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Importing chamber does not allow for design to compile. #9

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watturkara opened this issue Apr 24, 2021 · 0 comments
Open

Importing chamber does not allow for design to compile. #9

watturkara opened this issue Apr 24, 2021 · 0 comments

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@watturkara
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After adding a chamber, the design does not compile because it cannot find proper source/sink terminals from it. Here is the code and error I am getting:

Code:

IMPORT CHAMBER
DEVICE covid_test_circuit

LAYER FLOW


# pm  -> preamplification master mix (input)
# op  -> oligo P solution (input)
# dw -> ultrapure DEPC water (input)
# dm  -> detection master mix (input)
# od  -> oligo D solution (input)
# ps  -> pretreated sample (input)
# wp  -> waste product post melting analysis (output)

PORT pm, op, dw, dm, od, ps, wp portRadius=700;

CHAMBER ch_melt_analysis width=9000 height=250 length=5000;

# Connects different channels together
NODE n1_reagmix, n2_sampmix, n3_reagmix, n4_sampmix, n5_dwsplit, n6_diluter;

# Pumps to control flow rate of DEPC water to 2nd set of reagents and diluter
PUMP pu1_dw_dil, pu2_dw_reag, pu3_pcr1, pu4_pcr2 length=300 width=600 flowChannelWidth=800;

V MIXER preamp_reagmixer;
H MIXER diluter;
V MIXER nested_reagmixer;

# Length/bends assume flow rate of ~ 8.042 uL/min for PCR

V CURVED MIXER pcr1_revt bendLength=8000 numberOfBends=5;
V CURVED MIXER pcr1_stpdn bendLength=8000 numberOfBends=5;
V CURVED MIXER pcr1_denat bendLength=10000 numberOfBends=6;
V CURVED MIXER pcr1_cyc1 bendLength=4500 numberOfBends=20;
V CURVED MIXER pcr1_cyc2 bendLength=30000 numberOfBends=11;
V CURVED MIXER pcr1_finext bendLength=5000 numberOfBends= 4;
V CURVED MIXER pcr2_denat bendLength=10000 numberOfBends=6;
V CURVED MIXER pcr2_cyc1 bendLength=12500 numberOfBends=18;

# Should be incubator/chamber, but could not get this component to work, so
# a curved mixer was used instead
# H CURVED MIXER melt_analysis bendLength=1000 numberOfBends=10;

### PREAMPLIFICATION STEP

## REAGENT PREP

# Split Ultrapure DEPC water via a node
# for use in different parts of the circuit
CHANNEL c0_dw from dw to n5_dwsplit channelWidth=800;

# Join reagents via a node
CHANNEL c1_pm from pm  to n1_reagmix  channelWidth=800;
CHANNEL c2_op from op  to n1_reagmix  channelWidth=800;
CHANNEL c3_dw from n5_dwsplit  to n1_reagmix  channelWidth=800;
# Connect sample to separate node
CHANNEL c4_ps from ps  to n2_sampmix  channelWidth=800;
# Connect reagents node with sample node
CHANNEL c5_combine from n1_reagmix  to n2_sampmix  channelWidth=800;
# Send combined products to mixer 
CHANNEL c6_mix from n2_sampmix  to  preamp_reagmixer  channelWidth=800;

# Pump combined products to target flow rate of 8.042 uL/min
CHANNEL c7_pump from preamp_reagmixer 2 to pu3_pcr1 1 channelWidth=800;

## PERFORM ROUND 1 PCR
# Reverse transcription step
CHANNEL c8_revt from pu3_pcr1 2 to pcr1_revt 2 channelWidth=800;
# Temperature step down step
CHANNEL c9_stpdn from pcr1_revt to pcr1_stpdn  channelWidth=800;
# Initial denaturation step
CHANNEL c10_denat from pcr1_stpdn  to pcr1_denat  channelWidth=800;
# 1st temperature cycle (6 cycles)
CHANNEL c11_cyc1 from pcr1_denat  to pcr1_cyc1 channelWidth=800;
# 2nd temperature cycle (22 cycles)
CHANNEL c12_cyc2 from pcr1_cyc1 to pcr1_cyc2 channelWidth=800;
# Final extension
CHANNEL c13_fin from pcr1_cyc2 to pcr1_finext channelWidth=800;


