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a/examples/example_models/dspn/str-dspn-model2/morphology/morphology_hash_filename.json b/examples/example_models/dspn/str-dspn-model2/morphology/morphology_hash_filename.json deleted file mode 100644 index af320fbe..00000000 --- a/examples/example_models/dspn/str-dspn-model2/morphology/morphology_hash_filename.json +++ /dev/null @@ -1,11 +0,0 @@ -{ - "m01df38e6": "21-6-DE-cor-rep-ax-res3-var3.swc", - "m1f69c32c": "21-6-DE-cor-rep-ax-res3-var6.swc", - "m762b309f": "21-6-DE-cor-rep-ax-res3-var4.swc", - "m7721d5cf": "21-6-DE-cor-rep-ax-res3-var5.swc", - "m91d5391a": "21-6-DE-cor-rep-ax-res3-var2.swc", - "m93f282f3": "21-6-DE-cor-rep-ax-res3-var0.swc", - "md9d948b6": "21-6-DE-cor-rep-ax-res3-var8.swc", - "mdf60297d": "21-6-DE-cor-rep-ax-res3-var1.swc", - "mf0350288": "21-6-DE-cor-rep-ax-res3-var7.swc" -} \ No newline at end of file diff --git a/examples/example_models/dspn/str-dspn-model2/parameters.json b/examples/example_models/dspn/str-dspn-model2/parameters.json deleted file mode 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-38.2779 52.4778 -12.9128 0.075 1705 -1709 2 -40.01 56.81 -13.6267 0.075 1708 -1710 2 -38.7336 58.6405 -16.0145 0.075 1709 -1711 2 -37.9455 60.8695 -18.2701 0.075 1710 -1712 2 -36.847 63.6438 -19.5768 0.075 1711 -1713 2 -35.6765 66.5036 -20.637 0.075 1712 -1714 2 -34.6189 69.0933 -22.1852 0.075 1713 -1715 2 -33.5881 70.1164 -25.0883 0.075 1714 -1716 2 -31.5694 72.0569 -26.7636 0.075 1715 -1717 2 -29.285 73.9752 -28.0865 0.075 1716 -1718 2 -26.8872 75.0999 -29.9304 0.075 1717 -1719 2 -24.4581 75.5761 -32.0383 0.075 1718 -1720 2 -21.42 75.3 -32.8933 0.075 1719 -1721 2 -41.7062 60.144 -13.5745 0.075 1709 -1722 2 -42.4438 63.454 -12.1249 0.075 1721 -1723 2 -43.4167 66.783 -10.8295 0.075 1722 -1724 2 -45.17 70.04 -11.0933 0.075 1723 -1725 2 -48.4217 70.0526 -10.6587 0.075 1724 -1726 2 -50.8929 69.8093 -12.8286 0.075 1725 -1727 2 -53.8272 69.2509 -12.8866 0.075 1726 -1728 2 -56.7555 68.5436 -11.2532 0.075 1727 -1729 2 -59.8678 67.0282 -10.8641 0.075 1728 -1730 2 -62.93 65.54 -11.32 0.075 1729 -1731 2 -44.9881 72.7908 -11.9233 0.075 1724 -1732 2 -45.8447 75.1487 -13.5793 0.075 1731 -1733 2 -46.7474 77.5301 -15.0209 0.075 1732 -1734 2 -47.5017 80.4656 -15.4295 0.075 1733 -1735 2 -48.1267 83.4359 -15.791 0.075 1734 -1736 2 -48.8274 86.3846 -16.1685 0.075 1735 -1737 2 -49.5461 89.3357 -16.5038 0.075 1736 -1738 2 -50.3463 92.0821 -17.5083 0.075 1737 -1739 2 -50.9171 94.8634 -18.6435 0.075 1738 -1740 2 -51.6393 97.6821 -19.5731 0.075 1739 -1741 2 -52.2205 100.543 -20.4313 0.075 1740 -1742 2 -52.6788 103.495 -20.5188 0.075 1741 -1743 2 -53.4503 106.396 -19.9474 0.075 1742 -1744 2 -54.1357 109.352 -19.9577 0.075 1743 -1745 2 -54.3142 112.348 -20.3903 0.075 1744 -1746 2 -53.6944 115.26 -21.0772 0.075 1745 -1747 2 -53.6383 118.222 -21.7063 0.075 1746 -1748 2 -54.3952 121.159 -22.0629 0.075 1747 -1749 2 -55.2213 123.923 -21.2735 0.075 1748 -1750 2 -56.3428 126.633 -20.4169 0.075 1749 -1751 2 -57.5935 129.395 -20.4492 0.075 1750 -1752 2 -58.8381 131.987 -21.3773 0.075 1751 -1753 2 -60.0508 134.291 -22.966 0.075 1752 -1754 2 -60.5768 136.276 -25.2271 0.075 1753 -1755 2 -61.8036 136.93 -27.9209 0.075 1754 -1756 2 -63.6531 137.295 -30.3257 0.075 1755 -1757 2 -65.1167 138.187 -32.8135 0.075 1756 -1758 2 -65.8716 140.246 -34.8543 0.075 1757 -1759 2 -66.9145 142.838 -35.9285 0.075 1758 -1760 2 -67.5537 145.497 -37.2912 0.075 1759 -1761 2 -68.11 148.23 -38.5067 0.075 1760 -1762 2 -16.9332 -25.5453 1.13818 0.075 1644 -1763 2 -17.4451 -22.202 0.754227 0.075 1762 -1764 2 -17.1532 -18.8251 0.443113 0.075 1763 -1765 2 -16.557 -15.4669 0.382047 0.075 1764 -1766 2 -16.3614 -12.0995 0.875307 0.075 1765 -1767 2 -15.4627 -8.84892 0.86798 0.075 1766 -1768 2 -14.1879 -5.72288 0.395653 0.075 1767 -1769 2 -14.1425 -2.38547 -0.13839 0.075 1768 -1770 2 -14.8018 0.888141 -0.809309 0.075 1769 -1771 2 -14.95 4.2 -1.61333 0.075 1770 -1772 2 -17.2488 5.11942 -3.11633 0.075 1771 -1773 2 -20.2399 4.98677 -3.82171 0.075 1772 -1774 2 -22.7729 5.33865 -5.60534 0.075 1773 -1775 2 -25.1708 6.17149 -7.43209 0.075 1774 -1776 2 -27.5599 7.55068 -8.88198 0.075 1775 -1777 2 -30.1092 9.3198 -9.29191 0.075 1776 -1778 2 -32.2555 11.2474 -8.56222 0.075 1777 -1779 2 -33.888 12.6539 -10.3934 0.075 1778 -1780 2 -35.5953 14.028 -12.6364 0.075 1779 -1781 2 -37.6621 15.566 -14.0946 0.075 1780 -1782 2 -39.8872 17.7704 -14.2104 0.075 1781 -1783 2 -42.2034 19.8827 -14.1209 0.075 1782 -1784 2 -44.573 21.9319 -13.9923 0.075 1783 -1785 2 -46.7304 24.1952 -14.0853 0.075 1784 -1786 2 -49.08 26.25 -14.3333 0.075 1785 -1787 2 -13.7549 6.05889 -0.373231 0.075 1771 -1788 2 -13.4813 6.74221 -3.00339 0.075 1787 -1789 2 -13.2783 7.40039 -6.03165 0.075 1788 -1790 2 -12.9 9.07003 -8.28374 0.075 1789 -1791 2 -12.3388 11.8957 -9.35789 0.075 1790 -1792 2 -11.2306 14.7558 -9.80351 0.075 1791 -1793 2 -10.1454 17.6149 -10.2862 0.075 1792 -1794 2 -9.53662 20.5831 -10.9451 0.075 1793 -1795 2 -8.5795 23.4166 -11.7398 0.075 1794 -1796 2 -7.25307 26.016 -12.612 0.075 1795 -1797 2 -6.73299 28.3632 -10.6775 0.075 1796 -1798 2 -5.92086 30.6107 -8.86367 0.075 1797 -1799 2 -4.46765 33.2658 -8.18158 0.075 1798 -1800 2 -3.01693 35.9404 -8.69977 0.075 1799 -1801 2 -2.1108 38.79 -9.45711 0.075 1800 -1802 2 -1.84566 41.7519 -9.11822 0.075 1801 -1803 2 -1.88799 43.9365 -7.00117 0.075 1802 -1804 2 -1.51178 45.7817 -4.53167 0.075 1803 -1805 2 -1.13557 47.627 -2.06218 0.075 1804 -1806 2 -0.32342 50.4988 -1.44201 0.075 1805 -1807 2 0.616661 53.445 -1.70472 0.075 1806 -1808 2 2.36951 55.94 -1.9941 0.075 1807 -1809 2 4.57538 58.12 -2.05558 0.075 1808 -1810 2 6.67357 60.3836 -2.18785 0.075 1809 -1811 2 8.5954 62.6389 -3.0945 0.075 1810 -1812 2 10.7947 64.5938 -4.05512 0.075 1811 -1813 2 13.2625 66.4127 -4.08514 0.075 1812 -1814 2 15.0715 68.6895 -3.12616 0.075 1813 -1815 2 16.4935 71.3029 -2.36779 0.075 1814 -1816 2 18.6814 73.4191 -2.56908 0.075 1815 -1817 2 21.2424 75.0888 -3.08135 0.075 1816 -1818 2 23.7115 76.5869 -4.16382 0.075 1817 -1819 2 25.6958 78.5496 -5.51046 0.075 1818 -1820 2 27.7066 80.6136 -6.66421 0.075 1819 -1821 2 29.7143 82.7713 -7.63285 0.075 1820 -1822 2 31.6224 85.0793 -8.44985 0.075 1821 -1823 2 32.9829 87.5357 -9.71348 0.075 1822 -1824 2 34.0517 89.854 -11.4782 0.075 1823 -1825 2 35.3809 92.0954 -13.1619 0.075 1824 -1826 2 36.7797 94.2754 -14.8532 0.075 1825 -1827 2 37.8122 96.4286 -16.8267 0.075 1826 -1828 2 38.24 98.96 -18.52 0.075 1827 -1829 2 -4.98473 -55.0841 5.87811 0.075 1581 -1830 2 -1.01111 -54.767 7.94713 0.075 1829 -1831 2 2.45 -54.96 10.6133 0.075 1830 -1832 2 40.3231 -151.19 21.3352 0.075 1352 -1833 2 39.5815 -153.979 22.5053 0.075 1832 -1834 2 40.0979 -156.871 23.606 0.075 1833 -1835 2 41.2113 -159.11 25.5344 0.075 1834 -1836 2 42.6146 -161.241 27.452 0.075 1835 -1837 2 44.3127 -163.613 28.6243 0.075 1836 -1838 2 46.4125 -166.015 28.7903 0.075 1837 -1839 2 48.55 -168.38 28.9467 0.075 1838 -1840 2 49.1145 -171.421 29.3838 0.075 1839 -1841 2 49.3497 -174.502 29.836 0.075 1840 -1842 2 49.3259 -176.233 32.3103 0.075 1841 -1843 2 49.2669 -177.84 34.9906 0.075 1842 -1844 2 50.1356 -179.837 37.1624 0.075 1843 -1845 2 51.2331 -182.32 38.6508 0.075 1844 -1846 2 52.2061 -184.135 40.8512 0.075 1845 -1847 2 53.2077 -185.717 43.3541 0.075 1846 -1848 2 54.0067 -187.671 45.5738 0.075 1847 -1849 2 54.3849 -190.282 47.1404 0.075 1848 -1850 2 54.3907 -193.346 47.7259 0.075 1849 -1851 2 54.9347 -196.132 48.8875 0.075 1850 -1852 2 55.703 -198.221 51.0742 0.075 1851 -1853 2 56.0755 -200.191 53.4514 0.075 1852 -1854 2 55.9355 -202.081 55.9204 0.075 1853 -1855 2 55.3108 -203.946 58.3378 0.075 1854 -1856 2 54.9003 -205.313 61.0456 0.075 1855 -1857 2 54.9 -205.68 64.1333 0.075 1856 -1858 2 51.4498 -168.749 28.7962 0.075 1839 -1859 2 53.9769 -170.4 29.0304 0.075 1858 -1860 2 56.1618 -171.479 30.887 0.075 1859 -1861 2 58.0878 -172.435 33.0699 0.075 1860 -1862 2 59.749 -174.858 33.8708 0.075 1861 -1863 2 61.394 -177.424 34.1479 0.075 1862 -1864 2 63.181 -179.909 34.454 0.075 1863 -1865 2 64.8105 -182.454 34.9875 0.075 1864 -1866 2 66.5453 -184.783 35.9937 0.075 1865 -1867 2 68.3277 -186.846 37.3654 0.075 1866 -1868 2 69.1883 -189.258 39.0689 0.075 1867 -1869 2 69.9652 -191.701 40.7812 0.075 1868 -1870 2 70.6687 -193.886 42.8028 0.075 1869 -1871 2 71.7626 -195.981 44.7265 0.075 1870 -1872 2 73.4304 -198.321 45.7929 0.075 1871 -1873 2 75.4669 -200.613 46.0827 0.075 1872 -1874 2 77.7096 -202.687 46.4725 0.075 1873 -1875 2 80.0116 -204.632 47.1207 0.075 1874 -1876 2 82.4716 -206.193 48.1155 0.075 1875 -1877 2 84.8303 -207.876 49.1421 0.075 1876 -1878 2 87.0137 -209.798 50.148 0.075 1877 -1879 2 89.0233 -212.106 50.4965 0.075 1878 -1880 2 91.9234 -213.11 50.4698 0.075 1879 -1881 2 94.9714 -213.506 50.7021 0.075 1880 -1882 2 97.921 -213.203 51.4071 0.075 1881 -1883 2 100.819 -212.991 52.3707 0.075 1882 -1884 2 103.758 -213.551 53.0533 0.075 1883 -1885 2 106.681 -214.089 53.7997 0.075 1884 -1886 2 108.92 -215.178 55.6148 0.075 1885 -1887 2 111.087 -216.338 57.4724 0.075 1886 -1888 2 113.446 -217.102 59.3028 0.075 1887 -1889 2 116.034 -217.694 60.8201 0.075 1888 -1890 2 118.874 -218.131 61.9239 0.075 1889 -1891 2 121.171 -218.221 63.4944 0.075 1890 -1892 2 122.07 -217.9 66.4133 0.075 1891 -1893 2 51.7237 -150.846 24.2375 0.075 1349 -1894 2 54.5459 -152.737 24.8602 0.075 1893 -1895 2 57.5622 -153.649 25.4262 0.075 1894 -1896 2 60.4483 -153.405 26.768 0.075 1895 -1897 2 63.8326 -153.971 26.3205 0.075 1896 -1898 2 67.2785 -153.921 26.2526 0.075 1897 -1899 2 70.6817 -153.441 26.3726 0.075 1898 -1900 2 73.95 -153.29 27.4267 0.075 1899 -1901 2 74.2394 -154.986 30.0471 0.075 1900 -1902 2 74.6745 -156.897 32.4737 0.075 1901 -1903 2 75.2788 -158.987 34.7264 0.075 1902 -1904 2 76.575 -161.213 36.4086 0.075 1903 -1905 2 78.0734 -163.46 37.9776 0.075 1904 -1906 2 79.2894 -165.787 39.6768 0.075 1905 -1907 2 80.3595 -168.042 41.4213 0.075 1906 -1908 2 80.9773 -169.24 44.1749 0.075 1907 -1909 2 81.3846 -170.152 47.1464 0.075 1908 -1910 2 82.1006 -171.618 49.7682 0.075 1909 -1911 2 82.652 -173.283 52.2308 0.075 1910 -1912 2 82.84 -174.25 55.2 0.075 1911 -1913 2 76.8492 -152.28 25.0512 0.075 1900 -1914 2 79.5186 -150.708 22.6592 0.075 1913 -1915 2 82.04 -149.26 20.0267 0.075 1914 -1916 2 81.7129 -153.556 20.106 0.075 1915 -1917 2 81.4853 -157.46 21.2007 0.075 1916 -1918 2 81.77 -159.54 24.9333 0.075 1917 -1919 2 84.158 -147.107 19.2238 0.075 1915 -1920 2 85.8695 -145.037 17.5338 0.075 1919 -1921 2 88.5009 -143.596 18.0729 0.075 1920 -1922 2 91.3457 -142.758 19.1899 0.075 1921 -1923 2 94.057 -141.905 20.5652 0.075 1922 -1924 2 96.5927 -141.784 22.4331 0.075 1923 -1925 2 98.6357 -141.777 21.7531 0.075 1924 -1926 2 99.1769 -140.818 18.8137 0.075 1925 -1927 2 100.774 -139.912 16.4344 0.075 1926 -1928 2 103.766 -139.286 15.8472 0.075 1927 -1929 2 106.621 -137.95 16.0329 0.075 1928 -1930 2 108.735 -136.726 17.9031 0.075 1929 -1931 2 111.16 -135.9 19.56 0.075 1930 -1932 2 113.451 -137.38 20.5556 0.075 1931 -1933 2 115.031 -139.741 21.6485 0.075 1932 -1934 2 116.159 -142.57 22.0109 0.075 1933 -1935 2 117.274 -145.399 22.4085 0.075 1934 -1936 2 118.482 -147.967 23.1342 0.075 1935 -1937 2 119.383 -149.467 20.6107 0.075 1936 -1938 2 120.283 -150.967 18.0872 0.075 1937 -1939 2 121.093 -152.941 16.1839 0.075 1938 -1940 2 121.936 -155.818 15.5501 0.075 1939 -1941 2 123.333 -158.274 14.5518 0.075 1940 -1942 2 124.749 -160.313 12.7603 0.075 1941 -1943 2 126.082 -162.371 10.9158 0.075 1942 -1944 2 127.534 -164.634 9.46021 0.075 1943 -1945 2 129.201 -166.912 8.25263 0.075 1944 -1946 2 130.978 -169.134 7.11074 0.075 1945 -1947 2 132.672 -171.205 5.62064 0.075 1946 -1948 2 133.629 -173.889 4.63161 0.075 1947 -1949 2 134.692 -176.751 4.84525 0.075 1948 -1950 2 136.253 -179.325 5.23669 0.075 1949 -1951 2 139.109 -180.245 4.67024 0.075 1950 -1952 2 141.876 -181.182 3.7287 0.075 1951 -1953 2 144.681 -182.303 3.51865 0.075 1952 -1954 2 147.324 -183.468 4.54506 0.075 1953 -1955 2 150.07 -184.66 5.05333 0.075 1954 -1956 2 113.371 -133.621 19.1406 0.075 1931 -1957 2 116.258 -132.087 18.7601 0.075 1956 -1958 2 118.743 -129.888 18.3214 0.075 1957 -1959 2 121.439 -127.939 17.9174 0.075 1958 -1960 2 124.503 -127.171 17.1858 0.075 1959 -1961 2 126.82 -125.04 16.44 0.075 1960 -1962 2 127.771 -123.622 19.0823 0.075 1961 -1963 2 129.217 -121.619 20.9836 0.075 1962 -1964 2 130.776 -119.68 22.8945 0.075 1963 -1965 2 132.76 -118.197 24.8098 0.075 1964 -1966 2 134.05 -116.241 26.8603 0.075 1965 -1967 2 134.481 -113.982 28.9478 0.075 1966 -1968 2 134.754 -111.474 30.8171 0.075 1967 -1969 2 135.097 -108.68 32.1248 0.075 1968 -1970 2 135.245 -105.771 33.3003 0.075 1969 -1971 2 136.075 -103.225 34.7475 0.075 1970 -1972 2 137.721 -101.537 36.8217 0.075 1971 -1973 2 139.169 -99.8642 38.9663 0.075 1972 -1974 2 140.023 -98.3674 41.5983 0.075 1973 -1975 2 140.988 -96.5497 43.7521 0.075 1974 -1976 2 142.317 -94.0187 44.6866 0.075 1975 -1977 2 144.079 -91.9014 46.206 0.075 1976 -1978 2 145.205 -89.3173 46.8257 0.075 1977 -1979 2 145.329 -86.2015 46.8461 0.075 1978 -1980 2 145.266 -83.3128 47.8116 0.075 1979 -1981 2 145.331 -81.1619 50.05 0.075 1980 -1982 2 145.236 -80.2881 47.6726 0.075 1981 -1983 2 145.106 -79.5364 44.6204 0.075 1982 -1984 2 144.85 -78.4618 41.7648 0.075 1983 -1985 2 144.199 -76.4441 39.4432 0.075 1984 -1986 2 143.296 -73.8445 37.9225 0.075 1985 -1987 2 142.339 -71.0032 37.0682 0.075 1986 -1988 2 141.393 -68.1376 36.1885 0.075 1987 -1989 2 140.362 -65.5226 34.8173 0.075 1988 -1990 2 139.19 -63.16 33.1067 0.075 1989 -1991 2 139.586 -60.2938 34.8884 0.075 1990 -1992 2 138.237 -57.8984 33.2723 0.075 1991 -1993 2 137.117 -55.4581 31.2076 0.075 1992 -1994 2 136.256 -53.442 28.6337 0.075 1993 -1995 2 135.306 -51.2601 26.2292 0.075 1994 -1996 2 134.631 -49.0255 23.7961 0.075 1995 -1997 2 134.03 -46.69 21.4933 0.075 1996 -1998 2 139.257 -63.2432 36.1809 0.075 1990 -1999 2 139.323 -63.3264 39.2551 0.075 1998 -2000 2 139.39 -63.4095 42.3293 0.075 1999 -2001 2 137.591 -65.6782 43.3183 0.075 2000 -2002 2 135.76 -67.0277 45.2026 0.075 2001 -2003 2 134.377 -67.8628 47.8194 0.075 2002 -2004 2 132.71 -68.6929 50.2221 0.075 2003 -2005 2 130.239 -69.8416 51.4581 0.075 2004 -2006 2 127.525 -71.1759 52.0072 0.075 2005 -2007 2 125.782 -72.2079 54.1104 0.075 2006 -2008 2 124.935 -73.0556 56.9246 0.075 2007 -2009 2 124.546 -74.2363 59.6381 0.075 2008 -2010 2 124.924 -76.2456 61.8926 0.075 2009 -2011 2 126.265 -78.9207 62.5796 0.075 2010 -2012 2 127.486 -81.1911 64.235 0.075 2011 -2013 2 127.732 -84.0269 65.2897 0.075 2012 -2014 2 127.529 -87.0695 65.688 0.075 2013 -2015 2 127.55 -89.7111 66.967 0.075 2014 -2016 2 127.589 -91.9962 69.026 0.075 2015 -2017 2 127.652 -94.3092 71.0383 0.075 2016 -2018 2 128.121 -97.0815 72.2858 0.075 2017 -2019 2 128.7 -99.99 73 0.075 2018 -2020 2 131.11 -123.81 15.9333 0.075 1961 -2021 2 132.189 -125.316 12.8626 0.075 2020 -2022 2 133.298 -128.062 10.8598 0.075 2021 -2023 2 134.159 -130.819 8.74254 0.075 2022 -2024 2 136.011 -132.441 6.30638 0.075 2023 -2025 2 136.178 -135.139 4.30202 0.075 2024 -2026 2 135.53 -137.86 2.06667 0.075 2025 -2027 2 133.963 -122.675 15.6687 0.075 2020 -2028 2 136.929 -121.849 15.5195 0.075 2027 -2029 2 139.181 -119.772 15.1146 0.075 2028 -2030 2 140.898 -117.262 14.4545 0.075 2029 -2031 2 143.263 -115.369 14.6026 0.075 2030 -2032 2 145.873 -114.112 13.8982 0.075 2031 -2033 2 147.681 -112.843 11.9165 0.075 2032 -2034 2 148.147 -110.303 10.3088 0.075 2033 -2035 2 150.293 -108.226 9.92323 0.075 2034 -2036 2 152.937 -106.642 9.7047 0.075 2035 -2037 2 155.471 -104.856 9.42381 0.075 2036 -2038 2 157.747 -103.117 8.43085 0.075 2037 -2039 2 159.557 -101.791 6.30821 0.075 2038 -2040 2 161.604 -100.535 4.34113 0.075 2039 -2041 2 164.324 -99.0559 4.08618 0.075 2040 -2042 2 167.135 -98.3239 5.11324 0.075 2041 -2043 2 170.185 -98.3299 5.55466 0.075 2042 -2044 2 173.274 -98.3593 5.39828 0.075 2043 -2045 2 176.239 -97.5016 5.0985 0.075 2044 -2046 2 179.298 -96.9541 4.85044 0.075 2045 -2047 2 182.379 -96.5254 4.75357 0.075 2046 -2048 2 185.24 -96.1714 3.99922 0.075 2047 -2049 2 188.005 -95.515 2.78605 0.075 2048 -2050 2 190.563 -94.0855 1.94545 0.075 2049 -2051 2 193.02 -92.22 1.49333 0.075 2050 -2052 2 192.804 -91.1606 -1.58252 0.075 2051 -2053 2 191.982 -90.2555 -4.55321 0.075 2052 -2054 2 190.668 -89.3578 -7.39186 0.075 2053 -2055 2 189.435 -88.306 -10.218 0.075 2054 -2056 2 188.201 -87.3545 -13.0658 0.075 2055 -2057 2 186.63 -86.368 -15.7279 0.075 2056 -2058 2 185.454 -84.579 -18.0087 0.075 2057 -2059 2 185.6 -81.65 -19.2667 0.075 2058 -2060 2 187.936 -79.5351 -20.8382 0.075 2059 -2061 2 188.84 -77.6961 -23.589 0.075 2060 -2062 2 188.426 -76.0965 -26.7258 0.075 2061 -2063 2 187.813 -73.9982 -29.5181 0.075 2062 -2064 2 188.31 -71.89 -32.24 0.075 2063 -2065 2 184.61 -80.862 -22.158 0.075 2059 -2066 2 183.725 -79.5729 -24.8163 0.075 2065 -2067 2 183.063 -77.2506 -26.7824 0.075 2066 -2068 2 181.924 -74.3756 -27.2847 0.075 2067 -2069 2 181.054 -71.3922 -27.6869 0.075 2068 -2070 2 181.129 -68.3011 -28.1755 0.075 2069 -2071 2 181.995 -65.3113 -28.2311 0.075 2070 -2072 2 182.436 -62.345 -27.7367 0.075 2071 -2073 2 182.523 -59.2241 -27.5468 0.075 2072 -2074 2 182.184 -56.1745 -26.9654 0.075 2073 -2075 2 181.357 -53.2646 -26.8889 0.075 2074 -2076 2 182.032 -50.2086 -26.7903 0.075 2075 -2077 2 182 -47.2131 -26.4792 0.075 2076 -2078 2 180.961 -44.4032 -26.8239 0.075 2077 -2079 2 180.849 -41.2889 -27.3102 0.075 2078 -2080 2 180.818 -38.1583 -27.6468 0.075 2079 -2081 2 180.611 -35.0257 -27.4302 0.075 2080 -2082 2 180.002 -33.5417 -29.556 0.075 2081 -2083 2 179.38 -32.53 -32.48 0.075 2082 -2084 2 197.64 -91.55 4.72 0.075 2051 -2085 2 201.387 -92.5143 2.6674 0.075 2084 -2086 2 205.95 -92.15 2.33333 0.075 2085 -2087 2 201.209 -90.1662 4.71335 0.075 2084 -2088 2 204.913 -90.2038 5.37118 0.075 2087 -2089 2 208.148 -88.7244 6.80994 0.075 2088 -2090 2 211.734 -87.7826 6.20657 0.075 2089 -2091 2 214.61 -86.14 4.37333 0.075 2090 -2092 2 215.564 -84.3174 3.31335 0.075 2091 -2093 2 216.846 -83.3338 6.84553 0.075 2092 -2094 2 218.37 -81.9464 9.74047 0.075 2093 -2095 2 219.57 -78.3351 9.71115 0.075 2094 -2096 2 219.52 -74.48 9.96 0.075 2095 -2097 2 219.61 -71.118 10.4655 0.075 2096 -2098 2 220.58 -67.8942 10.003 0.075 2097 -2099 2 220.877 -64.5276 9.81841 0.075 2098 -2100 2 221.167 -61.369 10.78 0.075 2099 -2101 2 221.83 -58.42 12.3333 0.075 2100 -2102 2 220.232 -73.7108 13.3044 0.075 2096 -2103 2 220.944 -72.9416 16.6488 0.075 2102 -2104 2 221.64 -71.7138 14.0669 0.075 2103 -2105 2 222.2 -70.39 10.88 0.075 2104 -2106 2 216.772 -87.1516 2.05531 0.075 2091 -2107 2 217.803 -87.1479 -1.45776 0.075 2106 -2108 2 218.687 -88.1719 -4.63867 0.075 2107 -2109 2 219.006 -87.3473 -8.27179 0.075 2108 -2110 2 221.05 -87.72 -10.92 0.075 2109 -2111 2 223.761 -88.7587 -11.0599 0.075 2110 -2112 2 226.155 -90.1878 -9.66104 0.075 2111 -2113 2 227.97 -92.4106 -8.44188 0.075 2112 -2114 2 229.75 -93.4983 -6.31203 0.075 2113 -2115 2 231.484 -93.5719 -3.70139 0.075 2114 -2116 2 233.779 -93.8474 -1.70923 0.075 2115 -2117 2 236.444 -95.3585 -1.48474 0.075 2116 -2118 2 238.941 -97.1058 -0.91967 0.075 2117 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0.075 2140 -2142 2 228.574 -109.489 -11.1753 0.075 2141 -2143 2 226.25 -111.56 -10.36 0.075 2142 -2144 2 221.29 -83.4699 -23.6764 0.075 2133 -2145 2 222.01 -82.5227 -26.6391 0.075 2144 -2146 2 223.957 -81.6281 -27.8811 0.075 2145 -2147 2 226.275 -80.1232 -28.6723 0.075 2146 -2148 2 227.994 -80.2582 -31.4774 0.075 2147 -2149 2 229.082 -79.6244 -34.4808 0.075 2148 -2150 2 230.179 -77.5819 -36.7807 0.075 2149 -2151 2 231.514 -74.7611 -37.9026 0.075 2150 -2152 2 233.482 -72.3231 -38.9688 0.075 2151 -2153 2 235.55 -70.82 -40.96 0.075 2152 -2154 2 36.1649 -124.438 23.3514 0.075 1341 -2155 2 34.7443 -122.675 21.2092 0.075 2154 -2156 2 33.156 -120.839 19.2382 0.075 2155 -2157 2 31.2058 -118.812 17.9709 0.075 2156 -2158 2 28.7616 -117.219 16.8395 0.075 2157 -2159 2 26.6907 -115.377 15.4167 0.075 2158 -2160 2 24.5046 -114.423 13.5449 0.075 2159 -2161 2 21.8935 -112.765 13.1467 0.075 2160 -2162 2 19.0228 -111.737 13.7936 0.075 2161 -2163 2 16.0457 -110.988 14.4016 0.075 2162 -2164 2 12.9838 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-110.789 37.7579 0.075 2186 -2188 2 59.1769 -111.778 39.2779 0.075 2187 -2189 2 61.35 -114.78 41.2667 0.075 2188 -2190 2 65.3494 -115.176 41.736 0.075 2189 -2191 2 69.17 -116.48 42.0533 0.075 2190 -2192 2 71.8186 -117.235 43.3755 0.075 2191 -2193 2 74.1648 -118.682 44.1936 0.075 2192 -2194 2 76.1214 -121.022 44.3622 0.075 2193 -2195 2 77.6227 -123.657 44.5945 0.075 2194 -2196 2 79.2367 -126.235 44.813 0.075 2195 -2197 2 81.0921 -128.643 45.0872 0.075 2196 -2198 2 82.7012 -131.203 45.4657 0.075 2197 -2199 2 84.8066 -133.359 45.4566 0.075 2198 -2200 2 87.2696 -135.115 45.2062 0.075 2199 -2201 2 89.7902 -136.734 45.284 0.075 2200 -2202 2 91.8191 -138.855 45.9943 0.075 2201 -2203 2 93.4454 -141.352 46.6186 0.075 2202 -2204 2 95.178 -143.835 46.8436 0.075 2203 -2205 2 97.3916 -145.926 47.0891 0.075 2204 -2206 2 99.5883 -148.021 47.4295 0.075 2205 -2207 2 101.644 -150.054 48.328 0.075 2206 -2208 2 103.428 -151.913 49.8401 0.075 2207 -2209 2 105.581 -154.005 50.4056 0.075 2208 -2210 2 107.735 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55.9724 0.075 2278 -2280 2 92.343 -88.9824 58.4738 0.075 2279 -2281 2 94.0832 -90.3112 60.2945 0.075 2280 -2282 2 96.0065 -92.7494 60.4747 0.075 2281 -2283 2 97.7066 -95.2292 60.9619 0.075 2282 -2284 2 99.4102 -97.0993 62.737 0.075 2283 -2285 2 101.658 -98.3651 64.465 0.075 2284 -2286 2 104.557 -99.4654 64.7385 0.075 2285 -2287 2 107.352 -100.771 64.9189 0.075 2286 -2288 2 109.597 -102.63 65.9364 0.075 2287 -2289 2 111.384 -104.808 67.245 0.075 2288 -2290 2 112.129 -106.582 65.7459 0.075 2289 -2291 2 112.539 -108.218 63.1294 0.075 2290 -2292 2 113.184 -110.126 61.1762 0.075 2291 -2293 2 114.749 -112.757 60.7707 0.075 2292 -2294 2 116.214 -115.251 59.6636 0.075 2293 -2295 2 117.947 -117.794 59.3171 0.075 2294 -2296 2 119.997 -120.132 59.4273 0.075 2295 -2297 2 122.019 -122.497 59.5142 0.075 2296 -2298 2 123.946 -124.941 59.5187 0.075 2297 -2299 2 125.8 -127.44 59.5187 0.075 2298 -2300 2 127.252 -130.18 59.6503 0.075 2299 -2301 2 128.459 -133.042 59.8468 0.075 2300 -2302 2 129.988 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-27.8411 0.075 1827 -1829 2 -32.4382 78.1606 -29.3735 0.075 1828 -1830 2 -30.13 79.856 -30.8346 0.075 1829 -1831 2 -27.718 80.8701 -32.7319 0.075 1830 -1832 2 -25.2437 81.2886 -34.743 0.075 1831 -1833 2 -22.1338 81.0059 -35.6182 0.075 1832 -1834 2 -44.2007 64.4051 -14.5147 0.075 1821 -1835 2 -45.0092 67.9841 -12.955 0.075 1834 -1836 2 -46.0763 71.5675 -11.5985 0.075 1835 -1837 2 -47.9616 75.0696 -11.8822 0.075 1836 -1838 2 -51.4494 75.059 -11.6549 0.075 1837 -1839 2 -54.2099 74.7616 -13.8399 0.075 1838 -1840 2 -57.6659 74.0779 -13.6156 0.075 1839 -1841 2 -60.7548 73.24 -12.0547 0.075 1840 -1842 2 -63.967 71.6761 -11.6978 0.075 1841 -1843 2 -67.1724 70.1183 -12.175 0.075 1842 -1844 2 -47.8485 78.0097 -12.8451 0.075 1837 -1845 2 -48.7389 80.459 -14.561 0.075 1844 -1846 2 -49.7015 82.9982 -16.098 0.075 1845 -1847 2 -50.4737 85.9869 -16.5306 0.075 1846 -1848 2 -51.1239 89.0156 -16.904 0.075 1847 -1849 2 -51.8265 92.0314 -17.2869 0.075 1848 -1850 2 -52.556 95.0446 -17.6399 0.075 1849 -1851 2 -53.3505 97.9317 -18.45 0.075 1850 -1852 2 -54.0276 100.765 -19.5596 0.075 1851 -1853 2 -54.6923 103.625 -20.6073 0.075 1852 -1854 2 -55.3658 106.529 -21.5254 0.075 1853 -1855 2 -55.9172 109.519 -22.1057 0.075 1854 -1856 2 -56.4967 112.562 -21.9767 0.075 1855 -1857 2 -57.2638 115.544 -21.5413 0.075 1856 -1858 2 -57.875 118.592 -21.63 0.075 1857 -1859 2 -57.9683 121.662 -22.1036 0.075 1858 -1860 2 -57.3547 124.639 -22.8031 0.075 1859 -1861 2 -57.2969 127.691 -23.4514 0.075 1860 -1862 2 -58.042 130.693 -23.8254 0.075 1861 -1863 2 -58.894 133.59 -23.1333 0.075 1862 -1864 2 -59.9925 136.377 -22.2669 0.075 1863 -1865 2 -61.2453 139.201 -22.0803 0.075 1864 -1866 2 -62.5313 141.929 -22.7511 0.075 1865 -1867 2 -63.7887 144.407 -24.1255 0.075 1866 -1868 2 -64.6434 146.583 -26.1264 0.075 1867 -1869 2 -65.5232 147.972 -28.6549 0.075 1868 -1870 2 -67.0384 148.524 -31.2998 0.075 1869 -1871 2 -68.8041 149.075 -33.7927 0.075 1870 -1872 2 -70.1264 150.316 -36.2464 0.075 1871 -1873 2 -70.9744 152.575 -38.1659 0.075 1872 -1874 2 -71.9965 155.279 -39.3264 0.075 1873 -1875 2 -72.6433 158.001 -40.7074 0.075 1874 -1876 2 -73.2137 160.804 -41.9539 0.075 1875 -1877 2 -17.653 -28.6013 1.20636 0.075 1751 -1878 2 -18.1572 -25.3081 0.828169 0.075 1877 -1879 2 -17.9295 -21.9842 0.516282 0.075 1878 -1880 2 -17.4008 -18.6784 0.408893 0.075 1879 -1881 2 -17.0858 -15.3699 0.72432 0.075 1880 -1882 2 -16.4936 -12.1037 0.928638 0.075 1881 -1883 2 -15.433 -8.94477 0.709236 0.075 1882 -1884 2 -14.5968 -5.78857 0.222142 0.075 1883 -1885 2 -14.7159 -2.49886 -0.33852 0.075 1884 -1886 2 -15.305 0.727574 -1.01444 0.075 1885 -1887 2 -15.4506 3.98279 -1.80471 0.075 1886 -1888 2 -17.9925 4.89143 -3.36505 0.075 1887 -1889 2 -21.05 4.75774 -4.09091 0.075 1888 -1890 2 -23.6062 5.11283 -5.89084 0.075 1889 -1891 2 -26.031 5.90923 -7.72546 0.075 1890 -1892 2 -28.4431 7.20221 -9.25634 0.075 1891 -1893 2 -30.9653 8.87683 -9.95117 0.075 1892 -1894 2 -33.3412 10.8207 -9.60945 0.075 1893 -1895 2 -35.3404 12.5765 -10.5137 0.075 1894 -1896 2 -37.094 14.0282 -12.6806 0.075 1895 -1897 2 -39.0544 15.5379 -14.5727 0.075 1896 -1898 2 -41.2687 17.4482 -15.4392 0.075 1897 -1899 2 -43.5368 19.6254 -15.477 0.075 1898 -1900 2 -45.876 21.7268 -15.376 0.075 1899 -1901 2 -48.2147 23.8246 -15.2889 0.075 1900 -1902 2 -50.4027 26.0811 -15.3972 0.075 1901 -1903 2 -52.7638 28.1458 -15.6464 0.075 1902 -1904 2 -14.2025 5.97396 -1.07378 0.075 1887 -1905 2 -13.9162 6.71778 -4.03038 0.075 1904 -1906 2 -13.7 7.45536 -6.97505 0.075 1905 -1907 2 -13.2814 9.32385 -9.33076 0.075 1906 -1908 2 -12.7223 12.1387 -10.4008 0.075 1907 -1909 2 -11.6508 14.9644 -10.8687 0.075 1908 -1910 2 -10.5738 17.7926 -11.3405 0.075 1909 -1911 2 -9.8455 20.6966 -11.9447 0.075 1910 -1912 2 -9.03058 23.5498 -12.6793 0.075 1911 -1913 2 -7.88769 26.2578 -13.5116 0.075 1912 -1914 2 -6.8908 28.7546 -13.236 0.075 1913 -1915 2 -6.27954 31.0736 -11.3367 0.075 1914 -1916 2 -5.3134 33.4419 -9.75169 0.075 1915 -1917 2 -3.87829 36.0666 -9.2114 0.075 1916 -1918 2 -2.4406 38.7213 -9.72646 0.075 1917 -1919 2 -1.54089 41.5508 -10.4785 0.075 1918 -1920 2 -1.20401 44.5607 -10.2463 0.075 1919 -1921 2 -1.19036 46.9091 -8.43486 0.075 1920 -1922 2 -0.931807 48.832 -6.0821 0.075 1921 -1923 2 -0.56078 50.6519 -3.64664 0.075 1922 -1924 2 0.0315716 53.0017 -2.11254 0.075 1923 -1925 2 0.892569 55.8961 -1.84464 0.075 1924 -1926 2 2.05784 58.6847 -2.11257 0.075 1925 -1927 2 3.89055 61.1153 -2.35897 0.075 1926 -1928 2 6.06137 63.2746 -2.42487 0.075 1927 -1929 2 8.14155 65.5188 -2.55601 0.075 1928 -1930 2 10.0476 67.7485 -3.42488 0.075 1929 -1931 2 12.1827 69.7267 -4.36732 0.075 1930 -1932 2 14.5728 71.5743 -4.63687 0.075 1931 -1933 2 16.6192 73.6854 -4.03509 0.075 1932 -1934 2 18.218 76.1404 -3.18511 0.075 1933 -1935 2 19.976 78.537 -2.84679 0.075 1934 -1936 2 22.2864 80.4999 -3.15247 0.075 1935 -1937 2 24.799 82.1128 -3.78794 0.075 1936 -1938 2 27.1922 83.6546 -4.89353 0.075 1937 -1939 2 29.1535 85.5952 -6.2232 0.075 1938 -1940 2 31.1377 87.6318 -7.36166 0.075 1939 -1941 2 33.12 89.7518 -8.33764 0.075 1940 -1942 2 35.0249 91.9965 -9.17715 0.075 1941 -1943 2 36.5566 94.3838 -10.2821 0.075 1942 -1944 2 37.7436 96.7406 -11.809 0.075 1943 -1945 2 38.9123 98.9954 -13.5155 0.075 1944 -1946 2 40.2483 101.19 -15.1816 0.075 1945 -1947 2 41.5542 103.342 -16.9163 0.075 1946 -1948 2 42.5129 105.512 -18.8399 0.075 1947 -1949 2 42.9388 108.033 -20.5261 0.075 1948 -1950 2 -5.43577 -60.5531 5.58272 0.075 1683 -1951 2 -2.55715 -60.1675 7.47089 0.075 1950 -1952 2 0.447784 -60.065 9.28825 0.075 1951 -1953 2 3.24257 -60.2208 11.4411 0.075 1952 -1954 2 44.1985 -163.755 22.9818 0.075 1440 -1955 2 43.4853 -166.844 24.2689 0.075 1954 -1956 2 44.0999 -169.963 25.5429 0.075 1955 -1957 2 45.3333 -172.395 27.6429 0.075 1956 -1958 2 46.8624 -174.707 29.6755 0.075 1957 -1959 2 48.7383 -177.304 30.9141 0.075 1958 -1960 2 51.0018 -179.891 31.0928 0.075 1959 -1961 2 53.307 -182.442 31.2615 0.075 1960 -1962 2 53.8777 -185.564 31.7104 0.075 1961 -1963 2 54.1191 -188.726 32.1746 0.075 1962 -1964 2 54.1062 -190.625 34.7013 0.075 1963 -1965 2 54.0486 -192.288 37.4401 0.075 1964 -1966 2 54.8042 -194.302 39.7787 0.075 1965 -1967 2 55.892 -196.77 41.4718 0.075 1966 -1968 2 56.9545 -198.875 43.594 0.075 1967 -1969 2 57.9839 -200.592 46.0911 0.075 1968 -1970 2 58.8925 -202.476 48.5064 0.075 1969 -1971 2 59.4777 -204.91 50.4386 0.075 1970 -1972 2 59.672 -207.854 51.5413 0.075 1971 -1973 2 59.9286 -210.897 52.4158 0.075 1972 -1974 2 60.5839 -213.508 54.0353 0.075 1973 -1975 2 61.2426 -215.617 56.3474 0.075 1974 -1976 2 61.4918 -217.626 58.8213 0.075 1975 -1977 2 61.2545 -219.567 61.3535 0.075 1976 -1978 2 60.637 -221.428 63.8811 0.075 1977 -1979 2 60.2359 -222.789 66.7399 0.075 1978 -1980 2 60.2356 -223.167 69.923 0.075 1979 -1981 2 56.3418 -182.899 31.1235 0.075 1961 -1982 2 58.9199 -184.583 31.3624 0.075 1981 -1983 2 61.1325 -185.687 33.192 0.075 1982 -1984 2 63.1047 -186.665 35.3542 0.075 1983 -1985 2 64.8432 -188.811 36.4555 0.075 1984 -1986 2 66.5106 -191.363 36.8909 0.075 1985 -1987 2 68.2534 -193.894 37.1876 0.075 1986 -1988 2 69.9673 -196.424 37.6148 0.075 1987 -1989 2 71.6519 -198.891 38.3554 0.075 1988 -1990 2 73.4156 -201.147 39.4928 0.075 1989 -1991 2 74.9867 -203.317 40.961 0.075 1990 -1992 2 75.8379 -205.743 42.6725 0.075 1991 -1993 2 76.6125 -208.178 44.4056 0.075 1992 -1994 2 77.3229 -210.384 46.4472 0.075 1993 -1995 2 78.4177 -212.511 48.4001 0.075 1994 -1996 2 80.0297 -214.841 49.5798 0.075 1995 -1997 2 81.9957 -217.152 50.0365 0.075 1996 -1998 2 84.1815 -219.3 50.3965 0.075 1997 -1999 2 86.4649 -221.302 50.9413 0.075 1998 -2000 2 88.8517 -223.06 51.764 0.075 1999 -2001 2 91.2805 -224.677 52.7756 0.075 2000 -2002 2 93.5959 -226.44 53.8004 0.075 2001 -2003 2 95.7502 -228.45 54.6713 0.075 2002 -2004 2 97.8674 -230.617 54.9783 0.075 2003 -2005 2 100.789 -231.616 54.9565 0.075 2004 -2006 2 103.843 -232.012 55.1893 0.075 2005 -2007 2 106.837 -231.76 55.8664 0.075 2006 -2008 2 109.772 -231.531 56.7965 0.075 2007 -2009 2 112.721 -231.898 57.5558 0.075 2008 -2010 2 115.672 -232.448 58.2856 0.075 2009 -2011 2 118.224 -233.299 59.6382 0.075 2010 -2012 2 120.435 -234.423 61.4777 0.075 2011 -2013 2 122.678 -235.44 63.3296 0.075 2012 -2014 2 125.109 -236.161 65.0825 0.075 2013 -2015 2 127.766 -236.73 66.5382 0.075 2014 -2016 2 130.59 -237.14 67.6999 0.075 2015 -2017 2 132.985 -237.2 69.5541 0.075 2016 -2018 2 133.889 -236.877 72.4898 0.075 2017 -2019 2 56.129 -163.145 26.1825 0.075 1437 -2020 2 58.796 -164.934 26.7351 0.075 2019 -2021 2 61.7894 -166.037 27.3145 0.075 2020 -2022 2 64.7754 -166.117 28.4644 0.075 2021 -2023 2 67.8822 -166.372 28.6796 0.075 2022 -2024 2 71.1087 -166.628 28.4287 0.075 2023 -2025 2 74.3615 -166.451 28.4209 0.075 2024 -2026 2 77.5719 -166.045 28.6753 0.075 2025 -2027 2 80.6756 -165.902 29.6763 0.075 2026 -2028 2 80.964 -167.592 32.2872 0.075 2027 -2029 2 81.3991 -169.505 34.7178 0.075 2028 -2030 2 81.9848 -171.572 36.9833 0.075 2029 -2031 2 83.1546 -173.795 38.8018 0.075 2030 -2032 2 84.6004 -176.046 40.4108 0.075 2031 -2033 2 85.925 -178.34 42.058 0.075 2032 -2034 2 87.0799 -180.659 43.8021 0.075 2033 -2035 2 87.9726 -182.493 46.0363 0.075 2034 -2036 2 88.522 -183.602 48.8972 0.075 2035 -2037 2 89.0086 -184.657 51.7795 0.075 2036 -2038 2 89.7071 -186.179 54.4133 0.075 2037 -2039 2 90.238 -187.822 57.0004 0.075 2038 -2040 2 90.4257 -188.788 59.9649 0.075 2039 -2041 2 83.7694 -164.777 27.1264 0.075 2027 -2042 2 86.6057 -163.108 24.571 0.075 2041 -2043 2 89.2847 -161.57 21.7739 0.075 2042 -2044 2 88.9472 -166.045 21.9034 0.075 2043 -2045 2 88.7215 -170.265 23.2449 0.075 2044 -2046 2 89.0204 -172.448 27.1632 0.075 2045 -2047 2 91.5079 -159.29 20.8672 0.075 2043 -2048 2 93.3064 -157.129 19.1163 0.075 2047 -2049 2 96.1725 -155.559 19.7035 0.075 2048 -2050 2 99.1525 -154.679 20.8722 0.075 2049 -2051 2 102.01 -153.783 22.3035 0.075 2050 -2052 2 104.699 -153.579 24.2196 0.075 2051 -2053 2 107.41 -153.408 23.3068 0.075 2052 -2054 2 108.106 -152.395 20.2476 0.075 2053 -2055 2 110.024 -151.43 17.8351 0.075 2054 -2056 2 113.201 -150.707 17.2716 0.075 2055 -2057 2 116.181 -149.304 17.5219 0.075 2056 -2058 2 118.466 -147.988 19.538 0.075 2057 -2059 2 121.105 -147.089 21.3408 0.075 2058 -2060 2 123.482 -148.698 22.3961 0.075 2059 -2061 2 125.076 -151.079 23.4984 0.075 2060 -2062 2 126.222 -153.894 23.888 0.075 2061 -2063 2 127.339 -156.726 24.2813 0.075 2062 -2064 2 128.57 -159.45 24.9568 0.075 2063 -2065 2 129.575 -161.288 23.3671 0.075 2064 -2066 2 130.475 -162.788 20.8442 0.075 2065 -2067 2 131.359 -164.607 18.5683 0.075 2066 -2068 2 132.216 -167.1 17.2159 0.075 2067 -2069 2 133.291 -169.839 16.4287 0.075 2068 -2070 2 134.719 -172.204 15.1592 0.075 2069 -2071 2 136.12 -174.252 13.352 0.075 2070 -2072 2 137.469 -176.337 11.5536 0.075 2071 -2073 2 138.932 -178.609 10.0989 0.075 2072 -2074 2 140.599 -180.887 8.89161 0.075 2073 -2075 2 142.37 -183.116 7.74354 0.075 2074 -2076 2 144.082 -185.217 6.30999 0.075 2075 -2077 2 145.251 -187.772 5.17242 0.075 2076 -2078 2 146.285 -190.594 4.95927 0.075 2077 -2079 2 147.634 -193.317 5.27404 0.075 2078 -2080 2 149.815 -195.139 5.22048 0.075 2079 -2081 2 152.648 -196.068 4.51279 0.075 2080 -2082 2 155.436 -197.06 3.76897 0.075 2081 -2083 2 158.243 -198.202 3.78746 0.075 2082 -2084 2 160.901 -199.372 4.77009 0.075 2083 -2085 2 163.677 -200.577 5.2839 0.075 2084 -2086 2 123.623 -144.647 20.8809 0.075 2059 -2087 2 126.701 -142.938 20.4658 0.075 2086 -2088 2 129.348 -140.615 20.0018 0.075 2087 -2089 2 132.209 -138.555 19.5715 0.075 2088 -2090 2 135.565 -137.711 18.7697 0.075 2089 -2091 2 138.11 -135.371 17.9507 0.075 2090 -2092 2 139.036 -133.99 20.5235 0.075 2091 -2093 2 140.443 -132.038 22.4111 0.075 2092 -2094 2 141.951 -130.134 24.2774 0.075 2093 -2095 2 143.777 -128.562 26.1487 0.075 2094 -2096 2 145.314 -126.826 28.1089 0.075 2095 -2097 2 146.174 -124.754 30.1464 0.075 2096 -2098 2 146.54 -122.446 32.1184 0.075 2097 -2099 2 146.824 -119.924 33.8132 0.075 2098 -2100 2 147.137 -117.156 35.0856 0.075 2099 -2101 2 147.282 -114.319 36.2319 0.075 2100 -2102 2 148.117 -111.757 37.688 0.075 2101 -2103 2 149.65 -110.024 39.6578 0.075 2102 -2104 2 151.127 -108.365 41.7657 0.075 2103 -2105 2 152.157 -106.84 44.1873 0.075 2104 -2106 2 153.076 -105.159 46.5613 0.075 2105 -2107 2 154.239 -102.955 48.1794 0.075 2106 -2108 2 155.735 -100.58 49.3384 0.075 2107 -2109 2 157.314 -98.3396 50.6019 0.075 2108 -2110 2 158.266 -95.5248 51.1153 0.075 2109 -2111 2 158.368 -92.4807 51.2367 0.075 2110 -2112 2 158.306 -89.586 52.2232 0.075 2111 -2113 2 158.37 -87.464 54.4315 0.075 2112 -2114 2 158.273 -86.2989 52.0785 0.075 2113 -2115 2 158.147 -85.5629 49.1079 0.075 2114 -2116 2 157.91 -84.5366 46.2346 0.075 2115 -2117 2 157.367 -82.7861 43.8287 0.075 2116 -2118 2 156.578 -80.4637 42.0579 0.075 2117 -2119 2 155.668 -77.8014 40.8975 0.075 2118 -2120 2 154.735 -75.0092 40.0512 0.075 2119 -2121 2 153.791 -72.2749 39.0716 0.075 2120 -2122 2 152.764 -69.747 37.6868 0.075 2121 -2123 2 151.622 -67.4447 36.0199 0.075 2122 -2124 2 151.917 -64.4 37.6812 0.075 2123 -2125 2 150.413 -61.6804 35.8013 0.075 2124 -2126 2 149.232 -59.0952 33.5434 0.075 2125 -2127 2 148.301 -56.918 30.7853 0.075 2126 -2128 2 147.289 -54.5713 28.2001 0.075 2127 -2129 2 146.561 -52.1607 25.5778 0.075 2128 -2130 2 145.906 -49.6143 23.067 0.075 2129 -2131 2 151.691 -67.5308 39.2031 0.075 2123 -2132 2 151.759 -67.617 42.3864 0.075 2131 -2133 2 151.829 -67.703 45.5696 0.075 2132 -2134 2 150.131 -69.8591 46.7938 0.075 2133 -2135 2 148.172 -71.4476 48.6783 0.075 2134 -2136 2 146.649 -72.4112 51.2753 0.075 2135 -2137 2 144.974 -73.2799 53.8369 0.075 2136 -2138 2 142.591 -74.4041 55.488 0.075 2137 -2139 2 139.835 -75.737 56.2903 0.075 2138 -2140 2 137.593 -76.9587 57.9394 0.075 2139 -2141 2 136.322 -77.9343 60.6266 0.075 2140 -2142 2 135.702 -79.0288 63.5377 0.075 2141 -2143 2 135.716 -80.7361 66.1489 0.075 2142 -2144 2 136.589 -83.1612 67.7186 0.075 2143 -2145 2 137.93 -85.7624 68.8539 0.075 2144 -2146 2 138.834 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2532 -2534 2 71.9841 71.7264 146.811 0.075 2533 -2535 2 71.4048 74.5419 147.893 0.075 2534 -2536 2 70.3572 77.3771 148.495 0.075 2535 -2537 2 70.7056 80.0452 150.09 0.075 2536 -2538 2 70.9027 82.7447 151.658 0.075 2537 -2539 2 71.1074 85.4875 153.148 0.075 2538 -2540 2 71.7306 87.8537 155.079 0.075 2539 -2541 2 71.5311 90.3127 156.954 0.075 2540 -2542 2 71.1277 93.1057 158.256 0.075 2541 -2543 2 71.3404 95.9372 159.497 0.075 2542 -2544 2 71.7506 98.7371 160.829 0.075 2543 -2545 2 72.0231 101.666 161.834 0.075 2544 -2546 2 72.0836 104.748 162.222 0.075 2545 -2547 2 71.7621 107.839 162.104 0.075 2546 -2548 2 70.9083 110.815 161.784 0.075 2547 -2549 2 69.6836 113.682 161.63 0.075 2548 -2550 2 68.4936 116.553 161.904 0.075 2549 -2551 2 67.1605 119.299 162.588 0.075 2550 -2552 2 65.8268 122.035 163.294 0.075 2551 -2553 2 64.7612 124.968 163.518 0.075 2552 -2554 2 63.9408 127.963 163.891 0.075 2553 -2555 2 63.198 130.903 164.651 0.075 2554 -2556 2 62.5982 133.955 164.735 0.075 2555 -2557 2 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149.872 0.075 2579 -2581 2 80.9484 181.619 147.927 0.075 2580 -2582 2 83.6181 182.903 148.664 0.075 2581 -2583 2 85.6907 184.206 150.782 0.075 2582 -2584 2 87.8604 185.649 152.631 0.075 2583 -2585 2 90.3391 187.573 153.405 0.075 2584 -2586 2 92.9013 189.548 153.529 0.075 2585 -2587 2 51.9337 170.667 167.822 0.075 2568 -2588 2 50.563 173.402 167.244 0.075 2587 -2589 2 49.5483 176.329 166.953 0.075 2588 -2590 2 48.3103 179.087 167.6 0.075 2589 -2591 2 47.3904 181.945 168.405 0.075 2590 -2592 2 46.3946 184.818 169.062 0.075 2591 -2593 2 45.1061 187.61 169.506 0.075 2592 -2594 2 43.8706 190.311 170.345 0.075 2593 -2595 2 43.1223 192.941 171.769 0.075 2594 -2596 2 42.8577 195.67 173.209 0.075 2595 -2597 2 42.4807 198.513 174.366 0.075 2596 -2598 2 41.5028 201.238 175.488 0.075 2597 -2599 2 40.1833 203.853 176.527 0.075 2598 -2600 2 38.7336 206.526 177.089 0.075 2599 -2601 2 37.3163 209.29 176.944 0.075 2600 -2602 2 36.2538 212.214 176.852 0.075 2601 -2603 2 35.2076 215.13 177.166 0.075 2602 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159.009 0.075 2626 -2628 2 47.9364 79.1649 159.714 0.075 2627 -2629 2 45.0959 79.9335 160.465 0.075 2628 -2630 2 42.1811 80.2853 161.267 0.075 2629 -2631 2 39.3346 80.6786 162.26 0.075 2630 -2632 2 37.1453 81.6455 164.141 0.075 2631 -2633 2 35.0854 82.9325 165.973 0.075 2632 -2634 2 32.6324 84.3222 167.036 0.075 2633 -2635 2 29.9149 85.5178 167.663 0.075 2634 -2636 2 27.0816 86.5708 167.977 0.075 2635 -2637 2 24.29 87.5196 168.576 0.075 2636 -2638 2 21.9139 88.3494 170.142 0.075 2637 -2639 2 19.9904 89.124 172.371 0.075 2638 -2640 2 17.9766 89.8228 174.527 0.075 2639 -2641 2 15.5908 90.2026 176.375 0.075 2640 -2642 2 13.0279 90.3873 178.008 0.075 2641 -2643 2 10.2035 89.9918 179.071 0.075 2642 -2644 2 7.36473 89.1357 179.743 0.075 2643 -2645 2 4.52846 88.2633 180.422 0.075 2644 -2646 2 2.23579 87.2849 182.032 0.075 2645 -2647 2 0.772568 86.2758 184.455 0.075 2646 -2648 2 -0.526662 85.2329 187.001 0.075 2647 -2649 2 -2.22071 84.1027 189.217 0.075 2648 -2650 2 -4.49567 82.9223 190.77 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0.075 3573 -3575 2 31.5021 38.2071 -10.9192 0.0358442 3553 -3576 2 28.2371 37.6607 -10.5926 0.035 3575 -3577 2 25.1855 36.3868 -10.2335 0.035 3576 -3578 2 22.0908 35.1758 -9.8811 0.035 3577 -3579 2 18.9525 34.0839 -9.48223 0.035 3578 -3580 2 15.9249 32.8992 -8.79055 0.035 3579 -3581 2 13.3013 31.6257 -7.30549 0.035 3580 -3582 2 11.4978 30.8256 -4.60136 0.035 3581 -3583 2 53.3685 18.2804 9.88186 0.075 3541 -3584 2 49.4695 19.2315 10.168 0.075 3583 -3585 2 45.6434 20.4669 10.3411 0.075 3584 -3586 2 41.7843 21.6038 10.4483 0.075 3585 -3587 2 60.4477 16.5877 6.09272 0.075 3540 -3588 2 60.8864 19.1415 4.01975 0.075 3587 -3589 2 62.1555 21.9346 2.87767 0.075 3588 -3590 2 63.7077 24.6996 3.2117 0.075 3589 -3591 2 65.2827 27.4248 4.26162 0.075 3590 -3592 2 66.9477 30.1386 5.00954 0.075 3591 -3593 2 69.0006 32.7184 4.94315 0.075 3592 -3594 2 71.6086 34.7506 4.65147 0.075 3593 -3595 2 74.3986 32.2306 4.78481 0.075 3594 -3596 2 72.8478 29.8959 3.78095 0.075 3595 -3597 2 70.1748 28.4217 3.82231 0.075 3596 -3598 2 67.4915 27.0096 3.46279 0.075 3597 -3599 2 65.4207 25.2019 2.18528 0.075 3598 -3600 2 63.6383 23.0518 0.966741 0.075 3599 -3601 2 61.702 20.7856 0.669178 0.075 3600 -3602 2 59.8609 18.362 0.813098 0.075 3601 -3603 2 58.3937 15.7083 0.795327 0.075 3602 -3604 2 57.3285 12.9147 0.305486 0.075 3603 -3605 2 56.5448 10.0737 -0.41725 0.075 3604 -3606 2 55.983 7.08631 -0.563071 0.075 3605 -3607 2 55.9739 4.03353 -0.519357 0.075 3606 -3608 2 56.1514 0.985375 -0.534539 0.075 3607 -3609 2 56.0316 -2.05909 -0.708028 0.075 3608 -3610 2 55.7771 -5.01375 -1.39 0.075 3609 -3611 2 55.8384 -7.76095 -2.65794 0.075 3610 -3612 2 56.0967 -10.4002 -4.17036 0.075 3611 -3613 2 56.3567 -12.9428 -5.83302 0.075 3612 -3614 2 56.4909 -15.3289 -7.72404 0.075 3613 -3615 2 56.3346 -17.6499 -9.67977 0.075 3614 -3616 2 55.7722 -20.2214 -11.1341 0.075 3615 -3617 2 55.192 -23.2042 -11.4026 0.075 3616 -3618 2 55.3088 -26.2152 -11.8962 0.075 3617 -3619 2 55.8137 -29.1789 -12.4173 0.075 3618 -3620 2 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4.63814 0.075 3639 -3643 2 86.2302 31.1351 4.14424 0.075 3642 -3644 2 87.8226 34.1013 3.30391 0.075 3643 -3645 2 89.719 36.8414 2.20083 0.075 3644 -3646 2 86.5539 25.2269 4.77279 0.075 3642 -3647 2 89.4363 24.1599 4.98327 0.075 3646 -3648 2 91.8115 23.3158 6.70575 0.075 3647 -3649 2 94.3395 22.4181 8.21085 0.075 3648 -3650 2 97.1446 22.0427 9.34789 0.075 3649 -3651 2 100.066 21.7092 10.2464 0.075 3650 -3652 2 102.962 20.9298 10.7482 0.075 3651 -3653 2 105.872 20.0211 10.9195 0.075 3652 -3654 2 108.912 19.7288 11.3029 0.075 3653 -3655 2 111.928 19.5783 11.8868 0.075 3654 -3656 2 114.8 18.7069 12.5812 0.075 3655 -3657 2 117.383 17.1439 13.1554 0.075 3656 -3658 2 119.926 15.4414 13.4886 0.075 3657 -3659 2 122.534 13.8299 13.7735 0.075 3658 -3660 2 125.003 12.0188 13.9608 0.075 3659 -3661 2 127.441 10.1686 13.8577 0.075 3660 -3662 2 129.938 8.68746 14.2135 0.075 3661 -3663 2 132.292 7.45118 15.7699 0.075 3662 -3664 2 134.686 6.25139 17.2816 0.075 3663 -3665 2 137.38 5.32761 18.4562 0.075 3664 -3666 2 137.057 3.17907 21.8349 0.075 3665 -3667 2 136.476 -0.0251783 24.2061 0.075 3666 -3668 2 136.353 -3.5919 26.0791 0.075 3667 -3669 2 135.203 -8.8419 27.3991 0.075 3668 -3670 2 137.425 -5.36192 23.4569 0.075 3668 -3671 2 139.287 -8.06539 23.3594 0.075 3670 -3672 2 141.376 -10.667 23.4744 0.075 3671 -3673 2 143.388 -13.331 23.5323 0.075 3672 -3674 2 144.733 -16.1498 22.766 0.075 3673 -3675 2 145.589 -19.1304 21.5464 0.075 3674 -3676 2 146.477 -22.2173 21.0714 0.075 3675 -3677 2 147.384 -25.3017 21.9472 0.075 3676 -3678 2 147.912 -28.3219 23.2733 0.075 3677 -3679 2 139.4 5.13995 20.7455 0.075 3665 -3680 2 142.286 4.74531 21.6895 0.075 3679 -3681 2 145.125 4.42865 22.7895 0.075 3680 -3682 2 147.064 4.23538 25.08 0.075 3681 -3683 2 148.932 4.0118 27.4952 0.075 3682 -3684 2 151.48 3.4018 28.8158 0.075 3683 -3685 2 154.297 2.34168 29.1126 0.075 3684 -3686 2 156.984 0.901503 29.2311 0.075 3685 -3687 2 159.627 -0.582537 29.6053 0.075 3686 -3688 2 162.437 -1.39499 30.1963 0.075 3687 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3710 -3712 2 222.317 -20.9456 51.5265 0.075 3711 -3713 2 225.331 -21.3398 51.1797 0.075 3712 -3714 2 228.366 -21.6326 50.9357 0.075 3713 -3715 2 231.399 -21.4683 50.7336 0.075 3714 -3716 2 234.427 -21.0766 50.5252 0.075 3715 -3717 2 237.473 -20.9075 50.3239 0.075 3716 -3718 2 240.502 -20.9046 49.9739 0.075 3717 -3719 2 243.455 -20.8361 49.1809 0.075 3718 -3720 2 246.418 -21.1321 48.565 0.075 3719 -3721 2 249.34 -21.9976 48.8368 0.075 3720 -3722 2 252.041 -23.3246 49.3994 0.075 3721 -3723 2 254.801 -24.0437 48.3414 0.075 3722 -3724 2 257.517 -24.2805 46.956 0.075 3723 -3725 2 260.52 -24.374 46.6379 0.075 3724 -3726 2 262.916 -24.807 48.22 0.075 3725 -3727 2 264.188 -25.5859 50.8274 0.075 3726 -3728 2 265.196 -26.4338 53.5915 0.075 3727 -3729 2 266.359 -27.729 56.0695 0.075 3728 -3730 2 267.771 -29.824 57.6486 0.075 3729 -3731 2 269.235 -32.2185 58.8685 0.075 3730 -3732 2 270.684 -34.5595 60.2069 0.075 3731 -3733 2 272.194 -36.6185 61.8285 0.075 3732 -3734 2 273.738 -38.2129 63.9369 0.075 3733 -3735 2 275.233 -39.8313 66.062 0.075 3734 -3736 2 276.732 -41.6486 67.9913 0.075 3735 -3737 2 278.09 -44.1429 69.136 0.075 3736 -3738 2 72.9058 38.5174 3.5016 0.075 3594 -3739 2 73.6968 42.2497 1.86275 0.075 3738 -3740 2 72.9754 45.0919 2.56447 0.075 3739 -3741 2 73.1663 48.0408 3.17235 0.075 3740 -3742 2 73.0048 50.9982 2.71992 0.075 3741 -3743 2 73.3899 53.5923 1.30407 0.075 3742 -3744 2 74.4143 56.1144 0.0503985 0.075 3743 -3745 2 75.0275 58.921 -0.694953 0.075 3744 -3746 2 75.7366 61.7682 -1.30033 0.075 3745 -3747 2 76.8538 64.4967 -1.92574 0.075 3746 -3748 2 77.9555 67.2481 -2.44019 0.075 3747 -3749 2 78.8105 70.1152 -2.82808 0.075 3748 -3750 2 79.6547 72.9435 -3.36459 0.075 3749 -3751 2 80.3823 75.6005 -4.58945 0.075 3750 -3752 2 80.7024 78.3108 -5.87529 0.075 3751 -3753 2 80.9018 81.1486 -6.87763 0.075 3752 -3754 2 81.4177 83.9505 -7.86177 0.075 3753 -3755 2 81.8141 86.8295 -8.65816 0.075 3754 -3756 2 82.1091 89.7325 -9.42331 0.075 3755 -3757 2 82.0484 92.6366 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141.327 -74.2169 -16.0594 0.075 5854 -5856 2 143.398 -74.1047 -18.5405 0.075 5855 -5857 2 145.109 -73.7638 -21.2773 0.075 5856 -5858 2 146.901 -73.6209 -23.9795 0.075 5857 -5859 2 148.424 -73.9639 -26.801 0.075 5858 -5860 2 149.367 -74.7068 -29.796 0.075 5859 -5861 2 150.126 -75.3617 -32.8722 0.075 5860 -5862 2 151.661 -75.6131 -35.5395 0.075 5861 -5863 2 154.483 -75.4404 -36.9492 0.075 5862 -5864 2 157.626 -74.7577 -37.415 0.075 5863 -5865 2 160.676 -74.3449 -38.1826 0.075 5864 -5866 2 163.313 -75.5788 -39.5917 0.075 5865 -5867 2 165.418 -77.8157 -40.6563 0.075 5866 -5868 2 45.33 -50.4764 19.4729 0.075 5679 -5869 2 45.4046 -47.6397 17.8741 0.075 5868 -5870 2 44.7934 -44.5952 16.8956 0.075 5869 -5871 2 43.7165 -41.5924 16.2607 0.075 5870 -5872 2 43.0306 -38.6758 15.028 0.075 5871 -5873 2 42.7161 -35.9471 13.2996 0.075 5872 -5874 2 42.2896 -33.1156 11.7701 0.075 5873 -5875 2 41.7149 -30.3981 10.0907 0.075 5874 -5876 2 41.1203 -27.6405 8.4853 0.075 5875 -5877 2 40.5706 -24.623 7.47191 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5889 -5901 2 22.7927 16.6825 0.952318 0.075 5900 -5902 2 21.3375 19.5863 0.492765 0.075 5901 -5903 2 20.1889 22.6366 0.157934 0.075 5902 -5904 2 19.3604 25.7891 -0.17773 0.075 5903 -5905 2 18.5365 28.9459 -0.515974 0.075 5904 -5906 2 17.374 31.9854 -0.822159 0.075 5905 -5907 2 16.0785 34.9822 -1.1151 0.075 5906 -5908 2 14.8607 38.0162 -1.36341 0.075 5907 -5909 2 13.4424 40.9676 -1.45911 0.075 5908 -5910 2 11.8365 43.8248 -1.47305 0.075 5909 -5911 2 10.4595 46.7902 -1.71129 0.075 5910 -5912 2 9.23461 49.7697 -2.33241 0.075 5911 -5913 2 5.00388 49.6973 0.224846 0.075 5912 -5914 2 0.445036 49.7553 2.14619 0.075 5913 -5915 2 8.92055 52.6559 -1.58069 0.075 5912 -5916 2 9.72227 55.073 0.0776329 0.075 5915 -5917 2 10.9187 57.3988 1.62392 0.075 5916 -5918 2 11.9827 59.8986 2.98085 0.075 5917 -5919 2 12.9757 62.645 3.74816 0.075 5918 -5920 2 13.7888 65.5342 3.72135 0.075 5919 -5921 2 14.2463 68.5166 3.50376 0.075 5920 -5922 2 14.213 71.5393 3.42275 0.075 5921 -5923 2 13.7881 74.5449 3.30589 0.075 5922 -5924 2 13.3108 77.5387 3.10941 0.075 5923 -5925 2 12.9943 80.5567 3.02065 0.075 5924 -5926 2 13.0393 83.5875 3.23905 0.075 5925 -5927 2 13.0959 86.4997 4.09892 0.075 5926 -5928 2 13.0001 89.4498 4.81644 0.075 5927 -5929 2 12.7576 92.4576 4.93345 0.075 5928 -5930 2 12.323 95.4527 4.71036 0.075 5929 -5931 2 11.8785 98.4316 4.32077 0.075 5930 -5932 2 11.5854 101.352 3.60386 0.075 5931 -5933 2 11.6597 103.64 3.81349 0.075 5932 -5934 2 12.2189 104.867 6.53709 0.075 5933 -5935 2 12.7532 106.251 9.11466 0.075 5934 -5936 2 13.0789 108.953 10.4655 0.075 5935 -5937 2 13.3481 111.794 11.5103 0.075 5936 -5938 2 13.6174 114.635 12.5551 0.075 5937 -5939 2 13.6835 117.605 13.1435 0.075 5938 -5940 2 13.9238 120.559 13.7907 0.075 5939 -5941 2 14.4521 123.363 14.7928 0.075 5940 -5942 2 15.132 126.047 16.0434 0.075 5941 -5943 2 15.6347 128.622 17.5359 0.075 5942 -5944 2 15.7949 131.079 19.3175 0.075 5943 -5945 2 15.9169 133.565 21.0477 0.075 5944 -5946 2 15.99 136.316 22.3317 0.075 5945 -5947 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0.075 1790 -1792 2 170.632 -88.0613 2.86145 0.075 1791 -1793 2 173.321 -86.7769 1.90306 0.075 1792 -1794 2 175.824 -84.9346 1.40013 0.075 1793 -1795 2 175.582 -83.8462 -1.77076 0.075 1794 -1796 2 174.637 -82.902 -4.85077 0.075 1795 -1797 2 173.304 -81.927 -7.77937 0.075 1796 -1798 2 172.029 -80.884 -10.7109 0.075 1797 -1799 2 170.555 -79.8777 -13.5544 0.075 1798 -1800 2 169.178 -78.2327 -16.1012 0.075 1799 -1801 2 169.125 -75.3157 -17.5915 0.075 1800 -1802 2 171.595 -72.9279 -19.5713 0.075 1801 -1803 2 172.105 -70.8904 -22.9237 0.075 1802 -1804 2 171.506 -68.7499 -26.2665 0.075 1803 -1805 2 171.797 -66.3202 -29.4246 0.075 1804 -1806 2 168.146 -74.5364 -20.451 0.075 1801 -1807 2 167.272 -73.1357 -23.0715 0.075 1806 -1808 2 166.514 -70.6995 -24.7457 0.075 1807 -1809 2 165.458 -67.8034 -25.2163 0.075 1808 -1810 2 164.964 -64.788 -25.6511 0.075 1809 -1811 2 165.429 -61.7474 -25.926 0.075 1810 -1812 2 166.05 -58.7208 -25.6467 0.075 1811 -1813 2 166.241 -55.6256 -25.3675 0.075 1812 -1814 2 165.98 -52.5655 -24.8583 0.075 1813 -1815 2 165.236 -49.558 -24.7363 0.075 1814 -1816 2 165.81 -46.5036 -24.6066 0.075 1815 -1817 2 165.493 -43.4524 -24.469 0.075 1816 -1818 2 164.806 -40.4741 -24.8758 0.075 1817 -1819 2 164.735 -37.3816 -25.2843 0.075 1818 -1820 2 164.602 -34.276 -25.297 0.075 1819 -1821 2 164.052 -32.096 -26.9167 0.075 1820 -1822 2 163.428 -31.0289 -29.7726 0.075 1821 -1823 2 180.444 -84.2646 4.6268 0.075 1794 -1824 2 184.085 -85.2015 2.6325 0.075 1823 -1825 2 188.298 -84.901 2.27353 0.075 1824 -1826 2 183.699 -83.0026 4.62074 0.075 1823 -1827 2 187.118 -82.9124 5.1716 0.075 1826 -1828 2 190.095 -81.6584 6.43397 0.075 1827 -1829 2 193.405 -80.7567 5.9776 0.075 1828 -1830 2 196.074 -79.2572 4.31413 0.075 1829 -1831 2 197.577 -77.0134 4.76679 0.075 1830 -1832 2 199.102 -75.7119 8.1806 0.075 1831 -1833 2 200.476 -72.5256 9.01603 0.075 1832 -1834 2 200.589 -68.581 9.23613 0.075 1833 -1835 2 200.818 -64.7581 9.67551 0.075 1834 -1836 2 201.667 -61.0104 9.25371 0.075 1835 -1837 2 202.008 -57.2341 9.78442 0.075 1836 -1838 2 202.677 -53.7908 11.4227 0.075 1837 -1839 2 201.432 -67.6699 13.1974 0.075 1834 -1840 2 202.33 -66.4338 14.1485 0.075 1839 -1841 2 203.072 -64.8231 10.3986 0.075 1840 -1842 2 198.434 -80.2681 1.31984 0.075 1830 -1843 2 199.533 -80.6926 -2.49236 0.075 1842 -1844 2 200.162 -80.6661 -6.32425 0.075 1843 -1845 2 202.087 -80.7804 -9.68167 0.075 1844 -1846 2 204.939 -81.9036 -9.73889 0.075 1845 -1847 2 207.225 -83.4391 -8.37952 0.075 1846 -1848 2 209.035 -85.3804 -6.92759 0.075 1847 -1849 2 210.83 -86.112 -4.58195 0.075 1848 -1850 2 212.824 -86.2801 -2.26527 0.075 1849 -1851 2 215.353 -87.2719 -1.27627 0.075 1850 -1852 2 217.905 -88.9431 -0.820958 0.075 1851 -1853 2 220.355 -90.4238 0.296838 0.075 1852 -1854 2 222.657 -92.0699 1.53549 0.075 1853 -1855 2 224.354 -94.5189 2.31247 0.075 1854 -1856 2 225.668 -96.8614 3.74766 0.075 1855 -1857 2 227.223 -98.4133 5.82633 0.075 1856 -1858 2 229.605 -99.9457 7.05545 0.075 1857 -1859 2 231.605 -101.233 8.84562 0.075 1858 -1860 2 233.214 -102.56 11.1152 0.075 1859 -1861 2 234.829 -104.753 12.3909 0.075 1860 -1862 2 236.222 -107.416 13.0938 0.075 1861 -1863 2 237.683 -109.828 14.3404 0.075 1862 -1864 2 201.662 -80.1483 -13.2022 0.075 1845 -1865 2 202.154 -79.9794 -16.7371 0.075 1864 -1866 2 202.343 -79.4918 -20.2916 0.075 1865 -1867 2 202.069 -82.7275 -19.9537 0.075 1866 -1868 2 202.869 -85.8701 -19.6009 0.075 1867 -1869 2 204.455 -88.6717 -19.2434 0.075 1868 -1870 2 206.7 -90.9455 -18.623 0.075 1869 -1871 2 208.235 -93.1834 -17.0634 0.075 1870 -1872 2 208.664 -95.7471 -15.1103 0.075 1871 -1873 2 208.935 -98.2641 -13.0537 0.075 1872 -1874 2 208.808 -100.378 -10.5824 0.075 1873 -1875 2 206.687 -102.474 -9.55521 0.075 1874 -1876 2 202.245 -76.6369 -21.6925 0.075 1866 -1877 2 203.094 -75.646 -24.5105 0.075 1876 -1878 2 205.62 -74.3004 -25.8321 0.075 1877 -1879 2 207.672 -73.6537 -27.7485 0.075 1878 -1880 2 208.953 -73.3074 -30.6013 0.075 1879 -1881 2 210.02 -71.7086 -33.04 0.075 1880 -1882 2 211.256 -69.1522 -34.3597 0.075 1881 -1883 2 213.058 -66.7551 -35.3923 0.075 1882 -1884 2 215.077 -65.2068 -37.2699 0.075 1883 -1885 2 32.5048 -114.036 21.3525 0.075 1171 -1886 2 31.0251 -112.222 19.1924 0.075 1885 -1887 2 29.2949 -110.288 17.3806 0.075 1886 -1888 2 27.0857 -108.388 16.1359 0.075 1887 -1889 2 24.7919 -106.628 14.8241 0.075 1888 -1890 2 22.5578 -105.313 13.0378 0.075 1889 -1891 2 19.9673 -103.764 12.3037 0.075 1890 -1892 2 17.0536 -102.664 12.8672 0.075 1891 -1893 2 14.0092 -101.919 13.414 0.075 1892 -1894 2 10.8982 -101.298 13.3596 0.075 1893 -1895 2 7.93097 -100.743 12.4507 0.075 1894 -1896 2 5.20114 -100.041 10.9755 0.075 1895 -1897 2 2.80696 -98.768 9.34591 0.075 1896 -1898 2 0.383407 -97.1782 8.04735 0.075 1897 -1899 2 -2.30918 -95.5247 7.85088 0.075 1898 -1900 2 -2.0855 -95.2131 4.44475 0.075 1899 -1901 2 -2.59558 -94.1139 1.26189 0.075 1900 -1902 2 -4.65791 -93.0563 -1.24427 0.075 1901 -1903 2 -6.84611 -92.2841 -3.76687 0.075 1902 -1904 2 -8.54835 -91.7191 -6.6873 0.075 1903 -1905 2 -5.10729 -94.8318 10.8101 0.075 1899 -1906 2 -6.76222 -95.7147 14.602 0.075 1905 -1907 2 -7.82621 -96.4153 18.6622 0.075 1906 -1908 2 37.6353 -108.429 28.0864 0.075 1170 -1909 2 41.1654 -109.986 28.8605 0.075 1908 -1910 2 43.6646 -108.564 31.3744 0.075 1909 -1911 2 45.4335 -105.783 33.6018 0.075 1910 -1912 2 47.0744 -102.047 33.3484 0.075 1911 -1913 2 50.591 -101.405 35.1672 0.075 1912 -1914 2 54.2293 -102.195 36.5959 0.075 1913 -1915 2 56.39 -104.948 38.4643 0.075 1914 -1916 2 60.1907 -105.324 38.9103 0.075 1915 -1917 2 63.8279 -106.517 39.2191 0.075 1916 -1918 2 66.4807 -107.275 40.5428 0.075 1917 -1919 2 68.8941 -108.94 41.304 0.075 1918 -1920 2 70.7382 -111.363 41.4896 0.075 1919 -1921 2 72.2883 -113.991 41.718 0.075 1920 -1922 2 74.0203 -116.498 41.9633 0.075 1921 -1923 2 75.7417 -119.001 42.298 0.075 1922 -1924 2 77.7085 -121.316 42.4163 0.075 1923 -1925 2 80.118 -123.176 42.2159 0.075 1924 -1926 2 82.6761 -124.847 42.2678 0.075 1925 -1927 2 84.6798 -127.041 42.9762 0.075 1926 -1928 2 86.3401 -129.55 43.512 0.075 1927 -1929 2 88.2445 -131.915 43.7452 0.075 1928 -1930 2 90.4546 -134.012 44.0327 0.075 1929 -1931 2 92.5892 -136.087 44.6794 0.075 1930 -1932 2 94.5048 -138.044 46.0033 0.075 1931 -1933 2 96.5858 -140.096 46.7957 0.075 1932 -1934 2 98.743 -142.191 47.3621 0.075 1933 -1935 2 100.946 -144.296 47.6407 0.075 1934 -1936 2 103.206 -146.351 47.8222 0.075 1935 -1937 2 105.489 -148.387 47.9146 0.075 1936 -1938 2 107.751 -150.446 47.9987 0.075 1937 -1939 2 110.008 -152.51 48.11 0.075 1938 -1940 2 112.311 -154.494 48.419 0.075 1939 -1941 2 114.518 -156.447 47.9008 0.075 1940 -1942 2 116.52 -158.489 46.8165 0.075 1941 -1943 2 118.639 -160.596 46.1797 0.075 1942 -1944 2 120.822 -162.734 46.0125 0.075 1943 -1945 2 122.991 -164.891 45.9364 0.075 1944 -1946 2 125.147 -167.063 45.9308 0.075 1945 -1947 2 126.13 -168.591 44.6362 0.075 1946 -1948 2 125.662 -169.229 41.6801 0.075 1947 -1949 2 124.971 -169.736 38.7487 0.075 1948 -1950 2 123.269 -169.072 36.4491 0.075 1949 -1951 2 120.793 -167.595 35.5594 0.075 1950 -1952 2 63.6597 -107.457 42.436 0.075 1917 -1953 2 65.3642 -109.8 43.7453 0.075 1952 -1954 2 66.4431 -110.846 46.9117 0.075 1953 -1955 2 67.5346 -111.657 50.1552 0.075 1954 -1956 2 68.8656 -112.289 53.3503 0.075 1955 -1957 2 54.3087 -107.061 37.1881 0.075 1915 -1958 2 52.132 -108.154 35.1074 0.075 1957 -1959 2 49.6376 -107.889 33.1046 0.075 1958 -1960 2 46.9485 -107.181 31.4941 0.075 1959 -1961 2 43.8485 -107.243 31.2518 0.075 1960 -1962 2 40.6822 -107.588 31.8959 0.075 1961 -1963 2 37.5077 -107.926 32.5085 0.075 1962 -1964 2 34.2769 -108.134 32.7157 0.075 1963 -1965 2 31.4518 -108.434 34.1185 0.075 1964 -1966 2 29.3932 -108.834 36.5991 0.075 1965 -1967 2 26.9526 -109.041 38.6887 0.075 1966 -1968 2 23.9183 -109.092 39.2571 0.075 1967 -1969 2 20.7386 -109.1 38.6214 0.075 1968 -1970 2 17.9001 -108.744 37.1349 0.075 1969 -1971 2 46.5253 -100.824 36.2583 0.075 1912 -1972 2 46.1873 -99.3446 39.0784 0.075 1971 -1973 2 45.9583 -97.5383 41.6933 0.075 1972 -1974 2 45.3949 -94.9595 43.3638 0.075 1973 -1975 2 44.5154 -91.9137 43.7236 0.075 1974 -1976 2 44.2667 -88.8765 44.0802 0.075 1975 -1977 2 44.9107 -85.9238 45.1329 0.075 1976 -1978 2 45.1768 -82.8878 46.0402 0.075 1977 -1979 2 44.9398 -79.7946 46.8168 0.075 1978 -1980 2 45.1736 -77.2921 48.6585 0.075 1979 -1981 2 46.2029 -74.5982 49.9676 0.075 1980 -1982 2 47.387 -71.6539 50.3396 0.075 1981 -1983 2 50.3801 -73.2561 51.2231 0.075 1982 -1984 2 52.9418 -73.7844 53.5882 0.075 1983 -1985 2 55.7579 -75.1536 54.9941 0.075 1984 -1986 2 58.7226 -77.0272 55.4523 0.075 1985 -1987 2 62.0912 -77.8474 55.6327 0.075 1986 -1988 2 65.5472 -77.212 55.6711 0.075 1987 -1989 2 68.1372 -82.492 57.0844 0.075 1988 -1990 2 67.5413 -77.1944 53.3284 0.075 1988 -1991 2 70.5583 -76.9958 52.8352 0.075 1990 -1992 2 73.5863 -77.346 52.6747 0.075 1991 -1993 2 76.4641 -78.416 52.5059 0.075 1992 -1994 2 79.3347 -79.4452 52.1105 0.075 1993 -1995 2 82.1393 -80.625 51.7149 0.075 1994 -1996 2 84.0437 -81.6611 53.4363 0.075 1995 -1997 2 85.8699 -83.0001 55.4966 0.075 1996 -1998 2 87.7583 -85.4011 55.729 0.075 1997 -1999 2 89.4716 -87.7893 56.4524 0.075 1998 -2000 2 91.315 -89.4824 58.2043 0.075 1999 -2001 2 93.7776 -90.6751 59.3843 0.075 2000 -2002 2 96.605 -91.8599 59.6131 0.075 2001 -2003 2 99.0852 -93.4662 60.2552 0.075 2002 -2004 2 101.018 -95.517 61.4529 0.075 2003 -2005 2 102.156 -97.7159 60.3625 0.075 2004 -2006 2 102.597 -99.3889 57.8292 0.075 2005 -2007 2 103.558 -101.639 56.2045 0.075 2006 -2008 2 105.076 -104.203 55.526 0.075 2007 -2009 2 106.661 -106.705 54.7953 0.075 2008 -2010 2 108.56 -109.106 54.715 0.075 2009 -2011 2 110.569 -111.435 54.8084 0.075 2010 -2012 2 112.495 -113.832 54.8322 0.075 2011 -2013 2 114.339 -116.295 54.8329 0.075 2012 -2014 2 115.794 -119 54.9578 0.075 2013 -2015 2 117.027 -121.809 55.1013 0.075 2014 -2016 2 118.565 -124.465 54.9545 0.075 2015 -2017 2 120.167 -127.068 55.2238 0.075 2016 -2018 2 121.673 -129.684 55.8118 0.075 2017 -2019 2 123.43 -132.081 55.7291 0.075 2018 -2020 2 125.471 -134.27 55.0179 0.075 2019 -2021 2 127.19 -136.751 54.4904 0.075 2020 -2022 2 128.385 -139.538 54.7538 0.075 2021 -2023 2 129.415 -141.884 56.3941 0.075 2022 -2024 2 47.0579 -69.3705 52.779 0.075 1982 -2025 2 47.1662 -67.56 55.2167 0.075 2024 -2026 2 46.2851 -64.2836 55.9517 0.075 2025 -2027 2 46.4419 -60.9657 56.7024 0.075 2026 -2028 2 46.7084 -57.5466 56.958 0.075 2027 -2029 2 46.7577 -54.0716 57.0873 0.075 2028 -2030 2 46.1783 -51.1649 58.7681 0.075 2029 -2031 2 45.67 -51.2347 55.648 0.075 2030 -2032 2 45.0917 -51.2562 52.5605 0.075 2031 -2033 2 43.0687 -50.5186 50.4911 0.075 2032 -2034 2 40.0196 -49.9244 49.9373 0.075 2033 -2035 2 37.0656 -49.3912 49.0913 0.075 2034 -2036 2 34.6416 -48.7065 47.2563 0.075 2035 -2037 2 32.7718 -47.9635 44.8322 0.075 2036 -2038 2 31.8351 -46.9664 42.028 0.075 2037 -2039 2 31.9383 -46.3598 38.9539 0.075 2038 -2040 2 32.3834 -46.033 35.8415 0.075 2039 -2041 2 46.4056 -48.4069 59.3182 0.075 2030 -2042 2 46.4013 -46.5909 61.933 0.075 2041 -2043 2 46.4743 -44.9618 64.6536 0.075 2042 -2044 2 46.5358 -42.7395 66.7941 0.075 2043 -2045 2 46.1505 -39.7013 67.0464 0.075 2044 -2046 2 45.8719 -36.6002 67.5976 0.075 2045 -2047 2 46.2776 -33.9695 69.3437 0.075 2046 -2048 2 46.756 -31.5968 71.3586 0.075 2047 -2049 2 47.1539 -29.6296 73.825 0.075 2048 -2050 2 47.3068 -26.9315 75.0053 0.075 2049 -2051 2 46.8265 -23.7969 75.1594 0.075 2050 -2052 2 46.676 -20.8588 76.2739 0.075 2051 -2053 2 46.6346 -18.0538 77.6715 0.075 2052 -2054 2 47.5162 -16.2533 80.1454 0.075 2053 -2055 2 48.2549 -14.0555 82.1116 0.075 2054 -2056 2 48.5679 -11.0355 83.0622 0.075 2055 -2057 2 49.2249 -8.15967 84.1957 0.075 2056 -2058 2 49.7448 -5.3387 85.5563 0.075 2057 -2059 2 49.9271 -2.49303 86.9712 0.075 2058 -2060 2 50.1469 0.426071 88.1186 0.075 2059 -2061 2 51.093 2.51975 90.267 0.075 2060 -2062 2 52.3619 4.72503 92.1225 0.075 2061 -2063 2 53.6761 6.97421 93.8912 0.075 2062 -2064 2 54.6669 9.60659 95.066 0.075 2063 -2065 2 55.0977 12.649 95.72 0.075 2064 -2066 2 55.7548 14.9902 97.5004 0.075 2065 -2067 2 56.7956 16.9187 99.7594 0.075 2066 -2068 2 57.8791 19.1206 101.727 0.075 2067 -2069 2 58.6152 21.9529 102.85 0.075 2068 -2070 2 58.4961 24.6848 104.37 0.075 2069 -2071 2 57.8999 27.6184 105.217 0.075 2070 -2072 2 58.1022 30.5321 106.005 0.075 2071 -2073 2 59.0514 33.1114 107.538 0.075 2072 -2074 2 59.9564 35.8047 108.88 0.075 2073 -2075 2 60.2435 38.0838 110.951 0.075 2074 -2076 2 60.5619 40.1462 113.3 0.075 2075 -2077 2 59.7482 42.9483 114.482 0.075 2076 -2078 2 59.1947 45.7789 115.727 0.075 2077 -2079 2 59.0128 48.6737 116.888 0.075 2078 -2080 2 59.1921 51.1771 118.756 0.075 2079 -2081 2 59.5918 53.4812 120.862 0.075 2080 -2082 2 60.183 56.0089 122.626 0.075 2081 -2083 2 59.918 58.8281 123.899 0.075 2082 -2084 2 58.9028 61.7229 124.571 0.075 2083 -2085 2 59.2539 64.4284 126.188 0.075 2084 -2086 2 59.4552 67.1736 127.763 0.075 2085 -2087 2 59.8105 69.8228 129.426 0.075 2086 -2088 2 60.0104 72.2495 131.381 0.075 2087 -2089 2 59.603 75.0151 132.821 0.075 2088 -2090 2 59.8582 77.8823 134.086 0.075 2089 -2091 2 60.2386 80.7195 135.449 0.075 2090 -2092 2 60.3992 83.8132 136.073 0.075 2091 -2093 2 60.1486 86.9584 136.006 0.075 2092 -2094 2 59.238 89.9619 135.687 0.075 2093 -2095 2 58.0242 92.8824 135.659 0.075 2094 -2096 2 56.7306 95.7176 136.186 0.075 2095 -2097 2 55.3648 98.4836 136.921 0.075 2096 -2098 2 54.2968 101.454 137.183 0.075 2097 -2099 2 53.4875 104.477 137.663 0.075 2098 -2100 2 52.801 107.505 138.156 0.075 2099 -2101 2 52.064 110.584 138.082 0.075 2100 -2102 2 50.8939 113.522 138.003 0.075 2101 -2103 2 50.0859 116.57 137.861 0.075 2102 -2104 2 49.231 119.621 137.757 0.075 2103 -2105 2 48.2737 122.631 138.015 0.075 2104 -2106 2 47.6152 125.66 138.603 0.075 2105 -2107 2 47.3147 128.412 140.048 0.075 2106 -2108 2 46.9223 130.97 141.852 0.075 2107 -2109 2 45.9721 133.845 142.451 0.075 2108 -2110 2 45.0502 136.864 142.199 0.075 2109 -2111 2 46.0907 137.737 139.283 0.075 2110 -2112 2 47.2284 138.447 136.368 0.075 2111 -2113 2 48.6861 138.58 133.503 0.075 2112 -2114 2 50.2802 138.846 130.738 0.075 2113 -2115 2 52.4977 139.866 128.895 0.075 2114 -2116 2 55.1077 141.561 128.11 0.075 2115 -2117 2 57.5982 143.538 127.657 0.075 2116 -2118 2 60.3294 145.205 127.473 0.075 2117 -2119 2 63.3919 146.161 127.467 0.075 2118 -2120 2 65.8592 146.983 125.597 0.075 2119 -2121 2 68.1631 147.792 123.867 0.075 2120 -2122 2 70.6035 149.07 125.088 0.075 2121 -2123 2 72.6844 150.39 127.156 0.075 2122 -2124 2 75.0631 152.175 128.243 0.075 2123 -2125 2 77.5987 154.132 128.445 0.075 2124 -2126 2 43.6251 138.913 140.322 0.075 2110 -2127 2 42.3053 141.698 139.79 0.075 2126 -2128 2 41.2231 144.596 139.812 0.075 2127 -2129 2 40.1256 147.412 140.61 0.075 2128 -2130 2 39.164 150.307 141.311 0.075 2129 -2131 2 37.8645 153.116 141.756 0.075 2130 -2132 2 36.6798 155.817 142.765 0.075 2131 -2133 2 36.2417 158.458 144.373 0.075 2132 -2134 2 36.0037 161.336 145.552 0.075 2133 -2135 2 35.0161 164.077 146.681 0.075 2134 -2136 2 33.6529 166.721 147.643 0.075 2135 -2137 2 32.1827 169.447 147.844 0.075 2136 -2138 2 31.0049 172.341 147.719 0.075 2137 -2139 2 29.9494 175.277 147.977 0.075 2138 -2140 2 28.405 177.494 149.562 0.075 2139 -2141 2 26.8633 179.598 151.294 0.075 2140 -2142 2 25.3328 181.898 152.723 0.075 2141 -2143 2 23.3699 184.144 153.663 0.075 2142 -2144 2 21.4884 186.134 155.112 0.075 2143 -2145 2 19.7272 188.145 156.747 0.075 2144 -2146 2 17.7566 190.405 157.618 0.075 2145 -2147 2 15.5664 192.63 157.818 0.075 2146 -2148 2 13.3676 194.855 157.635 0.075 2147 -2149 2 11.1436 197.032 157.785 0.075 2148 -2150 2 9.82629 198.77 159.644 0.075 2149 -2151 2 9.03975 200.252 162.291 0.075 2150 -2152 2 8.26417 201.887 164.849 0.075 2151 -2153 2 56.6924 60.9193 126.547 0.075 2084 -2154 2 54.8589 60.3445 128.938 0.075 2153 -2155 2 52.6029 59.5485 130.79 0.075 2154 -2156 2 49.7083 59.263 131.606 0.075 2155 -2157 2 46.8273 60.0322 132.133 0.075 2156 -2158 2 44.2132 61.3996 132.976 0.075 2157 -2159 2 41.499 62.5838 133.759 0.075 2158 -2160 2 38.662 63.502 134.496 0.075 2159 -2161 2 35.7272 63.9223 135.284 0.075 2160 -2162 2 32.9144 64.3725 136.365 0.075 2161 -2163 2 30.7342 65.4238 138.252 0.075 2162 -2164 2 28.508 66.7831 139.795 0.075 2163 -2165 2 25.8313 68.0759 140.528 0.075 2164 -2166 2 22.9762 69.1444 140.869 0.075 2165 -2167 2 20.1803 70.0808 141.604 0.075 2166 -2168 2 18.0531 70.8853 143.582 0.075 2167 -2169 2 16.1112 71.6673 145.831 0.075 2168 -2170 2 13.724 72.0702 147.716 0.075 2169 -2171 2 11.0899 72.1524 149.262 0.075 2170 -2172 2 8.23381 71.5948 150.201 0.075 2171 -2173 2 5.36896 70.7069 150.869 0.075 2172 -2174 2 2.90305 69.75 152.236 0.075 2173 -2175 2 1.41892 68.7312 154.677 0.075 2174 -2176 2 0.078237 67.6653 157.227 0.075 2175 -2177 2 -1.92558 66.4722 159.205 0.075 2176 -2178 2 -4.58278 65.323 160.231 0.075 2177 -2179 2 -7.22643 64.392 161.482 0.075 2178 -2180 2 -9.94832 63.9044 162.773 0.075 2179 -2181 2 -12.6656 63.886 164.148 0.075 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2 48.0516 -7.76776 11.0289 0.075 2902 -2904 2 48.5331 -4.44431 10.9419 0.075 2903 -2905 2 49.5077 -1.25004 10.5295 0.075 2904 -2906 2 50.3863 1.86854 9.65842 0.075 2905 -2907 2 50.8635 5.03407 8.60607 0.075 2906 -2908 2 50.6931 8.31663 7.92545 0.075 2907 -2909 2 50.4151 11.6473 7.47276 0.075 2908 -2910 2 47.1151 15.0573 7.25943 0.075 2909 -2911 2 46.8487 18.371 6.81835 0.075 2910 -2912 2 45.4615 21.2812 5.88966 0.075 2911 -2913 2 43.4009 23.6521 4.76494 0.075 2912 -2914 2 41.3764 25.0829 2.66436 0.075 2913 -2915 2 39.8425 25.9631 -0.195264 0.075 2914 -2916 2 38.2967 26.8206 -3.04258 0.075 2915 -2917 2 35.8915 27.9629 -5.07622 0.075 2916 -2918 2 33.4127 29.4695 -6.7317 0.075 2917 -2919 2 31.0924 31.1479 -8.49472 0.075 2918 -2920 2 28.5479 32.7834 -9.96262 0.075 2919 -2921 2 27.3812 33.7816 -13.0134 0.075 2920 -2922 2 25.9457 35.0784 -15.827 0.075 2921 -2923 2 24.5492 36.1497 -18.7536 0.075 2922 -2924 2 22.2087 37.3502 -20.921 0.075 2923 -2925 2 24.0158 39.9656 -23.0232 0.075 2924 -2926 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131.049 19.3327 0.075 4891 -4893 2 14.1836 133.884 20.0782 0.075 4892 -4894 2 14.6964 135.908 22.2536 0.075 4893 -4895 2 15.1593 137.768 24.6504 0.075 4894 -4896 2 28.8434 -3.24301 2.1695 0.075 4834 -4897 2 29.9368 -1.28311 -0.0127656 0.075 4896 -4898 2 31.3422 0.346656 -2.28268 0.075 4897 -4899 2 32.8043 1.46032 -4.81057 0.075 4898 -4900 2 33.8868 2.16336 -7.64738 0.075 4899 -4901 2 34.7248 2.14293 -10.6471 0.075 4900 -4902 2 35.2905 1.39215 -13.6144 0.075 4901 -4903 2 35.6714 -0.0408053 -16.3569 0.075 4902 -4904 2 36.3044 -1.75792 -18.8885 0.075 4903 -4905 2 37.265 -3.38354 -21.3875 0.075 4904 -4906 2 37.886 -4.71837 -24.1384 0.075 4905 -4907 2 38.9071 -5.83977 -26.8706 0.075 4906 -4908 2 39.9097 -6.56819 -29.7465 0.075 4907 -4909 2 37.7676 -46.6283 24.5153 0.075 4666 -4910 2 34.5355 -46.4781 27.7168 0.075 4909 -4911 2 32.5612 -46.4244 31.8235 0.075 4910 -4912 2 31.514 -47.7346 29.1506 0.075 4911 -4913 2 30.4052 -49.1615 26.5639 0.075 4912 -4914 2 29.4428 -50.418 23.8479 0.075 4913 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38.1822 0.075 4959 -4961 2 53.9724 -64.0518 38.0037 0.075 4960 -4962 2 55.872 -64.5261 35.6877 0.075 4961 -4963 2 57.8753 -65.0566 33.5059 0.075 4962 -4964 2 60.3101 -66.1618 32.2306 0.075 4963 -4965 2 62.9436 -67.4847 31.5026 0.075 4964 -4966 2 65.4436 -69.0715 30.8551 0.075 4965 -4967 2 67.9232 -70.7594 30.3922 0.075 4966 -4968 2 70.337 -72.304 29.4236 0.075 4967 -4969 2 72.6582 -73.3595 27.7774 0.075 4968 -4970 2 75.0504 -74.375 26.2344 0.075 4969 -4971 2 77.6527 -75.6419 25.4251 0.075 4970 -4972 2 80.4203 -76.8045 25.0365 0.075 4971 -4973 2 83.1886 -77.6379 24.1703 0.075 4972 -4974 2 85.9493 -78.4459 23.2027 0.075 4973 -4975 2 88.6741 -79.446 22.3161 0.075 4974 -4976 2 91.4099 -80.4534 21.4897 0.075 4975 -4977 2 94.2799 -81.3441 21.1018 0.075 4976 -4978 2 97.0433 -82.3179 20.3217 0.075 4977 -4979 2 99.8444 -83.0146 19.3923 0.075 4978 -4980 2 102.638 -83.5569 18.3429 0.075 4979 -4981 2 105.204 -84.6082 17.1745 0.075 4980 -4982 2 107.691 -86.1747 16.4816 0.075 4981 -4983 2 110.272 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1812 -1814 2 -46.6999 -139.543 -1.35524 0.075 1813 -1815 2 -49.6566 -140.234 -0.602308 0.075 1814 -1816 2 -52.685 -140.112 0.262559 0.075 1815 -1817 2 3.71387 -110.779 3.42817 0.075 