c6_mix from n2_sampmix to preamp_reagmixer channelWidth=800;
# Pump combined products to target flow rate of 8.042 uL/min
CHANNEL c7_pump from preamp_reagmixer 2 to pu3_pcr1 1 channelWidth=800;
## PERFORM ROUND 1 PCR
# Reverse transcription step
CHANNEL c8_revt from pu3_pcr1 2 to pcr1_revt 2 channelWidth=800;
# Temperature step down step
CHANNEL c9_stpdn from pcr1_revt to pcr1_stpdn channelWidth=800;
# Initial denaturation step
CHANNEL c10_denat from pcr1_stpdn to pcr1_denat channelWidth=800;
# 1st temperature cycle (6 cycles)
CHANNEL c11_cyc1 from pcr1_denat to pcr1_cyc1 channelWidth=800;
# 2nd temperature cycle (22 cycles)
CHANNEL c12_cyc2 from pcr1_cyc1 to pcr1_cyc2 channelWidth=800;
# Final extension
CHANNEL c13_fin from pcr1_cyc2 to pcr1_finext channelWidth=800;
### NESTED AMPLIFICATION STEP
## REAGENT PREP
# Amplified sample dilution
CHANNEL c14_dil from pcr1_finext to n6_diluter channelWidth=800;
# Pump water from DEPC connect to ensure proper flowrate for dilution
CHANNEL c15_pump from n5_dwsplit to pu1_dw_dil 1 channelWidth=800;
CHANNEL c16_dw from pu1_dw_dil 2 to n6_diluter 1 channelWidth=800;
CHANNEL c17_mix from n6_diluter to diluter channelWidth=800;
# Join reagents via node
CHANNEL c18_dm from dm to n3_reagmix channelWidth=800;
CHANNEL c19_od from od to n3_reagmix channelWidth=800;
# Pump DEPC water to control flowrate for proper mixing ratios
CHANNEL c20_pump from n5_dwsplit 1 to pu2_dw_reag 1 channelWidth=800;
CHANNEL c21_dw from pu2_dw_reag 2 to n3_reagmix 1 channelWidth=800;
# Connect diluted preamp sample to different node
CHANNEL c22_dsamp from diluter to n4_sampmix channelWidth=800;
# Connect reagents node to sample node
CHANNEL c23_combine from n3_reagmix to n4_sampmix channelWidth=800;
# Send combined products to mixer
CHANNEL c24_mix from n4_sampmix to nested_reagmixer channelWidth=800;
# Pump combined products to target flow rate of 8.042 uL/min
CHANNEL c25_pump from nested_reagmixer 2 to pu4_pcr2 1 channelWidth=800;
## PERFORM ROUND 2 PCR
# Inital denaturation step
CHANNEL c26_denat from pu4_pcr2 2 to pcr2_denat 2 channelWidth=800;
# Temperature cycling (45 cycles)
CHANNEL c27_cyc1 from pcr2_denat to pcr2_cyc1 channelWidth=800;
### PERFORM MELTING ANALYSIS
# Move fully amplified sample to melting analysis chamber
CHANNEL c28_analyte from pcr2_cyc1 to ch_melt_analysis 1 channelWidth=800;
# Move analyzed sample to waste product port
CHANNEL c29_done from ch_melt_analysis 2 to wp channelWidth=800;
END LAYER
# LAYER CONTROL
# # Control port for valve
# PORT melt_valve_port portRadius=700;
# # Valves to control fluide flow in/out of melting analysis chamber
# VALVE melt_valve1 on c28_analyte;
# VALVE melt_valve2 on c29_done;
# # Net to connect valve input to open/close both simultaneously
# NET valve_net from melt_valve_port 1 to melt_valve1 4, melt_valve2 4 channelWidth=50;
# END LAYER
Output:
_____ _ _ _
| ___| |_ _(_) __ _(_)
| |_ | | | | | |/ _` | |
| _| | | |_| | | (_| | |
|_| |_|\__,_|_|\__, |_|
|___/
output dir: /var/www/fluigicad.org/jobs/tmp__6083867ec4667500180b7ad7/output
Running File: /var/www/fluigicad.org/jobs/tmp__6083867ec4667500180b7ad7/ctc2.uf
Creating the output directory:
Computing Constraints
Pulling Default Values of Components
Could not retrieve default parameters for CHAMBER
Expecting value: line 1 column 1 (char 0)
Warning: Could not pull default values for ch_melt_analysis of type :CHAMBER
Pulling Dimensions of Components
Could not retrieve dimensions for CHAMBER
Expecting value: line 1 column 1 (char 0)
Warning: Could not pull default values for ch_melt_analysis of type :CHAMBER
Pulling Terminals of Components
Could not retrieve dimensions for CHAMBER
Expecting value: line 1 column 1 (char 0)
Warning: Could not pull terminal data for ch_melt_analysis of type: CHAMBER
Setting Default MAX Dimensions to the device: (76200, 76200)
Starting terminal/port assignment ...