### NESTED AMPLIFICATION STEP

## REAGENT PREP

# Amplified sample dilution
CHANNEL c14_dil from pcr1_finext to n6_diluter channelWidth=800;
# Pump water from DEPC connect to ensure proper flowrate for dilution
CHANNEL c15_pump from n5_dwsplit to pu1_dw_dil 1 channelWidth=800;
CHANNEL c16_dw from pu1_dw_dil 2 to n6_diluter 1 channelWidth=800;
CHANNEL c17_mix from n6_diluter to diluter channelWidth=800;

# Join reagents via node
CHANNEL c18_dm from dm to n3_reagmix channelWidth=800;
CHANNEL c19_od from od to n3_reagmix channelWidth=800;
# Pump DEPC water to control flowrate for proper mixing ratios
CHANNEL c20_pump from n5_dwsplit 1 to pu2_dw_reag 1 channelWidth=800;
CHANNEL c21_dw from pu2_dw_reag 2 to n3_reagmix 1 channelWidth=800;
# Connect diluted preamp sample to different node
CHANNEL c22_dsamp from diluter to n4_sampmix channelWidth=800;
# Connect reagents node to sample node
CHANNEL c23_combine from n3_reagmix to n4_sampmix channelWidth=800;
# Send combined products to mixer
CHANNEL c24_mix from n4_sampmix to nested_reagmixer channelWidth=800;

# Pump combined products to target flow rate of 8.042 uL/min
CHANNEL c25_pump from nested_reagmixer 2 to pu4_pcr2 1 channelWidth=800;

## PERFORM ROUND 2 PCR
# Inital denaturation step
CHANNEL c26_denat from pu4_pcr2 2 to pcr2_denat 2 channelWidth=800;
# Temperature cycling (45 cycles)
CHANNEL c27_cyc1 from pcr2_denat to pcr2_cyc1 channelWidth=800;

### PERFORM MELTING ANALYSIS
# Move fully amplified sample to melting analysis chamber
CHANNEL c28_analyte from pcr2_cyc1 to ch_melt_analysis 1 channelWidth=800;
# Move analyzed sample to waste product port
CHANNEL c29_done from ch_melt_analysis 2 to wp channelWidth=800;
END LAYER


# LAYER CONTROL
# # Control port for valve
# PORT melt_valve_port portRadius=700;
# # Valves to control fluide flow in/out of melting analysis chamber 
# VALVE melt_valve1 on c28_analyte;
# VALVE melt_valve2 on c29_done;
# # Net to connect valve input to open/close both simultaneously
# NET valve_net from melt_valve_port 1 to melt_valve1 4, melt_valve2 4 channelWidth=50;


# END LAYER

Output:

 _____ _       _       _ 
|  ___| |_   _(_) __ _(_)
| |_  | | | | | |/ _` | |
|  _| | | |_| | | (_| | |
|_|   |_|\__,_|_|\__, |_|
                 |___/   