1789 -1818 2 2.97161 -107.782 3.40845 0.075 1817 -1819 2 1.96144 -104.843 3.13981 0.075 1818 -1820 2 0.627151 -102.133 3.43795 0.075 1819 -1821 2 -0.701405 -99.557 4.4962 0.075 1820 -1822 2 -1.27354 -96.731 5.69512 0.075 1821 -1823 2 -2.06943 -93.7463 6.16177 0.075 1822 -1824 2 -3.31925 -90.9129 6.03985 0.075 1823 -1825 2 -4.23966 -87.98 5.70495 0.075 1824 -1826 2 -4.61738 -84.9122 5.32017 0.075 1825 -1827 2 -4.61188 -81.8129 4.93086 0.075 1826 -1828 2 -4.99745 -78.7338 4.57018 0.075 1827 -1829 2 -5.74686 -75.7259 4.24353 0.075 1828 -1830 2 -6.63371 -72.7469 3.93062 0.075 1829 -1831 2 -7.35238 -69.7437 3.60194 0.075 1830 -1832 2 -7.4818 -66.6464 3.21533 0.075 1831 -1833 2 -8.86653 -63.3945 2.96038 0.075 1832 -1834 2 -10.7411 -60.4264 2.97127 0.075 1833 -1835 2 -12.9091 -57.6285 3.14142 0.075 1834 -1836 2 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56.4746 0.075 1857 -1859 2 -16.1576 -91.5501 56.3 0.075 1858 -1860 2 -18.0254 -93.8096 55.3759 0.075 1859 -1861 2 -18.6779 -96.7127 54.2345 0.075 1860 -1862 2 -19.0218 -98.9106 51.9511 0.075 1861 -1863 2 -19.2555 -101.152 49.6976 0.075 1862 -1864 2 -19.2351 -103.176 47.2576 0.075 1863 -1865 2 -18.9125 -104.79 44.5548 0.075 1864 -1866 2 -18.3634 -105.955 41.6391 0.075 1865 -1867 2 -17.8959 -107.02 38.6707 0.075 1866 -1868 2 -17.3942 -108.16 35.7364 0.075 1867 -1869 2 -16.7911 -109.774 33.0821 0.075 1868 -1870 2 -16.0628 -111.571 30.5538 0.075 1869 -1871 2 -15.0859 -113.347 28.121 0.075 1870 -1872 2 -13.5511 -115.275 26.0986 0.075 1871 -1873 2 -18.2389 -94.23 58.4721 0.075 1860 -1874 2 -19.0715 -95.75 61.0781 0.075 1873 -1875 2 -20.1274 -97.9229 63.0514 0.075 1874 -1876 2 -20.333 -99.2991 65.738 0.075 1875 -1877 2 -20.4237 -100.198 65.2994 0.075 1876 -1878 2 -20.6017 -100.666 62.2106 0.075 1877 -1879 2 -20.313 -101.211 61.3692 0.075 1878 -1880 2 -19.7478 -101.322 64.4477 0.075 1879 -1881 2 -19.1828 -101.433 67.5262 0.075 1880 -1882 2 -18.6178 -101.543 70.6047 0.075 1881 -1883 2 -17.6056 -102.411 73.38 0.075 1882 -1884 2 -16.6585 -103.594 76.1119 0.075 1883 -1885 2 -16.3491 -104.537 78.9975 0.075 1884 -1886 2 -16.929 -105.046 82.0243 0.075 1885 -1887 2 -17.9769 -105.69 84.9045 0.075 1886 -1888 2 -19.2389 -107.365 87.2176 0.075 1887 -1889 2 -20.3327 -110.05 88.0124 0.075 1888 -1890 2 -21.1869 -113.027 88.443 0.075 1889 -1891 2 -21.8366 -116.056 88.896 0.075 1890 -1892 2 -22.3985 -119.077 89.4715 0.075 1891 -1893 2 -23.0775 -121.983 90.4204 0.075 1892 -1894 2 -23.4766 -124.769 91.791 0.075 1893 -1895 2 -23.3296 -127.104 93.8199 0.075 1894 -1896 2 -23.0461 -129.336 95.9986 0.075 1895 -1897 2 -22.5995 -131.691 97.9841 0.075 1896 -1898 2 -21.7722 -134.283 99.4488 0.075 1897 -1899 2 -20.4543 -137.057 100.064 0.075 1898 -1900 2 -13.7656 -54.2099 2.35623 0.075 1835 -1901 2 -14.4342 -50.8478 1.22189 0.075 1900 -1902 2 -15.4417 -47.5262 0.245638 0.075 1901 -1903 2 -16.6985 -44.2116 -0.375186 0.075 1902 -1904 2 -17.935 -40.8448 -0.749218 0.075 1903 -1905 2 -18.8406 -37.3721 -1.03621 0.075 1904 -1906 2 -19.2179 -33.7835 -1.16579 0.075 1905 -1907 2 -20.359 -30.8473 -1.31561 0.075 1906 -1908 2 -20.895 -27.8113 -1.99553 0.075 1907 -1909 2 -21.144 -24.735 -2.64921 0.075 1908 -1910 2 -20.9841 -21.6143 -2.82256 0.075 1909 -1911 2 -20.8274 -18.5063 -2.4414 0.075 1910 -1912 2 -21.1339 -15.4468 -1.93523 0.075 1911 -1913 2 -22.1435 -12.4759 -1.96025 0.075 1912 -1914 2 -23.6383 -9.74973 -2.5086 0.075 1913 -1915 2 -24.8249 -6.89042 -3.12787 0.075 1914 -1916 2 -25.7411 -4.16883 -4.41977 0.075 1915 -1917 2 -26.5247 -1.23549 -5.19726 0.075 1916 -1918 2 -27.4952 1.70858 -5.10308 0.075 1917 -1919 2 -28.4539 4.67614 -4.82483 0.075 1918 -1920 2 -29.1587 7.69727 -5.20461 0.075 1919 -1921 2 -29.8518 10.5627 -6.33301 0.075 1920 -1922 2 -30.7325 13.3979 -7.40672 0.075 1921 -1923 2 -31.7046 16.3303 -8.03693 0.075 1922 -1924 2 -32.6843 19.2971 -8.48263 0.075 1923 -1925 2 -33.604 22.2851 -8.91647 0.075 1924 -1926 2 -34.5448 25.2545 -9.4187 0.075 1925 -1927 2 -35.7162 28.1297 -9.9337 0.075 1926 -1928 2 -37.3411 30.8204 -10.2281 0.075 1927 -1929 2 -39.898 31.6295 -13.5872 0.075 1928 -1930 2 -42.7534 32.7274 -16.6106 0.075 1929 -1931 2 -43.8725 35.4776 -15.7273 0.075 1930 -1932 2 -45.364 38.0627 -16.5535 0.075 1931 -1933 2 -46.392 40.4544 -18.1613 0.075 1932 -1934 2 -47.3624 43.0184 -19.5206 0.075 1933 -1935 2 -48.3779 45.8223 -20.3174 0.075 1934 -1936 2 -49.3518 48.734 -20.7277 0.075 1935 -1937 2 -50.1512 51.7004 -21.1245 0.075 1936 -1938 2 -50.767 54.7122 -21.4923 0.075 1937 -1939 2 -51.3141 57.7405 -21.8472 0.075 1938 -1940 2 -51.896 60.7438 -22.2957 0.075 1939 -1941 2 -52.6178 63.6649 -23.0272 0.075 1940 -1942 2 -53.1603 66.2393 -24.6625 0.075 1941 -1943 2 -53.8784 68.6458 -26.4684 0.075 1942 -1944 2 -54.7652 71.1046 -28.1097 0.075 1943 -1945 2 -55.8375 73.674 -29.4539 0.075 1944 -1946 2 -57.1334 76.2671 -30.5458 0.075 1945 -1947 2 -58.5468 78.9111 -31.3245 0.075 1946 -1948 2 -60.081 81.4799 -32.1222 0.075 1947 -1949 2 -61.7395 83.8387 -33.2005 0.075 1948 -1950 2 -63.4862 85.9946 -34.5772 0.075 1949 -1951 2 -65.2452 88.1083 -36.0036 0.075 1950 -1952 2 -45.2734 32.5637 -18.9139 0.075 1930 -1953 2 -47.3044 33.5103 -21.6019 0.075 1952 -1954 2 -49.6605 33.7451 -24.1787 0.075 1953 -1955 2 -52.4899 33.3202 -26.0934 0.075 1954 -1956 2 -55.4778 32.6192 -27.7745 0.075 1955 -1957 2 -58.2555 32.3076 -29.6304 0.075 1956 -1958 2 -60.2704 33.6731 -32.1448 0.075 1957 -1959 2 -36.2211 33.4904 -10.6281 0.075 1928 -1960 2 -34.414 34.7072 -8.08377 0.075 1959 -1961 2 -33.1227 34.724 -4.95338 0.075 1960 -1962 2 -31.5007 34.747 -1.98133 0.075 1961 -1963 2 -30.0423 34.2935 1.03362 0.075 1962 -1964 2 -29.0951 33.5414 4.18025 0.075 1963 -1965 2 -28.2315 32.8498 7.37827 0.075 1964 -1966 2 -27.2617 32.1149 10.5351 0.075 1965 -1967 2 -26.263 31.3574 13.6773 0.075 1966 -1968 2 -25.4154 31.1219 16.8912 0.075 1967 -1969 2 -24.8711 31.8607 20.1509 0.075 1968 -1970 2 -24.3267 32.5994 23.4105 0.075 1969 -1971 2 -38.3946 36.0822 -11.0723 0.075 1959 -1972 2 -39.1833 39.2496 -12.0631 0.075 1971 -1973 2 -39.8925 42.478 -12.9029 0.075 1972 -1974 2 -40.4606 45.7818 -13.5035 0.075 1973 -1975 2 -40.7113 49.1411 -13.9554 0.075 1974 -1976 2 -40.56 52.5227 -14.3744 0.075 1975 -1977 2 -40.2739 55.9035 -14.7312 0.075 1976 -1978 2 -38.3257 57.3909 -11.8939 0.075 1977 -1979 2 -36.2941 58.8851 -9.12034 0.075 1978 -1980 2 -34.2157 60.3831 -6.38264 0.075 1979 -1981 2 -43.0232 60.195 -14.927 0.075 1977 -1982 2 -44.9004 64.89 -15.7007 0.075 1981 -1983 2 -43.6339 66.7063 -18.07 0.075 1982 -1984 2 -42.8479 68.9154 -20.3095 0.075 1983 -1985 2 -41.8219 71.558 -21.8138 0.075 1984 -1986 2 -40.6888 74.3643 -22.9712 0.075 1985 -1987 2 -39.5598 77.1249 -24.2146 0.075 1986 -1988 2 -38.495 79.4536 -26.032 0.075 1987 -1989 2 -37.4653 80.4758 -28.9322 0.075 1988 -1990 2 -35.4741 82.3903 -30.6163 0.075 1989 -1991 2 -33.2627 84.3016 -32.009 0.075 1990 -1992 2 -30.9132 85.7659 -33.6303 0.075 1991 -1993 2 -28.4986 86.6501 -35.5769 0.075 1992 -1994 2 -25.9574 86.9945 -37.4678 0.075 1993 -1995 2 -22.8476 86.7119 -38.343 0.075 1994 -1996 2 -46.4474 67.9306 -15.6531 0.075 1982 -1997 2 -47.2131 70.9907 -14.4368 0.075 1996 -1998 2 -48.0254 74.0563 -13.185 0.075 1997 -1999 2 -49.14 77.1026 -12.4283 0.075 1998 -2000 2 -50.7531 80.0992 -12.671 0.075 1999 -2001 2 -54.034 80.1089 -12.262 0.075 2000 -2002 2 -56.5217 79.864 -14.4464 0.075 2001 -2003 2 -59.6428 79.3112 -14.8931 0.075 2002 -2004 2 -62.5898 78.6429 -13.7394 0.075 2003 -2005 2 -65.4912 77.5775 -12.7641 0.075 2004 -2006 2 -68.4555 76.1348 -12.5894 0.075 2005 -2007 2 -71.4148 74.6967 -13.03 0.075 2006 -2008 2 -50.6379 83.0336 -13.6299 0.075 2000 -2009 2 -51.525 85.4755 -15.3449 0.075 2008 -2010 2 -52.4792 88.0011 -16.8936 0.075 2009 -2011 2 -53.2877 90.893 -17.5461 0.075 2010 -2012 2 -53.9755 93.9036 -17.9329 0.075 2011 -2013 2 -54.6455 96.9201 -18.3082 0.075 2012 -2014 2 -55.3643 99.9259 -18.682 0.075 2013 -2015 2 -56.1032 102.918 -19.0759 0.075 2014 -2016 2 -56.9248 105.738 -20.1072 0.075 2015 -2017 2 -57.5283 108.569 -21.2516 0.075 2016 -2018 2 -58.2222 111.43 -22.2555 0.075 2017 -2019 2 -58.8803 114.333 -23.1646 0.075 2018 -2020 2 -59.4306 117.316 -23.7451 0.075 2019 -2021 2 -59.9779 120.363 -23.6839 0.075 2020 -2022 2 -60.7599 123.323 -23.1304 0.075 2021 -2023 2 -61.463 126.355 -23.141 0.075 2022 -2024 2 -61.7093 129.426 -23.5325 0.075 2023 -2025 2 -61.3144 132.424 -24.1581 0.075 2024 -2026 2 -60.9881 135.432 -24.8297 0.075 2025 -2027 2 -61.2328 138.459 -25.3731 0.075 2026 -2028 2 -62.0207 141.431 -25.5147 0.075 2027 -2029 2 -62.8903 144.304 -24.6914 0.075 2028 -2030 2 -64.033 147.065 -23.8185 0.075 2029 -2031 2 -65.2968 149.89 -23.7217 0.075 2030 -2032 2 -66.5798 152.612 -24.3899 0.075 2031 -2033 2 -67.8389 155.116 -25.6943 0.075 2032 -2034 2 -68.8328 157.353 -27.5531 0.075 2033 -2035 2 -69.492 159.146 -29.9362 0.075 2034 -2036 2 -70.7528 159.818 -32.7021 0.075 2035 -2037 2 -72.6371 160.189 -35.1521 0.075 2036 -2038 2 -74.2116 161.012 -37.6957 0.075 2037 -2039 2 -75.2427 162.736 -39.9673 0.075 2038 -2040 2 -76.1785 165.156 -41.5817 0.075 2039 -2041 2 -77.1112 167.856 -42.7939 0.075 2040 -2042 2 -77.7483 170.58 -44.1575 0.075 2041 -2043 2 -78.3175 173.377 -45.4011 0.075 2042 -2044 2 -18.3482 -31.817 1.29288 0.075 1906 -2045 2 -18.8488 -28.5478 0.917441 0.075 2044 -2046 2 -18.715 -25.2518 0.599457 0.075 2045 -2047 2 -18.2578 -21.9643 0.43709 0.075 2046 -2048 2 -17.8128 -18.6835 0.566987 0.075 2047 -2049 2 -17.5376 -15.4052 0.989401 0.075 2048 -2050 2 -16.6505 -12.197 0.982169 0.075 2049 -2051 2 -15.4397 -9.1298 0.563962 0.075 2050 -2052 2 -15.0066 -5.91907 0.059005 0.075 2051 -2053 2 -15.2772 -2.67258 -0.526285 0.075 2052 -2054 2 -15.8067 0.534005 -1.21156 0.075 2053 -2055 2 -15.9513 3.76558 -1.99609 0.075 2054 -2056 2 -18.56 4.67126 -3.57217 0.075 2055 -2057 2 -21.6862 4.53261 -4.30942 0.075 2056 -2058 2 -24.3234 4.87089 -6.09464 0.075 2057 -2059 2 -26.8197 5.62211 -7.96433 0.075 2058 -2060 2 -29.2845 6.82958 -9.60534 0.075 2059 -2061 2 -31.8195 8.43253 -10.6102 0.075 2060 -2062 2 -34.3516 10.3263 -10.6824 0.075 2061 -2063 2 -36.5882 12.323 -10.4044 0.075 2062 -2064 2 -38.4313 13.8988 -12.513 0.075 2063 -2065 2 -40.1817 15.3075 -14.8126 0.075 2064 -2066 2 -42.4026 16.9653 -16.4306 0.075 2065 -2067 2 -44.6792 19.136 -16.7736 0.075 2066 -2068 2 -47.024 21.3324 -16.7499 0.075 2067 -2069 2 -49.4265 23.4654 -16.6378 0.075 2068 -2070 2 -51.7848 25.6422 -16.5824 0.075 2069 -2071 2 -54.035 27.9319 -16.7049 0.075 2070 -2072 2 -56.4475 30.0417 -16.9595 0.075 2071 -2073 2 -14.7026 5.75839 -1.27144 0.075 2055 -2074 2 -14.4065 6.51378 -4.23118 0.075 2073 -2075 2 -14.2059 7.16428 -7.22409 0.075 2074 -2076 2 -13.818 8.85091 -9.7267 0.075 2075 -2077 2 -13.2897 11.4562 -11.0919 0.075 2076 -2078 2 -12.4156 14.2837 -11.7954 0.075 2077 -2079 2 -11.3262 17.1168 -12.2506 0.075 2078 -2080 2 -10.3271 19.9681 -12.7573 0.075 2079 -2081 2 -9.72447 22.9062 -13.4095 0.075 2080 -2082 2 -8.79253 25.723 -14.1909 0.075 2081 -2083 2 -7.55658 28.3921 -15.0463 0.075 2082 -2084 2 -6.66989 30.8552 -14.3857 0.075 2083 -2085 2 -6.0451 33.1778 -12.485 0.075 2084 -2086 2 -5.1112 35.5327 -10.8299 0.075 2085 -2087 2 -3.67363 38.1593 -10.1552 0.075 2086 -2088 2 -2.23119 40.8176 -10.619 0.075 2087 -2089 2 -1.19433 43.6091 -11.313 0.075 2088 -2090 2 -0.635329 46.5545 -11.4675 0.075 2089 -2091 2 -0.481075 49.2803 -10.4513 0.075 2090 -2092 2 -0.447304 51.414 -8.25924 0.075 2091 -2093 2 -0.075483 53.2377 -5.81855 0.075 2092 -2094 2 0.296327 55.0614 -3.37794 0.075 2093 -2095 2 1.00534 57.6927 -2.31055 0.075 2094 -2096 2 1.88628 60.5968 -2.18515 0.075 2095 -2097 2 3.10617 63.3653 -2.45556 0.075 2096 -2098 2 4.92813 65.8129 -2.71037 0.075 2097 -2099 2 7.11082 67.97 -2.77121 0.075 2098 -2100 2 9.20127 70.2135 -2.89844 0.075 2099 -2101 2 11.1532 72.4515 -3.59176 0.075 2100 -2102 2 13.2014 74.5314 -4.51824 0.075 2101 -2103 2 15.4724 76.4333 -5.18361 0.075 2102 -2104 2 17.869 78.3062 -5.10197 0.075 2103 -2105 2 19.6823 80.5884 -4.1407 0.075 2104 -2106 2 21.137 83.1731 -3.36542 0.075 2105 -2107 2 23.1162 85.44 -3.2943 0.075 2106 -2108 2 25.4923 87.3205 -3.65603 0.075 2107 -2109 2 28.0037 88.9234 -4.34028 0.075 2108 -2110 2 30.4094 90.4617 -5.44441 0.075 2109 -2111 2 32.3747 92.4057 -6.77823 0.075 2110 -2112 2 34.3607 94.4363 -7.93971 0.075 2111 -2113 2 36.3476 96.541 -8.95651 0.075 2112 -2114 2 38.2875 98.7446 -9.84459 0.075 2113 -2115 2 40.0399 101.069 -10.7668 0.075 2114 -2116 2 41.3812 103.51 -12.0502 0.075 2115 -2117 2 42.4382 105.803 -13.7956 0.075 2116 -2118 2 43.7111 108.032 -15.4745 0.075 2117 -2119 2 45.0715 110.216 -17.148 0.075 2118 -2120 2 46.3085 112.367 -18.9408 0.075 2119 -2121 2 47.2107 114.58 -20.8423 0.075 2120 -2122 2 47.6377 117.106 -22.5321 0.075 2121 -2123 2 -5.25519 -65.7748 6.10786 0.075 1832 -2124 2 -2.11589 -65.4094 8.02941 0.075 2123 -2125 2 1.06498 -65.316 9.98101 0.075 2124 -2126 2 4.03515 -65.4816 12.269 0.075 2125 -2127 2 48.1654 -175.974 24.484 0.075 1566 -2128 2 47.3781 -178.935 25.7261 0.075 2127 -2129 2 47.7933 -181.974 26.8941 0.075 2128 -2130 2 48.8124 -184.484 28.7105 0.075 2129 -2131 2 50.156 -186.743 30.7091 0.075 2130 -2132 2 51.7619 -189.082 32.3489 0.075 2131 -2133 2 53.6706 -191.574 33.244 0.075 2132 -2134 2 55.85 -194.053 33.4142 0.075 2133 -2135 2 58.064 -196.503 33.5762 0.075 2134 -2136 2 58.6305 -199.57 34.0172 0.075 2135 -2137 2 58.8678 -202.678 34.4734 0.075 2136 -2138 2 58.8904 -204.72 36.6696 0.075 2137 -2139 2 58.8424 -206.402 39.3312 0.075 2138 -2140 2 59.2367 -208.225 41.8048 0.075 2139 -2141 2 60.1962 -210.417 43.8118 0.075 2140 -2142 2 61.3002 -212.864 45.426 0.075 2141 -2143 2 62.3172 -214.761 47.7261 0.075 2142 -2144 2 63.3269 -216.362 50.2441 0.075 2143 -2145 2 64.1883 -218.284 52.5776 0.075 2144 -2146 2 64.7406 -220.71 54.4412 0.075 2145 -2147 2 64.9557 -223.578 55.589 0.075 2146 -2148 2 65.1078 -226.611 56.3395 0.075 2147 -2149 2 65.6875 -229.4 57.6305 0.075 2148 -2150 2 66.4631 -231.509 59.8381 0.075 2149 -2151 2 66.8772 -233.516 62.2278 0.075 2150 -2152 2 66.8792 -235.451 64.7016 0.075 2151 -2153 2 66.4742 -237.349 67.1709 0.075 2152 -2154 2 65.916 -239.067 69.7347 0.075 2153 -2155 2 65.5714 -240.284 72.5844 0.075 2154 -2156 2 65.5711 -240.655 75.7127 0.075 2155 -2157 2 61.06 -196.935 33.4346 0.075 2135 -2158 2 63.5994 -198.594 33.67 0.075 2157 -2159 2 65.827 -199.757 35.2623 0.075 2158 -2160 2 67.8332 -200.751 37.3117 0.075 2159 -2161 2 69.6377 -202.328 38.8956 0.075 2160 -2162 2 71.2918 -204.778 39.5774 0.075 2161 -2163 2 72.9295 -207.327 39.8534 0.075 2162 -2164 2 74.6969 -209.785 40.1561 0.075 2163 -2165 2 76.342 -212.295 40.641 0.075 2164 -2166 2 78.018 -214.689 41.4476 0.075 2165 -2167 2 79.7578 -216.9 42.5856 0.075 2166 -2168 2 81.3693 -219.015 44.0096 0.075 2167 -2169 2 82.2206 -221.401 45.6948 0.075 2168 -2170 2 82.9902 -223.811 47.3842 0.075 2169 -2171 2 83.7067 -226.044 49.3145 0.075 2170 -2172 2 84.6194 -228.172 51.2763 0.075 2171 -2173 2 85.9036 -230.343 52.903 0.075 2172 -2174 2 87.6061 -232.658 53.8596 0.075 2173 -2175 2 89.618 -234.922 54.1458 0.075 2174 -2176 2 91.8167 -236.989 54.5228 0.075 2175 -2177 2 94.0719 -238.947 55.0868 0.075 2176 -2178 2 96.4213 -240.683 55.8936 0.075 2177 -2179 2 98.8259 -242.259 56.8857 0.075 2178 -2180 2 101.149 -243.939 57.9004 0.075 2179 -2181 2 103.308 -245.838 58.8946 0.075 2180 -2182 2 105.32 -248.06 59.3391 0.075 2181 -2183 2 107.866 -249.529 59.4494 0.075 2182 -2184 2 110.797 -250.27 59.5304 0.075 2183 -2185 2 113.792 -250.519 59.8568 0.075 2184 -2186 2 116.736 -250.216 60.5613 0.075 2185 -2187 2 119.616 -250.006 61.5188 0.075 2186 -2188 2 122.523 -250.404 62.253 0.075 2187 -2189 2 125.43 -250.947 62.9674 0.075 2188 -2190 2 128.072 -251.684 64.1037 0.075 2189 -2191 2 130.272 -252.771 65.9026 0.075 2190 -2192 2 132.411 -253.916 67.7371 0.075 2191 -2193 2 134.73 -254.692 69.5453 0.075 2192 -2194 2 137.238 -255.326 71.1351 0.075 2193 -2195 2 139.934 -255.832 72.4255 0.075 2194 -2196 2 142.649 -256.161 73.71 0.075 2195 -2197 2 144.817 -256.172 75.6747 0.075 2196 -2198 2 145.708 -255.853 78.5662 0.075 2197 -2199 2 60.5586 -175.461 28.1239 0.075 1563 -2200 2 63.1517 -177.204 28.6333 0.075 2199 -2201 2 65.9947 -178.418 29.1987 0.075 2200 -2202 2 68.9348 -178.843 30.0828 0.075 2201 -2203 2 71.8542 -178.761 31.0489 0.075 2202 -2204 2 74.9585 -179.28 30.6384 0.075 2203 -2205 2 78.125 -179.289 30.542 0.075 2204 -2206 2 81.279 -179.005 30.5834 0.075 2205 -2207 2 84.3832 -178.653 30.9526 0.075 2206 -2208 2 87.4012 -178.513 31.926 0.075 2207 -2209 2 87.6918 -180.214 34.5534 0.075 2208 -2210 2 88.1304 -182.141 36.9987 0.075 2209 -2211 2 88.7053 -184.207 39.2945 0.075 2210 -2212 2 89.7546 -186.413 41.2214 0.075 2211 -2213 2 91.1604 -188.682 42.8787 0.075 2212 -2214 2 92.5971 -190.963 44.4901 0.075 2213 -2215 2 93.8126 -193.302 46.2031 0.075 2214 -2216 2 94.9174 -195.63 48.004 0.075 2215 -2217 2 95.601 -196.975 50.7191 0.075 2216 -2218 2 96.0716 -197.976 53.6557 0.075 2217 -2219 2 96.6489 -199.195 56.4718 0.075 2218 -2220 2 97.3237 -200.771 59.1032 0.075 2219 -2221 2 97.8225 -202.355 61.7459 0.075 2220 -2222 2 98.0114 -203.326 64.7298 0.075 2221 -2223 2 90.6732 -177.284 29.2164 0.075 2208 -2224 2 93.6827 -175.514 26.4932 0.075 2223 -2225 2 96.5294 -173.879 23.5211 0.075 2224 -2226 2 96.2576 -177.448 23.587 0.075 2225 -2227 2 96.0425 -180.916 24.369 0.075 2226 -2228 2 96.0328 -183.617 26.2725 0.075 2227 -2229 2 96.2708 -185.356 29.3931 0.075 2228 -2230 2 98.6439 -171.73 22.7195 0.075 2225 -2231 2 100.327 -169.694 21.0593 0.075 2230 -2232 2 102.796 -168.095 21.1194 0.075 2231 -2233 2 105.555 -167.014 22.0028 0.075 2232 -2234 2 108.308 -166.182 23.2028 0.075 2233 -2235 2 110.958 -165.463 24.6461 0.075 2234 -2236 2 113.468 -165.344 26.4951 0.075 2235 -2237 2 116.092 -165.205 25.3688 0.075 2236 -2238 2 116.629 -164.252 22.4469 0.075 2237 -2239 2 118.203 -163.31 19.9431 0.075 2238 -2240 2 120.891 -162.61 18.9186 0.075 2239 -2241 2 123.788 -161.587 18.771 0.075 2240 -2242 2 126.368 -160.295 19.5657 0.075 2241 -2243 2 128.57 -159.123 21.4272 0.075 2242 -2244 2 131.05 -158.278 23.1216 0.075 2243 -2245 2 133.443 -159.911 24.1878 0.075 2244 -2246 2 135.052 -162.315 25.301 0.075 2245 -2247 2 136.246 -165.123 25.753 0.075 2246 -2248 2 137.377 -167.983 26.1442 0.075 2247 -2249 2 138.582 -170.77 26.7333 0.075 2248 -2250 2 139.721 -173.034 26.2499 0.075 2249 -2251 2 140.631 -174.548 23.7022 0.075 2250 -2252 2 141.539 -176.063 21.1544 0.075 2251 -2253 2 142.418 -178.203 19.0908 0.075 2252 -2254 2 143.273 -181.032 18.2983 0.075 2253 -2255 2 144.545 -183.681 17.38 0.075 2254 -2256 2 145.984 -185.962 15.9193 0.075 2255 -2257 2 147.386 -188.032 14.0871 0.075 2256 -2258 2 148.756 -190.149 12.2912 0.075 2257 -2259 2 150.227 -192.443 10.8153 0.075 2258 -2260 2 151.91 -194.743 9.59369 0.075 2259 -2261 2 153.684 -197.001 8.42628 0.075 2260 -2262 2 155.43 -199.155 7.05352 0.075 2261 -2263 2 156.83 -201.537 5.75709 0.075 2262 -2264 2 157.838 -204.333 5.06544 0.075 2263 -2265 2 158.956 -207.211 5.29635 0.075 2264 -2266 2 160.55 -209.839 5.69611 0.075 2265 -2267 2 163.324 -210.936 5.21461 0.075 2266 -2268 2 166.148 -211.879 4.36813 0.075 2267 -2269 2 168.984 -212.933 3.8101 0.075 2268 -2270 2 171.784 -214.092 4.04848 0.075 2269 -2271 2 174.48 -215.277 4.9956 0.075 2270 -2272 2 177.284 -216.494 5.51448 0.075 2271 -2273 2 133.329 -155.963 22.6932 0.075 2244 -2274 2 136.206 -154.434 22.314 0.075 2273 -2275 2 138.721 -152.391 21.8932 0.075 2274 -2276 2 141.278 -150.383 21.4837 0.075 2275 -2277 2 144.088 -148.894 20.9608 0.075 2276 -2278 2 147.05 -147.863 20.2177 0.075 2277 -2279 2 149.4 -145.702 19.4615 0.075 2278 -2280 2 150.321 -144.329 22.0208 0.075 2279 -2281 2 151.718 -142.389 23.9019 0.075 2280 -2282 2 153.206 -140.487 25.7584 0.075 2281 -2283 2 154.931 -138.825 27.6186 0.075 2282 -2284 2 156.68 -137.254 29.5216 0.075 2283 -2285 2 157.904 -135.327 31.5302 0.075 2284 -2286 2 158.328 -133.11 33.578 0.075 2285 -2287 2 158.62 -130.715 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0.075 2718 -2720 2 60.3995 -36.0124 92.7477 0.075 2719 -2721 2 60.7539 -33.7756 94.5973 0.075 2720 -2722 2 60.6251 -30.7168 94.8484 0.075 2721 -2723 2 60.0207 -27.7052 94.954 0.075 2722 -2724 2 59.8699 -24.8672 96.0181 0.075 2723 -2725 2 59.764 -22.0608 97.266 0.075 2724 -2726 2 60.0411 -19.7004 98.9866 0.075 2725 -2727 2 60.892 -17.9624 101.374 0.075 2726 -2728 2 61.7428 -16.2248 103.763 0.075 2727 -2729 2 62.1817 -13.3409 104.685 0.075 2728 -2730 2 62.3992 -10.423 105.622 0.075 2729 -2731 2 63.0377 -7.64869 106.717 0.075 2730 -2732 2 63.7105 -4.93828 107.972 0.075 2731 -2733 2 64.0388 -2.20258 109.331 0.075 2732 -2734 2 64.1857 0.54606 110.698 0.075 2733 -2735 2 64.3895 3.39155 111.797 0.075 2734 -2736 2 65.2584 5.42316 113.902 0.075 2735 -2737 2 66.3908 7.53018 115.795 0.075 2736 -2738 2 67.6429 9.6764 117.575 0.075 2737 -2739 2 68.9197 11.8566 119.295 0.075 2738 -2740 2 70.1324 14.1456 120.874 0.075 2739 -2741 2 70.7069 17.1122 121.337 0.075 2740 -2742 2 71.0385 20.0583 122.066 0.075 2741 -2743 2 71.6587 22.3648 123.747 0.075 2742 -2744 2 72.5806 24.2575 125.96 0.075 2743 -2745 2 73.6459 26.168 128.089 0.075 2744 -2746 2 74.695 28.3502 129.956 0.075 2745 -2747 2 75.4844 31.0956 131.023 0.075 2746 -2748 2 75.7203 33.7609 132.442 0.075 2747 -2749 2 75.3782 36.453 133.778 0.075 2748 -2750 2 74.7899 39.2946 134.621 0.075 2749 -2751 2 74.5933 42.2864 134.959 0.075 2750 -2752 2 75.4116 44.8562 136.398 0.075 2751 -2753 2 76.3912 47.3227 137.914 0.075 2752 -2754 2 77.2717 49.9344 139.221 0.075 2753 -2755 2 77.7904 52.3583 140.871 0.075 2754 -2756 2 77.9665 54.4164 143.123 0.075 2755 -2757 2 78.3061 56.4029 145.419 0.075 2756 -2758 2 77.4912 59.1254 146.546 0.075 2757 -2759 2 76.8406 61.8337 147.801 0.075 2758 -2760 2 76.4006 64.6453 148.887 0.075 2759 -2761 2 76.2194 67.4587 150.006 0.075 2760 -2762 2 76.3273 70.0308 151.64 0.075 2761 -2763 2 76.6093 72.2295 153.746 0.075 2762 -2764 2 77.0604 74.4887 155.754 0.075 2763 -2765 2 77.6333 76.9406 157.469 0.075 2764 -2766 2 77.7574 79.5962 158.848 0.075 2765 -2767 2 77.1235 82.3918 159.861 0.075 2766 -2768 2 76.0843 85.2041 160.458 0.075 2767 -2769 2 76.4317 87.8584 162.045 0.075 2768 -2770 2 76.6278 90.5444 163.605 0.075 2769 -2771 2 76.8306 93.2681 165.096 0.075 2770 -2772 2 77.3502 95.7131 166.91 0.075 2771 -2773 2 77.4887 98.1038 168.82 0.075 2772 -2774 2 77.0915 100.717 170.416 0.075 2773 -2775 2 76.8361 103.578 171.563 0.075 2774 -2776 2 77.3298 106.369 172.85 0.075 2775 -2777 2 77.6859 109.151 174.197 0.075 2776 -2778 2 77.9147 112.131 175.025 0.075 2777 -2779 2 77.9258 115.212 175.297 0.075 2778 -2780 2 77.5683 118.281 175.152 0.075 2779 -2781 2 76.7403 121.249 174.832 0.075 2780 -2782 2 75.5084 124.094 174.617 0.075 2781 -2783 2 74.3606 126.982 174.786 0.075 2782 -2784 2 73.0437 129.723 175.44 0.075 2783 -2785 2 71.7042 132.438 176.16 0.075 2784 -2786 2 70.5333 135.274 176.581 0.075 2785 -2787 2 69.5805 138.219 176.869 0.075 2786 -2788 2 68.7886 141.184 177.352 0.075 2787 -2789 2 68.0696 144.115 178.072 0.075 2788 -2790 2 67.4981 147.174 178.024 0.075 2789 -2791 2 66.6514 150.166 177.931 0.075 2790 -2792 2 65.4608 153.033 177.856 0.075 2791 -2793 2 64.5268 155.978 177.737 0.075 2792 -2794 2 63.8051 159 177.596 0.075 2793 -2795 2 62.9234 161.981 177.554 0.075 2794 -2796 2 61.9444 164.926 177.784 0.075 2795 -2797 2 61.232 167.93 178.17 0.075 2796 -2798 2 60.704 170.846 179.091 0.075 2797 -2799 2 60.5152 173.441 180.72 0.075 2798 -2800 2 60.1732 175.944 182.509 0.075 2799 -2801 2 59.4012 178.622 183.667 0.075 2800 -2802 2 58.3823 181.555 183.754 0.075 2801 -2803 2 57.4995 184.523 183.443 0.075 2802 -2804 2 58.5224 185.382 180.575 0.075 2803 -2805 2 59.5999 186.154 177.702 0.075 2804 -2806 2 60.9554 186.426 174.872 0.075 2805 -2807 2 62.3892 186.557 172.055 0.075 2806 -2808 2 63.9689 186.83 169.345 0.075 2807 -2809 2 65.9483 187.549 167.11 0.075 2808 -2810 2 68.52 189.102 166.117 0.075 2809 -2811 2 71.0831 190.851 165.501 0.075 2810 -2812 2 73.5177 192.819 165.076 0.075 2811 -2813 2 76.1177 194.575 164.839 0.075 2812 -2814 2 78.9796 195.876 164.76 0.075 2813 -2815 2 81.9479 196.743 164.532 0.075 2814 -2816 2 84.4512 197.587 162.791 0.075 2815 -2817 2 86.4811 198.214 160.447 0.075 2816 -2818 2 89.2012 199.335 159.983 0.075 2817 -2819 2 91.3618 200.593 161.736 0.075 2818 -2820 2 93.4056 201.888 163.773 0.075 2819 -2821 2 95.6155 203.44 165.279 0.075 2820 -2822 2 98.0493 205.326 165.949 0.075 2821 -2823 2 100.553 207.257 166.071 0.075 2822 -2824 2 56.0868 186.547 181.571 0.075 2803 -2825 2 54.7195 189.275 180.995 0.075 2824 -2826 2 53.6688 192.175 180.674 0.075 2825 -2827 2 52.4896 194.968 181.104 0.075 2826 -2828 2 51.4405 197.772 181.897 0.075 2827 -2829 2 50.5311 200.645 182.635 0.075 2828 -2830 2 49.4246 203.487 183.199 0.075 2829 -2831 2 48.0246 206.235 183.565 0.075 2830 -2832 2 46.9024 208.895 184.711 0.075 2831 -2833 2 46.3646 211.498 186.284 0.075 2832 -2834 2 46.1657 214.275 187.626 0.075 2833 -2835 2 45.7186 217.102 188.773 0.075 2834 -2836 2 44.7443 219.821 189.892 0.075 2835 -2837 2 43.4459 222.424 190.97 0.075 2836 -2838 2 42.0046 225.074 191.71 0.075 2837 -2839 2 40.5376 227.807 191.561 0.075 2838 -2840 2 39.4071 230.693 191.441 0.075 2839 -2841 2 38.3577 233.604 191.605 0.075 2840 -2842 2 37.079 236.175 192.524 0.075 2841 -2843 2 35.5368 238.337 194.13 0.075 2842 -2844 2 33.9827 240.397 195.855 0.075 2843 -2845 2 32.6274 242.645 197.515 0.075 2844 -2846 2 30.8608 244.973 198.556 0.075 2845 -2847 2 28.8248 247.139 199.439 0.075 2846 -2848 2 26.9576 249.114 200.869 0.075 2847 -2849 2 25.2125 251.069 202.533 0.075 2848 -2850 2 23.401 253.161 203.89 0.075 2849 -2851 2 21.4112 255.416 204.64 0.075 2850 -2852 2 19.2393 257.619 204.92 0.075 2851 -2853 2 17.0767 259.839 204.742 0.075 2852 -2854 2 14.8692 262.018 204.592 0.075 2853 -2855 2 12.6721 264.171 204.863 0.075 2854 -2856 2 11.2355 265.957 206.511 0.075 2855 -2857 2 10.4561 267.426 209.134 0.075 2856 -2858 2 9.67869 268.925 211.74 0.075 2857 -2859 2 8.91167 270.567 214.262 0.075 2858 -2860 2 73.8831 84.4033 162.421 0.075 2768 -2861 2 72.0935 83.8674 164.841 0.075 2860 -2862 2 70.0689 83.0844 166.983 0.075 2861 -2863 2 67.5445 82.3794 168.426 0.075 2862 -2864 2 64.6467 82.1784 169.181 0.075 2863 -2865 2 61.7099 82.7388 169.64 0.075 2864 -2866 2 59.0272 84.0245 170.341 0.075 2865 -2867 2 56.4955 85.4477 171.296 0.075 2866 -2868 2 53.7509 86.5617 172.028 0.075 2867 -2869 2 50.935 87.4996 172.755 0.075 2868 -2870 2 48.0583 88.1447 173.52 0.075 2869 -2871 2 45.1403 88.5033 174.353 0.075 2870 -2872 2 42.3195 88.9311 175.402 0.075 2871 -2873 2 40.1204 89.9024 177.29 0.075 2872 -2874 2 38.046 91.181 179.132 0.075 2873 -2875 2 35.6514 92.5646 180.329 0.075 2874 -2876 2 32.9772 93.8364 181.042 0.075 2875 -2877 2 30.1554 94.9205 181.47 0.075 2876 -2878 2 27.2878 95.9361 181.69 0.075 2877 -2879 2 24.5645 96.8106 182.769 0.075 2878 -2880 2 22.5643 97.5969 184.907 0.075 2879 -2881 2 20.6324 98.3748 187.145 0.075 2880 -2882 2 18.4791 98.9664 189.205 0.075 2881 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63.5087 -64.7194 -26.0502 0.075 3964 -3966 2 64.0824 -66.242 -28.5938 0.075 3965 -3967 2 64.7864 -68.2755 -30.7314 0.075 3966 -3968 2 66.0209 -70.7261 -32.0452 0.075 3967 -3969 2 67.7048 -73.2118 -32.1698 0.075 3968 -3970 2 69.2754 -75.8007 -31.9498 0.075 3969 -3971 2 70.4985 -78.5369 -31.5434 0.075 3970 -3972 2 71.2527 -81.428 -31.0388 0.075 3971 -3973 2 71.5084 -84.4298 -30.615 0.075 3972 -3974 2 86.0372 33.5913 4.55628 0.075 3926 -3975 2 89.4277 34.7896 4.1905 0.075 3974 -3976 2 92.9092 34.778 3.96552 0.075 3975 -3977 2 96.4452 34.0419 3.82483 0.075 3976 -3978 2 90.6672 30.6013 4.63628 0.075 3974 -3979 2 91.9155 34.021 4.1189 0.075 3978 -3980 2 93.6316 37.0925 3.19718 0.075 3979 -3981 2 95.6181 39.9628 2.04166 0.075 3980 -3982 2 92.2828 27.9204 4.77825 0.075 3978 -3983 2 95.2655 26.8162 4.99607 0.075 3982 -3984 2 97.7388 25.9359 6.73263 0.075 3983 -3985 2 100.334 25.0146 8.3249 0.075 3984 -3986 2 103.185 24.5076 9.56819 0.075 3985 -3987 2 106.203 24.2355 10.5393 0.075 3986 -3988 2 109.223 23.6225 11.2612 0.075 3987 -3989 2 112.171 22.4141 11.3598 0.075 3988 -3990 2 115.316 22.0506 11.7062 0.075 3989 -3991 2 118.45 21.8875 12.252 0.075 3990 -3992 2 121.486 21.3027 12.9219 0.075 3991 -3993 2 124.326 20.0858 13.5857 0.075 3992 -3994 2 126.969 18.3854 14.1044 0.075 3993 -3995 2 129.6 16.6088 14.3881 0.075 3994 -3996 2 132.334 14.9958 14.6883 0.075 3995 -3997 2 134.791 12.98 14.8244 0.075 3996 -3998 2 137.378 11.1601 14.5966 0.075 3997 -3999 2 139.918 9.70255 15.3325 0.075 3998 -4000 2 142.354 8.42325 16.943 0.075 3999 -4001 2 144.852 7.20103 18.4838 0.075 4000 -4002 2 147.64 6.24522 19.699 0.075 4001 -4003 2 147.281 3.89844 23.2258 0.075 4002 -4004 2 146.677 0.484291 25.729 0.075 4003 -4005 2 146.547 -3.30379 27.7183 0.075 4004 -4006 2 145.397 -8.55379 29.0383 0.075 4005 -4007 2 147.59 -5.0248 25.1687 0.075 4005 -4008 2 149.375 -7.62535 24.9973 0.075 4007 -4009 2 151.37 -10.1832 25.0914 0.075 4008 -4010 2 153.375 -12.7342 25.18 0.075 4009 -4011 2 155.163 -15.386 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41.2569 0.075 4033 -4035 2 195.961 -4.45762 41.919 0.075 4034 -4036 2 198.948 -4.1726 42.3223 0.075 4035 -4037 2 201.949 -3.78473 42.4026 0.075 4036 -4038 2 204.95 -3.3946 42.4928 0.075 4037 -4039 2 207.96 -3.19347 42.6792 0.075 4038 -4040 2 210.942 -3.40054 43.0018 0.075 4039 -4041 2 213.748 -4.08967 43.6906 0.075 4040 -4042 2 216.01 -4.95273 45.5029 0.075 4041 -4043 2 217.812 -5.95202 47.7207 0.075 4042 -4044 2 219.735 -7.63698 49.295 0.075 4043 -4045 2 221.819 -9.65488 49.9777 0.075 4044 -4046 2 224.109 -11.6067 50.2191 0.075 4045 -4047 2 226.487 -13.422 50.555 0.075 4046 -4048 2 228.683 -15.3084 51.407 0.075 4047 -4049 2 230.349 -17.494 52.6774 0.075 4048 -4050 2 231.36 -19.5757 54.6099 0.075 4049 -4051 2 233.159 -21.5301 55.8914 0.075 4050 -4052 2 235.858 -22.2096 56.0675 0.075 4051 -4053 2 238.863 -22.3103 55.7346 0.075 4052 -4054 2 241.852 -22.5875 55.3573 0.075 4053 -4055 2 244.829 -23.0368 55.0435 0.075 4054 -4056 2 247.846 -23.2164 54.8522 0.075 4055 -4057 2 250.834 -22.842 54.647 0.075 4056 -4058 2 253.833 -22.4957 54.4417 0.075 4057 -4059 2 256.847 -22.3608 54.2442 0.075 4058 -4060 2 259.843 -22.3602 53.9054 0.075 4059 -4061 2 262.761 -22.2479 53.1104 0.075 4060 -4062 2 265.695 -22.4756 52.3975 0.075 4061 -4063 2 268.599 -23.2323 52.5297 0.075 4062 -4064 2 271.338 -24.4028 52.9968 0.075 4063 -4065 2 274.039 -25.3987 52.7101 0.075 4064 -4066 2 276.753 -25.9086 51.4977 0.075 4065 -4067 2 279.491 -26.0848 50.3154 0.075 4066 -4068 2 282.515 -26.1576 50.2126 0.075 4067 -4069 2 284.705 -26.6834 52.2359 0.075 4068 -4070 2 285.827 -27.489 54.8944 0.075 4069 -4071 2 286.824 -28.3275 57.6277 0.075 4070 -4072 2 287.945 -29.5254 60.1309 0.075 4071 -4073 2 289.29 -31.4225 61.9393 0.075 4072 -4074 2 290.764 -33.805 63.0854 0.075 4073 -4075 2 292.173 -36.1511 64.3799 0.075 4074 -4076 2 293.638 -38.4248 65.7408 0.075 4075 -4077 2 295.167 -40.1488 67.6624 0.075 4076 -4078 2 296.67 -41.7245 69.7651 0.075 4077 -4079 2 298.153 -43.3604 71.8343 0.075 4078 -4080 2 299.618 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88.4228 101.487 -11.8713 0.075 4102 -4104 2 88.5118 104.293 -12.9747 0.075 4103 -4105 2 88.5516 106.955 -14.3923 0.075 4104 -4106 2 88.5544 109.565 -15.9168 0.075 4105 -4107 2 88.4651 112.12 -17.5169 0.075 4106 -4108 2 88.2681 114.608 -19.169 0.075 4107 -4109 2 88.764 117.48 -19.9706 0.075 4108 -4110 2 89.6105 120.307 -20.6143 0.075 4109 -4111 2 90.035 123.158 -21.476 0.075 4110 -4112 2 90.2941 126.046 -22.3251 0.075 4111 -4113 2 90.7033 128.947 -23.0303 0.075 4112 -4114 2 91.4648 131.777 -23.7119 0.075 4113 -4115 2 92.5145 134.352 -24.8964 0.075 4114 -4116 2 93.6824 136.848 -26.1387 0.075 4115 -4117 2 94.8536 139.528 -26.8246 0.075 4116 -4118 2 95.9157 142.318 -27.2841 0.075 4117 -4119 2 96.8646 145.147 -27.7589 0.075 4118 -4120 2 97.7673 147.983 -28.2266 0.075 4119 -4121 2 98.6904 150.859 -28.1531 0.075 4120 -4122 2 99.1049 153.63 -27.0194 0.075 4121 -4123 2 99.2754 156.592 -26.5696 0.075 4122 -4124 2 99.3616 159.596 -26.6737 0.075 4123 -4125 2 99.2975 162.504 -27.3625 0.075 4124 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-1699 2 34.0407 -98.1078 26.1862 0.075 1042 -1700 2 37.2483 -99.6838 26.7804 0.075 1699 -1701 2 39.6291 -98.508 29.0184 0.075 1700 -1702 2 41.2267 -96.0096 31.1275 0.075 1701 -1703 2 42.7289 -92.6132 30.8969 0.075 1702 -1704 2 45.9949 -92.017 32.5861 0.075 1703 -1705 2 49.3638 -92.6318 33.9454 0.075 1704 -1706 2 51.43 -95.115 35.6618 0.075 1705 -1707 2 58.4859 -96.5542 36.385 0.075 1706 -1708 2 61.1664 -97.3289 37.7183 0.075 1707 -1709 2 63.545 -99.105 38.4076 0.075 1708 -1710 2 65.3088 -101.624 38.6098 0.075 1709 -1711 2 66.9128 -104.26 38.8361 0.075 1710 -1712 2 68.7418 -106.736 39.1208 0.075 1711 -1713 2 70.5487 -109.209 39.3762 0.075 1712 -1714 2 72.9021 -111.19 39.232 0.075 1713 -1715 2 75.4831 -112.89 39.2331 0.075 1714 -1716 2 77.4789 -115.133 39.9342 0.075 1715 -1717 2 79.1792 -117.668 40.3981 0.075 1716 -1718 2 81.2479 -119.936 40.6397 0.075 1717 -1719 2 83.4687 -122.055 41.0022 0.075 1718 -1720 2 85.5004 -124.091 42.0977 0.075 1719 -1721 2 87.515 -126.114 43.1659 0.075 1720 -1722 2 89.6961 -128.232 43.7386 0.075 1721 -1723 2 91.925 -130.357 44.0311 0.075 1722 -1724 2 94.2161 -132.429 44.1971 0.075 1723 -1725 2 96.5166 -134.496 44.2874 0.075 1724 -1726 2 98.803 -136.579 44.3724 0.075 1725 -1727 2 101.096 -138.643 44.5665 0.075 1726 -1728 2 103.385 -140.605 44.448 0.075 1727 -1729 2 105.443 -142.652 43.3849 0.075 1728 -1730 2 107.574 -144.779 42.7131 0.075 1729 -1731 2 109.779 -146.944 42.5556 0.075 1730 -1732 2 111.974 -149.124 42.5013 0.075 1731 -1733 2 114.129 -151.344 42.4954 0.075 1732 -1734 2 113.916 -152.148 39.8034 0.075 1733 -1735 2 113.329 -152.727 36.827 0.075 1734 -1736 2 111.859 -152.365 34.3275 0.075 1735 -1737 2 109.416 -150.929 33.2788 0.075 1736 -1738 2 58.5833 -97.8939 39.655 0.075 1707 -1739 2 60.3181 -100.059 41.9487 0.075 1738 -1740 2 61.4544 -101.007 45.6625 0.075 1739 -1741 2 62.9312 -101.769 49.3007 0.075 1740 -1742 2 49.4219 -97.1801 34.4588 0.075 1706 -1743 2 47.3063 -98.1884 32.4518 0.075 1742 -1744 2 44.9036 -97.8211 30.5394 0.075 1743 -1745 2 42.2239 -97.2443 29.0861 0.075 1744 -1746 2 39.1795 -97.4751 29.2751 0.075 1745 -1747 2 36.1307 -97.8011 29.9364 0.075 1746 -1748 2 33.0402 -98.0753 30.3418 0.075 1747 -1749 2 30.0163 -98.2968 30.9948 0.075 1748 -1750 2 27.8878 -98.7009 33.1577 0.075 1749 -1751 2 25.6864 -98.9465 35.3536 0.075 1750 -1752 2 22.852 -99.0163 36.2931 0.075 1751 -1753 2 19.773 -99.024 35.7443 0.075 1752 -1754 2 17.0102 -98.7084 34.363 0.075 1753 -1755 2 42.1908 -91.4147 33.7488 0.075 1703 -1756 2 41.8643 -89.9546 36.5065 0.075 1755 -1757 2 41.6 -88.0794 38.9721 0.075 1756 -1758 2 40.9578 -85.4136 40.2736 0.075 1757 -1759 2 40.0778 -82.4175 40.4373 0.075 1758 -1760 2 40.4685 -79.4713 41.1647 0.075 1759 -1761 2 40.9723 -76.5841 42.2566 0.075 1760 -1762 2 40.8685 -73.5355 42.9778 0.075 1761 -1763 2 40.8991 -70.8925 44.4426 0.075 1762 -1764 2 41.7872 -68.3271 45.8972 0.075 1763 -1765 2 42.944 -65.4578 46.3325 0.075 1764 -1766 2 46.1571 -67.0968 47.432 0.075 1765 -1767 2 49.011 -67.8091 49.8815 0.075 1766 -1768 2 52.1302 -69.7038 50.8041 0.075 1767 -1769 2 55.633 -71.061 51.1046 0.075 1768 -1770 2 59.4045 -70.5739 51.1556 0.075 1769 -1771 2 61.9945 -75.8539 52.5689 0.075 1770 -1772 2 61.4209 -70.5561 48.7868 0.075 1770 -1773 2 64.4883 -70.4005 48.3482 0.075 1772 -1774 2 67.5068 -70.9661 48.2022 0.075 1773 -1775 2 70.4159 -72.0353 47.958 0.075 1774 -1776 2 73.3075 -73.0726 47.4805 0.075 1775 -1777 2 75.6448 -74.2828 48.2439 0.075 1776 -1778 2 77.4198 -75.5081 50.451 0.075 1777 -1779 2 79.332 -77.7931 50.9322 0.075 1778 -1780 2 81.0628 -80.1584 51.7618 0.075 1779 -1781 2 83.0263 -81.7566 53.5229 0.075 1780 -1782 2 85.7068 -82.9128 54.2858 0.075 1781 -1783 2 88.4938 -84.2364 54.5381 0.075 1782 -1784 2 90.6259 -86.1943 55.6418 0.075 1783 -1785 2 92.0157 -88.4142 55.4176 0.075 1784 -1786 2 92.5218 -90.1671 52.9308 0.075 1785 -1787 2 93.5186 -92.4575 51.3396 0.075 1786 -1788 2 95.0392 -95.0305 50.5562 0.075 1787 -1789 2 96.709 -97.572 50.0067 0.075 1788 -1790 2 98.736 -99.9263 50.0861 0.075 1789 -1791 2 100.725 -102.317 50.145 0.075 1790 -1792 2 102.612 -104.789 50.1471 0.075 1791 -1793 2 104.171 -107.471 50.2446 0.075 1792 -1794 2 105.438 -110.302 50.3784 0.075 1793 -1795 2 107.006 -112.981 50.274 0.075 1794 -1796 2 108.617 -115.603 50.6588 0.075 1795 -1797 2 110.08 -118.283 51.2498 0.075 1796 -1798 2 112.16 -120.471 50.5681 0.075 1797 -1799 2 114.032 -122.861 49.9531 0.075 1798 -1800 2 115.352 -125.626 50.0202 0.075 1799 -1801 2 116.4 -128.058 51.5348 0.075 1800 -1802 2 42.6492 -63.1157 48.9375 0.075 1765 -1803 2 42.4414 -60.826 51.0506 0.075 1802 -1804 2 41.6783 -57.3372 51.7786 0.075 1803 -1805 2 42.277 -53.8233 52.4106 0.075 1804 -1806 2 42.3228 -50.1683 52.4945 0.075 1805 -1807 2 41.8166 -47.0198 54.0162 0.075 1806 -1808 2 41.2446 -47.0984 50.5052 0.075 1807 -1809 2 40.1903 -46.8428 47.2185 0.075 1808 -1810 2 37.1963 -46.0292 45.9425 0.075 1809 -1811 2 33.8153 -45.4939 45.137 0.075 1810 -1812 2 31.117 -44.7296 43.0291 0.075 1811 -1813 2 29.3165 -43.8004 40.1553 0.075 1812 -1814 2 29.0393 -42.877 36.7847 0.075 1813 -1815 2 29.4968 -42.4661 33.2829 0.075 1814 -1816 2 42.0439 -44.2652 54.5613 0.075 1807 -1817 2 42.0396 -42.4425 57.1642 0.075 1816 -1818 2 42.1399 -40.9047 59.9326 0.075 1817 -1819 2 42.0795 -38.249 61.4325 0.075 1818 -1820 2 41.6655 -35.121 61.5579 0.075 1819 -1821 2 41.7883 -32.3008 62.8412 0.075 1820 -1822 2 42.2882 -29.758 64.6655 0.075 1821 -1823 2 42.6628 -27.8248 67.1557 0.075 1822 -1824 2 42.8433 -25.1815 68.43 0.075 1823 -1825 2 42.3403 -22.0552 68.5766 0.075 1824 -1826 2 42.2544 -19.152 69.8542 0.075 1825 -1827 2 42.5497 -16.7208 71.6438 0.075 1826 -1828 2 43.4296 -14.9236 74.113 0.075 1827 -1829 2 43.9053 -12.0905 75.2669 0.075 1828 -1830 2 44.3481 -9.15296 76.3284 0.075 1829 -1831 2 44.9697 -6.34474 77.6511 0.075 1830 -1832 2 45.1743 -3.50606 79.0623 0.075 1831 -1833 2 45.4077 -0.555615 80.2426 0.075 1832 -1834 2 46.4126 1.58517 82.3833 0.075 1833 -1835 2 47.7175 3.82834 84.2493 0.075 1834 -1836 2 49.0411 6.14337 86.0111 0.075 1835 -1837 2 49.6853 9.16074 86.6677 0.075 1836 -1838 2 50.172 11.8797 87.9899 0.075 1837 -1839 2 51.1759 13.8495 90.2951 0.075 1838 -1840 2 52.2778 16.0522 92.3233 0.075 1839 -1841 2 52.9246 18.916 93.5224 0.075 1840 -1842 2 52.6205 21.7216 94.9527 0.075 1841 -1843 2 52.1645 24.7984 95.4968 0.075 1842 -1844 2 52.9241 27.52 96.8821 0.075 1843 -1845 2 53.9033 30.1732 98.3563 0.075 1844 -1846 2 54.3515 32.6247 100.224 0.075 1845 -1847 2 54.6457 34.7321 102.596 0.075 1846 -1848 2 53.8452 37.5742 103.816 0.075 1847 -1849 2 53.3548 40.4944 104.99 0.075 1848 -1850 2 53.3079 43.3319 106.404 0.075 1849 -1851 2 53.6026 45.6854 108.534 0.075 1850 -1852 2 54.1512 48.1539 110.472 0.075 1851 -1853 2 54.1443 50.96 111.862 0.075 1852 -1854 2 53.1757 53.8959 112.608 0.075 1853 -1855 2 53.5203 56.5122 114.173 0.075 1854 -1856 2 53.7153 59.1692 115.694 0.075 1855 -1857 2 54.1094 61.6866 117.357 0.075 1856 -1858 2 54.1153 64.0655 119.223 0.075 1857 -1859 2 53.7595 66.8337 120.448 0.075 1858 -1860 2 54.2115 69.5826 121.732 0.075 1859 -1861 2 54.4919 72.4347 122.773 0.075 1860 -1862 2 54.4987 75.4734 123.032 0.075 1861 -1863 2 53.8789 78.4505 122.745 0.075 1862 -1864 2 52.6793 81.263 122.601 0.075 1863 -1865 2 51.4613 84.0348 123.015 0.075 1864 -1866 2 50.1423 86.7137 123.72 0.075 1865 -1867 2 49.0991 89.5782 124.003 0.075 1866 -1868 2 48.323 92.4975 124.502 0.075 1867 -1869 2 47.6841 95.4476 124.849 0.075 1868 -1870 2 46.903 98.4103 124.765 0.075 1869 -1871 2 45.7354 101.235 124.685 0.075 1870 -1872 2 45.0315 104.216 124.544 0.075 1871 -1873 2 44.1176 107.138 124.616 0.075 1872 -1874 2 43.3485 110.086 124.957 0.075 1873 -1875 2 42.9246 112.875 126.079 0.075 1874 -1876 2 42.6653 115.33 127.876 0.075 1875 -1877 2 41.804 118.06 128.661 0.075 1876 -1878 2 40.9005 120.977 128.451 0.075 1877 -1879 2 41.9784 121.877 125.435 0.075 1878 -1880 2 43.2139 122.505 122.43 0.075 1879 -1881 2 44.7218 122.643 119.467 0.075 1880 -1882 2 46.5482 123.09 116.739 0.075 1881 -1883 2 49.2436 124.763 115.773 0.075 1882 -1884 2 51.8577 126.742 115.25 0.075 1883 -1885 2 54.6459 128.515 115.036 0.075 1884 -1886 2 57.7333 129.478 114.746 0.075 1885 -1887 2 60.1477 130.265 112.637 0.075 1886 -1888 2 62.8371 131.367 111.993 0.075 1887 -1889 2 64.9698 132.708 114.165 0.075 1888 -1890 2 67.3362 134.407 115.604 0.075 1889 -1891 2 69.9473 136.423 115.903 0.075 1890 -1892 2 39.4692 123.038 126.572 0.075 1878 -1893 2 38.1832 125.857 126.068 0.075 1892 -1894 2 37.039 128.726 126.311 0.075 1893 -1895 2 36.0707 131.601 127.122 0.075 1894 -1896 2 34.9515 134.481 127.697 0.075 1895 -1897 2 33.6497 137.229 128.397 0.075 1896 -1898 2 32.9738 139.88 129.896 0.075 1897 -1899 2 32.7657 142.746 131.144 0.075 1898 -1900 2 31.7719 145.499 132.279 0.075 1899 -1901 2 30.3846 148.161 133.203 0.075 1900 -1902 2 28.9197 150.923 133.223 0.075 1901 -1903 2 27.8492 153.878 133.222 0.075 1902 -1904 2 26.5466 156.474 134.171 0.075 1903 -1905 2 24.9567 158.585 135.878 0.075 1904 -1906 2 23.4933 160.869 137.444 0.075 1905 -1907 2 21.5522 163.145 138.404 0.075 1906 -1908 2 19.6632 165.144 139.862 0.075 1907 -1909 2 17.8679 167.206 141.396 0.075 1908 -1910 2 15.8049 169.478 142.038 0.075 1909 -1911 2 13.6092 171.721 142.042 0.075 1910 -1912 2 11.3718 173.928 141.909 0.075 1911 -1913 2 9.61882 175.88 143.139 0.075 1912 -1914 2 8.82885 177.369 145.798 0.075 1913 -1915 2 8.04833 178.993 148.378 0.075 1914 -1916 2 50.9746 53.0951 114.571 0.075 1854 -1917 2 49.1245 52.4956 116.919 0.075 1916 -1918 2 46.7457 51.7457 118.569 0.075 1917 -1919 2 43.8088 51.7276 119.199 0.075 1918 -1920 2 41.0691 52.8496 119.845 0.075 1919 -1921 2 38.4786 54.1876 120.738 0.075 1920 -1922 2 35.6734 55.1643 121.453 0.075 1921 -1923 2 32.7782 55.7095 122.226 0.075 1922 -1924 2 29.9323 56.1188 123.221 0.075 1923 -1925 2 27.7694 57.178 125.096 0.075 1924 -1926 2 25.4864 58.5422 126.503 0.075 1925 -1927 2 22.7689 59.7575 127.149 0.075 1926 -1928 2 19.9108 60.7881 127.41 0.075 1927 -1929 2 17.408 61.6357 128.811 0.075 1928 -1930 2 15.4764 62.4135 131.049 0.075 1929 -1931 2 13.2487 62.9419 133.049 0.075 1930 -1932 2 10.6809 63.1131 134.677 0.075 1931 -1933 2 7.83983 62.5656 135.617 0.075 1932 -1934 2 4.99035 61.6838 136.286 0.075 1933 -1935 2 2.67164 60.7047 137.875 0.075 1934 -1936 2 1.40069 59.6851 140.453 0.075 1935 -1937 2 -0.208943 58.5669 142.757 0.075 1936 -1938 2 -2.59474 57.3879 144.159 0.075 1937 -1939 2 -5.22811 56.3553 145.312 0.075 1938 -1940 2 -7.89703 55.7531 146.644 0.075 1939 -1941 2 -10.6266 55.7298 147.961 0.075 1940 -1942 2 -12.7605 55.6972 150.109 0.075 1941 -1943 2 -14.5978 55.6449 152.551 0.075 1942 -1944 2 -16.3207 54.8991 154.95 0.075 1943 -1945 2 -18.5091 54.3476 156.931 0.075 1944 -1946 2 -20.6833 54.5503 159.045 0.075 1945 -1947 2 -22.676 54.8288 161.346 0.075 1946 -1948 2 -24.7342 55.1197 163.578 0.075 1947 -1949 2 -26.6275 55.4889 165.837 0.075 1948 -1950 2 -27.801 55.8915 168.631 0.075 1949 -1951 2 -29.4199 56.2742 171.145 0.075 1950 -1952 2 -32.3921 56.4707 171.832 0.075 1951 -1953 2 -35.1921 56.641 172.93 0.075 1952 -1954 2 -37.5766 56.8589 174.816 0.075 1953 -1955 2 -39.7128 57.7904 176.764 0.075 1954 -1956 2 -42.1944 58.8247 177.231 0.075 1955 -1957 2 -43.7318 58.6262 174.853 0.075 1956 -1958 2 -44.5163 57.209 172.355 0.075 1957 -1959 2 42.4084 -1.41038 79.3702 0.075 1832 -1960 2 41.0139 1.71239 78.8273 0.075 1959 -1961 2 40.4872 5.03573 77.7851 0.075 1960 -1962 2 39.6437 8.22912 76.5875 0.075 1961 -1963 2 37.7816 10.6286 74.8891 0.075 1962 -1964 2 35.5535 12.3537 72.7702 0.075 1963 -1965 2 33.2816 12.8243 70.2258 0.075 1964 -1966 2 36.9361 -96.1986 25.9885 0.075 1699 -1967 2 37.7274 -94.1723 23.2093 0.075 1966 -1968 2 38.119 -91.2415 21.5645 0.075 1967 -1969 2 38.5765 -87.7813 20.9448 0.075 1968 -1970 2 39.8919 -84.6866 19.9106 0.075 1969 -1971 2 40.8257 -80.3969 20.1677 0.075 1970 -1972 2 42.066 -76.198 19.652 0.075 1971 -1973 2 42.8726 -73.1179 17.0303 0.075 1972 -1974 2 43.9096 -71.2362 19.6899 0.075 1973 -1975 2 45.2941 -68.486 19.4108 0.075 1974 -1976 2 46.6532 -65.7966 17.7637 0.075 1975 -1977 2 47.8349 -62.6868 18.4259 0.075 1976 -1978 2 49.1991 -59.5453 18.2866 0.075 1977 -1979 2 50.2291 -56.8428 19.7195 0.075 1978 -1980 2 43.7017 -73.058 13.7911 0.075 1973 -1981 2 44.3297 -74.0773 10.6644 0.075 1980 -1982 2 45.7904 -75.2156 7.94613 0.075 1981 -1983 2 48.2999 -76.4457 6.1521 0.075 1982 -1984 2 50.0051 -77.1928 3.4052 0.075 1983 -1985 2 51.1188 -77.5351 0.288908 0.075 1984 -1986 2 52.9496 -76.799 -2.35842 0.075 1985 -1987 2 55.7304 -76.4958 -4.18958 0.075 1986 -1988 2 56.9592 -75.395 -6.6952 0.075 1987 -1989 2 57.3618 -74.3443 -9.82884 0.075 1988 -1990 2 59.3669 -72.9111 -7.82581 0.075 1989 -1991 2 61.7188 -72.0911 -9.74498 0.075 1990 -1992 2 64.268 -71.2057 -11.2742 0.075 1991 -1993 2 67.0766 -69.9647 -10.2741 0.075 1992 -1994 2 69.8577 -68.802 -9.13365 0.075 1993 -1995 2 72.7372 -68.1877 -7.86271 0.075 1994 -1996 2 75.4432 -69.1146 -6.44811 0.075 1995 -1997 2 77.7267 -69.7719 -4.28278 0.075 1996 -1998 2 80.0723 -70.6293 -2.27255 0.075 1997 -1999 2 83.0258 -71.5481 -1.44402 0.075 1998 -2000 2 86.0408 -72.4713 -0.791013 0.075 1999 -2001 2 88.9296 -73.0638 0.482543 0.075 2000 -2002 2 91.5659 -72.8217 2.27568 0.075 2001 -2003 2 93.347 -72.1665 4.88913 0.075 2002 -2004 2 95.4137 -70.9764 6.8752 0.075 2003 -2005 2 97.78 -68.9277 7.58214 0.075 2004 -2006 2 56.3459 -74.7208 -13.4056 0.075 1989 -2007 2 57.5591 -75.6004 -16.9425 0.075 2006 -2008 2 59.2781 -77.3809 -19.7983 0.075 2007 -2009 2 42.9376 -85.5655 19.8893 0.075 1970 -2010 2 46.0891 -85.8857 19.9288 0.075 2009 -2011 2 48.978 -86.8068 20.5068 0.075 2010 -2012 2 51.0713 -88.9163 21.4708 0.075 2011 -2013 2 52.9832 -91.1723 22.6017 0.075 2012 -2014 2 55.001 -92.998 24.1909 0.075 2013 -2015 2 57.4356 -94.6835 25.3212 0.075 2014 -2016 2 59.7293 -96.3378 26.7382 0.075 2015 -2017 2 61.9627 -98.2495 27.909 0.075 2016 -2018 2 64.4149 -99.9856 28.9211 0.075 2017 -2019 2 66.6013 -101.571 30.4687 0.075 2018 -2020 2 68.0345 -102.59 33.0646 0.075 2019 -2021 2 69.8339 -103.523 35.4755 0.075 2020 -2022 2 72.3264 -104.495 37.1485 0.075 2021 -2023 2 75.09 -105.726 37.9913 0.075 2022 -2024 2 77.9171 -106.947 38.7295 0.075 2023 -2025 2 79.2047 -107.593 41.5508 0.075 2024 -2026 2 80.7057 -108.349 44.215 0.075 2025 -2027 2 82.9575 -109.157 46.2417 0.075 2026 -2028 2 85.4844 -109.886 48.013 0.075 2027 -2029 2 26.0599 -89.0992 23.8448 0.0927041 1039 -2030 2 24.9221 -88.8635 26.7245 0.0755338 2029 -2031 2 22.62 -88.2158 28.6635 0.075 2030 -2032 2 19.9011 -86.8788 29.1898 0.075 2031 -2033 2 17.675 -84.6763 29.0946 0.075 2032 -2034 2 15.1711 -82.8059 29.0759 0.075 2033 -2035 2 12.3072 -81.6881 29.6105 0.075 2034 -2036 2 9.76908 -80.6039 31.0609 0.075 2035 -2037 2 7.87192 -78.6409 32.5862 0.075 2036 -2038 2 5.87862 -76.3071 33.0892 0.075 2037 -2039 2 3.88631 -73.8728 32.9385 0.075 2038 -2040 2 1.68333 -71.63 32.8547 0.075 2039 -2041 2 0.930167 -73.544 35.5167 0.075 2040 -2042 2 0.775241 -76.1514 37.5699 0.075 2041 -2043 2 1.52986 -78.8118 39.4989 0.075 2042 -2044 2 2.17404 -81.5134 41.412 0.075 2043 -2045 2 2.10299 -84.0728 43.597 0.075 2044 -2046 2 1.85746 -86.9704 45.2538 0.075 2045 -2047 2 1.81682 -89.9862 46.7489 0.075 2046 -2048 2 1.41225 -93.0202 48.1388 0.075 2047 -2049 2 1.35021 -96.3099 48.7965 0.075 2048 -2050 2 1.88452 -99.4284 49.811 0.075 2049 -2051 2 2.57182 -101.938 51.9196 0.075 2050 -2052 2 -0.00289717 -68.5819 33.8841 0.075 2040 -2053 2 -1.76699 -65.7136 35.1688 0.075 2052 -2054 2 -3.9268 -62.8394 35.7219 0.075 2053 -2055 2 -6.02202 -59.8808 35.9128 0.075 2054 -2056 2 -8.11177 -56.9351 35.6759 0.075 2055 -2057 2 -10.1522 -53.9381 35.4314 0.075 2056 -2058 2 -11.1041 -50.7871 36.1316 0.075 2057 -2059 2 -12.1388 -48.9737 33.6395 0.075 2058 -2060 2 -13.2633 -47.331 30.9919 0.075 2059 -2061 2 -14.8597 -45.0096 29.1852 0.075 2060 -2062 2 -16.5854 -42.3268 28.1888 0.075 2061 -2063 2 -18.6333 -39.6967 27.7819 0.075 2062 -2064 2 -20.8033 -35.1467 25.9286 0.075 2063 -2065 2 -20.7177 -37.2367 28.2837 0.075 2063 -2066 2 -22.9094 -34.8882 28.8581 0.075 2065 -2067 2 -25.1177 -32.5197 29.2123 0.075 2066 -2068 2 -27.3768 -30.1694 29.1618 0.075 2067 -2069 2 -29.2003 -27.5257 29.4534 0.075 2068 -2070 2 -30.7817 -24.7585 30.0145 0.075 2069 -2071 2 -32.3701 -22.6845 28.3589 0.075 2070 -2072 2 -34.2243 -21.242 26.1045 0.075 2071 -2073 2 -36.1898 -19.1261 24.8568 0.075 2072 -2074 2 -37.8638 -16.3665 25.0559 0.075 2073 -2075 2 -39.7944 -13.7662 25.3094 0.075 2074 -2076 2 -41.8657 -11.2679 24.9836 0.075 2075 -2077 2 -43.8198 -8.67682 24.6551 0.075 2076 -2078 2 -45.3706 -5.51582 24.2533 0.075 2077 -2079 2 -47.1003 -2.45003 23.848 0.075 2078 -2080 2 -48.6059 0.10634 22.2013 0.075 2079 -2081 2 -49.755 1.53585 19.218 0.075 2080 -2082 2 -50.9614 3.95103 16.9672 0.075 2081 -2083 2 -52.0838 6.89616 15.365 0.075 2082 -2084 2 -51.4709 11.3939 15.1049 0.075 2083 -2085 2 -55.8999 10.0538 15.5588 0.075 2083 -2086 2 -58.6471 14.2533 15.4512 0.075 2085 -2087 2 -61.2723 15.9901 15.0184 0.075 2086 -2088 2 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2109 -2111 2 -82.0752 73.7675 3.78394 0.075 2110 -2112 2 -85.1341 74.9448 5.22692 0.075 2111 -2113 2 -71.8113 75.189 -8.17452 0.075 2107 -2114 2 -69.3136 76.8092 -6.46413 0.075 2113 -2115 2 -67.2224 78.6473 -4.51137 0.075 2114 -2116 2 -65.3967 80.8506 -5.00749 0.075 2115 -2117 2 -64.3421 83.1572 -7.3145 0.075 2116 -2118 2 -72.6663 78.2633 -8.60888 0.075 2113 -2119 2 -72.3996 81.4394 -8.75341 0.075 2118 -2120 2 -72.3836 84.4496 -8.68316 0.075 2119 -2121 2 -73.9997 86.9743 -9.67957 0.075 2120 -2122 2 -76.13 89.2849 -9.50011 0.075 2121 -2123 2 -77.2506 91.9116 -8.07479 0.075 2122 -2124 2 -78.4288 94.2738 -6.25473 0.075 2123 -2125 2 -80.1349 96.5439 -4.76561 0.075 2124 -2126 2 -81.6078 99.291 -4.37895 0.075 2125 -2127 2 -82.531 102.357 -4.65848 0.075 2126 -2128 2 -81.5809 107.165 -4.42547 0.075 2127 -2129 2 -80.2518 110.605 -7.72515 0.075 2128 -2130 2 -84.7836 105.419 -5.42976 0.075 2127 -2131 2 -85.3267 108.541 -7.98355 0.075 2130 -2132 2 -86.5573 111.923 -9.78343 0.075 2131 -2133 2 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26.3509 13.6388 0.075 2154 -2156 2 -53.3547 29.4144 13.1547 0.075 2155 -2157 2 -52.3921 32.5203 12.995 0.075 2156 -2158 2 -52.1495 35.75 12.8305 0.075 2157 -2159 2 -52.0498 38.8235 11.7964 0.075 2158 -2160 2 -52.3833 41.9437 11.1884 0.075 2159 -2161 2 -52.9182 44.9573 12.1811 0.075 2160 -2162 2 -45.5907 -7.24222 26.93 0.075 2077 -2163 2 -46.5098 -6.02138 29.7628 0.075 2162 -2164 2 -47.5852 -4.72004 32.497 0.075 2163 -2165 2 -48.6845 -3.62758 35.2935 0.075 2164 -2166 2 -50.6145 -2.58513 37.5692 0.075 2165 -2167 2 -53.5437 -1.4214 37.9924 0.075 2166 -2168 2 -56.2506 -0.808691 39.5396 0.075 2167 -2169 2 -58.8206 -0.466484 41.4386 0.075 2168 -2170 2 -61.7015 -0.267606 42.8453 0.075 2169 -2171 2 -64.7997 -0.17169 43.7079 0.075 2170 -2172 2 -67.3006 0.107017 45.3194 0.075 2171 -2173 2 -68.9607 0.282108 48.073 0.075 2172 -2174 2 -70.7765 0.580118 50.7133 0.075 2173 -2175 2 -12.2229 -55.1017 33.2569 0.075 2057 -2176 2 -13.7872 -57.4737 31.8113 0.075 2175 -2177 2 -16.0089 -59.6589 31.6573 0.075 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36.2154 -116.883 28.6498 0.145 1674 -1676 2 38.6451 -120.364 29.0241 0.145 1675 -1677 2 38.8345 -123.646 29.5795 0.110286 1676 -1678 2 39.8124 -126.817 29.8878 0.11 1677 -1679 2 40.5998 -130.046 30.0919 0.11 1678 -1680 2 41.3238 -133.297 30.1496 0.11 1679 -1681 2 42.1238 -136.531 30.0175 0.11 1680 -1682 2 43.0224 -139.815 30.2072 0.104776 1681 -1683 2 44.3561 -142.943 30.4654 0.0827405 1682 -1684 2 46.1171 -145.835 30.6696 0.075 1683 -1685 2 48.1693 -148.549 30.8481 0.075 1684 -1686 2 50.364 -151.153 31.0341 0.075 1685 -1687 2 48.854 -156.953 30.8475 0.075 1686 -1688 2 50.7237 -159.525 30.049 0.075 1687 -1689 2 52.2103 -162.435 30.4053 0.075 1688 -1690 2 54.2538 -164.92 30.9352 0.075 1689 -1691 2 56.1584 -167.514 31.4242 0.075 1690 -1692 2 57.587 -170.447 31.7228 0.075 1691 -1693 2 58.5885 -173.578 31.6788 0.075 1692 -1694 2 59.4211 -176.728 31.271 0.075 1693 -1695 2 60.3747 -179.829 30.7578 0.075 1694 -1696 2 61.4284 -182.908 30.3822 0.075 1695 -1697 2 62.479 -186.022 30.4321 0.075 1696 -1698 2 59.8197 -187.539 27.6869 0.075 1697 -1699 2 56.2505 -187.762 25.5573 0.075 1698 -1700 2 52.943 -187.178 23.0875 0.075 1699 -1701 2 51.2266 -189.213 21.1908 0.075 1700 -1702 2 47.9543 -189.858 20.7315 0.075 1701 -1703 2 44.8931 -188.843 20.1201 0.075 1702 -1704 2 42.2035 -187.515 18.7809 0.075 1703 -1705 2 39.9344 -186.015 16.8492 0.075 1704 -1706 2 38.1241 -183.775 15.1045 0.075 1705 -1707 2 36.3526 -181.606 13.2365 0.075 1706 -1708 2 34.9001 -179.534 11.049 0.075 1707 -1709 2 33.3745 -177.57 8.87601 0.075 1708 -1710 2 30.9813 -175.73 7.38511 0.075 1709 -1711 2 29.5113 -176.73 9.27844 0.075 1710 -1712 2 29.3488 -179.926 11.4977 0.075 1711 -1713 2 28.6585 -183.593 12.5291 0.075 1712 -1714 2 27.4243 -187.159 13.2849 0.075 1713 -1715 2 25.5862 -190.547 13.8417 0.075 1714 -1716 2 23.0334 -193.916 13.8194 0.075 1715 -1717 2 22.0481 -197.78 15.3996 0.075 1716 -1718 2 21.3421 -200.549 16.5473 0.075 1717 -1719 2 21.1585 -203.598 16.9441 0.075 1718 -1720 2 21.2316 -206.609 17.5496 0.075 1719 -1721 2 21.2833 -209.59 18.3005 0.075 1720 -1722 2 21.0813 -212.547 19.1335 0.075 1721 -1723 2 20.4958 -215.467 19.9181 0.075 1722 -1724 2 20.0702 -218.381 20.8138 0.075 1723 -1725 2 19.8095 -221.321 21.6818 0.075 1724 -1726 2 19.729 -224.296 22.4705 0.075 1725 -1727 2 19.9063 -227.244 23.3479 0.075 1726 -1728 2 19.5879 -230.131 24.3396 0.075 1727 -1729 2 18.7655 -232.945 24.5403 0.075 1728 -1730 2 17.5262 -235.567 23.6315 0.075 1729 -1731 2 16.2043 -237.781 21.9473 0.075 1730 -1732 2 14.1427 -239.933 21.2294 0.075 1731 -1733 2 11.6547 -241.641 21.211 0.075 1732 -1734 2 9.0542 -243.016 21.9244 0.075 1733 -1735 2 6.67811 -244.224 23.4263 0.075 1734 -1736 2 5.19742 -245.754 25.6524 0.075 1735 -1737 2 3.86866 -247.888 27.3785 0.075 1736 -1738 2 2.54668 -250.261 28.8203 0.075 1737 -1739 2 1.12734 -252.673 30.099 0.075 1738 -1740 2 -0.59795 -254.904 31.3385 0.075 1739 -1741 2 -2.17999 -257.479 31.7836 0.075 1740 -1742 2 -3.64685 -259.852 30.964 0.075 1741 -1743 2 -4.95515 -262.146 29.4368 0.075 1742 -1744 2 -5.97642 -264.881 28.4547 0.075 1743 -1745 2 -5.86636 -268.398 29.5065 0.075 1744 -1746 2 -6.38961 -272.012 29.741 0.075 1745 -1747 2 -7.31988 -275.529 29.6319 0.075 1746 -1748 2 -8.66424 -278.92 29.2044 0.075 1747 -1749 2 -6.93787 -267.269 30.2724 0.075 1744 -1750 2 -7.70103 -269.66 32.1781 0.075 1749 -1751 2 -8.48569 -272.148 33.9418 0.075 1750 -1752 2 -9.38478 -274.728 35.4953 0.075 1751 -1753 2 -10.468 -277.323 36.8748 0.075 1752 -1754 2 -11.5778 -279.086 39.2397 0.075 1753 -1755 2 -12.6337 -280.219 41.9577 0.075 1754 -1756 2 -13.7519 -280.889 44.8093 0.075 1755 -1757 2 -14.9418 -281.294 47.6955 0.075 1756 -1758 2 -16.0403 -282.04 50.5535 0.075 1757 -1759 2 -17.3724 -283.123 53.1883 0.075 1758 -1760 2 -18.8021 -284.61 55.5358 0.075 1759 -1761 2 -20.1752 -286.624 57.4424 0.075 1760 -1762 2 -21.1677 -289.388 58.5867 0.075 1761 -1763 2 -21.0106 -292.286 59.7571 0.075 1762 -1764 2 -19.8053 -294.834 60.914 0.075 1763 -1765 2 -17.7256 -296.913 61.8761 0.075 1764 -1766 2 -15.0435 -298.473 62.429 0.075 1765 -1767 2 24.1268 -199.617 13.9109 0.075 1717 -1768 2 25.9077 -201.322 11.9466 0.075 1767 -1769 2 27.2875 -203.03 9.69964 0.075 1768 -1770 2 28.9228 -204.746 7.67424 0.075 1769 -1771 2 31.1636 -206.301 6.38282 0.075 1770 -1772 2 34.123 -207.383 6.35872 0.075 1771 -1773 2 36.7681 -208.995 6.94222 0.075 1772 -1774 2 39.2375 -210.902 7.3205 0.075 1773 -1775 2 41.7227 -212.716 7.06416 0.075 1774 -1776 2 44.3585 -214.164 6.17257 0.075 1775 -1777 2 47.2441 -215.047 5.26264 0.075 1776 -1778 2 50.3463 -215.303 4.81671 0.075 1777 -1779 2 53.4207 -215.846 4.45993 0.075 1778 -1780 2 56.334 -216.911 4.17095 0.075 1779 -1781 2 58.9329 -218.629 3.885 0.075 1780 -1782 2 60.8295 -221.024 3.10952 0.075 1781 -1783 2 62.3525 -223.537 1.99007 0.075 1782 -1784 2 63.7656 -225.868 0.449932 0.075 1783 -1785 2 64.9816 -227.944 -1.54511 0.075 1784 -1786 2 65.78 -229.779 -3.97536 0.075 1785 -1787 2 65.8327 -231.718 -6.46038 0.075 1786 -1788 2 66.7792 -233.31 -8.95339 0.075 1787 -1789 2 68.2922 -234.948 -11.1413 0.075 1788 -1790 2 69.9046 -236.768 -13.126 0.075 1789 -1791 2 71.0798 -238.719 -15.3049 0.075 1790 -1792 2 22.5545 -189.289 14.2224 0.075 1715 -1793 2 19.3283 -189.205 14.9889 0.075 1792 -1794 2 16.1243 -189.104 15.8391 0.075 1793 -1795 2 12.9207 -189.148 16.6247 0.075 1794 -1796 2 9.67693 -189.704 17.0385 0.075 1795 -1797 2 6.5166 -190.375 17.772 0.075 1796 -1798 2 3.39887 -191.161 18.5793 0.075 1797 -1799 2 0.316611 -192.081 19.3898 0.075 1798 -1800 2 29.305 -175.402 6.41648 0.075 1711 -1801 2 27.7693 -174.388 3.85191 0.075 1800 -1802 2 25.4837 -173.811 1.82041 0.075 1801 -1803 2 23.1678 -173.562 -0.280489 0.075 1802 -1804 2 21.2202 -173.559 -2.74433 0.075 1803 -1805 2 20.003 -173.655 -5.66093 0.075 1804 -1806 2 18.7858 -173.75 -8.57755 0.075 1805 -1807 2 17.5978 -173.686 -11.5027 0.075 1806 -1808 2 16.5049 -173.468 -14.4582 0.075 1807 -1809 2 15.688 -173.257 -17.5054 0.075 1808 -1810 2 14.9995 -173.282 -20.5897 0.075 1809 -1811 2 14.3281 -173.339 -23.6789 0.075 1810 -1812 2 13.444 -172.961 -26.5336 0.075 1811 -1813 2 11.8812 -171.195 -28.6402 0.075 1812 -1814 2 28.1113 -171.34 6.02511 0.075 1710 -1815 2 26.3617 -172.815 3.85629 0.075 1814 -1816 2 24.1372 -174.196 2.10181 0.075 1815 -1817 2 21.7674 -175.672 0.658063 0.075 1816 -1818 2 19.4383 -177.259 0.516921 0.075 1817 -1819 2 17.2781 -178.984 1.9832 0.075 1818 -1820 2 15.1334 -181.279 2.2508 0.075 1819 -1821 2 13.632 -183.142 0.269055 0.075 1820 -1822 2 12.1905 -185.148 -1.68284 0.075 1821 -1823 2 10.9545 -187.401 -3.4726 0.075 1822 -1824 2 10.1293 -189.973 -5.09802 0.075 1823 -1825 2 9.45853 -192.633 -6.6528 0.075 1824 -1826 2 8.95574 -195.603 -7.13409 0.075 1825 -1827 2 8.40218 -198.7 -7.21572 0.075 1826 -1828 2 7.5837 -200.878 -9.18583 0.075 1827 -1829 2 6.67966 -203.304 -10.8551 0.075 1828 -1830 2 5.64504 -206.125 -11.6177 0.075 1829 -1831 2 4.4537 -209.044 -11.5631 0.075 1830 -1832 2 26.3104 -168.317 4.98304 0.075 1814 -1833 2 23.9389 -165.562 4.45211 0.075 1832 -1834 2 21.7162 -162.658 4.15953 0.075 1833 -1835 2 19.7184 -159.593 3.90867 0.075 1834 -1836 2 17.7583 -156.51 3.65175 0.075 1835 -1837 2 15.3929 -153.707 3.4549 0.075 1836 -1838 2 13.5653 -154.077 0.375744 0.075 1837 -1839 2 11.066 -155.582 -1.71561 0.075 1838 -1840 2 8.74006 -157.934 -2.28332 0.075 1839 -1841 2 6.46583 -160.698 -1.92626 0.075 1840 -1842 2 4.07373 -163.343 -1.43821 0.075 1841 -1843 2 2.68771 -161.223 0.469219 0.075 1842 -1844 2 1.37474 -158.431 1.23624 0.075 1843 -1845 2 -0.338424 -155.794 1.49913 0.075 1844 -1846 2 -1.68847 -153.781 2.95794 0.075 1845 -1847 2 -2.45591 -152.223 5.60595 0.075 1846 -1848 2 -2.77431 -150.552 8.29056 0.075 1847 -1849 2 -2.83489 -148.112 10.3182 0.075 1848 -1850 2 -2.97285 -145.442 12.0167 0.075 1849 -1851 2 -3.352 -142.739 13.6236 0.075 1850 -1852 2 -4.10282 -140.202 15.3629 0.075 1851 -1853 2 -5.31636 -138.161 17.4756 0.075 1852 -1854 2 -6.915 -136.047 19.2119 0.075 1853 -1855 2 -8.69338 -133.903 20.7357 0.075 1854 -1856 2 -10.3415 -131.867 22.4298 0.075 1855 -1857 2 -11.413 -130.169 24.8874 0.075 1856 -1858 2 -12.089 -129.726 27.9614 0.075 1857 -1859 2 -13.1772 -129.233 30.8957 0.075 1858 -1860 2 -14.4053 -128.723 33.7825 0.075 1859 -1861 2 -15.9329 -128.244 36.341 0.075 1860 -1862 2 -18.9597 -127.921 37.2555 0.075 1861 -1863 2 3.50266 -165.329 -4.56848 0.075 1842 -1864 2 2.59631 -165.276 -8.14513 0.075 1863 -1865 2 1.26557 -164.426 -11.473 0.075 1864 -1866 2 -0.475399 -163.51 -14.4957 0.075 1865 -1867 2 -2.62923 -164.915 -17.2263 0.075 1866 -1868 2 14.7129 -146.957 2.5749 0.075 1837 -1869 2 16.8619 -146.153 0.05149 0.075 1868 -1870 2 18.4777 -145.852 -2.93131 0.075 1869 -1871 2 20.9504 -145.1 -3.58642 0.075 1870 -1872 2 24.0983 -144.594 -2.72407 0.075 1871 -1873 2 27.3788 -145.284 -2.09588 0.075 1872 -1874 2 29.6391 -147.571 -1.88221 0.075 1873 -1875 2 32.3306 -149.008 -0.834188 0.075 1874 -1876 2 35.0505 -150.332 0.282564 0.075 1875 -1877 2 37.9136 -151.539 0.900185 0.075 1876 -1878 2 40.9634 -152.477 0.942829 0.075 1877 -1879 2 44.151 -152.351 1.41993 0.075 1878 -1880 2 46.8189 -152.895 3.04469 0.075 1879 -1881 2 49.1879 -153.707 5.06135 0.075 1880 -1882 2 51.3955 -154.627 7.22504 0.075 1881 -1883 2 53.5414 -155.574 9.43941 0.075 1882 -1884 2 28.4984 -144.106 -4.80657 0.075 1873 -1885 2 30.6443 -143.488 -7.0448 0.075 1884 -1886 2 32.3705 -142.41 -9.43147 0.075 1885 -1887 2 34.2584 -141.225 -11.6259 0.075 1886 -1888 2 36.6236 -140.115 -13.3678 0.075 1887 -1889 2 39.3776 -138.944 -14.3882 0.075 1888 -1890 2 41.8749 -137.897 -16.0032 0.075 1889 -1891 2 44.4131 -137.433 -17.7707 0.075 1890 -1892 2 46.8147 -137.543 -19.7677 0.075 1891 -1893 2 48.8541 -138.051 -22.1166 0.075 1892 -1894 2 51.4941 -137.706 -23.8221 0.075 1893 -1895 2 54.4982 -137.419 -23.9504 0.075 1894 -1896 2 57.5733 -137.136 -23.3178 0.075 1895 -1897 2 60.3088 -136.986 -23.2513 0.075 1896 -1898 2 62.1077 -137.316 -25.8305 0.075 1897 -1899 2 13.4129 -145.027 2.32156 0.075 1868 -1900 2 12.7719 -145.366 5.60999 0.075 1899 -1901 2 11.3349 -145.886 8.60754 0.075 1900 -1902 2 9.99348 -146.69 11.5813 0.075 1901 -1903 2 8.73326 -147.652 14.552 0.075 1902 -1904 2 7.51292 -148.628 17.535 0.075 1903 -1905 2 11.8722 -141.008 1.90801 0.075 1899 -1906 2 10.2867 -137.059 1.82874 0.075 1905 -1907 2 9.06642 -133.679 4.23428 0.075 1906 -1908 2 7.56816 -133.813 1.58063 0.075 1907 -1909 2 6.06992 -133.948 -1.07296 0.075 1908 -1910 2 4.29431 -134.487 -3.45796 0.075 1909 -1911 2 2.31974 -135.479 -5.51756 0.075 1910 -1912 2 0.244639 -136.949 -7.19833 0.075 1911 -1913 2 -2.43249 -138.197 -7.95978 0.075 1912 -1914 2 -4.87501 -139.934 -8.32318 0.075 1913 -1915 2 -6.81402 -141.908 -9.20686 0.075 1914 -1916 2 -8.18485 -143.93 -11.0322 0.075 1915 -1917 2 -9.41576 -145.99 -12.9161 0.075 1916 -1918 2 -11.0376 -148.208 -14.1718 0.075 1917 -1919 2 -13.0423 -150.3 -15.0271 0.075 1918 -1920 2 -15.3049 -152.197 -15.7127 0.075 1919 -1921 2 -16.9951 -154.309 -17.1224 0.075 1920 -1922 2 -18.1641 -156.914 -18.1123 0.075 1921 -1923 2 -19.5452 -159.483 -18.1709 0.075 1922 -1924 2 -21.27 -161.84 -17.33 0.075 1923 -1925 2 -23.059 -163.94 -16.0276 0.075 1924 -1926 2 -25.3579 -165.808 -15.3859 0.075 1925 -1927 2 -27.9998 -167.193 -14.9766 0.075 1926 -1928 2 -30.8889 -168.1 -14.6694 0.075 1927 -1929 2 -33.8738 -168.636 -14.3424 0.075 1928 -1930 2 -36.8178 -169.319 -13.9332 0.075 1929 -1931 2 -39.7284 -169.834 -13.2413 0.075 1930 -1932 2 -42.5717 -170.269 -12.8545 0.075 1931 -1933 2 -45.2758 -170.925 -14.1042 0.075 1932 -1934 2 -47.7478 -171.822 -15.6462 0.075 1933 -1935 2 -50.5329 -172.599 -16.3163 0.075 1934 -1936 2 -53.5183 -173.155 -16.379 0.075 1935 -1937 2 -56.552 -173.405 -16.1832 0.075 1936 -1938 2 8.23367 -129.616 3.79697 0.075 1907 -1939 2 6.65799 -125.936 2.49874 0.075 1938 -1940 2 4.76537 -122.212 1.92597 0.075 1939 -1941 2 3.0008 -122.648 -0.539938 0.075 1940 -1942 2 0.34706 -122.203 -2.00682 0.075 1941 -1943 2 -2.04161 -121.554 -3.78562 0.075 1942 -1944 2 -3.99948 -120.733 -5.93772 0.075 1943 -1945 2 -5.53378 -119.761 -8.34757 0.075 1944 -1946 2 -7.35533 -119.496 -7.36591 0.075 1945 -1947 2 -8.43557 -120.37 -5.55017 0.075 1946 -1948 2 -9.54459 -122.18 -7.74426 0.075 1947 -1949 2 -10.9751 -124.43 -9.25472 0.075 1948 -1950 2 -12.5867 -126.91 -9.90956 0.075 1949 -1951 2 -14.0791 -129.53 -10.4178 0.075 1950 -1952 2 -15.3853 -131.792 -11.6254 0.075 1951 -1953 2 -16.6817 -133.203 -14.0143 0.075 1952 -1954 2 -18.5747 -134.695 -15.907 0.075 1953 -1955 2 -20.603 -135.914 -14.7863 0.075 1954 -1956 2 -21.7649 -136.996 -12.379 0.075 1955 -1957 2 -22.1225 -138.013 -9.52873 0.075 1956 -1958 2 -23.1871 -139.032 -6.84182 0.075 1957 -1959 2 -25.7618 -139.82 -5.61635 0.075 1958 -1960 2 -28.5329 -140.884 -4.97951 0.075 1959 -1961 2 -31.2731 -142.207 -4.62423 0.075 1960 -1962 2 -33.964 -143.63 -4.27011 0.075 1961 -1963 2 -36.7131 -144.948 -3.95919 0.075 1962 -1964 2 -39.4334 -146.319 -3.63001 0.075 1963 -1965 2 -42.1273 -147.693 -3.17258 0.075 1964 -1966 2 -44.8102 -148.968 -2.41893 0.075 1965 -1967 2 -47.5969 -149.943 -1.60074 0.075 1966 -1968 2 -50.4316 -150.829 -0.850338 0.075 1967 -1969 2 -53.318 -151.372 -0.100428 0.075 1968 -1970 2 -56.2625 -151.253 0.740504 0.075 1969 -1971 2 4.69464 -119.684 3.83561 0.075 1940 -1972 2 3.9307 -116.635 3.78477 0.075 1971 -1973 2 2.92834 -113.646 3.52668 0.075 1972 -1974 2 1.65294 -110.831 3.62198 0.075 1973 -1975 2 0.0588027 -108.298 4.64679 0.075 1974 -1976 2 -0.51841 -105.433 5.87365 0.075 1975 -1977 2 -1.25532 -102.456 6.57755 0.075 1976 -1978 2 -2.28753 -99.5026 6.75936 0.075 1977 -1979 2 -3.64092 -96.6576 6.47057 0.075 1978 -1980 2 -4.1735 -93.5577 6.08214 0.075 1979 -1981 2 -4.32471 -90.4267 5.68883 0.075 1980 -1982 2 -4.4564 -87.2896 5.30394 0.075 1981 -1983 2 -4.85481 -84.1674 4.93872 0.075 1982 -1984 2 -5.7318 -81.1389 4.61829 0.075 1983 -1985 2 -6.63917 -78.119 4.30186 0.075 1984 -1986 2 -7.30141 -75.0617 3.96185 0.075 1985 -1987 2 -7.4327 -71.9196 3.56965 0.075 1986 -1988 2 -8.87995 -68.5208 3.30319 0.075 1987 -1989 2 -10.8877 -65.447 3.34096 0.075 1988 -1990 2 -13.1536 -62.5227 3.5188 0.075 1989 -1991 2 -14.9436 -61.9914 5.96003 0.075 1990 -1992 2 -16.6391 -62.2946 8.50157 0.075 1991 -1993 2 -17.974 -62.9474 11.1669 0.075 1992 -1994 2 -18.2344 -64.0362 13.7608 0.075 1993 -1995 2 -16.9954 -65.5953 16.0936 0.075 1994 -1996 2 -15.6444 -67.7823 17.7783 0.075 1995 -1997 2 -14.2877 -70.0227 19.3853 0.075 1996 -1998 2 -13.21 -71.8953 21.4217 0.075 1997 -1999 2 -12.5535 -73.1876 24.1295 0.075 1998 -2000 2 -12.1081 -74.2059 26.9949 0.075 1999 -2001 2 -11.5845 -75.3489 29.7979 0.075 2000 -2002 2 -11.0275 -76.6298 32.5327 0.075 2001 -2003 2 -10.5173 -78.1213 35.1465 0.075 2002 -2004 2 -10.5982 -80.4256 37.1787 0.075 2003 -2005 2 -10.9651 -82.6869 39.2218 0.075 2004 -2006 2 -11.5932 -85.113 40.9407 0.075 2005 -2007 2 -12.4675 -87.7 42.3333 0.075 2006 -2008 2 -13.3769 -89.8111 44.2318 0.075 2007 -2009 2 -13.458 -90.0471 47.2951 0.075 2008 -2010 2 -13.5391 -90.2832 50.3584 0.075 2009 -2011 2 -13.6202 -90.5193 53.4217 0.075 2010 -2012 2 -13.6865 -90.8239 56.4702 0.075 2011 -2013 2 -13.5931 -91.8644 59.3606 0.075 2012 -2014 2 -13.6529 -94.4909 60.6735 0.075 2013 -2015 2 -14.8147 -97.1108 61.0872 0.075 2014 -2016 2 -16.6753 -99.5048 60.8212 0.075 2015 -2017 2 -18.543 -101.764 59.8971 0.075 2016 -2018 2 -19.1923 -104.651 58.7672 0.075 2017 -2019 2 -19.5351 -106.837 56.5018 0.075 2018 -2020 2 -19.7836 -109.058 54.2582 0.075 2019 -2021 2 -19.8543 -111.163 51.9132 0.075 2020 -2022 2 -19.6546 -112.988 49.3324 0.075 2021 -2023 2 -19.0982 -114.159 46.4423 0.075 2022 -2024 2 -18.6034 -115.254 43.5124 0.075 2023 -2025 2 -18.1262 -116.339 40.5755 0.075 2024 -2026 2 -17.6234 -117.483 37.6646 0.075 2025 -2027 2 -16.9762 -119.314 35.163 0.075 2026 -2028 2 -16.2144 -121.076 32.6449 0.075 2027 -2029 2 -15.1668 -122.862 30.2843 0.075 2028 -2030 2 -13.6416 -124.777 28.2746 0.075 2029 -2031 2 -18.7577 -102.187 63.011 0.075 2017 -2032 2 -19.5987 -103.722 65.629 0.075 2031 -2033 2 -20.6421 -105.853 67.6671 0.075 2032 -2034 2 -21.0405 -107.425 70.1994 0.075 2033 -2035 2 -21.0585 -108.426 71.3111 0.075 2034 -2036 2 -21.2512 -109.001 68.2251 0.075 2035 -2037 2 -21.2672 -109.598 65.1752 0.075 2036 -2038 2 -20.6946 -109.808 67.8118 0.075 2037 -2039 2 -20.1263 -109.919 70.9079 0.075 2038 -2040 2 -19.558 -110.03 74.0041 0.075 2039 -2041 2 -18.9808 -110.157 77.0941 0.075 2040 -2042 2 -17.7914 -111.319 79.7691 0.075 2041 -2043 2 -16.9238 -112.52 82.5425 0.075 2042 -2044 2 -16.7484 -113.389 85.4736 0.075 2043 -2045 2 -17.3404 -113.904 88.5149 0.075 2044 -2046 2 -18.3944 -114.552 91.4116 0.075 2045 -2047 2 -19.6463 -116.153 93.7839 0.075 2046 -2048 2 -20.7831 -118.652 94.8936 0.075 2047 -2049 2 -21.7278 -121.621 95.3282 0.075 2048 -2050 2 -22.4476 -124.656 95.7612 0.075 2049 -2051 2 -22.9863 -127.72 96.2557 0.075 2050 -2052 2 -23.6315 -130.68 97.0895 0.075 2051 -2053 2 -24.1886 -133.549 98.2335 0.075 2052 -2054 2 -24.4645 -136.25 99.7651 0.075 2053 -2055 2 -24.1852 -138.488 101.963 0.075 2054 -2056 2 -23.8974 -140.736 104.151 0.075 2055 -2057 2 -23.3719 -143.162 106.058 0.075 2056 -2058 2 -22.4819 -145.79 107.431 0.075 2057 -2059 2 -21.1565 -148.58 108.049 0.075 2058 -2060 2 -13.9568 -59.3177 2.78303 0.075 1990 -2061 2 -14.5951 -56.1622 1.74102 0.075 2060 -2062 2 -15.4385 -53.0361 0.77858 0.075 2061 -2063 2 -16.5034 -49.9309 -0.0226232 0.075 2062 -2064 2 -17.7859 -46.8214 -0.403058 0.075 2063 -2065 2 -18.8278 -43.6202 -0.725346 0.075 2064 -2066 2 -19.5632 -40.3396 -0.960536 0.075 2065 -2067 2 -19.9169 -36.9752 -1.08201 0.075 2066 -2068 2 -21.0525 -34.0703 -1.22482 0.075 2067 -2069 2 -21.5829 -31.0655 -1.89773 0.075 2068 -2070 2 -21.87 -28.0267 -2.5484 0.075 2069 -2071 2 -21.8346 -24.951 -2.88289 0.075 2070 -2072 2 -21.5588 -21.8539 -2.72697 0.075 2071 -2073 2 -21.5792 -18.8087 -2.02778 0.075 2072 -2074 2 -22.3599 -15.8098 -1.86119 0.075 2073 -2075 2 -23.6131 -12.9976 -2.15944 0.075 2074 -2076 2 -24.9932 -10.2567 -2.72499 0.075 2075 -2077 2 -26.128 -7.44698 -3.43621 0.075 2076 -2078 2 -27.0093 -4.76642 -4.77817 0.075 2077 -2079 2 -27.7689 -1.82987 -5.46165 0.075 2078 -2080 2 -28.73 1.08367 -5.36664 0.075 2079 -2081 2 -29.7239 4.00124 -5.01672 0.075 2080 -2082 2 -30.4291 7.03572 -5.17425 0.075 2081 -2083 2 -31.1007 9.87391 -6.29519 0.075 2082 -2084 2 -31.9205 12.6883 -7.37296 0.075 2083 -2085 2 -32.8376 15.5426 -8.21478 0.075 2084 -2086 2 -33.81 18.4616 -8.75569 0.075 2085 -2087 2 -34.7782 21.4067 -9.14538 0.075 2086 -2088 2 -35.6522 24.3716 -9.59958 0.075 2087 -2089 2 -36.6281 27.2919 -10.1175 0.075 2088 -2090 2 -37.8485 30.1127 -10.5975 0.075 2089 -2091 2 -39.4566 32.7757 -10.8888 0.075 2090 -2092 2 -42.1416 33.634 -14.3836 0.075 2091 -2093 2 -45.1251 34.7811 -17.5426 0.075 2092 -2094 2 -46.2887 37.6099 -16.6812 0.075 2093 -2095 2 -47.8262 40.273 -17.5358 0.075 2094 -2096 2 -48.9053 42.7455 -19.1587 0.075 2095 -2097 2 -49.8921 45.3451 -20.6454 0.075 2096 -2098 2 -50.9467 48.209 -21.5573 0.075 2097 -2099 2 -51.9572 51.2067 -21.9806 0.075 2098 -2100 2 -52.8159 54.2519 -22.3922 0.075 2099 -2101 2 -53.5209 57.3376 -22.7823 0.075 2100 -2102 2 -54.0963 60.4551 -23.1513 0.075 2101 -2103 2 -54.6441 63.5785 -23.5123 0.075 2102 -2104 2 -55.3581 66.6084 -24.1979 0.075 2103 -2105 2 -56.0059 69.4325 -25.435 0.075 2104 -2106 2 -56.6155 72.0364 -27.1691 0.075 2105 -2107 2 -57.4116 74.462 -29.0844 0.075 2106 -2108 2 -58.4154 77.0778 -30.6035 0.075 2107 -2109 2 -59.6185 79.7355 -31.8774 0.075 2108 -2110 2 -60.9839 82.4203 -32.9219 0.075 2109 -2111 2 -62.4414 85.1449 -33.721 0.075 2110 -2112 2 -64.0541 87.7712 -34.5488 0.075 2111 -2113 2 -65.7848 90.1522 -35.7332 0.075 2112 -2114 2 -67.5856 92.3698 -37.1558 0.075 2113 -2115 2 -69.3978 94.5475 -38.6254 0.075 2114 -2116 2 -47.5236 34.5607 -19.6851 0.075 2093 -2117 2 -49.4332 35.4508 -22.2124 0.075 2116 -2118 2 -51.5752 35.8317 -24.6601 0.075 2117 -2119 2 -53.9748 35.7953 -26.8251 0.075 2118 -2120 2 -56.7949 35.1731 -28.4017 0.075 2119 -2121 2 -59.5954 34.4846 -29.9853 0.075 2120 -2122 2 -62.0413 34.5557 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2210 -2212 2 -81.3817 177.735 -44.9967 0.075 2211 -2213 2 -82.2256 180.432 -46.2608 0.075 2212 -2214 2 -82.8531 183.16 -47.607 0.075 2213 -2215 2 -83.4212 185.951 -48.8483 0.075 2214 -2216 2 -19.0441 -35.0289 1.37899 0.075 2067 -2217 2 -19.5416 -31.78 1.00586 0.075 2216 -2218 2 -19.4997 -28.5082 0.681591 0.075 2217 -2219 2 -19.1121 -25.2353 0.465016 0.075 2218 -2220 2 -18.5387 -21.9787 0.412374 0.075 2219 -2221 2 -18.3489 -18.711 0.891025 0.075 2220 -2222 2 -17.6939 -15.4967 1.04293 0.075 2221 -2223 2 -16.6889 -12.3621 0.879119 0.075 2222 -2224 2 -15.4609 -9.32681 0.42028 0.075 2223 -2225 2 -15.4165 -6.06068 -0.102356 0.075 2224 -2226 2 -15.8371 -2.8535 -0.712576 0.075 2225 -2227 2 -16.3082 0.336803 -1.40779 0.075 2226 -2228 2 -16.4519 3.54837 -2.18747 0.075 2227 -2229 2 -18.9522 4.45885 -3.738 0.075 2228 -2230 2 -21.9702 4.32501 -4.44969 0.075 2229 -2231 2 -24.6017 4.56797 -5.98935 0.075 2230 -2232 2 -27.0561 5.14319 -7.78249 0.075 2231 -2233 2 -29.4357 6.05854 -9.53545 0.075 2232 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22.1757 -14.2217 0.075 2255 -2257 2 -9.50478 25.083 -14.9136 0.075 2256 -2258 2 -8.55375 27.8985 -15.7032 0.075 2257 -2259 2 -7.22482 30.5277 -16.5814 0.075 2258 -2260 2 -6.4489 32.9562 -15.5352 0.075 2259 -2261 2 -5.81083 35.2816 -13.6337 0.075 2260 -2262 2 -4.90972 37.6222 -11.9085 0.075 2261 -2263 2 -3.4695 40.2535 -11.2325 0.075 2262 -2264 2 -2.02525 42.9114 -11.5119 0.075 2263 -2265 2 -0.849055 45.6634 -12.1467 0.075 2264 -2266 2 -0.0671178 48.543 -12.6894 0.075 2265 -2267 2 0.205277 51.5859 -12.3413 0.075 2266 -2268 2 0.185516 53.8549 -10.3325 0.075 2267 -2269 2 0.408799 55.8187 -7.99712 0.075 2268 -2270 2 0.781291 57.6457 -5.55203 0.075 2269 -2271 2 1.16036 59.4885 -3.13378 0.075 2270 -2272 2 1.97963 62.3855 -2.50817 0.075 2271 -2273 2 2.88029 65.2983 -2.52575 0.075 2272 -2274 2 4.15448 68.0458 -2.79854 0.075 2273 -2275 2 5.96499 70.5097 -3.06175 0.075 2274 -2276 2 8.15163 72.6708 -3.12269 0.075 2275 -2277 2 10.259 74.9061 -3.24073 0.075 2276 -2278 2 12.2565 77.1516 -3.7575 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321.024 -46.8337 77.5428 0.075 4413 -4415 2 322.482 -48.7906 79.2841 0.075 4414 -4416 2 323.831 -51.2688 80.4213 0.075 4415 -4417 2 84.2412 44.3478 3.33583 0.075 4247 -4418 2 85.1104 48.4491 1.53492 0.075 4417 -4419 2 84.3897 51.3014 2.23872 0.075 4418 -4420 2 84.5812 54.2604 2.84868 0.075 4419 -4421 2 84.4942 57.226 2.62034 0.075 4420 -4422 2 84.6172 59.9938 1.58769 0.075 4421 -4423 2 85.2426 62.529 0.0897999 0.075 4422 -4424 2 86.4361 65.058 -1.06937 0.075 4423 -4425 2 86.8509 67.9737 -1.67472 0.075 4424 -4426 2 87.5865 70.8241 -2.2863 0.075 4425 -4427 2 88.6689 73.5755 -2.92458 0.075 4426 -4428 2 89.9147 76.2703 -3.51441 0.075 4427 -4429 2 90.7702 79.1485 -3.89954 0.075 4428 -4430 2 91.6322 82.0232 -4.29572 0.075 4429 -4431 2 92.453 84.8078 -5.04248 0.075 4430 -4432 2 93.1576 87.4771 -6.27529 0.075 4431 -4433 2 93.4788 90.1967 -7.56553 0.075 4432 -4434 2 93.6963 93.0259 -8.61207 0.075 4433 -4435 2 94.0893 95.8531 -9.60275 0.075 4434 -4436 2 94.6055 98.6838 -10.5316 0.075 4435 -4437 2 94.9506 101.598 -11.2729 0.075 4436 -4438 2 95.2262 104.511 -12.0517 0.075 4437 -4439 2 95.1189 107.425 -12.8119 0.075 4438 -4440 2 94.9526 110.301 -13.6954 0.075 4439 -4441 2 95.0791 113.088 -14.8647 0.075 4440 -4442 2 95.0818 115.702 -16.3915 0.075 4441 -4443 2 95.0846 118.316 -17.9186 0.075 4442 -4444 2 94.9519 120.85 -19.5569 0.075 4443 -4445 2 94.7943 123.364 -21.1626 0.075 4444 -4446 2 95.291 126.24 -21.9655 0.075 4445 -4447 2 96.122 129.074 -22.618 0.075 4446 -4448 2 96.6332 131.924 -23.4369 0.075 4447 -4449 2 96.8971 134.804 -24.3285 0.075 4448 -4450 2 97.1531 137.72 -25.0986 0.075 4449 -4451 2 97.8588 140.605 -25.6821 0.075 4450 -4452 2 98.8165 143.267 -26.7051 0.075 4451 -4453 2 99.9207 145.811 -27.9174 0.075 4452 -4454 2 101.095 148.339 -29.0679 0.075 4453 -4455 2 102.268 151.082 -29.5803 0.075 4454 -4456 2 103.279 153.901 -30.0153 0.075 4455 -4457 2 104.215 156.734 -30.5175 0.075 4456 -4458 2 105.121 159.579 -30.9379 0.075 4457 -4459 2 106.047 162.46 -30.8675 0.075 4458 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2 39.1759 -13.1386 67.4879 0.075 1602 -1604 2 39.4721 -10.1434 68.4624 0.075 1603 -1605 2 40.1948 -7.34936 69.7466 0.075 1604 -1606 2 40.4214 -4.51909 71.1535 0.075 1605 -1607 2 40.6278 -1.63731 72.267 0.075 1606 -1608 2 41.6145 0.443868 74.3238 0.075 1607 -1609 2 42.8867 2.61877 76.1227 0.075 1608 -1610 2 44.0502 4.99818 77.6139 0.075 1609 -1611 2 44.4978 7.99147 78.2305 0.075 1610 -1612 2 45.2127 10.1829 80.1305 0.075 1611 -1613 2 46.2883 12.1765 82.2303 0.075 1612 -1614 2 47.1733 14.7718 83.5739 0.075 1613 -1615 2 47.0778 17.4714 85.0184 0.075 1614 -1616 2 46.5827 20.4161 85.6588 0.075 1615 -1617 2 47.2627 23.0745 86.9378 0.075 1616 -1618 2 48.1938 25.6673 88.3336 0.075 1617 -1619 2 48.5155 27.9313 90.3268 0.075 1618 -1620 2 48.4676 30.1931 92.2555 0.075 1619 -1621 2 47.7775 32.9388 93.5024 0.075 1620 -1622 2 47.5301 35.8012 94.5991 0.075 1621 -1623 2 47.7205 38.2326 96.4737 0.075 1622 -1624 2 48.1799 40.5457 98.47 0.075 1623 -1625 2 48.346 43.2269 99.8817 0.075 1624 -1626 2 47.4485 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0.075 1831 -1833 2 -56.0901 16.5097 12.9449 0.075 1832 -1834 2 -57.7391 18.8905 11.8885 0.075 1833 -1835 2 -59.8194 21.1724 11.4776 0.075 1834 -1836 2 -61.7148 23.263 10.3129 0.075 1835 -1837 2 -61.8059 25.9987 9.10191 0.075 1836 -1838 2 -61.4286 29.0835 9.08706 0.075 1837 -1839 2 -61.3922 31.3441 7.29109 0.075 1838 -1840 2 -61.2402 33.5186 5.19132 0.075 1839 -1841 2 -61.1504 36.4695 4.23606 0.075 1840 -1842 2 -61.5344 39.3991 3.30009 0.075 1841 -1843 2 -62.232 41.8993 1.60341 0.075 1842 -1844 2 -62.869 44.7616 0.609019 0.075 1843 -1845 2 -63.5188 47.7835 0.208991 0.075 1844 -1846 2 -63.8763 50.8593 -0.151419 0.075 1845 -1847 2 -64.5535 53.8844 -0.487656 0.075 1846 -1848 2 -64.8653 56.812 -1.39665 0.075 1847 -1849 2 -64.8269 59.2679 -3.29674 0.075 1848 -1850 2 -65.7819 61.8685 -4.46178 0.075 1849 -1851 2 -67.0341 62.7563 -0.738271 0.075 1850 -1852 2 -69.499 63.5664 2.20842 0.075 1851 -1853 2 -72.4 64.504 4.72778 0.075 1852 -1854 2 -75.8412 65.8671 6.32039 0.075 1853 -1855 2 -63.8019 66.5685 -6.02178 0.075 1850 -1856 2 -60.8824 68.5371 -3.85763 0.075 1855 -1857 2 -58.351 71.0109 -2.28189 0.075 1856 -1858 2 -56.9631 73.7358 -5.0651 0.075 1857 -1859 2 -64.6454 69.6013 -6.45027 0.075 1855 -1860 2 -64.3723 72.7249 -6.51863 0.075 1859 -1861 2 -64.6218 75.6556 -6.745 0.075 1860 -1862 2 -66.4983 77.9969 -7.5688 0.075 1861 -1863 2 -68.2135 80.4219 -6.6747 0.075 1862 -1864 2 -69.217 82.9103 -4.98827 0.075 1863 -1865 2 -70.7915 85.1187 -3.36224 0.075 1864 -1866 2 -72.3404 87.7238 -2.74927 0.075 1865 -1867 2 -73.2916 90.7356 -3.00106 0.075 1866 -1868 2 -72.3794 95.1958 -2.58832 0.075 1867 -1869 2 -71.1928 98.4232 -5.58773 0.075 1868 -1870 2 -75.4912 93.4865 -3.5164 0.075 1867 -1871 2 -75.9786 96.2958 -5.81597 0.075 1870 -1872 2 -77.0408 99.3224 -7.49319 0.075 1871 -1873 2 -78.885 102.418 -8.00897 0.075 1872 -1874 2 -78.6655 104.303 -11.6317 0.075 1873 -1875 2 -79.0992 108.083 -13.1327 0.075 1874 -1876 2 -79.7154 112.221 -13.1697 0.075 1875 -1877 2 -80.0975 103.436 -5.07443 0.075 1873 -1878 2 -82.4532 104.742 -3.18881 0.075 1877 -1879 2 -85.1884 106.451 -2.3848 0.075 1878 -1880 2 -87.6639 108.403 -1.40936 0.075 1879 -1881 2 -89.6656 111.058 -1.32336 0.075 1880 -1882 2 -91.7791 113.575 -0.754245 0.075 1881 -1883 2 -93.6474 116.072 0.427838 0.075 1882 -1884 2 -95.705 118.47 1.3805 0.075 1883 -1885 2 -98.4192 120.28 0.76491 0.075 1884 -1886 2 -101.101 122.227 0.824453 0.075 1885 -1887 2 -103.474 124.25 2.00654 0.075 1886 -1888 2 -105.391 126.832 2.81698 0.075 1887 -1889 2 -51.5542 15.6069 14.7584 0.075 1831 -1890 2 -50.4876 18.6046 14.319 0.075 1889 -1891 2 -49.1425 21.4607 13.7046 0.075 1890 -1892 2 -48.0506 24.4221 13.1073 0.075 1891 -1893 2 -47.067 27.4637 12.7733 0.075 1892 -1894 2 -46.5217 30.6114 12.7339 0.075 1893 -1895 2 -46.3284 33.7073 11.9763 0.075 1894 -1896 2 -46.5901 36.7396 11.1553 0.075 1895 -1897 2 -47.1124 39.7359 12.0116 0.075 1896 -1898 2 -40.4925 -6.73833 25.3695 0.075 1823 -1899 2 -41.4655 -5.47036 28.3323 0.075 1898 -1900 2 -42.6534 -4.02915 31.1242 0.075 1899 -1901 2 -44.1449 -3.07272 33.8911 0.075 1900 -1902 2 -46.9777 -1.81356 34.7477 0.075 1901 -1903 2 -49.8286 -1.15646 36.3262 0.075 1902 -1904 2 -52.5406 -0.806188 38.2791 0.075 1903 -1905 2 -55.6698 -0.680077 39.4914 0.075 1904 -1906 2 -58.877 -0.423508 40.3977 0.075 1905 -1907 2 -60.6128 -0.240443 43.2767 0.075 1906 -1908 2 -62.4847 0.050177 46.0565 0.075 1907 -1909 2 -10.9143 -49.7058 30.0724 0.075 1805 -1910 2 -12.5177 -52.0984 28.7281 0.075 1909 -1911 2 -14.9001 -54.1185 28.791 0.075 1910 -1912 2 -17.6541 -55.6988 29.3144 0.075 1911 -1913 2 -20.4148 -57.1695 30.0121 0.075 1912 -1914 2 -23.1576 -58.739 30.5467 0.075 1913 -1915 2 -26.1469 -59.9073 30.8055 0.075 1914 -1916 2 -28.8176 -61.641 31.1042 0.075 1915 -1917 2 -27.2575 -62.9447 33.7629 0.075 1916 -1918 2 -24.7511 -65.207 33.8509 0.075 1917 -1919 2 -23.2992 -68.1905 33.8394 0.075 1918 -1920 2 -22.7549 -71.106 34.8159 0.075 1919 -1921 2 -21.8147 -72.5335 37.7155 0.075 1920 -1922 2 -32.6226 -63.1082 30.8929 0.075 1916 -1923 2 -36.2492 -64.3782 29.5879 0.075 1922 -1924 2 -36.2138 -66.8971 25.9742 0.075 1923 -1925 2 -35.6277 -69.1662 22.2552 0.075 1924 -1926 2 -37.7592 -64.9582 29.6813 0.075 1923 -1927 2 -38.9571 -67.9434 30.1564 0.075 1926 -1928 2 -41.0875 -70.3414 30.6336 0.075 1927 -1929 2 -43.7323 -72.1692 31.0268 0.075 1928 -1930 2 -46.4595 -73.8985 31.3382 0.075 1929 -1931 2 -48.5725 -76.322 31.5093 0.075 1930 -1932 2 -50.7512 -78.6747 31.4347 0.075 1931 -1933 2 -53.7202 -79.9063 30.9385 0.075 1932 -1934 2 -56.6577 -81.2678 30.6754 0.075 1933 -1935 2 -59.5967 -82.454 29.9778 0.075 1934 -1936 2 -59.9803 -83.3683 32.0466 0.075 1935 -1937 2 -58.8961 -84.5002 34.6168 0.075 1936 -1938 2 -56.7435 -86.0304 36.4949 0.075 1937 -1939 2 -40.2473 -63.1297 29.7594 0.075 1926 -1940 2 -43.0523 -62.8996 30.8411 0.075 1939 -1941 2 -45.8933 -63.7706 31.56 0.075 1940 -1942 2 -48.9619 -63.9588 31.7839 0.075 1941 -1943 2 -52.0457 -63.8 31.9656 0.075 1942 -1944 2 -55.0323 -63.3682 32.495 0.075 1943 -1945 2 -57.4641 -62.6522 34.182 0.075 1944 -1946 2 -59.5866 -62.5596 36.3744 0.075 1945 -1947 2 -62.4057 -62.6848 37.6079 0.075 1946 -1948 2 -65.396 -62.5798 38.3782 0.075 1947 -1949 2 -68.3119 -62.369 39.3047 0.075 1948 -1950 2 -71.0148 -61.7091 40.6469 0.075 1949 -1951 2 -73.7973 -61.2205 41.8935 0.075 1950 -1952 2 -76.6196 -61.0148 43.1426 0.075 1951 -1953 2 -79.5276 -60.8218 44.17 0.075 1952 -1954 2 -82.258 -60.4599 45.4705 0.075 1953 -1955 2 -85.0278 -60.1192 46.7314 0.075 1954 -1956 2 -88.0591 -59.6373 47.0822 0.075 1955 -1957 2 -91.0095 -59.2214 47.8133 0.075 1956 -1958 2 -93.1004 -58.7685 49.9617 0.075 1957 -1959 2 -94.3265 -58.7495 52.7824 0.075 1958 -1960 2 25.4789 -69.2039 17.7147 0.0791639 905 -1961 2 27.2784 -68.1375 15.0552 0.075 1960 -1962 2 28.6841 -65.2881 13.7346 0.075 1961 -1963 2 29.6656 -61.8834 13.6229 0.075 1962 -1964 2 31.8472 -64.5972 11.8161 0.075 1963 -1965 2 33.569 -68.1821 12.1529 0.075 1964 -1966 2 35.4345 -71.6955 12.4747 0.075 1965 -1967 2 33.3074 -73.722 9.3152 0.075 1966 -1968 2 31.3369 -75.9438 6.25686 0.075 1967 -1969 2 28.3299 -78.8032 5.16459 0.075 1968 -1970 2 26.5602 -82.061 4.70526 0.075 1969 -1971 2 25.6894 -85.0797 2.54244 0.075 1970 -1972 2 25.0839 -88.8042 2.06384 0.075 1971 -1973 2 24.3764 -92.4977 1.40189 0.075 1972 -1974 2 24.0242 -95.624 0.770155 0.075 1973 -1975 2 23.4297 -97.9078 -1.35996 0.075 1974 -1976 2 23.0516 -99.8285 -3.90639 0.075 1975 -1977 2 22.0474 -101.069 -6.62958 0.075 1976 -1978 2 20.22 -103.125 -8.0877 0.075 1977 -1979 2 19.2758 -104.702 -10.4494 0.075 1978 -1980 2 19.7993 -106.278 -13.1122 0.075 1979 -1981 2 20.4379 -109.153 -13.6283 0.075 1980 -1982 2 21.3793 -92.9692 0.5208 0.075 1973 -1983 2 19.7301 -94.8536 -1.46972 0.075 1982 -1984 2 18.3295 -97.4944 -2.60233 0.075 1983 -1985 2 16.5364 -100.174 -2.69635 0.075 1984 -1986 2 14.1617 -102.313 -3.11635 0.075 1985 -1987 2 11.3487 -103.88 -2.94394 0.075 1986 -1988 2 8.4388 -105.283 -2.71595 0.075 1987 -1989 2 5.7764 -106.776 -3.58054 0.075 1988 -1990 2 3.22185 -107.703 -5.32943 0.075 1989 -1991 2 0.576516 -108.356 -7.08114 0.075 1990 -1992 2 -1.69127 -110.241 -8.25919 0.075 1991 -1993 2 -4.06448 -112.43 -8.2739 0.075 1992 -1994 2 -6.42368 -114.494 -8.92141 0.075 1993 -1995 2 -8.81152 -116.523 -9.46155 0.075 1994 -1996 2 26.9716 -77.1206 2.45065 0.075 1969 -1997 2 24.9761 -76.0018 -0.0759544 0.075 1996 -1998 2 22.4369 -74.6072 -1.98558 0.075 1997 -1999 2 20.7426 -73.4153 -4.75347 0.075 1998 -2000 2 18.9142 -72.4259 -7.51545 0.075 1999 -2001 2 16.8096 -71.5827 -9.97373 0.075 2000 -2002 2 16.9103 -71.0636 -12.1319 0.075 2001 -2003 2 17.4278 -74.459 -10.7307 0.075 2002 -2004 2 18.468 -77.8971 -10.3655 0.075 2003 -2005 2 20.899 -80.6697 -10.1789 0.075 2004 -2006 2 22.3313 -84.082 -9.8443 0.075 2005 -2007 2 19.1713 -86.8679 -9.48681 0.075 2006 -2008 2 16.578 -89.0355 -12.1507 0.075 2007 -2009 2 17.1069 -92.5839 -11.3914 0.075 2008 -2010 2 24.6502 -86.167 -9.86727 0.075 2006 -2011 2 26.1976 -88.8786 -10.0248 0.075 2010 -2012 2 27.3061 -91.7748 -10.1159 0.075 2011 -2013 2 27.6013 -94.8408 -10.6327 0.075 2012 -2014 2 27.9619 -97.9146 -10.9548 0.075 2013 -2015 2 28.4774 -100.99 -10.7618 0.075 2014 -2016 2 29.0253 -104.056 -10.5015 0.075 2015 -2017 2 29.4116 -107.156 -10.3807 0.075 2016 -2018 2 30.1551 -110.162 -10.2209 0.075 2017 -2019 2 30.8802 -113.081 -9.47205 0.075 2018 -2020 2 32.2597 -115.725 -8.63033 0.075 2019 -2021 2 34.0773 -118.239 -8.76291 0.075 2020 -2022 2 35.8249 -120.772 -8.49867 0.075 2021 -2023 2 37.6364 -123.16 -7.61536 0.075 2022 -2024 2 39.0804 -125.886 -7.14888 0.075 2023 -2025 2 39.8674 -128.851 -6.65692 0.075 2024 -2026 2 39.9252 -131.355 -8.49387 0.075 2025 -2027 2 40.4371 -134.081 -9.82693 0.075 2026 -2028 2 41.4433 -137.007 -10.1761 0.075 2027 -2029 2 42.0934 -139.758 -11.3528 0.075 2028 -2030 2 42.304 -142.703 -12.1719 0.075 2029 -2031 2 42.791 -145.774 -11.8802 0.075 2030 -2032 2 43.6796 -148.759 -11.6747 0.075 2031 -2033 2 44.5964 -151.697 -12.012 0.075 2032 -2034 2 45.6313 -154.578 -12.5387 0.075 2033 -2035 2 47.1591 -157.296 -12.3793 0.075 2034 -2036 2 48.7805 -159.956 -12.4067 0.075 2035 -2037 2 16.5064 -67.7279 -12.6591 0.075 2002 -2038 2 15.8113 -64.6014 -13.7021 0.075 2037 -2039 2 16.4875 -61.3918 -13.7182 0.075 2038 -2040 2 17.3073 -58.2018 -13.1639 0.075 2039 -2041 2 18.3527 -55.0903 -14.0299 0.075 2040 -2042 2 19.7291 -52.1467 -15.0649 0.075 2041 -2043 2 23.2419 -52.7908 -19.4433 0.075 2042 -2044 2 17.5088 -49.2162 -14.2359 0.075 2042 -2045 2 15.9813 -46.3818 -12.4262 0.075 2044 -2046 2 16.3027 -43.494 -10.021 0.075 2045 -2047 2 15.6406 -40.7828 -7.5426 0.075 2046 -2048 2 14.7719 -38.2994 -4.84965 0.075 2047 -2049 2 17.2091 -39.8628 -6.76112 0.075 2048 -2050 2 19.4799 -42.0808 -4.91074 0.075 2049 -2051 2 21.7571 -43.0973 -2.28648 0.075 2050 -2052 2 22.6355 -44.7227 -4.79919 0.075 2051 -2053 2 24.4674 -46.5302 -6.15443 0.075 2052 -2054 2 26.7679 -48.1746 -5.10589 0.075 2053 -2055 2 28.5406 -50.2498 -3.65309 0.075 2054 -2056 2 30.4504 -52.6467 -3.20246 0.075 2055 -2057 2 32.5703 -54.9187 -3.00439 0.075 2056 -2058 2 35.0383 -56.8058 -2.77024 0.075 2057 -2059 2 37.7294 -58.3458 -2.55756 0.075 2058 -2060 2 40.1544 -60.282 -2.29404 0.075 2059 -2061 2 42.6386 -61.8621 -1.31051 0.075 2060 -2062 2 44.9732 -63.5764 -0.195943 0.075 2061 -2063 2 46.8746 -65.899 0.595862 0.075 2062 -2064 2 49.0417 -68.1188 0.843792 0.075 2063 -2065 2 51.6604 -69.702 0.476968 0.075 2064 -2066 2 54.8942 -68.0408 -1.43992 0.075 2065 -2067 2 57.8617 -66.5052 0.267736 0.075 2066 -2068 2 61.3833 -64.8099 -0.169853 0.075 2067 -2069 2 65.0722 -63.4801 -0.505024 0.075 2068 -2070 2 68.5183 -61.634 -0.834165 0.075 2069 -2071 2 71.6415 -59.3715 -1.57753 0.075 2070 -2072 2 60.7968 -67.6835 0.204246 0.075 2067 -2073 2 63.8837 -68.2896 0.0966737 0.075 2072 -2074 2 66.8609 -69.0645 0.521223 0.075 2073 -2075 2 69.7134 -70.376 0.816225 0.075 2074 -2076 2 72.546 -71.7712 0.901825 0.075 2075 -2077 2 75.4067 -72.9048 1.56273 0.075 2076 -2078 2 77.7735 -73.7429 3.31281 0.075 2077 -2079 2 79.5366 -74.8318 5.66971 0.075 2078 -2080 2 82.0496 -76.2346 6.75997 0.075 2079 -2081 2 84.7931 -77.763 6.55939 0.075 2080 -2082 2 87.3886 -79.2101 5.74402 0.075 2081 -2083 2 90.1576 -79.8315 4.35876 0.075 2082 -2084 2 93.1729 -80.3241 3.58089 0.075 2083 -2085 2 96.3126 -80.5034 3.27346 0.075 2084 -2086 2 99.7686 -81.8547 3.22325 0.075 2085 -2087 2 103.226 -82.8523 2.56724 0.075 2086 -2088 2 106.542 -83.9061 1.2835 0.075 2087 -2089 2 109.634 -85.3892 0.0343966 0.075 2088 -2090 2 112.151 -87.3312 -1.76595 0.075 2089 -2091 2 114.575 -87.7556 -4.49413 0.075 2090 -2092 2 101.711 -77.9889 4.6438 0.075 2085 -2093 2 52.5972 -72.3317 -1.59517 0.075 2065 -2094 2 54.1884 -74.3523 -3.91633 0.075 2093 -2095 2 55.7256 -76.4618 -6.18532 0.075 2094 -2096 2 57.2431 -78.7218 -8.33254 0.075 2095 -2097 2 58.0884 -79.4228 -5.1555 0.075 2096 -2098 2 58.129 -80.407 -1.94936 0.075 2097 -2099 2 58.6748 -81.2086 1.25839 0.075 2098 -2100 2 59.0139 -83.1634 3.84538 0.075 2099 -2101 2 59.2372 -85.5716 5.99274 0.075 2100 -2102 2 60.4019 -86.4211 8.88774 0.075 2101 -2103 2 59.5427 -80.7546 -10.4946 0.075 2096 -2104 2 61.694 -82.8324 -12.5298 0.075 2103 -2105 2 63.7955 -85.6524 -13.8098 0.075 2104 -2106 2 59.0155 -85.7824 -16.4898 0.075 2105 -2107 2 66.3169 -87.5511 -14.03 0.075 2105 -2108 2 67.9259 -90.0695 -14.6544 0.075 2107 -2109 2 68.6157 -93.1362 -14.9818 0.075 2108 -2110 2 70.4399 -95.6672 -15.2971 0.075 2109 -2111 2 72.5498 -97.704 -16.4205 0.075 2110 -2112 2 74.2689 -100.202 -17.21 0.075 2111 -2113 2 75.974 -102.83 -17.5561 0.075 2112 -2114 2 78.0918 -105.069 -18.1685 0.075 2113 -2115 2 80.4431 -106.997 -17.5236 0.075 2114 -2116 2 82.6866 -108.981 -16.5224 0.075 2115 -2117 2 84.7088 -111.358 -16.0812 0.075 2116 -2118 2 86.8226 -113.615 -16.5832 0.075 2117 -2119 2 88.9148 -115.768 -17.5288 0.075 2118 -2120 2 91.5652 -117.388 -17.6083 0.075 2119 -2121 2 94.3662 -118.804 -17.5804 0.075 2120 -2122 2 96.5742 -120.732 -18.6419 0.075 2121 -2123 2 98.2926 -123.312 -18.9655 0.075 2122 -2124 2 99.5393 -126.207 -18.688 0.075 2123 -2125 2 24.3227 -41.2839 -2.55836 0.075 2051 -2126 2 27.0385 -40.2622 -3.22694 0.075 2125 -2127 2 30.1051 -40.7336 -2.90794 0.075 2126 -2128 2 32.3288 -41.6291 -4.68839 0.075 2127 -2129 2 34.0949 -42.4133 -7.19985 0.075 2128 -2130 2 35.9654 -43.1135 -9.63216 0.075 2129 -2131 2 37.1521 -42.9781 -12.5423 0.075 2130 -2132 2 37.7557 -42.1299 -15.5218 0.075 2131 -2133 2 38.9423 -40.6347 -17.9768 0.075 2132 -2134 2 41.6007 -39.4255 -19.2033 0.075 2133 -2135 2 44.3516 -38.761 -20.596 0.075 2134 -2136 2 13.4266 -35.8946 -2.06541 0.075 2048 -2137 2 11.6274 -33.825 0.638335 0.075 2136 -2138 2 10.4968 -30.1455 1.74033 0.075 2137 -2139 2 12.563 -29.3941 -0.751675 0.075 2138 -2140 2 14.9835 -27.92 -2.46419 0.075 2139 -2141 2 17.2599 -26.256 -4.21712 0.075 2140 -2142 2 20.1824 -25.3469 -5.43152 0.075 2141 -2143 2 23.33 -24.9525 -5.60283 0.075 2142 -2144 2 26.2021 -24.9641 -4.00746 0.075 2143 -2145 2 29.3237 -25.3829 -3.518 0.075 2144 -2146 2 31.9083 -25.808 -5.38294 0.075 2145 -2147 2 9.21951 -29.2036 5.03121 0.075 2138 -2148 2 6.84284 -27.1705 6.90526 0.075 2147 -2149 2 5.2135 -24.2571 8.23481 0.075 2148 -2150 2 4.32921 -21.8214 10.8204 0.075 2149 -2151 2 3.28007 -19.1269 13.0228 0.075 2150 -2152 2 37.269 -74.8422 12.357 0.075 1966 -2153 2 39.2254 -77.9081 12.5951 0.075 2152 -2154 2 41.0694 -81.0382 12.8665 0.075 2153 -2155 2 39.4181 -82.9679 10.873 0.075 2154 -2156 2 38.3052 -85.9955 10.743 0.075 2155 -2157 2 37.6943 -89.1679 10.8364 0.075 2156 -2158 2 36.9344 -91.923 9.40509 0.075 2157 -2159 2 36.2363 -94.3974 7.42678 0.075 2158 -2160 2 35.551 -97.4062 6.56262 0.075 2159 -2161 2 34.6773 -100.44 5.91756 0.075 2160 -2162 2 33.1883 -103.294 5.56211 0.075 2161 -2163 2 31.9564 -106.268 5.24446 0.075 2162 -2164 2 45.7894 -85.8182 12.9865 0.075 2154 -2165 2 42.2994 -84.0082 17.4265 0.075 2164 -2166 2 48.2476 -88.4044 13.6353 0.075 2164 -2167 2 50.2128 -90.0924 16.1551 0.075 2166 -2168 2 52.6631 -91.1667 18.5552 0.075 2167 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-104.425 26.5245 0.295 1791 -1793 2 32.6983 -107.417 26.8525 0.293206 1792 -1794 2 33.5254 -110.409 27.3351 0.288202 1793 -1795 2 34.5437 -113.353 27.7376 0.270436 1794 -1796 2 35.7163 -116.243 28.0753 0.26 1795 -1797 2 37.0508 -119.074 28.3451 0.26 1796 -1798 2 36.3708 -121.994 28.7584 0.26 1797 -1799 2 38.8832 -125.19 30.4364 0.145 1798 -1800 2 41.4234 -128.829 30.8277 0.145 1799 -1801 2 41.667 -132.306 31.4041 0.11 1800 -1802 2 42.7118 -135.672 31.7291 0.11 1801 -1803 2 43.5352 -139.103 31.9393 0.11 1802 -1804 2 44.306 -142.553 31.9946 0.11 1803 -1805 2 45.155 -145.985 31.8544 0.11 1804 -1806 2 46.1273 -149.464 32.0586 0.103801 1805 -1807 2 47.5516 -152.779 32.3341 0.0817646 1806 -1808 2 49.4473 -155.833 32.5473 0.075 1807 -1809 2 51.6296 -158.711 32.737 0.075 1808 -1810 2 53.9587 -161.474 32.9343 0.075 1809 -1811 2 52.4487 -167.274 32.7477 0.075 1810 -1812 2 54.2772 -169.765 31.9393 0.075 1811 -1813 2 55.7225 -172.594 32.2858 0.075 1812 -1814 2 57.5903 -175.102 32.7639 0.075 1813 -1815 2 59.6515 -177.462 33.3031 0.075 1814 -1816 2 61.1297 -180.273 33.6649 0.075 1815 -1817 2 62.2376 -183.255 33.7298 0.075 1816 -1818 2 63.1558 -186.305 33.5674 0.075 1817 -1819 2 63.9429 -189.37 33.1228 0.075 1818 -1820 2 64.9364 -192.361 32.5981 0.075 1819 -1821 2 65.9603 -195.361 32.3144 0.075 1820 -1822 2 66.982 -198.389 32.3628 0.075 1821 -1823 2 64.1255 -199.921 29.5017 0.075 1822 -1824 2 60.3665 -200.137 27.2467 0.075 1823 -1825 2 56.8773 -199.52 24.6411 0.075 1824 -1826 2 55.2503 -201.537 22.7569 0.075 1825 -1827 2 52.0674 -202.165 22.3102 0.075 1826 -1828 2 49.0505 -201.488 21.744 0.075 1827 -1829 2 46.2492 -200.187 20.8332 0.075 1828 -1830 2 43.9273 -198.912 18.9074 0.075 1829 -1831 2 42.0331 -196.949 17.156 0.075 1830 -1832 2 40.2856 -194.795 15.4167 0.075 1831 -1833 2 38.5668 -192.692 13.5864 0.075 1832 -1834 2 37.3005 -190.718 11.3164 0.075 1833 -1835 2 35.6629 -188.83 9.32369 0.075 1834 -1836 2 33.335 -187.04 7.87348 0.075 1835 -1837 2 31.865 -188.04 9.76681 0.075 1836 -1838 2 31.6934 -191.415 12.1105 0.075 1837 -1839 2 30.9293 -195.319 13.1204 0.075 1838 -1840 2 29.5928 -199.075 13.9077 0.075 1839 -1841 2 27.6517 -202.653 14.4956 0.075 1840 -1842 2 25.0576 -206.184 14.5396 0.075 1841 -1843 2 24.0309 -210.21 16.1861 0.075 1842 -1844 2 23.3249 -212.979 17.3338 0.075 1843 -1845 2 23.1411 -216.028 17.7307 0.075 1844 -1846 2 23.1764 -219.045 18.3055 0.075 1845 -1847 2 23.3028 -222.033 19.0288 0.075 1846 -1848 2 23.1239 -224.992 19.8646 0.075 1847 -1849 2 22.6388 -227.922 20.662 0.075 1848 -1850 2 22.1258 -230.84 21.4971 0.075 1849 -1851 2 21.7561 -233.752 22.4313 0.075 1850 -1852 2 21.6223 -236.724 23.2221 0.075 1851 -1853 2 21.6556 -239.687 24.05 0.075 1852 -1854 2 21.7799 -242.628 24.9396 0.075 1853 -1855 2 21.3325 -245.5 25.9611 0.075 1854 -1856 2 20.3639 -248.292 25.8417 0.075 1855 -1857 2 19.1141 -250.862 24.8342 0.075 1856 -1858 2 17.7923 -253.077 23.1502 0.075 1857 -1859 2 15.7343 -255.229 22.4272 0.075 1858 -1860 2 13.3055 -257.005 22.3091 0.075 1859 -1861 2 10.6589 -258.442 22.7924 0.075 1860 -1862 2 8.20366 -259.622 24.2304 0.075 1861 -1863 2 6.50796 -261.074 26.2828 0.075 1862 -1864 2 5.09965 -262.892 28.2705 0.075 1863 -1865 2 3.81616 -265.206 29.8473 0.075 1864 -1866 2 2.45283 -267.644 31.1442 0.075 1865 -1867 2 0.891686 -269.972 32.4047 0.075 1866 -1868 2 -0.811894 -272.255 33.5239 0.075 1867 -1869 2 -2.36499 -274.9 33.8079 0.075 1868 -1870 2 -3.80159 -277.176 32.5984 0.075 1869 -1871 2 -5.07402 -279.526 31.1394 0.075 1870 -1872 2 -6.09523 -282.261 30.1574 0.075 1871 -1873 2 -5.979 -285.975 31.2682 0.075 1872 -1874 2 -6.57369 -289.798 31.4615 0.075 1873 -1875 2 -7.5793 -293.505 31.3284 0.075 1874 -1876 2 -8.99899 -297.086 30.8769 0.075 1875 -1877 2 -7.07204 -284.688 32.0045 0.075 1872 -1878 2 -7.84431 -287.118 33.9421 0.075 1877 -1879 2 -8.63925 -289.637 35.7481 0.075 1878 -1880 2 -9.51209 -292.241 37.3818 0.075 1879 -1881 2 -10.6107 -294.94 38.7091 0.075 1880 -1882 2 -11.7341 -296.867 40.9519 0.075 1881 -1883 2 -12.8278 -298.259 43.5784 0.075 1882 -1884 2 -13.9014 -299.172 46.4478 0.075 1883 -1885 2 -15.1269 -299.523 49.3856 0.075 1884 -1886 2 -16.2761 -300.158 52.2998 0.075 1885 -1887 2 -17.4778 -301.04 55.1222 0.075 1886 -1888 2 -18.8799 -302.209 57.7535 0.075 1887 -1889 2 -20.3713 -303.981 59.9513 0.075 1888 -1890 2 -21.6127 -306.331 61.5806 0.075 1889 -1891 2 -22.4421 -309.16 62.7476 0.075 1890 -1892 2 -22.1601 -312.119 63.9398 0.075 1891 -1893 2 -20.6929 -314.614 65.111 0.075 1892 -1894 2 -18.5101 -316.666 66.0413 0.075 1893 -1895 2 -15.7847 -318.251 66.6031 0.075 1894 -1896 2 26.0997 -212.038 14.7082 0.075 1843 -1897 2 27.8707 -213.733 12.7549 0.075 1896 -1898 2 29.2991 -215.432 10.56 0.075 1897 -1899 2 30.7714 -217.135 8.41961 0.075 1898 -1900 2 32.7459 -218.849 6.6904 0.075 1899 -1901 2 35.6343 -219.959 6.56178 0.075 1900 -1902 2 38.3961 -221.341 6.88831 0.075 1901 -1903 2 40.9771 -223.027 7.41073 0.075 1902 -1904 2 43.3709 -225.028 7.7148 0.075 1903 -1905 2 45.9024 -226.678 7.02655 0.075 1904 -1906 2 48.6047 -227.935 6.1339 0.075 1905 -1907 2 51.4947 -228.755 5.27228 0.075 1906 -1908 2 54.5954 -228.964 4.86215 0.075 1907 -1909 2 57.6496 -229.529 4.51148 0.075 1908 -1910 2 60.5675 -230.536 4.21832 0.075 1909 -1911 2 63.2387 -232.161 3.99418 0.075 1910 -1912 2 65.1651 -234.504 3.25141 0.075 1911 -1913 2 66.797 -236.966 2.24616 0.075 1912 -1914 2 68.2294 -239.416 0.946977 0.075 1913 -1915 2 69.6036 -241.583 -0.853323 0.075 1914 -1916 2 70.525 -243.467 -3.15812 0.075 1915 -1917 2 70.9973 -245.337 -5.59845 0.075 1916 -1918 2 71.1346 -247.232 -8.07031 0.075 1917 -1919 2 72.355 -248.708 -10.5522 0.075 1918 -1920 2 73.9717 -250.397 -12.6073 0.075 1919 -1921 2 75.5142 -252.225 -14.6082 0.075 1920 -1922 2 76.6831 -254.165 -16.7754 0.075 1921 -1923 2 24.4013 -201.389 14.9283 0.075 1841 -1924 2 20.9563 -201.3 15.7467 0.075 1923 -1925 2 17.5369 -201.191 16.6613 0.075 1924 -1926 2 14.1142 -201.263 17.4815 0.075 1925 -1927 2 10.6504 -201.857 17.9234 0.075 1926 -1928 2 7.27841 -202.577 18.7166 0.075 1927 -1929 2 3.95007 -203.419 19.5787 0.075 1928 -1930 2 0.658814 -204.401 20.4442 0.075 1929 -1931 2 31.6587 -186.712 6.90508 0.075 1837 -1932 2 30.1233 -185.698 4.3407 0.075 1931 -1933 2 27.928 -185.068 2.24389 0.075 1932 -1934 2 25.5272 -184.737 0.244013 0.075 1933 -1935 2 23.4493 -184.716 -2.13882 0.075 1934 -1936 2 22.1086 -184.795 -4.97853 0.075 1935 -1937 2 20.8914 -184.891 -7.8949 0.075 1936 -1938 2 19.6747 -184.984 -10.8114 0.075 1937 -1939 2 18.5154 -184.764 -13.7445 0.075 1938 -1940 2 17.5597 -184.55 -16.7454 0.075 1939 -1941 2 16.7693 -184.387 -19.8 0.075 1940 -1942 2 16.0979 -184.444 -22.8889 0.075 1941 -1943 2 15.4266 -184.501 -25.9779 0.075 1942 -1944 2 14.4575 -183.949 -28.7385 0.075 1943 -1945 2 12.895 -182.183 -30.8447 0.075 1944 -1946 2 30.465 -182.65 6.51348 0.075 1836 -1947 2 28.6931 -184.144 4.31706 0.075 1946 -1948 2 26.4339 -185.541 2.54564 0.075 1947 -1949 2 24.0489 -187.025 1.0503 0.075 1948 -1950 2 21.6477 -188.61 0.4721 0.075 1949 -1951 2 19.4588 -190.308 2.06009 0.075 1950 -1952 2 17.2841 -192.534 2.53693 0.075 1951 -1953 2 15.5539 -194.562 1.26263 0.075 1952 -1954 2 14.0769 -196.489 -0.805535 0.075 1953 -1955 2 12.6335 -198.62 -2.69487 0.075 1954 -1956 2 11.7021 -201.151 -4.37917 0.075 1955 -1957 2 10.9351 -203.795 -5.99381 0.075 1956 -1958 2 10.2637 -206.502 -7.52426 0.075 1957 -1959 2 9.83871 -209.666 -7.47086 0.075 1958 -1960 2 9.18407 -212.68 -7.84112 0.075 1959 -1961 2 8.33432 -214.735 -10.1326 0.075 1960 -1962 2 7.39893 -217.313 -11.6415 0.075 1961 -1963 2 6.33166 -220.182 -12.3128 0.075 1962 -1964 2 5.12493 -223.138 -12.2575 0.075 1963 -1965 2 28.8404 -179.883 5.54592 0.075 1946 -1966 2 26.7079 -177.355 5.03345 0.075 1965 -1967 2 24.6237 -174.761 4.67679 0.075 1966 -1968 2 22.6348 -172.071 4.47291 0.075 1967 -1969 2 20.9589 -169.18 4.22189 0.075 1968 -1970 2 19.0592 -166.444 4.00394 0.075 1969 -1971 2 16.9006 -163.886 3.82431 0.075 1970 -1972 2 14.9374 -164.319 0.592044 0.075 1971 -1973 2 12.2658 -165.948 -1.59861 0.075 1972 -1974 2 9.81023 -168.498 -2.10295 0.075 1973 -1975 2 7.39328 -171.427 -1.71994 0.075 1974 -1976 2 4.85497 -174.234 -1.20206 0.075 1975 -1977 2 3.50097 -172.182 0.686667 0.075 1976 -1978 2 2.21989 -169.459 1.43507 0.075 1977 -1979 2 0.629961 -166.855 1.79394 0.075 1978 -1980 2 -0.941667 -164.595 2.51619 0.075 1979 -1981 2 -1.8416 -163.114 5.08745 0.075 1980 -1982 2 -2.24611 -161.507 7.69882 0.075 1981 -1983 2 -2.44778 -159.551 10.0403 0.075 1982 -1984 2 -2.49901 -157.148 11.9985 0.075 1983 -1985 2 -2.67245 -154.449 13.5154 0.075 1984 -1986 2 -3.13384 -151.842 15.1079 0.075 1985 -1987 2 -3.89213 -149.395 16.8256 0.075 1986 -1988 2 -5.07601 -147.403 18.8867 0.075 1987 -1989 2 -6.59811 -145.347 20.6174 0.075 1988 -1990 2 -8.29478 -143.261 22.1554 0.075 1989 -1991 2 -10.1091 -141.159 23.5354 0.075 1990 -1992 2 -11.2199 -139.451 25.8452 0.075 1991 -1993 2 -12.0614 -138.441 28.5605 0.075 1992 -1994 2 -12.7939 -138.001 31.5348 0.075 1993 -1995 2 -13.9921 -137.503 34.3511 0.075 1994 -1996 2 -15.1904 -137.005 37.1676 0.075 1995 -1997 2 -16.8932 -136.56 39.4308 0.075 1996 -1998 2 -19.8462 -136.245 40.3229 0.075 1997 -1999 2 4.35046 -175.988 -3.96746 0.075 1976 -2000 2 3.59674 -176.227 -7.06467 0.075 1999 -2001 2 2.70453 -175.906 -10.2179 0.075 2000 -2002 2 1.23513 -174.711 -12.9368 0.075 2001 -2003 2 -0.412838 -174.519 -15.5294 0.075 2002 -2004 2 -2.31564 -175.76 -17.9417 0.075 2003 -2005 2 16.2206 -157.136 2.94431 0.075 1971 -2006 2 18.4902 -156.293 0.258536 0.075 2005 -2007 2 20.1792 -155.997 -2.92742 0.075 2006 -2008 2 22.8643 -155.166 -3.45734 0.075 2007 -2009 2 26.2082 -154.668 -2.54983 0.075 2008 -2010 2 29.6884 -155.399 -1.8834 0.075 2009 -2011 2 32.1353 -157.786 -1.59709 0.075 2010 -2012 2 35.0132 -159.322 -0.476476 0.075 2011 -2013 2 37.924 -160.729 0.723264 0.075 2012 -2014 2 41.0002 -162.011 1.32621 0.075 2013 -2015 2 44.2678 -162.965 1.39169 0.075 2014 -2016 2 47.6765 -162.83 1.90185 0.075 2015 -2017 2 50.5114 -163.436 3.6788 0.075 2016 -2018 2 53.0377 -164.308 5.84157 0.075 2017 -2019 2 55.3973 -165.293 8.15587 0.075 2018 -2020 2 57.6919 -166.306 10.5237 0.075 2019 -2021 2 30.8183 -154.218 -4.60493 0.075 2010 -2022 2 32.9747 -153.597 -6.85405 0.075 2021 -2023 2 34.7606 -152.571 -9.23407 0.075 2022 -2024 2 36.5276 -151.351 -11.5388 0.075 2023 -2025 2 38.8248 -150.247 -13.4362 0.075 2024 -2026 2 41.5444 -149.078 -14.5504 0.075 2025 -2027 2 44.1594 -147.975 -15.9286 0.075 2026 -2028 2 46.654 -147.082 -17.6478 0.075 2027 -2029 2 49.2528 -146.986 -19.473 0.075 2028 -2030 2 51.4053 -147.388 -21.7346 0.075 2029 -2031 2 53.6819 -147.576 -23.8513 0.075 2030 -2032 2 56.3441 -147.231 -25.5245 0.075 2031 -2033 2 59.4845 -146.961 -25.1256 0.075 2032 -2034 2 62.5562 -146.672 -24.4037 0.075 2033 -2035 2 65.1961 -146.576 -24.6446 0.075 2034 -2036 2 67.0036 -146.908 -27.2363 0.075 2035 -2037 2 14.9206 -155.206 2.69097 0.075 2005 -2038 2 14.2102 -155.572 6.1695 0.075 2037 -2039 2 12.6644 -156.129 9.34266 0.075 2038 -2040 2 11.2483 -157.001 12.4973 0.075 2039 -2041 2 9.91222 -158.022 15.6501 0.075 2040 -2042 2 8.61723 -159.057 18.8156 0.075 2041 -2043 2 13.7125 -152.023 2.34607 0.075 2037 -2044 2 12.4371 -148.856 2.10526 0.075 2043 -2045 2 11.3045 -145.921 2.87486 0.075 2044 -2046 2 10.3386 -143.246 4.77904 0.075 2045 -2047 2 8.82958 -143.381 2.10643 0.075 2046 -2048 2 7.32061 -143.516 -0.566157 0.075 2047 -2049 2 5.56926 -144.005 -3.00394 0.075 2048 -2050 2 3.60541 -144.878 -5.17524 0.075 2049 -2051 2 1.52172 -146.361 -6.8774 0.075 2050 -2052 2 -1.01709 -147.676 -7.8851 0.075 2051 -2053 2 -3.59684 -149.176 -8.4541 0.075 2052 -2054 2 -5.95854 -151.131 -8.65355 0.075 2053 -2055 2 -7.37625 -153.162 -10.4471 0.075 2054 -2056 2 -8.68537 -155.218 -12.3154 0.075 2055 -2057 2 -9.99465 -157.32 -14.1009 0.075 2056 -2058 2 -11.7481 -159.604 -15.1725 0.075 2057 -2059 2 -13.9334 -161.599 -15.9022 0.075 2058 -2060 2 -16.0916 -163.555 -16.7453 0.075 2059 -2061 2 -17.7938 -165.682 -18.165 0.075 2060 -2062 2 -18.9093 -168.364 -19.1121 0.075 2061 -2063 2 -20.304 -170.951 -19.1627 0.075 2062 -2064 2 -22.0344 -173.352 -18.3651 0.075 2063 -2065 2 -23.8359 -175.467 -17.0536 0.075 2064 -2066 2 -26.0452 -177.396 -16.2699 0.075 2065 -2067 2 -28.5748 -179.015 -15.7938 0.075 2066 -2068 2 -31.4579 -180.031 -15.49 0.075 2067 -2069 2 -34.4463 -180.64 -15.1643 0.075 2068 -2070 2 -37.4326 -181.252 -14.7946 0.075 2069 -2071 2 -40.392 -181.956 -14.3685 0.075 2070 -2072 2 -43.3006 -182.323 -13.4498 0.075 2071 -2073 2 -46.0969 -182.868 -13.851 0.075 2072 -2074 2 -48.766 -183.586 -15.178 0.075 2073 -2075 2 -51.2427 -184.502 -16.7477 0.075 2074 -2076 2 -54.1406 -185.247 -17.1695 0.075 2075 -2077 2 -57.1539 -185.765 -17.1971 0.075 2076 -2078 2 -60.2093 -186.017 -16.9999 0.075 2077 -2079 2 9.41891 -138.975 4.26401 0.075 2046 -2080 2 7.74647 -135.087 2.91079 0.075 2079 -2081 2 5.74715 -131.153 2.30574 0.075 2080 -2082 2 3.96345 -131.586 -0.170751 0.075 2081 -2083 2 1.29063 -131.138 -1.64818 0.075 2082 -2084 2 -1.1486 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155.988 202.566 0.075 3237 -3239 2 81.011 158.823 202.932 0.075 3238 -3240 2 80.0401 161.737 203.202 0.075 3239 -3241 2 79.2335 164.694 203.572 0.075 3240 -3242 2 78.5032 167.593 204.336 0.075 3241 -3243 2 77.8668 170.567 204.651 0.075 3242 -3244 2 77.244 173.587 204.594 0.075 3243 -3245 2 76.3779 176.549 204.497 0.075 3244 -3246 2 75.1681 179.382 204.425 0.075 3245 -3247 2 74.149 182.274 204.32 0.075 3246 -3248 2 73.5074 185.289 204.162 0.075 3247 -3249 2 72.659 188.255 204.054 0.075 3248 -3250 2 71.7264 191.191 204.16 0.075 3249 -3251 2 70.82 194.126 204.426 0.075 3250 -3252 2 70.1135 197.107 204.808 0.075 3251 -3253 2 69.5962 199.996 205.741 0.075 3252 -3254 2 69.3311 202.647 207.201 0.075 3253 -3255 2 69.2125 205.093 209.069 0.075 3254 -3256 2 68.589 207.623 210.724 0.075 3255 -3257 2 67.6515 210.433 211.262 0.075 3256 -3258 2 66.6748 213.351 211.248 0.075 3257 -3259 2 65.7991 216.295 210.939 0.075 3258 -3260 2 66.8294 217.161 208.05 0.075 3259 -3261 2 67.9149 217.938 205.157 0.075 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223.873 208.114 0.075 3284 -3286 2 60.844 226.738 208.117 0.075 3285 -3287 2 59.5793 229.453 208.891 0.075 3286 -3288 2 58.7046 232.307 209.694 0.075 3287 -3289 2 57.7579 235.166 210.388 0.075 3288 -3290 2 56.6575 237.997 210.95 0.075 3289 -3291 2 55.2626 240.735 211.314 0.075 3290 -3292 2 54.0881 243.402 212.296 0.075 3291 -3293 2 53.2676 246.023 213.655 0.075 3292 -3294 2 52.8693 248.621 215.28 0.075 3293 -3295 2 52.7819 251.48 216.459 0.075 3294 -3296 2 52.1953 254.279 217.587 0.075 3295 -3297 2 51.2272 256.989 218.702 0.075 3296 -3298 2 49.9598 259.573 219.837 0.075 3297 -3299 2 48.5334 262.209 220.595 0.075 3298 -3300 2 47.0824 264.899 220.806 0.075 3299 -3301 2 45.7111 267.662 220.665 0.075 3300 -3302 2 44.6544 270.568 220.551 0.075 3301 -3303 2 43.6124 273.466 220.797 0.075 3302 -3304 2 42.3413 276.031 221.705 0.075 3303 -3305 2 40.8179 278.224 223.266 0.075 3304 -3306 2 39.2388 280.246 224.995 0.075 3305 -3307 2 37.8261 282.427 226.668 0.075 3306 -3308 2 36.3265 284.696 228.096 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126.547 0.075 3400 -3402 2 68.4191 9.84724 125.989 0.075 3401 -3403 2 67.8799 12.8409 125.196 0.075 3402 -3404 2 67.4594 15.7826 124.17 0.075 3403 -3405 2 66.8613 18.6818 123.129 0.075 3404 -3406 2 66.0838 21.5379 122.07 0.075 3405 -3407 2 64.5673 23.9336 120.716 0.075 3406 -3408 2 62.81 25.8944 119.09 0.075 3407 -3409 2 60.8233 27.4325 117.201 0.075 3408 -3410 2 58.8366 28.9706 115.311 0.075 3409 -3411 2 56.8251 29.7967 113.181 0.075 3410 -3412 2 54.7868 29.8514 110.788 0.075 3411 -3413 2 58.5078 -158.117 37.3782 0.075 2947 -3414 2 59.4009 -156.224 34.4966 0.075 3413 -3415 2 60.0242 -153.944 31.8638 0.075 3414 -3416 2 60.4477 -151.189 29.85 0.075 3415 -3417 2 60.7508 -147.688 29.2729 0.075 3416 -3418 2 61.2615 -144.222 28.6358 0.075 3417 -3419 2 62.2009 -140.94 27.7909 0.075 3418 -3420 2 63.7062 -137.917 26.6588 0.075 3419 -3421 2 64.3841 -134.627 26.9345 0.075 3420 -3422 2 65.3231 -131.414 26.6354 0.075 3421 -3423 2 66.2899 -128.21 26.2359 0.075 3422 -3424 2 67.1802 -124.981 25.858 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2 67.5085 -25.5827 13.3859 0.075 4482 -4484 2 68.5455 -22.6456 13.7836 0.075 4483 -4485 2 69.3649 -19.5909 13.8338 0.075 4484 -4486 2 69.583 -16.4415 13.6991 0.075 4485 -4487 2 69.8012 -13.2881 13.6388 0.075 4486 -4488 2 70.056 -10.1355 13.6788 0.075 4487 -4489 2 70.7816 -7.06926 13.428 0.075 4488 -4490 2 71.6836 -4.06916 13.0499 0.075 4489 -4491 2 72.7656 -1.14106 12.5396 0.075 4490 -4492 2 73.4296 1.78102 11.5328 0.075 4491 -4493 2 73.9421 4.73463 10.5351 0.075 4492 -4494 2 74.3011 7.72313 9.56304 0.075 4493 -4495 2 74.0208 10.8221 9.00241 0.075 4494 -4496 2 73.7472 13.9351 8.51899 0.075 4495 -4497 2 73.4897 17.0609 8.10895 0.075 4496 -4498 2 70.1897 20.4709 7.89562 0.075 4497 -4499 2 69.9987 23.6045 7.50857 0.075 4498 -4500 2 68.8214 26.4184 6.66438 0.075 4499 -4501 2 67.3071 29.0227 5.74393 0.075 4500 -4502 2 65.4525 31.3426 4.72008 0.075 4501 -4503 2 63.1844 33.2008 3.52872 0.075 4502 -4504 2 61.4784 34.2691 1.13303 0.075 4503 -4505 2 59.9775 35.1431 -1.50454 0.075 4504 -4506 2 58.5975 35.9218 -4.24417 0.075 4505 -4507 2 57.2175 36.7006 -6.98377 0.075 4506 -4508 2 55.247 37.6993 -9.20845 0.075 4507 -4509 2 52.9199 38.7797 -11.0291 0.075 4508 -4510 2 50.4164 39.944 -12.5582 0.075 4509 -4511 2 48.3277 41.7092 -14.1513 0.075 4510 -4512 2 46.1364 43.265 -15.8157 0.075 4511 -4513 2 43.8389 44.7634 -17.3807 0.075 4512 -4514 2 41.4083 46.3166 -18.683 0.075 4513 -4515 2 40.0624 47.4752 -22.1059 0.075 4514 -4516 2 38.4307 48.951 -25.2766 0.075 4515 -4517 2 36.8388 50.2309 -28.548 0.075 4516 -4518 2 34.8946 51.4876 -31.5618 0.075 4517 -4519 2 32.2152 52.8491 -33.9827 0.075 4518 -4520 2 33.8511 55.3211 -35.8279 0.075 4519 -4521 2 35.6116 56.7949 -38.4709 0.075 4520 -4522 2 37.2907 59.088 -40.3117 0.075 4521 -4523 2 38.9059 61.8808 -41.671 0.075 4522 -4524 2 40.4673 64.7066 -43.0252 0.075 4523 -4525 2 41.1139 67.4498 -45.0291 0.075 4524 -4526 2 40.91 70.2412 -46.9613 0.075 4525 -4527 2 39.7498 73.1348 -48.5545 0.075 4526 -4528 2 29.71 54.9356 -33.6758 0.075 4519 -4529 2 27.9219 57.561 -34.5003 0.075 4528 -4530 2 26.7797 59.9367 -36.2428 0.075 4529 -4531 2 26.2043 62.1489 -38.5744 0.075 4530 -4532 2 26.183 64.3154 -41.0393 0.075 4531 -4533 2 25.5572 66.008 -43.7645 0.075 4532 -4534 2 24.3644 67.7914 -46.167 0.075 4533 -4535 2 22.5763 69.7829 -47.97 0.075 4534 -4536 2 20.2713 72.0453 -48.5518 0.075 4535 -4537 2 18.2117 74.4742 -48.0522 0.075 4536 -4538 2 16.4326 76.8554 -46.7993 0.075 4537 -4539 2 14.9739 79.085 -44.8833 0.075 4538 -4540 2 39.2395 46.327 -16.1759 0.0362231 4514 -4541 2 36.0169 45.7915 -15.8302 0.035 4540 -4542 2 32.8938 44.8345 -15.5092 0.035 4541 -4543 2 29.9295 43.3987 -15.1338 0.035 4542 -4544 2 26.8142 42.3187 -14.798 0.035 4543 -4545 2 23.7059 41.2377 -14.4045 0.035 4544 -4546 2 20.6122 40.141 -13.9404 0.035 4545 -4547 2 17.8276 38.7967 -12.7613 0.035 4546 -4548 2 15.5572 37.7247 -10.8034 0.035 4547 -4549 2 13.7711 36.9323 -8.12544 0.035 4548 -4550 2 67.8739 21.3376 10.0064 0.075 4498 -4551 2 64.8095 22.101 10.4448 0.075 4550 -4552 2 61.672 22.917 10.6576 0.075 4551 -4553 2 58.582 23.9179 10.7994 0.075 4552 -4554 2 55.4747 24.8667 10.9063 0.075 4553 -4555 2 52.3572 25.7851 10.9929 0.075 4554 -4556 2 74.2849 19.8097 6.48986 0.075 4497 -4557 2 74.7229 22.3415 4.4407 0.075 4556 -4558 2 75.652 25.0131 2.94148 0.075 4557 -4559 2 77.0611 27.8239 1.98058 0.075 4558 -4560 2 78.6619 30.5273 2.94852 0.075 4559 -4561 2 80.2222 33.2268 3.98834 0.075 4560 -4562 2 81.7424 35.9225 5.09821 0.075 4561 -4563 2 83.6533 38.5954 5.0916 0.075 4562 -4564 2 85.911 40.9296 4.93482 0.075 4563 -4565 2 88.4944 42.9426 4.64589 0.075 4564 -4566 2 91.2844 40.4226 4.77923 0.075 4565 -4567 2 89.7094 38.0746 3.7758 0.075 4566 -4568 2 87.0122 36.5859 3.82429 0.075 4567 -4569 2 84.3075 35.144 3.5711 0.075 4568 -4570 2 81.8299 33.5705 2.8635 0.075 4569 -4571 2 79.8008 31.7073 1.48366 0.075 4570 -4572 2 78.0424 29.4843 0.280234 0.075 4571 -4573 2 76.1324 27.2075 -0.208681 0.075 4572 -4574 2 74.1393 24.8644 -0.114397 0.075 4573 -4575 2 72.4067 22.324 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15.4975 0.075 4642 -4644 2 145.538 21.2289 15.8186 0.075 4643 -4645 2 148.142 19.5646 16.1016 0.075 4644 -4646 2 150.798 17.9862 16.3909 0.075 4645 -4647 2 153.146 15.9579 16.4967 0.075 4646 -4648 2 155.646 14.1565 16.3122 0.075 4647 -4649 2 158.188 12.6196 16.4541 0.075 4648 -4650 2 160.56 11.3737 18.0224 0.075 4649 -4651 2 162.932 10.1279 19.5908 0.075 4650 -4652 2 165.445 9.01119 21.0014 0.075 4651 -4653 2 168.16 8.08044 22.1848 0.075 4652 -4654 2 167.885 6.21598 25.2353 0.075 4653 -4655 2 167.374 3.44285 27.4223 0.075 4654 -4656 2 167.042 0.445538 29.3302 0.075 4655 -4657 2 166.933 -2.72758 30.9966 0.075 4656 -4658 2 165.783 -7.97758 32.3166 0.075 4657 -4659 2 168.022 -4.52486 28.334 0.075 4657 -4660 2 169.925 -7.28571 28.2775 0.075 4659 -4661 2 171.986 -9.97456 28.3644 0.075 4660 -4662 2 174.122 -12.605 28.4849 0.075 4661 -4663 2 176.112 -15.3516 28.5094 0.075 4662 -4664 2 177.306 -18.246 27.5081 0.075 4663 -4665 2 178.173 -21.2483 26.2075 0.075 4664 -4666 2 179.044 -24.3801 25.239 0.075 4665 -4667 2 180.009 -27.5206 25.7853 0.075 4666 -4668 2 180.803 -30.6308 26.8266 0.075 4667 -4669 2 181.339 -33.6974 28.1731 0.075 4668 -4670 2 170.173 7.89373 24.4718 0.075 4653 -4671 2 173.052 7.49999 25.4137 0.075 4670 -4672 2 175.899 7.16017 26.463 0.075 4671 -4673 2 178.443 6.88622 27.9445 0.075 4672 -4674 2 180.243 6.71126 30.4065 0.075 4673 -4675 2 182.115 6.48158 32.8089 0.075 4674 -4676 2 184.475 5.9756 34.4234 0.075 4675 -4677 2 187.302 5.13954 35.1445 0.075 4676 -4678 2 190.099 3.91241 35.1151 0.075 4677 -4679 2 192.733 2.39256 35.2919 0.075 4678 -4680 2 195.359 0.88579 35.6404 0.075 4679 -4681 2 198.065 -0.42795 36.1709 0.075 4680 -4682 2 201.032 -0.385156 36.8605 0.075 4681 -4683 2 203.888 -0.519348 37.8823 0.075 4682 -4684 2 206.461 -0.972725 39.363 0.075 4683 -4685 2 208.53 -1.85954 41.4274 0.075 4684 -4686 2 210.6 -2.74637 43.4918 0.075 4685 -4687 2 213.017 -3.5881 45.035 0.075 4686 -4688 2 215.817 -4.28229 45.9902 0.075 4687 -4689 2 218.822 -4.35574 46.5338 0.075 4688 -4690 2 221.8 -4.4015 47.2054 0.075 4689 -4691 2 224.786 -4.31962 47.8068 0.075 4690 -4692 2 227.8 -4.03145 48.2115 0.075 4691 -4693 2 230.828 -3.63724 48.2834 0.075 4692 -4694 2 233.856 -3.24352 48.3706 0.075 4693 -4695 2 236.887 -2.92513 48.5034 0.075 4694 -4696 2 239.93 -2.86832 48.7612 0.075 4695 -4697 2 242.896 -3.41278 49.172 0.075 4696 -4698 2 245.542 -4.16704 50.2568 0.075 4697 -4699 2 247.772 -5.05326 52.1314 0.075 4698 -4700 2 249.591 -6.06121 54.3684 0.075 4699 -4701 2 251.519 -7.69658 56.0176 0.075 4700 -4702 2 253.57 -9.65219 56.9972 0.075 4701 -4703 2 255.75 -11.7609 57.3421 0.075 4702 -4704 2 258.203 -13.576 57.4737 0.075 4703 -4705 2 260.537 -15.4272 58.0608 0.075 4704 -4706 2 262.648 -17.3895 59.0033 0.075 4705 -4707 2 264.329 -19.5942 60.2848 0.075 4706 -4708 2 265.355 -21.6953 62.2286 0.075 4707 -4709 2 266.94 -23.6994 63.7143 0.075 4708 -4710 2 269.335 -25.0824 64.3657 0.075 4709 -4711 2 272.376 -25.0903 64.083 0.075 4710 -4712 2 275.399 -25.2852 63.6943 0.075 4711 -4713 2 278.409 -25.6311 63.3338 0.075 4712 -4714 2 281.416 -26.0632 63.0269 0.075 4713 -4715 2 284.459 -26.2444 62.8339 0.075 4714 -4716 2 287.477 -25.9412 62.6287 0.075 4715 -4717 2 290.496 -25.5334 62.4205 0.075 4716 -4718 2 293.531 -25.2518 62.2146 0.075 4717 -4719 2 296.578 -25.2815 62.0229 0.075 4718 -4720 2 299.567 -25.2231 61.4823 0.075 4719 -4721 2 302.513 -25.1602 60.6924 0.075 4720 -4722 2 305.473 -25.39 59.9731 0.075 4721 -4723 2 308.404 -26.1238 60.0594 0.075 4722 -4724 2 311.221 -27.1928 60.4601 0.075 4723 -4725 2 313.921 -28.462 60.8756 0.075 4724 -4726 2 316.677 -29.1544 59.7543 0.075 4725 -4727 2 319.394 -29.4701 58.4175 0.075 4726 -4728 2 322.23 -29.621 57.5036 0.075 4727 -4729 2 325.253 -29.7097 57.4714 0.075 4728 -4730 2 327.463 -30.2402 59.5125 0.075 4729 -4731 2 328.763 -31.0089 62.0946 0.075 4730 -4732 2 329.769 -31.8548 64.8521 0.075 4731 -4733 2 330.774 -32.7008 67.6096 0.075 4732 -4734 2 332.031 -34.2694 69.9045 0.075 4733 -4735 2 333.463 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109.965 211.346 -45.1887 0.075 4803 -4805 2 110.482 214.142 -46.2391 0.075 4804 -4806 2 110.763 217.076 -46.8886 0.075 4805 -4807 2 110.772 220.055 -47.0528 0.075 4806 -4808 2 110.43 223.03 -46.581 0.075 4807 -4809 2 109.394 225.862 -46.3117 0.075 4808 -4810 2 108.572 228.454 -47.2147 0.075 4809 -4811 2 108.142 230.946 -48.8148 0.075 4810 -4812 2 108.574 233.648 -50.1197 0.075 4811 -4813 2 108.826 236.578 -50.7702 0.075 4812 -4814 2 108.711 239.536 -51.2995 0.075 4813 -4815 2 108.141 242.458 -51.8654 0.075 4814 -4816 2 107.229 245.282 -52.4859 0.075 4815 -4817 2 106.027 247.94 -53.259 0.075 4816 -4818 2 104.59 250.439 -54.1775 0.075 4817 -4819 2 102.945 252.736 -55.2747 0.075 4818 -4820 2 101.076 254.969 -56.1033 0.075 4819 -4821 2 99.2741 257.308 -56.7493 0.075 4820 -4822 2 97.6045 259.789 -57.2472 0.075 4821 -4823 2 95.9913 262.266 -57.9174 0.075 4822 -4824 2 94.4312 264.75 -58.6774 0.075 4823 -4825 2 92.9471 267.248 -59.5334 0.075 4824 -4826 2 91.5977 269.774 -60.5277 0.075 4825 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1759 -1761 2 16.1309 -49.1042 -12.1983 0.075 1760 -1762 2 17.5989 -45.999 -13.2599 0.075 1761 -1763 2 20.9459 -46.5244 -17.3777 0.075 1762 -1764 2 15.6528 -43.3845 -12.5947 0.075 1762 -1765 2 14.1891 -40.9139 -11.0656 0.075 1764 -1766 2 14.383 -38.3593 -8.97058 0.075 1765 -1767 2 13.8587 -35.9555 -6.79229 0.075 1766 -1768 2 13.0858 -33.7726 -4.41731 0.075 1767 -1769 2 16.2274 -36.2082 -5.67748 0.075 1768 -1770 2 19.3928 -38.073 -2.51975 0.075 1769 -1771 2 20.2875 -39.72 -5.05727 0.075 1770 -1772 2 22.2716 -41.5405 -6.20911 0.075 1771 -1773 2 24.3798 -43.2785 -4.74384 0.075 1772 -1774 2 26.2161 -45.5734 -3.72648 0.075 1773 -1775 2 28.2578 -47.9729 -3.54015 0.075 1774 -1776 2 30.7195 -49.927 -3.30669 0.075 1775 -1777 2 33.4116 -51.5354 -3.10019 0.075 1776 -1778 2 35.8668 -53.4605 -2.72482 0.075 1777 -1779 2 38.3984 -54.9748 -1.60182 0.075 1778 -1780 2 40.4713 -57.0993 -0.605459 0.075 1779 -1781 2 42.5445 -59.426 -0.198714 0.075 1780 -1782 2 45.1473 -61.106 -0.506266 0.075 1781 -1783 2 48.1906 -59.5426 -2.31023 0.075 1782 -1784 2 51.0056 -58.087 -1.00746 0.075 1783 -1785 2 54.1163 -56.5381 -1.40458 0.075 1784 -1786 2 57.3951 -55.3586 -1.70699 0.075 1785 -1787 2 60.4804 -53.762 -1.99486 0.075 1786 -1788 2 63.2586 -51.752 -2.64559 0.075 1787 -1789 2 53.9762 -59.275 -1.07235 0.075 1784 -1790 2 57.1118 -59.8089 -1.17676 0.075 1789 -1791 2 60.0486 -60.8708 -0.648987 0.075 1790 -1792 2 62.9433 -62.21 -0.552204 0.075 1791 -1793 2 65.8074 -63.5542 -0.128027 0.075 1792 -1794 2 68.3483 -64.3927 1.43039 0.075 1793 -1795 2 70.1836 -65.5088 3.76099 0.075 1794 -1796 2 72.8169 -66.9344 4.58671 0.075 1795 -1797 2 75.4898 -68.6431 4.22951 0.075 1796 -1798 2 78.2299 -69.5068 2.90352 0.075 1797 -1799 2 81.2021 -70.0476 1.9003 0.075 1798 -1800 2 84.3735 -70.2512 1.56017 0.075 1799 -1801 2 87.397 -71.4848 1.52307 0.075 1800 -1802 2 90.4661 -72.3384 1.06307 0.075 1801 -1803 2 93.3635 -73.2886 -0.0934076 0.075 1802 -1804 2 96.1212 -74.5381 -1.15345 0.075 1803 -1805 2 98.3416 -76.2641 -2.71229 0.075 1804 -1806 2 100.476 -76.6508 -5.10328 0.075 1805 -1807 2 89.5079 -67.8852 2.7473 0.075 1800 -1808 2 46.3891 -64.1321 -3.01387 0.075 1782 -1809 2 48.1442 -66.6121 -5.81118 0.075 1808 -1810 2 50.0707 -69.2258 -8.36339 0.075 1809 -1811 2 50.9377 -69.9766 -5.00141 0.075 1810 -1812 2 51.0333 -71.012 -1.60692 0.075 1811 -1813 2 51.6208 -72.1951 1.59187 0.075 1812 -1814 2 51.8751 -74.8863 3.78523 0.075 1813 -1815 2 52.9025 -75.9282 6.86366 0.075 1814 -1816 2 53.0616 -71.8593 -11.491 0.075 1810 -1817 2 56.004 -75.4466 -13.3508 0.075 1816 -1818 2 51.224 -75.5766 -16.0308 0.075 1817 -1819 2 58.6068 -77.4299 -13.5958 0.075 1817 -1820 2 60.0321 -80.1743 -14.2302 0.075 1819 -1821 2 61.15 -83.2117 -14.4534 0.075 1820 -1822 2 63.3021 -85.5038 -15.2305 0.075 1821 -1823 2 65.2011 -87.9204 -16.2736 0.075 1822 -1824 2 66.9632 -90.6528 -16.6295 0.075 1823 -1825 2 69.2456 -92.9196 -17.1112 0.075 1824 -1826 2 71.6477 -94.8666 -16.0757 0.075 1825 -1827 2 73.8552 -97.1693 -15.3787 0.075 1826 -1828 2 75.9967 -99.5854 -15.7011 0.075 1827 -1829 2 78.1647 -101.837 -16.6507 0.075 1828 -1830 2 81.0771 -103.296 -16.5527 0.075 1829 -1831 2 83.7135 -105.048 -17.0578 0.075 1830 -1832 2 85.6783 -107.479 -17.8437 0.075 1831 -1833 2 86.9957 -110.473 -17.5617 0.075 1832 -1834 2 22.1012 -36.1483 -2.86392 0.075 1770 -1835 2 25.1346 -35.4538 -3.29843 0.075 1834 -1836 2 28.1749 -36.3277 -3.62742 0.075 1835 -1837 2 29.965 -37.1149 -6.3704 0.075 1836 -1838 2 31.9595 -37.8788 -8.94075 0.075 1837 -1839 2 33.0776 -37.6032 -12.0713 0.075 1838 -1840 2 33.833 -36.385 -15.0654 0.075 1839 -1841 2 36.1514 -34.9172 -16.8231 0.075 1840 -1842 2 39.0822 -34.1314 -18.2303 0.075 1841 -1843 2 12.0514 -31.5063 -1.87564 0.075 1768 -1844 2 10.3264 -29.764 0.564483 0.075 1843 -1845 2 9.30579 -26.474 1.56933 0.075 1844 -1846 2 11.3726 -25.7224 -0.923363 0.075 1845 -1847 2 13.7557 -24.2101 -2.65372 0.075 1846 -1848 2 16.1792 -22.6942 -4.29032 0.075 1847 -1849 2 19.2468 -22.0977 -5.38786 0.075 1848 -1850 2 22.2571 -21.8783 -4.20821 0.075 1849 -1851 2 25.2866 -22.1939 -3.19551 0.075 1850 -1852 2 27.9577 -22.6473 -4.89726 0.075 1851 -1853 2 7.75398 -25.3337 5.05456 0.075 1845 -1854 2 5.31898 -22.884 7.08701 0.075 1853 -1855 2 4.0895 -19.7766 9.14589 0.075 1854 -1856 2 2.94343 -16.8992 11.7059 0.075 1855 -1857 2 34.0779 -67.4535 11.1172 0.075 1697 -1858 2 36.6393 -71.6576 11.4709 0.075 1857 -1859 2 35.0115 -73.5325 9.48445 0.075 1858 -1860 2 33.9438 -76.5136 9.39583 0.075 1859 -1861 2 33.3101 -79.5818 9.20543 0.075 1860 -1862 2 32.641 -82.1272 7.48483 0.075 1861 -1863 2 31.8702 -84.7693 5.92832 0.075 1862 -1864 2 31.1988 -87.8145 5.43786 0.075 1863 -1865 2 29.8432 -90.6375 4.92554 0.075 1864 -1866 2 28.5985 -93.544 4.65484 0.075 1865 -1867 2 41.3593 -76.4376 11.5909 0.075 1858 -1868 2 37.8693 -74.6276 16.0309 0.075 1867 -1869 2 43.9171 -79.1474 12.3568 0.075 1867 -1870 2 46.0516 -80.7093 15.1043 0.075 1869 -1871 2 49.0671 -82.0151 16.8431 0.075 1870 -1872 2 52.058 -84.4079 17.2351 0.075 1871 -1873 2 55.3899 -86.0332 18.2615 0.075 1872 -1874 2 59.2738 -84.7346 16.5795 0.075 1873 -1875 2 61.5605 -84.0388 12.8455 0.075 1874 -1876 2 57.3002 -88.1393 19.6589 0.075 1873 -1877 2 59.9646 -89.8686 19.6171 0.075 1876 -1878 2 62.5968 -91.1202 18.4769 0.075 1877 -1879 2 64.506 -92.119 16.172 0.075 1878 -1880 2 65.6175 -94.2174 14.2498 0.075 1879 -1881 2 66.4927 -96.8126 13.0605 0.075 1880 -1882 2 66.9441 -98.1524 10.2194 0.075 1881 -1883 2 67.8723 -99.5527 7.54095 0.075 1882 -1884 2 68.7924 -101.461 5.16907 0.075 1883 -1885 2 69.9564 -103.625 3.37132 0.075 1884 -1886 2 70.0064 -106.756 3.1088 0.075 1885 -1887 2 69.7812 -109.025 1.09259 0.075 1886 -1888 2 69.9209 -111.229 -1.02391 0.075 1887 -1889 2 71.6556 -113.627 -1.7077 0.075 1888 -1890 2 73.8725 -115.024 -3.28504 0.075 1889 -1891 2 26.7292 -51.4464 13.7928 0.075 1694 -1892 2 26.2169 -48.2492 14.1796 0.075 1891 -1893 2 26.0774 -44.9319 13.7711 0.075 1892 -1894 2 26.6002 -41.6762 13.3592 0.075 1893 -1895 2 28.0209 -38.6518 13.1115 0.075 1894 -1896 2 31.4076 -41.1255 9.02602 0.075 1895 -1897 2 32.2876 -37.2155 6.57262 0.075 1896 -1898 2 34.7076 -46.7155 9.61262 0.075 1896 -1899 2 34.7586 -49.8911 10.0902 0.075 1898 -1900 2 35.4817 -52.9906 10.4707 0.075 1899 -1901 2 36.8747 -55.8462 10.8514 0.075 1900 -1902 2 38.5339 -58.549 11.3472 0.075 1901 -1903 2 40.6024 -60.5594 9.97921 0.075 1902 -1904 2 43.2264 -62.1637 9.26204 0.075 1903 -1905 2 45.0908 -63.6197 7.23534 0.075 1904 -1906 2 46.8121 -64.8723 8.62096 0.075 1905 -1907 2 48.0831 -65.9983 5.95949 0.075 1906 -1908 2 49.4218 -67.5961 3.64105 0.075 1907 -1909 2 51.3817 -68.6506 1.40746 0.075 1908 -1910 2 53.5132 -70.093 -0.342109 0.075 1909 -1911 2 54.7629 -71.2353 -2.99605 0.075 1910 -1912 2 55.8925 -72.7471 -5.49486 0.075 1911 -1913 2 57.9178 -74.2794 -7.32064 0.075 1912 -1914 2 60.3589 -76.0037 -8.19637 0.075 1913 -1915 2 62.4846 -77.928 -9.48803 0.075 1914 -1916 2 65.0303 -79.5376 -10.4265 0.075 1915 -1917 2 67.5954 -80.787 -11.7091 0.075 1916 -1918 2 70.3686 -81.4443 -13.0627 0.075 1917 -1919 2 73.1962 -81.958 -14.3633 0.075 1918 -1920 2 45.808 -65.0408 5.18295 0.075 1906 -1921 2 43.8929 -64.908 2.32439 0.075 1920 -1922 2 43.899 -65.6681 -1.2191 0.075 1921 -1923 2 43.8163 -66.7423 -4.67476 0.075 1922 -1924 2 43.6936 -67.8644 -8.07417 0.075 1923 -1925 2 37.5446 -49.0453 9.87242 0.075 1898 -1926 2 39.5021 -52.1188 10.1953 0.075 1925 -1927 2 41.1508 -55.2251 9.99244 0.075 1926 -1928 2 41.5575 -56.473 6.56323 0.075 1927 -1929 2 42.0824 -58.8192 3.92288 0.075 1928 -1930 2 28.4123 -34.7867 12.5842 0.075 1895 -1931 2 29.3689 -31.005 12.8286 0.075 1930 -1932 2 30.4654 -27.6617 11.3483 0.075 1931 -1933 2 31.609 -28.8778 8.26393 0.075 1932 -1934 2 33.5807 -29.4939 5.47452 0.075 1933 -1935 2 36.3146 -29.5199 3.36792 0.075 1934 -1936 2 39.3781 -29.8431 1.89142 0.075 1935 -1937 2 42.1613 -30.9433 0.134281 0.075 1936 -1938 2 45.3929 -31.1745 -1.08455 0.075 1937 -1939 2 47.9596 -30.4093 -3.25119 0.075 1938 -1940 2 50.5674 -30.6867 -5.46714 0.075 1939 -1941 2 51.2024 -32.2082 -8.44051 0.075 1940 -1942 2 52.5262 -34.2973 -10.7719 0.075 1941 -1943 2 53.1881 -36.9954 -12.6184 0.075 1942 -1944 2 55.3302 -38.7616 -14.4674 0.075 1943 -1945 2 57.4375 -39.879 -16.8902 0.075 1944 -1946 2 53.678 -29.9129 -6.1651 0.075 1940 -1947 2 56.7996 -29.7042 -7.07894 0.075 1946 -1948 2 59.948 -29.0029 -7.67189 0.075 1947 -1949 2 62.6775 -27.5633 -7.70447 0.075 1948 -1950 2 64.9155 -25.8349 -6.14939 0.075 1949 -1951 2 67.69 -24.1833 -6.7249 0.075 1950 -1952 2 70.4028 -22.4519 -7.33049 0.075 1951 -1953 2 73.1954 -20.9909 -6.77623 0.075 1952 -1954 2 76.1848 -20.0567 -5.82166 0.075 1953 -1955 2 31.0867 -24.2701 10.0022 0.075 1932 -1956 2 31.1241 -20.8799 8.62538 0.075 1955 -1957 2 31.4066 -17.2499 7.97382 0.075 1956 -1958 2 32.1145 -13.6528 7.55128 0.075 1957 -1959 2 33.5272 -11.0039 9.08524 0.075 1958 -1960 2 34.6489 -7.88152 9.53515 0.075 1959 -1961 2 34.995 -4.54001 9.46184 0.075 1960 -1962 2 35.5819 -1.23702 9.30973 0.075 1961 -1963 2 36.5516 1.8819 8.54747 0.075 1962 -1964 2 36.8585 5.07754 7.58987 0.075 1963 -1965 2 36.5703 8.39908 7.09105 0.075 1964 -1966 2 33.2703 11.8091 6.87772 0.075 1965 -1967 2 33.008 15.1131 6.43954 0.075 1966 -1968 2 31.4727 17.9192 5.47934 0.075 1967 -1969 2 29.3614 19.5435 3.65313 0.075 1968 -1970 2 27.8578 20.4008 0.781317 0.075 1969 -1971 2 25.71 21.4574 -1.48323 0.075 1970 -1972 2 23.3122 23.0562 -3.15716 0.075 1971 -1973 2 20.8317 24.6634 -4.73039 0.075 1972 -1974 2 18.9442 26.312 -9.20597 0.075 1973 -1975 2 16.2048 28.0509 -13.0839 0.075 1974 -1976 2 17.8311 30.5149 -14.9146 0.075 1975 -1977 2 19.5271 32.5331 -17.0022 0.075 1976 -1978 2 20.8557 35.3145 -18.5183 0.075 1977 -1979 2 20.3302 38.1252 -20.3255 0.075 1978 -1980 2 13.4485 30.506 -12.8928 0.075 1975 -1981 2 12.219 33.2126 -14.8845 0.075 1980 -1982 2 11.6635 35.3474 -17.8389 0.075 1981 -1983 2 9.35624 37.7767 -19.1619 0.075 1982 -1984 2 7.40611 40.455 -17.5701 0.075 1983 -1985 2 17.9847 24.5705 -2.09265 0.035 1973 -1986 2 14.2184 23.2709 -1.68615 0.035 1985 -1987 2 10.448 21.938 -1.15592 0.035 1986 -1988 2 7.70887 20.6477 1.2721 0.035 1987 -1989 2 29.1454 13.1402 9.33227 0.075 1966 -1990 2 24.1628 14.6349 9.54048 0.075 1989 -1991 2 37.4673 11.7025 5.03405 0.075 1965 -1992 2 38.7839 14.9868 3.65032 0.075 1991 -1993 2 40.7008 18.2712 4.85812 0.075 1992 -1994 2 43.4656 21.0974 4.66077 0.075 1993 -1995 2 46.2556 18.5774 4.79411 0.075 1994 -1996 2 44.606 16.1882 3.79202 0.075 1995 -1997 2 41.8281 14.6961 3.59891 0.075 1996 -1998 2 39.8593 12.6233 2.26695 0.075 1997 -1999 2 37.8914 10.2047 2.15599 0.075 1998 -2000 2 36.4957 7.40231 2.00149 0.075 1999 -2001 2 35.7233 4.41204 1.43035 0.075 2000 -2002 2 35.7139 1.25478 1.437 0.075 2001 -2003 2 35.6292 -1.88544 1.19242 0.075 2002 -2004 2 35.6283 -4.7694 -0.0339671 0.075 2003 -2005 2 35.9012 -7.41892 -1.73784 0.075 2004 -2006 2 35.7978 -9.85919 -3.72398 0.075 2005 -2007 2 35.2367 -12.7784 -4.51812 0.075 2006 -2008 2 35.5668 -15.8753 -5.04479 0.075 2007 -2009 2 35.5676 -19.0124 -5.14442 0.075 2008 -2010 2 34.744 -22.0272 -5.41567 0.075 2009 -2011 2 34.4247 -24.836 -6.80235 0.075 2010 -2012 2 34.6263 -27.9321 -7.27065 0.075 2011 -2013 2 36.1003 -30.3296 -8.40598 0.075 2012 -2014 2 37.3059 -32.1157 -10.6916 0.075 2013 -2015 2 37.9362 -33.8569 -13.2221 0.075 2014 -2016 2 39.349 -36.3784 -14.1907 0.075 2015 -2017 2 40.8519 -39.1273 -13.8956 0.075 2016 -2018 2 41.3932 -42.1903 -13.4099 0.075 2017 -2019 2 52.2656 17.2074 4.56744 0.075 1995 -2020 2 55.9735 18.5179 4.16742 0.075 2019 -2021 2 59.7997 18.2563 3.95035 0.075 2020 -2022 2 56.8956 14.2174 4.64744 0.075 2019 -2023 2 58.2582 17.9503 4.08268 0.075 2022 -2024 2 60.2239 21.2344 2.99668 0.075 2023 -2025 2 58.5012 11.5402 4.78869 0.075 2022 -2026 2 61.2025 10.5591 5.81529 0.075 2025 -2027 2 63.9329 9.86678 7.20421 0.075 2026 -2028 2 66.9404 9.39993 8.02522 0.075 2027 -2029 2 69.9815 8.63532 8.25246 0.075 2028 -2030 2 73.0526 8.25322 8.86525 0.075 2029 -2031 2 75.7796 6.78574 9.44825 0.075 2030 -2032 2 78.4545 5.09871 9.73945 0.075 2031 -2033 2 80.967 3.18574 9.68797 0.075 2032 -2034 2 83.4452 1.82425 10.8692 0.075 2033 -2035 2 86.0801 0.73956 12.2418 0.075 2034 -2036 2 85.737 -1.51926 15.7029 0.075 2035 -2037 2 85.3865 -5.03242 17.8834 0.075 2036 -2038 2 84.2365 -10.2824 19.2034 0.075 2037 -2039 2 86.5672 -6.9692 15.1428 0.075 2037 -2040 2 88.7254 -9.8412 15.2222 0.075 2039 -2041 2 90.4911 -12.8051 14.7909 0.075 2040 -2042 2 91.4315 -16.0763 13.9172 0.075 2041 -2043 2 92.2009 -19.3626 15.1069 0.075 2042 -2044 2 88.1568 0.544093 14.5498 0.075 2035 -2045 2 91.0769 0.180278 15.5897 0.075 2044 -2046 2 93.0645 -0.033838 17.9239 0.075 2045 -2047 2 95.6826 -0.739178 19.0972 0.075 2046 -2048 2 98.4162 -2.21919 19.2261 0.075 2047 -2049 2 101.291 -2.99453 19.8308 0.075 2048 -2050 2 103.964 -3.38663 21.2356 0.075 2049 -2051 2 106.156 -4.28322 23.2315 0.075 2050 -2052 2 109.115 -4.70013 24.0138 0.075 2051 -2053 2 112.169 -4.61541 24.6183 0.075 2052 -2054 2 115.262 -4.23401 24.7645 0.075 2053 -2055 2 118.361 -4.0936 24.9792 0.075 2054 -2056 2 121.059 -4.86688 26.104 0.075 2055 -2057 2 123.003 -6.17952 28.0878 0.075 2056 -2058 2 125.235 -8.22082 28.6386 0.075 2057 -2059 2 127.599 -10.1275 29.2355 0.075 2058 -2060 2 128.967 -12.3263 30.8987 0.075 2059 -2061 2 131.374 -13.5806 31.5496 0.075 2060 -2062 2 134.462 -13.8122 31.1821 0.075 2061 -2063 2 137.555 -14.0734 30.9347 0.075 2062 -2064 2 140.644 -13.6798 30.7223 0.075 2063 -2065 2 143.726 -13.6302 30.3364 0.075 2064 -2066 2 146.741 -13.8566 29.7264 0.075 2065 -2067 2 149.594 -15.0028 30.1666 0.075 2066 -2068 2 152.388 -15.4848 28.8913 0.075 2067 -2069 2 155.2 -15.7183 29.271 0.075 2068 -2070 2 156.514 -16.5078 31.9193 0.075 2069 -2071 2 157.717 -17.8871 34.3639 0.075 2070 -2072 2 159.213 -20.3305 35.6016 0.075 2071 -2073 2 160.745 -22.451 37.2347 0.075 2072 -2074 2 162.288 -24.1012 39.3882 0.075 2073 -2075 2 163.739 -26.3283 40.9226 0.075 2074 -2076 2 45.1627 26.7512 2.68234 0.075 1994 -2077 2 44.4456 29.6588 3.39753 0.075 2076 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34.1479 0.075 1862 -1864 2 63.181 -179.909 34.454 0.075 1863 -1865 2 64.8105 -182.454 34.9875 0.075 1864 -1866 2 66.5453 -184.783 35.9937 0.075 1865 -1867 2 68.3277 -186.846 37.3654 0.075 1866 -1868 2 69.1883 -189.258 39.0689 0.075 1867 -1869 2 69.9652 -191.701 40.7812 0.075 1868 -1870 2 70.6687 -193.886 42.8028 0.075 1869 -1871 2 71.7626 -195.981 44.7265 0.075 1870 -1872 2 73.4304 -198.321 45.7929 0.075 1871 -1873 2 75.4669 -200.613 46.0827 0.075 1872 -1874 2 77.7096 -202.687 46.4725 0.075 1873 -1875 2 80.0116 -204.632 47.1207 0.075 1874 -1876 2 82.4716 -206.193 48.1155 0.075 1875 -1877 2 84.8303 -207.876 49.1421 0.075 1876 -1878 2 87.0137 -209.798 50.148 0.075 1877 -1879 2 89.0233 -212.106 50.4965 0.075 1878 -1880 2 91.9234 -213.11 50.4698 0.075 1879 -1881 2 94.9714 -213.506 50.7021 0.075 1880 -1882 2 97.921 -213.203 51.4071 0.075 1881 -1883 2 100.819 -212.991 52.3707 0.075 1882 -1884 2 103.758 -213.551 53.0533 0.075 1883 -1885 2 106.681 -214.089 53.7997 0.075 1884 -1886 2 108.92 -215.178 55.6148 0.075 1885 -1887 2 111.087 -216.338 57.4724 0.075 1886 -1888 2 113.446 -217.102 59.3028 0.075 1887 -1889 2 116.034 -217.694 60.8201 0.075 1888 -1890 2 118.874 -218.131 61.9239 0.075 1889 -1891 2 121.171 -218.221 63.4944 0.075 1890 -1892 2 122.07 -217.9 66.4133 0.075 1891 -1893 2 51.7237 -150.846 24.2375 0.075 1349 -1894 2 54.5459 -152.737 24.8602 0.075 1893 -1895 2 57.5622 -153.649 25.4262 0.075 1894 -1896 2 60.4483 -153.405 26.768 0.075 1895 -1897 2 63.8326 -153.971 26.3205 0.075 1896 -1898 2 67.2785 -153.921 26.2526 0.075 1897 -1899 2 70.6817 -153.441 26.3726 0.075 1898 -1900 2 73.95 -153.29 27.4267 0.075 1899 -1901 2 74.2394 -154.986 30.0471 0.075 1900 -1902 2 74.6745 -156.897 32.4737 0.075 1901 -1903 2 75.2788 -158.987 34.7264 0.075 1902 -1904 2 76.575 -161.213 36.4086 0.075 1903 -1905 2 78.0734 -163.46 37.9776 0.075 1904 -1906 2 79.2894 -165.787 39.6768 0.075 1905 -1907 2 80.3595 -168.042 41.4213 0.075 1906 -1908 2 80.9773 -169.24 44.1749 0.075 1907 -1909 2 81.3846 -170.152 47.1464 0.075 1908 -1910 2 82.1006 -171.618 49.7682 0.075 1909 -1911 2 82.652 -173.283 52.2308 0.075 1910 -1912 2 82.84 -174.25 55.2 0.075 1911 -1913 2 76.8492 -152.28 25.0512 0.075 1900 -1914 2 79.5186 -150.708 22.6592 0.075 1913 -1915 2 82.04 -149.26 20.0267 0.075 1914 -1916 2 81.7129 -153.556 20.106 0.075 1915 -1917 2 81.4853 -157.46 21.2007 0.075 1916 -1918 2 81.77 -159.54 24.9333 0.075 1917 -1919 2 84.158 -147.107 19.2238 0.075 1915 -1920 2 85.8695 -145.037 17.5338 0.075 1919 -1921 2 88.5009 -143.596 18.0729 0.075 1920 -1922 2 91.3457 -142.758 19.1899 0.075 1921 -1923 2 94.057 -141.905 20.5652 0.075 1922 -1924 2 96.5927 -141.784 22.4331 0.075 1923 -1925 2 98.6357 -141.777 21.7531 0.075 1924 -1926 2 99.1769 -140.818 18.8137 0.075 1925 -1927 2 100.774 -139.912 16.4344 0.075 1926 -1928 2 103.766 -139.286 15.8472 0.075 1927 -1929 2 106.621 -137.95 16.0329 0.075 1928 -1930 2 108.735 -136.726 17.9031 0.075 1929 -1931 2 111.16 -135.9 19.56 0.075 1930 -1932 2 113.451 -137.38 20.5556 0.075 1931 -1933 2 115.031 -139.741 21.6485 0.075 1932 -1934 2 116.159 -142.57 22.0109 0.075 1933 -1935 2 117.274 -145.399 22.4085 0.075 1934 -1936 2 118.482 -147.967 23.1342 0.075 1935 -1937 2 119.383 -149.467 20.6107 0.075 1936 -1938 2 120.283 -150.967 18.0872 0.075 1937 -1939 2 121.093 -152.941 16.1839 0.075 1938 -1940 2 121.936 -155.818 15.5501 0.075 1939 -1941 2 123.333 -158.274 14.5518 0.075 1940 -1942 2 124.749 -160.313 12.7603 0.075 1941 -1943 2 126.082 -162.371 10.9158 0.075 1942 -1944 2 127.534 -164.634 9.46021 0.075 1943 -1945 2 129.201 -166.912 8.25263 0.075 1944 -1946 2 130.978 -169.134 7.11074 0.075 1945 -1947 2 132.672 -171.205 5.62064 0.075 1946 -1948 2 133.629 -173.889 4.63161 0.075 1947 -1949 2 134.692 -176.751 4.84525 0.075 1948 -1950 2 136.253 -179.325 5.23669 0.075 1949 -1951 2 139.109 -180.245 4.67024 0.075 1950 -1952 2 141.876 -181.182 3.7287 0.075 1951 -1953 2 144.681 -182.303 3.51865 0.075 1952 -1954 2 147.324 -183.468 4.54506 0.075 1953 -1955 2 150.07 -184.66 5.05333 0.075 1954 -1956 2 113.371 -133.621 19.1406 0.075 1931 -1957 2 116.258 -132.087 18.7601 0.075 1956 -1958 2 118.743 -129.888 18.3214 0.075 1957 -1959 2 121.439 -127.939 17.9174 0.075 1958 -1960 2 124.503 -127.171 17.1858 0.075 1959 -1961 2 126.82 -125.04 16.44 0.075 1960 -1962 2 127.771 -123.622 19.0823 0.075 1961 -1963 2 129.217 -121.619 20.9836 0.075 1962 -1964 2 130.776 -119.68 22.8945 0.075 1963 -1965 2 132.76 -118.197 24.8098 0.075 1964 -1966 2 134.05 -116.241 26.8603 0.075 1965 -1967 2 134.481 -113.982 28.9478 0.075 1966 -1968 2 134.754 -111.474 30.8171 0.075 1967 -1969 2 135.097 -108.68 32.1248 0.075 1968 -1970 2 135.245 -105.771 33.3003 0.075 1969 -1971 2 136.075 -103.225 34.7475 0.075 1970 -1972 2 137.721 -101.537 36.8217 0.075 1971 -1973 2 139.169 -99.8642 38.9663 0.075 1972 -1974 2 140.023 -98.3674 41.5983 0.075 1973 -1975 2 140.988 -96.5497 43.7521 0.075 1974 -1976 2 142.317 -94.0187 44.6866 0.075 1975 -1977 2 144.079 -91.9014 46.206 0.075 1976 -1978 2 145.205 -89.3173 46.8257 0.075 1977 -1979 2 145.329 -86.2015 46.8461 0.075 1978 -1980 2 145.266 -83.3128 47.8116 0.075 1979 -1981 2 145.331 -81.1619 50.05 0.075 1980 -1982 2 145.236 -80.2881 47.6726 0.075 1981 -1983 2 145.106 -79.5364 44.6204 0.075 1982 -1984 2 144.85 -78.4618 41.7648 0.075 1983 -1985 2 144.199 -76.4441 39.4432 0.075 1984 -1986 2 143.296 -73.8445 37.9225 0.075 1985 -1987 2 142.339 -71.0032 37.0682 0.075 1986 -1988 2 141.393 -68.1376 36.1885 0.075 1987 -1989 2 140.362 -65.5226 34.8173 0.075 1988 -1990 2 139.19 -63.16 33.1067 0.075 1989 -1991 2 139.586 -60.2938 34.8884 0.075 1990 -1992 2 138.237 -57.8984 33.2723 0.075 1991 -1993 2 137.117 -55.4581 31.2076 0.075 1992 -1994 2 136.256 -53.442 28.6337 0.075 1993 -1995 2 135.306 -51.2601 26.2292 0.075 1994 -1996 2 134.631 -49.0255 23.7961 0.075 1995 -1997 2 134.03 -46.69 21.4933 0.075 1996 -1998 2 139.257 -63.2432 36.1809 0.075 1990 -1999 2 139.323 -63.3264 39.2551 0.075 1998 -2000 2 139.39 -63.4095 42.3293 0.075 1999 -2001 2 137.591 -65.6782 43.3183 0.075 2000 -2002 2 135.76 -67.0277 45.2026 0.075 2001 -2003 2 134.377 -67.8628 47.8194 0.075 2002 -2004 2 132.71 -68.6929 50.2221 0.075 2003 -2005 2 130.239 -69.8416 51.4581 0.075 2004 -2006 2 127.525 -71.1759 52.0072 0.075 2005 -2007 2 125.782 -72.2079 54.1104 0.075 2006 -2008 2 124.935 -73.0556 56.9246 0.075 2007 -2009 2 124.546 -74.2363 59.6381 0.075 2008 -2010 2 124.924 -76.2456 61.8926 0.075 2009 -2011 2 126.265 -78.9207 62.5796 0.075 2010 -2012 2 127.486 -81.1911 64.235 0.075 2011 -2013 2 127.732 -84.0269 65.2897 0.075 2012 -2014 2 127.529 -87.0695 65.688 0.075 2013 -2015 2 127.55 -89.7111 66.967 0.075 2014 -2016 2 127.589 -91.9962 69.026 0.075 2015 -2017 2 127.652 -94.3092 71.0383 0.075 2016 -2018 2 128.121 -97.0815 72.2858 0.075 2017 -2019 2 128.7 -99.99 73 0.075 2018 -2020 2 131.11 -123.81 15.9333 0.075 1961 -2021 2 132.189 -125.316 12.8626 0.075 2020 -2022 2 133.298 -128.062 10.8598 0.075 2021 -2023 2 134.159 -130.819 8.74254 0.075 2022 -2024 2 136.011 -132.441 6.30638 0.075 2023 -2025 2 136.178 -135.139 4.30202 0.075 2024 -2026 2 135.53 -137.86 2.06667 0.075 2025 -2027 2 133.963 -122.675 15.6687 0.075 2020 -2028 2 136.929 -121.849 15.5195 0.075 2027 -2029 2 139.181 -119.772 15.1146 0.075 2028 -2030 2 140.898 -117.262 14.4545 0.075 2029 -2031 2 143.263 -115.369 14.6026 0.075 2030 -2032 2 145.873 -114.112 13.8982 0.075 2031 -2033 2 147.681 -112.843 