No port assigned to connection ref - c0_dw:dw:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c0_dw:n5_dwsplit:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c1_pm:pm:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c1_pm:n1_reagmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c2_op:op:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c2_op:n1_reagmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c3_dw:n5_dwsplit:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c3_dw:n1_reagmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c4_ps:ps:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c4_ps:n2_sampmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c5_combine:n1_reagmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c5_combine:n2_sampmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c6_mix:n2_sampmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c6_mix:preamp_reagmixer:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c9_stpdn:pcr1_revt:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c9_stpdn:pcr1_stpdn:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c10_denat:pcr1_stpdn:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c10_denat:pcr1_denat:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c11_cyc1:pcr1_denat:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c11_cyc1:pcr1_cyc1:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c12_cyc2:pcr1_cyc1:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c12_cyc2:pcr1_cyc2:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c13_fin:pcr1_cyc2:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c13_fin:pcr1_finext:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c14_dil:pcr1_finext:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c14_dil:n6_diluter:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c15_pump:n5_dwsplit:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c17_mix:n6_diluter:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c17_mix:diluter:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c18_dm:dm:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c18_dm:n3_reagmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c19_od:od:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c19_od:n3_reagmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c22_dsamp:diluter:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c22_dsamp:n4_sampmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c23_combine:n3_reagmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c23_combine:n4_sampmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c24_mix:n4_sampmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c24_mix:nested_reagmixer:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c27_cyc1:pcr2_denat:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c27_cyc1:pcr2_cyc1:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c28_analyte:pcr2_cyc1:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c29_done:wp:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
output: /var/www/fluigicad.org/jobs/tmp__6083867ec4667500180b7ad7/output/covid_test_circuit.dot
Parsing Layers...
Layer 0:
Name - "FLOW_0"
ID - "0"
Type - "FLOW"
Group - "0"
Component - pm
xpos - 0
ypos - 0
x-span - 1400
y-span - 1400
spacing - 1000
Port - 1
Port.x - 700
Port.y - 700
Adding to FLOW ...
Component - op
xpos - 0
ypos - 0
x-span - 1400
y-span - 1400
spacing - 1000
Port - 1
Port.x - 700
Port.y - 700
Adding to FLOW ...
Component - dw
xpos - 0
ypos - 0
x-span - 1400
y-span - 1400
spacing - 1000
Port - 1
Port.x - 700
Port.y - 700
Adding to FLOW ...
Component - dm
xpos - 0
ypos - 0
x-span - 1400
y-span - 1400
spacing - 1000
Port - 1
Port.x - 700
Port.y - 700
Adding to FLOW ...
Component - od
xpos - 0
ypos - 0
x-span - 1400
y-span - 1400
spacing - 1000
Port - 1
Port.x - 700
Port.y - 700
Adding to FLOW ...
Component - ps
xpos - 0
ypos - 0
x-span - 1400
y-span - 1400
spacing - 1000
Port - 1
Port.x - 700
Port.y - 700
Adding to FLOW ...
Component - wp
xpos - 0
ypos - 0
x-span - 1400
y-span - 1400
spacing - 1000
Port - 1
Port.x - 700
Port.y - 700
Adding to FLOW ...
Component - ch_melt_analysis
xpos - 0
ypos - 0
x-span - -1
y-span - -1
spacing - 9000
Adding to FLOW ...
Component - n1_reagmix
xpos - 0
ypos - 0
x-span - 800
y-span - 800
spacing - 1000
Port - 1
Port.x - 0
Port.y - 0
Adding to FLOW ...
Component - n2_sampmix
xpos - 0
ypos - 0
x-span - 800
y-span - 800
spacing - 1000
Port - 1
Port.x - 0
Port.y - 0
Adding to FLOW ...
Component - n3_reagmix
xpos - 0
ypos - 0
x-span - 800
y-span - 800
spacing - 1000
Port - 1
Port.x - 0
Port.y - 0
Adding to FLOW ...