output dir: /var/www/fluigicad.org/jobs/tmp__6083867ec4667500180b7ad7/output
Running File: /var/www/fluigicad.org/jobs/tmp__6083867ec4667500180b7ad7/ctc2.uf
Creating the output directory:
Computing Constraints
Pulling Default Values of Components
Could not retrieve default parameters for CHAMBER
Expecting value: line 1 column 1 (char 0)
Warning: Could not pull default values for ch_melt_analysis of type :CHAMBER
Pulling Dimensions of Components
Could not retrieve dimensions for CHAMBER
Expecting value: line 1 column 1 (char 0)
Warning: Could not pull default values for ch_melt_analysis of type :CHAMBER
Pulling Terminals of Components
Could not retrieve dimensions for CHAMBER
Expecting value: line 1 column 1 (char 0)
Warning: Could not pull terminal data for ch_melt_analysis of type: CHAMBER
Setting Default MAX Dimensions to the device: (76200, 76200)
Starting terminal/port assignment ...
No port assigned to connection ref - c0_dw:dw:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c0_dw:n5_dwsplit:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c1_pm:pm:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c1_pm:n1_reagmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c2_op:op:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c2_op:n1_reagmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c3_dw:n5_dwsplit:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c3_dw:n1_reagmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c4_ps:ps:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c4_ps:n2_sampmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c5_combine:n1_reagmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c5_combine:n2_sampmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c6_mix:n2_sampmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c6_mix:preamp_reagmixer:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c9_stpdn:pcr1_revt:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c9_stpdn:pcr1_stpdn:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c10_denat:pcr1_stpdn:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c10_denat:pcr1_denat:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c11_cyc1:pcr1_denat:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c11_cyc1:pcr1_cyc1:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c12_cyc2:pcr1_cyc1:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c12_cyc2:pcr1_cyc2:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c13_fin:pcr1_cyc2:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c13_fin:pcr1_finext:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c14_dil:pcr1_finext:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c14_dil:n6_diluter:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c15_pump:n5_dwsplit:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c17_mix:n6_diluter:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c17_mix:diluter:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c18_dm:dm:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c18_dm:n3_reagmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c19_od:od:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c19_od:n3_reagmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c22_dsamp:diluter:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c22_dsamp:n4_sampmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c23_combine:n3_reagmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c23_combine:n4_sampmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c24_mix:n4_sampmix:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
No port assigned to connection ref - c24_mix:nested_reagmixer:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c27_cyc1:pcr2_denat:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c27_cyc1:pcr2_cyc1:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c28_analyte:pcr2_cyc1:None
Auto Port Assign Scheme - Target contains only 2 or 4 port options
Assigned port - 1
No port assigned to connection ref - c29_done:wp:None
Auto Port Assign Scheme - Target contains only 1 port option
Assigned port - 1
output: /var/www/fluigicad.org/jobs/tmp__6083867ec4667500180b7ad7/output/covid_test_circuit.dot
Parsing Layers...
Layer 0:
Name - "FLOW_0"
ID - "0"
Type - "FLOW"
Group - "0"
Component - pm
  xpos - 0
  ypos - 0
  x-span - 1400
  y-span - 1400
  spacing - 1000
  Port - 1
    Port.x - 700
    Port.y - 700
Adding to FLOW ...
Component - op
  xpos - 0
  ypos - 0
  x-span - 1400
  y-span - 1400
  spacing - 1000
  Port - 1
    Port.x - 700
    Port.y - 700
Adding to FLOW ...
Component - dw
  xpos - 0
  ypos - 0
  x-span - 1400
  y-span - 1400
  spacing - 1000
  Port - 1
    Port.x - 700
    Port.y - 700
Adding to FLOW ...
Component - dm
  xpos - 0
  ypos - 0