11.9165 0.075 2032 -2034 2 148.147 -110.303 10.3088 0.075 2033 -2035 2 150.293 -108.226 9.92323 0.075 2034 -2036 2 152.937 -106.642 9.7047 0.075 2035 -2037 2 155.471 -104.856 9.42381 0.075 2036 -2038 2 157.747 -103.117 8.43085 0.075 2037 -2039 2 159.557 -101.791 6.30821 0.075 2038 -2040 2 161.604 -100.535 4.34113 0.075 2039 -2041 2 164.324 -99.0559 4.08618 0.075 2040 -2042 2 167.135 -98.3239 5.11324 0.075 2041 -2043 2 170.185 -98.3299 5.55466 0.075 2042 -2044 2 173.274 -98.3593 5.39828 0.075 2043 -2045 2 176.239 -97.5016 5.0985 0.075 2044 -2046 2 179.298 -96.9541 4.85044 0.075 2045 -2047 2 182.379 -96.5254 4.75357 0.075 2046 -2048 2 185.24 -96.1714 3.99922 0.075 2047 -2049 2 188.005 -95.515 2.78605 0.075 2048 -2050 2 190.563 -94.0855 1.94545 0.075 2049 -2051 2 193.02 -92.22 1.49333 0.075 2050 -2052 2 192.804 -91.1606 -1.58252 0.075 2051 -2053 2 191.982 -90.2555 -4.55321 0.075 2052 -2054 2 190.668 -89.3578 -7.39186 0.075 2053 -2055 2 189.435 -88.306 -10.218 0.075 2054 -2056 2 188.201 -87.3545 -13.0658 0.075 2055 -2057 2 186.63 -86.368 -15.7279 0.075 2056 -2058 2 185.454 -84.579 -18.0087 0.075 2057 -2059 2 185.6 -81.65 -19.2667 0.075 2058 -2060 2 187.936 -79.5351 -20.8382 0.075 2059 -2061 2 188.84 -77.6961 -23.589 0.075 2060 -2062 2 188.426 -76.0965 -26.7258 0.075 2061 -2063 2 187.813 -73.9982 -29.5181 0.075 2062 -2064 2 188.31 -71.89 -32.24 0.075 2063 -2065 2 184.61 -80.862 -22.158 0.075 2059 -2066 2 183.725 -79.5729 -24.8163 0.075 2065 -2067 2 183.063 -77.2506 -26.7824 0.075 2066 -2068 2 181.924 -74.3756 -27.2847 0.075 2067 -2069 2 181.054 -71.3922 -27.6869 0.075 2068 -2070 2 181.129 -68.3011 -28.1755 0.075 2069 -2071 2 181.995 -65.3113 -28.2311 0.075 2070 -2072 2 182.436 -62.345 -27.7367 0.075 2071 -2073 2 182.523 -59.2241 -27.5468 0.075 2072 -2074 2 182.184 -56.1745 -26.9654 0.075 2073 -2075 2 181.357 -53.2646 -26.8889 0.075 2074 -2076 2 182.032 -50.2086 -26.7903 0.075 2075 -2077 2 182 -47.2131 -26.4792 0.075 2076 -2078 2 180.961 -44.4032 -26.8239 0.075 2077 -2079 2 180.849 -41.2889 -27.3102 0.075 2078 -2080 2 180.818 -38.1583 -27.6468 0.075 2079 -2081 2 180.611 -35.0257 -27.4302 0.075 2080 -2082 2 180.002 -33.5417 -29.556 0.075 2081 -2083 2 179.38 -32.53 -32.48 0.075 2082 -2084 2 197.64 -91.55 4.72 0.075 2051 -2085 2 201.387 -92.5143 2.6674 0.075 2084 -2086 2 205.95 -92.15 2.33333 0.075 2085 -2087 2 201.209 -90.1662 4.71335 0.075 2084 -2088 2 204.913 -90.2038 5.37118 0.075 2087 -2089 2 208.148 -88.7244 6.80994 0.075 2088 -2090 2 211.734 -87.7826 6.20657 0.075 2089 -2091 2 214.61 -86.14 4.37333 0.075 2090 -2092 2 215.564 -84.3174 3.31335 0.075 2091 -2093 2 216.846 -83.3338 6.84553 0.075 2092 -2094 2 218.37 -81.9464 9.74047 0.075 2093 -2095 2 219.57 -78.3351 9.71115 0.075 2094 -2096 2 219.52 -74.48 9.96 0.075 2095 -2097 2 219.61 -71.118 10.4655 0.075 2096 -2098 2 220.58 -67.8942 10.003 0.075 2097 -2099 2 220.877 -64.5276 9.81841 0.075 2098 -2100 2 221.167 -61.369 10.78 0.075 2099 -2101 2 221.83 -58.42 12.3333 0.075 2100 -2102 2 220.232 -73.7108 13.3044 0.075 2096 -2103 2 220.944 -72.9416 16.6488 0.075 2102 -2104 2 221.64 -71.7138 14.0669 0.075 2103 -2105 2 222.2 -70.39 10.88 0.075 2104 -2106 2 216.772 -87.1516 2.05531 0.075 2091 -2107 2 217.803 -87.1479 -1.45776 0.075 2106 -2108 2 218.687 -88.1719 -4.63867 0.075 2107 -2109 2 219.006 -87.3473 -8.27179 0.075 2108 -2110 2 221.05 -87.72 -10.92 0.075 2109 -2111 2 223.761 -88.7587 -11.0599 0.075 2110 -2112 2 226.155 -90.1878 -9.66104 0.075 2111 -2113 2 227.97 -92.4106 -8.44188 0.075 2112 -2114 2 229.75 -93.4983 -6.31203 0.075 2113 -2115 2 231.484 -93.5719 -3.70139 0.075 2114 -2116 2 233.779 -93.8474 -1.70923 0.075 2115 -2117 2 236.444 -95.3585 -1.48474 0.075 2116 -2118 2 238.941 -97.1058 -0.91967 0.075 2117 -2119 2 241.415 -98.5315 0.375329 0.075 2118 -2120 2 243.72 -100.234 1.62172 0.075 2119 -2121 2 245.426 -102.748 2.361 0.075 2120 -2122 2 246.718 -105.193 3.59536 0.075 2121 -2123 2 248.014 -106.753 5.95067 0.075 2122 -2124 2 250.274 -108.263 7.23026 0.075 2123 -2125 2 252.633 -109.691 8.26709 0.075 2124 -2126 2 254.225 -110.803 10.7294 0.075 2125 -2127 2 255.844 -112.263 12.8781 0.075 2126 -2128 2 257.459 -114.747 13.8109 0.075 2127 -2129 2 258.809 -117.466 14.4659 0.075 2128 -2130 2 260.3 -119.88 15.7867 0.075 2129 -2131 2 220.555 -86.984 -15.0192 0.075 2110 -2132 2 221.286 -86.8996 -18.8596 0.075 2131 -2133 2 221.39 -86.37 -22.2533 0.075 2132 -2134 2 221.088 -89.4791 -21.9295 0.075 2133 -2135 2 221.797 -92.66 -21.5748 0.075 2134 -2136 2 223.17 -95.5999 -21.2308 0.075 2135 -2137 2 225.417 -97.938 -20.8335 0.075 2136 -2138 2 227.613 -100.044 -19.8016 0.075 2137 -2139 2 228.311 -102.379 -17.651 0.075 2138 -2140 2 228.528 -105.11 -15.8612 0.075 2139 -2141 2 228.827 -107.513 -13.6733 0.075 2140 -2142 2 228.574 -109.489 -11.1753 0.075 2141 -2143 2 226.25 -111.56 -10.36 0.075 2142 -2144 2 221.29 -83.4699 -23.6764 0.075 2133 -2145 2 222.01 -82.5227 -26.6391 0.075 2144 -2146 2 223.957 -81.6281 -27.8811 0.075 2145 -2147 2 226.275 -80.1232 -28.6723 0.075 2146 -2148 2 227.994 -80.2582 -31.4774 0.075 2147 -2149 2 229.082 -79.6244 -34.4808 0.075 2148 -2150 2 230.179 -77.5819 -36.7807 0.075 2149 -2151 2 231.514 -74.7611 -37.9026 0.075 2150 -2152 2 233.482 -72.3231 -38.9688 0.075 2151 -2153 2 235.55 -70.82 -40.96 0.075 2152 -2154 2 36.1649 -124.438 23.3514 0.075 1341 -2155 2 34.7443 -122.675 21.2092 0.075 2154 -2156 2 33.156 -120.839 19.2382 0.075 2155 -2157 2 31.2058 -118.812 17.9709 0.075 2156 -2158 2 28.7616 -117.219 16.8395 0.075 2157 -2159 2 26.6907 -115.377 15.4167 0.075 2158 -2160 2 24.5046 -114.423 13.5449 0.075 2159 -2161 2 21.8935 -112.765 13.1467 0.075 2160 -2162 2 19.0228 -111.737 13.7936 0.075 2161 -2163 2 16.0457 -110.988 14.4016 0.075 2162 -2164 2 12.9838 -110.364 14.521 0.075 2163 -2165 2 10.0238 -109.809 13.9932 0.075 2164 -2166 2 7.28328 -109.297 12.5688 0.075 2165 -2167 2 4.67947 -108.448 11.1089 0.075 2166 -2168 2 2.48316 -106.973 9.43518 0.075 2167 -2169 2 0.0744903 -105.404 8.3047 0.075 2168 -2170 2 -2.59 -103.78 8.24 0.075 2169 -2171 2 -2.34165 -103.434 4.45818 0.075 2170 -2172 2 -2.95986 -102.203 1.21289 0.075 2171 -2173 2 -5.25491 -101.062 -1.47791 0.075 2172 -2174 2 -7.67521 -100.212 -4.26753 0.075 2173 -2175 2 -9.55 -99.59 -7.49333 0.075 2174 -2176 2 -4.84029 -103.056 9.91286 0.075 2170 -2177 2 -6.30448 -103.139 13.351 0.075 2176 -2178 2 -7.6826 -104.446 16.4405 0.075 2177 -2179 2 -8.47 -104.85 20.0667 0.075 2178 -2180 2 41.23 -118.75 29.9867 0.075 1340 -2181 2 43.911 -120.357 30.287 0.075 2180 -2182 2 46.6287 -120.197 31.8035 0.075 2181 -2183 2 47.914 -118.306 34.1151 0.075 2182 -2184 2 49.2878 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a/examples/example_models/fs/str-fs-model1/morphology/morphology_hash_filename.json b/examples/example_models/fs/str-fs-model1/morphology/morphology_hash_filename.json deleted file mode 100644 index ad8a7c6f..00000000 --- a/examples/example_models/fs/str-fs-model1/morphology/morphology_hash_filename.json +++ /dev/null @@ -1,11 +0,0 @@ -{ - "m27aed108": "DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var6.swc", - "m2a6ab0d6": "DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var8.swc", - "m48f576bb": "DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var4.swc", - "m868d56db": "DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var3.swc", - "m9d1605c7": "DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var7.swc", - "ma88f99b9": "DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var0.swc", - "macdedfff": "DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var2.swc", - "meffc0dfa": "DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var1.swc", - "mf0a83676": "DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var5.swc" -} \ No newline at end of file diff --git a/examples/example_models/fs/str-fs-model1/parameters.json b/examples/example_models/fs/str-fs-model1/parameters.json deleted file mode 100644 index 0ac515e7..00000000 --- a/examples/example_models/fs/str-fs-model1/parameters.json +++ /dev/null @@ -1,570 +0,0 @@ -{ - "p31916918": [ - { - "param_name": "celsius", - "type": "global", - "value": 35 - }, - { - "param_name": "v_init", - "type": "global", - "value": -85 - }, - { - "dist_type": "uniform", - "param_name": "g_pas", - "sectionlist": "all", - "type": "section", - "value": 7.231405795469817e-05 - }, - { - "dist_type": "uniform", - "param_name": "e_pas", - "sectionlist": "all", - "type": "section", - "value": -68.76792868909992 - }, - { - "dist_type": "uniform", - "param_name": "cm", - "sectionlist": "all", - "type": "section", - "value": 1 - }, - { - "dist_type": "uniform", - "param_name": "Ra", - "sectionlist": "all", - "type": "section", - "value": 258.6848228255836 - }, - { - "dist_type": "uniform", - "param_name": "ek", - 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1.39 + ], + "steady_state_voltage_stimend": [ + -97.9656, + 1.0 + ], + "voltage_base": [ + -86.7085, + 1.0 + ], + "voltage_deflection": [ + -11.1805, + 1.0 + ] + } + }, + "IV_483": { + "soma": { + "decay_time_constant_after_stim": [ + 3.6119, + 1.39 + ], + "steady_state_voltage_stimend": [ + -91.9468, + 1.0 + ], + "voltage_base": [ + -85.7522, + 1.0 + ], + "voltage_deflection": [ + -5.9175, + 1.0 + ] + } + }, + "IV_489": { + "soma": { + "decay_time_constant_after_stim": [ + 5.4158, + 3.83 + ], + "steady_state_voltage_stimend": [ + -76.3287, + 1.0 + ], + "voltage_base": [ + -86.1513, + 1.0 + ], + "voltage_deflection": [ + 9.562, + 1.0 + ] + } + }, + "IDthresh-sub_518": { + "soma": { + "Spikecount": [ + 0.0, + 0.001 + ], + "decay_time_constant_after_stim": [ + 8.7684, + 3.83 + ], + "steady_state_voltage": [ + -87.7645, + 2.0 + ], + "steady_state_voltage_stimend": [ + -69.6508, + 1.0 + ], + "voltage_base": [ + -88.7376, + 1.0 + ], + "voltage_deflection": [ + 19.6401, + 1.0 + ], + "voltage_deflection_begin": [ + 16.8235, + 1.0 + ] + } + }, + "IDthresh_520": { + "soma": { + "AHP_depth_abs": [ + -65.5356, + 0.3159 + ], + "AHP_depth_abs_slow": [ + -65.7289, + 0.3032 + ], + "AP_begin_voltage": [ + -42.4681, + 1.0645 + ], + "inv_first_ISI": [ + 6.079, + 0.6079 + ], + "steady_state_voltage": [ + -85.8695, + 2.0 + ], + "time_to_first_spike": [ + 725.5, + 72.55 + ], + "voltage_base": [ + -87.2462, + 1.0 + ] + } + }, + "IDthresh_524": { + "soma": { + "AHP_depth_abs": [ + -64.7066, + 0.3157 + ], + "AHP_depth_abs_slow": [ + -64.31, + 0.2814 + ], + "AP_begin_voltage": [ + -41.7836, + 0.8857 + ], + "inv_first_ISI": [ + 17.2414, + 1.7241 + ], + "steady_state_voltage": [ + -85.678, + 2.0 + ], + "time_to_first_spike": [ + 175.9, + 17.59 + ], + "voltage_base": [ + -86.9676, + 1.0 + ] + } + }, + "IDthresh_530": { + "soma": { + "AHP_depth_abs": [ + -64.4305, + 0.3659 + ], + "AHP_depth_abs_slow": [ + -63.4691, + 0.3462 + ], + "AP_begin_voltage": [ + -41.9305, + 0.9892 + ], + "inv_first_ISI": [ + 29.8507, + 2.9851 + ], + "steady_state_voltage": [ + -86.705, + 2.0 + ], + "time_to_first_spike": [ + 136.4, + 13.64 + ], + "voltage_base": [ + -87.8294, + 1.0 + ] + } + }, + "IDthresh_538": { + "soma": { + "AHP_depth_abs": [ + -63.7869, + 2.5453 + ], + "AHP_depth_abs_slow": [ + -61.837, + 0.3704 + ], + "inv_first_ISI": [ + 42.5532, + 4.2553 + ], + "steady_state_voltage": [ + -87.2973, + 2.0 + ], + "time_to_first_spike": [ + 103.4, + 10.34 + ], + "voltage_base": [ + -87.9875, + 1.0 + ] + } + } +} diff --git a/examples/example_models/fs/model2/mechanisms.json b/examples/example_models/model2/config/mechanisms.json similarity index 99% rename from examples/example_models/fs/model2/mechanisms.json rename to examples/example_models/model2/config/mechanisms.json index 33034601..f3bde8f6 100644 --- a/examples/example_models/fs/model2/mechanisms.json +++ b/examples/example_models/model2/config/mechanisms.json @@ -15,4 +15,4 @@ "basal": [ "kir_fs" ] -} \ No newline at end of file +} diff --git a/examples/example_models/model2/config/parameters.json b/examples/example_models/model2/config/parameters.json new file mode 100644 index 00000000..de7b3972 --- /dev/null +++ b/examples/example_models/model2/config/parameters.json @@ -0,0 +1,142 @@ +[ + { + "param_name": "celsius", + "type": "global", + "value": 35 + }, + { + "param_name": "v_init", + "type": "global", + "value": -85 + }, + { + "param_name": "g_pas", + "sectionlist": "all", + "type": "section", + "dist_type": "uniform", + "bounds": [1e-5, 1e-3] + }, + { + "param_name": "e_pas", + "sectionlist": "all", + "type": "section", + "dist_type": "uniform", + "bounds": [-80, -60] + }, + { + "param_name": "cm", + "sectionlist": "all", + "type": "section", + "dist_type": "uniform", + "value": 1 + }, + { + "param_name": "Ra", + "sectionlist": "all", + "type": "section", + "dist_type": "uniform", + "bounds": [100, 300] + }, + { + "param_name": "ek", + "sectionlist": "somatic", + "type": "section", + "dist_type": "uniform", + "value": -105.9 + }, + { + "param_name": "ek", + "sectionlist": "basal", + "type": "section", + "dist_type": "uniform", + "value": -105.9 + }, + { + "param_name": "ena", + "sectionlist": "somatic", + "type": "section", + "dist_type": "uniform", + "value": 53.34 + }, + { + "param_name": "gbar_naf_fs", + "mech": "naf_fs", + "bounds": [0, 15] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "gbar_kdr_fs", + "mech": "kdr_fs", + "bounds": [0, 0.1] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "q_kdr_fs", + "mech": "kdr_fs", + "bounds": [3, 6] , + "dist_type": "uniform", + "mech_param": "q", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "gbar_kaf_fs", + "mech": "kaf_fs", + "bounds": [0, 1] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "gbar_kas_fs", + "mech": "kas_fs", + "bounds": [0, 0.01] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "pbar_caq_fs", + "mech": "caq_fs", + "value": 6e-6 , + "dist_type": "uniform", + "mech_param": "pbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "gbar_bk_fs", + "mech": "bk_fs", + "bounds": [0, 0.0001] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "gbar_kir_fs", + "mech": "kir_fs", + "bounds": [0, 0.01] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "gbar_kir_fs", + "mech": "kir_fs", + "bounds": [0, 0.001] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "basal" + } +] diff --git a/examples/example_models/model2/config/protocols.json b/examples/example_models/model2/config/protocols.json new file mode 100644 index 00000000..f3dd1882 --- /dev/null +++ b/examples/example_models/model2/config/protocols.json @@ -0,0 +1,130 @@ +{ + "IV_479": { + "stimuli": [ + { + "delay": 100.0, + "amp": -0.2491, + "duration": 1000.0, + "totduration": 1400.0 + }, + { + "delay": 0.0, + "amp": 0.1997, + "duration": 1400.0, + "totduration": 1400.0 + } + ] + }, + "IV_483": { + "stimuli": [ + { + "delay": 100.0, + "amp": -0.1069, + "duration": 1000.0, + "totduration": 1400.0 + }, + { + "delay": 0.0, + "amp": 0.1997, + "duration": 1400.0, + "totduration": 1400.0 + } + ] + }, + "IV_489": { + "stimuli": [ + { + "delay": 100.0, + "amp": 0.1063, + "duration": 1000.0, + "totduration": 1400.0 + }, + { + "delay": 0.0, + "amp": 0.1997, + "duration": 1400.0, + "totduration": 1400.0 + } + ] + }, + "IDthresh-sub_518": { + "stimuli": [ + { + "delay": 700.0, + "amp": 0.1602, + "duration": 2000.0, + "totduration": 3000.0 + }, + { + "delay": 0.0, + "amp": 0.1997, + "duration": 3000.0, + "totduration": 3000.0 + } + ] + }, + "IDthresh_520": { + "stimuli": [ + { + "delay": 700.0, + "amp": 0.1705, + "duration": 2000.0, + "totduration": 3000.0 + }, + { + "delay": 0.0, + "amp": 0.1998, + "duration": 3000.0, + "totduration": 3000.0 + } + ] + }, + "IDthresh_524": { + "stimuli": [ + { + "delay": 700.0, + "amp": 0.1916, + "duration": 2000.0, + "totduration": 3000.0 + }, + { + "delay": 0.0, + "amp": 0.1997, + "duration": 3000.0, + "totduration": 3000.0 + } + ] + }, + "IDthresh_530": { + "stimuli": [ + { + "delay": 700.0, + "amp": 0.2239, + "duration": 2000.0, + "totduration": 3000.0 + }, + { + "delay": 0.0, + "amp": 0.1997, + "duration": 3000.0, + "totduration": 3000.0 + } + ] + }, + "IDthresh_538": { + "stimuli": [ + { + "delay": 700.0, + "amp": 0.2666, + "duration": 2000.0, + "totduration": 3000.0 + }, + { + "delay": 0.0, + "amp": 0.1997, + "duration": 3000.0, + "totduration": 3000.0 + } + ] + } +} diff --git a/examples/example_models/model2/hall_of_fame.json b/examples/example_models/model2/hall_of_fame.json new file mode 100644 index 00000000..23f88aa6 --- /dev/null +++ b/examples/example_models/model2/hall_of_fame.json @@ -0,0 +1,54 @@ +[ + { + "Ra.all": 276.5200234075511, + "e_pas.all": -73.52220746575605, + "g_pas.all": 6.973968171121733e-05, + "gbar_bk_fs.somatic": 6.71436733678553e-06, + "gbar_kaf_fs.somatic": 0.6217414304714759, + "gbar_kas_fs.somatic": 0.008329304216030932, + "gbar_kdr_fs.somatic": 0.036044796239901436, + "gbar_kir_fs.basal": 0.00010737145174633665, + "gbar_kir_fs.somatic": 0.00316064963963324, + "gbar_naf_fs.somatic": 14.786795352780555, + "q_kdr_fs.somatic": 3.9563721375379024 + }, + { + "Ra.all": 253.45058033544774, + "e_pas.all": -68.76792868909992, + "g_pas.all": 7.226593452302076e-05, + "gbar_bk_fs.somatic": 9.863461421900856e-09, + "gbar_kaf_fs.somatic": 0.5638205989252036, + "gbar_kas_fs.somatic": 0.00999735462945325, + "gbar_kdr_fs.somatic": 0.04168686646249981, + "gbar_kir_fs.basal": 9.137607306847196e-05, + "gbar_kir_fs.somatic": 0.0050570038864330745, + "gbar_naf_fs.somatic": 14.726393532365059, + "q_kdr_fs.somatic": 3.317878661163175 + }, + { + "Ra.all": 258.6848228255836, + "e_pas.all": -68.76792868909992, + "g_pas.all": 7.231405795469817e-05, + "gbar_bk_fs.somatic": 5.6430495889155036e-05, + "gbar_kaf_fs.somatic": 0.5628915741084268, + "gbar_kas_fs.somatic": 0.00999735462945325, + "gbar_kdr_fs.somatic": 0.03996848856957873, + "gbar_kir_fs.basal": 9.137607306847196e-05, + "gbar_kir_fs.somatic": 0.0049996482294515825, + "gbar_naf_fs.somatic": 14.633431663402494, + "q_kdr_fs.somatic": 3.450013298929291 + }, + { + "Ra.all": 271.1423332094484, + "e_pas.all": -73.50988369698466, + "g_pas.all": 5.7053543105088026e-05, + "gbar_bk_fs.somatic": 2.408543584892474e-07, + "gbar_kaf_fs.somatic": 0.6228534738990917, + "gbar_kas_fs.somatic": 0.008935616761213844, + "gbar_kdr_fs.somatic": 0.039386685233452894, + "gbar_kir_fs.basal": 0.00010737145174633665, + "gbar_kir_fs.somatic": 0.00335261993870535, + "gbar_naf_fs.somatic": 14.529747797256295, + "q_kdr_fs.somatic": 3.4155541482191603 + } +] \ No newline at end of file diff --git a/examples/example_models/model2/mechanisms/bk_fs.mod b/examples/example_models/model2/mechanisms/bk_fs.mod new file mode 100644 index 00000000..21bef2fd --- /dev/null +++ b/examples/example_models/model2/mechanisms/bk_fs.mod @@ -0,0 +1,99 @@ +TITLE BK-type calcium activated K channel (KCa1.1) + +UNITS { + (molar) = (1/liter) + (mV) = (millivolt) + (mA) = (milliamp) + (mM) = (millimolar) + FARADAY = (faraday) (kilocoulombs) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX bk_fs + USEION ca READ cai + USEION k READ ek WRITE ik + RANGE gbar, ik +} + +PARAMETER { + gbar = 0.0 (mho/cm2) + k1 = 0.003 (mM) + k4 = 0.009 (mM) + d1 = 0.84 + d4 = 1.0 + q = 1 : body temperature 35 C +} + +ASSIGNED { + v (mV) + ik (mA/cm2) + celsius (degC) + cai (mM) + ek (mV) + oinf + otau (ms) +} + +STATE { o } + +BREAKPOINT { + SOLVE state METHOD cnexp + ik = gbar*o*(v-ek) +} + +DERIVATIVE state { + rate(v, cai) + o' = (oinf-o)/otau*q +} + +INITIAL { + rate(v, cai) + o = oinf +} + +PROCEDURE rate(v (mV), ca (mM)) { + LOCAL a, b, sum, z + UNITSOFF + z = 1e-3*2*FARADAY/(R*(celsius+273.15)) + a = 0.48*ca/(ca+k1*exp(-z*d1*v)) + b = 0.28/(1+ca/(k4*exp(-z*d4*v))) + sum = a+b + oinf = a/sum + otau = 1/sum + UNITSON +} + +COMMENT + +Experimental data was obtained from BKCa channels from rat brain injected +as cRNAs into Xenopus oocytes [1]. Electrophysiological recordings were +performed at room temperature 22-24 C [1, supporting online material]. + +Original model [2, model 3 in Tab.1] was implemented by De Schutter +and adapted by Kai Du. In the model revisions [3,4] parameters k1 and k4 +[2, channel A in Tab.2] were adjusted to fit rat/Xenopus data +[1, Fig.3C and Fig.4A, 10 uM Ca] at body temperature 35 C. + +NEURON implementation by Alexander Kozlov . + +[1] Berkefeld H, Sailer CA, Bildl W, Rohde V, Thumfart JO, Eble S, +Klugbauer N, Reisinger E, Bischofberger J, Oliver D, Knaus HG, Schulte U, +Fakler B (2006) BKCa-Cav channel complexes mediate rapid and localized +Ca2+-activated K+ signaling. Science 314(5799):615-20. + +[2] Moczydlowski E, Latorre R (1983) Gating kinetics of Ca2+-activated K+ +channels from rat muscle incorporated into planar lipid bilayers. Evidence +for two voltage-dependent Ca2+ binding reactions. J Gen Physiol +82(4):511-42. + +[3] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH, +Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on +calcium influx and spike timing-dependent plasticity in striatal medium +spiny neurons. PLoS Comput Biol 8(4):e1002493. + +[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +ENDCOMMENT diff --git a/examples/example_models/model2/mechanisms/cadyn_fs.mod b/examples/example_models/model2/mechanisms/cadyn_fs.mod new file mode 100644 index 00000000..17651d8a --- /dev/null +++ b/examples/example_models/model2/mechanisms/cadyn_fs.mod @@ -0,0 +1,59 @@ +TITLE Calcium dynamics for N, P/Q, R calcium pool + +NEURON { + SUFFIX cadyn_fs + USEION ca READ ica, cai WRITE cai VALENCE 2 + RANGE pump, cainf, taur, drive +} + +UNITS { + (molar) = (1/liter) + (mM) = (millimolar) + (um) = (micron) + (mA) = (milliamp) + (msM) = (ms mM) + FARADAY = (faraday) (coulomb) +} + +PARAMETER { + drive = 10000 (1) + depth = 0.1 (um) + cainf = 10e-6 (mM) + taur = 43 (ms) + kt = 1e-4 (mM/ms) + kd = 1e-4 (mM) + pump = 0.02 +} + +STATE { cai (mM) } + +INITIAL { cai = cainf } + +ASSIGNED { + ica (mA/cm2) + drive_channel (mM/ms) + drive_pump (mM/ms) +} + +BREAKPOINT { + SOLVE state METHOD cnexp +} + +DERIVATIVE state { + drive_channel = -drive*ica/(2*FARADAY*depth) + if (drive_channel <= 0.) { drive_channel = 0. } + drive_pump = -kt*cai/(cai+kd) + cai' = (drive_channel+pump*drive_pump+(cainf-cai)/taur) +} + +COMMENT + +Original NEURON model by Wolf (2005) and Destexhe (1992). Adaptation by +Alexander Kozlov . + +[1] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +ENDCOMMENT diff --git a/examples/example_models/model2/mechanisms/can_fs.mod b/examples/example_models/model2/mechanisms/can_fs.mod new file mode 100644 index 00000000..0bea8c12 --- /dev/null +++ b/examples/example_models/model2/mechanisms/can_fs.mod @@ -0,0 +1,121 @@ +TITLE N-type calcium current (Cav2.2) + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (S) = (siemens) + (molar) = (1/liter) + (mM) = (millimolar) + FARADAY = (faraday) (coulomb) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX can_fs + USEION ca READ cai, cao WRITE ica VALENCE 2 + RANGE pbar, ica +} + +PARAMETER { + pbar = 0.0 (cm/s) + a = 0.21 + :q = 1 : room temperature 22-25 C + q = 3 : body temperature 35 C +} + +ASSIGNED { + v (mV) + ica (mA/cm2) + eca (mV) + celsius (degC) + cai (mM) + cao (mM) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + ica = pbar*m*m*(h*a+1-a)*ghk(v, cai, cao) +} + +INITIAL { + rates() + m = minf + h = hinf +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-3))/(-8))) + mtau = 0.06+1/(exp((v-25)/18)+exp((v-(-31))/(-44))) + hinf = 1/(1+exp((v-(-74.8))/6.5)) + htau = 70 + UNITSON +} + +FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) { + LOCAL z, eci, eco + z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15)) + eco = co*efun(z) + eci = ci*efun(-z) + ghk = (1e-3)*2*FARADAY*(eci-eco) +} + +FUNCTION efun(z) { + if (fabs(z) < 1e-4) { + efun = 1-z/2 + }else{ + efun = z/(exp(z)-1) + } +} + +COMMENT + +Model is based on mixed data. Activation curve is from neostriatal +medium spiny neurons of adult P28+ rats [1, Fig.12F], unspecified +recording temperature. Potentials were not corrected for the liquid +junction potential, which was estimated to be 7 mV. Activation time +constant is from the rodent neuron culture (both rat and mouse cells), +room temperature 22-25 C [2, Fig.15B]. Inactivation data is from human +(HEK) cells [3, Tab.1, Tab.2], supposedly at room temperature. + +Kinetics of m2h type is used [2, Fig.5]. Activation of m^2 is fitted +to the experimental data [1,4], activation time constant corresponds +to m^2 already [2]. Original model [5,4] was modified by Alexander +Kozlov . Activation time constant was refitted to avoid +singularity in the expression. Temperature correction factor 3 is used +for body temperature [4,5]. + +[1] Bargas J, Howe A, Eberwine J, Cao Y, Surmeier DJ (1994) Cellular +and molecular characterization of Ca2+ currents in acutely isolated, +adult rat neostriatal neurons. J Neurosci 14(11 Pt 1):6667-86. + +[2] Kasai H, Neher E (1992) Dihydropyridine-sensitive and +omega-conotoxin-sensitive calcium channels in a mammalian +neuroblastoma-glioma cell line. J Physiol 448:161-88. + +[3] McNaughton NC, Randall AD (1997) Electrophysiological properties of +the human N-type Ca2+ channel: I. Channel gating in Ca2+, Ba2+ and Sr2+ +containing solutions. Neuropharmacology 36(7):895-915. + +[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +[5] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +ENDCOMMENT diff --git a/examples/example_models/model2/mechanisms/caq_fs.mod b/examples/example_models/model2/mechanisms/caq_fs.mod new file mode 100644 index 00000000..7d4efcf9 --- /dev/null +++ b/examples/example_models/model2/mechanisms/caq_fs.mod @@ -0,0 +1,100 @@ +TITLE Q-type calcium current (Cav2.1) + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (S) = (siemens) + (molar) = (1/liter) + (mM) = (millimolar) + FARADAY = (faraday) (coulomb) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX caq_fs + USEION ca READ cai, cao WRITE ica VALENCE 2 + RANGE pbar, ica +} + +PARAMETER { + pbar = 0.0 (cm/s) + :q = 1 : room temperature 22 C + q = 3 : body temperature 35 C +} + +ASSIGNED { + v (mV) + ica (mA/cm2) + eca (mV) + celsius (degC) + cai (mM) + cao (mM) + minf + mtau (ms) +} + +STATE { m } + +BREAKPOINT { + SOLVE states METHOD cnexp + ica = pbar*m*m*ghk(v, cai, cao) +} + +INITIAL { + rates() + m = minf +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-16.3))/(-7.9))) + mtau = 1.13*2 + UNITSON +} + +FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) { + LOCAL z, eci, eco + z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15)) + if(z == 0) { + z = z+1e-6 + } + eco = co*(z)/(exp(z)-1) + eci = ci*(-z)/(exp(-z)-1) + ghk = (1e-3)*2*FARADAY*(eci-eco) +} + +COMMENT + +Activation curve was reconstructed for cultured NAc neurons from P5-P32 +Charles River rat pups [1]. Activation time constant was measured in +culture neurons from cerebellum of P2-P5 rat pups [2] at room temperature +22 C. + +Original NEURON model by Wolf (2005) [3] was modified by Alexander Kozlov +. Activation curve was fitted to m2 kinetics [4], +activation time constant was scaled up as well. + +[1] Churchill D, Macvicar BA (1998) Biophysical and pharmacological +characterization of voltage-dependent Ca2+ channels in neurons isolated +from rat nucleus accumbens. J Neurophysiol 79(2):635-47. + +[2] Randall A, Tsien RW (1995) Pharmacological dissection of multiple +types of Ca2+ channel currents in rat cerebellar granule neurons. J +Neurosci 15(4):2995-3012. + +[3] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[4] Brown AM, Schwindt PC, Crill WE (1993) Voltage dependence and +activation kinetics of pharmacologically defined components of the +high-threshold calcium current in rat neocortical neurons. J Neurophysiol +70(4):1530-43. + +ENDCOMMENT diff --git a/examples/example_models/model2/mechanisms/car_fs.mod b/examples/example_models/model2/mechanisms/car_fs.mod new file mode 100644 index 00000000..2bc97677 --- /dev/null +++ b/examples/example_models/model2/mechanisms/car_fs.mod @@ -0,0 +1,113 @@ +TITLE R-type calcium current (Cav2.3) + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (S) = (siemens) + (molar) = (1/liter) + (mM) = (millimolar) + FARADAY = (faraday) (coulomb) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX car_fs + USEION ca READ cai, cao WRITE ica VALENCE 2 + RANGE pbar, ica +} + +PARAMETER { + pbar = 0.0 (cm/s) + :q = 1 : room temperature 22 C + q = 3 : body temperature 35 C +} + +ASSIGNED { + v (mV) + ica (mA/cm2) + eca (mV) + celsius (degC) + cai (mM) + cao (mM) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + ica = pbar*m*m*m*h*ghk(v, cai, cao) +} + +INITIAL { + rates() + m = minf + h = hinf +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-29))/(-9.6))) + mtau = 5.1 + hinf = 1/(1+exp((v-(-33.3))/17)) + htau = 22+80/(1+exp((v-(-19))/5)) + UNITSON +} + +FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) { + LOCAL z, eci, eco + z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15)) + eco = co*efun(z) + eci = ci*efun(-z) + ghk = (1e-3)*2*FARADAY*(eci-eco) +} + +FUNCTION efun(z) { + if (fabs(z) < 1e-4) { + efun = 1-z/2 + }else{ + efun = z/(exp(z)-1) + } +} + +COMMENT + +Original data by Foehring et al (2000) [1] for dissociated MSNs from +P28-P42 Sprague-Dawley rat brain. Unspecified recording temperature. The +liquid junction potential was around 8 mV and was not corrected. Kinetics +of m3h type was fitted. Inactivation time constants were measured in +neurons from endopiriform nucleus of P7-P21 Hartley guinea pigs [2] +at room temperature 22 C. + +Original NEURON model by Wolf (2005) [3] modified by Alexander Kozlov +. Activation curve fitted to m^3 kinetics as in [4], +activation time constant matched m^3 originally [1]. Smooth fit of +inactivation time constant from [2,3]. + +[1] Foehring RC, Mermelstein PG, Song WJ, Ulrich S, Surmeier DJ +(2000) Unique properties of R-type calcium currents in neocortical and +neostriatal neurons. J Neurophysiol 84(5):2225-36. + +[2] Brevi S, de Curtis M, Magistretti J (2001) Pharmacological and +biophysical characterization of voltage-gated calcium currents in the +endopiriform nucleus of the guinea pig. J Neurophysiol 85(5):2076-87. + +[3] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +ENDCOMMENT diff --git a/examples/example_models/model2/mechanisms/kaf_fs.mod b/examples/example_models/model2/mechanisms/kaf_fs.mod new file mode 100644 index 00000000..8f364c5c --- /dev/null +++ b/examples/example_models/model2/mechanisms/kaf_fs.mod @@ -0,0 +1,128 @@ +TITLE Fast A-type potassium current (Kv4.2) + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + +NEURON { + SUFFIX kaf_fs + USEION k READ ek WRITE ik + RANGE gbar, gk, ik, q + RANGE modDA, maxModDA, levelDA +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.0 (S/cm2) + :q = 1 : room temperature (unspecified) + q = 3 : body temperature 35 C + modDA = 0 + maxModDA = 1 + levelDA = 0 +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + gk (S/cm2) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + gk = gbar*m*m*h*modulationDA() + ik = gk*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +INITIAL { + rates() + m = minf + h = hinf +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-10))/(-17.7))) + mtau = (0.9+1.1/(1+exp((v-(-30))/10)))*2 + hinf = 1/(1+exp((v-(-75.6))/11.8)) + htau = 14 + UNITSON +} + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + +COMMENT + +Original data by Tkatch et al (2000) [1]. Neostriatal neurons were acutely +dissociated from young adult rats, age P28-P42. Electrophysiological +recordings were done at unspecified temperature (room temperature 20-22 C +assumed). Potentials were not corrected for the liquid junction potential +(estimated 1-2 mV). + +Conductance kinetics of m2h type is used [2]. Activation m^1 matches +experimental data [1, Fig.2C]. Activation time constants were fitted to +tabulated data [1, Fig.2B] by Alexander Kozlov and scaled +up x2 for m2 kinetics. Slope of inactivation function fitted to the data +[1, Fig.3B] with half inactivation potential -75.6 mV. Temperature factor +q between 3 [2] and 1.5 [3] was used for body temperature. + +[1] Tkatch T, Baranauskas G, Surmeier DJ (2000) Kv4.2 mRNA abundance and +A-type K(+) current amplitude are linearly related in basal ganglia and +basal forebrain neurons. J Neurosci 20(2):579-88. + +[2] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[3] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH, +Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on +calcium influx and spike timing-dependent plasticity in striatal medium +spiny neurons. PLoS Comput Biol 8(4):e1002493. + +ENDCOMMENT diff --git a/examples/example_models/model2/mechanisms/kas_fs.mod b/examples/example_models/model2/mechanisms/kas_fs.mod new file mode 100644 index 00000000..b08ddb6b --- /dev/null +++ b/examples/example_models/model2/mechanisms/kas_fs.mod @@ -0,0 +1,128 @@ +TITLE Slowly inactivating A-type potassium current (Kv1.2) + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + +NEURON { + SUFFIX kas_fs + USEION k READ ek WRITE ik + RANGE gbar, gk, ik, shift, q + RANGE modDA, maxModDA, levelDA +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.0 (S/cm2) + a = 0.8 + :q = 1 : room temperature 22-24 C + q = 3 : body temperature 33 C + shift = 0 + modDA = 0 + maxModDA = 1 + levelDA = 0 +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + gk (S/cm2) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + gk = gbar*m*m*(h*a+1-a)*modulationDA() + ik = gk*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +INITIAL { + rates() + m = minf + h = hinf +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-27)-shift)/(-16))) + mtau = 3.4+89.2*exp(-((v-(-34.3))/30.1)^2) + hinf = 1/(1+exp((v-(-33.5)-shift)/21.5)) + htau = 548.7*6/(exp((v-(-96))/(-29.01))+exp((v-(-96))/100)) + UNITSON +} + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + +COMMENT + +Experimental data by Shen et al (2004) [1]. Medium spiny neurons were +acutely dissociated from from young adult (P21-P28) Sprague-Dawley rat +brain. All recordings were conducted at 22-24 C. No correction for the +liquid junction potential was reported. + +Conductance kinetics of m2h type is used [1] with partial inactivation, +m2 (a h + (1-a)). Fraction a is set to 0.8, as in [1, Fig.6B]; other +values for a are possible [2] (see also kas.mod in companion code). +Equation for htau [1] is corrected to match the authors' data [1, Fig.6B] +by Alexander Kozlov . Time constants were corrected to +body temperature with factor q=3 [1,3]. + +[1] Shen W, Hernandez-Lopez S, Tkatch T, Held JE, Surmeier DJ (2004) +Kv1.2-containing K+ channels regulate subthreshold excitability of +striatal medium spiny neurons. J Neurophysiol 91(3):1337-49. + +[2] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[3] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +ENDCOMMENT diff --git a/examples/example_models/model2/mechanisms/kdr_fs.mod b/examples/example_models/model2/mechanisms/kdr_fs.mod new file mode 100644 index 00000000..99b8287d --- /dev/null +++ b/examples/example_models/model2/mechanisms/kdr_fs.mod @@ -0,0 +1,80 @@ +TITLE Fast delayed rectifier potassium current (Kv3.1/3.2) + +NEURON { + SUFFIX kdr_fs + USEION k READ ek WRITE ik + RANGE gbar, gk, ik, q +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.0 (S/cm2) + :q = 1 : room temperature + q = 3 : body temperature 35 C +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + gk (S/cm2) + minf + mtau (ms) +} + +STATE { m } + +BREAKPOINT { + SOLVE states METHOD cnexp + gk = gbar*m + ik = gk*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q +} + +INITIAL { + rates() + m = minf +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-13))/(-6))) + mtau = 11.1 + UNITSON +} + +COMMENT + +Original data and model by Baranauskas et al (1999) [1] for globus +pallidus neurons from young adult rat. The temperature was not +specified. Potentials were not corrected for the liquid junction +potential, which was estimated to be 1-2 mV. + +Kinetics of m1 type is used as in [2,3]. Room temperature 20-23 C +is assumed. + +NEURON implementation by Alexander Kozlov . + +[1] Baranauskas G, Tkatch T, Surmeier DJ (1999) Delayed rectifier currents +in rat globus pallidus neurons are attributable to Kv2.1 and Kv3.1/3.2 +K(+) channels. J Neurosci 19(15):6394-404. + +[2] Migliore M, Hoffman DA, Magee JC, Johnston D (1999) Role of an +A-type K+ conductance in the back-propagation of action potentials in the +dendrites of hippocampal pyramidal neurons. J Comput Neurosci 7(1):5-15. + +[3] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH, +Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on +calcium influx and spike timing-dependent plasticity in striatal medium +spiny neurons. PLoS Comput Biol 8(4):e1002493. + +ENDCOMMENT diff --git a/examples/example_models/model2/mechanisms/kir_fs.mod b/examples/example_models/model2/mechanisms/kir_fs.mod new file mode 100644 index 00000000..3bd8313b --- /dev/null +++ b/examples/example_models/model2/mechanisms/kir_fs.mod @@ -0,0 +1,130 @@ +TITLE Non-inactivating inwardly rectifying potassium current (Kir2.3) + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + +NEURON { + SUFFIX kir_fs + USEION k READ ek WRITE ik + RANGE gbar, gk, ik, shift + RANGE modDA, maxModDA, levelDA +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.0 (S/cm2) + shift = 0 + q = 1 : body temperature 35 C + modDA = 0 + maxModDA = 1 + levelDA = 0 +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + gk (S/cm2) + minf + mtau (ms) +} + +STATE { m } + +BREAKPOINT { + SOLVE states METHOD cnexp + gk = gbar*m*modulationDA() + ik = gk*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q +} + +INITIAL { + rates() + m = minf +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-82)-shift)/13)) + mtau = 1/(exp((v-(-103)-shift)/(-14.5))+0.125/(1+exp((v-(-35)-shift)/(-19)))) + UNITSON +} + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + + +COMMENT + +Original model by Wolf et al (2005) [1] for the rat MSN cells from the +nucleus accumbens. The activation curve was fitted to a mouse Kir2.1 +channel expressed in HEK cells [2] and shifted to match extracellular +concentration of K in rat. Time constants were derived from Aplysia data +[3] and adjusted to match the rat experiments [1]. Time constant was +further tuned [4] to fit the rat data below -80 mV [5]. Kinetics is +corrected to the body temperature 35 C. + +Non-inactivating Kir current was observed in cells expressing Kir2.2 +and/or Kir2.3 [5]. Activation variable with m^1 kinetics is used [1,4]. +Smooth fit of the time constants by Alexander Kozlov . + +[1] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[2] Kubo Y, Murata Y (2001) Control of rectification and permeation by +two distinct sites after the second transmembrane region in Kir2.1 K+ +channel. J Physiol 531, 645-660. + +[3] Hayashi H, Fishman HM (1988) Inward rectifier K+ channel kinetics +from analysis of the complex conductance of aplysia neuronal membrane. +Biophys J 53, 747-757. + +[4] Steephen JE, Manchanda R (2009) Differences in biophysical properties +of nucleus accumbens medium spiny neurons emerging from inactivation of +inward rectifying potassium currents. J Comput Neurosci 27(3):453-70 + +[5] Mermelstein PG, Song WJ, Tkatch T, Yan Z, Surmeier DJ (1998) +Inwardly rectifying potassium (IRK) currents are correlated with IRK +subunit expression in rat nucleus accumbens medium spiny neurons. J +Neurosci 18(17):6650-61. + +ENDCOMMENT diff --git a/examples/example_models/model2/mechanisms/naf_fs.mod b/examples/example_models/model2/mechanisms/naf_fs.mod new file mode 100644 index 00000000..3dcfc3a9 --- /dev/null +++ b/examples/example_models/model2/mechanisms/naf_fs.mod @@ -0,0 +1,119 @@ +TITLE Fast transient sodium current + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + +NEURON { + SUFFIX naf_fs + USEION na READ ena WRITE ina + RANGE gbar, gna, ina, q + RANGE modDA, maxModDA, levelDA +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.0 (S/cm2) + :q = 1 : room temperature 22 C + q = 1.8 : body temperature 35 C + modDA = 0 + maxModDA = 1 + levelDA = 0 +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + gna (S/cm2) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + gna = gbar*m*m*m*h*modulationDA() + ina = gna*(v-ena) +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +INITIAL { + rates() + m = minf + h = hinf +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-25))/(-10))) + mtau = 0.33+1/(exp((v-(-62))/14)+exp((v-(-60))/(-17))) + hinf = 1/(1+exp((v-(-62))/6)) + htau = 0.6+1/(exp((v-(-44))/8)+exp((v-(-99))/(-44))) + UNITSON +} + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + +COMMENT + +Original data by Ogata and Tatebayashi (1990) [1]. Neostriatal neurons +of medium size (putative medium spiny neurons) freshly isolated from +the adult guinea pig brain (either sex, 200 g). Data compensated for +the liquid junction potential (-13 mV). Experiments carried out at room +temperature (22 C). Conductance fitted by m3h kinetics. + +Smooth fit of mtau and htau data [1] by Alexander Kozlov +assuming natural logarithm of tau values [1, Figs. 5 and 9] and +temperature correction factor of 1.8-2.1 [2] as suggested by Robert +Lindroos . + +[1] Ogata N, Tatebayashi H (1990) Sodium current kinetics in freshly +isolated neostriatal neurones of the adult guinea pig. Pflugers Arch +416(5):594-603. + +[2] Schwarz JR (1986) The effect of temperature on Na currents in rat +myelinated nerve fibres. Pflugers Arch. 406(4):397-404. + +ENDCOMMENT diff --git a/examples/example_models/model2/mechanisms/sk_fs.mod b/examples/example_models/model2/mechanisms/sk_fs.mod new file mode 100644 index 00000000..4f2c3e34 --- /dev/null +++ b/examples/example_models/model2/mechanisms/sk_fs.mod @@ -0,0 +1,83 @@ +TITLE SK-type calcium activated K channel (KCa2.2) + +UNITS { + (molar) = (1/liter) + (mV) = (millivolt) + (mA) = (milliamp) + (mM) = (millimolar) +} + +NEURON { + SUFFIX sk_fs + USEION ca READ cai + USEION k READ ek WRITE ik + RANGE gbar, ik +} + +PARAMETER { + gbar = 0.0 (mho/cm2) + :q = 1 : room temperature + q = 3 : body temperature +} + +ASSIGNED { + v (mV) + ik (mA/cm2) + cai (mM) + ek (mV) + oinf + otau (ms) +} + +STATE { o } + +BREAKPOINT { + SOLVE state METHOD cnexp + ik = gbar*o*(v-ek) +} + +DERIVATIVE state { + rate(v, cai) + o' = (oinf-o)/otau*q +} + +INITIAL { + rate(v, cai) + o = oinf +} + +PROCEDURE rate(v (mV), ca (mM)) { + LOCAL a + a = (ca/0.57e-3)^5.2 + oinf = a/(1+a) + otau = 4.9 +} + +COMMENT + +Experimental data was obtained for the apamin-sensitive clone rSK2 from +rat brain cDNA expressed in Xenopus oocytes [1,2]. All experiments were +performed at room tempretaure. + +Original model [3] used calcium dependence from [2, Fig.2] and calcium +activation time constant from [1, Fig.13]. NEURON implementation by +Alexander Kozlov follows the revised model [4]. + +[1] Hirschberg B, Maylie J, Adelman JP, Marrion NV (1998) Gating of +recombinant small-conductance Ca-activated K+ channels by calcium. J +Gen Physiol 111(4):565-81. + +[2] Maylie J, Bond CT, Herson PS, Lee WS, Adelman JP (2004) Small +conductance Ca2+-activated K+ channels and calmodulin. J Physiol 554(Pt +2):255-61. + +[3] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH, +Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on +calcium influx and spike timing-dependent plasticity in striatal medium +spiny neurons. PLoS Comput Biol 8(4):e1002493. + +[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +ENDCOMMENT diff --git a/examples/example_models/fs/str-fs-model1/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var0.swc b/examples/example_models/model2/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var0.swc similarity index 100% rename from examples/example_models/fs/str-fs-model1/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var0.swc rename to examples/example_models/model2/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var0.swc diff --git a/examples/example_models/fs/str-fs-model1/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var1.swc b/examples/example_models/model2/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var1.swc similarity index 100% rename from examples/example_models/fs/str-fs-model1/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var1.swc rename to examples/example_models/model2/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var1.swc diff --git a/examples/example_models/fs/str-fs-model1/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var2.swc b/examples/example_models/model2/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var2.swc similarity index 100% rename from examples/example_models/fs/str-fs-model1/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var2.swc rename to examples/example_models/model2/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var2.swc diff --git a/examples/example_models/fs/str-fs-model1/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var3.swc b/examples/example_models/model2/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var3.swc similarity index 100% rename from examples/example_models/fs/str-fs-model1/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var3.swc rename to examples/example_models/model2/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var3.swc diff --git a/examples/example_models/fs/str-fs-model1/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var4.swc b/examples/example_models/model2/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var4.swc similarity index 100% rename from examples/example_models/fs/str-fs-model1/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var4.swc rename to examples/example_models/model2/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var4.swc diff --git a/examples/example_models/fs/str-fs-model1/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var5.swc b/examples/example_models/model2/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var5.swc similarity index 100% rename from examples/example_models/fs/str-fs-model1/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var5.swc rename to examples/example_models/model2/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var5.swc diff --git a/examples/example_models/fs/str-fs-model1/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var6.swc b/examples/example_models/model2/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var6.swc similarity index 100% rename from examples/example_models/fs/str-fs-model1/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var6.swc rename to examples/example_models/model2/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var6.swc diff --git a/examples/example_models/fs/str-fs-model1/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var7.swc b/examples/example_models/model2/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var7.swc similarity index 100% rename from examples/example_models/fs/str-fs-model1/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var7.swc rename to examples/example_models/model2/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var7.swc diff --git a/examples/example_models/fs/str-fs-model1/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var8.swc b/examples/example_models/model2/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var8.swc similarity index 100% rename from examples/example_models/fs/str-fs-model1/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var8.swc rename to examples/example_models/model2/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var8.swc diff --git a/examples/example_models/fs/str-fs-model1/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3.swc b/examples/example_models/model2/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3.swc similarity index 100% rename from examples/example_models/fs/str-fs-model1/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3.swc rename to examples/example_models/model2/morphology/DR-rat-Mar-13-08-1-536-R-cor-rep-res3.swc diff --git a/examples/example_models/model2/val_models.json b/examples/example_models/model2/val_models.json new file mode 100644 index 00000000..3905b305 --- /dev/null +++ b/examples/example_models/model2/val_models.json @@ -0,0 +1,15390 @@ +[ + { + "ephys": "161024_FS16", + "morph": "DR-rat-Mar-13-08-1-536-R-cor-rep-res3-var0.swc", + "par": 0, + "iv_curve": { + "stim": [ + -100.3, + -96.78241206030152, + -93.264824120603, + -89.74723618090452, + -86.22964824120605, + -82.71206030150752, + 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examples/example_models/str-dspn-model1/best_models.json diff --git a/examples/example_models/str-dspn-model1/config/features.json b/examples/example_models/str-dspn-model1/config/features.json new file mode 100644 index 00000000..e83800a3 --- /dev/null +++ b/examples/example_models/str-dspn-model1/config/features.json @@ -0,0 +1,242 @@ +{ + "IV_354": { + "soma": { + "decay_time_constant_after_stim": [ + 3.3069, + 3.688 + ], + "steady_state_voltage_stimend": [ + -99.5983, + 1.0 + ], + "voltage_base": [ + -87.76, + 1.0 + ] + } + }, + "IV_358": { + "soma": { + "decay_time_constant_after_stim": [ + 4.5541, + 3.688 + ], + "steady_state_voltage_stimend": [ + -94.2423, + 1.0 + ], + "voltage_base": [ + -87.7868, + 1.0 + ] + } + }, + "IDthresh-sub_397": { + "soma": { + "Spikecount": [ + 0, + 0.001 + ], + "decay_time_constant_after_stim": [ + 15.0132, + 3.222 + ], + "steady_state_voltage_stimend": [ + -60.125, + 1.0 + ], + "voltage_base": [ + -87.8737, + 1.0 + ], + "voltage_deflection_begin": [ 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0.7543, + 0.0571 + ], + "steady_state_voltage": [ + -87.1295, + 0.5 + ], + "time_to_first_spike": [ + 116.1, + 11.61 + ], + "voltage_base": [ + -88.4495, + 1.0 + ] + } + } +} diff --git a/examples/example_models/dspn/str-dspn-model1/mechanisms.json b/examples/example_models/str-dspn-model1/config/mechanisms.json similarity index 100% rename from examples/example_models/dspn/str-dspn-model1/mechanisms.json rename to examples/example_models/str-dspn-model1/config/mechanisms.json diff --git a/examples/example_models/example-option-2/config/parameters.json b/examples/example_models/str-dspn-model1/config/parameters.json similarity index 98% rename from examples/example_models/example-option-2/config/parameters.json rename to examples/example_models/str-dspn-model1/config/parameters.json index 22947835..09f9aa46 100644 --- a/examples/example_models/example-option-2/config/parameters.json +++ b/examples/example_models/str-dspn-model1/config/parameters.json @@ -214,7 +214,7 @@ "mech": "naf_ms", "bounds": [0, 0.1] , "dist": "((0.1) + (0.9)/(1 + exp(({distance}-(40))/(10))))*{value}", - "dist_type": "distance", + "dist_type": "uniform", "mech_param": "gbar", "type": "range", "sectionlist": "basal" @@ -224,7 +224,7 @@ "mech": "kaf_ms", "bounds": [0.01, 0.5] , "dist": "((1.0) + (0.5)/(1 + exp(({distance}-(120))/(-30))))*{value}", - "dist_type": "distance", + "dist_type": "uniform", "mech_param": "gbar", "type": "range", "sectionlist": "basal" @@ -243,7 +243,7 @@ "mech": "kas_ms", "bounds": [0, 0.01] , "dist": "((1.0) + (9.0)*exp(({distance}-(0.0))/(-5.0)))*{value}", - "dist_type": "distance", + "dist_type": "uniform", "mech_param": "gbar", "type": "range", "sectionlist": "basal" @@ -298,7 +298,7 @@ "mech": "cat32_ms", "value": 0.2e-8 , "dist": "((0.0) + (1.0)/(1 + exp(({distance}-(120))/(-30))))*{value}", - "dist_type": "distance", + "dist_type": "uniform", "mech_param": "pbar", "type": "range", "sectionlist": "basal" @@ -308,7 +308,7 @@ "mech": "cat33_ms", "value": 0.2e-8 , "dist": "((0.0) + (1.0)/(1 + exp(({distance}-(120))/(-30))))*{value}", - "dist_type": "distance", + "dist_type": "uniform", "mech_param": "pbar", "type": "range", "sectionlist": "basal" diff --git a/examples/example_models/str-dspn-model1/config/protocols.json b/examples/example_models/str-dspn-model1/config/protocols.json new file mode 100644 index 00000000..411d1883 --- /dev/null +++ b/examples/example_models/str-dspn-model1/config/protocols.json @@ -0,0 +1,98 @@ +{ + "IV_354": { + "stimuli": [ + { + "delay": 100.0, + "amp": -0.4425, + "duration": 1000.0, + "totduration": 1400.0 + }, + { + "delay": 0.0, + "amp": 0.1024, + "duration": 1400.0, + "totduration": 1400.0 + } + ] + }, + "IV_358": { + "stimuli": [ + { + "delay": 100.0, + "amp": -0.1892, + "duration": 1000.0, + "totduration": 1400.0 + }, + { + "delay": 0.0, + "amp": 0.1024, + "duration": 1400.0, + "totduration": 1400.0 + } + ] + }, + "IDthresh-sub_397": { + "stimuli": [ + { + "delay": 700.0, + "amp": 0.3231, + "duration": 2000.0, + "totduration": 3000.0 + }, + { + "delay": 0.0, + "amp": 0.1024, + "duration": 3000.0, + "totduration": 3000.0 + } + ] + }, + "IDthresh_399": { + "stimuli": [ + { + "delay": 700.0, + "amp": 0.3423, + "duration": 2000.0, + "totduration": 3000.0 + }, + { + "delay": 0.0, + "amp": 0.1025, + "duration": 3000.0, + "totduration": 3000.0 + } + ] + }, + "IDthresh_400": { + "stimuli": [ + { + "delay": 700.0, + "amp": 0.3519, + "duration": 2000.0, + "totduration": 3000.0 + }, + { + "delay": 0.0, + "amp": 0.1024, + "duration": 3000.0, + "totduration": 3000.0 + } + ] + }, + "IDthresh_409": { + "stimuli": [ + { + "delay": 700.0, + "amp": 0.4375, + "duration": 2000.0, + "totduration": 3000.0 + }, + { + "delay": 0.0, + "amp": 0.1024, + "duration": 3000.0, + "totduration": 3000.0 + } + ] + } +} diff --git a/examples/example_models/example-option-2/hall_of_fame.json b/examples/example_models/str-dspn-model1/hall_of_fame.json similarity index 100% rename from examples/example_models/example-option-2/hall_of_fame.json rename to examples/example_models/str-dspn-model1/hall_of_fame.json diff --git a/examples/example_models/str-dspn-model1/mechanisms/Im_ms.mod b/examples/example_models/str-dspn-model1/mechanisms/Im_ms.mod new file mode 100644 index 00000000..68313240 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/Im_ms.mod @@ -0,0 +1,104 @@ +COMMENT +Mechanism taken from Doron et al., 2017 +https://senselab.med.yale.edu/ModelDB/ShowModel.cshtml?model=231427&file=/reproduction/Im.mod#tabs-2 + +Reference : Adams et al. 1982 - M-currents and other potassium currents in bullfrog sympathetic neurones + +corrected rates using q10 = 2.3, target temperature 34, orginal 21 + +--------------------------------------------------------------- + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + +NEURON { + SUFFIX Im_ms + USEION k READ ek WRITE ik + RANGE gbar, gIm, ik + RANGE modACh, maxModACh, levelACh +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + modACh = 0 + maxModACh = 1 + levelACh = 0 +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + gIm (S/cm2) + mInf + mTau + mAlpha + mBeta +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + gIm = gbar*m*modulationACh() + ik = gIm*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((34-21)/10) + + UNITSOFF + mAlpha = 3.3e-3*exp(2.5*0.04*(v - -35)) + mBeta = 3.3e-3*exp(-2.5*0.04*(v - -35)) + mInf = mAlpha/(mAlpha + mBeta) + mTau = (1/(mAlpha + mBeta))/qt + UNITSON +} + +FUNCTION modulationACh() { + : returns modulation factor + + modulationACh = 1 + modACh*(maxModACh-1)*levelACh +} diff --git a/examples/example_models/str-dspn-model1/mechanisms/bk_ms.mod b/examples/example_models/str-dspn-model1/mechanisms/bk_ms.mod new file mode 100644 index 00000000..93dd5655 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/bk_ms.mod @@ -0,0 +1,104 @@ +TITLE BK-type calcium activated K channel (KCa1.1) + +UNITS { + (molar) = (1/liter) + (mV) = (millivolt) + (mA) = (milliamp) + (mM) = (millimolar) + FARADAY = (faraday) (kilocoulombs) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX bk_ms + USEION ca READ cai + USEION k READ ek WRITE ik + RANGE gbar, ik +} + +PARAMETER { + gbar = 0.0 (mho/cm2) + k1 = 0.003 (mM) + k4 = 0.009 (mM) + d1 = 0.84 + d4 = 1.0 + q = 1 : body temperature 35 C +} + +ASSIGNED { + v (mV) + ik (mA/cm2) + celsius (degC) + cai (mM) + ek (mV) + oinf + otau (ms) +} + +STATE { o } + +BREAKPOINT { + SOLVE state METHOD cnexp + ik = gbar*o*(v-ek) +} + +DERIVATIVE state { + rate(v, cai) + o' = (oinf-o)/otau*q +} + +INITIAL { + rate(v, cai) + o = oinf +} + +PROCEDURE rate(v (mV), ca (mM)) { + LOCAL a, b, sum, z + UNITSOFF + z = 1e-3*2*FARADAY/(R*(celsius+273.15)) + a = 0.48*ca/(ca+k1*exp(-z*d1*v)) + b = 0.28/(1+ca/(k4*exp(-z*d4*v))) + sum = a+b + oinf = a/sum + otau = 1/sum + UNITSON +} + +COMMENT + +Experimental data was obtained from BKCa channels from rat brain injected +as cRNAs into Xenopus oocytes [1]. Electrophysiological recordings were +performed at room temperature 22-24 C [1, supporting online material]. + +Original model [2, model 3 in Tab.1] was implemented by De Schutter +and adapted by Kai Du [5]. In the model revisions [3,4] parameters k1 and k4 +[2, channel A in Tab.2] were adjusted to fit rat/Xenopus data +[1, Fig.3C and Fig.4A, 10 uM Ca] at body temperature 35 C. + +NEURON implementation by Alexander Kozlov . + +[1] Berkefeld H, Sailer CA, Bildl W, Rohde V, Thumfart JO, Eble S, +Klugbauer N, Reisinger E, Bischofberger J, Oliver D, Knaus HG, Schulte U, +Fakler B (2006) BKCa-Cav channel complexes mediate rapid and localized +Ca2+-activated K+ signaling. Science 314(5799):615-20. + +[2] Moczydlowski E, Latorre R (1983) Gating kinetics of Ca2+-activated K+ +channels from rat muscle incorporated into planar lipid bilayers. Evidence +for two voltage-dependent Ca2+ binding reactions. J Gen Physiol +82(4):511-42. + +[3] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH, +Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on +calcium influx and spike timing-dependent plasticity in striatal medium +spiny neurons. PLoS Comput Biol 8(4):e1002493. + +[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +[5] Du K, Wu YW, Lindroos R, Liu Y, Rózsa B, Katona G, Ding JB, +Kotaleski JH (2017) Cell-type-specific inhibition of the dendritic +plateau potential in striatal spiny projection neurons. Proc Natl Acad +Sci USA 114:E7612-E7621. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model1/mechanisms/cadyn_ms.mod b/examples/example_models/str-dspn-model1/mechanisms/cadyn_ms.mod new file mode 100644 index 00000000..472b2de9 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/cadyn_ms.mod @@ -0,0 +1,67 @@ +TITLE Calcium dynamics for N, P/Q, R calcium pool + +NEURON { + SUFFIX cadyn_ms + USEION ca READ ica, cai WRITE cai VALENCE 2 + RANGE pump, cainf, taur, drive, depth +} + +UNITS { + (molar) = (1/liter) + (mM) = (millimolar) + (um) = (micron) + (mA) = (milliamp) + (msM) = (ms mM) + FARADAY = (faraday) (coulomb) +} + +PARAMETER { + drive = 10000 (1) + depth = 0.2 (um) + cainf = 70e-6 (mM) + taur = 43 (ms) + kt = 1e-4 (mM/ms) + kd = 1e-4 (mM) + pump = 0.02 +} + +STATE { cai (mM) } + +INITIAL { cai = cainf } + +ASSIGNED { + ica (mA/cm2) + drive_channel (mM/ms) + drive_pump (mM/ms) +} + +BREAKPOINT { + SOLVE state METHOD cnexp +} + +DERIVATIVE state { + + : force concentration to stay above cainf by only pumping if larger + drive_channel = -drive*ica/(2*FARADAY*depth) + drive_pump = -kt*(cai-cainf)/(cai+kd) + + if (drive_channel <= 0.) { drive_channel = 0. } + + cai' = drive_channel + pump*drive_pump + (cainf-cai)/taur +} + +COMMENT + +Original NEURON model by Wolf (2005) and Destexhe (1992). Adaptation by +Alexander Kozlov . Updated by Robert Lindroos . + +Updates by RL: +-cainf changed from 10 to 70 nM (sabatini et al., 2002 The Life Cycle of Ca 2+ Ions in Dendritic Spines) +-pump updated to only be active if cai > cainf + +[1] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model1/mechanisms/cal12_ms.mod b/examples/example_models/str-dspn-model1/mechanisms/cal12_ms.mod new file mode 100644 index 00000000..31e29e8d --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/cal12_ms.mod @@ -0,0 +1,176 @@ +TITLE HVA L-type calcium current (Cav1.2) + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (S) = (siemens) + (molar) = (1/liter) + (mM) = (millimolar) + FARADAY = (faraday) (coulomb) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX cal12_ms + USEION cal READ cali, calo WRITE ical VALENCE 2 + RANGE pbar, ical + RANGE modDA, maxModDA, levelDA + RANGE modACh, maxModACh, levelACh +} + +PARAMETER { + pbar = 0.0 (cm/s) + a = 0.17 + :q = 1 : room temperature 22-25 C + q = 2 : body temperature 35 C + modDA = 0 + maxModDA = 1 + levelDA = 0 + modACh = 0 + maxModACh = 1 + levelACh = 0 + +} + +ASSIGNED { + v (mV) + ical (mA/cm2) + ecal (mV) + celsius (degC) + cali (mM) + calo (mM) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + ical = pbar*m*(h*a+1-a)*ghk(v, cali, calo)*modulationDA()*modulationACh() +} + +INITIAL { + rates() + m = minf + h = hinf +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-8.9))/(-6.7))) + mtau = 0.06+1/(exp((v-10)/20)+exp((v-(-17))/-48)) + hinf = 1/(1+exp((v-(-13.4))/11.9)) + htau = 44.3 + UNITSON +} + +FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) { + LOCAL z, eci, eco + z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15)) + eco = co*efun(z) + eci = ci*efun(-z) + ghk = (1e-3)*2*FARADAY*(eci-eco) +} + +FUNCTION efun(z) { + if (fabs(z) < 1e-4) { + efun = 1-z/2 + }else{ + efun = z/(exp(z)-1) + } +} + + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + +FUNCTION modulationACh() { + : returns modulation factor + + modulationACh = 1 + modACh*(maxModACh-1)*levelACh +} + + +COMMENT + +Activation curve was reconstructed for cultured NAc neurons from P5-P32 +Charles River rat pups [1]. Activation time constant is from the +rodent neuron culture (both rat and mouse cells), room temperature 22-25 +C [2, Fig.15A]. Inactivation curve of CaL v1.3 current was taken from HEK +cells [3, Fig.2 and p.819] at room temperature. + +Original NEURON model by Wolf (2005) [4] was modified by Alexander Kozlov +. Kinetics of m1h type was used [5,6]. Activation +time constant was refitted to avoid singularity. + +[1] Churchill D, Macvicar BA (1998) Biophysical and pharmacological +characterization of voltage-dependent Ca2+ channels in neurons isolated +from rat nucleus accumbens. J Neurophysiol 79(2):635-47. + +[2] Kasai H, Neher E (1992) Dihydropyridine-sensitive and +omega-conotoxin-sensitive calcium channels in a mammalian +neuroblastoma-glioma cell line. J Physiol 448:161-88. + +[3] Bell DC, Butcher AJ, Berrow NS, Page KM, Brust PF, Nesterova A, +Stauderman KA, Seabrook GR, Nurnberg B, Dolphin AC (2001) Biophysical +properties, pharmacology, and modulation of human, neuronal L-type +(alpha(1D), Ca(V)1.3) voltage-dependent calcium currents. J Neurophysiol +85:816-827. + +[4] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[5] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH, +Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on +calcium influx and spike timing-dependent plasticity in striatal medium +spiny neurons. PLoS Comput Biol 8(4):e1002493. + +[6] Tuckwell HC (2012) Quantitative aspects of L-type Ca2+ currents. Prog +Neurobiol 96(1):1-31. + +add justification for modulation + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model1/mechanisms/cal13_ms.mod b/examples/example_models/str-dspn-model1/mechanisms/cal13_ms.mod new file mode 100644 index 00000000..cf4da3a3 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/cal13_ms.mod @@ -0,0 +1,178 @@ +TITLE LVA L-type calcium current (Cav1.3) + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (S) = (siemens) + (molar) = (1/liter) + (mM) = (millimolar) + FARADAY = (faraday) (coulomb) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX cal13_ms + USEION cal READ cali, calo WRITE ical VALENCE 2 + RANGE pbar, ical + RANGE modDA, maxModDA, levelDA + RANGE modACh, maxModACh, levelACh +} + +PARAMETER { + pbar = 0.0 (cm/s) + :q = 1 : room temperature 22-25 C + q = 2 : body temperature 35 C + modDA = 0 + maxModDA = 1 + levelDA = 0 + modACh = 0 + maxModACh = 1 + levelACh = 0 +} + +ASSIGNED { + v (mV) + ical (mA/cm2) + ecal (mV) + celsius (degC) + cali (mM) + calo (mM) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + ical = pbar*m*m*h*ghk(v, cali, calo)*modulationDA()*modulationACh() +} + +INITIAL { + rates() + m = minf + h = hinf +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-33))/(-6.7))) + mtau = 0.06+1/(exp((v-10)/20)+exp((v-(-17))/-48)) + hinf = 1/(1+exp((v-(-13.4))/11.9)) + htau = 44.3 + UNITSON +} + +FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) { + LOCAL z, eci, eco + z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15)) + eco = co*efun(z) + eci = ci*efun(-z) + ghk = (1e-3)*2*FARADAY*(eci-eco) +} + +FUNCTION efun(z) { + if (fabs(z) < 1e-4) { + efun = 1-z/2 + }else{ + efun = z/(exp(z)-1) + } +} + + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + +FUNCTION modulationACh() { + : returns modulation factor + + modulationACh = 1 + modACh*(maxModACh-1)*levelACh +} + + +COMMENT + +Activation curve was reconstructed for cultured NAc neurons from +P5-P32 Charles River rat pups [1] and shifted to match LVA data [7, +Fig.1D]. Activation time constant is from the rodent neuron culture (both +rat and mouse cells), room temperature 22-25 C [2, Fig.15A]. Inactivation +curve of CaL v1.3 current was taken from HEK cells [3, Fig.2 and p.819] +at room temperature. + +Original NEURON model by Wolf (2005) [4] was modified by Alexander Kozlov +. Kinetics of m2h type was used [5,6]. Activation +time constant was refitted to avoid singularity. + +[1] Churchill D, Macvicar BA (1998) Biophysical and pharmacological +characterization of voltage-dependent Ca2+ channels in neurons isolated +from rat nucleus accumbens. J Neurophysiol 79(2):635-47. + +[2] Kasai H, Neher E (1992) Dihydropyridine-sensitive and +omega-conotoxin-sensitive calcium channels in a mammalian +neuroblastoma-glioma cell line. J Physiol 448:161-88. + +[3] Bell DC, Butcher AJ, Berrow NS, Page KM, Brust PF, Nesterova A, +Stauderman KA, Seabrook GR, Nurnberg B, Dolphin AC (2001) Biophysical +properties, pharmacology, and modulation of human, neuronal L-type +(alpha(1D), Ca(V)1.3) voltage-dependent calcium currents. J Neurophysiol +85:816-827. + +[4] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[5] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH, +Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on +calcium influx and spike timing-dependent plasticity in striatal medium +spiny neurons. PLoS Comput Biol 8(4):e1002493. + +[6] Tuckwell HC (2012) Quantitative aspects of L-type Ca2+ currents. Prog +Neurobiol 96(1):1-31. + +[7] Xu W, Lipscombe D (2001) Neuronal cav1.3 L-type channels activate +at relatively hyperpolarized membrane potentials and are incompletely +inhibited by dihydropyridines. J Neurosci 21(16): 5944-5951. + + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model1/mechanisms/caldyn_ms.mod b/examples/example_models/str-dspn-model1/mechanisms/caldyn_ms.mod new file mode 100644 index 00000000..ac6484f4 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/caldyn_ms.mod @@ -0,0 +1,68 @@ +TITLE Calcium dynamics for L and T calcium pool + +NEURON { + SUFFIX caldyn_ms + USEION cal READ ical, cali WRITE cali VALENCE 2 + RANGE pump, cainf, taur, drive, depth +} + +UNITS { + (molar) = (1/liter) + (mM) = (millimolar) + (um) = (micron) + (mA) = (milliamp) + (msM) = (ms mM) + FARADAY = (faraday) (coulomb) +} + +PARAMETER { + drive = 10000 (1) + depth = 0.2 (um) + cainf = 70e-6 (mM) + taur = 43 (ms) + kt = 1e-4 (mM/ms) + kd = 1e-4 (mM) + pump = 0.02 +} + +STATE { cali (mM) } + +INITIAL { cali = cainf } + +ASSIGNED { + ical (mA/cm2) + drive_channel (mM/ms) + drive_pump (mM/ms) +} + +BREAKPOINT { + SOLVE state METHOD cnexp +} + + +DERIVATIVE state { + + : force concentration to stay above cainf by only pumping if larger + drive_channel = -drive*ical/(2*FARADAY*depth) + drive_pump = -kt*(cali-cainf)/(cali+kd) + + if (drive_channel <= 0.) { drive_channel = 0. } + + cali' = drive_channel + pump*drive_pump + (cainf-cali)/taur +} + +COMMENT + +Original NEURON model by Wolf (2005) and Destexhe (1992). Adaptation by +Alexander Kozlov . Updated by Robert Lindroos . + +Updates by RL: +-cainf changed from 10 to 70 nM (sabatini et al., 2002 The Life Cycle of Ca 2+ Ions in Dendritic Spines) +-pump updated to only be active if cai > cainf (neutralized by adding reversed entity) + +[1] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model1/mechanisms/can_ms.mod b/examples/example_models/str-dspn-model1/mechanisms/can_ms.mod new file mode 100644 index 00000000..8243d1c0 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/can_ms.mod @@ -0,0 +1,170 @@ +TITLE N-type calcium current (Cav2.2) + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (S) = (siemens) + (molar) = (1/liter) + (mM) = (millimolar) + FARADAY = (faraday) (coulomb) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX can_ms + USEION ca READ cai, cao WRITE ica VALENCE 2 + RANGE pbar, ica + RANGE modDA, maxModDA, levelDA, modACh, maxModACh, levelACh +} + +PARAMETER { + pbar = 0.0 (cm/s) + a = 0.21 + :q = 1 : room temperature 22-25 C + q = 3 : body temperature 35 C + modDA = 0 + maxModDA = 1 + levelDA = 0 + modACh = 0 + maxModACh = 1 + levelACh = 0 +} + +ASSIGNED { + v (mV) + ica (mA/cm2) + eca (mV) + celsius (degC) + cai (mM) + cao (mM) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + ica = pbar*m*m*(h*a+1-a)*ghk(v, cai, cao)*modulationDA()*modulationACh() +} + +INITIAL { + rates() + m = minf + h = hinf +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-3))/(-8))) + mtau = 0.06+1/(exp((v-25)/18)+exp((v-(-31))/(-44))) + hinf = 1/(1+exp((v-(-74.8))/6.5)) + htau = 70 + UNITSON +} + +FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) { + LOCAL z, eci, eco + z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15)) + eco = co*efun(z) + eci = ci*efun(-z) + ghk = (1e-3)*2*FARADAY*(eci-eco) +} + +FUNCTION efun(z) { + if (fabs(z) < 1e-4) { + efun = 1-z/2 + }else{ + efun = z/(exp(z)-1) + } +} + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + +FUNCTION modulationACh() { + : returns modulation factor + + modulationACh = 1 + modACh*(maxModACh-1)*levelACh +} + + +COMMENT + +Model is based on mixed data. Activation curve is from neostriatal +medium spiny neurons of adult P28+ rats [1, Fig.12F], unspecified +recording temperature. Potentials were not corrected for the liquid +junction potential, which was estimated to be 7 mV. Activation time +constant is from the rodent neuron culture (both rat and mouse cells), +room temperature 22-25 C [2, Fig.15B]. Inactivation data is from human +(HEK) cells [3, Tab.1, Tab.2], supposedly at room temperature. + +Kinetics of m2h type is used [2, Fig.5]. Activation of m^2 is fitted +to the experimental data [1,4], activation time constant corresponds +to m^2 already [2]. Original model [5,4] was modified by Alexander +Kozlov . Activation time constant was refitted to avoid +singularity in the expression. Temperature correction factor 3 is used +for body temperature [4,5]. + +[1] Bargas J, Howe A, Eberwine J, Cao Y, Surmeier DJ (1994) Cellular +and molecular characterization of Ca2+ currents in acutely isolated, +adult rat neostriatal neurons. J Neurosci 14(11 Pt 1):6667-86. + +[2] Kasai H, Neher E (1992) Dihydropyridine-sensitive and +omega-conotoxin-sensitive calcium channels in a mammalian +neuroblastoma-glioma cell line. J Physiol 448:161-88. + +[3] McNaughton NC, Randall AD (1997) Electrophysiological properties of +the human N-type Ca2+ channel: I. Channel gating in Ca2+, Ba2+ and Sr2+ +containing solutions. Neuropharmacology 36(7):895-915. + +[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +[5] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model1/mechanisms/caq_ms.mod b/examples/example_models/str-dspn-model1/mechanisms/caq_ms.mod new file mode 100644 index 00000000..357a1dc8 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/caq_ms.mod @@ -0,0 +1,105 @@ +TITLE Q-type calcium current (Cav2.1) + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (S) = (siemens) + (molar) = (1/liter) + (mM) = (millimolar) + FARADAY = (faraday) (coulomb) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX caq_ms + USEION ca READ cai, cao WRITE ica VALENCE 2 + RANGE pbar, ica +} + +PARAMETER { + pbar = 0.0 (cm/s) + :q = 1 : room temperature 22 C + q = 3 : body temperature 35 C +} + +ASSIGNED { + v (mV) + ica (mA/cm2) + eca (mV) + celsius (degC) + cai (mM) + cao (mM) + minf + mtau (ms) +} + +STATE { m } + +BREAKPOINT { + SOLVE states METHOD cnexp + ica = pbar*m*m*ghk(v, cai, cao) +} + +INITIAL { + rates() + m = minf +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-16.3))/(-7.9))) + mtau = 1.13*2 + UNITSON +} + +FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) { + LOCAL z, eci, eco + z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15)) + eco = co*efun(z) + eci = ci*efun(-z) + ghk = (1e-3)*2*FARADAY*(eci-eco) +} + +FUNCTION efun(z) { + if (fabs(z) < 1e-4) { + efun = 1-z/2 + }else{ + efun = z/(exp(z)-1) + } +} + +COMMENT + +Activation curve was reconstructed for cultured NAc neurons from P5-P32 +Charles River rat pups [1]. Activation time constant was measured in +culture neurons from cerebellum of P2-P5 rat pups [2] at room temperature +22 C. + +Original NEURON model by Wolf (2005) [3] was modified by Alexander Kozlov +. Activation curve was fitted to m2 kinetics [4], +activation time constant was scaled up as well. + +[1] Churchill D, Macvicar BA (1998) Biophysical and pharmacological +characterization of voltage-dependent Ca2+ channels in neurons isolated +from rat nucleus accumbens. J Neurophysiol 79(2):635-47. + +[2] Randall A, Tsien RW (1995) Pharmacological dissection of multiple +types of Ca2+ channel currents in rat cerebellar granule neurons. J +Neurosci 15(4):2995-3012. + +[3] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[4] Brown AM, Schwindt PC, Crill WE (1993) Voltage dependence and +activation kinetics of pharmacologically defined components of the +high-threshold calcium current in rat neocortical neurons. J Neurophysiol +70(4):1530-43. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model1/mechanisms/car_ms.mod b/examples/example_models/str-dspn-model1/mechanisms/car_ms.mod new file mode 100644 index 00000000..6a4b1413 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/car_ms.mod @@ -0,0 +1,154 @@ +TITLE R-type calcium current (Cav2.3) + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (S) = (siemens) + (molar) = (1/liter) + (mM) = (millimolar) + FARADAY = (faraday) (coulomb) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX car_ms + USEION ca READ cai, cao WRITE ica VALENCE 2 + RANGE pbar, ica + RANGE modDA, maxModDA, levelDA +} + +PARAMETER { + pbar = 0.0 (cm/s) + :q = 1 : room temperature 22 C + q = 3 : body temperature 35 C + modDA = 0 + maxModDA = 1 + levelDA = 0 +} + +ASSIGNED { + v (mV) + ica (mA/cm2) + eca (mV) + celsius (degC) + cai (mM) + cao (mM) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + ica = pbar*m*m*m*h*ghk(v, cai, cao)*modulationDA() +} + +INITIAL { + rates() + m = minf + h = hinf +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-29))/(-9.6))) + mtau = 5.1 + hinf = 1/(1+exp((v-(-33.3))/17)) + htau = 22+80/(1+exp((v-(-19))/5)) + UNITSON +} + +FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) { + LOCAL z, eci, eco + z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15)) + eco = co*efun(z) + eci = ci*efun(-z) + ghk = (1e-3)*2*FARADAY*(eci-eco) +} + +FUNCTION efun(z) { + if (fabs(z) < 1e-4) { + efun = 1-z/2 + }else{ + efun = z/(exp(z)-1) + } +} + + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + + +COMMENT + +Original data by Foehring et al (2000) [1] for dissociated MSNs from +P28-P42 Sprague-Dawley rat brain. Unspecified recording temperature. The +liquid junction potential was around 8 mV and was not corrected. Kinetics +of m3h type was fitted. Inactivation time constants were measured in +neurons from endopiriform nucleus of P7-P21 Hartley guinea pigs [2] +at room temperature 22 C. + +Original NEURON model by Wolf (2005) [3] modified by Alexander Kozlov +. Activation curve fitted to m^3 kinetics as in [4], +activation time constant matched m^3 originally [1]. Smooth fit of +inactivation time constant from [2,3]. + +[1] Foehring RC, Mermelstein PG, Song WJ, Ulrich S, Surmeier DJ +(2000) Unique properties of R-type calcium currents in neocortical and +neostriatal neurons. J Neurophysiol 84(5):2225-36. + +[2] Brevi S, de Curtis M, Magistretti J (2001) Pharmacological and +biophysical characterization of voltage-gated calcium currents in the +endopiriform nucleus of the guinea pig. J Neurophysiol 85(5):2076-87. + +[3] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model1/mechanisms/cat32_ms.mod b/examples/example_models/str-dspn-model1/mechanisms/cat32_ms.mod new file mode 100644 index 00000000..b76f310a --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/cat32_ms.mod @@ -0,0 +1,103 @@ +TITLE T-type calcium current (Cav3.2) + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (S) = (siemens) + (molar) = (1/liter) + (mM) = (millimolar) + FARADAY = (faraday) (coulomb) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX cat32_ms + USEION cal READ cali, calo WRITE ical VALENCE 2 + RANGE pbar, ical +} + +PARAMETER { + pbar = 0.0 (cm/s) + :q = 1 : room temperature 21 C + q = 3 : body temperature 35 C +} + +ASSIGNED { + v (mV) + ical (mA/cm2) + ecal (mV) + celsius (degC) + cali (mM) + calo (mM) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + ical = pbar*m*m*m*h*ghk(v, cali, calo) +} + +INITIAL { + rates() + m = minf + h = hinf +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-54))/(-7.8))) + mtau = (2.1+23/(1+exp((v-(-61))/6)))*3 + hinf = 1/(1+exp((v-(-64.2))/8.8)) + htau = 30+280/(1+exp((v-(-52))/7)) + UNITSON +} + +FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) { + LOCAL z, eci, eco + z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15)) + if(z == 0) { + z = z+1e-6 + } + eco = co*(z)/(exp(z)-1) + eci = ci*(-z)/(exp(-z)-1) + ghk = (1e-3)*2*FARADAY*(eci-eco) +} + +COMMENT + +Rat Cav3.2 channels were isolated and transfection of human embryonic +kidney cells was performed [1]. Electrophysiological recordings were +done in 21 C. + +NEURON model by Alexander Kozlov . Kinetics of m3h +type was used [2-4]. Activation time constant was scaled up accordingly. + +[1] Iftinca M, McKay BE, Snutch TP, McRory JE, Turner RW, Zamponi +GW (2006) Temperature dependence of T-type calcium channel +gating. Neuroscience 142(4):1031-42. + +[2] Crunelli V, Toth TI, Cope DW, Blethyn K, Hughes SW (2005) The +'window' T-type calcium current in brain dynamics of different behavioural +states. J Physiol 562(Pt 1):121-9. + +[3] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model1/mechanisms/cat33_ms.mod b/examples/example_models/str-dspn-model1/mechanisms/cat33_ms.mod new file mode 100644 index 00000000..7c2529d5 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/cat33_ms.mod @@ -0,0 +1,103 @@ +TITLE T-type calcium current (Cav3.3) + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (S) = (siemens) + (molar) = (1/liter) + (mM) = (millimolar) + FARADAY = (faraday) (coulomb) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX cat33_ms + USEION cal READ cali, calo WRITE ical VALENCE 2 + RANGE pbar, ical +} + +PARAMETER { + pbar = 0.0 (cm/s) + :q = 1 : room temperature 21 C + q = 3 : body temperature 35 C +} + +ASSIGNED { + v (mV) + ical (mA/cm2) + ecal (mV) + celsius (degC) + cali (mM) + calo (mM) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + ical = pbar*m*m*m*h*ghk(v, cali, calo) +} + +INITIAL { + rates() + m = minf + h = hinf +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-81))/(-5.8))) + mtau = (2.3+20/(1+exp((v-(-60))/9)))*3 + hinf = 1/(1+exp((v-(-78.3))/6.5)) + htau = 125+140/(1+exp((v-(-60))/3)) + UNITSON +} + +FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) { + LOCAL z, eci, eco + z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15)) + if(z == 0) { + z = z+1e-6 + } + eco = co*(z)/(exp(z)-1) + eci = ci*(-z)/(exp(-z)-1) + ghk = (1e-3)*2*FARADAY*(eci-eco) +} + +COMMENT + +Rat Cav3.2 channels were isolated and transfection of human embryonic +kidney cells was performed [1]. Electrophysiological recordings were +done in 21 C. + +NEURON model by Alexander Kozlov . Kinetics of m3h +type was used [2-4]. Activation time constant was scaled up accordingly. + +[1] Iftinca M, McKay BE, Snutch TP, McRory JE, Turner RW, Zamponi +GW (2006) Temperature dependence of T-type calcium channel +gating. Neuroscience 142(4):1031-42. + +[2] Crunelli V, Toth TI, Cope DW, Blethyn K, Hughes SW (2005) The +'window' T-type calcium current in brain dynamics of different behavioural +states. J Physiol 562(Pt 1):121-9. + +[3] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model1/mechanisms/kaf_ms.mod b/examples/example_models/str-dspn-model1/mechanisms/kaf_ms.mod new file mode 100644 index 00000000..71b01ea7 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/kaf_ms.mod @@ -0,0 +1,168 @@ +TITLE Fast A-type potassium current (Kv4.2) + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + + +NEURON { + SUFFIX kaf_ms + USEION k READ ek WRITE ik + RANGE gbar, gk, ik, q + RANGE modDA, maxModDA, levelDA + RANGE modACh, maxModACh, levelACh + RANGE modShift +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.0 (S/cm2) + q = 1 : room temperature (unspecified) + :q = 2 : body temperature 35 C (Du 2017) + :q = 3 : body temperature 35 C + modDA = 0 + maxModDA = 1 + levelDA = 0 + modShift = 0 + modACh = 0 + maxModACh = 1 + levelACh = 0 + + + +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + gk (S/cm2) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + gk = gbar*m*m*h*modulationDA() + modShift = modulationACh() + ik = gk*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +INITIAL { + rates() + m = minf + h = hinf +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-10+modShift))/(-17.7))) + mtau = 0.9+1.1/(1+exp((v-(-30))/10)) + hinf = 1/(1+exp((v-(-75.6))/11.8)) + htau = 14 + UNITSON + + :Du 2017 + :LOCAL alpha, beta, sum + :UNITSOFF + :alpha = 1.5/(1+exp((v-4)/(-17))) + :beta = 0.6/(1+exp((v-10)/9)) + :sum = alpha+beta + :minf = alpha/sum + :mtau = 1/sum + : + :alpha = 0.105/(1+exp((v-(-121))/22)) + :beta = 0.065/(1+exp((v-(-55))/(-11))) + :sum = alpha+beta + :hinf = alpha/sum + :htau = 1/sum + :UNITSON +} + + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + +FUNCTION modulationACh() { + : returns modulation factor + + modulationACh = 1 + modACh*(maxModACh-1)*levelACh +} + +COMMENT + +Original data by Tkatch et al (2000) [1]. Neostriatal neurons were acutely +dissociated from young adult rats, age P28-P42. Electrophysiological +recordings were done at unspecified temperature (room temperature 20-22 C +assumed). Potentials were not corrected for the liquid junction potential +(estimated 1-2 mV). + +Activation m^1 matches experimental data [1, Fig.2C]. Activation time +constants fit tabulated data [1, Fig.2B]. Slope of inactivation function +fitted to the data [1, Fig.3B] with half inactivation potential -75.6 +mV. Temperature factor q between 1.5 [3] and 3 [2] was used for body +temperature. Conductance kinetics of m2h type is used [2], no corrections +for m^2 applied. Later modification by Du [4] is close to this model. + +[1] Tkatch T, Baranauskas G, Surmeier DJ (2000) Kv4.2 mRNA abundance and +A-type K(+) current amplitude are linearly related in basal ganglia and +basal forebrain neurons. J Neurosci 20(2):579-88. + +[2] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[3] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH, +Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on +calcium influx and spike timing-dependent plasticity in striatal medium +spiny neurons. PLoS Comput Biol 8(4):e1002493. + +[4] Du K, Wu YW, Lindroos R, Liu Y, Rózsa B, Katona G, Ding JB, +Kotaleski JH (2017) Cell-type-specific inhibition of the dendritic +plateau potential in striatal spiny projection neurons. Proc Natl Acad +Sci USA 114:E7612-E7621. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model1/mechanisms/kas_ms.mod b/examples/example_models/str-dspn-model1/mechanisms/kas_ms.mod new file mode 100644 index 00000000..8b01bcee --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/kas_ms.mod @@ -0,0 +1,151 @@ +TITLE Slowly inactivating A-type potassium current (Kv1.2) + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + + +NEURON { + SUFFIX kas_ms + USEION k READ ek WRITE ik + RANGE gbar, gk, ik + RANGE modDA, maxModDA, levelDA + +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.0 (S/cm2) + a = 0.8 + :q = 1 : room temperature 22-24 C + q = 3 : body temperature 33 C + modDA = 0 + maxModDA = 1 + levelDA = 0 + +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + gk (S/cm2) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + gk = gbar*m*m*(h*a+1-a)*modulationDA() + ik = gk*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +INITIAL { + rates() + m = minf + h = hinf +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-27))/(-16))) + mtau = 3.4+89.2*exp(-((v-(-34.3))/30.1)^2) + hinf = 1/(1+exp((v-(-33.5))/21.5)) + htau = 548.7*6/(exp((v-(-96))/(-29.01))+exp((v-(-96))/100)) + : Du 2017 + :LOCAL alpha, beta, sum + :UNITSOFF + :alpha = 0.25/(1+exp((v-50)/(-20))) + :beta = 0.05/(1+exp((v-(-90))/35)) + :sum = alpha+beta + :minf = alpha/sum + :mtau = 1/sum + : + :alpha = 0.0025/(1+exp((v-(-95))/16)) + :beta = 0.002/(1+exp((v-50)/(-70))) + :sum = alpha+beta + :hinf = a+(alpha/sum)*(1-a) + :htau = 1/sum + UNITSON +} + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + + +COMMENT + +Experimental data by Shen et al (2004) [1]. Medium spiny neurons were +acutely dissociated from from young adult (P21-P28) Sprague-Dawley rat +brain. All recordings were conducted at 22-24 C. No correction for the +liquid junction potential was reported. + +Conductance kinetics of m2h type is used [1,2] with partial inactivation, +m2 (a h + (1-a)). Fraction a is set to 0.8, as in [1, Fig.6B]; other +values for a are possible [2] (see also kas.mod in companion code). +Equation for htau [1] is corrected to match the authors' data [1, Fig.6B]. +Time constants were corrected to body temperature with factor q=3 [1-3]. +Later modification by Du [4] is close to this model with adjusted +inactivation. + +[1] Shen W, Hernandez-Lopez S, Tkatch T, Held JE, Surmeier DJ (2004) +Kv1.2-containing K+ channels regulate subthreshold excitability of +striatal medium spiny neurons. J Neurophysiol 91(3):1337-49. + +[2] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[3] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +[4] Du K, Wu YW, Lindroos R, Liu Y, Rózsa B, Katona G, Ding JB, +Kotaleski JH (2017) Cell-type-specific inhibition of the dendritic +plateau potential in striatal spiny projection neurons. Proc Natl Acad +Sci USA 114:E7612-E7621. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model1/mechanisms/kdr_ms.mod b/examples/example_models/str-dspn-model1/mechanisms/kdr_ms.mod new file mode 100644 index 00000000..1a147689 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/kdr_ms.mod @@ -0,0 +1,79 @@ +TITLE Fast delayed rectifier potassium current (Kv3.1/3.2) + +NEURON { + SUFFIX kdr_ms + USEION k READ ek WRITE ik + RANGE gbar, gk, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.0 (S/cm2) + q = 1 +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + gk (S/cm2) + minf + mtau (ms) +} + +STATE { m } + +BREAKPOINT { + SOLVE states METHOD cnexp + gk = gbar*m + ik = gk*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q +} + +INITIAL { + rates() + m = minf +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-13))/(-6))) + mtau = 11.1 + UNITSON +} + +COMMENT + +Original data and model by Baranauskas et al (1999) [1] for globus +pallidus neurons from young adult rat. The temperature was not +specified. Potentials were not corrected for the liquid junction +potential, which was estimated to be 1-2 mV. + +Kinetics of m^1 type is used as in [2,3]. Room temperature 20-23 C +is assumed. + +NEURON implementation by Alexander Kozlov . + +[1] Baranauskas G, Tkatch T, Surmeier DJ (1999) Delayed rectifier currents +in rat globus pallidus neurons are attributable to Kv2.1 and Kv3.1/3.2 +K(+) channels. J Neurosci 19(15):6394-404. + +[2] Migliore M, Hoffman DA, Magee JC, Johnston D (1999) Role of an +A-type K+ conductance in the back-propagation of action potentials in the +dendrites of hippocampal pyramidal neurons. J Comput Neurosci 7(1):5-15. + +[3] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH, +Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on +calcium influx and spike timing-dependent plasticity in striatal medium +spiny neurons. PLoS Comput Biol 8(4):e1002493. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model1/mechanisms/kir_ms.mod b/examples/example_models/str-dspn-model1/mechanisms/kir_ms.mod new file mode 100644 index 00000000..5afd9e84 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/kir_ms.mod @@ -0,0 +1,147 @@ +TITLE Non-inactivating inwardly rectifying potassium current (Kir2.3) + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + + +NEURON { + SUFFIX kir_ms + USEION k READ ek WRITE ik + RANGE gbar, gk, ik, shift + RANGE modDA, maxModDA, levelDA + RANGE modACh, maxModACh, levelACh +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.0 (S/cm2) + shift = 0.0 (mV) + q = 1 : body temperature 35 C + modDA = 0 + maxModDA = 1 + levelDA = 0 + modACh = 0 + maxModACh = 1 + levelACh = 0 +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + gk (S/cm2) + minf + mtau (ms) +} + +STATE { m } + +BREAKPOINT { + SOLVE states METHOD cnexp + gk = gbar*m*modulationDA()*modulationACh() + ik = gk*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q +} + +INITIAL { + rates() + m = minf +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-82)-shift)/13)) + mtau = 1/(exp((v-(-103))/(-14.5))+0.125/(1+exp((v-(-35))/(-19)))) + UNITSON +} + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + +FUNCTION modulationACh() { + : returns modulation factor + + modulationACh = 1 + modACh*(maxModACh-1)*levelACh +} + +COMMENT + +Original model by Wolf et al (2005) [1] for the rat MSN cells from the +nucleus accumbens. The activation curve was fitted to a mouse Kir2.1 +channel expressed in HEK cells [2] and shifted to match extracellular +concentration of K in rat. Measured half-activation values are -109.3 +mV (striatonigral MSN) and -113.2 mV (striatopallidal MSN) [6, Supp +Tab.1]. Time constants were derived from Aplysia data [3] and adjusted +to match the rat experiments [1]. Time constant was further tuned [4] +to fit the rat data below -80 mV [5]. Kinetics is corrected to the body +temperature 35 C [4]. + +Non-inactivating Kir current was observed in cells expressing Kir2.2 +and/or Kir2.3 [5]. Activation variable with m^1 kinetics is used [1,4]. +Smooth fit of the time constants by Alexander Kozlov . + +[1] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[2] Kubo Y, Murata Y (2001) Control of rectification and permeation by +two distinct sites after the second transmembrane region in Kir2.1 K+ +channel. J Physiol 531, 645-660. + +[3] Hayashi H, Fishman HM (1988) Inward rectifier K+ channel kinetics +from analysis of the complex conductance of aplysia neuronal membrane. +Biophys J 53, 747-757. + +[4] Steephen JE, Manchanda R (2009) Differences in biophysical properties +of nucleus accumbens medium spiny neurons emerging from inactivation of +inward rectifying potassium currents. J Comput Neurosci 27(3):453-70 + +[5] Mermelstein PG, Song WJ, Tkatch T, Yan Z, Surmeier DJ (1998) +Inwardly rectifying potassium (IRK) currents are correlated with IRK +subunit expression in rat nucleus accumbens medium spiny neurons. J +Neurosci 18(17):6650-61. + +[6] Shen W, Tian X, Day M, Ulrich S, Tkatch T, Nathanson NM, Surmeier DJ +(2007) Cholinergic modulation of Kir2 channels selectively elevates +dendritic excitability in striatopallidal neurons. Nat Neurosci +10(11):1458-66. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model1/mechanisms/naf_ms.mod b/examples/example_models/str-dspn-model1/mechanisms/naf_ms.mod new file mode 100644 index 00000000..f676971f --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/naf_ms.mod @@ -0,0 +1,133 @@ +TITLE Fast transient sodium current + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + +NEURON { + SUFFIX naf_ms + USEION na READ ena WRITE ina + RANGE gbar, gna, ina + RANGE modDA, maxModDA, levelDA + RANGE modACh, maxModACh, levelACh +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.0 (S/cm2) + :q = 1 : room temperature 22 C + q = 1.8 : body temperature 35 C + modDA = 0 + maxModDA = 1 + levelDA = 0 + modACh = 0 + maxModACh = 1 + levelACh = 0 +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + gna (S/cm2) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + gna = gbar*m*m*m*h*modulationDA()*modulationACh() + ina = gna*(v-ena) +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +INITIAL { + rates() + m = minf + h = hinf +} + +PROCEDURE rates() { + UNITSOFF + :minf = 1/(1+exp((v-(-25.5))/(-9.2))) + :mtau = 0.33+1/(exp((v-(-62))/14)+exp((v-(-60))/(-17))) + :hinf = 1/(1+exp((v-(-63.2))/6)) + :htau = 0.6+1/(exp((v-(-44))/8)+exp((v-(-99))/(-44))) + minf = 1/(1+exp((v-(-25))/(-10))) + mtau = 0.33+1/(exp((v-(-62))/14)+exp((v-(-60))/(-17))) + hinf = 1/(1+exp((v-(-62))/6)) + htau = 0.6+1/(exp((v-(-44))/8)+exp((v-(-99))/(-44))) + UNITSON +} + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + +FUNCTION modulationACh() { + : returns modulation factor + + modulationACh = 1 + modACh*(maxModACh-1)*levelACh +} + +COMMENT + +Original data by Ogata and Tatebayashi (1990) [1]. Neostriatal neurons +of medium size (putative medium spiny neurons) freshly isolated from +the adult guinea pig brain (either sex, 200 g). Data compensated for +the liquid junction potential (-13 mV). Experiments carried out at room +temperature (22 C). Conductance fitted by m3h kinetics. + +Smooth fit of mtau and htau data [1] by Alexander Kozlov +assuming natural logarithm of tau values [1, Figs. 5 and 9] and +temperature correction factor of 1.8 [2] as suggested by Robert Lindroos +. + +[1] Ogata N, Tatebayashi H (1990) Sodium current kinetics in freshly +isolated neostriatal neurones of the adult guinea pig. Pflugers Arch +416(5):594-603. + +[2] Schwarz JR (1986) The effect of temperature on Na currents in rat +myelinated nerve fibres. Pflugers Arch. 406(4):397-404. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model1/mechanisms/sk_ms.mod b/examples/example_models/str-dspn-model1/mechanisms/sk_ms.mod new file mode 100644 index 00000000..c25fe7dc --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/sk_ms.mod @@ -0,0 +1,83 @@ +TITLE SK-type calcium activated K channel (KCa2.2) + +UNITS { + (molar) = (1/liter) + (mV) = (millivolt) + (mA) = (milliamp) + (mM) = (millimolar) +} + +NEURON { + SUFFIX sk_ms + USEION ca READ cai + USEION k READ ek WRITE ik + RANGE gbar, ik +} + +PARAMETER { + gbar = 0.0 (mho/cm2) + :q = 1 : room temperature + q = 1 : body temperature +} + +ASSIGNED { + v (mV) + ik (mA/cm2) + cai (mM) + ek (mV) + oinf + otau (ms) +} + +STATE { o } + +BREAKPOINT { + SOLVE state METHOD cnexp + ik = gbar*o*(v-ek) +} + +DERIVATIVE state { + rate(v, cai) + o' = (oinf-o)/otau*q +} + +INITIAL { + rate(v, cai) + o = oinf +} + +PROCEDURE rate(v (mV), ca (mM)) { + LOCAL a + a = (ca/0.57e-3)^5.2 + oinf = a/(1+a) + otau = 4.9 +} + +COMMENT + +Experimental data was obtained for the apamin-sensitive clone rSK2 from +rat brain cDNA expressed in Xenopus oocytes [1,2]. All experiments were +performed at room tempretaure. + +Original model [3] used calcium dependence from [2, Fig.2] and calcium +activation time constant from [1, Fig.13]. NEURON implementation by +Alexander Kozlov follows the revised model [4]. + +[1] Hirschberg B, Maylie J, Adelman JP, Marrion NV (1998) Gating of +recombinant small-conductance Ca-activated K+ channels by calcium. J +Gen Physiol 111(4):565-81. + +[2] Maylie J, Bond CT, Herson PS, Lee WS, Adelman JP (2004) Small +conductance Ca2+-activated K+ channels and calmodulin. J Physiol 554(Pt +2):255-61. + +[3] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH, +Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on +calcium influx and spike timing-dependent plasticity in striatal medium +spiny neurons. PLoS Comput Biol 8(4):e1002493. + +[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/Im_ms.c b/examples/example_models/str-dspn-model1/mechanisms/x86_64/Im_ms.c new file mode 100644 index 00000000..9e15a42a --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/x86_64/Im_ms.c @@ -0,0 +1,627 @@ +/* Created by Language version: 7.7.0 */ +/* VECTORIZED */ +#define NRN_VECTORIZED 1 +#include +#include +#include +#include "mech_api.h" +#undef PI +#define nil 0 +#include "md1redef.h" +#include "section.h" +#include "nrniv_mf.h" +#include "md2redef.h" + +#if METHOD3 +extern int _method3; +#endif + +#if !NRNGPU +#undef exp +#define exp hoc_Exp +extern double hoc_Exp(double); +#endif + +#define nrn_init _nrn_init__Im_ms +#define _nrn_initial _nrn_initial__Im_ms +#define nrn_cur _nrn_cur__Im_ms +#define _nrn_current _nrn_current__Im_ms +#define nrn_jacob _nrn_jacob__Im_ms +#define nrn_state _nrn_state__Im_ms +#define _net_receive _net_receive__Im_ms +#define rates rates__Im_ms +#define states states__Im_ms + +#define _threadargscomma_ _p, _ppvar, _thread, _nt, +#define _threadargsprotocomma_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, +#define _threadargs_ _p, _ppvar, _thread, _nt +#define _threadargsproto_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt + /*SUPPRESS 761*/ + /*SUPPRESS 762*/ + /*SUPPRESS 763*/ + /*SUPPRESS 765*/ + extern double *getarg(); + /* Thread safe. No static _p or _ppvar. */ + +#define t _nt->_t +#define dt _nt->_dt +#define gbar _p[0] +#define gbar_columnindex 0 +#define modACh _p[1] +#define modACh_columnindex 1 +#define maxModACh _p[2] +#define maxModACh_columnindex 2 +#define levelACh _p[3] +#define levelACh_columnindex 3 +#define ik _p[4] +#define ik_columnindex 4 +#define gIm _p[5] +#define gIm_columnindex 5 +#define m _p[6] +#define m_columnindex 6 +#define ek _p[7] +#define ek_columnindex 7 +#define mInf _p[8] +#define mInf_columnindex 8 +#define mTau _p[9] +#define mTau_columnindex 9 +#define mAlpha _p[10] +#define mAlpha_columnindex 10 +#define mBeta _p[11] +#define mBeta_columnindex 11 +#define Dm _p[12] +#define Dm_columnindex 12 +#define v _p[13] +#define v_columnindex 13 +#define _g _p[14] +#define _g_columnindex 14 +#define _ion_ek *_ppvar[0]._pval +#define _ion_ik *_ppvar[1]._pval +#define _ion_dikdv *_ppvar[2]._pval + +#if MAC +#if !defined(v) +#define v _mlhv +#endif +#if !defined(h) +#define h _mlhh +#endif +#endif + +#if defined(__cplusplus) +extern "C" { +#endif + static int hoc_nrnpointerindex = -1; + static Datum* _extcall_thread; + static Prop* _extcall_prop; + /* external NEURON variables */ + /* declaration of user functions */ + static void _hoc_modulationACh(void); + static void _hoc_rates(void); + static int _mechtype; +extern void _nrn_cacheloop_reg(int, int); +extern void hoc_register_prop_size(int, int, int); +extern void hoc_register_limits(int, HocParmLimits*); +extern void hoc_register_units(int, HocParmUnits*); +extern void nrn_promote(Prop*, int, int); +extern Memb_func* memb_func; + +#define NMODL_TEXT 1 +#if NMODL_TEXT +static const char* nmodl_file_text; +static const char* nmodl_filename; +extern void hoc_reg_nmodl_text(int, const char*); +extern void hoc_reg_nmodl_filename(int, const char*); +#endif + + extern void _nrn_setdata_reg(int, void(*)(Prop*)); + static void _setdata(Prop* _prop) { + _extcall_prop = _prop; + } + static void _hoc_setdata() { + Prop *_prop, *hoc_getdata_range(int); + _prop = hoc_getdata_range(_mechtype); + _setdata(_prop); + hoc_retpushx(1.); +} + /* connect user functions to hoc names */ + static VoidFunc hoc_intfunc[] = { + "setdata_Im_ms", _hoc_setdata, + "modulationACh_Im_ms", _hoc_modulationACh, + "rates_Im_ms", _hoc_rates, + 0, 0 +}; +#define modulationACh modulationACh_Im_ms + extern double modulationACh( _threadargsproto_ ); + /* declare global and static user variables */ + /* some parameters have upper and lower limits */ + static HocParmLimits _hoc_parm_limits[] = { + 0,0,0 +}; + static HocParmUnits _hoc_parm_units[] = { + "gbar_Im_ms", "S/cm2", + "ik_Im_ms", "mA/cm2", + "gIm_Im_ms", "S/cm2", + 0,0 +}; + static double delta_t = 0.01; + static double m0 = 0; + /* connect global user variables to hoc */ + static DoubScal hoc_scdoub[] = { + 0,0 +}; + static DoubVec hoc_vdoub[] = { + 0,0,0 +}; + static double _sav_indep; + static void nrn_alloc(Prop*); +static void nrn_init(NrnThread*, _Memb_list*, int); +static void nrn_state(NrnThread*, _Memb_list*, int); + static void nrn_cur(NrnThread*, _Memb_list*, int); +static void nrn_jacob(NrnThread*, _Memb_list*, int); + +static int _ode_count(int); +static void _ode_map(int, double**, double**, double*, Datum*, double*, int); +static void _ode_spec(NrnThread*, _Memb_list*, int); +static void _ode_matsol(NrnThread*, _Memb_list*, int); + +#define _cvode_ieq _ppvar[3]._i + static void _ode_matsol_instance1(_threadargsproto_); + /* connect range variables in _p that hoc is supposed to know about */ + static const char *_mechanism[] = { + "7.7.0", +"Im_ms", + "gbar_Im_ms", + "modACh_Im_ms", + "maxModACh_Im_ms", + "levelACh_Im_ms", + 0, + "ik_Im_ms", + "gIm_Im_ms", + 0, + "m_Im_ms", + 0, + 0}; + static Symbol* _k_sym; + +extern Prop* need_memb(Symbol*); + +static void nrn_alloc(Prop* _prop) { + Prop *prop_ion; + double *_p; Datum *_ppvar; + _p = nrn_prop_data_alloc(_mechtype, 15, _prop); + /*initialize range parameters*/ + gbar = 1e-05; + modACh = 0; + maxModACh = 1; + levelACh = 0; + _prop->param = _p; + _prop->param_size = 15; + _ppvar = nrn_prop_datum_alloc(_mechtype, 4, _prop); + _prop->dparam = _ppvar; + /*connect ionic variables to this model*/ + prop_ion = need_memb(_k_sym); + nrn_promote(prop_ion, 0, 1); + _ppvar[0]._pval = &prop_ion->param[0]; /* ek */ + _ppvar[1]._pval = &prop_ion->param[3]; /* ik */ + _ppvar[2]._pval = &prop_ion->param[4]; /* _ion_dikdv */ + +} + static void _initlists(); + /* some states have an absolute tolerance */ + static Symbol** _atollist; + static HocStateTolerance _hoc_state_tol[] = { + 0,0 +}; + static void _update_ion_pointer(Datum*); + extern Symbol* hoc_lookup(const char*); +extern void _nrn_thread_reg(int, int, void(*)(Datum*)); +extern void _nrn_thread_table_reg(int, void(*)(double*, Datum*, Datum*, NrnThread*, int)); +extern void hoc_register_tolerance(int, HocStateTolerance*, Symbol***); +extern void _cvode_abstol( Symbol**, double*, int); + + void _Im_ms_reg() { + int _vectorized = 1; + _initlists(); + ion_reg("k", -10000.); + _k_sym = hoc_lookup("k_ion"); + register_mech(_mechanism, nrn_alloc,nrn_cur, nrn_jacob, nrn_state, nrn_init, hoc_nrnpointerindex, 1); + _mechtype = nrn_get_mechtype(_mechanism[1]); + _nrn_setdata_reg(_mechtype, _setdata); + _nrn_thread_reg(_mechtype, 2, _update_ion_pointer); + #if NMODL_TEXT + hoc_reg_nmodl_text(_mechtype, nmodl_file_text); + hoc_reg_nmodl_filename(_mechtype, nmodl_filename); +#endif + hoc_register_prop_size(_mechtype, 15, 4); + hoc_register_dparam_semantics(_mechtype, 0, "k_ion"); + hoc_register_dparam_semantics(_mechtype, 1, "k_ion"); + hoc_register_dparam_semantics(_mechtype, 2, "k_ion"); + hoc_register_dparam_semantics(_mechtype, 3, "cvodeieq"); + hoc_register_cvode(_mechtype, _ode_count, _ode_map, _ode_spec, _ode_matsol); + hoc_register_tolerance(_mechtype, _hoc_state_tol, &_atollist); + hoc_register_var(hoc_scdoub, hoc_vdoub, hoc_intfunc); + ivoc_help("help ?1 Im_ms /home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/Im_ms.mod\n"); + hoc_register_limits(_mechtype, _hoc_parm_limits); + hoc_register_units(_mechtype, _hoc_parm_units); + } +static int _reset; +static char *modelname = ""; + +static int error; +static int _ninits = 0; +static int _match_recurse=1; +static void _modl_cleanup(){ _match_recurse=1;} +static int rates(_threadargsproto_); + +static int _ode_spec1(_threadargsproto_); +/*static int _ode_matsol1(_threadargsproto_);*/ + static int _slist1[1], _dlist1[1]; + static int states(_threadargsproto_); + +/*CVODE*/ + static int _ode_spec1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) {int _reset = 0; { + rates ( _threadargs_ ) ; + Dm = ( mInf - m ) / mTau ; + } + return _reset; +} + static int _ode_matsol1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + rates ( _threadargs_ ) ; + Dm = Dm / (1. - dt*( ( ( ( - 1.0 ) ) ) / mTau )) ; + return 0; +} + /*END CVODE*/ + static int states (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { { + rates ( _threadargs_ ) ; + m = m + (1. - exp(dt*(( ( ( - 1.0 ) ) ) / mTau)))*(- ( ( ( mInf ) ) / mTau ) / ( ( ( ( - 1.0 ) ) ) / mTau ) - m) ; + } + return 0; +} + +static int rates ( _threadargsproto_ ) { + double _lqt ; + _lqt = pow( 2.3 , ( ( 34.0 - 21.0 ) / 10.0 ) ) ; + mAlpha = 3.3e-3 * exp ( 2.5 * 0.04 * ( v - - 35.0 ) ) ; + mBeta = 3.3e-3 * exp ( - 2.5 * 0.04 * ( v - - 35.0 ) ) ; + mInf = mAlpha / ( mAlpha + mBeta ) ; + mTau = ( 1.0 / ( mAlpha + mBeta ) ) / _lqt ; + return 0; } + +static void _hoc_rates(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = 1.; + rates ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +double modulationACh ( _threadargsproto_ ) { + double _lmodulationACh; + _lmodulationACh = 1.0 + modACh * ( maxModACh - 1.0 ) * levelACh ; + +return _lmodulationACh; + } + +static void _hoc_modulationACh(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = modulationACh ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +static int _ode_count(int _type){ return 1;} + +static void _ode_spec(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + ek = _ion_ek; + _ode_spec1 (_p, _ppvar, _thread, _nt); + }} + +static void _ode_map(int _ieq, double** _pv, double** _pvdot, double* _pp, Datum* _ppd, double* _atol, int _type) { + double* _p; Datum* _ppvar; + int _i; _p = _pp; _ppvar = _ppd; + _cvode_ieq = _ieq; + for (_i=0; _i < 1; ++_i) { + _pv[_i] = _pp + _slist1[_i]; _pvdot[_i] = _pp + _dlist1[_i]; + _cvode_abstol(_atollist, _atol, _i); + } + } + +static void _ode_matsol_instance1(_threadargsproto_) { + _ode_matsol1 (_p, _ppvar, _thread, _nt); + } + +static void _ode_matsol(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + ek = _ion_ek; + _ode_matsol_instance1(_threadargs_); + }} + extern void nrn_update_ion_pointer(Symbol*, Datum*, int, int); + static void _update_ion_pointer(Datum* _ppvar) { + nrn_update_ion_pointer(_k_sym, _ppvar, 0, 0); + nrn_update_ion_pointer(_k_sym, _ppvar, 1, 3); + nrn_update_ion_pointer(_k_sym, _ppvar, 2, 4); + } + +static void initmodel(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + int _i; double _save;{ + m = m0; + { + rates ( _threadargs_ ) ; + m = mInf ; + } + +} +} + +static void nrn_init(NrnThread* _nt, _Memb_list* _ml, int _type){ +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v = _v; + ek = _ion_ek; + initmodel(_p, _ppvar, _thread, _nt); + } +} + +static double _nrn_current(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, double _v){double _current=0.;v=_v;{ { + gIm = gbar * m * modulationACh ( _threadargs_ ) ; + ik = gIm * ( v - ek ) ; + } + _current += ik; + +} return _current; +} + +static void nrn_cur(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; double _rhs, _v; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + ek = _ion_ek; + _g = _nrn_current(_p, _ppvar, _thread, _nt, _v + .001); + { double _dik; + _dik = ik; + _rhs = _nrn_current(_p, _ppvar, _thread, _nt, _v); + _ion_dikdv += (_dik - ik)/.001 ; + } + _g = (_g - _rhs)/.001; + _ion_ik += ik ; +#if CACHEVEC + if (use_cachevec) { + VEC_RHS(_ni[_iml]) -= _rhs; + }else +#endif + { + NODERHS(_nd) -= _rhs; + } + +} + +} + +static void nrn_jacob(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; +#if CACHEVEC + if (use_cachevec) { + VEC_D(_ni[_iml]) += _g; + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + NODED(_nd) += _g; + } + +} + +} + +static void nrn_state(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v = 0.0; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v=_v; +{ + ek = _ion_ek; + { states(_p, _ppvar, _thread, _nt); + } }} + +} + +static void terminal(){} + +static void _initlists(){ + double _x; double* _p = &_x; + int _i; static int _first = 1; + if (!_first) return; + _slist1[0] = m_columnindex; _dlist1[0] = Dm_columnindex; +_first = 0; +} + +#if defined(__cplusplus) +} /* extern "C" */ +#endif + +#if NMODL_TEXT +static const char* nmodl_filename = "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/Im_ms.mod"; +static const char* nmodl_file_text = + "COMMENT\n" + "Mechanism taken from Doron et al., 2017\n" + "https://senselab.med.yale.edu/ModelDB/ShowModel.cshtml?model=231427&file=/reproduction/Im.mod#tabs-2\n" + "\n" + "Reference : Adams et al. 1982 - M-currents and other potassium currents in bullfrog sympathetic neurones\n" + "\n" + "corrected rates using q10 = 2.3, target temperature 34, orginal 21\n" + "\n" + "---------------------------------------------------------------\n" + "\n" + "Neuromodulation is added as functions:\n" + " \n" + " modulationDA = 1 + modDA*(maxModDA-1)*levelDA\n" + "\n" + "where:\n" + " \n" + " modDA [0]: is a switch for turning modulation on or off {1/0}\n" + " maxModDA [1]: is the maximum modulation for this specific channel (read from the param file)\n" + " e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf}\n" + " levelDA [0]: is an additional parameter for scaling modulation. \n" + " Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1}\n" + " \n" + " Further neuromodulators can be added by for example:\n" + " modulationDA = 1 + modDA*(maxModDA-1)\n" + " modulationACh = 1 + modACh*(maxModACh-1)\n" + " ....\n" + "\n" + " etc. for other neuromodulators\n" + " \n" + " \n" + " \n" + "[] == default values\n" + "{} == ranges\n" + " \n" + "ENDCOMMENT\n" + "\n" + "NEURON {\n" + " SUFFIX Im_ms\n" + " USEION k READ ek WRITE ik\n" + " RANGE gbar, gIm, ik\n" + " RANGE modACh, maxModACh, levelACh\n" + "}\n" + "\n" + "UNITS {\n" + " (S) = (siemens)\n" + " (mV) = (millivolt)\n" + " (mA) = (milliamp)\n" + "}\n" + "\n" + "PARAMETER {\n" + " gbar = 0.00001 (S/cm2) \n" + " modACh = 0\n" + " maxModACh = 1 \n" + " levelACh = 0\n" + "}\n" + "\n" + "ASSIGNED {\n" + " v (mV)\n" + " ek (mV)\n" + " ik (mA/cm2)\n" + " gIm (S/cm2)\n" + " mInf\n" + " mTau\n" + " mAlpha\n" + " mBeta\n" + "}\n" + "\n" + "STATE { \n" + " m\n" + "}\n" + "\n" + "BREAKPOINT {\n" + " SOLVE states METHOD cnexp\n" + " gIm = gbar*m*modulationACh()\n" + " ik = gIm*(v-ek)\n" + "}\n" + "\n" + "DERIVATIVE states {\n" + " rates()\n" + " m' = (mInf-m)/mTau\n" + "}\n" + "\n" + "INITIAL{\n" + " rates()\n" + " m = mInf\n" + "}\n" + "\n" + "PROCEDURE rates(){\n" + " LOCAL qt\n" + " qt = 2.3^((34-21)/10)\n" + "\n" + " UNITSOFF\n" + " mAlpha = 3.3e-3*exp(2.5*0.04*(v - -35))\n" + " mBeta = 3.3e-3*exp(-2.5*0.04*(v - -35))\n" + " mInf = mAlpha/(mAlpha + mBeta)\n" + " mTau = (1/(mAlpha + mBeta))/qt\n" + " UNITSON\n" + "}\n" + "\n" + "FUNCTION modulationACh() {\n" + " : returns modulation factor\n" + " \n" + " modulationACh = 1 + modACh*(maxModACh-1)*levelACh \n" + "}\n" + ; +#endif diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/Im_ms.o b/examples/example_models/str-dspn-model1/mechanisms/x86_64/Im_ms.o new file mode 100644 index 00000000..56476f16 Binary files /dev/null and b/examples/example_models/str-dspn-model1/mechanisms/x86_64/Im_ms.o differ diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/bk_ms.c b/examples/example_models/str-dspn-model1/mechanisms/x86_64/bk_ms.c new file mode 100644 index 00000000..5d97552b --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/x86_64/bk_ms.c @@ -0,0 +1,628 @@ +/* Created by Language version: 7.7.0 */ +/* VECTORIZED */ +#define NRN_VECTORIZED 1 +#include +#include +#include +#include "mech_api.h" +#undef PI +#define nil 0 +#include "md1redef.h" +#include "section.h" +#include "nrniv_mf.h" +#include "md2redef.h" + +#if METHOD3 +extern int _method3; +#endif + +#if !NRNGPU +#undef exp +#define exp hoc_Exp +extern double hoc_Exp(double); +#endif + +#define nrn_init _nrn_init__bk_ms +#define _nrn_initial _nrn_initial__bk_ms +#define nrn_cur _nrn_cur__bk_ms +#define _nrn_current _nrn_current__bk_ms +#define nrn_jacob _nrn_jacob__bk_ms +#define nrn_state _nrn_state__bk_ms +#define _net_receive _net_receive__bk_ms +#define rate rate__bk_ms +#define state state__bk_ms + +#define _threadargscomma_ _p, _ppvar, _thread, _nt, +#define _threadargsprotocomma_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, +#define _threadargs_ _p, _ppvar, _thread, _nt +#define _threadargsproto_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt + /*SUPPRESS 761*/ + /*SUPPRESS 762*/ + /*SUPPRESS 763*/ + /*SUPPRESS 765*/ + extern double *getarg(); + /* Thread safe. No static _p or _ppvar. */ + +#define t _nt->_t +#define dt _nt->_dt +#define gbar _p[0] +#define gbar_columnindex 0 +#define ik _p[1] +#define ik_columnindex 1 +#define o _p[2] +#define o_columnindex 2 +#define cai _p[3] +#define cai_columnindex 3 +#define ek _p[4] +#define ek_columnindex 4 +#define oinf _p[5] +#define oinf_columnindex 5 +#define otau _p[6] +#define otau_columnindex 6 +#define Do _p[7] +#define Do_columnindex 7 +#define v _p[8] +#define v_columnindex 8 +#define _g _p[9] +#define _g_columnindex 9 +#define _ion_cai *_ppvar[0]._pval +#define _ion_ek *_ppvar[1]._pval +#define _ion_ik *_ppvar[2]._pval +#define _ion_dikdv *_ppvar[3]._pval + +#if MAC +#if !defined(v) +#define v _mlhv +#endif +#if !defined(h) +#define h _mlhh +#endif +#endif + +#if defined(__cplusplus) +extern "C" { +#endif + static int hoc_nrnpointerindex = -1; + static Datum* _extcall_thread; + static Prop* _extcall_prop; + /* external NEURON variables */ + extern double celsius; + /* declaration of user functions */ + static void _hoc_rate(void); + static int _mechtype; +extern void _nrn_cacheloop_reg(int, int); +extern void hoc_register_prop_size(int, int, int); +extern void hoc_register_limits(int, HocParmLimits*); +extern void hoc_register_units(int, HocParmUnits*); +extern void nrn_promote(Prop*, int, int); +extern Memb_func* memb_func; + +#define NMODL_TEXT 1 +#if NMODL_TEXT +static const char* nmodl_file_text; +static const char* nmodl_filename; +extern void hoc_reg_nmodl_text(int, const char*); +extern void hoc_reg_nmodl_filename(int, const char*); +#endif + + extern void _nrn_setdata_reg(int, void(*)(Prop*)); + static void _setdata(Prop* _prop) { + _extcall_prop = _prop; + } + static void _hoc_setdata() { + Prop *_prop, *hoc_getdata_range(int); + _prop = hoc_getdata_range(_mechtype); + _setdata(_prop); + hoc_retpushx(1.); +} + /* connect user functions to hoc names */ + static VoidFunc hoc_intfunc[] = { + "setdata_bk_ms", _hoc_setdata, + "rate_bk_ms", _hoc_rate, + 0, 0 +}; + /* declare global and static user variables */ +#define d4 d4_bk_ms + double d4 = 1; +#define d1 d1_bk_ms + double d1 = 0.84; +#define k4 k4_bk_ms + double k4 = 0.009; +#define k1 k1_bk_ms + double k1 = 0.003; +#define q q_bk_ms + double q = 1; + /* some parameters have upper and lower limits */ + static HocParmLimits _hoc_parm_limits[] = { + 0,0,0 +}; + static HocParmUnits _hoc_parm_units[] = { + "k1_bk_ms", "mM", + "k4_bk_ms", "mM", + "gbar_bk_ms", "mho/cm2", + "ik_bk_ms", "mA/cm2", + 0,0 +}; + static double delta_t = 0.01; + static double o0 = 0; + /* connect global user variables to hoc */ + static DoubScal hoc_scdoub[] = { + "k1_bk_ms", &k1_bk_ms, + "k4_bk_ms", &k4_bk_ms, + "d1_bk_ms", &d1_bk_ms, + "d4_bk_ms", &d4_bk_ms, + "q_bk_ms", &q_bk_ms, + 0,0 +}; + static DoubVec hoc_vdoub[] = { + 0,0,0 +}; + static double _sav_indep; + static void nrn_alloc(Prop*); +static void nrn_init(NrnThread*, _Memb_list*, int); +static void nrn_state(NrnThread*, _Memb_list*, int); + static void nrn_cur(NrnThread*, _Memb_list*, int); +static void nrn_jacob(NrnThread*, _Memb_list*, int); + +static int _ode_count(int); +static void _ode_map(int, double**, double**, double*, Datum*, double*, int); +static void _ode_spec(NrnThread*, _Memb_list*, int); +static void _ode_matsol(NrnThread*, _Memb_list*, int); + +#define _cvode_ieq _ppvar[4]._i + static void _ode_matsol_instance1(_threadargsproto_); + /* connect range variables in _p that hoc is supposed to know about */ + static const char *_mechanism[] = { + "7.7.0", +"bk_ms", + "gbar_bk_ms", + 0, + "ik_bk_ms", + 0, + "o_bk_ms", + 0, + 0}; + static Symbol* _ca_sym; + static Symbol* _k_sym; + +extern Prop* need_memb(Symbol*); + +static void nrn_alloc(Prop* _prop) { + Prop *prop_ion; + double *_p; Datum *_ppvar; + _p = nrn_prop_data_alloc(_mechtype, 10, _prop); + /*initialize range parameters*/ + gbar = 0; + _prop->param = _p; + _prop->param_size = 10; + _ppvar = nrn_prop_datum_alloc(_mechtype, 5, _prop); + _prop->dparam = _ppvar; + /*connect ionic variables to this model*/ + prop_ion = need_memb(_ca_sym); + nrn_promote(prop_ion, 1, 0); + _ppvar[0]._pval = &prop_ion->param[1]; /* cai */ + prop_ion = need_memb(_k_sym); + nrn_promote(prop_ion, 0, 1); + _ppvar[1]._pval = &prop_ion->param[0]; /* ek */ + _ppvar[2]._pval = &prop_ion->param[3]; /* ik */ + _ppvar[3]._pval = &prop_ion->param[4]; /* _ion_dikdv */ + +} + static void _initlists(); + /* some states have an absolute tolerance */ + static Symbol** _atollist; + static HocStateTolerance _hoc_state_tol[] = { + 0,0 +}; + static void _update_ion_pointer(Datum*); + extern Symbol* hoc_lookup(const char*); +extern void _nrn_thread_reg(int, int, void(*)(Datum*)); +extern void _nrn_thread_table_reg(int, void(*)(double*, Datum*, Datum*, NrnThread*, int)); +extern void hoc_register_tolerance(int, HocStateTolerance*, Symbol***); +extern void _cvode_abstol( Symbol**, double*, int); + + void _bk_ms_reg() { + int _vectorized = 1; + _initlists(); + ion_reg("ca", -10000.); + ion_reg("k", -10000.); + _ca_sym = hoc_lookup("ca_ion"); + _k_sym = hoc_lookup("k_ion"); + register_mech(_mechanism, nrn_alloc,nrn_cur, nrn_jacob, nrn_state, nrn_init, hoc_nrnpointerindex, 1); + _mechtype = nrn_get_mechtype(_mechanism[1]); + _nrn_setdata_reg(_mechtype, _setdata); + _nrn_thread_reg(_mechtype, 2, _update_ion_pointer); + #if NMODL_TEXT + hoc_reg_nmodl_text(_mechtype, nmodl_file_text); + hoc_reg_nmodl_filename(_mechtype, nmodl_filename); +#endif + hoc_register_prop_size(_mechtype, 10, 5); + hoc_register_dparam_semantics(_mechtype, 0, "ca_ion"); + hoc_register_dparam_semantics(_mechtype, 1, "k_ion"); + hoc_register_dparam_semantics(_mechtype, 2, "k_ion"); + hoc_register_dparam_semantics(_mechtype, 3, "k_ion"); + hoc_register_dparam_semantics(_mechtype, 4, "cvodeieq"); + hoc_register_cvode(_mechtype, _ode_count, _ode_map, _ode_spec, _ode_matsol); + hoc_register_tolerance(_mechtype, _hoc_state_tol, &_atollist); + hoc_register_var(hoc_scdoub, hoc_vdoub, hoc_intfunc); + ivoc_help("help ?1 bk_ms /home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/bk_ms.mod\n"); + hoc_register_limits(_mechtype, _hoc_parm_limits); + hoc_register_units(_mechtype, _hoc_parm_units); + } + +#define FARADAY _nrnunit_FARADAY[_nrnunit_use_legacy_] +static double _nrnunit_FARADAY[2] = {0x1.81f0fae775425p+6, 96.4853}; /* 96.4853321233100161 */ + +#define R _nrnunit_R[_nrnunit_use_legacy_] +static double _nrnunit_R[2] = {0x1.0a1013e8990bep+3, 8.3145}; /* 8.3144626181532395 */ +static int _reset; +static char *modelname = "BK-type calcium activated K channel (KCa1.1)"; + +static int error; +static int _ninits = 0; +static int _match_recurse=1; +static void _modl_cleanup(){ _match_recurse=1;} +static int rate(_threadargsprotocomma_ double, double); + +static int _ode_spec1(_threadargsproto_); +/*static int _ode_matsol1(_threadargsproto_);*/ + static int _slist1[1], _dlist1[1]; + static int state(_threadargsproto_); + +/*CVODE*/ + static int _ode_spec1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) {int _reset = 0; { + rate ( _threadargscomma_ v , cai ) ; + Do = ( oinf - o ) / otau * q ; + } + return _reset; +} + static int _ode_matsol1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + rate ( _threadargscomma_ v , cai ) ; + Do = Do / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / otau )*( q ) )) ; + return 0; +} + /*END CVODE*/ + static int state (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { { + rate ( _threadargscomma_ v , cai ) ; + o = o + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / otau )*( q ))))*(- ( ( ( ( oinf ) ) / otau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / otau )*( q ) ) - o) ; + } + return 0; +} + +static int rate ( _threadargsprotocomma_ double _lv , double _lca ) { + double _la , _lb , _lsum , _lz ; + _lz = 1e-3 * 2.0 * FARADAY / ( R * ( celsius + 273.15 ) ) ; + _la = 0.48 * _lca / ( _lca + k1 * exp ( - _lz * d1 * _lv ) ) ; + _lb = 0.28 / ( 1.0 + _lca / ( k4 * exp ( - _lz * d4 * _lv ) ) ) ; + _lsum = _la + _lb ; + oinf = _la / _lsum ; + otau = 1.0 / _lsum ; + return 0; } + +static void _hoc_rate(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = 1.; + rate ( _p, _ppvar, _thread, _nt, *getarg(1) , *getarg(2) ); + hoc_retpushx(_r); +} + +static int _ode_count(int _type){ return 1;} + +static void _ode_spec(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + cai = _ion_cai; + ek = _ion_ek; + _ode_spec1 (_p, _ppvar, _thread, _nt); + }} + +static void _ode_map(int _ieq, double** _pv, double** _pvdot, double* _pp, Datum* _ppd, double* _atol, int _type) { + double* _p; Datum* _ppvar; + int _i; _p = _pp; _ppvar = _ppd; + _cvode_ieq = _ieq; + for (_i=0; _i < 1; ++_i) { + _pv[_i] = _pp + _slist1[_i]; _pvdot[_i] = _pp + _dlist1[_i]; + _cvode_abstol(_atollist, _atol, _i); + } + } + +static void _ode_matsol_instance1(_threadargsproto_) { + _ode_matsol1 (_p, _ppvar, _thread, _nt); + } + +static void _ode_matsol(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + cai = _ion_cai; + ek = _ion_ek; + _ode_matsol_instance1(_threadargs_); + }} + extern void nrn_update_ion_pointer(Symbol*, Datum*, int, int); + static void _update_ion_pointer(Datum* _ppvar) { + nrn_update_ion_pointer(_ca_sym, _ppvar, 0, 1); + nrn_update_ion_pointer(_k_sym, _ppvar, 1, 0); + nrn_update_ion_pointer(_k_sym, _ppvar, 2, 3); + nrn_update_ion_pointer(_k_sym, _ppvar, 3, 4); + } + +static void initmodel(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + int _i; double _save;{ + o = o0; + { + rate ( _threadargscomma_ v , cai ) ; + o = oinf ; + } + +} +} + +static void nrn_init(NrnThread* _nt, _Memb_list* _ml, int _type){ +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v = _v; + cai = _ion_cai; + ek = _ion_ek; + initmodel(_p, _ppvar, _thread, _nt); + } +} + +static double _nrn_current(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, double _v){double _current=0.;v=_v;{ { + ik = gbar * o * ( v - ek ) ; + } + _current += ik; + +} return _current; +} + +static void nrn_cur(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; double _rhs, _v; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + cai = _ion_cai; + ek = _ion_ek; + _g = _nrn_current(_p, _ppvar, _thread, _nt, _v + .001); + { double _dik; + _dik = ik; + _rhs = _nrn_current(_p, _ppvar, _thread, _nt, _v); + _ion_dikdv += (_dik - ik)/.001 ; + } + _g = (_g - _rhs)/.001; + _ion_ik += ik ; +#if CACHEVEC + if (use_cachevec) { + VEC_RHS(_ni[_iml]) -= _rhs; + }else +#endif + { + NODERHS(_nd) -= _rhs; + } + +} + +} + +static void nrn_jacob(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; +#if CACHEVEC + if (use_cachevec) { + VEC_D(_ni[_iml]) += _g; + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + NODED(_nd) += _g; + } + +} + +} + +static void nrn_state(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v = 0.0; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v=_v; +{ + cai = _ion_cai; + ek = _ion_ek; + { state(_p, _ppvar, _thread, _nt); + } }} + +} + +static void terminal(){} + +static void _initlists(){ + double _x; double* _p = &_x; + int _i; static int _first = 1; + if (!_first) return; + _slist1[0] = o_columnindex; _dlist1[0] = Do_columnindex; +_first = 0; +} + +#if defined(__cplusplus) +} /* extern "C" */ +#endif + +#if NMODL_TEXT +static const char* nmodl_filename = "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/bk_ms.mod"; +static const char* nmodl_file_text = + "TITLE BK-type calcium activated K channel (KCa1.1)\n" + "\n" + "UNITS {\n" + " (molar) = (1/liter)\n" + " (mV) = (millivolt)\n" + " (mA) = (milliamp)\n" + " (mM) = (millimolar)\n" + " FARADAY = (faraday) (kilocoulombs)\n" + " R = (k-mole) (joule/degC)\n" + "}\n" + "\n" + "NEURON {\n" + " SUFFIX bk_ms\n" + " USEION ca READ cai\n" + " USEION k READ ek WRITE ik\n" + " RANGE gbar, ik\n" + "}\n" + "\n" + "PARAMETER {\n" + " gbar = 0.0 (mho/cm2)\n" + " k1 = 0.003 (mM)\n" + " k4 = 0.009 (mM)\n" + " d1 = 0.84\n" + " d4 = 1.0\n" + " q = 1 : body temperature 35 C\n" + "}\n" + "\n" + "ASSIGNED {\n" + " v (mV)\n" + " ik (mA/cm2)\n" + " celsius (degC)\n" + " cai (mM) \n" + " ek (mV)\n" + " oinf\n" + " otau (ms)\n" + "}\n" + "\n" + "STATE { o }\n" + "\n" + "BREAKPOINT {\n" + " SOLVE state METHOD cnexp\n" + " ik = gbar*o*(v-ek)\n" + "}\n" + "\n" + "DERIVATIVE state {\n" + " rate(v, cai)\n" + " o' = (oinf-o)/otau*q\n" + "}\n" + "\n" + "INITIAL {\n" + " rate(v, cai)\n" + " o = oinf\n" + "}\n" + "\n" + "PROCEDURE rate(v (mV), ca (mM)) {\n" + " LOCAL a, b, sum, z\n" + " UNITSOFF\n" + " z = 1e-3*2*FARADAY/(R*(celsius+273.15))\n" + " a = 0.48*ca/(ca+k1*exp(-z*d1*v))\n" + " b = 0.28/(1+ca/(k4*exp(-z*d4*v)))\n" + " sum = a+b\n" + " oinf = a/sum\n" + " otau = 1/sum\n" + " UNITSON\n" + "}\n" + "\n" + "COMMENT\n" + "\n" + "Experimental data was obtained from BKCa channels from rat brain injected\n" + "as cRNAs into Xenopus oocytes [1]. Electrophysiological recordings were\n" + "performed at room temperature 22-24 C [1, supporting online material].\n" + "\n" + "Original model [2, model 3 in Tab.1] was implemented by De Schutter\n" + "and adapted by Kai Du [5]. In the model revisions [3,4] parameters k1 and k4\n" + "[2, channel A in Tab.2] were adjusted to fit rat/Xenopus data\n" + "[1, Fig.3C and Fig.4A, 10 uM Ca] at body temperature 35 C.\n" + "\n" + "NEURON implementation by Alexander Kozlov .\n" + "\n" + "[1] Berkefeld H, Sailer CA, Bildl W, Rohde V, Thumfart JO, Eble S,\n" + "Klugbauer N, Reisinger E, Bischofberger J, Oliver D, Knaus HG, Schulte U,\n" + "Fakler B (2006) BKCa-Cav channel complexes mediate rapid and localized\n" + "Ca2+-activated K+ signaling. Science 314(5799):615-20.\n" + "\n" + "[2] Moczydlowski E, Latorre R (1983) Gating kinetics of Ca2+-activated K+\n" + "channels from rat muscle incorporated into planar lipid bilayers. Evidence\n" + "for two voltage-dependent Ca2+ binding reactions. J Gen Physiol\n" + "82(4):511-42.\n" + "\n" + "[3] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH,\n" + "Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on\n" + "calcium influx and spike timing-dependent plasticity in striatal medium\n" + "spiny neurons. PLoS Comput Biol 8(4):e1002493.\n" + "\n" + "[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of\n" + "spike timing-dependent calcium influx during corticostriatal upstates. J\n" + "Neurophysiol 110(7):1631-45.\n" + "\n" + "[5] Du K, Wu YW, Lindroos R, Liu Y, R\n" + "\n" + "zsa B, Katona G, Ding JB,\n" + "Kotaleski JH (2017) Cell-type-specific inhibition of the dendritic\n" + "plateau potential in striatal spiny projection neurons. Proc Natl Acad\n" + "Sci USA 114:E7612-E7621.\n" + "\n" + "ENDCOMMENT\n" + ; +#endif diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/bk_ms.o b/examples/example_models/str-dspn-model1/mechanisms/x86_64/bk_ms.o new file mode 100644 index 00000000..49cf11eb Binary files /dev/null and b/examples/example_models/str-dspn-model1/mechanisms/x86_64/bk_ms.o differ diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/cadyn_ms.c b/examples/example_models/str-dspn-model1/mechanisms/x86_64/cadyn_ms.c new file mode 100644 index 00000000..1c698fe1 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/x86_64/cadyn_ms.c @@ -0,0 +1,554 @@ +/* Created by Language version: 7.7.0 */ +/* VECTORIZED */ +#define NRN_VECTORIZED 1 +#include +#include +#include +#include "mech_api.h" +#undef PI +#define nil 0 +#include "md1redef.h" +#include "section.h" +#include "nrniv_mf.h" +#include "md2redef.h" + +#if METHOD3 +extern int _method3; +#endif + +#if !NRNGPU +#undef exp +#define exp hoc_Exp +extern double hoc_Exp(double); +#endif + +#define nrn_init _nrn_init__cadyn_ms +#define _nrn_initial _nrn_initial__cadyn_ms +#define nrn_cur _nrn_cur__cadyn_ms +#define _nrn_current _nrn_current__cadyn_ms +#define nrn_jacob _nrn_jacob__cadyn_ms +#define nrn_state _nrn_state__cadyn_ms +#define _net_receive _net_receive__cadyn_ms +#define state state__cadyn_ms + +#define _threadargscomma_ _p, _ppvar, _thread, _nt, +#define _threadargsprotocomma_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, +#define _threadargs_ _p, _ppvar, _thread, _nt +#define _threadargsproto_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt + /*SUPPRESS 761*/ + /*SUPPRESS 762*/ + /*SUPPRESS 763*/ + /*SUPPRESS 765*/ + extern double *getarg(); + /* Thread safe. No static _p or _ppvar. */ + +#define t _nt->_t +#define dt _nt->_dt +#define drive _p[0] +#define drive_columnindex 0 +#define depth _p[1] +#define depth_columnindex 1 +#define cainf _p[2] +#define cainf_columnindex 2 +#define taur _p[3] +#define taur_columnindex 3 +#define pump _p[4] +#define pump_columnindex 4 +#define cai _p[5] +#define cai_columnindex 5 +#define Dcai _p[6] +#define Dcai_columnindex 6 +#define ica _p[7] +#define ica_columnindex 7 +#define drive_channel _p[8] +#define drive_channel_columnindex 8 +#define drive_pump _p[9] +#define drive_pump_columnindex 9 +#define v _p[10] +#define v_columnindex 10 +#define _g _p[11] +#define _g_columnindex 11 +#define _ion_ica *_ppvar[0]._pval +#define _ion_cai *_ppvar[1]._pval +#define _style_ca *((int*)_ppvar[2]._pvoid) + +#if MAC +#if !defined(v) +#define v _mlhv +#endif +#if !defined(h) +#define h _mlhh +#endif +#endif + +#if defined(__cplusplus) +extern "C" { +#endif + static int hoc_nrnpointerindex = -1; + static Datum* _extcall_thread; + static Prop* _extcall_prop; + /* external NEURON variables */ + /* declaration of user functions */ + static int _mechtype; +extern void _nrn_cacheloop_reg(int, int); +extern void hoc_register_prop_size(int, int, int); +extern void hoc_register_limits(int, HocParmLimits*); +extern void hoc_register_units(int, HocParmUnits*); +extern void nrn_promote(Prop*, int, int); +extern Memb_func* memb_func; + +#define NMODL_TEXT 1 +#if NMODL_TEXT +static const char* nmodl_file_text; +static const char* nmodl_filename; +extern void hoc_reg_nmodl_text(int, const char*); +extern void hoc_reg_nmodl_filename(int, const char*); +#endif + + extern void _nrn_setdata_reg(int, void(*)(Prop*)); + static void _setdata(Prop* _prop) { + _extcall_prop = _prop; + } + static void _hoc_setdata() { + Prop *_prop, *hoc_getdata_range(int); + _prop = hoc_getdata_range(_mechtype); + _setdata(_prop); + hoc_retpushx(1.); +} + /* connect user functions to hoc names */ + static VoidFunc hoc_intfunc[] = { + "setdata_cadyn_ms", _hoc_setdata, + 0, 0 +}; + /* declare global and static user variables */ +#define kd kd_cadyn_ms + double kd = 0.0001; +#define kt kt_cadyn_ms + double kt = 0.0001; + /* some parameters have upper and lower limits */ + static HocParmLimits _hoc_parm_limits[] = { + 0,0,0 +}; + static HocParmUnits _hoc_parm_units[] = { + "kt_cadyn_ms", "mM/ms", + "kd_cadyn_ms", "mM", + "drive_cadyn_ms", "1", + "depth_cadyn_ms", "um", + "cainf_cadyn_ms", "mM", + "taur_cadyn_ms", "ms", + 0,0 +}; + static double cai0 = 0; + static double delta_t = 0.01; + /* connect global user variables to hoc */ + static DoubScal hoc_scdoub[] = { + "kt_cadyn_ms", &kt_cadyn_ms, + "kd_cadyn_ms", &kd_cadyn_ms, + 0,0 +}; + static DoubVec hoc_vdoub[] = { + 0,0,0 +}; + static double _sav_indep; + static void nrn_alloc(Prop*); +static void nrn_init(NrnThread*, _Memb_list*, int); +static void nrn_state(NrnThread*, _Memb_list*, int); + static void nrn_cur(NrnThread*, _Memb_list*, int); +static void nrn_jacob(NrnThread*, _Memb_list*, int); + +static int _ode_count(int); +static void _ode_map(int, double**, double**, double*, Datum*, double*, int); +static void _ode_spec(NrnThread*, _Memb_list*, int); +static void _ode_matsol(NrnThread*, _Memb_list*, int); + +#define _cvode_ieq _ppvar[3]._i + static void _ode_matsol_instance1(_threadargsproto_); + /* connect range variables in _p that hoc is supposed to know about */ + static const char *_mechanism[] = { + "7.7.0", +"cadyn_ms", + "drive_cadyn_ms", + "depth_cadyn_ms", + "cainf_cadyn_ms", + "taur_cadyn_ms", + "pump_cadyn_ms", + 0, + 0, + 0, + 0}; + static Symbol* _ca_sym; + +extern Prop* need_memb(Symbol*); + +static void nrn_alloc(Prop* _prop) { + Prop *prop_ion; + double *_p; Datum *_ppvar; + _p = nrn_prop_data_alloc(_mechtype, 12, _prop); + /*initialize range parameters*/ + drive = 10000; + depth = 0.2; + cainf = 7e-05; + taur = 43; + pump = 0.02; + _prop->param = _p; + _prop->param_size = 12; + _ppvar = nrn_prop_datum_alloc(_mechtype, 4, _prop); + _prop->dparam = _ppvar; + /*connect ionic variables to this model*/ + prop_ion = need_memb(_ca_sym); + nrn_check_conc_write(_prop, prop_ion, 1); + nrn_promote(prop_ion, 3, 0); + _ppvar[0]._pval = &prop_ion->param[3]; /* ica */ + _ppvar[1]._pval = &prop_ion->param[1]; /* cai */ + _ppvar[2]._pvoid = (void*)(&(prop_ion->dparam[0]._i)); /* iontype for ca */ + +} + static void _initlists(); + /* some states have an absolute tolerance */ + static Symbol** _atollist; + static HocStateTolerance _hoc_state_tol[] = { + 0,0 +}; + static void _update_ion_pointer(Datum*); + extern Symbol* hoc_lookup(const char*); +extern void _nrn_thread_reg(int, int, void(*)(Datum*)); +extern void _nrn_thread_table_reg(int, void(*)(double*, Datum*, Datum*, NrnThread*, int)); +extern void hoc_register_tolerance(int, HocStateTolerance*, Symbol***); +extern void _cvode_abstol( Symbol**, double*, int); + + void _cadyn_ms_reg() { + int _vectorized = 1; + _initlists(); + ion_reg("ca", 2.0); + _ca_sym = hoc_lookup("ca_ion"); + register_mech(_mechanism, nrn_alloc,nrn_cur, nrn_jacob, nrn_state, nrn_init, hoc_nrnpointerindex, 1); + _mechtype = nrn_get_mechtype(_mechanism[1]); + _nrn_setdata_reg(_mechtype, _setdata); + _nrn_thread_reg(_mechtype, 2, _update_ion_pointer); + #if NMODL_TEXT + hoc_reg_nmodl_text(_mechtype, nmodl_file_text); + hoc_reg_nmodl_filename(_mechtype, nmodl_filename); +#endif + hoc_register_prop_size(_mechtype, 12, 4); + hoc_register_dparam_semantics(_mechtype, 0, "ca_ion"); + hoc_register_dparam_semantics(_mechtype, 1, "ca_ion"); + hoc_register_dparam_semantics(_mechtype, 2, "#ca_ion"); + hoc_register_dparam_semantics(_mechtype, 3, "cvodeieq"); + nrn_writes_conc(_mechtype, 0); + hoc_register_cvode(_mechtype, _ode_count, _ode_map, _ode_spec, _ode_matsol); + hoc_register_tolerance(_mechtype, _hoc_state_tol, &_atollist); + hoc_register_var(hoc_scdoub, hoc_vdoub, hoc_intfunc); + ivoc_help("help ?1 cadyn_ms /home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/cadyn_ms.mod\n"); + hoc_register_limits(_mechtype, _hoc_parm_limits); + hoc_register_units(_mechtype, _hoc_parm_units); + } + +#define FARADAY _nrnunit_FARADAY[_nrnunit_use_legacy_] +static double _nrnunit_FARADAY[2] = {0x1.78e555060882cp+16, 96485.3}; /* 96485.3321233100141 */ +static int _reset; +static char *modelname = "Calcium dynamics for N, P/Q, R calcium pool"; + +static int error; +static int _ninits = 0; +static int _match_recurse=1; +static void _modl_cleanup(){ _match_recurse=1;} + +static int _ode_spec1(_threadargsproto_); +/*static int _ode_matsol1(_threadargsproto_);*/ + static int _slist1[1], _dlist1[1]; + static int state(_threadargsproto_); + +/*CVODE*/ + static int _ode_spec1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) {int _reset = 0; { + drive_channel = - drive * ica / ( 2.0 * FARADAY * depth ) ; + drive_pump = - kt * ( cai - cainf ) / ( cai + kd ) ; + if ( drive_channel <= 0. ) { + drive_channel = 0. ; + } + Dcai = drive_channel + pump * drive_pump + ( cainf - cai ) / taur ; + } + return _reset; +} + static int _ode_matsol1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + drive_channel = - drive * ica / ( 2.0 * FARADAY * depth ) ; + drive_pump = - kt * ( cai - cainf ) / ( cai + kd ) ; + if ( drive_channel <= 0. ) { + drive_channel = 0. ; + } + Dcai = Dcai / (1. - dt*( ( ( ( - 1.0 ) ) ) / taur )) ; + return 0; +} + /*END CVODE*/ + static int state (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { { + drive_channel = - drive * ica / ( 2.0 * FARADAY * depth ) ; + drive_pump = - kt * ( cai - cainf ) / ( cai + kd ) ; + if ( drive_channel <= 0. ) { + drive_channel = 0. ; + } + cai = cai + (1. - exp(dt*(( ( ( - 1.0 ) ) ) / taur)))*(- ( drive_channel + ( pump )*( drive_pump ) + ( ( cainf ) ) / taur ) / ( ( ( ( - 1.0 ) ) ) / taur ) - cai) ; + } + return 0; +} + +static int _ode_count(int _type){ return 1;} + +static void _ode_spec(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + ica = _ion_ica; + cai = _ion_cai; + cai = _ion_cai; + _ode_spec1 (_p, _ppvar, _thread, _nt); + _ion_cai = cai; + }} + +static void _ode_map(int _ieq, double** _pv, double** _pvdot, double* _pp, Datum* _ppd, double* _atol, int _type) { + double* _p; Datum* _ppvar; + int _i; _p = _pp; _ppvar = _ppd; + _cvode_ieq = _ieq; + for (_i=0; _i < 1; ++_i) { + _pv[_i] = _pp + _slist1[_i]; _pvdot[_i] = _pp + _dlist1[_i]; + _cvode_abstol(_atollist, _atol, _i); + } + _pv[0] = &(_ion_cai); + } + +static void _ode_matsol_instance1(_threadargsproto_) { + _ode_matsol1 (_p, _ppvar, _thread, _nt); + } + +static void _ode_matsol(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + ica = _ion_ica; + cai = _ion_cai; + cai = _ion_cai; + _ode_matsol_instance1(_threadargs_); + }} + extern void nrn_update_ion_pointer(Symbol*, Datum*, int, int); + static void _update_ion_pointer(Datum* _ppvar) { + nrn_update_ion_pointer(_ca_sym, _ppvar, 0, 3); + nrn_update_ion_pointer(_ca_sym, _ppvar, 1, 1); + } + +static void initmodel(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + int _i; double _save;{ + { + cai = cainf ; + } + +} +} + +static void nrn_init(NrnThread* _nt, _Memb_list* _ml, int _type){ +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v = _v; + ica = _ion_ica; + cai = _ion_cai; + cai = _ion_cai; + initmodel(_p, _ppvar, _thread, _nt); + _ion_cai = cai; + nrn_wrote_conc(_ca_sym, (&(_ion_cai)) - 1, _style_ca); +} +} + +static double _nrn_current(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, double _v){double _current=0.;v=_v;{ +} return _current; +} + +static void nrn_cur(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; double _rhs, _v; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + +} + +} + +static void nrn_jacob(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; +#if CACHEVEC + if (use_cachevec) { + VEC_D(_ni[_iml]) += _g; + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + NODED(_nd) += _g; + } + +} + +} + +static void nrn_state(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v = 0.0; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v=_v; +{ + ica = _ion_ica; + cai = _ion_cai; + cai = _ion_cai; + { state(_p, _ppvar, _thread, _nt); + } { + } + _ion_cai = cai; +}} + +} + +static void terminal(){} + +static void _initlists(){ + double _x; double* _p = &_x; + int _i; static int _first = 1; + if (!