Component - n4_sampmix
xpos - 0
ypos - 0
x-span - 800
y-span - 800
spacing - 1000
Port - 1
Port.x - 0
Port.y - 0
Adding to FLOW ...
Component - n5_dwsplit
xpos - 0
ypos - 0
x-span - 800
y-span - 800
spacing - 1000
Port - 1
Port.x - 0
Port.y - 0
Adding to FLOW ...
Component - n6_diluter
xpos - 0
ypos - 0
x-span - 800
y-span - 800
spacing - 1000
Port - 1
Port.x - 0
Port.y - 0
Adding to FLOW ...
Component - pu1_dw_dil
xpos - 0
ypos - 0
x-span - 800
y-span - 2300
spacing - 1000
Port - 1
Port.x - 400
Port.y - 0
Port - 2
Port.x - 400
Port.y - 2300
Port - 3
Port.x - 400
Port.y - 150
Port - 4
Port.x - 400
Port.y - 1150
Port - 5
Port.x - 400
Port.y - 2150
Adding to FLOW ...
Component - pu2_dw_reag
xpos - 0
ypos - 0
x-span - 800
y-span - 2300
spacing - 1000
Port - 1
Port.x - 400
Port.y - 0
Port - 2
Port.x - 400
Port.y - 2300
Port - 3
Port.x - 400
Port.y - 150
Port - 4
Port.x - 400
Port.y - 1150
Port - 5
Port.x - 400
Port.y - 2150
Adding to FLOW ...
Component - pu3_pcr1
xpos - 0
ypos - 0
x-span - 800
y-span - 2300
spacing - 1000
Port - 1
Port.x - 400
Port.y - 0
Port - 2
Port.x - 400
Port.y - 2300
Port - 3
Port.x - 400
Port.y - 150
Port - 4
Port.x - 400
Port.y - 1150
Port - 5
Port.x - 400
Port.y - 2150
Adding to FLOW ...
Component - pu4_pcr2
xpos - 0
ypos - 0
x-span - 800
y-span - 2300
spacing - 1000
Port - 1
Port.x - 400
Port.y - 0
Port - 2
Port.x - 400
Port.y - 2300
Port - 3
Port.x - 400
Port.y - 150
Port - 4
Port.x - 400
Port.y - 1150
Port - 5
Port.x - 400
Port.y - 2150
Adding to FLOW ...
Component - preamp_reagmixer
xpos - 0
ypos - 0
x-span - 4060
y-span - 4860
spacing - 1000
Port - 1
Port.x - 2030
Port.y - 0
Port - 2
Port.x - 2030
Port.y - 4860
Adding to FLOW ...
Component - diluter
xpos - 0
ypos - 0
x-span - 4060
y-span - 4860
spacing - 1000
Port - 1
Port.x - 2030
Port.y - 0
Port - 2
Port.x - 2030
Port.y - 4860
Adding to FLOW ...
Component - nested_reagmixer
xpos - 0
ypos - 0
x-span - 4060
y-span - 4860
spacing - 1000
Port - 1
Port.x - 2030
Port.y - 0
Port - 2
Port.x - 2030
Port.y - 4860
Adding to FLOW ...
Component - pcr1_revt
xpos - 0
ypos - 0
x-span - 10830
y-span - 21100
spacing - 1000
Port - 1
Port.x - 5415
Port.y - 0
Port - 2
Port.x - 5415
Port.y - 21100
Adding to FLOW ...
Component - pcr1_stpdn
xpos - 0
ypos - 0
x-span - 10830
y-span - 21100
spacing - 1000
Port - 1
Port.x - 5415
Port.y - 0
Port - 2
Port.x - 5415
Port.y - 21100
Adding to FLOW ...
Component - pcr1_denat
xpos - 0
ypos - 0
x-span - 12830
y-span - 25160
spacing - 1000
Port - 1
Port.x - 6415
Port.y - 0
Port - 2
Port.x - 6415
Port.y - 25160
Adding to FLOW ...
Component - pcr1_cyc1
xpos - 0
ypos - 0
x-span - 7330
y-span - 82000
spacing - 1000
Port - 1
Port.x - 3665
Port.y - 0
Port - 2
Port.x - 3665
Port.y - 82000
Adding to FLOW ...