  x-span - 1400
  y-span - 1400
  spacing - 1000
  Port - 1
    Port.x - 700
    Port.y - 700
Adding to FLOW ...
Component - od
  xpos - 0
  ypos - 0
  x-span - 1400
  y-span - 1400
  spacing - 1000
  Port - 1
    Port.x - 700
    Port.y - 700
Adding to FLOW ...
Component - ps
  xpos - 0
  ypos - 0
  x-span - 1400
  y-span - 1400
  spacing - 1000
  Port - 1
    Port.x - 700
    Port.y - 700
Adding to FLOW ...
Component - wp
  xpos - 0
  ypos - 0
  x-span - 1400
  y-span - 1400
  spacing - 1000
  Port - 1
    Port.x - 700
    Port.y - 700
Adding to FLOW ...
Component - ch_melt_analysis
  xpos - 0
  ypos - 0
  x-span - -1
  y-span - -1
  spacing - 9000
Adding to FLOW ...
Component - n1_reagmix
  xpos - 0
  ypos - 0
  x-span - 800
  y-span - 800
  spacing - 1000
  Port - 1
    Port.x - 0
    Port.y - 0
Adding to FLOW ...
Component - n2_sampmix
  xpos - 0
  ypos - 0
  x-span - 800
  y-span - 800
  spacing - 1000
  Port - 1
    Port.x - 0
    Port.y - 0
Adding to FLOW ...
Component - n3_reagmix
  xpos - 0
  ypos - 0
  x-span - 800
  y-span - 800
  spacing - 1000
  Port - 1
    Port.x - 0
    Port.y - 0
Adding to FLOW ...
Component - n4_sampmix
  xpos - 0
  ypos - 0
  x-span - 800
  y-span - 800
  spacing - 1000
  Port - 1
    Port.x - 0
    Port.y - 0
Adding to FLOW ...
Component - n5_dwsplit
  xpos - 0
  ypos - 0
  x-span - 800
  y-span - 800
  spacing - 1000
  Port - 1
    Port.x - 0
    Port.y - 0
Adding to FLOW ...
Component - n6_diluter
  xpos - 0
  ypos - 0
  x-span - 800
  y-span - 800
  spacing - 1000
  Port - 1
    Port.x - 0
    Port.y - 0
Adding to FLOW ...
Component - pu1_dw_dil
  xpos - 0
  ypos - 0
  x-span - 800
  y-span - 2300
  spacing - 1000
  Port - 1
    Port.x - 400
    Port.y - 0
  Port - 2
    Port.x - 400
    Port.y - 2300
  Port - 3
    Port.x - 400
    Port.y - 150
  Port - 4
    Port.x - 400
    Port.y - 1150
  Port - 5
    Port.x - 400
    Port.y - 2150
Adding to FLOW ...
Component - pu2_dw_reag
  xpos - 0
  ypos - 0
  x-span - 800
  y-span - 2300
  spacing - 1000
  Port - 1
    Port.x - 400
    Port.y - 0
  Port - 2
    Port.x - 400
    Port.y - 2300
  Port - 3
    Port.x - 400
    Port.y - 150
  Port - 4
    Port.x - 400
    Port.y - 1150
  Port - 5
    Port.x - 400
    Port.y - 2150
Adding to FLOW ...
Component - pu3_pcr1
  xpos - 0
  ypos - 0
  x-span - 800
  y-span - 2300
  spacing - 1000
  Port - 1
    Port.x - 400
    Port.y - 0
  Port - 2
    Port.x - 400
    Port.y - 2300
  Port - 3
    Port.x - 400
    Port.y - 150
  Port - 4
    Port.x - 400
    Port.y - 1150
  Port - 5
    Port.x - 400
    Port.y - 2150
Adding to FLOW ...
Component - pu4_pcr2
  xpos - 0
  ypos - 0
  x-span - 800
  y-span - 2300
  spacing - 1000
  Port - 1
    Port.x - 400
    Port.y - 0
  Port - 2
    Port.x - 400
    Port.y - 2300
  Port - 3
    Port.x - 400
    Port.y - 150
  Port - 4
    Port.x - 400
    Port.y - 1150
  Port - 5
    Port.x - 400
    Port.y - 2150
Adding to FLOW ...
Component - preamp_reagmixer
  xpos - 0
  ypos - 0
  x-span - 4060
  y-span - 4860
  spacing - 1000
  Port - 1
    Port.x - 2030
    Port.y - 0
  Port - 2
    Port.x - 2030
    Port.y - 4860
Adding to FLOW ...
Component - diluter
  xpos - 0
  ypos - 0
  x-span - 4060
  y-span - 4860
  spacing - 1000
  Port - 1
    Port.x - 2030
    Port.y - 0
  Port - 2
    Port.x - 2030
    Port.y - 4860
Adding to FLOW ...
Component - nested_reagmixer
  xpos - 0
  ypos - 0
  x-span - 4060
  y-span - 4860
  spacing - 1000
  Port - 1
    Port.x - 2030
    Port.y - 0
  Port - 2
    Port.x - 2030
    Port.y - 4860
Adding to FLOW ...
Component - pcr1_revt
  xpos - 0
  ypos - 0
  x-span - 10830
  y-span - 21100
  spacing - 1000
  Port - 1
    Port.x - 5415
    Port.y - 0
  Port - 2
    Port.x - 5415
    Port.y - 21100
Adding to FLOW ...
Component - pcr1_stpdn
  xpos - 0
  ypos - 0
  x-span - 10830
  y-span - 21100
  spacing - 1000
  Port - 1
    Port.x - 5415
    Port.y - 0
  Port - 2
    Port.x - 5415
    Port.y - 21100
Adding to FLOW ...
Component - pcr1_denat
  xpos - 0
  ypos - 0
  x-span - 12830
  y-span - 25160
  spacing - 1000
  Port - 1
    Port.x - 6415
    Port.y - 0
  Port - 2
    Port.x - 6415
    Port.y - 25160
Adding to FLOW ...
Component - pcr1_cyc1
  xpos - 0
  ypos - 0
  x-span - 7330
  y-span - 82000
  spacing - 1000
  Port - 1
    Port.x - 3665
    Port.y - 0
  Port - 2
    Port.x - 3665
    Port.y - 82000
Adding to FLOW ...
Component - pcr1_cyc2
  xpos - 0
  ypos - 0
  x-span - 32830
  y-span - 45460
  spacing - 1000
  Port - 1
    Port.x - 16415
    Port.y - 0
  Port - 2
    Port.x - 16415
    Port.y - 45460
Adding to FLOW ...
Component - pcr1_finext
  xpos - 0
  ypos - 0
  x-span - 7830
  y-span - 17040
  spacing - 1000
  Port - 1
    Port.x - 3915
    Port.y - 0
  Port - 2
    Port.x - 3915