_first) return; + _slist1[0] = cai_columnindex; _dlist1[0] = Dcai_columnindex; +_first = 0; +} + +#if defined(__cplusplus) +} /* extern "C" */ +#endif + +#if NMODL_TEXT +static const char* nmodl_filename = "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/cadyn_ms.mod"; +static const char* nmodl_file_text = + "TITLE Calcium dynamics for N, P/Q, R calcium pool\n" + "\n" + "NEURON {\n" + " SUFFIX cadyn_ms\n" + " USEION ca READ ica, cai WRITE cai VALENCE 2\n" + " RANGE pump, cainf, taur, drive, depth\n" + "}\n" + "\n" + "UNITS {\n" + " (molar) = (1/liter) \n" + " (mM) = (millimolar)\n" + " (um) = (micron)\n" + " (mA) = (milliamp)\n" + " (msM) = (ms mM)\n" + " FARADAY = (faraday) (coulomb)\n" + "}\n" + "\n" + "PARAMETER {\n" + " drive = 10000 (1)\n" + " depth = 0.2 (um)\n" + " cainf = 70e-6 (mM)\n" + " taur = 43 (ms)\n" + " kt = 1e-4 (mM/ms)\n" + " kd = 1e-4 (mM)\n" + " pump = 0.02\n" + "}\n" + "\n" + "STATE { cai (mM) }\n" + "\n" + "INITIAL { cai = cainf }\n" + "\n" + "ASSIGNED {\n" + " ica (mA/cm2)\n" + " drive_channel (mM/ms)\n" + " drive_pump (mM/ms)\n" + "}\n" + " \n" + "BREAKPOINT {\n" + " SOLVE state METHOD cnexp\n" + "}\n" + "\n" + "DERIVATIVE state { \n" + " \n" + " : force concentration to stay above cainf by only pumping if larger\n" + " drive_channel = -drive*ica/(2*FARADAY*depth)\n" + " drive_pump = -kt*(cai-cainf)/(cai+kd)\n" + " \n" + " if (drive_channel <= 0.) { drive_channel = 0. }\n" + " \n" + " cai' = drive_channel + pump*drive_pump + (cainf-cai)/taur\n" + "}\n" + "\n" + "COMMENT\n" + "\n" + "Original NEURON model by Wolf (2005) and Destexhe (1992). Adaptation by\n" + "Alexander Kozlov . Updated by Robert Lindroos .\n" + "\n" + "Updates by RL:\n" + "-cainf changed from 10 to 70 nM (sabatini et al., 2002 The Life Cycle of Ca 2+ Ions in Dendritic Spines)\n" + "-pump updated to only be active if cai > cainf\n" + "\n" + "[1] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M,\n" + "O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions\n" + "and entrainment to oscillations in a computational model of the nucleus\n" + "accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95.\n" + "\n" + "ENDCOMMENT\n" + ; +#endif diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/cadyn_ms.o b/examples/example_models/str-dspn-model1/mechanisms/x86_64/cadyn_ms.o new file mode 100644 index 00000000..00f02743 Binary files /dev/null and b/examples/example_models/str-dspn-model1/mechanisms/x86_64/cadyn_ms.o differ diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/cal12_ms.c b/examples/example_models/str-dspn-model1/mechanisms/x86_64/cal12_ms.c new file mode 100644 index 00000000..455b70a6 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/x86_64/cal12_ms.c @@ -0,0 +1,814 @@ +/* Created by Language version: 7.7.0 */ +/* VECTORIZED */ +#define NRN_VECTORIZED 1 +#include +#include +#include +#include "mech_api.h" +#undef PI +#define nil 0 +#include "md1redef.h" +#include "section.h" +#include "nrniv_mf.h" +#include "md2redef.h" + +#if METHOD3 +extern int _method3; +#endif + +#if !NRNGPU +#undef exp +#define exp hoc_Exp +extern double hoc_Exp(double); +#endif + +#define nrn_init _nrn_init__cal12_ms +#define _nrn_initial _nrn_initial__cal12_ms +#define nrn_cur _nrn_cur__cal12_ms +#define _nrn_current _nrn_current__cal12_ms +#define nrn_jacob _nrn_jacob__cal12_ms +#define nrn_state _nrn_state__cal12_ms +#define _net_receive _net_receive__cal12_ms +#define rates rates__cal12_ms +#define states states__cal12_ms + +#define _threadargscomma_ _p, _ppvar, _thread, _nt, +#define _threadargsprotocomma_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, +#define _threadargs_ _p, _ppvar, _thread, _nt +#define _threadargsproto_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt + /*SUPPRESS 761*/ + /*SUPPRESS 762*/ + /*SUPPRESS 763*/ + /*SUPPRESS 765*/ + extern double *getarg(); + /* Thread safe. No static _p or _ppvar. */ + +#define t _nt->_t +#define dt _nt->_dt +#define pbar _p[0] +#define pbar_columnindex 0 +#define modDA _p[1] +#define modDA_columnindex 1 +#define maxModDA _p[2] +#define maxModDA_columnindex 2 +#define levelDA _p[3] +#define levelDA_columnindex 3 +#define modACh _p[4] +#define modACh_columnindex 4 +#define maxModACh _p[5] +#define maxModACh_columnindex 5 +#define levelACh _p[6] +#define levelACh_columnindex 6 +#define ical _p[7] +#define ical_columnindex 7 +#define m _p[8] +#define m_columnindex 8 +#define h _p[9] +#define h_columnindex 9 +#define ecal _p[10] +#define ecal_columnindex 10 +#define cali _p[11] +#define cali_columnindex 11 +#define calo _p[12] +#define calo_columnindex 12 +#define minf _p[13] +#define minf_columnindex 13 +#define mtau _p[14] +#define mtau_columnindex 14 +#define hinf _p[15] +#define hinf_columnindex 15 +#define htau _p[16] +#define htau_columnindex 16 +#define Dm _p[17] +#define Dm_columnindex 17 +#define Dh _p[18] +#define Dh_columnindex 18 +#define v _p[19] +#define v_columnindex 19 +#define _g _p[20] +#define _g_columnindex 20 +#define _ion_cali *_ppvar[0]._pval +#define _ion_calo *_ppvar[1]._pval +#define _ion_ical *_ppvar[2]._pval +#define _ion_dicaldv *_ppvar[3]._pval + +#if MAC +#if !defined(v) +#define v _mlhv +#endif +#if !defined(h) +#define h _mlhh +#endif +#endif + +#if defined(__cplusplus) +extern "C" { +#endif + static int hoc_nrnpointerindex = -1; + static Datum* _extcall_thread; + static Prop* _extcall_prop; + /* external NEURON variables */ + extern double celsius; + /* declaration of user functions */ + static void _hoc_efun(void); + static void _hoc_ghk(void); + static void _hoc_modulationACh(void); + static void _hoc_modulationDA(void); + static void _hoc_rates(void); + static int _mechtype; +extern void _nrn_cacheloop_reg(int, int); +extern void hoc_register_prop_size(int, int, int); +extern void hoc_register_limits(int, HocParmLimits*); +extern void hoc_register_units(int, HocParmUnits*); +extern void nrn_promote(Prop*, int, int); +extern Memb_func* memb_func; + +#define NMODL_TEXT 1 +#if NMODL_TEXT +static const char* nmodl_file_text; +static const char* nmodl_filename; +extern void hoc_reg_nmodl_text(int, const char*); +extern void hoc_reg_nmodl_filename(int, const char*); +#endif + + extern void _nrn_setdata_reg(int, void(*)(Prop*)); + static void _setdata(Prop* _prop) { + _extcall_prop = _prop; + } + static void _hoc_setdata() { + Prop *_prop, *hoc_getdata_range(int); + _prop = hoc_getdata_range(_mechtype); + _setdata(_prop); + hoc_retpushx(1.); +} + /* connect user functions to hoc names */ + static VoidFunc hoc_intfunc[] = { + "setdata_cal12_ms", _hoc_setdata, + "efun_cal12_ms", _hoc_efun, + "ghk_cal12_ms", _hoc_ghk, + "modulationACh_cal12_ms", _hoc_modulationACh, + "modulationDA_cal12_ms", _hoc_modulationDA, + "rates_cal12_ms", _hoc_rates, + 0, 0 +}; +#define efun efun_cal12_ms +#define ghk ghk_cal12_ms +#define modulationACh modulationACh_cal12_ms +#define modulationDA modulationDA_cal12_ms + extern double efun( _threadargsprotocomma_ double ); + extern double ghk( _threadargsprotocomma_ double , double , double ); + extern double modulationACh( _threadargsproto_ ); + extern double modulationDA( _threadargsproto_ ); + /* declare global and static user variables */ +#define a a_cal12_ms + double a = 0.17; +#define q q_cal12_ms + double q = 2; + /* some parameters have upper and lower limits */ + static HocParmLimits _hoc_parm_limits[] = { + 0,0,0 +}; + static HocParmUnits _hoc_parm_units[] = { + "pbar_cal12_ms", "cm/s", + "ical_cal12_ms", "mA/cm2", + 0,0 +}; + static double delta_t = 0.01; + static double h0 = 0; + static double m0 = 0; + /* connect global user variables to hoc */ + static DoubScal hoc_scdoub[] = { + "a_cal12_ms", &a_cal12_ms, + "q_cal12_ms", &q_cal12_ms, + 0,0 +}; + static DoubVec hoc_vdoub[] = { + 0,0,0 +}; + static double _sav_indep; + static void nrn_alloc(Prop*); +static void nrn_init(NrnThread*, _Memb_list*, int); +static void nrn_state(NrnThread*, _Memb_list*, int); + static void nrn_cur(NrnThread*, _Memb_list*, int); +static void nrn_jacob(NrnThread*, _Memb_list*, int); + +static int _ode_count(int); +static void _ode_map(int, double**, double**, double*, Datum*, double*, int); +static void _ode_spec(NrnThread*, _Memb_list*, int); +static void _ode_matsol(NrnThread*, _Memb_list*, int); + +#define _cvode_ieq _ppvar[4]._i + static void _ode_matsol_instance1(_threadargsproto_); + /* connect range variables in _p that hoc is supposed to know about */ + static const char *_mechanism[] = { + "7.7.0", +"cal12_ms", + "pbar_cal12_ms", + "modDA_cal12_ms", + "maxModDA_cal12_ms", + "levelDA_cal12_ms", + "modACh_cal12_ms", + "maxModACh_cal12_ms", + "levelACh_cal12_ms", + 0, + "ical_cal12_ms", + 0, + "m_cal12_ms", + "h_cal12_ms", + 0, + 0}; + static Symbol* _cal_sym; + +extern Prop* need_memb(Symbol*); + +static void nrn_alloc(Prop* _prop) { + Prop *prop_ion; + double *_p; Datum *_ppvar; + _p = nrn_prop_data_alloc(_mechtype, 21, _prop); + /*initialize range parameters*/ + pbar = 0; + modDA = 0; + maxModDA = 1; + levelDA = 0; + modACh = 0; + maxModACh = 1; + levelACh = 0; + _prop->param = _p; + _prop->param_size = 21; + _ppvar = nrn_prop_datum_alloc(_mechtype, 5, _prop); + _prop->dparam = _ppvar; + /*connect ionic variables to this model*/ + prop_ion = need_memb(_cal_sym); + nrn_promote(prop_ion, 1, 0); + _ppvar[0]._pval = &prop_ion->param[1]; /* cali */ + _ppvar[1]._pval = &prop_ion->param[2]; /* calo */ + _ppvar[2]._pval = &prop_ion->param[3]; /* ical */ + _ppvar[3]._pval = &prop_ion->param[4]; /* _ion_dicaldv */ + +} + static void _initlists(); + /* some states have an absolute tolerance */ + static Symbol** _atollist; + static HocStateTolerance _hoc_state_tol[] = { + 0,0 +}; + static void _update_ion_pointer(Datum*); + extern Symbol* hoc_lookup(const char*); +extern void _nrn_thread_reg(int, int, void(*)(Datum*)); +extern void _nrn_thread_table_reg(int, void(*)(double*, Datum*, Datum*, NrnThread*, int)); +extern void hoc_register_tolerance(int, HocStateTolerance*, Symbol***); +extern void _cvode_abstol( Symbol**, double*, int); + + void _cal12_ms_reg() { + int _vectorized = 1; + _initlists(); + ion_reg("cal", 2.0); + _cal_sym = hoc_lookup("cal_ion"); + register_mech(_mechanism, nrn_alloc,nrn_cur, nrn_jacob, nrn_state, nrn_init, hoc_nrnpointerindex, 1); + _mechtype = nrn_get_mechtype(_mechanism[1]); + _nrn_setdata_reg(_mechtype, _setdata); + _nrn_thread_reg(_mechtype, 2, _update_ion_pointer); + #if NMODL_TEXT + hoc_reg_nmodl_text(_mechtype, nmodl_file_text); + hoc_reg_nmodl_filename(_mechtype, nmodl_filename); +#endif + hoc_register_prop_size(_mechtype, 21, 5); + hoc_register_dparam_semantics(_mechtype, 0, "cal_ion"); + hoc_register_dparam_semantics(_mechtype, 1, "cal_ion"); + hoc_register_dparam_semantics(_mechtype, 2, "cal_ion"); + hoc_register_dparam_semantics(_mechtype, 3, "cal_ion"); + hoc_register_dparam_semantics(_mechtype, 4, "cvodeieq"); + hoc_register_cvode(_mechtype, _ode_count, _ode_map, _ode_spec, _ode_matsol); + hoc_register_tolerance(_mechtype, _hoc_state_tol, &_atollist); + hoc_register_var(hoc_scdoub, hoc_vdoub, hoc_intfunc); + ivoc_help("help ?1 cal12_ms /home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/cal12_ms.mod\n"); + hoc_register_limits(_mechtype, _hoc_parm_limits); + hoc_register_units(_mechtype, _hoc_parm_units); + } + +#define FARADAY _nrnunit_FARADAY[_nrnunit_use_legacy_] +static double _nrnunit_FARADAY[2] = {0x1.78e555060882cp+16, 96485.3}; /* 96485.3321233100141 */ + +#define R _nrnunit_R[_nrnunit_use_legacy_] +static double _nrnunit_R[2] = {0x1.0a1013e8990bep+3, 8.3145}; /* 8.3144626181532395 */ +static int _reset; +static char *modelname = "HVA L-type calcium current (Cav1.2)"; + +static int error; +static int _ninits = 0; +static int _match_recurse=1; +static void _modl_cleanup(){ _match_recurse=1;} +static int rates(_threadargsproto_); + +static int _ode_spec1(_threadargsproto_); +/*static int _ode_matsol1(_threadargsproto_);*/ + static int _slist1[2], _dlist1[2]; + static int states(_threadargsproto_); + +/*CVODE*/ + static int _ode_spec1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) {int _reset = 0; { + rates ( _threadargs_ ) ; + Dm = ( minf - m ) / mtau * q ; + Dh = ( hinf - h ) / htau * q ; + } + return _reset; +} + static int _ode_matsol1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + rates ( _threadargs_ ) ; + Dm = Dm / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) )) ; + Dh = Dh / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / htau )*( q ) )) ; + return 0; +} + /*END CVODE*/ + static int states (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { { + rates ( _threadargs_ ) ; + m = m + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / mtau )*( q ))))*(- ( ( ( ( minf ) ) / mtau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) ) - m) ; + h = h + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / htau )*( q ))))*(- ( ( ( ( hinf ) ) / htau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / htau )*( q ) ) - h) ; + } + return 0; +} + +static int rates ( _threadargsproto_ ) { + minf = 1.0 / ( 1.0 + exp ( ( v - ( - 8.9 ) ) / ( - 6.7 ) ) ) ; + mtau = 0.06 + 1.0 / ( exp ( ( v - 10.0 ) / 20.0 ) + exp ( ( v - ( - 17.0 ) ) / - 48.0 ) ) ; + hinf = 1.0 / ( 1.0 + exp ( ( v - ( - 13.4 ) ) / 11.9 ) ) ; + htau = 44.3 ; + return 0; } + +static void _hoc_rates(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = 1.; + rates ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +double ghk ( _threadargsprotocomma_ double _lv , double _lci , double _lco ) { + double _lghk; + double _lz , _leci , _leco ; + _lz = ( 1e-3 ) * 2.0 * FARADAY * _lv / ( R * ( celsius + 273.15 ) ) ; + _leco = _lco * efun ( _threadargscomma_ _lz ) ; + _leci = _lci * efun ( _threadargscomma_ - _lz ) ; + _lghk = ( 1e-3 ) * 2.0 * FARADAY * ( _leci - _leco ) ; + +return _lghk; + } + +static void _hoc_ghk(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = ghk ( _p, _ppvar, _thread, _nt, *getarg(1) , *getarg(2) , *getarg(3) ); + hoc_retpushx(_r); +} + +double efun ( _threadargsprotocomma_ double _lz ) { + double _lefun; + if ( fabs ( _lz ) < 1e-4 ) { + _lefun = 1.0 - _lz / 2.0 ; + } + else { + _lefun = _lz / ( exp ( _lz ) - 1.0 ) ; + } + +return _lefun; + } + +static void _hoc_efun(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = efun ( _p, _ppvar, _thread, _nt, *getarg(1) ); + hoc_retpushx(_r); +} + +double modulationDA ( _threadargsproto_ ) { + double _lmodulationDA; + _lmodulationDA = 1.0 + modDA * ( maxModDA - 1.0 ) * levelDA ; + +return _lmodulationDA; + } + +static void _hoc_modulationDA(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = modulationDA ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +double modulationACh ( _threadargsproto_ ) { + double _lmodulationACh; + _lmodulationACh = 1.0 + modACh * ( maxModACh - 1.0 ) * levelACh ; + +return _lmodulationACh; + } + +static void _hoc_modulationACh(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = modulationACh ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +static int _ode_count(int _type){ return 2;} + +static void _ode_spec(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + cali = _ion_cali; + calo = _ion_calo; + _ode_spec1 (_p, _ppvar, _thread, _nt); + }} + +static void _ode_map(int _ieq, double** _pv, double** _pvdot, double* _pp, Datum* _ppd, double* _atol, int _type) { + double* _p; Datum* _ppvar; + int _i; _p = _pp; _ppvar = _ppd; + _cvode_ieq = _ieq; + for (_i=0; _i < 2; ++_i) { + _pv[_i] = _pp + _slist1[_i]; _pvdot[_i] = _pp + _dlist1[_i]; + _cvode_abstol(_atollist, _atol, _i); + } + } + +static void _ode_matsol_instance1(_threadargsproto_) { + _ode_matsol1 (_p, _ppvar, _thread, _nt); + } + +static void _ode_matsol(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + cali = _ion_cali; + calo = _ion_calo; + _ode_matsol_instance1(_threadargs_); + }} + extern void nrn_update_ion_pointer(Symbol*, Datum*, int, int); + static void _update_ion_pointer(Datum* _ppvar) { + nrn_update_ion_pointer(_cal_sym, _ppvar, 0, 1); + nrn_update_ion_pointer(_cal_sym, _ppvar, 1, 2); + nrn_update_ion_pointer(_cal_sym, _ppvar, 2, 3); + nrn_update_ion_pointer(_cal_sym, _ppvar, 3, 4); + } + +static void initmodel(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + int _i; double _save;{ + h = h0; + m = m0; + { + rates ( _threadargs_ ) ; + m = minf ; + h = hinf ; + } + +} +} + +static void nrn_init(NrnThread* _nt, _Memb_list* _ml, int _type){ +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v = _v; + cali = _ion_cali; + calo = _ion_calo; + initmodel(_p, _ppvar, _thread, _nt); + } +} + +static double _nrn_current(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, double _v){double _current=0.;v=_v;{ { + ical = pbar * m * ( h * a + 1.0 - a ) * ghk ( _threadargscomma_ v , cali , calo ) * modulationDA ( _threadargs_ ) * modulationACh ( _threadargs_ ) ; + } + _current += ical; + +} return _current; +} + +static void nrn_cur(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; double _rhs, _v; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + cali = _ion_cali; + calo = _ion_calo; + _g = _nrn_current(_p, _ppvar, _thread, _nt, _v + .001); + { double _dical; + _dical = ical; + _rhs = _nrn_current(_p, _ppvar, _thread, _nt, _v); + _ion_dicaldv += (_dical - ical)/.001 ; + } + _g = (_g - _rhs)/.001; + _ion_ical += ical ; +#if CACHEVEC + if (use_cachevec) { + VEC_RHS(_ni[_iml]) -= _rhs; + }else +#endif + { + NODERHS(_nd) -= _rhs; + } + +} + +} + +static void nrn_jacob(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; +#if CACHEVEC + if (use_cachevec) { + VEC_D(_ni[_iml]) += _g; + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + NODED(_nd) += _g; + } + +} + +} + +static void nrn_state(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v = 0.0; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v=_v; +{ + cali = _ion_cali; + calo = _ion_calo; + { states(_p, _ppvar, _thread, _nt); + } }} + +} + +static void terminal(){} + +static void _initlists(){ + double _x; double* _p = &_x; + int _i; static int _first = 1; + if (!_first) return; + _slist1[0] = m_columnindex; _dlist1[0] = Dm_columnindex; + _slist1[1] = h_columnindex; _dlist1[1] = Dh_columnindex; +_first = 0; +} + +#if defined(__cplusplus) +} /* extern "C" */ +#endif + +#if NMODL_TEXT +static const char* nmodl_filename = "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/cal12_ms.mod"; +static const char* nmodl_file_text = + "TITLE HVA L-type calcium current (Cav1.2)\n" + "\n" + "COMMENT\n" + "\n" + "Neuromodulation is added as functions:\n" + " \n" + " modulationDA = 1 + modDA*(maxModDA-1)*levelDA\n" + "\n" + "where:\n" + " \n" + " modDA [0]: is a switch for turning modulation on or off {1/0}\n" + " maxModDA [1]: is the maximum modulation for this specific channel (read from the param file)\n" + " e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf}\n" + " levelDA [0]: is an additional parameter for scaling modulation. \n" + " Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1}\n" + " \n" + " Further neuromodulators can be added by for example:\n" + " modulationDA = 1 + modDA*(maxModDA-1)\n" + " modulationACh = 1 + modACh*(maxModACh-1)\n" + " ....\n" + "\n" + " etc. for other neuromodulators\n" + " \n" + " \n" + " \n" + "[] == default values\n" + "{} == ranges\n" + " \n" + "ENDCOMMENT\n" + "\n" + "\n" + "UNITS {\n" + " (mV) = (millivolt)\n" + " (mA) = (milliamp)\n" + " (S) = (siemens)\n" + " (molar) = (1/liter)\n" + " (mM) = (millimolar)\n" + " FARADAY = (faraday) (coulomb)\n" + " R = (k-mole) (joule/degC)\n" + "}\n" + "\n" + "NEURON {\n" + " SUFFIX cal12_ms\n" + " USEION cal READ cali, calo WRITE ical VALENCE 2\n" + " RANGE pbar, ical\n" + " RANGE modDA, maxModDA, levelDA\n" + " RANGE modACh, maxModACh, levelACh\n" + "}\n" + "\n" + "PARAMETER {\n" + " pbar = 0.0 (cm/s)\n" + " a = 0.17\n" + " :q = 1 : room temperature 22-25 C\n" + " q = 2 : body temperature 35 C\n" + " modDA = 0\n" + " maxModDA = 1\n" + " levelDA = 0\n" + " modACh = 0\n" + " maxModACh = 1 \n" + " levelACh = 0\n" + " \n" + "} \n" + "\n" + "ASSIGNED { \n" + " v (mV)\n" + " ical (mA/cm2)\n" + " ecal (mV)\n" + " celsius (degC)\n" + " cali (mM)\n" + " calo (mM)\n" + " minf\n" + " mtau (ms)\n" + " hinf\n" + " htau (ms)\n" + "}\n" + "\n" + "STATE { m h }\n" + "\n" + "BREAKPOINT {\n" + " SOLVE states METHOD cnexp\n" + " ical = pbar*m*(h*a+1-a)*ghk(v, cali, calo)*modulationDA()*modulationACh()\n" + "}\n" + "\n" + "INITIAL {\n" + " rates()\n" + " m = minf\n" + " h = hinf\n" + "}\n" + "\n" + "DERIVATIVE states { \n" + " rates()\n" + " m' = (minf-m)/mtau*q\n" + " h' = (hinf-h)/htau*q\n" + "}\n" + "\n" + "PROCEDURE rates() {\n" + " UNITSOFF\n" + " minf = 1/(1+exp((v-(-8.9))/(-6.7)))\n" + " mtau = 0.06+1/(exp((v-10)/20)+exp((v-(-17))/-48))\n" + " hinf = 1/(1+exp((v-(-13.4))/11.9))\n" + " htau = 44.3\n" + " UNITSON\n" + "}\n" + "\n" + "FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) {\n" + " LOCAL z, eci, eco\n" + " z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15))\n" + " eco = co*efun(z)\n" + " eci = ci*efun(-z)\n" + " ghk = (1e-3)*2*FARADAY*(eci-eco)\n" + "}\n" + "\n" + "FUNCTION efun(z) {\n" + " if (fabs(z) < 1e-4) {\n" + " efun = 1-z/2\n" + " }else{\n" + " efun = z/(exp(z)-1)\n" + " }\n" + "}\n" + "\n" + "\n" + "FUNCTION modulationDA() {\n" + " : returns modulation factor\n" + " \n" + " modulationDA = 1 + modDA*(maxModDA-1)*levelDA \n" + "}\n" + "\n" + "FUNCTION modulationACh() {\n" + " : returns modulation factor\n" + " \n" + " modulationACh = 1 + modACh*(maxModACh-1)*levelACh \n" + "}\n" + "\n" + "\n" + "COMMENT\n" + "\n" + "Activation curve was reconstructed for cultured NAc neurons from P5-P32\n" + "Charles River rat pups [1]. Activation time constant is from the\n" + "rodent neuron culture (both rat and mouse cells), room temperature 22-25\n" + "C [2, Fig.15A]. Inactivation curve of CaL v1.3 current was taken from HEK\n" + "cells [3, Fig.2 and p.819] at room temperature.\n" + "\n" + "Original NEURON model by Wolf (2005) [4] was modified by Alexander Kozlov\n" + ". Kinetics of m1h type was used [5,6]. Activation\n" + "time constant was refitted to avoid singularity.\n" + "\n" + "[1] Churchill D, Macvicar BA (1998) Biophysical and pharmacological\n" + "characterization of voltage-dependent Ca2+ channels in neurons isolated\n" + "from rat nucleus accumbens. J Neurophysiol 79(2):635-47.\n" + "\n" + "[2] Kasai H, Neher E (1992) Dihydropyridine-sensitive and\n" + "omega-conotoxin-sensitive calcium channels in a mammalian\n" + "neuroblastoma-glioma cell line. J Physiol 448:161-88.\n" + "\n" + "[3] Bell DC, Butcher AJ, Berrow NS, Page KM, Brust PF, Nesterova A,\n" + "Stauderman KA, Seabrook GR, Nurnberg B, Dolphin AC (2001) Biophysical\n" + "properties, pharmacology, and modulation of human, neuronal L-type\n" + "(alpha(1D), Ca(V)1.3) voltage-dependent calcium currents. J Neurophysiol\n" + "85:816-827.\n" + "\n" + "[4] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M,\n" + "O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions\n" + "and entrainment to oscillations in a computational model of the nucleus\n" + "accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95.\n" + "\n" + "[5] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH,\n" + "Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on\n" + "calcium influx and spike timing-dependent plasticity in striatal medium\n" + "spiny neurons. PLoS Comput Biol 8(4):e1002493.\n" + "\n" + "[6] Tuckwell HC (2012) Quantitative aspects of L-type Ca2+ currents. Prog\n" + "Neurobiol 96(1):1-31.\n" + "\n" + "add justification for modulation\n" + "\n" + "ENDCOMMENT\n" + ; +#endif diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/cal12_ms.o b/examples/example_models/str-dspn-model1/mechanisms/x86_64/cal12_ms.o new file mode 100644 index 00000000..7cf9cf9c Binary files /dev/null and b/examples/example_models/str-dspn-model1/mechanisms/x86_64/cal12_ms.o differ diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/cal13_ms.c b/examples/example_models/str-dspn-model1/mechanisms/x86_64/cal13_ms.c new file mode 100644 index 00000000..06487e90 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/x86_64/cal13_ms.c @@ -0,0 +1,813 @@ +/* Created by Language version: 7.7.0 */ +/* VECTORIZED */ +#define NRN_VECTORIZED 1 +#include +#include +#include +#include "mech_api.h" +#undef PI +#define nil 0 +#include "md1redef.h" +#include "section.h" +#include "nrniv_mf.h" +#include "md2redef.h" + +#if METHOD3 +extern int _method3; +#endif + +#if !NRNGPU +#undef exp +#define exp hoc_Exp +extern double hoc_Exp(double); +#endif + +#define nrn_init _nrn_init__cal13_ms +#define _nrn_initial _nrn_initial__cal13_ms +#define nrn_cur _nrn_cur__cal13_ms +#define _nrn_current _nrn_current__cal13_ms +#define nrn_jacob _nrn_jacob__cal13_ms +#define nrn_state _nrn_state__cal13_ms +#define _net_receive _net_receive__cal13_ms +#define rates rates__cal13_ms +#define states states__cal13_ms + +#define _threadargscomma_ _p, _ppvar, _thread, _nt, +#define _threadargsprotocomma_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, +#define _threadargs_ _p, _ppvar, _thread, _nt +#define _threadargsproto_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt + /*SUPPRESS 761*/ + /*SUPPRESS 762*/ + /*SUPPRESS 763*/ + /*SUPPRESS 765*/ + extern double *getarg(); + /* Thread safe. No static _p or _ppvar. */ + +#define t _nt->_t +#define dt _nt->_dt +#define pbar _p[0] +#define pbar_columnindex 0 +#define modDA _p[1] +#define modDA_columnindex 1 +#define maxModDA _p[2] +#define maxModDA_columnindex 2 +#define levelDA _p[3] +#define levelDA_columnindex 3 +#define modACh _p[4] +#define modACh_columnindex 4 +#define maxModACh _p[5] +#define maxModACh_columnindex 5 +#define levelACh _p[6] +#define levelACh_columnindex 6 +#define ical _p[7] +#define ical_columnindex 7 +#define m _p[8] +#define m_columnindex 8 +#define h _p[9] +#define h_columnindex 9 +#define ecal _p[10] +#define ecal_columnindex 10 +#define cali _p[11] +#define cali_columnindex 11 +#define calo _p[12] +#define calo_columnindex 12 +#define minf _p[13] +#define minf_columnindex 13 +#define mtau _p[14] +#define mtau_columnindex 14 +#define hinf _p[15] +#define hinf_columnindex 15 +#define htau _p[16] +#define htau_columnindex 16 +#define Dm _p[17] +#define Dm_columnindex 17 +#define Dh _p[18] +#define Dh_columnindex 18 +#define v _p[19] +#define v_columnindex 19 +#define _g _p[20] +#define _g_columnindex 20 +#define _ion_cali *_ppvar[0]._pval +#define _ion_calo *_ppvar[1]._pval +#define _ion_ical *_ppvar[2]._pval +#define _ion_dicaldv *_ppvar[3]._pval + +#if MAC +#if !defined(v) +#define v _mlhv +#endif +#if !defined(h) +#define h _mlhh +#endif +#endif + +#if defined(__cplusplus) +extern "C" { +#endif + static int hoc_nrnpointerindex = -1; + static Datum* _extcall_thread; + static Prop* _extcall_prop; + /* external NEURON variables */ + extern double celsius; + /* declaration of user functions */ + static void _hoc_efun(void); + static void _hoc_ghk(void); + static void _hoc_modulationACh(void); + static void _hoc_modulationDA(void); + static void _hoc_rates(void); + static int _mechtype; +extern void _nrn_cacheloop_reg(int, int); +extern void hoc_register_prop_size(int, int, int); +extern void hoc_register_limits(int, HocParmLimits*); +extern void hoc_register_units(int, HocParmUnits*); +extern void nrn_promote(Prop*, int, int); +extern Memb_func* memb_func; + +#define NMODL_TEXT 1 +#if NMODL_TEXT +static const char* nmodl_file_text; +static const char* nmodl_filename; +extern void hoc_reg_nmodl_text(int, const char*); +extern void hoc_reg_nmodl_filename(int, const char*); +#endif + + extern void _nrn_setdata_reg(int, void(*)(Prop*)); + static void _setdata(Prop* _prop) { + _extcall_prop = _prop; + } + static void _hoc_setdata() { + Prop *_prop, *hoc_getdata_range(int); + _prop = hoc_getdata_range(_mechtype); + _setdata(_prop); + hoc_retpushx(1.); +} + /* connect user functions to hoc names */ + static VoidFunc hoc_intfunc[] = { + "setdata_cal13_ms", _hoc_setdata, + "efun_cal13_ms", _hoc_efun, + "ghk_cal13_ms", _hoc_ghk, + "modulationACh_cal13_ms", _hoc_modulationACh, + "modulationDA_cal13_ms", _hoc_modulationDA, + "rates_cal13_ms", _hoc_rates, + 0, 0 +}; +#define efun efun_cal13_ms +#define ghk ghk_cal13_ms +#define modulationACh modulationACh_cal13_ms +#define modulationDA modulationDA_cal13_ms + extern double efun( _threadargsprotocomma_ double ); + extern double ghk( _threadargsprotocomma_ double , double , double ); + extern double modulationACh( _threadargsproto_ ); + extern double modulationDA( _threadargsproto_ ); + /* declare global and static user variables */ +#define q q_cal13_ms + double q = 2; + /* some parameters have upper and lower limits */ + static HocParmLimits _hoc_parm_limits[] = { + 0,0,0 +}; + static HocParmUnits _hoc_parm_units[] = { + "pbar_cal13_ms", "cm/s", + "ical_cal13_ms", "mA/cm2", + 0,0 +}; + static double delta_t = 0.01; + static double h0 = 0; + static double m0 = 0; + /* connect global user variables to hoc */ + static DoubScal hoc_scdoub[] = { + "q_cal13_ms", &q_cal13_ms, + 0,0 +}; + static DoubVec hoc_vdoub[] = { + 0,0,0 +}; + static double _sav_indep; + static void nrn_alloc(Prop*); +static void nrn_init(NrnThread*, _Memb_list*, int); +static void nrn_state(NrnThread*, _Memb_list*, int); + static void nrn_cur(NrnThread*, _Memb_list*, int); +static void nrn_jacob(NrnThread*, _Memb_list*, int); + +static int _ode_count(int); +static void _ode_map(int, double**, double**, double*, Datum*, double*, int); +static void _ode_spec(NrnThread*, _Memb_list*, int); +static void _ode_matsol(NrnThread*, _Memb_list*, int); + +#define _cvode_ieq _ppvar[4]._i + static void _ode_matsol_instance1(_threadargsproto_); + /* connect range variables in _p that hoc is supposed to know about */ + static const char *_mechanism[] = { + "7.7.0", +"cal13_ms", + "pbar_cal13_ms", + "modDA_cal13_ms", + "maxModDA_cal13_ms", + "levelDA_cal13_ms", + "modACh_cal13_ms", + "maxModACh_cal13_ms", + "levelACh_cal13_ms", + 0, + "ical_cal13_ms", + 0, + "m_cal13_ms", + "h_cal13_ms", + 0, + 0}; + static Symbol* _cal_sym; + +extern Prop* need_memb(Symbol*); + +static void nrn_alloc(Prop* _prop) { + Prop *prop_ion; + double *_p; Datum *_ppvar; + _p = nrn_prop_data_alloc(_mechtype, 21, _prop); + /*initialize range parameters*/ + pbar = 0; + modDA = 0; + maxModDA = 1; + levelDA = 0; + modACh = 0; + maxModACh = 1; + levelACh = 0; + _prop->param = _p; + _prop->param_size = 21; + _ppvar = nrn_prop_datum_alloc(_mechtype, 5, _prop); + _prop->dparam = _ppvar; + /*connect ionic variables to this model*/ + prop_ion = need_memb(_cal_sym); + nrn_promote(prop_ion, 1, 0); + _ppvar[0]._pval = &prop_ion->param[1]; /* cali */ + _ppvar[1]._pval = &prop_ion->param[2]; /* calo */ + _ppvar[2]._pval = &prop_ion->param[3]; /* ical */ + _ppvar[3]._pval = &prop_ion->param[4]; /* _ion_dicaldv */ + +} + static void _initlists(); + /* some states have an absolute tolerance */ + static Symbol** _atollist; + static HocStateTolerance _hoc_state_tol[] = { + 0,0 +}; + static void _update_ion_pointer(Datum*); + extern Symbol* hoc_lookup(const char*); +extern void _nrn_thread_reg(int, int, void(*)(Datum*)); +extern void _nrn_thread_table_reg(int, void(*)(double*, Datum*, Datum*, NrnThread*, int)); +extern void hoc_register_tolerance(int, HocStateTolerance*, Symbol***); +extern void _cvode_abstol( Symbol**, double*, int); + + void _cal13_ms_reg() { + int _vectorized = 1; + _initlists(); + ion_reg("cal", 2.0); + _cal_sym = hoc_lookup("cal_ion"); + register_mech(_mechanism, nrn_alloc,nrn_cur, nrn_jacob, nrn_state, nrn_init, hoc_nrnpointerindex, 1); + _mechtype = nrn_get_mechtype(_mechanism[1]); + _nrn_setdata_reg(_mechtype, _setdata); + _nrn_thread_reg(_mechtype, 2, _update_ion_pointer); + #if NMODL_TEXT + hoc_reg_nmodl_text(_mechtype, nmodl_file_text); + hoc_reg_nmodl_filename(_mechtype, nmodl_filename); +#endif + hoc_register_prop_size(_mechtype, 21, 5); + hoc_register_dparam_semantics(_mechtype, 0, "cal_ion"); + hoc_register_dparam_semantics(_mechtype, 1, "cal_ion"); + hoc_register_dparam_semantics(_mechtype, 2, "cal_ion"); + hoc_register_dparam_semantics(_mechtype, 3, "cal_ion"); + hoc_register_dparam_semantics(_mechtype, 4, "cvodeieq"); + hoc_register_cvode(_mechtype, _ode_count, _ode_map, _ode_spec, _ode_matsol); + hoc_register_tolerance(_mechtype, _hoc_state_tol, &_atollist); + hoc_register_var(hoc_scdoub, hoc_vdoub, hoc_intfunc); + ivoc_help("help ?1 cal13_ms /home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/cal13_ms.mod\n"); + hoc_register_limits(_mechtype, _hoc_parm_limits); + hoc_register_units(_mechtype, _hoc_parm_units); + } + +#define FARADAY _nrnunit_FARADAY[_nrnunit_use_legacy_] +static double _nrnunit_FARADAY[2] = {0x1.78e555060882cp+16, 96485.3}; /* 96485.3321233100141 */ + +#define R _nrnunit_R[_nrnunit_use_legacy_] +static double _nrnunit_R[2] = {0x1.0a1013e8990bep+3, 8.3145}; /* 8.3144626181532395 */ +static int _reset; +static char *modelname = "LVA L-type calcium current (Cav1.3)"; + +static int error; +static int _ninits = 0; +static int _match_recurse=1; +static void _modl_cleanup(){ _match_recurse=1;} +static int rates(_threadargsproto_); + +static int _ode_spec1(_threadargsproto_); +/*static int _ode_matsol1(_threadargsproto_);*/ + static int _slist1[2], _dlist1[2]; + static int states(_threadargsproto_); + +/*CVODE*/ + static int _ode_spec1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) {int _reset = 0; { + rates ( _threadargs_ ) ; + Dm = ( minf - m ) / mtau * q ; + Dh = ( hinf - h ) / htau * q ; + } + return _reset; +} + static int _ode_matsol1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + rates ( _threadargs_ ) ; + Dm = Dm / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) )) ; + Dh = Dh / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / htau )*( q ) )) ; + return 0; +} + /*END CVODE*/ + static int states (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { { + rates ( _threadargs_ ) ; + m = m + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / mtau )*( q ))))*(- ( ( ( ( minf ) ) / mtau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) ) - m) ; + h = h + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / htau )*( q ))))*(- ( ( ( ( hinf ) ) / htau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / htau )*( q ) ) - h) ; + } + return 0; +} + +static int rates ( _threadargsproto_ ) { + minf = 1.0 / ( 1.0 + exp ( ( v - ( - 33.0 ) ) / ( - 6.7 ) ) ) ; + mtau = 0.06 + 1.0 / ( exp ( ( v - 10.0 ) / 20.0 ) + exp ( ( v - ( - 17.0 ) ) / - 48.0 ) ) ; + hinf = 1.0 / ( 1.0 + exp ( ( v - ( - 13.4 ) ) / 11.9 ) ) ; + htau = 44.3 ; + return 0; } + +static void _hoc_rates(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = 1.; + rates ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +double ghk ( _threadargsprotocomma_ double _lv , double _lci , double _lco ) { + double _lghk; + double _lz , _leci , _leco ; + _lz = ( 1e-3 ) * 2.0 * FARADAY * _lv / ( R * ( celsius + 273.15 ) ) ; + _leco = _lco * efun ( _threadargscomma_ _lz ) ; + _leci = _lci * efun ( _threadargscomma_ - _lz ) ; + _lghk = ( 1e-3 ) * 2.0 * FARADAY * ( _leci - _leco ) ; + +return _lghk; + } + +static void _hoc_ghk(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = ghk ( _p, _ppvar, _thread, _nt, *getarg(1) , *getarg(2) , *getarg(3) ); + hoc_retpushx(_r); +} + +double efun ( _threadargsprotocomma_ double _lz ) { + double _lefun; + if ( fabs ( _lz ) < 1e-4 ) { + _lefun = 1.0 - _lz / 2.0 ; + } + else { + _lefun = _lz / ( exp ( _lz ) - 1.0 ) ; + } + +return _lefun; + } + +static void _hoc_efun(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = efun ( _p, _ppvar, _thread, _nt, *getarg(1) ); + hoc_retpushx(_r); +} + +double modulationDA ( _threadargsproto_ ) { + double _lmodulationDA; + _lmodulationDA = 1.0 + modDA * ( maxModDA - 1.0 ) * levelDA ; + +return _lmodulationDA; + } + +static void _hoc_modulationDA(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = modulationDA ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +double modulationACh ( _threadargsproto_ ) { + double _lmodulationACh; + _lmodulationACh = 1.0 + modACh * ( maxModACh - 1.0 ) * levelACh ; + +return _lmodulationACh; + } + +static void _hoc_modulationACh(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = modulationACh ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +static int _ode_count(int _type){ return 2;} + +static void _ode_spec(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + cali = _ion_cali; + calo = _ion_calo; + _ode_spec1 (_p, _ppvar, _thread, _nt); + }} + +static void _ode_map(int _ieq, double** _pv, double** _pvdot, double* _pp, Datum* _ppd, double* _atol, int _type) { + double* _p; Datum* _ppvar; + int _i; _p = _pp; _ppvar = _ppd; + _cvode_ieq = _ieq; + for (_i=0; _i < 2; ++_i) { + _pv[_i] = _pp + _slist1[_i]; _pvdot[_i] = _pp + _dlist1[_i]; + _cvode_abstol(_atollist, _atol, _i); + } + } + +static void _ode_matsol_instance1(_threadargsproto_) { + _ode_matsol1 (_p, _ppvar, _thread, _nt); + } + +static void _ode_matsol(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + cali = _ion_cali; + calo = _ion_calo; + _ode_matsol_instance1(_threadargs_); + }} + extern void nrn_update_ion_pointer(Symbol*, Datum*, int, int); + static void _update_ion_pointer(Datum* _ppvar) { + nrn_update_ion_pointer(_cal_sym, _ppvar, 0, 1); + nrn_update_ion_pointer(_cal_sym, _ppvar, 1, 2); + nrn_update_ion_pointer(_cal_sym, _ppvar, 2, 3); + nrn_update_ion_pointer(_cal_sym, _ppvar, 3, 4); + } + +static void initmodel(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + int _i; double _save;{ + h = h0; + m = m0; + { + rates ( _threadargs_ ) ; + m = minf ; + h = hinf ; + } + +} +} + +static void nrn_init(NrnThread* _nt, _Memb_list* _ml, int _type){ +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v = _v; + cali = _ion_cali; + calo = _ion_calo; + initmodel(_p, _ppvar, _thread, _nt); + } +} + +static double _nrn_current(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, double _v){double _current=0.;v=_v;{ { + ical = pbar * m * m * h * ghk ( _threadargscomma_ v , cali , calo ) * modulationDA ( _threadargs_ ) * modulationACh ( _threadargs_ ) ; + } + _current += ical; + +} return _current; +} + +static void nrn_cur(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; double _rhs, _v; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + cali = _ion_cali; + calo = _ion_calo; + _g = _nrn_current(_p, _ppvar, _thread, _nt, _v + .001); + { double _dical; + _dical = ical; + _rhs = _nrn_current(_p, _ppvar, _thread, _nt, _v); + _ion_dicaldv += (_dical - ical)/.001 ; + } + _g = (_g - _rhs)/.001; + _ion_ical += ical ; +#if CACHEVEC + if (use_cachevec) { + VEC_RHS(_ni[_iml]) -= _rhs; + }else +#endif + { + NODERHS(_nd) -= _rhs; + } + +} + +} + +static void nrn_jacob(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; +#if CACHEVEC + if (use_cachevec) { + VEC_D(_ni[_iml]) += _g; + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + NODED(_nd) += _g; + } + +} + +} + +static void nrn_state(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v = 0.0; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v=_v; +{ + cali = _ion_cali; + calo = _ion_calo; + { states(_p, _ppvar, _thread, _nt); + } }} + +} + +static void terminal(){} + +static void _initlists(){ + double _x; double* _p = &_x; + int _i; static int _first = 1; + if (!_first) return; + _slist1[0] = m_columnindex; _dlist1[0] = Dm_columnindex; + _slist1[1] = h_columnindex; _dlist1[1] = Dh_columnindex; +_first = 0; +} + +#if defined(__cplusplus) +} /* extern "C" */ +#endif + +#if NMODL_TEXT +static const char* nmodl_filename = "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/cal13_ms.mod"; +static const char* nmodl_file_text = + "TITLE LVA L-type calcium current (Cav1.3)\n" + "\n" + "COMMENT\n" + "\n" + "Neuromodulation is added as functions:\n" + " \n" + " modulationDA = 1 + modDA*(maxModDA-1)*levelDA\n" + "\n" + "where:\n" + " \n" + " modDA [0]: is a switch for turning modulation on or off {1/0}\n" + " maxModDA [1]: is the maximum modulation for this specific channel (read from the param file)\n" + " e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf}\n" + " levelDA [0]: is an additional parameter for scaling modulation. \n" + " Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1}\n" + " \n" + " Further neuromodulators can be added by for example:\n" + " modulationDA = 1 + modDA*(maxModDA-1)\n" + " modulationACh = 1 + modACh*(maxModACh-1)\n" + " ....\n" + "\n" + " etc. for other neuromodulators\n" + " \n" + " \n" + " \n" + "[] == default values\n" + "{} == ranges\n" + " \n" + "ENDCOMMENT\n" + "\n" + "\n" + "UNITS {\n" + " (mV) = (millivolt)\n" + " (mA) = (milliamp)\n" + " (S) = (siemens)\n" + " (molar) = (1/liter)\n" + " (mM) = (millimolar)\n" + " FARADAY = (faraday) (coulomb)\n" + " R = (k-mole) (joule/degC)\n" + "}\n" + "\n" + "NEURON {\n" + " SUFFIX cal13_ms\n" + " USEION cal READ cali, calo WRITE ical VALENCE 2\n" + " RANGE pbar, ical\n" + " RANGE modDA, maxModDA, levelDA\n" + " RANGE modACh, maxModACh, levelACh\n" + "}\n" + "\n" + "PARAMETER {\n" + " pbar = 0.0 (cm/s)\n" + " :q = 1 : room temperature 22-25 C\n" + " q = 2 : body temperature 35 C\n" + " modDA = 0\n" + " maxModDA = 1\n" + " levelDA = 0\n" + " modACh = 0\n" + " maxModACh = 1\n" + " levelACh = 0\n" + "} \n" + "\n" + "ASSIGNED { \n" + " v (mV)\n" + " ical (mA/cm2)\n" + " ecal (mV)\n" + " celsius (degC)\n" + " cali (mM)\n" + " calo (mM)\n" + " minf\n" + " mtau (ms)\n" + " hinf\n" + " htau (ms)\n" + "}\n" + "\n" + "STATE { m h }\n" + "\n" + "BREAKPOINT {\n" + " SOLVE states METHOD cnexp\n" + " ical = pbar*m*m*h*ghk(v, cali, calo)*modulationDA()*modulationACh()\n" + "}\n" + "\n" + "INITIAL {\n" + " rates()\n" + " m = minf\n" + " h = hinf\n" + "}\n" + "\n" + "DERIVATIVE states { \n" + " rates()\n" + " m' = (minf-m)/mtau*q\n" + " h' = (hinf-h)/htau*q\n" + "}\n" + "\n" + "PROCEDURE rates() {\n" + " UNITSOFF\n" + " minf = 1/(1+exp((v-(-33))/(-6.7)))\n" + " mtau = 0.06+1/(exp((v-10)/20)+exp((v-(-17))/-48))\n" + " hinf = 1/(1+exp((v-(-13.4))/11.9))\n" + " htau = 44.3\n" + " UNITSON\n" + "}\n" + "\n" + "FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) {\n" + " LOCAL z, eci, eco\n" + " z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15))\n" + " eco = co*efun(z)\n" + " eci = ci*efun(-z)\n" + " ghk = (1e-3)*2*FARADAY*(eci-eco)\n" + "}\n" + "\n" + "FUNCTION efun(z) {\n" + " if (fabs(z) < 1e-4) {\n" + " efun = 1-z/2\n" + " }else{\n" + " efun = z/(exp(z)-1)\n" + " }\n" + "}\n" + "\n" + "\n" + "FUNCTION modulationDA() {\n" + " : returns modulation factor\n" + " \n" + " modulationDA = 1 + modDA*(maxModDA-1)*levelDA \n" + "}\n" + "\n" + "FUNCTION modulationACh() {\n" + " : returns modulation factor\n" + " \n" + " modulationACh = 1 + modACh*(maxModACh-1)*levelACh \n" + "}\n" + "\n" + "\n" + "COMMENT\n" + "\n" + "Activation curve was reconstructed for cultured NAc neurons from\n" + "P5-P32 Charles River rat pups [1] and shifted to match LVA data [7,\n" + "Fig.1D]. Activation time constant is from the rodent neuron culture (both\n" + "rat and mouse cells), room temperature 22-25 C [2, Fig.15A]. Inactivation\n" + "curve of CaL v1.3 current was taken from HEK cells [3, Fig.2 and p.819]\n" + "at room temperature.\n" + "\n" + "Original NEURON model by Wolf (2005) [4] was modified by Alexander Kozlov\n" + ". Kinetics of m2h type was used [5,6]. Activation\n" + "time constant was refitted to avoid singularity.\n" + "\n" + "[1] Churchill D, Macvicar BA (1998) Biophysical and pharmacological\n" + "characterization of voltage-dependent Ca2+ channels in neurons isolated\n" + "from rat nucleus accumbens. J Neurophysiol 79(2):635-47.\n" + "\n" + "[2] Kasai H, Neher E (1992) Dihydropyridine-sensitive and\n" + "omega-conotoxin-sensitive calcium channels in a mammalian\n" + "neuroblastoma-glioma cell line. J Physiol 448:161-88.\n" + "\n" + "[3] Bell DC, Butcher AJ, Berrow NS, Page KM, Brust PF, Nesterova A,\n" + "Stauderman KA, Seabrook GR, Nurnberg B, Dolphin AC (2001) Biophysical\n" + "properties, pharmacology, and modulation of human, neuronal L-type\n" + "(alpha(1D), Ca(V)1.3) voltage-dependent calcium currents. J Neurophysiol\n" + "85:816-827.\n" + "\n" + "[4] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M,\n" + "O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions\n" + "and entrainment to oscillations in a computational model of the nucleus\n" + "accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95.\n" + "\n" + "[5] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH,\n" + "Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on\n" + "calcium influx and spike timing-dependent plasticity in striatal medium\n" + "spiny neurons. PLoS Comput Biol 8(4):e1002493.\n" + "\n" + "[6] Tuckwell HC (2012) Quantitative aspects of L-type Ca2+ currents. Prog\n" + "Neurobiol 96(1):1-31.\n" + "\n" + "[7] Xu W, Lipscombe D (2001) Neuronal cav1.3 L-type channels activate\n" + "at relatively hyperpolarized membrane potentials and are incompletely\n" + "inhibited by dihydropyridines. J Neurosci 21(16): 5944-5951.\n" + "\n" + "\n" + "ENDCOMMENT\n" + ; +#endif diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/cal13_ms.o b/examples/example_models/str-dspn-model1/mechanisms/x86_64/cal13_ms.o new file mode 100644 index 00000000..1c2f2c08 Binary files /dev/null and b/examples/example_models/str-dspn-model1/mechanisms/x86_64/cal13_ms.o differ diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/caldyn_ms.c b/examples/example_models/str-dspn-model1/mechanisms/x86_64/caldyn_ms.c new file mode 100644 index 00000000..c84dfb68 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/x86_64/caldyn_ms.c @@ -0,0 +1,555 @@ +/* Created by Language version: 7.7.0 */ +/* VECTORIZED */ +#define NRN_VECTORIZED 1 +#include +#include +#include +#include "mech_api.h" +#undef PI +#define nil 0 +#include "md1redef.h" +#include "section.h" +#include "nrniv_mf.h" +#include "md2redef.h" + +#if METHOD3 +extern int _method3; +#endif + +#if !NRNGPU +#undef exp +#define exp hoc_Exp +extern double hoc_Exp(double); +#endif + +#define nrn_init _nrn_init__caldyn_ms +#define _nrn_initial _nrn_initial__caldyn_ms +#define nrn_cur _nrn_cur__caldyn_ms +#define _nrn_current _nrn_current__caldyn_ms +#define nrn_jacob _nrn_jacob__caldyn_ms +#define nrn_state _nrn_state__caldyn_ms +#define _net_receive _net_receive__caldyn_ms +#define state state__caldyn_ms + +#define _threadargscomma_ _p, _ppvar, _thread, _nt, +#define _threadargsprotocomma_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, +#define _threadargs_ _p, _ppvar, _thread, _nt +#define _threadargsproto_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt + /*SUPPRESS 761*/ + /*SUPPRESS 762*/ + /*SUPPRESS 763*/ + /*SUPPRESS 765*/ + extern double *getarg(); + /* Thread safe. No static _p or _ppvar. */ + +#define t _nt->_t +#define dt _nt->_dt +#define drive _p[0] +#define drive_columnindex 0 +#define depth _p[1] +#define depth_columnindex 1 +#define cainf _p[2] +#define cainf_columnindex 2 +#define taur _p[3] +#define taur_columnindex 3 +#define pump _p[4] +#define pump_columnindex 4 +#define cali _p[5] +#define cali_columnindex 5 +#define Dcali _p[6] +#define Dcali_columnindex 6 +#define ical _p[7] +#define ical_columnindex 7 +#define drive_channel _p[8] +#define drive_channel_columnindex 8 +#define drive_pump _p[9] +#define drive_pump_columnindex 9 +#define v _p[10] +#define v_columnindex 10 +#define _g _p[11] +#define _g_columnindex 11 +#define _ion_ical *_ppvar[0]._pval +#define _ion_cali *_ppvar[1]._pval +#define _style_cal *((int*)_ppvar[2]._pvoid) + +#if MAC +#if !defined(v) +#define v _mlhv +#endif +#if !defined(h) +#define h _mlhh +#endif +#endif + +#if defined(__cplusplus) +extern "C" { +#endif + static int hoc_nrnpointerindex = -1; + static Datum* _extcall_thread; + static Prop* _extcall_prop; + /* external NEURON variables */ + /* declaration of user functions */ + static int _mechtype; +extern void _nrn_cacheloop_reg(int, int); +extern void hoc_register_prop_size(int, int, int); +extern void hoc_register_limits(int, HocParmLimits*); +extern void hoc_register_units(int, HocParmUnits*); +extern void nrn_promote(Prop*, int, int); +extern Memb_func* memb_func; + +#define NMODL_TEXT 1 +#if NMODL_TEXT +static const char* nmodl_file_text; +static const char* nmodl_filename; +extern void hoc_reg_nmodl_text(int, const char*); +extern void hoc_reg_nmodl_filename(int, const char*); +#endif + + extern void _nrn_setdata_reg(int, void(*)(Prop*)); + static void _setdata(Prop* _prop) { + _extcall_prop = _prop; + } + static void _hoc_setdata() { + Prop *_prop, *hoc_getdata_range(int); + _prop = hoc_getdata_range(_mechtype); + _setdata(_prop); + hoc_retpushx(1.); +} + /* connect user functions to hoc names */ + static VoidFunc hoc_intfunc[] = { + "setdata_caldyn_ms", _hoc_setdata, + 0, 0 +}; + /* declare global and static user variables */ +#define kd kd_caldyn_ms + double kd = 0.0001; +#define kt kt_caldyn_ms + double kt = 0.0001; + /* some parameters have upper and lower limits */ + static HocParmLimits _hoc_parm_limits[] = { + 0,0,0 +}; + static HocParmUnits _hoc_parm_units[] = { + "kt_caldyn_ms", "mM/ms", + "kd_caldyn_ms", "mM", + "drive_caldyn_ms", "1", + "depth_caldyn_ms", "um", + "cainf_caldyn_ms", "mM", + "taur_caldyn_ms", "ms", + 0,0 +}; + static double cali0 = 0; + static double delta_t = 0.01; + /* connect global user variables to hoc */ + static DoubScal hoc_scdoub[] = { + "kt_caldyn_ms", &kt_caldyn_ms, + "kd_caldyn_ms", &kd_caldyn_ms, + 0,0 +}; + static DoubVec hoc_vdoub[] = { + 0,0,0 +}; + static double _sav_indep; + static void nrn_alloc(Prop*); +static void nrn_init(NrnThread*, _Memb_list*, int); +static void nrn_state(NrnThread*, _Memb_list*, int); + static void nrn_cur(NrnThread*, _Memb_list*, int); +static void nrn_jacob(NrnThread*, _Memb_list*, int); + +static int _ode_count(int); +static void _ode_map(int, double**, double**, double*, Datum*, double*, int); +static void _ode_spec(NrnThread*, _Memb_list*, int); +static void _ode_matsol(NrnThread*, _Memb_list*, int); + +#define _cvode_ieq _ppvar[3]._i + static void _ode_matsol_instance1(_threadargsproto_); + /* connect range variables in _p that hoc is supposed to know about */ + static const char *_mechanism[] = { + "7.7.0", +"caldyn_ms", + "drive_caldyn_ms", + "depth_caldyn_ms", + "cainf_caldyn_ms", + "taur_caldyn_ms", + "pump_caldyn_ms", + 0, + 0, + 0, + 0}; + static Symbol* _cal_sym; + +extern Prop* need_memb(Symbol*); + +static void nrn_alloc(Prop* _prop) { + Prop *prop_ion; + double *_p; Datum *_ppvar; + _p = nrn_prop_data_alloc(_mechtype, 12, _prop); + /*initialize range parameters*/ + drive = 10000; + depth = 0.2; + cainf = 7e-05; + taur = 43; + pump = 0.02; + _prop->param = _p; + _prop->param_size = 12; + _ppvar = nrn_prop_datum_alloc(_mechtype, 4, _prop); + _prop->dparam = _ppvar; + /*connect ionic variables to this model*/ + prop_ion = need_memb(_cal_sym); + nrn_check_conc_write(_prop, prop_ion, 1); + nrn_promote(prop_ion, 3, 0); + _ppvar[0]._pval = &prop_ion->param[3]; /* ical */ + _ppvar[1]._pval = &prop_ion->param[1]; /* cali */ + _ppvar[2]._pvoid = (void*)(&(prop_ion->dparam[0]._i)); /* iontype for cal */ + +} + static void _initlists(); + /* some states have an absolute tolerance */ + static Symbol** _atollist; + static HocStateTolerance _hoc_state_tol[] = { + 0,0 +}; + static void _update_ion_pointer(Datum*); + extern Symbol* hoc_lookup(const char*); +extern void _nrn_thread_reg(int, int, void(*)(Datum*)); +extern void _nrn_thread_table_reg(int, void(*)(double*, Datum*, Datum*, NrnThread*, int)); +extern void hoc_register_tolerance(int, HocStateTolerance*, Symbol***); +extern void _cvode_abstol( Symbol**, double*, int); + + void _caldyn_ms_reg() { + int _vectorized = 1; + _initlists(); + ion_reg("cal", 2.0); + _cal_sym = hoc_lookup("cal_ion"); + register_mech(_mechanism, nrn_alloc,nrn_cur, nrn_jacob, nrn_state, nrn_init, hoc_nrnpointerindex, 1); + _mechtype = nrn_get_mechtype(_mechanism[1]); + _nrn_setdata_reg(_mechtype, _setdata); + _nrn_thread_reg(_mechtype, 2, _update_ion_pointer); + #if NMODL_TEXT + hoc_reg_nmodl_text(_mechtype, nmodl_file_text); + hoc_reg_nmodl_filename(_mechtype, nmodl_filename); +#endif + hoc_register_prop_size(_mechtype, 12, 4); + hoc_register_dparam_semantics(_mechtype, 0, "cal_ion"); + hoc_register_dparam_semantics(_mechtype, 1, "cal_ion"); + hoc_register_dparam_semantics(_mechtype, 2, "#cal_ion"); + hoc_register_dparam_semantics(_mechtype, 3, "cvodeieq"); + nrn_writes_conc(_mechtype, 0); + hoc_register_cvode(_mechtype, _ode_count, _ode_map, _ode_spec, _ode_matsol); + hoc_register_tolerance(_mechtype, _hoc_state_tol, &_atollist); + hoc_register_var(hoc_scdoub, hoc_vdoub, hoc_intfunc); + ivoc_help("help ?1 caldyn_ms /home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/caldyn_ms.mod\n"); + hoc_register_limits(_mechtype, _hoc_parm_limits); + hoc_register_units(_mechtype, _hoc_parm_units); + } + +#define FARADAY _nrnunit_FARADAY[_nrnunit_use_legacy_] +static double _nrnunit_FARADAY[2] = {0x1.78e555060882cp+16, 96485.3}; /* 96485.3321233100141 */ +static int _reset; +static char *modelname = "Calcium dynamics for L and T calcium pool"; + +static int error; +static int _ninits = 0; +static int _match_recurse=1; +static void _modl_cleanup(){ _match_recurse=1;} + +static int _ode_spec1(_threadargsproto_); +/*static int _ode_matsol1(_threadargsproto_);*/ + static int _slist1[1], _dlist1[1]; + static int state(_threadargsproto_); + +/*CVODE*/ + static int _ode_spec1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) {int _reset = 0; { + drive_channel = - drive * ical / ( 2.0 * FARADAY * depth ) ; + drive_pump = - kt * ( cali - cainf ) / ( cali + kd ) ; + if ( drive_channel <= 0. ) { + drive_channel = 0. ; + } + Dcali = drive_channel + pump * drive_pump + ( cainf - cali ) / taur ; + } + return _reset; +} + static int _ode_matsol1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + drive_channel = - drive * ical / ( 2.0 * FARADAY * depth ) ; + drive_pump = - kt * ( cali - cainf ) / ( cali + kd ) ; + if ( drive_channel <= 0. ) { + drive_channel = 0. ; + } + Dcali = Dcali / (1. - dt*( ( ( ( - 1.0 ) ) ) / taur )) ; + return 0; +} + /*END CVODE*/ + static int state (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { { + drive_channel = - drive * ical / ( 2.0 * FARADAY * depth ) ; + drive_pump = - kt * ( cali - cainf ) / ( cali + kd ) ; + if ( drive_channel <= 0. ) { + drive_channel = 0. ; + } + cali = cali + (1. - exp(dt*(( ( ( - 1.0 ) ) ) / taur)))*(- ( drive_channel + ( pump )*( drive_pump ) + ( ( cainf ) ) / taur ) / ( ( ( ( - 1.0 ) ) ) / taur ) - cali) ; + } + return 0; +} + +static int _ode_count(int _type){ return 1;} + +static void _ode_spec(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + ical = _ion_ical; + cali = _ion_cali; + cali = _ion_cali; + _ode_spec1 (_p, _ppvar, _thread, _nt); + _ion_cali = cali; + }} + +static void _ode_map(int _ieq, double** _pv, double** _pvdot, double* _pp, Datum* _ppd, double* _atol, int _type) { + double* _p; Datum* _ppvar; + int _i; _p = _pp; _ppvar = _ppd; + _cvode_ieq = _ieq; + for (_i=0; _i < 1; ++_i) { + _pv[_i] = _pp + _slist1[_i]; _pvdot[_i] = _pp + _dlist1[_i]; + _cvode_abstol(_atollist, _atol, _i); + } + _pv[0] = &(_ion_cali); + } + +static void _ode_matsol_instance1(_threadargsproto_) { + _ode_matsol1 (_p, _ppvar, _thread, _nt); + } + +static void _ode_matsol(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + ical = _ion_ical; + cali = _ion_cali; + cali = _ion_cali; + _ode_matsol_instance1(_threadargs_); + }} + extern void nrn_update_ion_pointer(Symbol*, Datum*, int, int); + static void _update_ion_pointer(Datum* _ppvar) { + nrn_update_ion_pointer(_cal_sym, _ppvar, 0, 3); + nrn_update_ion_pointer(_cal_sym, _ppvar, 1, 1); + } + +static void initmodel(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + int _i; double _save;{ + { + cali = cainf ; + } + +} +} + +static void nrn_init(NrnThread* _nt, _Memb_list* _ml, int _type){ +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v = _v; + ical = _ion_ical; + cali = _ion_cali; + cali = _ion_cali; + initmodel(_p, _ppvar, _thread, _nt); + _ion_cali = cali; + nrn_wrote_conc(_cal_sym, (&(_ion_cali)) - 1, _style_cal); +} +} + +static double _nrn_current(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, double _v){double _current=0.;v=_v;{ +} return _current; +} + +static void nrn_cur(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; double _rhs, _v; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + +} + +} + +static void nrn_jacob(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; +#if CACHEVEC + if (use_cachevec) { + VEC_D(_ni[_iml]) += _g; + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + NODED(_nd) += _g; + } + +} + +} + +static void nrn_state(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v = 0.0; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v=_v; +{ + ical = _ion_ical; + cali = _ion_cali; + cali = _ion_cali; + { state(_p, _ppvar, _thread, _nt); + } { + } + _ion_cali = cali; +}} + +} + +static void terminal(){} + +static void _initlists(){ + double _x; double* _p = &_x; + int _i; static int _first = 1; + if (!_first) return; + _slist1[0] = cali_columnindex; _dlist1[0] = Dcali_columnindex; +_first = 0; +} + +#if defined(__cplusplus) +} /* extern "C" */ +#endif + +#if NMODL_TEXT +static const char* nmodl_filename = "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/caldyn_ms.mod"; +static const char* nmodl_file_text = + "TITLE Calcium dynamics for L and T calcium pool\n" + "\n" + "NEURON {\n" + " SUFFIX caldyn_ms\n" + " USEION cal READ ical, cali WRITE cali VALENCE 2\n" + " RANGE pump, cainf, taur, drive, depth\n" + "}\n" + "\n" + "UNITS {\n" + " (molar) = (1/liter) \n" + " (mM) = (millimolar)\n" + " (um) = (micron)\n" + " (mA) = (milliamp)\n" + " (msM) = (ms mM)\n" + " FARADAY = (faraday) (coulomb)\n" + "}\n" + "\n" + "PARAMETER {\n" + " drive = 10000 (1)\n" + " depth = 0.2 (um)\n" + " cainf = 70e-6 (mM)\n" + " taur = 43 (ms)\n" + " kt = 1e-4 (mM/ms)\n" + " kd = 1e-4 (mM)\n" + " pump = 0.02\n" + "}\n" + "\n" + "STATE { cali (mM) }\n" + "\n" + "INITIAL { cali = cainf }\n" + "\n" + "ASSIGNED {\n" + " ical (mA/cm2)\n" + " drive_channel (mM/ms)\n" + " drive_pump (mM/ms)\n" + "}\n" + " \n" + "BREAKPOINT {\n" + " SOLVE state METHOD cnexp\n" + "}\n" + "\n" + "\n" + "DERIVATIVE state { \n" + " \n" + " : force concentration to stay above cainf by only pumping if larger\n" + " drive_channel = -drive*ical/(2*FARADAY*depth)\n" + " drive_pump = -kt*(cali-cainf)/(cali+kd)\n" + " \n" + " if (drive_channel <= 0.) { drive_channel = 0. }\n" + " \n" + " cali' = drive_channel + pump*drive_pump + (cainf-cali)/taur\n" + "}\n" + "\n" + "COMMENT\n" + "\n" + "Original NEURON model by Wolf (2005) and Destexhe (1992). Adaptation by\n" + "Alexander Kozlov . Updated by Robert Lindroos .\n" + "\n" + "Updates by RL:\n" + "-cainf changed from 10 to 70 nM (sabatini et al., 2002 The Life Cycle of Ca 2+ Ions in Dendritic Spines)\n" + "-pump updated to only be active if cai > cainf (neutralized by adding reversed entity) \n" + "\n" + "[1] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M,\n" + "O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions\n" + "and entrainment to oscillations in a computational model of the nucleus\n" + "accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95.\n" + "\n" + "ENDCOMMENT\n" + ; +#endif diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/caldyn_ms.o b/examples/example_models/str-dspn-model1/mechanisms/x86_64/caldyn_ms.o new file mode 100644 index 00000000..e2d1b6ec Binary files /dev/null and b/examples/example_models/str-dspn-model1/mechanisms/x86_64/caldyn_ms.o differ diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/can_ms.c b/examples/example_models/str-dspn-model1/mechanisms/x86_64/can_ms.c new file mode 100644 index 00000000..042022ca --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/x86_64/can_ms.c @@ -0,0 +1,808 @@ +/* Created by Language version: 7.7.0 */ +/* VECTORIZED */ +#define NRN_VECTORIZED 1 +#include +#include +#include +#include "mech_api.h" +#undef PI +#define nil 0 +#include "md1redef.h" +#include "section.h" +#include "nrniv_mf.h" +#include "md2redef.h" + +#if METHOD3 +extern int _method3; +#endif + +#if !NRNGPU +#undef exp +#define exp hoc_Exp +extern double hoc_Exp(double); +#endif + +#define nrn_init _nrn_init__can_ms +#define _nrn_initial _nrn_initial__can_ms +#define nrn_cur _nrn_cur__can_ms +#define _nrn_current _nrn_current__can_ms +#define nrn_jacob _nrn_jacob__can_ms +#define nrn_state _nrn_state__can_ms +#define _net_receive _net_receive__can_ms +#define rates rates__can_ms +#define states states__can_ms + +#define _threadargscomma_ _p, _ppvar, _thread, _nt, +#define _threadargsprotocomma_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, +#define _threadargs_ _p, _ppvar, _thread, _nt +#define _threadargsproto_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt + /*SUPPRESS 761*/ + /*SUPPRESS 762*/ + /*SUPPRESS 763*/ + /*SUPPRESS 765*/ + extern double *getarg(); + /* Thread safe. No static _p or _ppvar. */ + +#define t _nt->_t +#define dt _nt->_dt +#define pbar _p[0] +#define pbar_columnindex 0 +#define modDA _p[1] +#define modDA_columnindex 1 +#define maxModDA _p[2] +#define maxModDA_columnindex 2 +#define levelDA _p[3] +#define levelDA_columnindex 3 +#define modACh _p[4] +#define modACh_columnindex 4 +#define maxModACh _p[5] +#define maxModACh_columnindex 5 +#define levelACh _p[6] +#define levelACh_columnindex 6 +#define ica _p[7] +#define ica_columnindex 7 +#define m _p[8] +#define m_columnindex 8 +#define h _p[9] +#define h_columnindex 9 +#define eca _p[10] +#define eca_columnindex 10 +#define cai _p[11] +#define cai_columnindex 11 +#define cao _p[12] +#define cao_columnindex 12 +#define minf _p[13] +#define minf_columnindex 13 +#define mtau _p[14] +#define mtau_columnindex 14 +#define hinf _p[15] +#define hinf_columnindex 15 +#define htau _p[16] +#define htau_columnindex 16 +#define Dm _p[17] +#define Dm_columnindex 17 +#define Dh _p[18] +#define Dh_columnindex 18 +#define v _p[19] +#define v_columnindex 19 +#define _g _p[20] +#define _g_columnindex 20 +#define _ion_cai *_ppvar[0]._pval +#define _ion_cao *_ppvar[1]._pval +#define _ion_ica *_ppvar[2]._pval +#define _ion_dicadv *_ppvar[3]._pval + +#if MAC +#if !defined(v) +#define v _mlhv +#endif +#if !defined(h) +#define h _mlhh +#endif +#endif + +#if defined(__cplusplus) +extern "C" { +#endif + static int hoc_nrnpointerindex = -1; + static Datum* _extcall_thread; + static Prop* _extcall_prop; + /* external NEURON variables */ + extern double celsius; + /* declaration of user functions */ + static void _hoc_efun(void); + static void _hoc_ghk(void); + static void _hoc_modulationACh(void); + static void _hoc_modulationDA(void); + static void _hoc_rates(void); + static int _mechtype; +extern void _nrn_cacheloop_reg(int, int); +extern void hoc_register_prop_size(int, int, int); +extern void hoc_register_limits(int, HocParmLimits*); +extern void hoc_register_units(int, HocParmUnits*); +extern void nrn_promote(Prop*, int, int); +extern Memb_func* memb_func; + +#define NMODL_TEXT 1 +#if NMODL_TEXT +static const char* nmodl_file_text; +static const char* nmodl_filename; +extern void hoc_reg_nmodl_text(int, const char*); +extern void hoc_reg_nmodl_filename(int, const char*); +#endif + + extern void _nrn_setdata_reg(int, void(*)(Prop*)); + static void _setdata(Prop* _prop) { + _extcall_prop = _prop; + } + static void _hoc_setdata() { + Prop *_prop, *hoc_getdata_range(int); + _prop = hoc_getdata_range(_mechtype); + _setdata(_prop); + hoc_retpushx(1.); +} + /* connect user functions to hoc names */ + static VoidFunc hoc_intfunc[] = { + "setdata_can_ms", _hoc_setdata, + "efun_can_ms", _hoc_efun, + "ghk_can_ms", _hoc_ghk, + "modulationACh_can_ms", _hoc_modulationACh, + "modulationDA_can_ms", _hoc_modulationDA, + "rates_can_ms", _hoc_rates, + 0, 0 +}; +#define efun efun_can_ms +#define ghk ghk_can_ms +#define modulationACh modulationACh_can_ms +#define modulationDA modulationDA_can_ms + extern double efun( _threadargsprotocomma_ double ); + extern double ghk( _threadargsprotocomma_ double , double , double ); + extern double modulationACh( _threadargsproto_ ); + extern double modulationDA( _threadargsproto_ ); + /* declare global and static user variables */ +#define a a_can_ms + double a = 0.21; +#define q q_can_ms + double q = 3; + /* some parameters have upper and lower limits */ + static HocParmLimits _hoc_parm_limits[] = { + 0,0,0 +}; + static HocParmUnits _hoc_parm_units[] = { + "pbar_can_ms", "cm/s", + "ica_can_ms", "mA/cm2", + 0,0 +}; + static double delta_t = 0.01; + static double h0 = 0; + static double m0 = 0; + /* connect global user variables to hoc */ + static DoubScal hoc_scdoub[] = { + "a_can_ms", &a_can_ms, + "q_can_ms", &q_can_ms, + 0,0 +}; + static DoubVec hoc_vdoub[] = { + 0,0,0 +}; + static double _sav_indep; + static void nrn_alloc(Prop*); +static void nrn_init(NrnThread*, _Memb_list*, int); +static void nrn_state(NrnThread*, _Memb_list*, int); + static void nrn_cur(NrnThread*, _Memb_list*, int); +static void nrn_jacob(NrnThread*, _Memb_list*, int); + +static int _ode_count(int); +static void _ode_map(int, double**, double**, double*, Datum*, double*, int); +static void _ode_spec(NrnThread*, _Memb_list*, int); +static void _ode_matsol(NrnThread*, _Memb_list*, int); + +#define _cvode_ieq _ppvar[4]._i + static void _ode_matsol_instance1(_threadargsproto_); + /* connect range variables in _p that hoc is supposed to know about */ + static const char *_mechanism[] = { + "7.7.0", +"can_ms", + "pbar_can_ms", + "modDA_can_ms", + "maxModDA_can_ms", + "levelDA_can_ms", + "modACh_can_ms", + "maxModACh_can_ms", + "levelACh_can_ms", + 0, + "ica_can_ms", + 0, + "m_can_ms", + "h_can_ms", + 0, + 0}; + static Symbol* _ca_sym; + +extern Prop* need_memb(Symbol*); + +static void nrn_alloc(Prop* _prop) { + Prop *prop_ion; + double *_p; Datum *_ppvar; + _p = nrn_prop_data_alloc(_mechtype, 21, _prop); + /*initialize range parameters*/ + pbar = 0; + modDA = 0; + maxModDA = 1; + levelDA = 0; + modACh = 0; + maxModACh = 1; + levelACh = 0; + _prop->param = _p; + _prop->param_size = 21; + _ppvar = nrn_prop_datum_alloc(_mechtype, 5, _prop); + _prop->dparam = _ppvar; + /*connect ionic variables to this model*/ + prop_ion = need_memb(_ca_sym); + nrn_promote(prop_ion, 1, 0); + _ppvar[0]._pval = &prop_ion->param[1]; /* cai */ + _ppvar[1]._pval = &prop_ion->param[2]; /* cao */ + _ppvar[2]._pval = &prop_ion->param[3]; /* ica */ + _ppvar[3]._pval = &prop_ion->param[4]; /* _ion_dicadv */ + +} + static void _initlists(); + /* some states have an absolute tolerance */ + static Symbol** _atollist; + static HocStateTolerance _hoc_state_tol[] = { + 0,0 +}; + static void _update_ion_pointer(Datum*); + extern Symbol* hoc_lookup(const char*); +extern void _nrn_thread_reg(int, int, void(*)(Datum*)); +extern void _nrn_thread_table_reg(int, void(*)(double*, Datum*, Datum*, NrnThread*, int)); +extern void hoc_register_tolerance(int, HocStateTolerance*, Symbol***); +extern void _cvode_abstol( Symbol**, double*, int); + + void _can_ms_reg() { + int _vectorized = 1; + _initlists(); + ion_reg("ca", 2.0); + _ca_sym = hoc_lookup("ca_ion"); + register_mech(_mechanism, nrn_alloc,nrn_cur, nrn_jacob, nrn_state, nrn_init, hoc_nrnpointerindex, 1); + _mechtype = nrn_get_mechtype(_mechanism[1]); + _nrn_setdata_reg(_mechtype, _setdata); + _nrn_thread_reg(_mechtype, 2, _update_ion_pointer); + #if NMODL_TEXT + hoc_reg_nmodl_text(_mechtype, nmodl_file_text); + hoc_reg_nmodl_filename(_mechtype, nmodl_filename); +#endif + hoc_register_prop_size(_mechtype, 21, 5); + hoc_register_dparam_semantics(_mechtype, 0, "ca_ion"); + hoc_register_dparam_semantics(_mechtype, 1, "ca_ion"); + hoc_register_dparam_semantics(_mechtype, 2, "ca_ion"); + hoc_register_dparam_semantics(_mechtype, 3, "ca_ion"); + hoc_register_dparam_semantics(_mechtype, 4, "cvodeieq"); + hoc_register_cvode(_mechtype, _ode_count, _ode_map, _ode_spec, _ode_matsol); + hoc_register_tolerance(_mechtype, _hoc_state_tol, &_atollist); + hoc_register_var(hoc_scdoub, hoc_vdoub, hoc_intfunc); + ivoc_help("help ?1 can_ms /home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/can_ms.mod\n"); + hoc_register_limits(_mechtype, _hoc_parm_limits); + hoc_register_units(_mechtype, _hoc_parm_units); + } + +#define FARADAY _nrnunit_FARADAY[_nrnunit_use_legacy_] +static double _nrnunit_FARADAY[2] = {0x1.78e555060882cp+16, 96485.3}; /* 96485.3321233100141 */ + +#define R _nrnunit_R[_nrnunit_use_legacy_] +static double _nrnunit_R[2] = {0x1.0a1013e8990bep+3, 8.3145}; /* 8.3144626181532395 */ +static int _reset; +static char *modelname = "N-type calcium current (Cav2.2)"; + +static int error; +static int _ninits = 0; +static int _match_recurse=1; +static void _modl_cleanup(){ _match_recurse=1;} +static int rates(_threadargsproto_); + +static int _ode_spec1(_threadargsproto_); +/*static int _ode_matsol1(_threadargsproto_);*/ + static int _slist1[2], _dlist1[2]; + static int states(_threadargsproto_); + +/*CVODE*/ + static int _ode_spec1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) {int _reset = 0; { + rates ( _threadargs_ ) ; + Dm = ( minf - m ) / mtau * q ; + Dh = ( hinf - h ) / htau * q ; + } + return _reset; +} + static int _ode_matsol1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + rates ( _threadargs_ ) ; + Dm = Dm / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) )) ; + Dh = Dh / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / htau )*( q ) )) ; + return 0; +} + /*END CVODE*/ + static int states (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { { + rates ( _threadargs_ ) ; + m = m + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / mtau )*( q ))))*(- ( ( ( ( minf ) ) / mtau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) ) - m) ; + h = h + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / htau )*( q ))))*(- ( ( ( ( hinf ) ) / htau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / htau )*( q ) ) - h) ; + } + return 0; +} + +static int rates ( _threadargsproto_ ) { + minf = 1.0 / ( 1.0 + exp ( ( v - ( - 3.0 ) ) / ( - 8.0 ) ) ) ; + mtau = 0.06 + 1.0 / ( exp ( ( v - 25.0 ) / 18.0 ) + exp ( ( v - ( - 31.0 ) ) / ( - 44.0 ) ) ) ; + hinf = 1.0 / ( 1.0 + exp ( ( v - ( - 74.8 ) ) / 6.5 ) ) ; + htau = 70.0 ; + return 0; } + +static void _hoc_rates(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = 1.; + rates ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +double ghk ( _threadargsprotocomma_ double _lv , double _lci , double _lco ) { + double _lghk; + double _lz , _leci , _leco ; + _lz = ( 1e-3 ) * 2.0 * FARADAY * _lv / ( R * ( celsius + 273.15 ) ) ; + _leco = _lco * efun ( _threadargscomma_ _lz ) ; + _leci = _lci * efun ( _threadargscomma_ - _lz ) ; + _lghk = ( 1e-3 ) * 2.0 * FARADAY * ( _leci - _leco ) ; + +return _lghk; + } + +static void _hoc_ghk(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = ghk ( _p, _ppvar, _thread, _nt, *getarg(1) , *getarg(2) , *getarg(3) ); + hoc_retpushx(_r); +} + +double efun ( _threadargsprotocomma_ double _lz ) { + double _lefun; + if ( fabs ( _lz ) < 1e-4 ) { + _lefun = 1.0 - _lz / 2.0 ; + } + else { + _lefun = _lz / ( exp ( _lz ) - 1.0 ) ; + } + +return _lefun; + } + +static void _hoc_efun(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = efun ( _p, _ppvar, _thread, _nt, *getarg(1) ); + hoc_retpushx(_r); +} + +double modulationDA ( _threadargsproto_ ) { + double _lmodulationDA; + _lmodulationDA = 1.0 + modDA * ( maxModDA - 1.0 ) * levelDA ; + +return _lmodulationDA; + } + +static void _hoc_modulationDA(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = modulationDA ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +double modulationACh ( _threadargsproto_ ) { + double _lmodulationACh; + _lmodulationACh = 1.0 + modACh * ( maxModACh - 1.0 ) * levelACh ; + +return _lmodulationACh; + } + +static void _hoc_modulationACh(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = modulationACh ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +static int _ode_count(int _type){ return 2;} + +static void _ode_spec(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + cai = _ion_cai; + cao = _ion_cao; + _ode_spec1 (_p, _ppvar, _thread, _nt); + }} + +static void _ode_map(int _ieq, double** _pv, double** _pvdot, double* _pp, Datum* _ppd, double* _atol, int _type) { + double* _p; Datum* _ppvar; + int _i; _p = _pp; _ppvar = _ppd; + _cvode_ieq = _ieq; + for (_i=0; _i < 2; ++_i) { + _pv[_i] = _pp + _slist1[_i]; _pvdot[_i] = _pp + _dlist1[_i]; + _cvode_abstol(_atollist, _atol, _i); + } + } + +static void _ode_matsol_instance1(_threadargsproto_) { + _ode_matsol1 (_p, _ppvar, _thread, _nt); + } + +static void _ode_matsol(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + cai = _ion_cai; + cao = _ion_cao; + _ode_matsol_instance1(_threadargs_); + }} + extern void nrn_update_ion_pointer(Symbol*, Datum*, int, int); + static void _update_ion_pointer(Datum* _ppvar) { + nrn_update_ion_pointer(_ca_sym, _ppvar, 0, 1); + nrn_update_ion_pointer(_ca_sym, _ppvar, 1, 2); + nrn_update_ion_pointer(_ca_sym, _ppvar, 2, 3); + nrn_update_ion_pointer(_ca_sym, _ppvar, 3, 4); + } + +static void initmodel(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + int _i; double _save;{ + h = h0; + m = m0; + { + rates ( _threadargs_ ) ; + m = minf ; + h = hinf ; + } + +} +} + +static void nrn_init(NrnThread* _nt, _Memb_list* _ml, int _type){ +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v = _v; + cai = _ion_cai; + cao = _ion_cao; + initmodel(_p, _ppvar, _thread, _nt); + } +} + +static double _nrn_current(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, double _v){double _current=0.;v=_v;{ { + ica = pbar * m * m * ( h * a + 1.0 - a ) * ghk ( _threadargscomma_ v , cai , cao ) * modulationDA ( _threadargs_ ) * modulationACh ( _threadargs_ ) ; + } + _current += ica; + +} return _current; +} + +static void nrn_cur(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; double _rhs, _v; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + cai = _ion_cai; + cao = _ion_cao; + _g = _nrn_current(_p, _ppvar, _thread, _nt, _v + .001); + { double _dica; + _dica = ica; + _rhs = _nrn_current(_p, _ppvar, _thread, _nt, _v); + _ion_dicadv += (_dica - ica)/.001 ; + } + _g = (_g - _rhs)/.001; + _ion_ica += ica ; +#if CACHEVEC + if (use_cachevec) { + VEC_RHS(_ni[_iml]) -= _rhs; + }else +#endif + { + NODERHS(_nd) -= _rhs; + } + +} + +} + +static void nrn_jacob(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; +#if CACHEVEC + if (use_cachevec) { + VEC_D(_ni[_iml]) += _g; + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + NODED(_nd) += _g; + } + +} + +} + +static void nrn_state(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v = 0.0; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v=_v; +{ + cai = _ion_cai; + cao = _ion_cao; + { states(_p, _ppvar, _thread, _nt); + } }} + +} + +static void terminal(){} + +static void _initlists(){ + double _x; double* _p = &_x; + int _i; static int _first = 1; + if (!