Component - pcr1_cyc2
xpos - 0
ypos - 0
x-span - 32830
y-span - 45460
spacing - 1000
Port - 1
Port.x - 16415
Port.y - 0
Port - 2
Port.x - 16415
Port.y - 45460
Adding to FLOW ...
Component - pcr1_finext
xpos - 0
ypos - 0
x-span - 7830
y-span - 17040
spacing - 1000
Port - 1
Port.x - 3915
Port.y - 0
Port - 2
Port.x - 3915
Port.y - 17040
Adding to FLOW ...
Component - pcr2_denat
xpos - 0
ypos - 0
x-span - 12830
y-span - 25160
spacing - 1000
Port - 1
Port.x - 6415
Port.y - 0
Port - 2
Port.x - 6415
Port.y - 25160
Adding to FLOW ...
Component - pcr2_cyc1
xpos - 0
ypos - 0
x-span - 15330
y-span - 73880
spacing - 1000
Port - 1
Port.x - 7665
Port.y - 0
Port - 2
Port.x - 7665
Port.y - 73880
Adding to FLOW ...
Connection - c0_dw
Source - dw
Source Terminal - 1
Sink - n5_dwsplit
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c1_pm
Source - pm
Source Terminal - 1
Sink - n1_reagmix
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c2_op
Source - op
Source Terminal - 1
Sink - n1_reagmix
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c3_dw
Source - n5_dwsplit
Source Terminal - 1
Sink - n1_reagmix
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c4_ps
Source - ps
Source Terminal - 1
Sink - n2_sampmix
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c5_combine
Source - n1_reagmix
Source Terminal - 1
Sink - n2_sampmix
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c6_mix
Source - n2_sampmix
Source Terminal - 1
Sink - preamp_reagmixer
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c7_pump
Source - preamp_reagmixer
Source Terminal - 2
Sink - pu3_pcr1
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c8_revt
Source - pu3_pcr1
Source Terminal - 2
Sink - pcr1_revt
Sink Terminal - 2
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c9_stpdn
Source - pcr1_revt
Source Terminal - 1
Sink - pcr1_stpdn
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c10_denat
Source - pcr1_stpdn
Source Terminal - 1
Sink - pcr1_denat
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c11_cyc1
Source - pcr1_denat
Source Terminal - 1
Sink - pcr1_cyc1
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c12_cyc2
Source - pcr1_cyc1
Source Terminal - 1
Sink - pcr1_cyc2
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c13_fin
Source - pcr1_cyc2
Source Terminal - 1
Sink - pcr1_finext
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c14_dil
Source - pcr1_finext
Source Terminal - 1
Sink - n6_diluter
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c15_pump
Source - n5_dwsplit
Source Terminal - 1
Sink - pu1_dw_dil
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c16_dw
Source - pu1_dw_dil
Source Terminal - 2
Sink - n6_diluter
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c17_mix
Source - n6_diluter
Source Terminal - 1
Sink - diluter
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c18_dm
Source - dm
Source Terminal - 1
Sink - n3_reagmix
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c19_od
Source - od
Source Terminal - 1
Sink - n3_reagmix
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c20_pump
Source - n5_dwsplit
Source Terminal - 1
Sink - pu2_dw_reag
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c21_dw
Source - pu2_dw_reag
Source Terminal - 2
Sink - n3_reagmix
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c22_dsamp
Source - diluter
Source Terminal - 1
Sink - n4_sampmix
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c23_combine
Source - n3_reagmix
Source Terminal - 1
Sink - n4_sampmix
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c24_mix
Source - n4_sampmix
Source Terminal - 1
Sink - nested_reagmixer
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c25_pump
Source - nested_reagmixer
Source Terminal - 2
Sink - pu4_pcr2
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c26_denat
Source - pu4_pcr2
Source Terminal - 2
Sink - pcr2_denat
Sink Terminal - 2
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c27_cyc1
Source - pcr2_denat
Source Terminal - 1
Sink - pcr2_cyc1
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c28_analyte
Source - pcr2_cyc1
Source Terminal - 1
Sink - ch_melt_analysis
Sink Terminal - ERROR!!!!!!
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Connection - c29_done
Source - ch_melt_analysis
Source Terminal - ERROR!!!!!!
Sink - wp
Sink Terminal - 1
channelWidth - 800
channelSpacing - 1600
Adding to FLOW layer ...
Firing Constructor for Placer
---------------Initial Placement Work-----------------
PAR Return code:-11
Rendering 29 components and 30 connections
Place and Route completed with errors, please check the terminal output for information
Activity