    Port.y - 17040
Adding to FLOW ...
Component - pcr2_denat
  xpos - 0
  ypos - 0
  x-span - 12830
  y-span - 25160
  spacing - 1000
  Port - 1
    Port.x - 6415
    Port.y - 0
  Port - 2
    Port.x - 6415
    Port.y - 25160
Adding to FLOW ...
Component - pcr2_cyc1
  xpos - 0
  ypos - 0
  x-span - 15330
  y-span - 73880
  spacing - 1000
  Port - 1
    Port.x - 7665
    Port.y - 0
  Port - 2
    Port.x - 7665
    Port.y - 73880
Adding to FLOW ...
Connection - c0_dw
  Source - dw
    Source Terminal - 1
  Sink - n5_dwsplit
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c1_pm
  Source - pm
    Source Terminal - 1
  Sink - n1_reagmix
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c2_op
  Source - op
    Source Terminal - 1
  Sink - n1_reagmix
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c3_dw
  Source - n5_dwsplit
    Source Terminal - 1
  Sink - n1_reagmix
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c4_ps
  Source - ps
    Source Terminal - 1
  Sink - n2_sampmix
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c5_combine
  Source - n1_reagmix
    Source Terminal - 1
  Sink - n2_sampmix
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c6_mix
  Source - n2_sampmix
    Source Terminal - 1
  Sink - preamp_reagmixer
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c7_pump
  Source - preamp_reagmixer
    Source Terminal - 2
  Sink - pu3_pcr1
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c8_revt
  Source - pu3_pcr1
    Source Terminal - 2
  Sink - pcr1_revt
    Sink Terminal - 2
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c9_stpdn
  Source - pcr1_revt
    Source Terminal - 1
  Sink - pcr1_stpdn
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c10_denat
  Source - pcr1_stpdn
    Source Terminal - 1
  Sink - pcr1_denat
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c11_cyc1
  Source - pcr1_denat
    Source Terminal - 1
  Sink - pcr1_cyc1
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c12_cyc2
  Source - pcr1_cyc1
    Source Terminal - 1
  Sink - pcr1_cyc2
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c13_fin
  Source - pcr1_cyc2
    Source Terminal - 1
  Sink - pcr1_finext
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c14_dil
  Source - pcr1_finext
    Source Terminal - 1
  Sink - n6_diluter
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c15_pump
  Source - n5_dwsplit
    Source Terminal - 1
  Sink - pu1_dw_dil
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c16_dw
  Source - pu1_dw_dil
    Source Terminal - 2
  Sink - n6_diluter
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c17_mix
  Source - n6_diluter
    Source Terminal - 1
  Sink - diluter
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c18_dm
  Source - dm
    Source Terminal - 1
  Sink - n3_reagmix
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c19_od
  Source - od
    Source Terminal - 1
  Sink - n3_reagmix
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c20_pump
  Source - n5_dwsplit
    Source Terminal - 1
  Sink - pu2_dw_reag
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c21_dw
  Source - pu2_dw_reag
    Source Terminal - 2
  Sink - n3_reagmix
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c22_dsamp
  Source - diluter
    Source Terminal - 1
  Sink - n4_sampmix
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c23_combine
  Source - n3_reagmix
    Source Terminal - 1
  Sink - n4_sampmix
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c24_mix
  Source - n4_sampmix
    Source Terminal - 1
  Sink - nested_reagmixer
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c25_pump
  Source - nested_reagmixer
    Source Terminal - 2
  Sink - pu4_pcr2
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c26_denat
  Source - pu4_pcr2
    Source Terminal - 2
  Sink - pcr2_denat
    Sink Terminal - 2
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c27_cyc1
  Source - pcr2_denat
    Source Terminal - 1
  Sink - pcr2_cyc1
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c28_analyte
  Source - pcr2_cyc1
    Source Terminal - 1
  Sink - ch_melt_analysis
    Sink Terminal - ERROR!!!!!!
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Connection - c29_done
  Source - ch_melt_analysis
    Source Terminal - ERROR!!!!!!
  Sink - wp
    Sink Terminal - 1
  channelWidth - 800
  channelSpacing - 1600
Adding to FLOW layer ... 
Firing Constructor for Placer
---------------Initial Placement Work-----------------
PAR Return code:-11
Rendering 29 components and 30 connections
Place and Route completed with errors, please check the terminal output for information

@rkrishnasanka rkrishnasanka transferred this issue from CIDARLAB/MINT Jun 19, 2021
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