_first) return; + _slist1[0] = m_columnindex; _dlist1[0] = Dm_columnindex; + _slist1[1] = h_columnindex; _dlist1[1] = Dh_columnindex; +_first = 0; +} + +#if defined(__cplusplus) +} /* extern "C" */ +#endif + +#if NMODL_TEXT +static const char* nmodl_filename = "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/can_ms.mod"; +static const char* nmodl_file_text = + "TITLE N-type calcium current (Cav2.2)\n" + "\n" + "COMMENT\n" + "\n" + "Neuromodulation is added as functions:\n" + " \n" + " modulationDA = 1 + modDA*(maxModDA-1)*levelDA\n" + "\n" + "where:\n" + " \n" + " modDA [0]: is a switch for turning modulation on or off {1/0}\n" + " maxModDA [1]: is the maximum modulation for this specific channel (read from the param file)\n" + " e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf}\n" + " levelDA [0]: is an additional parameter for scaling modulation. \n" + " Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1}\n" + " \n" + " Further neuromodulators can be added by for example:\n" + " modulationDA = 1 + modDA*(maxModDA-1)\n" + " modulationACh = 1 + modACh*(maxModACh-1)\n" + " ....\n" + "\n" + " etc. for other neuromodulators\n" + " \n" + " \n" + " \n" + "[] == default values\n" + "{} == ranges\n" + " \n" + "ENDCOMMENT\n" + "\n" + "\n" + "UNITS {\n" + " (mV) = (millivolt)\n" + " (mA) = (milliamp)\n" + " (S) = (siemens)\n" + " (molar) = (1/liter)\n" + " (mM) = (millimolar)\n" + " FARADAY = (faraday) (coulomb)\n" + " R = (k-mole) (joule/degC)\n" + "}\n" + "\n" + "NEURON {\n" + " SUFFIX can_ms\n" + " USEION ca READ cai, cao WRITE ica VALENCE 2\n" + " RANGE pbar, ica\n" + " RANGE modDA, maxModDA, levelDA, modACh, maxModACh, levelACh\n" + "}\n" + "\n" + "PARAMETER {\n" + " pbar = 0.0 (cm/s)\n" + " a = 0.21\n" + " :q = 1 : room temperature 22-25 C\n" + " q = 3 : body temperature 35 C\n" + " modDA = 0\n" + " maxModDA = 1\n" + " levelDA = 0\n" + " modACh = 0\n" + " maxModACh = 1\n" + " levelACh = 0\n" + "} \n" + "\n" + "ASSIGNED { \n" + " v (mV)\n" + " ica (mA/cm2)\n" + " eca (mV)\n" + " celsius (degC)\n" + " cai (mM)\n" + " cao (mM)\n" + " minf\n" + " mtau (ms)\n" + " hinf\n" + " htau (ms)\n" + "}\n" + "\n" + "STATE { m h }\n" + "\n" + "BREAKPOINT {\n" + " SOLVE states METHOD cnexp\n" + " ica = pbar*m*m*(h*a+1-a)*ghk(v, cai, cao)*modulationDA()*modulationACh()\n" + "}\n" + "\n" + "INITIAL {\n" + " rates()\n" + " m = minf\n" + " h = hinf\n" + "}\n" + "\n" + "DERIVATIVE states { \n" + " rates()\n" + " m' = (minf-m)/mtau*q\n" + " h' = (hinf-h)/htau*q\n" + "}\n" + "\n" + "PROCEDURE rates() {\n" + " UNITSOFF\n" + " minf = 1/(1+exp((v-(-3))/(-8)))\n" + " mtau = 0.06+1/(exp((v-25)/18)+exp((v-(-31))/(-44)))\n" + " hinf = 1/(1+exp((v-(-74.8))/6.5))\n" + " htau = 70\n" + " UNITSON\n" + "}\n" + "\n" + "FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) {\n" + " LOCAL z, eci, eco\n" + " z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15))\n" + " eco = co*efun(z)\n" + " eci = ci*efun(-z)\n" + " ghk = (1e-3)*2*FARADAY*(eci-eco)\n" + "}\n" + "\n" + "FUNCTION efun(z) {\n" + " if (fabs(z) < 1e-4) {\n" + " efun = 1-z/2\n" + " }else{\n" + " efun = z/(exp(z)-1)\n" + " }\n" + "}\n" + "\n" + "FUNCTION modulationDA() {\n" + " : returns modulation factor\n" + " \n" + " modulationDA = 1 + modDA*(maxModDA-1)*levelDA \n" + "}\n" + "\n" + "FUNCTION modulationACh() {\n" + " : returns modulation factor\n" + " \n" + " modulationACh = 1 + modACh*(maxModACh-1)*levelACh \n" + "}\n" + "\n" + "\n" + "COMMENT\n" + "\n" + "Model is based on mixed data. Activation curve is from neostriatal\n" + "medium spiny neurons of adult P28+ rats [1, Fig.12F], unspecified\n" + "recording temperature. Potentials were not corrected for the liquid\n" + "junction potential, which was estimated to be 7 mV. Activation time\n" + "constant is from the rodent neuron culture (both rat and mouse cells),\n" + "room temperature 22-25 C [2, Fig.15B]. Inactivation data is from human\n" + "(HEK) cells [3, Tab.1, Tab.2], supposedly at room temperature.\n" + "\n" + "Kinetics of m2h type is used [2, Fig.5]. Activation of m^2 is fitted\n" + "to the experimental data [1,4], activation time constant corresponds\n" + "to m^2 already [2]. Original model [5,4] was modified by Alexander\n" + "Kozlov . Activation time constant was refitted to avoid\n" + "singularity in the expression. Temperature correction factor 3 is used\n" + "for body temperature [4,5].\n" + "\n" + "[1] Bargas J, Howe A, Eberwine J, Cao Y, Surmeier DJ (1994) Cellular\n" + "and molecular characterization of Ca2+ currents in acutely isolated,\n" + "adult rat neostriatal neurons. J Neurosci 14(11 Pt 1):6667-86.\n" + "\n" + "[2] Kasai H, Neher E (1992) Dihydropyridine-sensitive and\n" + "omega-conotoxin-sensitive calcium channels in a mammalian\n" + "neuroblastoma-glioma cell line. J Physiol 448:161-88.\n" + "\n" + "[3] McNaughton NC, Randall AD (1997) Electrophysiological properties of\n" + "the human N-type Ca2+ channel: I. Channel gating in Ca2+, Ba2+ and Sr2+\n" + "containing solutions. Neuropharmacology 36(7):895-915.\n" + "\n" + "[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of\n" + "spike timing-dependent calcium influx during corticostriatal upstates. J\n" + "Neurophysiol 110(7):1631-45.\n" + "\n" + "[5] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M,\n" + "O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions\n" + "and entrainment to oscillations in a computational model of the nucleus\n" + "accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95.\n" + "\n" + "ENDCOMMENT\n" + ; +#endif diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/can_ms.o b/examples/example_models/str-dspn-model1/mechanisms/x86_64/can_ms.o new file mode 100644 index 00000000..7e9403d5 Binary files /dev/null and b/examples/example_models/str-dspn-model1/mechanisms/x86_64/can_ms.o differ diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/caq_ms.c b/examples/example_models/str-dspn-model1/mechanisms/x86_64/caq_ms.c new file mode 100644 index 00000000..e98eee86 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/x86_64/caq_ms.c @@ -0,0 +1,656 @@ +/* Created by Language version: 7.7.0 */ +/* VECTORIZED */ +#define NRN_VECTORIZED 1 +#include +#include +#include +#include "mech_api.h" +#undef PI +#define nil 0 +#include "md1redef.h" +#include "section.h" +#include "nrniv_mf.h" +#include "md2redef.h" + +#if METHOD3 +extern int _method3; +#endif + +#if !NRNGPU +#undef exp +#define exp hoc_Exp +extern double hoc_Exp(double); +#endif + +#define nrn_init _nrn_init__caq_ms +#define _nrn_initial _nrn_initial__caq_ms +#define nrn_cur _nrn_cur__caq_ms +#define _nrn_current _nrn_current__caq_ms +#define nrn_jacob _nrn_jacob__caq_ms +#define nrn_state _nrn_state__caq_ms +#define _net_receive _net_receive__caq_ms +#define rates rates__caq_ms +#define states states__caq_ms + +#define _threadargscomma_ _p, _ppvar, _thread, _nt, +#define _threadargsprotocomma_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, +#define _threadargs_ _p, _ppvar, _thread, _nt +#define _threadargsproto_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt + /*SUPPRESS 761*/ + /*SUPPRESS 762*/ + /*SUPPRESS 763*/ + /*SUPPRESS 765*/ + extern double *getarg(); + /* Thread safe. No static _p or _ppvar. */ + +#define t _nt->_t +#define dt _nt->_dt +#define pbar _p[0] +#define pbar_columnindex 0 +#define ica _p[1] +#define ica_columnindex 1 +#define m _p[2] +#define m_columnindex 2 +#define eca _p[3] +#define eca_columnindex 3 +#define cai _p[4] +#define cai_columnindex 4 +#define cao _p[5] +#define cao_columnindex 5 +#define minf _p[6] +#define minf_columnindex 6 +#define mtau _p[7] +#define mtau_columnindex 7 +#define Dm _p[8] +#define Dm_columnindex 8 +#define v _p[9] +#define v_columnindex 9 +#define _g _p[10] +#define _g_columnindex 10 +#define _ion_cai *_ppvar[0]._pval +#define _ion_cao *_ppvar[1]._pval +#define _ion_ica *_ppvar[2]._pval +#define _ion_dicadv *_ppvar[3]._pval + +#if MAC +#if !defined(v) +#define v _mlhv +#endif +#if !defined(h) +#define h _mlhh +#endif +#endif + +#if defined(__cplusplus) +extern "C" { +#endif + static int hoc_nrnpointerindex = -1; + static Datum* _extcall_thread; + static Prop* _extcall_prop; + /* external NEURON variables */ + extern double celsius; + /* declaration of user functions */ + static void _hoc_efun(void); + static void _hoc_ghk(void); + static void _hoc_rates(void); + static int _mechtype; +extern void _nrn_cacheloop_reg(int, int); +extern void hoc_register_prop_size(int, int, int); +extern void hoc_register_limits(int, HocParmLimits*); +extern void hoc_register_units(int, HocParmUnits*); +extern void nrn_promote(Prop*, int, int); +extern Memb_func* memb_func; + +#define NMODL_TEXT 1 +#if NMODL_TEXT +static const char* nmodl_file_text; +static const char* nmodl_filename; +extern void hoc_reg_nmodl_text(int, const char*); +extern void hoc_reg_nmodl_filename(int, const char*); +#endif + + extern void _nrn_setdata_reg(int, void(*)(Prop*)); + static void _setdata(Prop* _prop) { + _extcall_prop = _prop; + } + static void _hoc_setdata() { + Prop *_prop, *hoc_getdata_range(int); + _prop = hoc_getdata_range(_mechtype); + _setdata(_prop); + hoc_retpushx(1.); +} + /* connect user functions to hoc names */ + static VoidFunc hoc_intfunc[] = { + "setdata_caq_ms", _hoc_setdata, + "efun_caq_ms", _hoc_efun, + "ghk_caq_ms", _hoc_ghk, + "rates_caq_ms", _hoc_rates, + 0, 0 +}; +#define efun efun_caq_ms +#define ghk ghk_caq_ms + extern double efun( _threadargsprotocomma_ double ); + extern double ghk( _threadargsprotocomma_ double , double , double ); + /* declare global and static user variables */ +#define q q_caq_ms + double q = 3; + /* some parameters have upper and lower limits */ + static HocParmLimits _hoc_parm_limits[] = { + 0,0,0 +}; + static HocParmUnits _hoc_parm_units[] = { + "pbar_caq_ms", "cm/s", + "ica_caq_ms", "mA/cm2", + 0,0 +}; + static double delta_t = 0.01; + static double m0 = 0; + /* connect global user variables to hoc */ + static DoubScal hoc_scdoub[] = { + "q_caq_ms", &q_caq_ms, + 0,0 +}; + static DoubVec hoc_vdoub[] = { + 0,0,0 +}; + static double _sav_indep; + static void nrn_alloc(Prop*); +static void nrn_init(NrnThread*, _Memb_list*, int); +static void nrn_state(NrnThread*, _Memb_list*, int); + static void nrn_cur(NrnThread*, _Memb_list*, int); +static void nrn_jacob(NrnThread*, _Memb_list*, int); + +static int _ode_count(int); +static void _ode_map(int, double**, double**, double*, Datum*, double*, int); +static void _ode_spec(NrnThread*, _Memb_list*, int); +static void _ode_matsol(NrnThread*, _Memb_list*, int); + +#define _cvode_ieq _ppvar[4]._i + static void _ode_matsol_instance1(_threadargsproto_); + /* connect range variables in _p that hoc is supposed to know about */ + static const char *_mechanism[] = { + "7.7.0", +"caq_ms", + "pbar_caq_ms", + 0, + "ica_caq_ms", + 0, + "m_caq_ms", + 0, + 0}; + static Symbol* _ca_sym; + +extern Prop* need_memb(Symbol*); + +static void nrn_alloc(Prop* _prop) { + Prop *prop_ion; + double *_p; Datum *_ppvar; + _p = nrn_prop_data_alloc(_mechtype, 11, _prop); + /*initialize range parameters*/ + pbar = 0; + _prop->param = _p; + _prop->param_size = 11; + _ppvar = nrn_prop_datum_alloc(_mechtype, 5, _prop); + _prop->dparam = _ppvar; + /*connect ionic variables to this model*/ + prop_ion = need_memb(_ca_sym); + nrn_promote(prop_ion, 1, 0); + _ppvar[0]._pval = &prop_ion->param[1]; /* cai */ + _ppvar[1]._pval = &prop_ion->param[2]; /* cao */ + _ppvar[2]._pval = &prop_ion->param[3]; /* ica */ + _ppvar[3]._pval = &prop_ion->param[4]; /* _ion_dicadv */ + +} + static void _initlists(); + /* some states have an absolute tolerance */ + static Symbol** _atollist; + static HocStateTolerance _hoc_state_tol[] = { + 0,0 +}; + static void _update_ion_pointer(Datum*); + extern Symbol* hoc_lookup(const char*); +extern void _nrn_thread_reg(int, int, void(*)(Datum*)); +extern void _nrn_thread_table_reg(int, void(*)(double*, Datum*, Datum*, NrnThread*, int)); +extern void hoc_register_tolerance(int, HocStateTolerance*, Symbol***); +extern void _cvode_abstol( Symbol**, double*, int); + + void _caq_ms_reg() { + int _vectorized = 1; + _initlists(); + ion_reg("ca", 2.0); + _ca_sym = hoc_lookup("ca_ion"); + register_mech(_mechanism, nrn_alloc,nrn_cur, nrn_jacob, nrn_state, nrn_init, hoc_nrnpointerindex, 1); + _mechtype = nrn_get_mechtype(_mechanism[1]); + _nrn_setdata_reg(_mechtype, _setdata); + _nrn_thread_reg(_mechtype, 2, _update_ion_pointer); + #if NMODL_TEXT + hoc_reg_nmodl_text(_mechtype, nmodl_file_text); + hoc_reg_nmodl_filename(_mechtype, nmodl_filename); +#endif + hoc_register_prop_size(_mechtype, 11, 5); + hoc_register_dparam_semantics(_mechtype, 0, "ca_ion"); + hoc_register_dparam_semantics(_mechtype, 1, "ca_ion"); + hoc_register_dparam_semantics(_mechtype, 2, "ca_ion"); + hoc_register_dparam_semantics(_mechtype, 3, "ca_ion"); + hoc_register_dparam_semantics(_mechtype, 4, "cvodeieq"); + hoc_register_cvode(_mechtype, _ode_count, _ode_map, _ode_spec, _ode_matsol); + hoc_register_tolerance(_mechtype, _hoc_state_tol, &_atollist); + hoc_register_var(hoc_scdoub, hoc_vdoub, hoc_intfunc); + ivoc_help("help ?1 caq_ms /home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/caq_ms.mod\n"); + hoc_register_limits(_mechtype, _hoc_parm_limits); + hoc_register_units(_mechtype, _hoc_parm_units); + } + +#define FARADAY _nrnunit_FARADAY[_nrnunit_use_legacy_] +static double _nrnunit_FARADAY[2] = {0x1.78e555060882cp+16, 96485.3}; /* 96485.3321233100141 */ + +#define R _nrnunit_R[_nrnunit_use_legacy_] +static double _nrnunit_R[2] = {0x1.0a1013e8990bep+3, 8.3145}; /* 8.3144626181532395 */ +static int _reset; +static char *modelname = "Q-type calcium current (Cav2.1)"; + +static int error; +static int _ninits = 0; +static int _match_recurse=1; +static void _modl_cleanup(){ _match_recurse=1;} +static int rates(_threadargsproto_); + +static int _ode_spec1(_threadargsproto_); +/*static int _ode_matsol1(_threadargsproto_);*/ + static int _slist1[1], _dlist1[1]; + static int states(_threadargsproto_); + +/*CVODE*/ + static int _ode_spec1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) {int _reset = 0; { + rates ( _threadargs_ ) ; + Dm = ( minf - m ) / mtau * q ; + } + return _reset; +} + static int _ode_matsol1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + rates ( _threadargs_ ) ; + Dm = Dm / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) )) ; + return 0; +} + /*END CVODE*/ + static int states (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { { + rates ( _threadargs_ ) ; + m = m + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / mtau )*( q ))))*(- ( ( ( ( minf ) ) / mtau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) ) - m) ; + } + return 0; +} + +static int rates ( _threadargsproto_ ) { + minf = 1.0 / ( 1.0 + exp ( ( v - ( - 16.3 ) ) / ( - 7.9 ) ) ) ; + mtau = 1.13 * 2.0 ; + return 0; } + +static void _hoc_rates(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = 1.; + rates ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +double ghk ( _threadargsprotocomma_ double _lv , double _lci , double _lco ) { + double _lghk; + double _lz , _leci , _leco ; + _lz = ( 1e-3 ) * 2.0 * FARADAY * _lv / ( R * ( celsius + 273.15 ) ) ; + _leco = _lco * efun ( _threadargscomma_ _lz ) ; + _leci = _lci * efun ( _threadargscomma_ - _lz ) ; + _lghk = ( 1e-3 ) * 2.0 * FARADAY * ( _leci - _leco ) ; + +return _lghk; + } + +static void _hoc_ghk(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = ghk ( _p, _ppvar, _thread, _nt, *getarg(1) , *getarg(2) , *getarg(3) ); + hoc_retpushx(_r); +} + +double efun ( _threadargsprotocomma_ double _lz ) { + double _lefun; + if ( fabs ( _lz ) < 1e-4 ) { + _lefun = 1.0 - _lz / 2.0 ; + } + else { + _lefun = _lz / ( exp ( _lz ) - 1.0 ) ; + } + +return _lefun; + } + +static void _hoc_efun(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = efun ( _p, _ppvar, _thread, _nt, *getarg(1) ); + hoc_retpushx(_r); +} + +static int _ode_count(int _type){ return 1;} + +static void _ode_spec(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + cai = _ion_cai; + cao = _ion_cao; + _ode_spec1 (_p, _ppvar, _thread, _nt); + }} + +static void _ode_map(int _ieq, double** _pv, double** _pvdot, double* _pp, Datum* _ppd, double* _atol, int _type) { + double* _p; Datum* _ppvar; + int _i; _p = _pp; _ppvar = _ppd; + _cvode_ieq = _ieq; + for (_i=0; _i < 1; ++_i) { + _pv[_i] = _pp + _slist1[_i]; _pvdot[_i] = _pp + _dlist1[_i]; + _cvode_abstol(_atollist, _atol, _i); + } + } + +static void _ode_matsol_instance1(_threadargsproto_) { + _ode_matsol1 (_p, _ppvar, _thread, _nt); + } + +static void _ode_matsol(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + cai = _ion_cai; + cao = _ion_cao; + _ode_matsol_instance1(_threadargs_); + }} + extern void nrn_update_ion_pointer(Symbol*, Datum*, int, int); + static void _update_ion_pointer(Datum* _ppvar) { + nrn_update_ion_pointer(_ca_sym, _ppvar, 0, 1); + nrn_update_ion_pointer(_ca_sym, _ppvar, 1, 2); + nrn_update_ion_pointer(_ca_sym, _ppvar, 2, 3); + nrn_update_ion_pointer(_ca_sym, _ppvar, 3, 4); + } + +static void initmodel(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + int _i; double _save;{ + m = m0; + { + rates ( _threadargs_ ) ; + m = minf ; + } + +} +} + +static void nrn_init(NrnThread* _nt, _Memb_list* _ml, int _type){ +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v = _v; + cai = _ion_cai; + cao = _ion_cao; + initmodel(_p, _ppvar, _thread, _nt); + } +} + +static double _nrn_current(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, double _v){double _current=0.;v=_v;{ { + ica = pbar * m * m * ghk ( _threadargscomma_ v , cai , cao ) ; + } + _current += ica; + +} return _current; +} + +static void nrn_cur(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; double _rhs, _v; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + cai = _ion_cai; + cao = _ion_cao; + _g = _nrn_current(_p, _ppvar, _thread, _nt, _v + .001); + { double _dica; + _dica = ica; + _rhs = _nrn_current(_p, _ppvar, _thread, _nt, _v); + _ion_dicadv += (_dica - ica)/.001 ; + } + _g = (_g - _rhs)/.001; + _ion_ica += ica ; +#if CACHEVEC + if (use_cachevec) { + VEC_RHS(_ni[_iml]) -= _rhs; + }else +#endif + { + NODERHS(_nd) -= _rhs; + } + +} + +} + +static void nrn_jacob(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; +#if CACHEVEC + if (use_cachevec) { + VEC_D(_ni[_iml]) += _g; + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + NODED(_nd) += _g; + } + +} + +} + +static void nrn_state(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v = 0.0; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v=_v; +{ + cai = _ion_cai; + cao = _ion_cao; + { states(_p, _ppvar, _thread, _nt); + } }} + +} + +static void terminal(){} + +static void _initlists(){ + double _x; double* _p = &_x; + int _i; static int _first = 1; + if (!_first) return; + _slist1[0] = m_columnindex; _dlist1[0] = Dm_columnindex; +_first = 0; +} + +#if defined(__cplusplus) +} /* extern "C" */ +#endif + +#if NMODL_TEXT +static const char* nmodl_filename = "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/caq_ms.mod"; +static const char* nmodl_file_text = + "TITLE Q-type calcium current (Cav2.1)\n" + "\n" + "UNITS {\n" + " (mV) = (millivolt)\n" + " (mA) = (milliamp)\n" + " (S) = (siemens)\n" + " (molar) = (1/liter)\n" + " (mM) = (millimolar)\n" + " FARADAY = (faraday) (coulomb)\n" + " R = (k-mole) (joule/degC)\n" + "}\n" + "\n" + "NEURON {\n" + " SUFFIX caq_ms\n" + " USEION ca READ cai, cao WRITE ica VALENCE 2\n" + " RANGE pbar, ica\n" + "}\n" + "\n" + "PARAMETER {\n" + " pbar = 0.0 (cm/s)\n" + " :q = 1 : room temperature 22 C\n" + " q = 3 : body temperature 35 C\n" + "} \n" + "\n" + "ASSIGNED { \n" + " v (mV)\n" + " ica (mA/cm2)\n" + " eca (mV)\n" + " celsius (degC)\n" + " cai (mM)\n" + " cao (mM)\n" + " minf\n" + " mtau (ms)\n" + "}\n" + "\n" + "STATE { m }\n" + "\n" + "BREAKPOINT {\n" + " SOLVE states METHOD cnexp\n" + " ica = pbar*m*m*ghk(v, cai, cao)\n" + "}\n" + "\n" + "INITIAL {\n" + " rates()\n" + " m = minf\n" + "}\n" + "\n" + "DERIVATIVE states { \n" + " rates()\n" + " m' = (minf-m)/mtau*q\n" + "}\n" + "\n" + "PROCEDURE rates() {\n" + " UNITSOFF\n" + " minf = 1/(1+exp((v-(-16.3))/(-7.9)))\n" + " mtau = 1.13*2\n" + " UNITSON\n" + "}\n" + "\n" + "FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) {\n" + " LOCAL z, eci, eco\n" + " z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15))\n" + " eco = co*efun(z)\n" + " eci = ci*efun(-z)\n" + " ghk = (1e-3)*2*FARADAY*(eci-eco)\n" + "}\n" + "\n" + "FUNCTION efun(z) {\n" + " if (fabs(z) < 1e-4) {\n" + " efun = 1-z/2\n" + " }else{\n" + " efun = z/(exp(z)-1)\n" + " }\n" + "}\n" + "\n" + "COMMENT\n" + "\n" + "Activation curve was reconstructed for cultured NAc neurons from P5-P32\n" + "Charles River rat pups [1]. Activation time constant was measured in\n" + "culture neurons from cerebellum of P2-P5 rat pups [2] at room temperature\n" + "22 C.\n" + "\n" + "Original NEURON model by Wolf (2005) [3] was modified by Alexander Kozlov\n" + ". Activation curve was fitted to m2 kinetics [4],\n" + "activation time constant was scaled up as well.\n" + "\n" + "[1] Churchill D, Macvicar BA (1998) Biophysical and pharmacological\n" + "characterization of voltage-dependent Ca2+ channels in neurons isolated\n" + "from rat nucleus accumbens. J Neurophysiol 79(2):635-47.\n" + "\n" + "[2] Randall A, Tsien RW (1995) Pharmacological dissection of multiple\n" + "types of Ca2+ channel currents in rat cerebellar granule neurons. J\n" + "Neurosci 15(4):2995-3012.\n" + "\n" + "[3] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M,\n" + "O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions\n" + "and entrainment to oscillations in a computational model of the nucleus\n" + "accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95.\n" + "\n" + "[4] Brown AM, Schwindt PC, Crill WE (1993) Voltage dependence and\n" + "activation kinetics of pharmacologically defined components of the\n" + "high-threshold calcium current in rat neocortical neurons. J Neurophysiol\n" + "70(4):1530-43.\n" + "\n" + "ENDCOMMENT\n" + ; +#endif diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/caq_ms.o b/examples/example_models/str-dspn-model1/mechanisms/x86_64/caq_ms.o new file mode 100644 index 00000000..5dedc411 Binary files /dev/null and b/examples/example_models/str-dspn-model1/mechanisms/x86_64/caq_ms.o differ diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/car_ms.c b/examples/example_models/str-dspn-model1/mechanisms/x86_64/car_ms.c new file mode 100644 index 00000000..e7904237 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/x86_64/car_ms.c @@ -0,0 +1,756 @@ +/* Created by Language version: 7.7.0 */ +/* VECTORIZED */ +#define NRN_VECTORIZED 1 +#include +#include +#include +#include "mech_api.h" +#undef PI +#define nil 0 +#include "md1redef.h" +#include "section.h" +#include "nrniv_mf.h" +#include "md2redef.h" + +#if METHOD3 +extern int _method3; +#endif + +#if !NRNGPU +#undef exp +#define exp hoc_Exp +extern double hoc_Exp(double); +#endif + +#define nrn_init _nrn_init__car_ms +#define _nrn_initial _nrn_initial__car_ms +#define nrn_cur _nrn_cur__car_ms +#define _nrn_current _nrn_current__car_ms +#define nrn_jacob _nrn_jacob__car_ms +#define nrn_state _nrn_state__car_ms +#define _net_receive _net_receive__car_ms +#define rates rates__car_ms +#define states states__car_ms + +#define _threadargscomma_ _p, _ppvar, _thread, _nt, +#define _threadargsprotocomma_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, +#define _threadargs_ _p, _ppvar, _thread, _nt +#define _threadargsproto_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt + /*SUPPRESS 761*/ + /*SUPPRESS 762*/ + /*SUPPRESS 763*/ + /*SUPPRESS 765*/ + extern double *getarg(); + /* Thread safe. No static _p or _ppvar. */ + +#define t _nt->_t +#define dt _nt->_dt +#define pbar _p[0] +#define pbar_columnindex 0 +#define modDA _p[1] +#define modDA_columnindex 1 +#define maxModDA _p[2] +#define maxModDA_columnindex 2 +#define levelDA _p[3] +#define levelDA_columnindex 3 +#define ica _p[4] +#define ica_columnindex 4 +#define m _p[5] +#define m_columnindex 5 +#define h _p[6] +#define h_columnindex 6 +#define eca _p[7] +#define eca_columnindex 7 +#define cai _p[8] +#define cai_columnindex 8 +#define cao _p[9] +#define cao_columnindex 9 +#define minf _p[10] +#define minf_columnindex 10 +#define mtau _p[11] +#define mtau_columnindex 11 +#define hinf _p[12] +#define hinf_columnindex 12 +#define htau _p[13] +#define htau_columnindex 13 +#define Dm _p[14] +#define Dm_columnindex 14 +#define Dh _p[15] +#define Dh_columnindex 15 +#define v _p[16] +#define v_columnindex 16 +#define _g _p[17] +#define _g_columnindex 17 +#define _ion_cai *_ppvar[0]._pval +#define _ion_cao *_ppvar[1]._pval +#define _ion_ica *_ppvar[2]._pval +#define _ion_dicadv *_ppvar[3]._pval + +#if MAC +#if !defined(v) +#define v _mlhv +#endif +#if !defined(h) +#define h _mlhh +#endif +#endif + +#if defined(__cplusplus) +extern "C" { +#endif + static int hoc_nrnpointerindex = -1; + static Datum* _extcall_thread; + static Prop* _extcall_prop; + /* external NEURON variables */ + extern double celsius; + /* declaration of user functions */ + static void _hoc_efun(void); + static void _hoc_ghk(void); + static void _hoc_modulationDA(void); + static void _hoc_rates(void); + static int _mechtype; +extern void _nrn_cacheloop_reg(int, int); +extern void hoc_register_prop_size(int, int, int); +extern void hoc_register_limits(int, HocParmLimits*); +extern void hoc_register_units(int, HocParmUnits*); +extern void nrn_promote(Prop*, int, int); +extern Memb_func* memb_func; + +#define NMODL_TEXT 1 +#if NMODL_TEXT +static const char* nmodl_file_text; +static const char* nmodl_filename; +extern void hoc_reg_nmodl_text(int, const char*); +extern void hoc_reg_nmodl_filename(int, const char*); +#endif + + extern void _nrn_setdata_reg(int, void(*)(Prop*)); + static void _setdata(Prop* _prop) { + _extcall_prop = _prop; + } + static void _hoc_setdata() { + Prop *_prop, *hoc_getdata_range(int); + _prop = hoc_getdata_range(_mechtype); + _setdata(_prop); + hoc_retpushx(1.); +} + /* connect user functions to hoc names */ + static VoidFunc hoc_intfunc[] = { + "setdata_car_ms", _hoc_setdata, + "efun_car_ms", _hoc_efun, + "ghk_car_ms", _hoc_ghk, + "modulationDA_car_ms", _hoc_modulationDA, + "rates_car_ms", _hoc_rates, + 0, 0 +}; +#define efun efun_car_ms +#define ghk ghk_car_ms +#define modulationDA modulationDA_car_ms + extern double efun( _threadargsprotocomma_ double ); + extern double ghk( _threadargsprotocomma_ double , double , double ); + extern double modulationDA( _threadargsproto_ ); + /* declare global and static user variables */ +#define q q_car_ms + double q = 3; + /* some parameters have upper and lower limits */ + static HocParmLimits _hoc_parm_limits[] = { + 0,0,0 +}; + static HocParmUnits _hoc_parm_units[] = { + "pbar_car_ms", "cm/s", + "ica_car_ms", "mA/cm2", + 0,0 +}; + static double delta_t = 0.01; + static double h0 = 0; + static double m0 = 0; + /* connect global user variables to hoc */ + static DoubScal hoc_scdoub[] = { + "q_car_ms", &q_car_ms, + 0,0 +}; + static DoubVec hoc_vdoub[] = { + 0,0,0 +}; + static double _sav_indep; + static void nrn_alloc(Prop*); +static void nrn_init(NrnThread*, _Memb_list*, int); +static void nrn_state(NrnThread*, _Memb_list*, int); + static void nrn_cur(NrnThread*, _Memb_list*, int); +static void nrn_jacob(NrnThread*, _Memb_list*, int); + +static int _ode_count(int); +static void _ode_map(int, double**, double**, double*, Datum*, double*, int); +static void _ode_spec(NrnThread*, _Memb_list*, int); +static void _ode_matsol(NrnThread*, _Memb_list*, int); + +#define _cvode_ieq _ppvar[4]._i + static void _ode_matsol_instance1(_threadargsproto_); + /* connect range variables in _p that hoc is supposed to know about */ + static const char *_mechanism[] = { + "7.7.0", +"car_ms", + "pbar_car_ms", + "modDA_car_ms", + "maxModDA_car_ms", + "levelDA_car_ms", + 0, + "ica_car_ms", + 0, + "m_car_ms", + "h_car_ms", + 0, + 0}; + static Symbol* _ca_sym; + +extern Prop* need_memb(Symbol*); + +static void nrn_alloc(Prop* _prop) { + Prop *prop_ion; + double *_p; Datum *_ppvar; + _p = nrn_prop_data_alloc(_mechtype, 18, _prop); + /*initialize range parameters*/ + pbar = 0; + modDA = 0; + maxModDA = 1; + levelDA = 0; + _prop->param = _p; + _prop->param_size = 18; + _ppvar = nrn_prop_datum_alloc(_mechtype, 5, _prop); + _prop->dparam = _ppvar; + /*connect ionic variables to this model*/ + prop_ion = need_memb(_ca_sym); + nrn_promote(prop_ion, 1, 0); + _ppvar[0]._pval = &prop_ion->param[1]; /* cai */ + _ppvar[1]._pval = &prop_ion->param[2]; /* cao */ + _ppvar[2]._pval = &prop_ion->param[3]; /* ica */ + _ppvar[3]._pval = &prop_ion->param[4]; /* _ion_dicadv */ + +} + static void _initlists(); + /* some states have an absolute tolerance */ + static Symbol** _atollist; + static HocStateTolerance _hoc_state_tol[] = { + 0,0 +}; + static void _update_ion_pointer(Datum*); + extern Symbol* hoc_lookup(const char*); +extern void _nrn_thread_reg(int, int, void(*)(Datum*)); +extern void _nrn_thread_table_reg(int, void(*)(double*, Datum*, Datum*, NrnThread*, int)); +extern void hoc_register_tolerance(int, HocStateTolerance*, Symbol***); +extern void _cvode_abstol( Symbol**, double*, int); + + void _car_ms_reg() { + int _vectorized = 1; + _initlists(); + ion_reg("ca", 2.0); + _ca_sym = hoc_lookup("ca_ion"); + register_mech(_mechanism, nrn_alloc,nrn_cur, nrn_jacob, nrn_state, nrn_init, hoc_nrnpointerindex, 1); + _mechtype = nrn_get_mechtype(_mechanism[1]); + _nrn_setdata_reg(_mechtype, _setdata); + _nrn_thread_reg(_mechtype, 2, _update_ion_pointer); + #if NMODL_TEXT + hoc_reg_nmodl_text(_mechtype, nmodl_file_text); + hoc_reg_nmodl_filename(_mechtype, nmodl_filename); +#endif + hoc_register_prop_size(_mechtype, 18, 5); + hoc_register_dparam_semantics(_mechtype, 0, "ca_ion"); + hoc_register_dparam_semantics(_mechtype, 1, "ca_ion"); + hoc_register_dparam_semantics(_mechtype, 2, "ca_ion"); + hoc_register_dparam_semantics(_mechtype, 3, "ca_ion"); + hoc_register_dparam_semantics(_mechtype, 4, "cvodeieq"); + hoc_register_cvode(_mechtype, _ode_count, _ode_map, _ode_spec, _ode_matsol); + hoc_register_tolerance(_mechtype, _hoc_state_tol, &_atollist); + hoc_register_var(hoc_scdoub, hoc_vdoub, hoc_intfunc); + ivoc_help("help ?1 car_ms /home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/car_ms.mod\n"); + hoc_register_limits(_mechtype, _hoc_parm_limits); + hoc_register_units(_mechtype, _hoc_parm_units); + } + +#define FARADAY _nrnunit_FARADAY[_nrnunit_use_legacy_] +static double _nrnunit_FARADAY[2] = {0x1.78e555060882cp+16, 96485.3}; /* 96485.3321233100141 */ + +#define R _nrnunit_R[_nrnunit_use_legacy_] +static double _nrnunit_R[2] = {0x1.0a1013e8990bep+3, 8.3145}; /* 8.3144626181532395 */ +static int _reset; +static char *modelname = "R-type calcium current (Cav2.3)"; + +static int error; +static int _ninits = 0; +static int _match_recurse=1; +static void _modl_cleanup(){ _match_recurse=1;} +static int rates(_threadargsproto_); + +static int _ode_spec1(_threadargsproto_); +/*static int _ode_matsol1(_threadargsproto_);*/ + static int _slist1[2], _dlist1[2]; + static int states(_threadargsproto_); + +/*CVODE*/ + static int _ode_spec1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) {int _reset = 0; { + rates ( _threadargs_ ) ; + Dm = ( minf - m ) / mtau * q ; + Dh = ( hinf - h ) / htau * q ; + } + return _reset; +} + static int _ode_matsol1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + rates ( _threadargs_ ) ; + Dm = Dm / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) )) ; + Dh = Dh / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / htau )*( q ) )) ; + return 0; +} + /*END CVODE*/ + static int states (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { { + rates ( _threadargs_ ) ; + m = m + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / mtau )*( q ))))*(- ( ( ( ( minf ) ) / mtau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) ) - m) ; + h = h + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / htau )*( q ))))*(- ( ( ( ( hinf ) ) / htau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / htau )*( q ) ) - h) ; + } + return 0; +} + +static int rates ( _threadargsproto_ ) { + minf = 1.0 / ( 1.0 + exp ( ( v - ( - 29.0 ) ) / ( - 9.6 ) ) ) ; + mtau = 5.1 ; + hinf = 1.0 / ( 1.0 + exp ( ( v - ( - 33.3 ) ) / 17.0 ) ) ; + htau = 22.0 + 80.0 / ( 1.0 + exp ( ( v - ( - 19.0 ) ) / 5.0 ) ) ; + return 0; } + +static void _hoc_rates(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = 1.; + rates ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +double ghk ( _threadargsprotocomma_ double _lv , double _lci , double _lco ) { + double _lghk; + double _lz , _leci , _leco ; + _lz = ( 1e-3 ) * 2.0 * FARADAY * _lv / ( R * ( celsius + 273.15 ) ) ; + _leco = _lco * efun ( _threadargscomma_ _lz ) ; + _leci = _lci * efun ( _threadargscomma_ - _lz ) ; + _lghk = ( 1e-3 ) * 2.0 * FARADAY * ( _leci - _leco ) ; + +return _lghk; + } + +static void _hoc_ghk(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = ghk ( _p, _ppvar, _thread, _nt, *getarg(1) , *getarg(2) , *getarg(3) ); + hoc_retpushx(_r); +} + +double efun ( _threadargsprotocomma_ double _lz ) { + double _lefun; + if ( fabs ( _lz ) < 1e-4 ) { + _lefun = 1.0 - _lz / 2.0 ; + } + else { + _lefun = _lz / ( exp ( _lz ) - 1.0 ) ; + } + +return _lefun; + } + +static void _hoc_efun(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = efun ( _p, _ppvar, _thread, _nt, *getarg(1) ); + hoc_retpushx(_r); +} + +double modulationDA ( _threadargsproto_ ) { + double _lmodulationDA; + _lmodulationDA = 1.0 + modDA * ( maxModDA - 1.0 ) * levelDA ; + +return _lmodulationDA; + } + +static void _hoc_modulationDA(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = modulationDA ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +static int _ode_count(int _type){ return 2;} + +static void _ode_spec(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + cai = _ion_cai; + cao = _ion_cao; + _ode_spec1 (_p, _ppvar, _thread, _nt); + }} + +static void _ode_map(int _ieq, double** _pv, double** _pvdot, double* _pp, Datum* _ppd, double* _atol, int _type) { + double* _p; Datum* _ppvar; + int _i; _p = _pp; _ppvar = _ppd; + _cvode_ieq = _ieq; + for (_i=0; _i < 2; ++_i) { + _pv[_i] = _pp + _slist1[_i]; _pvdot[_i] = _pp + _dlist1[_i]; + _cvode_abstol(_atollist, _atol, _i); + } + } + +static void _ode_matsol_instance1(_threadargsproto_) { + _ode_matsol1 (_p, _ppvar, _thread, _nt); + } + +static void _ode_matsol(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + cai = _ion_cai; + cao = _ion_cao; + _ode_matsol_instance1(_threadargs_); + }} + extern void nrn_update_ion_pointer(Symbol*, Datum*, int, int); + static void _update_ion_pointer(Datum* _ppvar) { + nrn_update_ion_pointer(_ca_sym, _ppvar, 0, 1); + nrn_update_ion_pointer(_ca_sym, _ppvar, 1, 2); + nrn_update_ion_pointer(_ca_sym, _ppvar, 2, 3); + nrn_update_ion_pointer(_ca_sym, _ppvar, 3, 4); + } + +static void initmodel(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + int _i; double _save;{ + h = h0; + m = m0; + { + rates ( _threadargs_ ) ; + m = minf ; + h = hinf ; + } + +} +} + +static void nrn_init(NrnThread* _nt, _Memb_list* _ml, int _type){ +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v = _v; + cai = _ion_cai; + cao = _ion_cao; + initmodel(_p, _ppvar, _thread, _nt); + } +} + +static double _nrn_current(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, double _v){double _current=0.;v=_v;{ { + ica = pbar * m * m * m * h * ghk ( _threadargscomma_ v , cai , cao ) * modulationDA ( _threadargs_ ) ; + } + _current += ica; + +} return _current; +} + +static void nrn_cur(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; double _rhs, _v; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + cai = _ion_cai; + cao = _ion_cao; + _g = _nrn_current(_p, _ppvar, _thread, _nt, _v + .001); + { double _dica; + _dica = ica; + _rhs = _nrn_current(_p, _ppvar, _thread, _nt, _v); + _ion_dicadv += (_dica - ica)/.001 ; + } + _g = (_g - _rhs)/.001; + _ion_ica += ica ; +#if CACHEVEC + if (use_cachevec) { + VEC_RHS(_ni[_iml]) -= _rhs; + }else +#endif + { + NODERHS(_nd) -= _rhs; + } + +} + +} + +static void nrn_jacob(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; +#if CACHEVEC + if (use_cachevec) { + VEC_D(_ni[_iml]) += _g; + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + NODED(_nd) += _g; + } + +} + +} + +static void nrn_state(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v = 0.0; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v=_v; +{ + cai = _ion_cai; + cao = _ion_cao; + { states(_p, _ppvar, _thread, _nt); + } }} + +} + +static void terminal(){} + +static void _initlists(){ + double _x; double* _p = &_x; + int _i; static int _first = 1; + if (!_first) return; + _slist1[0] = m_columnindex; _dlist1[0] = Dm_columnindex; + _slist1[1] = h_columnindex; _dlist1[1] = Dh_columnindex; +_first = 0; +} + +#if defined(__cplusplus) +} /* extern "C" */ +#endif + +#if NMODL_TEXT +static const char* nmodl_filename = "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/car_ms.mod"; +static const char* nmodl_file_text = + "TITLE R-type calcium current (Cav2.3)\n" + "\n" + "COMMENT\n" + "\n" + "Neuromodulation is added as functions:\n" + " \n" + " modulationDA = 1 + modDA*(maxModDA-1)*levelDA\n" + "\n" + "where:\n" + " \n" + " modDA [0]: is a switch for turning modulation on or off {1/0}\n" + " maxModDA [1]: is the maximum modulation for this specific channel (read from the param file)\n" + " e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf}\n" + " levelDA [0]: is an additional parameter for scaling modulation. \n" + " Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1}\n" + " \n" + " Further neuromodulators can be added by for example:\n" + " modulationDA = 1 + modDA*(maxModDA-1)\n" + " modulationACh = 1 + modACh*(maxModACh-1)\n" + " ....\n" + "\n" + " etc. for other neuromodulators\n" + " \n" + " \n" + " \n" + "[] == default values\n" + "{} == ranges\n" + "\n" + "ENDCOMMENT\n" + "\n" + "\n" + "UNITS {\n" + " (mV) = (millivolt)\n" + " (mA) = (milliamp)\n" + " (S) = (siemens)\n" + " (molar) = (1/liter)\n" + " (mM) = (millimolar)\n" + " FARADAY = (faraday) (coulomb)\n" + " R = (k-mole) (joule/degC)\n" + "}\n" + "\n" + "NEURON {\n" + " SUFFIX car_ms\n" + " USEION ca READ cai, cao WRITE ica VALENCE 2\n" + " RANGE pbar, ica\n" + " RANGE modDA, maxModDA, levelDA\n" + "}\n" + "\n" + "PARAMETER {\n" + " pbar = 0.0 (cm/s)\n" + " :q = 1 : room temperature 22 C\n" + " q = 3 : body temperature 35 C\n" + " modDA = 0\n" + " maxModDA = 1\n" + " levelDA = 0\n" + "} \n" + "\n" + "ASSIGNED { \n" + " v (mV)\n" + " ica (mA/cm2)\n" + " eca (mV)\n" + " celsius (degC)\n" + " cai (mM)\n" + " cao (mM)\n" + " minf\n" + " mtau (ms)\n" + " hinf\n" + " htau (ms)\n" + "}\n" + "\n" + "STATE { m h }\n" + "\n" + "BREAKPOINT {\n" + " SOLVE states METHOD cnexp\n" + " ica = pbar*m*m*m*h*ghk(v, cai, cao)*modulationDA()\n" + "}\n" + "\n" + "INITIAL {\n" + " rates()\n" + " m = minf\n" + " h = hinf\n" + "}\n" + "\n" + "DERIVATIVE states { \n" + " rates()\n" + " m' = (minf-m)/mtau*q\n" + " h' = (hinf-h)/htau*q\n" + "}\n" + "\n" + "PROCEDURE rates() {\n" + " UNITSOFF\n" + " minf = 1/(1+exp((v-(-29))/(-9.6)))\n" + " mtau = 5.1\n" + " hinf = 1/(1+exp((v-(-33.3))/17))\n" + " htau = 22+80/(1+exp((v-(-19))/5))\n" + " UNITSON\n" + "}\n" + "\n" + "FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) {\n" + " LOCAL z, eci, eco\n" + " z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15))\n" + " eco = co*efun(z)\n" + " eci = ci*efun(-z)\n" + " ghk = (1e-3)*2*FARADAY*(eci-eco)\n" + "}\n" + "\n" + "FUNCTION efun(z) {\n" + " if (fabs(z) < 1e-4) {\n" + " efun = 1-z/2\n" + " }else{\n" + " efun = z/(exp(z)-1)\n" + " }\n" + "}\n" + "\n" + "\n" + "FUNCTION modulationDA() {\n" + " : returns modulation factor\n" + " \n" + " modulationDA = 1 + modDA*(maxModDA-1)*levelDA \n" + "}\n" + "\n" + "\n" + "COMMENT\n" + "\n" + "Original data by Foehring et al (2000) [1] for dissociated MSNs from\n" + "P28-P42 Sprague-Dawley rat brain. Unspecified recording temperature. The\n" + "liquid junction potential was around 8 mV and was not corrected. Kinetics\n" + "of m3h type was fitted. Inactivation time constants were measured in\n" + "neurons from endopiriform nucleus of P7-P21 Hartley guinea pigs [2]\n" + "at room temperature 22 C.\n" + "\n" + "Original NEURON model by Wolf (2005) [3] modified by Alexander Kozlov\n" + ". Activation curve fitted to m^3 kinetics as in [4],\n" + "activation time constant matched m^3 originally [1]. Smooth fit of\n" + "inactivation time constant from [2,3].\n" + "\n" + "[1] Foehring RC, Mermelstein PG, Song WJ, Ulrich S, Surmeier DJ\n" + "(2000) Unique properties of R-type calcium currents in neocortical and\n" + "neostriatal neurons. J Neurophysiol 84(5):2225-36.\n" + "\n" + "[2] Brevi S, de Curtis M, Magistretti J (2001) Pharmacological and\n" + "biophysical characterization of voltage-gated calcium currents in the\n" + "endopiriform nucleus of the guinea pig. J Neurophysiol 85(5):2076-87.\n" + "\n" + "[3] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M,\n" + "O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions\n" + "and entrainment to oscillations in a computational model of the nucleus\n" + "accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95.\n" + "\n" + "[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of\n" + "spike timing-dependent calcium influx during corticostriatal upstates. J\n" + "Neurophysiol 110(7):1631-45.\n" + "\n" + "ENDCOMMENT\n" + ; +#endif diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/car_ms.o b/examples/example_models/str-dspn-model1/mechanisms/x86_64/car_ms.o new file mode 100644 index 00000000..48b1a76d Binary files /dev/null and b/examples/example_models/str-dspn-model1/mechanisms/x86_64/car_ms.o differ diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/cat32_ms.c b/examples/example_models/str-dspn-model1/mechanisms/x86_64/cat32_ms.c new file mode 100644 index 00000000..3840f87c --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/x86_64/cat32_ms.c @@ -0,0 +1,649 @@ +/* Created by Language version: 7.7.0 */ +/* VECTORIZED */ +#define NRN_VECTORIZED 1 +#include +#include +#include +#include "mech_api.h" +#undef PI +#define nil 0 +#include "md1redef.h" +#include "section.h" +#include "nrniv_mf.h" +#include "md2redef.h" + +#if METHOD3 +extern int _method3; +#endif + +#if !NRNGPU +#undef exp +#define exp hoc_Exp +extern double hoc_Exp(double); +#endif + +#define nrn_init _nrn_init__cat32_ms +#define _nrn_initial _nrn_initial__cat32_ms +#define nrn_cur _nrn_cur__cat32_ms +#define _nrn_current _nrn_current__cat32_ms +#define nrn_jacob _nrn_jacob__cat32_ms +#define nrn_state _nrn_state__cat32_ms +#define _net_receive _net_receive__cat32_ms +#define rates rates__cat32_ms +#define states states__cat32_ms + +#define _threadargscomma_ _p, _ppvar, _thread, _nt, +#define _threadargsprotocomma_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, +#define _threadargs_ _p, _ppvar, _thread, _nt +#define _threadargsproto_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt + /*SUPPRESS 761*/ + /*SUPPRESS 762*/ + /*SUPPRESS 763*/ + /*SUPPRESS 765*/ + extern double *getarg(); + /* Thread safe. No static _p or _ppvar. */ + +#define t _nt->_t +#define dt _nt->_dt +#define pbar _p[0] +#define pbar_columnindex 0 +#define ical _p[1] +#define ical_columnindex 1 +#define m _p[2] +#define m_columnindex 2 +#define h _p[3] +#define h_columnindex 3 +#define ecal _p[4] +#define ecal_columnindex 4 +#define cali _p[5] +#define cali_columnindex 5 +#define calo _p[6] +#define calo_columnindex 6 +#define minf _p[7] +#define minf_columnindex 7 +#define mtau _p[8] +#define mtau_columnindex 8 +#define hinf _p[9] +#define hinf_columnindex 9 +#define htau _p[10] +#define htau_columnindex 10 +#define Dm _p[11] +#define Dm_columnindex 11 +#define Dh _p[12] +#define Dh_columnindex 12 +#define v _p[13] +#define v_columnindex 13 +#define _g _p[14] +#define _g_columnindex 14 +#define _ion_cali *_ppvar[0]._pval +#define _ion_calo *_ppvar[1]._pval +#define _ion_ical *_ppvar[2]._pval +#define _ion_dicaldv *_ppvar[3]._pval + +#if MAC +#if !defined(v) +#define v _mlhv +#endif +#if !defined(h) +#define h _mlhh +#endif +#endif + +#if defined(__cplusplus) +extern "C" { +#endif + static int hoc_nrnpointerindex = -1; + static Datum* _extcall_thread; + static Prop* _extcall_prop; + /* external NEURON variables */ + extern double celsius; + /* declaration of user functions */ + static void _hoc_ghk(void); + static void _hoc_rates(void); + static int _mechtype; +extern void _nrn_cacheloop_reg(int, int); +extern void hoc_register_prop_size(int, int, int); +extern void hoc_register_limits(int, HocParmLimits*); +extern void hoc_register_units(int, HocParmUnits*); +extern void nrn_promote(Prop*, int, int); +extern Memb_func* memb_func; + +#define NMODL_TEXT 1 +#if NMODL_TEXT +static const char* nmodl_file_text; +static const char* nmodl_filename; +extern void hoc_reg_nmodl_text(int, const char*); +extern void hoc_reg_nmodl_filename(int, const char*); +#endif + + extern void _nrn_setdata_reg(int, void(*)(Prop*)); + static void _setdata(Prop* _prop) { + _extcall_prop = _prop; + } + static void _hoc_setdata() { + Prop *_prop, *hoc_getdata_range(int); + _prop = hoc_getdata_range(_mechtype); + _setdata(_prop); + hoc_retpushx(1.); +} + /* connect user functions to hoc names */ + static VoidFunc hoc_intfunc[] = { + "setdata_cat32_ms", _hoc_setdata, + "ghk_cat32_ms", _hoc_ghk, + "rates_cat32_ms", _hoc_rates, + 0, 0 +}; +#define ghk ghk_cat32_ms + extern double ghk( _threadargsprotocomma_ double , double , double ); + /* declare global and static user variables */ +#define q q_cat32_ms + double q = 3; + /* some parameters have upper and lower limits */ + static HocParmLimits _hoc_parm_limits[] = { + 0,0,0 +}; + static HocParmUnits _hoc_parm_units[] = { + "pbar_cat32_ms", "cm/s", + "ical_cat32_ms", "mA/cm2", + 0,0 +}; + static double delta_t = 0.01; + static double h0 = 0; + static double m0 = 0; + /* connect global user variables to hoc */ + static DoubScal hoc_scdoub[] = { + "q_cat32_ms", &q_cat32_ms, + 0,0 +}; + static DoubVec hoc_vdoub[] = { + 0,0,0 +}; + static double _sav_indep; + static void nrn_alloc(Prop*); +static void nrn_init(NrnThread*, _Memb_list*, int); +static void nrn_state(NrnThread*, _Memb_list*, int); + static void nrn_cur(NrnThread*, _Memb_list*, int); +static void nrn_jacob(NrnThread*, _Memb_list*, int); + +static int _ode_count(int); +static void _ode_map(int, double**, double**, double*, Datum*, double*, int); +static void _ode_spec(NrnThread*, _Memb_list*, int); +static void _ode_matsol(NrnThread*, _Memb_list*, int); + +#define _cvode_ieq _ppvar[4]._i + static void _ode_matsol_instance1(_threadargsproto_); + /* connect range variables in _p that hoc is supposed to know about */ + static const char *_mechanism[] = { + "7.7.0", +"cat32_ms", + "pbar_cat32_ms", + 0, + "ical_cat32_ms", + 0, + "m_cat32_ms", + "h_cat32_ms", + 0, + 0}; + static Symbol* _cal_sym; + +extern Prop* need_memb(Symbol*); + +static void nrn_alloc(Prop* _prop) { + Prop *prop_ion; + double *_p; Datum *_ppvar; + _p = nrn_prop_data_alloc(_mechtype, 15, _prop); + /*initialize range parameters*/ + pbar = 0; + _prop->param = _p; + _prop->param_size = 15; + _ppvar = nrn_prop_datum_alloc(_mechtype, 5, _prop); + _prop->dparam = _ppvar; + /*connect ionic variables to this model*/ + prop_ion = need_memb(_cal_sym); + nrn_promote(prop_ion, 1, 0); + _ppvar[0]._pval = &prop_ion->param[1]; /* cali */ + _ppvar[1]._pval = &prop_ion->param[2]; /* calo */ + _ppvar[2]._pval = &prop_ion->param[3]; /* ical */ + _ppvar[3]._pval = &prop_ion->param[4]; /* _ion_dicaldv */ + +} + static void _initlists(); + /* some states have an absolute tolerance */ + static Symbol** _atollist; + static HocStateTolerance _hoc_state_tol[] = { + 0,0 +}; + static void _update_ion_pointer(Datum*); + extern Symbol* hoc_lookup(const char*); +extern void _nrn_thread_reg(int, int, void(*)(Datum*)); +extern void _nrn_thread_table_reg(int, void(*)(double*, Datum*, Datum*, NrnThread*, int)); +extern void hoc_register_tolerance(int, HocStateTolerance*, Symbol***); +extern void _cvode_abstol( Symbol**, double*, int); + + void _cat32_ms_reg() { + int _vectorized = 1; + _initlists(); + ion_reg("cal", 2.0); + _cal_sym = hoc_lookup("cal_ion"); + register_mech(_mechanism, nrn_alloc,nrn_cur, nrn_jacob, nrn_state, nrn_init, hoc_nrnpointerindex, 1); + _mechtype = nrn_get_mechtype(_mechanism[1]); + _nrn_setdata_reg(_mechtype, _setdata); + _nrn_thread_reg(_mechtype, 2, _update_ion_pointer); + #if NMODL_TEXT + hoc_reg_nmodl_text(_mechtype, nmodl_file_text); + hoc_reg_nmodl_filename(_mechtype, nmodl_filename); +#endif + hoc_register_prop_size(_mechtype, 15, 5); + hoc_register_dparam_semantics(_mechtype, 0, "cal_ion"); + hoc_register_dparam_semantics(_mechtype, 1, "cal_ion"); + hoc_register_dparam_semantics(_mechtype, 2, "cal_ion"); + hoc_register_dparam_semantics(_mechtype, 3, "cal_ion"); + hoc_register_dparam_semantics(_mechtype, 4, "cvodeieq"); + hoc_register_cvode(_mechtype, _ode_count, _ode_map, _ode_spec, _ode_matsol); + hoc_register_tolerance(_mechtype, _hoc_state_tol, &_atollist); + hoc_register_var(hoc_scdoub, hoc_vdoub, hoc_intfunc); + ivoc_help("help ?1 cat32_ms /home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/cat32_ms.mod\n"); + hoc_register_limits(_mechtype, _hoc_parm_limits); + hoc_register_units(_mechtype, _hoc_parm_units); + } + +#define FARADAY _nrnunit_FARADAY[_nrnunit_use_legacy_] +static double _nrnunit_FARADAY[2] = {0x1.78e555060882cp+16, 96485.3}; /* 96485.3321233100141 */ + +#define R _nrnunit_R[_nrnunit_use_legacy_] +static double _nrnunit_R[2] = {0x1.0a1013e8990bep+3, 8.3145}; /* 8.3144626181532395 */ +static int _reset; +static char *modelname = "T-type calcium current (Cav3.2)"; + +static int error; +static int _ninits = 0; +static int _match_recurse=1; +static void _modl_cleanup(){ _match_recurse=1;} +static int rates(_threadargsproto_); + +static int _ode_spec1(_threadargsproto_); +/*static int _ode_matsol1(_threadargsproto_);*/ + static int _slist1[2], _dlist1[2]; + static int states(_threadargsproto_); + +/*CVODE*/ + static int _ode_spec1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) {int _reset = 0; { + rates ( _threadargs_ ) ; + Dm = ( minf - m ) / mtau * q ; + Dh = ( hinf - h ) / htau * q ; + } + return _reset; +} + static int _ode_matsol1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + rates ( _threadargs_ ) ; + Dm = Dm / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) )) ; + Dh = Dh / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / htau )*( q ) )) ; + return 0; +} + /*END CVODE*/ + static int states (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { { + rates ( _threadargs_ ) ; + m = m + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / mtau )*( q ))))*(- ( ( ( ( minf ) ) / mtau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) ) - m) ; + h = h + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / htau )*( q ))))*(- ( ( ( ( hinf ) ) / htau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / htau )*( q ) ) - h) ; + } + return 0; +} + +static int rates ( _threadargsproto_ ) { + minf = 1.0 / ( 1.0 + exp ( ( v - ( - 54.0 ) ) / ( - 7.8 ) ) ) ; + mtau = ( 2.1 + 23.0 / ( 1.0 + exp ( ( v - ( - 61.0 ) ) / 6.0 ) ) ) * 3.0 ; + hinf = 1.0 / ( 1.0 + exp ( ( v - ( - 64.2 ) ) / 8.8 ) ) ; + htau = 30.0 + 280.0 / ( 1.0 + exp ( ( v - ( - 52.0 ) ) / 7.0 ) ) ; + return 0; } + +static void _hoc_rates(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = 1.; + rates ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +double ghk ( _threadargsprotocomma_ double _lv , double _lci , double _lco ) { + double _lghk; + double _lz , _leci , _leco ; + _lz = ( 1e-3 ) * 2.0 * FARADAY * _lv / ( R * ( celsius + 273.15 ) ) ; + if ( _lz == 0.0 ) { + _lz = _lz + 1e-6 ; + } + _leco = _lco * ( _lz ) / ( exp ( _lz ) - 1.0 ) ; + _leci = _lci * ( - _lz ) / ( exp ( - _lz ) - 1.0 ) ; + _lghk = ( 1e-3 ) * 2.0 * FARADAY * ( _leci - _leco ) ; + +return _lghk; + } + +static void _hoc_ghk(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = ghk ( _p, _ppvar, _thread, _nt, *getarg(1) , *getarg(2) , *getarg(3) ); + hoc_retpushx(_r); +} + +static int _ode_count(int _type){ return 2;} + +static void _ode_spec(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + cali = _ion_cali; + calo = _ion_calo; + _ode_spec1 (_p, _ppvar, _thread, _nt); + }} + +static void _ode_map(int _ieq, double** _pv, double** _pvdot, double* _pp, Datum* _ppd, double* _atol, int _type) { + double* _p; Datum* _ppvar; + int _i; _p = _pp; _ppvar = _ppd; + _cvode_ieq = _ieq; + for (_i=0; _i < 2; ++_i) { + _pv[_i] = _pp + _slist1[_i]; _pvdot[_i] = _pp + _dlist1[_i]; + _cvode_abstol(_atollist, _atol, _i); + } + } + +static void _ode_matsol_instance1(_threadargsproto_) { + _ode_matsol1 (_p, _ppvar, _thread, _nt); + } + +static void _ode_matsol(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + cali = _ion_cali; + calo = _ion_calo; + _ode_matsol_instance1(_threadargs_); + }} + extern void nrn_update_ion_pointer(Symbol*, Datum*, int, int); + static void _update_ion_pointer(Datum* _ppvar) { + nrn_update_ion_pointer(_cal_sym, _ppvar, 0, 1); + nrn_update_ion_pointer(_cal_sym, _ppvar, 1, 2); + nrn_update_ion_pointer(_cal_sym, _ppvar, 2, 3); + nrn_update_ion_pointer(_cal_sym, _ppvar, 3, 4); + } + +static void initmodel(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + int _i; double _save;{ + h = h0; + m = m0; + { + rates ( _threadargs_ ) ; + m = minf ; + h = hinf ; + } + +} +} + +static void nrn_init(NrnThread* _nt, _Memb_list* _ml, int _type){ +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v = _v; + cali = _ion_cali; + calo = _ion_calo; + initmodel(_p, _ppvar, _thread, _nt); + } +} + +static double _nrn_current(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, double _v){double _current=0.;v=_v;{ { + ical = pbar * m * m * m * h * ghk ( _threadargscomma_ v , cali , calo ) ; + } + _current += ical; + +} return _current; +} + +static void nrn_cur(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; double _rhs, _v; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + cali = _ion_cali; + calo = _ion_calo; + _g = _nrn_current(_p, _ppvar, _thread, _nt, _v + .001); + { double _dical; + _dical = ical; + _rhs = _nrn_current(_p, _ppvar, _thread, _nt, _v); + _ion_dicaldv += (_dical - ical)/.001 ; + } + _g = (_g - _rhs)/.001; + _ion_ical += ical ; +#if CACHEVEC + if (use_cachevec) { + VEC_RHS(_ni[_iml]) -= _rhs; + }else +#endif + { + NODERHS(_nd) -= _rhs; + } + +} + +} + +static void nrn_jacob(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; +#if CACHEVEC + if (use_cachevec) { + VEC_D(_ni[_iml]) += _g; + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + NODED(_nd) += _g; + } + +} + +} + +static void nrn_state(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v = 0.0; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v=_v; +{ + cali = _ion_cali; + calo = _ion_calo; + { states(_p, _ppvar, _thread, _nt); + } }} + +} + +static void terminal(){} + +static void _initlists(){ + double _x; double* _p = &_x; + int _i; static int _first = 1; + if (!_first) return; + _slist1[0] = m_columnindex; _dlist1[0] = Dm_columnindex; + _slist1[1] = h_columnindex; _dlist1[1] = Dh_columnindex; +_first = 0; +} + +#if defined(__cplusplus) +} /* extern "C" */ +#endif + +#if NMODL_TEXT +static const char* nmodl_filename = "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/cat32_ms.mod"; +static const char* nmodl_file_text = + "TITLE T-type calcium current (Cav3.2)\n" + "\n" + "UNITS {\n" + " (mV) = (millivolt)\n" + " (mA) = (milliamp)\n" + " (S) = (siemens)\n" + " (molar) = (1/liter)\n" + " (mM) = (millimolar)\n" + " FARADAY = (faraday) (coulomb)\n" + " R = (k-mole) (joule/degC)\n" + "}\n" + "\n" + "NEURON {\n" + " SUFFIX cat32_ms\n" + " USEION cal READ cali, calo WRITE ical VALENCE 2\n" + " RANGE pbar, ical\n" + "}\n" + "\n" + "PARAMETER {\n" + " pbar = 0.0 (cm/s)\n" + " :q = 1 : room temperature 21 C\n" + " q = 3 : body temperature 35 C\n" + "} \n" + "\n" + "ASSIGNED { \n" + " v (mV)\n" + " ical (mA/cm2)\n" + " ecal (mV)\n" + " celsius (degC)\n" + " cali (mM)\n" + " calo (mM)\n" + " minf\n" + " mtau (ms)\n" + " hinf\n" + " htau (ms)\n" + "}\n" + "\n" + "STATE { m h }\n" + "\n" + "BREAKPOINT {\n" + " SOLVE states METHOD cnexp\n" + " ical = pbar*m*m*m*h*ghk(v, cali, calo)\n" + "}\n" + "\n" + "INITIAL {\n" + " rates()\n" + " m = minf\n" + " h = hinf\n" + "}\n" + "\n" + "DERIVATIVE states { \n" + " rates()\n" + " m' = (minf-m)/mtau*q\n" + " h' = (hinf-h)/htau*q\n" + "}\n" + "\n" + "PROCEDURE rates() {\n" + " UNITSOFF\n" + " minf = 1/(1+exp((v-(-54))/(-7.8)))\n" + " mtau = (2.1+23/(1+exp((v-(-61))/6)))*3\n" + " hinf = 1/(1+exp((v-(-64.2))/8.8))\n" + " htau = 30+280/(1+exp((v-(-52))/7))\n" + " UNITSON\n" + "}\n" + "\n" + "FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) {\n" + " LOCAL z, eci, eco\n" + " z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15))\n" + " if(z == 0) {\n" + " z = z+1e-6\n" + " }\n" + " eco = co*(z)/(exp(z)-1)\n" + " eci = ci*(-z)/(exp(-z)-1)\n" + " ghk = (1e-3)*2*FARADAY*(eci-eco)\n" + "}\n" + "\n" + "COMMENT\n" + "\n" + "Rat Cav3.2 channels were isolated and transfection of human embryonic\n" + "kidney cells was performed [1]. Electrophysiological recordings were\n" + "done in 21 C.\n" + "\n" + "NEURON model by Alexander Kozlov . Kinetics of m3h\n" + "type was used [2-4]. Activation time constant was scaled up accordingly.\n" + "\n" + "[1] Iftinca M, McKay BE, Snutch TP, McRory JE, Turner RW, Zamponi\n" + "GW (2006) Temperature dependence of T-type calcium channel\n" + "gating. Neuroscience 142(4):1031-42.\n" + "\n" + "[2] Crunelli V, Toth TI, Cope DW, Blethyn K, Hughes SW (2005) The\n" + "'window' T-type calcium current in brain dynamics of different behavioural\n" + "states. J Physiol 562(Pt 1):121-9.\n" + "\n" + "[3] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M,\n" + "O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions\n" + "and entrainment to oscillations in a computational model of the nucleus\n" + "accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95.\n" + "\n" + "[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of\n" + "spike timing-dependent calcium influx during corticostriatal upstates. J\n" + "Neurophysiol 110(7):1631-45.\n" + "\n" + "ENDCOMMENT\n" + ; +#endif diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/cat32_ms.o b/examples/example_models/str-dspn-model1/mechanisms/x86_64/cat32_ms.o new file mode 100644 index 00000000..c44c929e Binary files /dev/null and b/examples/example_models/str-dspn-model1/mechanisms/x86_64/cat32_ms.o differ diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/cat33_ms.c b/examples/example_models/str-dspn-model1/mechanisms/x86_64/cat33_ms.c new file mode 100644 index 00000000..9151e478 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/x86_64/cat33_ms.c @@ -0,0 +1,649 @@ +/* Created by Language version: 7.7.0 */ +/* VECTORIZED */ +#define NRN_VECTORIZED 1 +#include +#include +#include +#include "mech_api.h" +#undef PI +#define nil 0 +#include "md1redef.h" +#include "section.h" +#include "nrniv_mf.h" +#include "md2redef.h" + +#if METHOD3 +extern int _method3; +#endif + +#if !NRNGPU +#undef exp +#define exp hoc_Exp +extern double hoc_Exp(double); +#endif + +#define nrn_init _nrn_init__cat33_ms +#define _nrn_initial _nrn_initial__cat33_ms +#define nrn_cur _nrn_cur__cat33_ms +#define _nrn_current _nrn_current__cat33_ms +#define nrn_jacob _nrn_jacob__cat33_ms +#define nrn_state _nrn_state__cat33_ms +#define _net_receive _net_receive__cat33_ms +#define rates rates__cat33_ms +#define states states__cat33_ms + +#define _threadargscomma_ _p, _ppvar, _thread, _nt, +#define _threadargsprotocomma_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, +#define _threadargs_ _p, _ppvar, _thread, _nt +#define _threadargsproto_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt + /*SUPPRESS 761*/ + /*SUPPRESS 762*/ + /*SUPPRESS 763*/ + /*SUPPRESS 765*/ + extern double *getarg(); + /* Thread safe. No static _p or _ppvar. */ + +#define t _nt->_t +#define dt _nt->_dt +#define pbar _p[0] +#define pbar_columnindex 0 +#define ical _p[1] +#define ical_columnindex 1 +#define m _p[2] +#define m_columnindex 2 +#define h _p[3] +#define h_columnindex 3 +#define ecal _p[4] +#define ecal_columnindex 4 +#define cali _p[5] +#define cali_columnindex 5 +#define calo _p[6] +#define calo_columnindex 6 +#define minf _p[7] +#define minf_columnindex 7 +#define mtau _p[8] +#define mtau_columnindex 8 +#define hinf _p[9] +#define hinf_columnindex 9 +#define htau _p[10] +#define htau_columnindex 10 +#define Dm _p[11] +#define Dm_columnindex 11 +#define Dh _p[12] +#define Dh_columnindex 12 +#define v _p[13] +#define v_columnindex 13 +#define _g _p[14] +#define _g_columnindex 14 +#define _ion_cali *_ppvar[0]._pval +#define _ion_calo *_ppvar[1]._pval +#define _ion_ical *_ppvar[2]._pval +#define _ion_dicaldv *_ppvar[3]._pval + +#if MAC +#if !defined(v) +#define v _mlhv +#endif +#if !defined(h) +#define h _mlhh +#endif +#endif + +#if defined(__cplusplus) +extern "C" { +#endif + static int hoc_nrnpointerindex = -1; + static Datum* _extcall_thread; + static Prop* _extcall_prop; + /* external NEURON variables */ + extern double celsius; + /* declaration of user functions */ + static void _hoc_ghk(void); + static void _hoc_rates(void); + static int _mechtype; +extern void _nrn_cacheloop_reg(int, int); +extern void hoc_register_prop_size(int, int, int); +extern void hoc_register_limits(int, HocParmLimits*); +extern void hoc_register_units(int, HocParmUnits*); +extern void nrn_promote(Prop*, int, int); +extern Memb_func* memb_func; + +#define NMODL_TEXT 1 +#if NMODL_TEXT +static const char* nmodl_file_text; +static const char* nmodl_filename; +extern void hoc_reg_nmodl_text(int, const char*); +extern void hoc_reg_nmodl_filename(int, const char*); +#endif + + extern void _nrn_setdata_reg(int, void(*)(Prop*)); + static void _setdata(Prop* _prop) { + _extcall_prop = _prop; + } + static void _hoc_setdata() { + Prop *_prop, *hoc_getdata_range(int); + _prop = hoc_getdata_range(_mechtype); + _setdata(_prop); + hoc_retpushx(1.); +} + /* connect user functions to hoc names */ + static VoidFunc hoc_intfunc[] = { + "setdata_cat33_ms", _hoc_setdata, + "ghk_cat33_ms", _hoc_ghk, + "rates_cat33_ms", _hoc_rates, + 0, 0 +}; +#define ghk ghk_cat33_ms + extern double ghk( _threadargsprotocomma_ double , double , double ); + /* declare global and static user variables */ +#define q q_cat33_ms + double q = 3; + /* some parameters have upper and lower limits */ + static HocParmLimits _hoc_parm_limits[] = { + 0,0,0 +}; + static HocParmUnits _hoc_parm_units[] = { + "pbar_cat33_ms", "cm/s", + "ical_cat33_ms", "mA/cm2", + 0,0 +}; + static double delta_t = 0.01; + static double h0 = 0; + static double m0 = 0; + /* connect global user variables to hoc */ + static DoubScal hoc_scdoub[] = { + "q_cat33_ms", &q_cat33_ms, + 0,0 +}; + static DoubVec hoc_vdoub[] = { + 0,0,0 +}; + static double _sav_indep; + static void nrn_alloc(Prop*); +static void nrn_init(NrnThread*, _Memb_list*, int); +static void nrn_state(NrnThread*, _Memb_list*, int); + static void nrn_cur(NrnThread*, _Memb_list*, int); +static void nrn_jacob(NrnThread*, _Memb_list*, int); + +static int _ode_count(int); +static void _ode_map(int, double**, double**, double*, Datum*, double*, int); +static void _ode_spec(NrnThread*, _Memb_list*, int); +static void _ode_matsol(NrnThread*, _Memb_list*, int); + +#define _cvode_ieq _ppvar[4]._i + static void _ode_matsol_instance1(_threadargsproto_); + /* connect range variables in _p that hoc is supposed to know about */ + static const char *_mechanism[] = { + "7.7.0", +"cat33_ms", + "pbar_cat33_ms", + 0, + "ical_cat33_ms", + 0, + "m_cat33_ms", + "h_cat33_ms", + 0, + 0}; + static Symbol* _cal_sym; + +extern Prop* need_memb(Symbol*); + +static void nrn_alloc(Prop* _prop) { + Prop *prop_ion; + double *_p; Datum *_ppvar; + _p = nrn_prop_data_alloc(_mechtype, 15, _prop); + /*initialize range parameters*/ + pbar = 0; + _prop->param = _p; + _prop->param_size = 15; + _ppvar = nrn_prop_datum_alloc(_mechtype, 5, _prop); + _prop->dparam = _ppvar; + /*connect ionic variables to this model*/ + prop_ion = need_memb(_cal_sym); + nrn_promote(prop_ion, 1, 0); + _ppvar[0]._pval = &prop_ion->param[1]; /* cali */ + _ppvar[1]._pval = &prop_ion->param[2]; /* calo */ + _ppvar[2]._pval = &prop_ion->param[3]; /* ical */ + _ppvar[3]._pval = &prop_ion->param[4]; /* _ion_dicaldv */ + +} + static void _initlists(); + /* some states have an absolute tolerance */ + static Symbol** _atollist; + static HocStateTolerance _hoc_state_tol[] = { + 0,0 +}; + static void _update_ion_pointer(Datum*); + extern Symbol* hoc_lookup(const char*); +extern void _nrn_thread_reg(int, int, void(*)(Datum*)); +extern void _nrn_thread_table_reg(int, void(*)(double*, Datum*, Datum*, NrnThread*, int)); +extern void hoc_register_tolerance(int, HocStateTolerance*, Symbol***); +extern void _cvode_abstol( Symbol**, double*, int); + + void _cat33_ms_reg() { + int _vectorized = 1; + _initlists(); + ion_reg("cal", 2.0); + _cal_sym = hoc_lookup("cal_ion"); + register_mech(_mechanism, nrn_alloc,nrn_cur, nrn_jacob, nrn_state, nrn_init, hoc_nrnpointerindex, 1); + _mechtype = nrn_get_mechtype(_mechanism[1]); + _nrn_setdata_reg(_mechtype, _setdata); + _nrn_thread_reg(_mechtype, 2, _update_ion_pointer); + #if NMODL_TEXT + hoc_reg_nmodl_text(_mechtype, nmodl_file_text); + hoc_reg_nmodl_filename(_mechtype, nmodl_filename); +#endif + hoc_register_prop_size(_mechtype, 15, 5); + hoc_register_dparam_semantics(_mechtype, 0, "cal_ion"); + hoc_register_dparam_semantics(_mechtype, 1, "cal_ion"); + hoc_register_dparam_semantics(_mechtype, 2, "cal_ion"); + hoc_register_dparam_semantics(_mechtype, 3, "cal_ion"); + hoc_register_dparam_semantics(_mechtype, 4, "cvodeieq"); + hoc_register_cvode(_mechtype, _ode_count, _ode_map, _ode_spec, _ode_matsol); + hoc_register_tolerance(_mechtype, _hoc_state_tol, &_atollist); + hoc_register_var(hoc_scdoub, hoc_vdoub, hoc_intfunc); + ivoc_help("help ?1 cat33_ms /home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/cat33_ms.mod\n"); + hoc_register_limits(_mechtype, _hoc_parm_limits); + hoc_register_units(_mechtype, _hoc_parm_units); + } + +#define FARADAY _nrnunit_FARADAY[_nrnunit_use_legacy_] +static double _nrnunit_FARADAY[2] = {0x1.78e555060882cp+16, 96485.3}; /* 96485.3321233100141 */ + +#define R _nrnunit_R[_nrnunit_use_legacy_] +static double _nrnunit_R[2] = {0x1.0a1013e8990bep+3, 8.3145}; /* 8.3144626181532395 */ +static int _reset; +static char *modelname = "T-type calcium current (Cav3.3)"; + +static int error; +static int _ninits = 0; +static int _match_recurse=1; +static void _modl_cleanup(){ _match_recurse=1;} +static int rates(_threadargsproto_); + +static int _ode_spec1(_threadargsproto_); +/*static int _ode_matsol1(_threadargsproto_);*/ + static int _slist1[2], _dlist1[2]; + static int states(_threadargsproto_); + +/*CVODE*/ + static int _ode_spec1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) {int _reset = 0; { + rates ( _threadargs_ ) ; + Dm = ( minf - m ) / mtau * q ; + Dh = ( hinf - h ) / htau * q ; + } + return _reset; +} + static int _ode_matsol1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + rates ( _threadargs_ ) ; + Dm = Dm / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) )) ; + Dh = Dh / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / htau )*( q ) )) ; + return 0; +} + /*END CVODE*/ + static int states (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { { + rates ( _threadargs_ ) ; + m = m + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / mtau )*( q ))))*(- ( ( ( ( minf ) ) / mtau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) ) - m) ; + h = h + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / htau )*( q ))))*(- ( ( ( ( hinf ) ) / htau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / htau )*( q ) ) - h) ; + } + return 0; +} + +static int rates ( _threadargsproto_ ) { + minf = 1.0 / ( 1.0 + exp ( ( v - ( - 81.0 ) ) / ( - 5.8 ) ) ) ; + mtau = ( 2.3 + 20.0 / ( 1.0 + exp ( ( v - ( - 60.0 ) ) / 9.0 ) ) ) * 3.0 ; + hinf = 1.0 / ( 1.0 + exp ( ( v - ( - 78.3 ) ) / 6.5 ) ) ; + htau = 125.0 + 140.0 / ( 1.0 + exp ( ( v - ( - 60.0 ) ) / 3.0 ) ) ; + return 0; } + +static void _hoc_rates(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = 1.; + rates ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +double ghk ( _threadargsprotocomma_ double _lv , double _lci , double _lco ) { + double _lghk; + double _lz , _leci , _leco ; + _lz = ( 1e-3 ) * 2.0 * FARADAY * _lv / ( R * ( celsius + 273.15 ) ) ; + if ( _lz == 0.0 ) { + _lz = _lz + 1e-6 ; + } + _leco = _lco * ( _lz ) / ( exp ( _lz ) - 1.0 ) ; + _leci = _lci * ( - _lz ) / ( exp ( - _lz ) - 1.0 ) ; + _lghk = ( 1e-3 ) * 2.0 * FARADAY * ( _leci - _leco ) ; + +return _lghk; + } + +static void _hoc_ghk(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = ghk ( _p, _ppvar, _thread, _nt, *getarg(1) , *getarg(2) , *getarg(3) ); + hoc_retpushx(_r); +} + +static int _ode_count(int _type){ return 2;} + +static void _ode_spec(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + cali = _ion_cali; + calo = _ion_calo; + _ode_spec1 (_p, _ppvar, _thread, _nt); + }} + +static void _ode_map(int _ieq, double** _pv, double** _pvdot, double* _pp, Datum* _ppd, double* _atol, int _type) { + double* _p; Datum* _ppvar; + int _i; _p = _pp; _ppvar = _ppd; + _cvode_ieq = _ieq; + for (_i=0; _i < 2; ++_i) { + _pv[_i] = _pp + _slist1[_i]; _pvdot[_i] = _pp + _dlist1[_i]; + _cvode_abstol(_atollist, _atol, _i); + } + } + +static void _ode_matsol_instance1(_threadargsproto_) { + _ode_matsol1 (_p, _ppvar, _thread, _nt); + } + +static void _ode_matsol(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + cali = _ion_cali; + calo = _ion_calo; + _ode_matsol_instance1(_threadargs_); + }} + extern void nrn_update_ion_pointer(Symbol*, Datum*, int, int); + static void _update_ion_pointer(Datum* _ppvar) { + nrn_update_ion_pointer(_cal_sym, _ppvar, 0, 1); + nrn_update_ion_pointer(_cal_sym, _ppvar, 1, 2); + nrn_update_ion_pointer(_cal_sym, _ppvar, 2, 3); + nrn_update_ion_pointer(_cal_sym, _ppvar, 3, 4); + } + +static void initmodel(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + int _i; double _save;{ + h = h0; + m = m0; + { + rates ( _threadargs_ ) ; + m = minf ; + h = hinf ; + } + +} +} + +static void nrn_init(NrnThread* _nt, _Memb_list* _ml, int _type){ +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v = _v; + cali = _ion_cali; + calo = _ion_calo; + initmodel(_p, _ppvar, _thread, _nt); + } +} + +static double _nrn_current(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, double _v){double _current=0.;v=_v;{ { + ical = pbar * m * m * m * h * ghk ( _threadargscomma_ v , cali , calo ) ; + } + _current += ical; + +} return _current; +} + +static void nrn_cur(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; double _rhs, _v; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + cali = _ion_cali; + calo = _ion_calo; + _g = _nrn_current(_p, _ppvar, _thread, _nt, _v + .001); + { double _dical; + _dical = ical; + _rhs = _nrn_current(_p, _ppvar, _thread, _nt, _v); + _ion_dicaldv += (_dical - ical)/.001 ; + } + _g = (_g - _rhs)/.001; + _ion_ical += ical ; +#if CACHEVEC + if (use_cachevec) { + VEC_RHS(_ni[_iml]) -= _rhs; + }else +#endif + { + NODERHS(_nd) -= _rhs; + } + +} + +} + +static void nrn_jacob(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; +#if CACHEVEC + if (use_cachevec) { + VEC_D(_ni[_iml]) += _g; + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + NODED(_nd) += _g; + } + +} + +} + +static void nrn_state(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v = 0.0; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v=_v; +{ + cali = _ion_cali; + calo = _ion_calo; + { states(_p, _ppvar, _thread, _nt); + } }} + +} + +static void terminal(){} + +static void _initlists(){ + double _x; double* _p = &_x; + int _i; static int _first = 1; + if (!_first) return; + _slist1[0] = m_columnindex; _dlist1[0] = Dm_columnindex; + _slist1[1] = h_columnindex; _dlist1[1] = Dh_columnindex; +_first = 0; +} + +#if defined(__cplusplus) +} /* extern "C" */ +#endif + +#if NMODL_TEXT +static const char* nmodl_filename = "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/cat33_ms.mod"; +static const char* nmodl_file_text = + "TITLE T-type calcium current (Cav3.3)\n" + "\n" + "UNITS {\n" + " (mV) = (millivolt)\n" + " (mA) = (milliamp)\n" + " (S) = (siemens)\n" + " (molar) = (1/liter)\n" + " (mM) = (millimolar)\n" + " FARADAY = (faraday) (coulomb)\n" + " R = (k-mole) (joule/degC)\n" + "}\n" + "\n" + "NEURON {\n" + " SUFFIX cat33_ms\n" + " USEION cal READ cali, calo WRITE ical VALENCE 2\n" + " RANGE pbar, ical\n" + "}\n" + "\n" + "PARAMETER {\n" + " pbar = 0.0 (cm/s)\n" + " :q = 1 : room temperature 21 C\n" + " q = 3 : body temperature 35 C\n" + "} \n" + "\n" + "ASSIGNED { \n" + " v (mV)\n" + " ical (mA/cm2)\n" + " ecal (mV)\n" + " celsius (degC)\n" + " cali (mM)\n" + " calo (mM)\n" + " minf\n" + " mtau (ms)\n" + " hinf\n" + " htau (ms)\n" + "}\n" + "\n" + "STATE { m h }\n" + "\n" + "BREAKPOINT {\n" + " SOLVE states METHOD cnexp\n" + " ical = pbar*m*m*m*h*ghk(v, cali, calo)\n" + "}\n" + "\n" + "INITIAL {\n" + " rates()\n" + " m = minf\n" + " h = hinf\n" + "}\n" + "\n" + "DERIVATIVE states { \n" + " rates()\n" + " m' = (minf-m)/mtau*q\n" + " h' = (hinf-h)/htau*q\n" + "}\n" + "\n" + "PROCEDURE rates() {\n" + " UNITSOFF\n" + " minf = 1/(1+exp((v-(-81))/(-5.8)))\n" + " mtau = (2.3+20/(1+exp((v-(-60))/9)))*3\n" + " hinf = 1/(1+exp((v-(-78.3))/6.5))\n" + " htau = 125+140/(1+exp((v-(-60))/3))\n" + " UNITSON\n" + "}\n" + "\n" + "FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) {\n" + " LOCAL z, eci, eco\n" + " z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15))\n" + " if(z == 0) {\n" + " z = z+1e-6\n" + " }\n" + " eco = co*(z)/(exp(z)-1)\n" + " eci = ci*(-z)/(exp(-z)-1)\n" + " ghk = (1e-3)*2*FARADAY*(eci-eco)\n" + "}\n" + "\n" + "COMMENT\n" + "\n" + "Rat Cav3.2 channels were isolated and transfection of human embryonic\n" + "kidney cells was performed [1]. Electrophysiological recordings were\n" + "done in 21 C.\n" + "\n" + "NEURON model by Alexander Kozlov . Kinetics of m3h\n" + "type was used [2-4]. Activation time constant was scaled up accordingly.\n" + "\n" + "[1] Iftinca M, McKay BE, Snutch TP, McRory JE, Turner RW, Zamponi\n" + "GW (2006) Temperature dependence of T-type calcium channel\n" + "gating. Neuroscience 142(4):1031-42.\n" + "\n" + "[2] Crunelli V, Toth TI, Cope DW, Blethyn K, Hughes SW (2005) The\n" + "'window' T-type calcium current in brain dynamics of different behavioural\n" + "states. J Physiol 562(Pt 1):121-9.\n" + "\n" + "[3] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M,\n" + "O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions\n" + "and entrainment to oscillations in a computational model of the nucleus\n" + "accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95.\n" + "\n" + "[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of\n" + "spike timing-dependent calcium influx during corticostriatal upstates. J\n" + "Neurophysiol 110(7):1631-45.\n" + "\n" + "ENDCOMMENT\n" + ; +#endif diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/cat33_ms.o b/examples/example_models/str-dspn-model1/mechanisms/x86_64/cat33_ms.o new file mode 100644 index 00000000..ed878d15 Binary files /dev/null and b/examples/example_models/str-dspn-model1/mechanisms/x86_64/cat33_ms.o differ diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/kaf_ms.c b/examples/example_models/str-dspn-model1/mechanisms/x86_64/kaf_ms.c new file mode 100644 index 00000000..b83bae5b --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/x86_64/kaf_ms.c @@ -0,0 +1,745 @@ +/* Created by Language version: 7.7.0 */ +/* VECTORIZED */ +#define NRN_VECTORIZED 1 +#include +#include +#include +#include "mech_api.h" +#undef PI +#define nil 0 +#include "md1redef.h" +#include "section.h" +#include "nrniv_mf.h" +#include "md2redef.h" + +#if METHOD3 +extern int _method3; +#endif + +#if !NRNGPU +#undef exp +#define exp hoc_Exp +extern double hoc_Exp(double); +#endif + +#define nrn_init _nrn_init__kaf_ms +#define _nrn_initial _nrn_initial__kaf_ms +#define nrn_cur _nrn_cur__kaf_ms +#define _nrn_current _nrn_current__kaf_ms +#define nrn_jacob _nrn_jacob__kaf_ms +#define nrn_state _nrn_state__kaf_ms +#define _net_receive _net_receive__kaf_ms +#define rates rates__kaf_ms +#define states states__kaf_ms + +#define _threadargscomma_ _p, _ppvar, _thread, _nt, +#define _threadargsprotocomma_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, +#define _threadargs_ _p, _ppvar, _thread, _nt +#define _threadargsproto_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt + /*SUPPRESS 761*/ + /*SUPPRESS 762*/ + /*SUPPRESS 763*/ + /*SUPPRESS 765*/ + extern double *getarg(); + /* Thread safe. No static _p or _ppvar. */ + +#define t _nt->_t +#define dt _nt->_dt +#define gbar _p[0] +#define gbar_columnindex 0 +#define q _p[1] +#define q_columnindex 1 +#define modDA _p[2] +#define modDA_columnindex 2 +#define maxModDA _p[3] +#define maxModDA_columnindex 3 +#define levelDA _p[4] +#define levelDA_columnindex 4 +#define modShift _p[5] +#define modShift_columnindex 5 +#define modACh _p[6] +#define modACh_columnindex 6 +#define maxModACh _p[7] +#define maxModACh_columnindex 7 +#define levelACh _p[8] +#define levelACh_columnindex 8 +#define ik _p[9] +#define ik_columnindex 9 +#define gk _p[10] +#define gk_columnindex 10 +#define m _p[11] +#define m_columnindex 11 +#define h _p[12] +#define h_columnindex 12 +#define ek _p[13] +#define ek_columnindex 13 +#define minf _p[14] +#define minf_columnindex 14 +#define mtau _p[15] +#define mtau_columnindex 15 +#define hinf _p[16] +#define hinf_columnindex 16 +#define htau _p[17] +#define htau_columnindex 17 +#define Dm _p[18] +#define Dm_columnindex 18 +#define Dh _p[19] +#define Dh_columnindex 19 +#define v _p[20] +#define v_columnindex 20 +#define _g _p[21] +#define _g_columnindex 21 +#define _ion_ek *_ppvar[0]._pval +#define _ion_ik *_ppvar[1]._pval +#define _ion_dikdv *_ppvar[2]._pval + +#if MAC +#if !defined(v) +#define v _mlhv +#endif +#if !defined(h) +#define h _mlhh +#endif +#endif + +#if defined(__cplusplus) +extern "C" { +#endif + static int hoc_nrnpointerindex = -1; + static Datum* _extcall_thread; + static Prop* _extcall_prop; + /* external NEURON variables */ + /* declaration of user functions */ + static void _hoc_modulationACh(void); + static void _hoc_modulationDA(void); + static void _hoc_rates(void); + static int _mechtype; +extern void _nrn_cacheloop_reg(int, int); +extern void hoc_register_prop_size(int, int, int); +extern void hoc_register_limits(int, HocParmLimits*); +extern void hoc_register_units(int, HocParmUnits*); +extern void nrn_promote(Prop*, int, int); +extern Memb_func* memb_func; + +#define NMODL_TEXT 1 +#if NMODL_TEXT +static const char* nmodl_file_text; +static const char* nmodl_filename; +extern void hoc_reg_nmodl_text(int, const char*); +extern void hoc_reg_nmodl_filename(int, const char*); +#endif + + extern void _nrn_setdata_reg(int, void(*)(Prop*)); + static void _setdata(Prop* _prop) { + _extcall_prop = _prop; + } + static void _hoc_setdata() { + Prop *_prop, *hoc_getdata_range(int); + _prop = hoc_getdata_range(_mechtype); + _setdata(_prop); + hoc_retpushx(1.); +} + /* connect user functions to hoc names */ + static VoidFunc hoc_intfunc[] = { + "setdata_kaf_ms", _hoc_setdata, + "modulationACh_kaf_ms", _hoc_modulationACh, + "modulationDA_kaf_ms", _hoc_modulationDA, + "rates_kaf_ms", _hoc_rates, + 0, 0 +}; +#define modulationACh modulationACh_kaf_ms +#define modulationDA modulationDA_kaf_ms + extern double modulationACh( _threadargsproto_ ); + extern double modulationDA( _threadargsproto_ ); + /* declare global and static user variables */ + /* some parameters have upper and lower limits */ + static HocParmLimits _hoc_parm_limits[] = { + 0,0,0 +}; + static HocParmUnits _hoc_parm_units[] = { + "gbar_kaf_ms", "S/cm2", + "ik_kaf_ms", "mA/cm2", + "gk_kaf_ms", "S/cm2", + 0,0 +}; + static double delta_t = 0.01; + static double h0 = 0; + static double m0 = 0; + /* connect global user variables to hoc */ + static DoubScal hoc_scdoub[] = { + 0,0 +}; + static DoubVec hoc_vdoub[] = { + 0,0,0 +}; + static double _sav_indep; + static void nrn_alloc(Prop*); +static void nrn_init(NrnThread*, _Memb_list*, int); +static void nrn_state(NrnThread*, _Memb_list*, int); + static void nrn_cur(NrnThread*, _Memb_list*, int); +static void nrn_jacob(NrnThread*, _Memb_list*, int); + +static int _ode_count(int); +static void _ode_map(int, double**, double**, double*, Datum*, double*, int); +static void _ode_spec(NrnThread*, _Memb_list*, int); +static void _ode_matsol(NrnThread*, _Memb_list*, int); + +#define _cvode_ieq _ppvar[3]._i + static void _ode_matsol_instance1(_threadargsproto_); + /* connect range variables in _p that hoc is supposed to know about */ + static const char *_mechanism[] = { + "7.7.0", +"kaf_ms", + "gbar_kaf_ms", + "q_kaf_ms", + "modDA_kaf_ms", + "maxModDA_kaf_ms", + "levelDA_kaf_ms", + "modShift_kaf_ms", + "modACh_kaf_ms", + "maxModACh_kaf_ms", + "levelACh_kaf_ms", + 0, + "ik_kaf_ms", + "gk_kaf_ms", + 0, + "m_kaf_ms", + "h_kaf_ms", + 0, + 0}; + static Symbol* _k_sym; + +extern Prop* need_memb(Symbol*); + +static void nrn_alloc(Prop* _prop) { + Prop *prop_ion; + double *_p; Datum *_ppvar; + _p = nrn_prop_data_alloc(_mechtype, 22, _prop); + /*initialize range parameters*/ + gbar = 0; + q = 1; + modDA = 0; + maxModDA = 1; + levelDA = 0; + modShift = 0; + modACh = 0; + maxModACh = 1; + levelACh = 0; + _prop->param = _p; + _prop->param_size = 22; + _ppvar = nrn_prop_datum_alloc(_mechtype, 4, _prop); + _prop->dparam = _ppvar; + /*connect ionic variables to this model*/ + prop_ion = need_memb(_k_sym); + nrn_promote(prop_ion, 0, 1); + _ppvar[0]._pval = &prop_ion->param[0]; /* ek */ + _ppvar[1]._pval = &prop_ion->param[3]; /* ik */ + _ppvar[2]._pval = &prop_ion->param[4]; /* _ion_dikdv */ + +} + static void _initlists(); + /* some states have an absolute tolerance */ + static Symbol** _atollist; + static HocStateTolerance _hoc_state_tol[] = { + 0,0 +}; + static void _update_ion_pointer(Datum*); + extern Symbol* hoc_lookup(const char*); +extern void _nrn_thread_reg(int, int, void(*)(Datum*)); +extern void _nrn_thread_table_reg(int, void(*)(double*, Datum*, Datum*, NrnThread*, int)); +extern void hoc_register_tolerance(int, HocStateTolerance*, Symbol***); +extern void _cvode_abstol( Symbol**, double*, int); + + void _kaf_ms_reg() { + int _vectorized = 1; + _initlists(); + ion_reg("k", -10000.); + _k_sym = hoc_lookup("k_ion"); + register_mech(_mechanism, nrn_alloc,nrn_cur, nrn_jacob, nrn_state, nrn_init, hoc_nrnpointerindex, 1); + _mechtype = nrn_get_mechtype(_mechanism[1]); + _nrn_setdata_reg(_mechtype, _setdata); + _nrn_thread_reg(_mechtype, 2, _update_ion_pointer); + #if NMODL_TEXT + hoc_reg_nmodl_text(_mechtype, nmodl_file_text); + hoc_reg_nmodl_filename(_mechtype, nmodl_filename); +#endif + hoc_register_prop_size(_mechtype, 22, 4); + hoc_register_dparam_semantics(_mechtype, 0, "k_ion"); + hoc_register_dparam_semantics(_mechtype, 1, "k_ion"); + hoc_register_dparam_semantics(_mechtype, 2, "k_ion"); + hoc_register_dparam_semantics(_mechtype, 3, "cvodeieq"); + hoc_register_cvode(_mechtype, _ode_count, _ode_map, _ode_spec, _ode_matsol); + hoc_register_tolerance(_mechtype, _hoc_state_tol, &_atollist); + hoc_register_var(hoc_scdoub, hoc_vdoub, hoc_intfunc); + ivoc_help("help ?1 kaf_ms /home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/kaf_ms.mod\n"); + hoc_register_limits(_mechtype, _hoc_parm_limits); + hoc_register_units(_mechtype, _hoc_parm_units); + } +static int _reset; +static char *modelname = "Fast A-type potassium current (Kv4.2)"; + +static int error; +static int _ninits = 0; +static int _match_recurse=1; +static void _modl_cleanup(){ _match_recurse=1;} +static int rates(_threadargsproto_); + +static int _ode_spec1(_threadargsproto_); +/*static int _ode_matsol1(_threadargsproto_);*/ + static int _slist1[2], _dlist1[2]; + static int states(_threadargsproto_); + +/*CVODE*/ + static int _ode_spec1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) {int _reset = 0; { + rates ( _threadargs_ ) ; + Dm = ( minf - m ) / mtau * q ; + Dh = ( hinf - h ) / htau * q ; + } + return _reset; +} + static int _ode_matsol1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + rates ( _threadargs_ ) ; + Dm = Dm / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) )) ; + Dh = Dh / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / htau )*( q ) )) ; + return 0; +} + /*END CVODE*/ + static int states (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { { + rates ( _threadargs_ ) ; + m = m + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / mtau )*( q ))))*(- ( ( ( ( minf ) ) / mtau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) ) - m) ; + h = h + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / htau )*( q ))))*(- ( ( ( ( hinf ) ) / htau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / htau )*( q ) ) - h) ; + } + return 0; +} + +static int rates ( _threadargsproto_ ) { + minf = 1.0 / ( 1.0 + exp ( ( v - ( - 10.0 + modShift ) ) / ( - 17.7 ) ) ) ; + mtau = 0.9 + 1.1 / ( 1.0 + exp ( ( v - ( - 30.0 ) ) / 10.0 ) ) ; + hinf = 1.0 / ( 1.0 + exp ( ( v - ( - 75.6 ) ) / 11.8 ) ) ; + htau = 14.0 ; + return 0; } + +static void _hoc_rates(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = 1.; + rates ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +double modulationDA ( _threadargsproto_ ) { + double _lmodulationDA; + _lmodulationDA = 1.0 + modDA * ( maxModDA - 1.0 ) * levelDA ; + +return _lmodulationDA; + } + +static void _hoc_modulationDA(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = modulationDA ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +double modulationACh ( _threadargsproto_ ) { + double _lmodulationACh; + _lmodulationACh = 1.0 + modACh * ( maxModACh - 1.0 ) * levelACh ; + +return _lmodulationACh; + } + +static void _hoc_modulationACh(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = modulationACh ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +static int _ode_count(int _type){ return 2;} + +static void _ode_spec(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + ek = _ion_ek; + _ode_spec1 (_p, _ppvar, _thread, _nt); + }} + +static void _ode_map(int _ieq, double** _pv, double** _pvdot, double* _pp, Datum* _ppd, double* _atol, int _type) { + double* _p; Datum* _ppvar; + int _i; _p = _pp; _ppvar = _ppd; + _cvode_ieq = _ieq; + for (_i=0; _i < 2; ++_i) { + _pv[_i] = _pp + _slist1[_i]; _pvdot[_i] = _pp + _dlist1[_i]; + _cvode_abstol(_atollist, _atol, _i); + } + } + +static void _ode_matsol_instance1(_threadargsproto_) { + _ode_matsol1 (_p, _ppvar, _thread, _nt); + } + +static void _ode_matsol(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + ek = _ion_ek; + _ode_matsol_instance1(_threadargs_); + }} + extern void nrn_update_ion_pointer(Symbol*, Datum*, int, int); + static void _update_ion_pointer(Datum* _ppvar) { + nrn_update_ion_pointer(_k_sym, _ppvar, 0, 0); + nrn_update_ion_pointer(_k_sym, _ppvar, 1, 3); + nrn_update_ion_pointer(_k_sym, _ppvar, 2, 4); + } + +static void initmodel(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + int _i; double _save;{ + h = h0; + m = m0; + { + rates ( _threadargs_ ) ; + m = minf ; + h = hinf ; + } + +} +} + +static void nrn_init(NrnThread* _nt, _Memb_list* _ml, int _type){ +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v = _v; + ek = _ion_ek; + initmodel(_p, _ppvar, _thread, _nt); + } +} + +static double _nrn_current(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, double _v){double _current=0.;v=_v;{ { + gk = gbar * m * m * h * modulationDA ( _threadargs_ ) ; + modShift = modulationACh ( _threadargs_ ) ; + ik = gk * ( v - ek ) ; + } + _current += ik; + +} return _current; +} + +static void nrn_cur(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; double _rhs, _v; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + ek = _ion_ek; + _g = _nrn_current(_p, _ppvar, _thread, _nt, _v + .001); + { double _dik; + _dik = ik; + _rhs = _nrn_current(_p, _ppvar, _thread, _nt, _v); + _ion_dikdv += (_dik - ik)/.001 ; + } + _g = (_g - _rhs)/.001; + _ion_ik += ik ; +#if CACHEVEC + if (use_cachevec) { + VEC_RHS(_ni[_iml]) -= _rhs; + }else +#endif + { + NODERHS(_nd) -= _rhs; + } + +} + +} + +static void nrn_jacob(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; +#if CACHEVEC + if (use_cachevec) { + VEC_D(_ni[_iml]) += _g; + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + NODED(_nd) += _g; + } + +} + +} + +static void nrn_state(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v = 0.0; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v=_v; +{ + ek = _ion_ek; + { states(_p, _ppvar, _thread, _nt); + } }} + +} + +static void terminal(){} + +static void _initlists(){ + double _x; double* _p = &_x; + int _i; static int _first = 1; + if (!_first) return; + _slist1[0] = m_columnindex; _dlist1[0] = Dm_columnindex; + _slist1[1] = h_columnindex; _dlist1[1] = Dh_columnindex; +_first = 0; +} + +#if defined(__cplusplus) +} /* extern "C" */ +#endif + +#if NMODL_TEXT +static const char* nmodl_filename = "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/kaf_ms.mod"; +static const char* nmodl_file_text = + "TITLE Fast A-type potassium current (Kv4.2)\n" + "\n" + "COMMENT\n" + "\n" + "Neuromodulation is added as functions:\n" + " \n" + " modulationDA = 1 + modDA*(maxModDA-1)*levelDA\n" + "\n" + "where:\n" + " \n" + " modDA [0]: is a switch for turning modulation on or off {1/0}\n" + " maxModDA [1]: is the maximum modulation for this specific channel (read from the param file)\n" + " e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf}\n" + " levelDA [0]: is an additional parameter for scaling modulation. \n" + " Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1}\n" + " \n" + " Further neuromodulators can be added by for example:\n" + " modulationDA = 1 + modDA*(maxModDA-1)\n" + " modulationACh = 1 + modACh*(maxModACh-1)\n" + " ....\n" + "\n" + " etc. for other neuromodulators\n" + " \n" + " \n" + " \n" + "[] == default values\n" + "{} == ranges\n" + " \n" + "ENDCOMMENT\n" + "\n" + "\n" + "NEURON {\n" + " SUFFIX kaf_ms\n" + " USEION k READ ek WRITE ik\n" + " RANGE gbar, gk, ik, q\n" + " RANGE modDA, maxModDA, levelDA\n" + " RANGE modACh, maxModACh, levelACh\n" + " RANGE modShift\n" + "}\n" + "\n" + "UNITS {\n" + " (S) = (siemens)\n" + " (mV) = (millivolt)\n" + " (mA) = (milliamp)\n" + "}\n" + "\n" + "PARAMETER {\n" + " gbar = 0.0 (S/cm2) \n" + " q = 1 : room temperature (unspecified)\n" + " :q = 2 : body temperature 35 C (Du 2017)\n" + " :q = 3 : body temperature 35 C\n" + " modDA = 0\n" + " maxModDA = 1\n" + " levelDA = 0\n" + " modShift = 0\n" + " modACh = 0\n" + " maxModACh = 1\n" + " levelACh = 0\n" + "\n" + "\n" + "\n" + "}\n" + "\n" + "ASSIGNED {\n" + " v (mV)\n" + " ek (mV)\n" + " ik (mA/cm2)\n" + " gk (S/cm2)\n" + " minf\n" + " mtau (ms)\n" + " hinf\n" + " htau (ms)\n" + "}\n" + "\n" + "STATE { m h }\n" + "\n" + "BREAKPOINT {\n" + " SOLVE states METHOD cnexp\n" + " gk = gbar*m*m*h*modulationDA()\n" + " modShift = modulationACh() \n" + " ik = gk*(v-ek)\n" + "}\n" + "\n" + "DERIVATIVE states {\n" + " rates()\n" + " m' = (minf-m)/mtau*q\n" + " h' = (hinf-h)/htau*q\n" + "}\n" + "\n" + "INITIAL {\n" + " rates()\n" + " m = minf\n" + " h = hinf\n" + "}\n" + "\n" + "PROCEDURE rates() {\n" + " UNITSOFF\n" + " minf = 1/(1+exp((v-(-10+modShift))/(-17.7)))\n" + " mtau = 0.9+1.1/(1+exp((v-(-30))/10))\n" + " hinf = 1/(1+exp((v-(-75.6))/11.8))\n" + " htau = 14\n" + " UNITSON\n" + "\n" + " :Du 2017\n" + " :LOCAL alpha, beta, sum\n" + " :UNITSOFF\n" + " :alpha = 1.5/(1+exp((v-4)/(-17)))\n" + " :beta = 0.6/(1+exp((v-10)/9))\n" + " :sum = alpha+beta\n" + " :minf = alpha/sum\n" + " :mtau = 1/sum\n" + " :\n" + " :alpha = 0.105/(1+exp((v-(-121))/22))\n" + " :beta = 0.065/(1+exp((v-(-55))/(-11)))\n" + " :sum = alpha+beta\n" + " :hinf = alpha/sum\n" + " :htau = 1/sum\n" + " :UNITSON\n" + "}\n" + "\n" + "\n" + "FUNCTION modulationDA() {\n" + " : returns modulation factor\n" + " \n" + " modulationDA = 1 + modDA*(maxModDA-1)*levelDA \n" + "}\n" + "\n" + "FUNCTION modulationACh() {\n" + " : returns modulation factor\n" + " \n" + " modulationACh = 1 + modACh*(maxModACh-1)*levelACh \n" + "}\n" + "\n" + "COMMENT\n" + "\n" + "Original data by Tkatch et al (2000) [1]. Neostriatal neurons were acutely\n" + "dissociated from young adult rats, age P28-P42. Electrophysiological\n" + "recordings were done at unspecified temperature (room temperature 20-22 C\n" + "assumed). Potentials were not corrected for the liquid junction potential\n" + "(estimated 1-2 mV).\n" + "\n" + "Activation m^1 matches experimental data [1, Fig.2C]. Activation time\n" + "constants fit tabulated data [1, Fig.2B]. Slope of inactivation function\n" + "fitted to the data [1, Fig.3B] with half inactivation potential -75.6\n" + "mV. Temperature factor q between 1.5 [3] and 3 [2] was used for body\n" + "temperature. Conductance kinetics of m2h type is used [2], no corrections\n" + "for m^2 applied. Later modification by Du [4] is close to this model.\n" + "\n" + "[1] Tkatch T, Baranauskas G, Surmeier DJ (2000) Kv4.2 mRNA abundance and\n" + "A-type K(+) current amplitude are linearly related in basal ganglia and\n" + "basal forebrain neurons. J Neurosci 20(2):579-88.\n" + "\n" + "[2] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M,\n" + "O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions\n" + "and entrainment to oscillations in a computational model of the nucleus\n" + "accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95.\n" + "\n" + "[3] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH,\n" + "Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on\n" + "calcium influx and spike timing-dependent plasticity in striatal medium\n" + "spiny neurons. PLoS Comput Biol 8(4):e1002493.\n" + "\n" + "[4] Du K, Wu YW, Lindroos R, Liu Y, R\n" + "\n" + "zsa B, Katona G, Ding JB,\n" + "Kotaleski JH (2017) Cell-type-specific inhibition of the dendritic\n" + "plateau potential in striatal spiny projection neurons. Proc Natl Acad\n" + "Sci USA 114:E7612-E7621.\n" + "\n" + "ENDCOMMENT\n" + ; +#endif diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/kaf_ms.o b/examples/example_models/str-dspn-model1/mechanisms/x86_64/kaf_ms.o new file mode 100644 index 00000000..498f162a Binary files /dev/null and b/examples/example_models/str-dspn-model1/mechanisms/x86_64/kaf_ms.o differ diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/kas_ms.c b/examples/example_models/str-dspn-model1/mechanisms/x86_64/kas_ms.c new file mode 100644 index 00000000..9f3c0446 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/x86_64/kas_ms.c @@ -0,0 +1,692 @@ +/* Created by Language version: 7.7.0 */ +/* VECTORIZED */ +#define NRN_VECTORIZED 1 +#include +#include +#include +#include "mech_api.h" +#undef PI +#define nil 0 +#include "md1redef.h" +#include "section.h" +#include "nrniv_mf.h" +#include "md2redef.h" + +#if METHOD3 +extern int _method3; +#endif + +#if !NRNGPU +#undef exp +#define exp hoc_Exp +extern double hoc_Exp(double); +#endif + +#define nrn_init _nrn_init__kas_ms +#define _nrn_initial _nrn_initial__kas_ms +#define nrn_cur _nrn_cur__kas_ms +#define _nrn_current _nrn_current__kas_ms +#define nrn_jacob _nrn_jacob__kas_ms +#define nrn_state _nrn_state__kas_ms +#define _net_receive _net_receive__kas_ms +#define rates rates__kas_ms +#define states states__kas_ms + +#define _threadargscomma_ _p, _ppvar, _thread, _nt, +#define _threadargsprotocomma_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, +#define _threadargs_ _p, _ppvar, _thread, _nt +#define _threadargsproto_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt + /*SUPPRESS 761*/ + /*SUPPRESS 762*/ + /*SUPPRESS 763*/ + /*SUPPRESS 765*/ + extern double *getarg(); + /* Thread safe. No static _p or _ppvar. */ + +#define t _nt->_t +#define dt _nt->_dt +#define gbar _p[0] +#define gbar_columnindex 0 +#define modDA _p[1] +#define modDA_columnindex 1 +#define maxModDA _p[2] +#define maxModDA_columnindex 2 +#define levelDA _p[3] +#define levelDA_columnindex 3 +#define ik _p[4] +#define ik_columnindex 4 +#define gk _p[5] +#define gk_columnindex 5 +#define m _p[6] +#define m_columnindex 6 +#define h _p[7] +#define h_columnindex 7 +#define ek _p[8] +#define ek_columnindex 8 +#define minf _p[9] +#define minf_columnindex 9 +#define mtau _p[10] +#define mtau_columnindex 10 +#define hinf _p[11] +#define hinf_columnindex 11 +#define htau _p[12] +#define htau_columnindex 12 +#define Dm _p[13] +#define Dm_columnindex 13 +#define Dh _p[14] +#define Dh_columnindex 14 +#define v _p[15] +#define v_columnindex 15 +#define _g _p[16] +#define _g_columnindex 16 +#define _ion_ek *_ppvar[0]._pval +#define _ion_ik *_ppvar[1]._pval +#define _ion_dikdv *_ppvar[2]._pval + +#if MAC +#if !defined(v) +#define v _mlhv +#endif +#if !defined(h) +#define h _mlhh +#endif +#endif + +#if defined(__cplusplus) +extern "C" { +#endif + static int hoc_nrnpointerindex = -1; + static Datum* _extcall_thread; + static Prop* _extcall_prop; + /* external NEURON variables */ + /* declaration of user functions */ + static void _hoc_modulationDA(void); + static void _hoc_rates(void); + static int _mechtype; +extern void _nrn_cacheloop_reg(int, int); +extern void hoc_register_prop_size(int, int, int); +extern void hoc_register_limits(int, HocParmLimits*); +extern void hoc_register_units(int, HocParmUnits*); +extern void nrn_promote(Prop*, int, int); +extern Memb_func* memb_func; + +#define NMODL_TEXT 1 +#if NMODL_TEXT +static const char* nmodl_file_text; +static const char* nmodl_filename; +extern void hoc_reg_nmodl_text(int, const char*); +extern void hoc_reg_nmodl_filename(int, const char*); +#endif + + extern void _nrn_setdata_reg(int, void(*)(Prop*)); + static void _setdata(Prop* _prop) { + _extcall_prop = _prop; + } + static void _hoc_setdata() { + Prop *_prop, *hoc_getdata_range(int); + _prop = hoc_getdata_range(_mechtype); + _setdata(_prop); + hoc_retpushx(1.); +} + /* connect user functions to hoc names */ + static VoidFunc hoc_intfunc[] = { + "setdata_kas_ms", _hoc_setdata, + "modulationDA_kas_ms", _hoc_modulationDA, + "rates_kas_ms", _hoc_rates, + 0, 0 +}; +#define modulationDA modulationDA_kas_ms + extern double modulationDA( _threadargsproto_ ); + /* declare global and static user variables */ +#define a a_kas_ms + double a = 0.8; +#define q q_kas_ms + double q = 3; + /* some parameters have upper and lower limits */ + static HocParmLimits _hoc_parm_limits[] = { + 0,0,0 +}; + static HocParmUnits _hoc_parm_units[] = { + "gbar_kas_ms", "S/cm2", + "ik_kas_ms", "mA/cm2", + "gk_kas_ms", "S/cm2", + 0,0 +}; + static double delta_t = 0.01; + static double h0 = 0; + static double m0 = 0; + /* connect global user variables to hoc */ + static DoubScal hoc_scdoub[] = { + "a_kas_ms", &a_kas_ms, + "q_kas_ms", &q_kas_ms, + 0,0 +}; + static DoubVec hoc_vdoub[] = { + 0,0,0 +}; + static double _sav_indep; + static void nrn_alloc(Prop*); +static void nrn_init(NrnThread*, _Memb_list*, int); +static void nrn_state(NrnThread*, _Memb_list*, int); + static void nrn_cur(NrnThread*, _Memb_list*, int); +static void nrn_jacob(NrnThread*, _Memb_list*, int); + +static int _ode_count(int); +static void _ode_map(int, double**, double**, double*, Datum*, double*, int); +static void _ode_spec(NrnThread*, _Memb_list*, int); +static void _ode_matsol(NrnThread*, _Memb_list*, int); + +#define _cvode_ieq _ppvar[3]._i + static void _ode_matsol_instance1(_threadargsproto_); + /* connect range variables in _p that hoc is supposed to know about */ + static const char *_mechanism[] = { + "7.7.0", +"kas_ms", + "gbar_kas_ms", + "modDA_kas_ms", + "maxModDA_kas_ms", + "levelDA_kas_ms", + 0, + "ik_kas_ms", + "gk_kas_ms", + 0, + "m_kas_ms", + "h_kas_ms", + 0, + 0}; + static Symbol* _k_sym; + +extern Prop* need_memb(Symbol*); + +static void nrn_alloc(Prop* _prop) { + Prop *prop_ion; + double *_p; Datum *_ppvar; + _p = nrn_prop_data_alloc(_mechtype, 17, _prop); + /*initialize range parameters*/ + gbar = 0; + modDA = 0; + maxModDA = 1; + levelDA = 0; + _prop->param = _p; + _prop->param_size = 17; + _ppvar = nrn_prop_datum_alloc(_mechtype, 4, _prop); + _prop->dparam = _ppvar; + /*connect ionic variables to this model*/ + prop_ion = need_memb(_k_sym); + nrn_promote(prop_ion, 0, 1); + _ppvar[0]._pval = &prop_ion->param[0]; /* ek */ + _ppvar[1]._pval = &prop_ion->param[3]; /* ik */ + _ppvar[2]._pval = &prop_ion->param[4]; /* _ion_dikdv */ + +} + static void _initlists(); + /* some states have an absolute tolerance */ + static Symbol** _atollist; + static HocStateTolerance _hoc_state_tol[] = { + 0,0 +}; + static void _update_ion_pointer(Datum*); + extern Symbol* hoc_lookup(const char*); +extern void _nrn_thread_reg(int, int, void(*)(Datum*)); +extern void _nrn_thread_table_reg(int, void(*)(double*, Datum*, Datum*, NrnThread*, int)); +extern void hoc_register_tolerance(int, HocStateTolerance*, Symbol***); +extern void _cvode_abstol( Symbol**, double*, int); + + void _kas_ms_reg() { + int _vectorized = 1; + _initlists(); + ion_reg("k", -10000.); + _k_sym = hoc_lookup("k_ion"); + register_mech(_mechanism, nrn_alloc,nrn_cur, nrn_jacob, nrn_state, nrn_init, hoc_nrnpointerindex, 1); + _mechtype = nrn_get_mechtype(_mechanism[1]); + _nrn_setdata_reg(_mechtype, _setdata); + _nrn_thread_reg(_mechtype, 2, _update_ion_pointer); + #if NMODL_TEXT + hoc_reg_nmodl_text(_mechtype, nmodl_file_text); + hoc_reg_nmodl_filename(_mechtype, nmodl_filename); +#endif + hoc_register_prop_size(_mechtype, 17, 4); + hoc_register_dparam_semantics(_mechtype, 0, "k_ion"); + hoc_register_dparam_semantics(_mechtype, 1, "k_ion"); + hoc_register_dparam_semantics(_mechtype, 2, "k_ion"); + hoc_register_dparam_semantics(_mechtype, 3, "cvodeieq"); + hoc_register_cvode(_mechtype, _ode_count, _ode_map, _ode_spec, _ode_matsol); + hoc_register_tolerance(_mechtype, _hoc_state_tol, &_atollist); + hoc_register_var(hoc_scdoub, hoc_vdoub, hoc_intfunc); + ivoc_help("help ?1 kas_ms /home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/kas_ms.mod\n"); + hoc_register_limits(_mechtype, _hoc_parm_limits); + hoc_register_units(_mechtype, _hoc_parm_units); + } +static int _reset; +static char *modelname = "Slowly inactivating A-type potassium current (Kv1.2)"; + +static int error; +static int _ninits = 0; +static int _match_recurse=1; +static void _modl_cleanup(){ _match_recurse=1;} +static int rates(_threadargsproto_); + +static int _ode_spec1(_threadargsproto_); +/*static int _ode_matsol1(_threadargsproto_);*/ + static int _slist1[2], _dlist1[2]; + static int states(_threadargsproto_); + +/*CVODE*/ + static int _ode_spec1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) {int _reset = 0; { + rates ( _threadargs_ ) ; + Dm = ( minf - m ) / mtau * q ; + Dh = ( hinf - h ) / htau * q ; + } + return _reset; +} + static int _ode_matsol1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + rates ( _threadargs_ ) ; + Dm = Dm / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) )) ; + Dh = Dh / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / htau )*( q ) )) ; + return 0; +} + /*END CVODE*/ + static int states (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { { + rates ( _threadargs_ ) ; + m = m + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / mtau )*( q ))))*(- ( ( ( ( minf ) ) / mtau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) ) - m) ; + h = h + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / htau )*( q ))))*(- ( ( ( ( hinf ) ) / htau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / htau )*( q ) ) - h) ; + } + return 0; +} + +static int rates ( _threadargsproto_ ) { + minf = 1.0 / ( 1.0 + exp ( ( v - ( - 27.0 ) ) / ( - 16.0 ) ) ) ; + mtau = 3.4 + 89.2 * exp ( - pow( ( ( v - ( - 34.3 ) ) / 30.1 ) , 2.0 ) ) ; + hinf = 1.0 / ( 1.0 + exp ( ( v - ( - 33.5 ) ) / 21.5 ) ) ; + htau = 548.7 * 6.0 / ( exp ( ( v - ( - 96.0 ) ) / ( - 29.01 ) ) + exp ( ( v - ( - 96.0 ) ) / 100.0 ) ) ; + return 0; } + +static void _hoc_rates(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = 1.; + rates ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +double modulationDA ( _threadargsproto_ ) { + double _lmodulationDA; + _lmodulationDA = 1.0 + modDA * ( maxModDA - 1.0 ) * levelDA ; + +return _lmodulationDA; + } + +static void _hoc_modulationDA(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = modulationDA ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +static int _ode_count(int _type){ return 2;} + +static void _ode_spec(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + ek = _ion_ek; + _ode_spec1 (_p, _ppvar, _thread, _nt); + }} + +static void _ode_map(int _ieq, double** _pv, double** _pvdot, double* _pp, Datum* _ppd, double* _atol, int _type) { + double* _p; Datum* _ppvar; + int _i; _p = _pp; _ppvar = _ppd; + _cvode_ieq = _ieq; + for (_i=0; _i < 2; ++_i) { + _pv[_i] = _pp + _slist1[_i]; _pvdot[_i] = _pp + _dlist1[_i]; + _cvode_abstol(_atollist, _atol, _i); + } + } + +static void _ode_matsol_instance1(_threadargsproto_) { + _ode_matsol1 (_p, _ppvar, _thread, _nt); + } + +static void _ode_matsol(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + ek = _ion_ek; + _ode_matsol_instance1(_threadargs_); + }} + extern void nrn_update_ion_pointer(Symbol*, Datum*, int, int); + static void _update_ion_pointer(Datum* _ppvar) { + nrn_update_ion_pointer(_k_sym, _ppvar, 0, 0); + nrn_update_ion_pointer(_k_sym, _ppvar, 1, 3); + nrn_update_ion_pointer(_k_sym, _ppvar, 2, 4); + } + +static void initmodel(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + int _i; double _save;{ + h = h0; + m = m0; + { + rates ( _threadargs_ ) ; + m = minf ; + h = hinf ; + } + +} +} + +static void nrn_init(NrnThread* _nt, _Memb_list* _ml, int _type){ +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v = _v; + ek = _ion_ek; + initmodel(_p, _ppvar, _thread, _nt); + } +} + +static double _nrn_current(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, double _v){double _current=0.;v=_v;{ { + gk = gbar * m * m * ( h * a + 1.0 - a ) * modulationDA ( _threadargs_ ) ; + ik = gk * ( v - ek ) ; + } + _current += ik; + +} return _current; +} + +static void nrn_cur(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; double _rhs, _v; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + ek = _ion_ek; + _g = _nrn_current(_p, _ppvar, _thread, _nt, _v + .001); + { double _dik; + _dik = ik; + _rhs = _nrn_current(_p, _ppvar, _thread, _nt, _v); + _ion_dikdv += (_dik - ik)/.001 ; + } + _g = (_g - _rhs)/.001; + _ion_ik += ik ; +#if CACHEVEC + if (use_cachevec) { + VEC_RHS(_ni[_iml]) -= _rhs; + }else +#endif + { + NODERHS(_nd) -= _rhs; + } + +} + +} + +static void nrn_jacob(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; +#if CACHEVEC + if (use_cachevec) { + VEC_D(_ni[_iml]) += _g; + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + NODED(_nd) += _g; + } + +} + +} + +static void nrn_state(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v = 0.0; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v=_v; +{ + ek = _ion_ek; + { states(_p, _ppvar, _thread, _nt); + } }} + +} + +static void terminal(){} + +static void _initlists(){ + double _x; double* _p = &_x; + int _i; static int _first = 1; + if (!_first) return; + _slist1[0] = m_columnindex; _dlist1[0] = Dm_columnindex; + _slist1[1] = h_columnindex; _dlist1[1] = Dh_columnindex; +_first = 0; +} + +#if defined(__cplusplus) +} /* extern "C" */ +#endif + +#if NMODL_TEXT +static const char* nmodl_filename = "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/kas_ms.mod"; +static const char* nmodl_file_text = + "TITLE Slowly inactivating A-type potassium current (Kv1.2)\n" + "\n" + "COMMENT\n" + "\n" + "Neuromodulation is added as functions:\n" + " \n" + " modulationDA = 1 + modDA*(maxModDA-1)*levelDA\n" + "\n" + "where:\n" + " \n" + " modDA [0]: is a switch for turning modulation on or off {1/0}\n" + " maxModDA [1]: is the maximum modulation for this specific channel (read from the param file)\n" + " e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf}\n" + " levelDA [0]: is an additional parameter for scaling modulation. \n" + " Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1}\n" + " \n" + " Further neuromodulators can be added by for example:\n" + " modulationDA = 1 + modDA*(maxModDA-1)\n" + " modulationACh = 1 + modACh*(maxModACh-1)\n" + " ....\n" + "\n" + " etc. for other neuromodulators\n" + " \n" + " \n" + " \n" + "[] == default values\n" + "{} == ranges\n" + " \n" + "ENDCOMMENT\n" + "\n" + "\n" + "NEURON {\n" + " SUFFIX kas_ms\n" + " USEION k READ ek WRITE ik\n" + " RANGE gbar, gk, ik\n" + " RANGE modDA, maxModDA, levelDA\n" + "\n" + "}\n" + "\n" + "UNITS {\n" + " (S) = (siemens)\n" + " (mV) = (millivolt)\n" + " (mA) = (milliamp)\n" + "}\n" + "\n" + "PARAMETER {\n" + " gbar = 0.0 (S/cm2) \n" + " a = 0.8\n" + " :q = 1 : room temperature 22-24 C\n" + " q = 3 : body temperature 33 C\n" + " modDA = 0\n" + " maxModDA = 1\n" + " levelDA = 0\n" + "\n" + "}\n" + "\n" + "ASSIGNED {\n" + " v (mV)\n" + " ek (mV)\n" + " ik (mA/cm2)\n" + " gk (S/cm2)\n" + " minf\n" + " mtau (ms)\n" + " hinf\n" + " htau (ms)\n" + "}\n" + "\n" + "STATE { m h }\n" + "\n" + "BREAKPOINT {\n" + " SOLVE states METHOD cnexp\n" + " gk = gbar*m*m*(h*a+1-a)*modulationDA()\n" + " ik = gk*(v-ek)\n" + "}\n" + "\n" + "DERIVATIVE states {\n" + " rates()\n" + " m' = (minf-m)/mtau*q\n" + " h' = (hinf-h)/htau*q\n" + "}\n" + "\n" + "INITIAL {\n" + " rates()\n" + " m = minf\n" + " h = hinf\n" + "}\n" + "\n" + "PROCEDURE rates() {\n" + " UNITSOFF\n" + " minf = 1/(1+exp((v-(-27))/(-16)))\n" + " mtau = 3.4+89.2*exp(-((v-(-34.3))/30.1)^2)\n" + " hinf = 1/(1+exp((v-(-33.5))/21.5))\n" + " htau = 548.7*6/(exp((v-(-96))/(-29.01))+exp((v-(-96))/100))\n" + " : Du 2017\n" + " :LOCAL alpha, beta, sum\n" + " :UNITSOFF\n" + " :alpha = 0.25/(1+exp((v-50)/(-20)))\n" + " :beta = 0.05/(1+exp((v-(-90))/35))\n" + " :sum = alpha+beta\n" + " :minf = alpha/sum\n" + " :mtau = 1/sum\n" + " :\n" + " :alpha = 0.0025/(1+exp((v-(-95))/16))\n" + " :beta = 0.002/(1+exp((v-50)/(-70)))\n" + " :sum = alpha+beta\n" + " :hinf = a+(alpha/sum)*(1-a)\n" + " :htau = 1/sum\n" + " UNITSON\n" + "}\n" + "\n" + "FUNCTION modulationDA() {\n" + " : returns modulation factor\n" + " \n" + " modulationDA = 1 + modDA*(maxModDA-1)*levelDA \n" + "}\n" + "\n" + "\n" + "COMMENT\n" + "\n" + "Experimental data by Shen et al (2004) [1]. Medium spiny neurons were\n" + "acutely dissociated from from young adult (P21-P28) Sprague-Dawley rat\n" + "brain. All recordings were conducted at 22-24 C. No correction for the\n" + "liquid junction potential was reported.\n" + "\n" + "Conductance kinetics of m2h type is used [1,2] with partial inactivation,\n" + "m2 (a h + (1-a)). Fraction a is set to 0.8, as in [1, Fig.6B]; other\n" + "values for a are possible [2] (see also kas.mod in companion code).\n" + "Equation for htau [1] is corrected to match the authors' data [1, Fig.6B].\n" + "Time constants were corrected to body temperature with factor q=3 [1-3].\n" + "Later modification by Du [4] is close to this model with adjusted\n" + "inactivation.\n" + "\n" + "[1] Shen W, Hernandez-Lopez S, Tkatch T, Held JE, Surmeier DJ (2004)\n" + "Kv1.2-containing K+ channels regulate subthreshold excitability of\n" + "striatal medium spiny neurons. J Neurophysiol 91(3):1337-49.\n" + "\n" + "[2] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M,\n" + "O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions\n" + "and entrainment to oscillations in a computational model of the nucleus\n" + "accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95.\n" + "\n" + "[3] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of\n" + "spike timing-dependent calcium influx during corticostriatal upstates. J\n" + "Neurophysiol 110(7):1631-45.\n" + "\n" + "[4] Du K, Wu YW, Lindroos R, Liu Y, R\n" + "\n" + "zsa B, Katona G, Ding JB,\n" + "Kotaleski JH (2017) Cell-type-specific inhibition of the dendritic\n" + "plateau potential in striatal spiny projection neurons. Proc Natl Acad\n" + "Sci USA 114:E7612-E7621.\n" + "\n" + "ENDCOMMENT\n" + ; +#endif diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/kas_ms.o b/examples/example_models/str-dspn-model1/mechanisms/x86_64/kas_ms.o new file mode 100644 index 00000000..d0c95b73 Binary files /dev/null and b/examples/example_models/str-dspn-model1/mechanisms/x86_64/kas_ms.o differ diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/kdr_ms.c b/examples/example_models/str-dspn-model1/mechanisms/x86_64/kdr_ms.c new file mode 100644 index 00000000..db851cdf --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/x86_64/kdr_ms.c @@ -0,0 +1,564 @@ +/* Created by Language version: 7.7.0 */ +/* VECTORIZED */ +#define NRN_VECTORIZED 1 +#include +#include +#include +#include "mech_api.h" +#undef PI +#define nil 0 +#include "md1redef.h" +#include "section.h" +#include "nrniv_mf.h" +#include "md2redef.h" + +#if METHOD3 +extern int _method3; +#endif + +#if !NRNGPU +#undef exp +#define exp hoc_Exp +extern double hoc_Exp(double); +#endif + +#define nrn_init _nrn_init__kdr_ms +#define _nrn_initial _nrn_initial__kdr_ms +#define nrn_cur _nrn_cur__kdr_ms +#define _nrn_current _nrn_current__kdr_ms +#define nrn_jacob _nrn_jacob__kdr_ms +#define nrn_state _nrn_state__kdr_ms +#define _net_receive _net_receive__kdr_ms +#define rates rates__kdr_ms +#define states states__kdr_ms + +#define _threadargscomma_ _p, _ppvar, _thread, _nt, +#define _threadargsprotocomma_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, +#define _threadargs_ _p, _ppvar, _thread, _nt +#define _threadargsproto_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt + /*SUPPRESS 761*/ + /*SUPPRESS 762*/ + /*SUPPRESS 763*/ + /*SUPPRESS 765*/ + extern double *getarg(); + /* Thread safe. No static _p or _ppvar. */ + +#define t _nt->_t +#define dt _nt->_dt +#define gbar _p[0] +#define gbar_columnindex 0 +#define ik _p[1] +#define ik_columnindex 1 +#define gk _p[2] +#define gk_columnindex 2 +#define m _p[3] +#define m_columnindex 3 +#define ek _p[4] +#define ek_columnindex 4 +#define minf _p[5] +#define minf_columnindex 5 +#define mtau _p[6] +#define mtau_columnindex 6 +#define Dm _p[7] +#define Dm_columnindex 7 +#define v _p[8] +#define v_columnindex 8 +#define _g _p[9] +#define _g_columnindex 9 +#define _ion_ek *_ppvar[0]._pval +#define _ion_ik *_ppvar[1]._pval +#define _ion_dikdv *_ppvar[2]._pval + +#if MAC +#if !defined(v) +#define v _mlhv +#endif +#if !defined(h) +#define h _mlhh +#endif +#endif + +#if defined(__cplusplus) +extern "C" { +#endif + static int hoc_nrnpointerindex = -1; + static Datum* _extcall_thread; + static Prop* _extcall_prop; + /* external NEURON variables */ + /* declaration of user functions */ + static void _hoc_rates(void); + static int _mechtype; +extern void _nrn_cacheloop_reg(int, int); +extern void hoc_register_prop_size(int, int, int); +extern void hoc_register_limits(int, HocParmLimits*); +extern void hoc_register_units(int, HocParmUnits*); +extern void nrn_promote(Prop*, int, int); +extern Memb_func* memb_func; + +#define NMODL_TEXT 1 +#if NMODL_TEXT +static const char* nmodl_file_text; +static const char* nmodl_filename; +extern void hoc_reg_nmodl_text(int, const char*); +extern void hoc_reg_nmodl_filename(int, const char*); +#endif + + extern void _nrn_setdata_reg(int, void(*)(Prop*)); + static void _setdata(Prop* _prop) { + _extcall_prop = _prop; + } + static void _hoc_setdata() { + Prop *_prop, *hoc_getdata_range(int); + _prop = hoc_getdata_range(_mechtype); + _setdata(_prop); + hoc_retpushx(1.); +} + /* connect user functions to hoc names */ + static VoidFunc hoc_intfunc[] = { + "setdata_kdr_ms", _hoc_setdata, + "rates_kdr_ms", _hoc_rates, + 0, 0 +}; + /* declare global and static user variables */ +#define q q_kdr_ms + double q = 1; + /* some parameters have upper and lower limits */ + static HocParmLimits _hoc_parm_limits[] = { + 0,0,0 +}; + static HocParmUnits _hoc_parm_units[] = { + "gbar_kdr_ms", "S/cm2", + "ik_kdr_ms", "mA/cm2", + "gk_kdr_ms", "S/cm2", + 0,0 +}; + static double delta_t = 0.01; + static double m0 = 0; + /* connect global user variables to hoc */ + static DoubScal hoc_scdoub[] = { + "q_kdr_ms", &q_kdr_ms, + 0,0 +}; + static DoubVec hoc_vdoub[] = { + 0,0,0 +}; + static double _sav_indep; + static void nrn_alloc(Prop*); +static void nrn_init(NrnThread*, _Memb_list*, int); +static void nrn_state(NrnThread*, _Memb_list*, int); + static void nrn_cur(NrnThread*, _Memb_list*, int); +static void nrn_jacob(NrnThread*, _Memb_list*, int); + +static int _ode_count(int); +static void _ode_map(int, double**, double**, double*, Datum*, double*, int); +static void _ode_spec(NrnThread*, _Memb_list*, int); +static void _ode_matsol(NrnThread*, _Memb_list*, int); + +#define _cvode_ieq _ppvar[3]._i + static void _ode_matsol_instance1(_threadargsproto_); + /* connect range variables in _p that hoc is supposed to know about */ + static const char *_mechanism[] = { + "7.7.0", +"kdr_ms", + "gbar_kdr_ms", + 0, + "ik_kdr_ms", + "gk_kdr_ms", + 0, + "m_kdr_ms", + 0, + 0}; + static Symbol* _k_sym; + +extern Prop* need_memb(Symbol*); + +static void nrn_alloc(Prop* _prop) { + Prop *prop_ion; + double *_p; Datum *_ppvar; + _p = nrn_prop_data_alloc(_mechtype, 10, _prop); + /*initialize range parameters*/ + gbar = 0; + _prop->param = _p; + _prop->param_size = 10; + _ppvar = nrn_prop_datum_alloc(_mechtype, 4, _prop); + _prop->dparam = _ppvar; + /*connect ionic variables to this model*/ + prop_ion = need_memb(_k_sym); + nrn_promote(prop_ion, 0, 1); + _ppvar[0]._pval = &prop_ion->param[0]; /* ek */ + _ppvar[1]._pval = &prop_ion->param[3]; /* ik */ + _ppvar[2]._pval = &prop_ion->param[4]; /* _ion_dikdv */ + +} + static void _initlists(); + /* some states have an absolute tolerance */ + static Symbol** _atollist; + static HocStateTolerance _hoc_state_tol[] = { + 0,0 +}; + static void _update_ion_pointer(Datum*); + extern Symbol* hoc_lookup(const char*); +extern void _nrn_thread_reg(int, int, void(*)(Datum*)); +extern void _nrn_thread_table_reg(int, void(*)(double*, Datum*, Datum*, NrnThread*, int)); +extern void hoc_register_tolerance(int, HocStateTolerance*, Symbol***); +extern void _cvode_abstol( Symbol**, double*, int); + + void _kdr_ms_reg() { + int _vectorized = 1; + _initlists(); + ion_reg("k", -10000.); + _k_sym = hoc_lookup("k_ion"); + register_mech(_mechanism, nrn_alloc,nrn_cur, nrn_jacob, nrn_state, nrn_init, hoc_nrnpointerindex, 1); + _mechtype = nrn_get_mechtype(_mechanism[1]); + _nrn_setdata_reg(_mechtype, _setdata); + _nrn_thread_reg(_mechtype, 2, _update_ion_pointer); + #if NMODL_TEXT + hoc_reg_nmodl_text(_mechtype, nmodl_file_text); + hoc_reg_nmodl_filename(_mechtype, nmodl_filename); +#endif + hoc_register_prop_size(_mechtype, 10, 4); + hoc_register_dparam_semantics(_mechtype, 0, "k_ion"); + hoc_register_dparam_semantics(_mechtype, 1, "k_ion"); + hoc_register_dparam_semantics(_mechtype, 2, "k_ion"); + hoc_register_dparam_semantics(_mechtype, 3, "cvodeieq"); + hoc_register_cvode(_mechtype, _ode_count, _ode_map, _ode_spec, _ode_matsol); + hoc_register_tolerance(_mechtype, _hoc_state_tol, &_atollist); + hoc_register_var(hoc_scdoub, hoc_vdoub, hoc_intfunc); + ivoc_help("help ?1 kdr_ms /home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/kdr_ms.mod\n"); + hoc_register_limits(_mechtype, _hoc_parm_limits); + hoc_register_units(_mechtype, _hoc_parm_units); + } +static int _reset; +static char *modelname = "Fast delayed rectifier potassium current (Kv3.1/3.2)"; + +static int error; +static int _ninits = 0; +static int _match_recurse=1; +static void _modl_cleanup(){ _match_recurse=1;} +static int rates(_threadargsproto_); + +static int _ode_spec1(_threadargsproto_); +/*static int _ode_matsol1(_threadargsproto_);*/ + static int _slist1[1], _dlist1[1]; + static int states(_threadargsproto_); + +/*CVODE*/ + static int _ode_spec1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) {int _reset = 0; { + rates ( _threadargs_ ) ; + Dm = ( minf - m ) / mtau * q ; + } + return _reset; +} + static int _ode_matsol1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + rates ( _threadargs_ ) ; + Dm = Dm / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) )) ; + return 0; +} + /*END CVODE*/ + static int states (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { { + rates ( _threadargs_ ) ; + m = m + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / mtau )*( q ))))*(- ( ( ( ( minf ) ) / mtau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) ) - m) ; + } + return 0; +} + +static int rates ( _threadargsproto_ ) { + minf = 1.0 / ( 1.0 + exp ( ( v - ( - 13.0 ) ) / ( - 6.0 ) ) ) ; + mtau = 11.1 ; + return 0; } + +static void _hoc_rates(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = 1.; + rates ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +static int _ode_count(int _type){ return 1;} + +static void _ode_spec(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + ek = _ion_ek; + _ode_spec1 (_p, _ppvar, _thread, _nt); + }} + +static void _ode_map(int _ieq, double** _pv, double** _pvdot, double* _pp, Datum* _ppd, double* _atol, int _type) { + double* _p; Datum* _ppvar; + int _i; _p = _pp; _ppvar = _ppd; + _cvode_ieq = _ieq; + for (_i=0; _i < 1; ++_i) { + _pv[_i] = _pp + _slist1[_i]; _pvdot[_i] = _pp + _dlist1[_i]; + _cvode_abstol(_atollist, _atol, _i); + } + } + +static void _ode_matsol_instance1(_threadargsproto_) { + _ode_matsol1 (_p, _ppvar, _thread, _nt); + } + +static void _ode_matsol(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + ek = _ion_ek; + _ode_matsol_instance1(_threadargs_); + }} + extern void nrn_update_ion_pointer(Symbol*, Datum*, int, int); + static void _update_ion_pointer(Datum* _ppvar) { + nrn_update_ion_pointer(_k_sym, _ppvar, 0, 0); + nrn_update_ion_pointer(_k_sym, _ppvar, 1, 3); + nrn_update_ion_pointer(_k_sym, _ppvar, 2, 4); + } + +static void initmodel(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + int _i; double _save;{ + m = m0; + { + rates ( _threadargs_ ) ; + m = minf ; + } + +} +} + +static void nrn_init(NrnThread* _nt, _Memb_list* _ml, int _type){ +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v = _v; + ek = _ion_ek; + initmodel(_p, _ppvar, _thread, _nt); + } +} + +static double _nrn_current(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, double _v){double _current=0.;v=_v;{ { + gk = gbar * m ; + ik = gk * ( v - ek ) ; + } + _current += ik; + +} return _current; +} + +static void nrn_cur(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; double _rhs, _v; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + ek = _ion_ek; + _g = _nrn_current(_p, _ppvar, _thread, _nt, _v + .001); + { double _dik; + _dik = ik; + _rhs = _nrn_current(_p, _ppvar, _thread, _nt, _v); + _ion_dikdv += (_dik - ik)/.001 ; + } + _g = (_g - _rhs)/.001; + _ion_ik += ik ; +#if CACHEVEC + if (use_cachevec) { + VEC_RHS(_ni[_iml]) -= _rhs; + }else +#endif + { + NODERHS(_nd) -= _rhs; + } + +} + +} + +static void nrn_jacob(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; +#if CACHEVEC + if (use_cachevec) { + VEC_D(_ni[_iml]) += _g; + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + NODED(_nd) += _g; + } + +} + +} + +static void nrn_state(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v = 0.0; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v=_v; +{ + ek = _ion_ek; + { states(_p, _ppvar, _thread, _nt); + } }} + +} + +static void terminal(){} + +static void _initlists(){ + double _x; double* _p = &_x; + int _i; static int _first = 1; + if (!_first) return; + _slist1[0] = m_columnindex; _dlist1[0] = Dm_columnindex; +_first = 0; +} + +#if defined(__cplusplus) +} /* extern "C" */ +#endif + +#if NMODL_TEXT +static const char* nmodl_filename = "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/kdr_ms.mod"; +static const char* nmodl_file_text = + "TITLE Fast delayed rectifier potassium current (Kv3.1/3.2)\n" + "\n" + "NEURON {\n" + " SUFFIX kdr_ms\n" + " USEION k READ ek WRITE ik\n" + " RANGE gbar, gk, ik\n" + "}\n" + "\n" + "UNITS {\n" + " (S) = (siemens)\n" + " (mV) = (millivolt)\n" + " (mA) = (milliamp)\n" + "}\n" + "\n" + "PARAMETER {\n" + " gbar = 0.0 (S/cm2) \n" + " q = 1\n" + "}\n" + "\n" + "ASSIGNED {\n" + " v (mV)\n" + " ek (mV)\n" + " ik (mA/cm2)\n" + " gk (S/cm2)\n" + " minf\n" + " mtau (ms)\n" + "}\n" + "\n" + "STATE { m }\n" + "\n" + "BREAKPOINT {\n" + " SOLVE states METHOD cnexp\n" + " gk = gbar*m\n" + " ik = gk*(v-ek)\n" + "}\n" + "\n" + "DERIVATIVE states {\n" + " rates()\n" + " m' = (minf-m)/mtau*q\n" + "}\n" + "\n" + "INITIAL {\n" + " rates()\n" + " m = minf\n" + "}\n" + "\n" + "PROCEDURE rates() {\n" + " UNITSOFF\n" + " minf = 1/(1+exp((v-(-13))/(-6)))\n" + " mtau = 11.1\n" + " UNITSON\n" + "}\n" + "\n" + "COMMENT\n" + "\n" + "Original data and model by Baranauskas et al (1999) [1] for globus\n" + "pallidus neurons from young adult rat. The temperature was not\n" + "specified. Potentials were not corrected for the liquid junction\n" + "potential, which was estimated to be 1-2 mV.\n" + "\n" + "Kinetics of m^1 type is used as in [2,3]. Room temperature 20-23 C\n" + "is assumed.\n" + "\n" + "NEURON implementation by Alexander Kozlov .\n" + "\n" + "[1] Baranauskas G, Tkatch T, Surmeier DJ (1999) Delayed rectifier currents\n" + "in rat globus pallidus neurons are attributable to Kv2.1 and Kv3.1/3.2\n" + "K(+) channels. J Neurosci 19(15):6394-404.\n" + "\n" + "[2] Migliore M, Hoffman DA, Magee JC, Johnston D (1999) Role of an\n" + "A-type K+ conductance in the back-propagation of action potentials in the\n" + "dendrites of hippocampal pyramidal neurons. J Comput Neurosci 7(1):5-15.\n" + "\n" + "[3] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH,\n" + "Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on\n" + "calcium influx and spike timing-dependent plasticity in striatal medium\n" + "spiny neurons. PLoS Comput Biol 8(4):e1002493.\n" + "\n" + "ENDCOMMENT\n" + ; +#endif diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/kdr_ms.o b/examples/example_models/str-dspn-model1/mechanisms/x86_64/kdr_ms.o new file mode 100644 index 00000000..e41d4f13 Binary files /dev/null and b/examples/example_models/str-dspn-model1/mechanisms/x86_64/kdr_ms.o differ diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/kir_ms.c b/examples/example_models/str-dspn-model1/mechanisms/x86_64/kir_ms.c new file mode 100644 index 00000000..e725a78a --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/x86_64/kir_ms.c @@ -0,0 +1,703 @@ +/* Created by Language version: 7.7.0 */ +/* VECTORIZED */ +#define NRN_VECTORIZED 1 +#include +#include +#include +#include "mech_api.h" +#undef PI +#define nil 0 +#include "md1redef.h" +#include "section.h" +#include "nrniv_mf.h" +#include "md2redef.h" + +#if METHOD3 +extern int _method3; +#endif + +#if !NRNGPU +#undef exp +#define exp hoc_Exp +extern double hoc_Exp(double); +#endif + +#define nrn_init _nrn_init__kir_ms +#define _nrn_initial _nrn_initial__kir_ms +#define nrn_cur _nrn_cur__kir_ms +#define _nrn_current _nrn_current__kir_ms +#define nrn_jacob _nrn_jacob__kir_ms +#define nrn_state _nrn_state__kir_ms +#define _net_receive _net_receive__kir_ms +#define rates rates__kir_ms +#define states states__kir_ms + +#define _threadargscomma_ _p, _ppvar, _thread, _nt, +#define _threadargsprotocomma_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, +#define _threadargs_ _p, _ppvar, _thread, _nt +#define _threadargsproto_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt + /*SUPPRESS 761*/ + /*SUPPRESS 762*/ + /*SUPPRESS 763*/ + /*SUPPRESS 765*/ + extern double *getarg(); + /* Thread safe. No static _p or _ppvar. */ + +#define t _nt->_t +#define dt _nt->_dt +#define gbar _p[0] +#define gbar_columnindex 0 +#define shift _p[1] +#define shift_columnindex 1 +#define modDA _p[2] +#define modDA_columnindex 2 +#define maxModDA _p[3] +#define maxModDA_columnindex 3 +#define levelDA _p[4] +#define levelDA_columnindex 4 +#define modACh _p[5] +#define modACh_columnindex 5 +#define maxModACh _p[6] +#define maxModACh_columnindex 6 +#define levelACh _p[7] +#define levelACh_columnindex 7 +#define ik _p[8] +#define ik_columnindex 8 +#define gk _p[9] +#define gk_columnindex 9 +#define m _p[10] +#define m_columnindex 10 +#define ek _p[11] +#define ek_columnindex 11 +#define minf _p[12] +#define minf_columnindex 12 +#define mtau _p[13] +#define mtau_columnindex 13 +#define Dm _p[14] +#define Dm_columnindex 14 +#define v _p[15] +#define v_columnindex 15 +#define _g _p[16] +#define _g_columnindex 16 +#define _ion_ek *_ppvar[0]._pval +#define _ion_ik *_ppvar[1]._pval +#define _ion_dikdv *_ppvar[2]._pval + +#if MAC +#if !defined(v) +#define v _mlhv +#endif +#if !defined(h) +#define h _mlhh +#endif +#endif + +#if defined(__cplusplus) +extern "C" { +#endif + static int hoc_nrnpointerindex = -1; + static Datum* _extcall_thread; + static Prop* _extcall_prop; + /* external NEURON variables */ + /* declaration of user functions */ + static void _hoc_modulationACh(void); + static void _hoc_modulationDA(void); + static void _hoc_rates(void); + static int _mechtype; +extern void _nrn_cacheloop_reg(int, int); +extern void hoc_register_prop_size(int, int, int); +extern void hoc_register_limits(int, HocParmLimits*); +extern void hoc_register_units(int, HocParmUnits*); +extern void nrn_promote(Prop*, int, int); +extern Memb_func* memb_func; + +#define NMODL_TEXT 1 +#if NMODL_TEXT +static const char* nmodl_file_text; +static const char* nmodl_filename; +extern void hoc_reg_nmodl_text(int, const char*); +extern void hoc_reg_nmodl_filename(int, const char*); +#endif + + extern void _nrn_setdata_reg(int, void(*)(Prop*)); + static void _setdata(Prop* _prop) { + _extcall_prop = _prop; + } + static void _hoc_setdata() { + Prop *_prop, *hoc_getdata_range(int); + _prop = hoc_getdata_range(_mechtype); + _setdata(_prop); + hoc_retpushx(1.); +} + /* connect user functions to hoc names */ + static VoidFunc hoc_intfunc[] = { + "setdata_kir_ms", _hoc_setdata, + "modulationACh_kir_ms", _hoc_modulationACh, + "modulationDA_kir_ms", _hoc_modulationDA, + "rates_kir_ms", _hoc_rates, + 0, 0 +}; +#define modulationACh modulationACh_kir_ms +#define modulationDA modulationDA_kir_ms + extern double modulationACh( _threadargsproto_ ); + extern double modulationDA( _threadargsproto_ ); + /* declare global and static user variables */ +#define q q_kir_ms + double q = 1; + /* some parameters have upper and lower limits */ + static HocParmLimits _hoc_parm_limits[] = { + 0,0,0 +}; + static HocParmUnits _hoc_parm_units[] = { + "gbar_kir_ms", "S/cm2", + "shift_kir_ms", "mV", + "ik_kir_ms", "mA/cm2", + "gk_kir_ms", "S/cm2", + 0,0 +}; + static double delta_t = 0.01; + static double m0 = 0; + /* connect global user variables to hoc */ + static DoubScal hoc_scdoub[] = { + "q_kir_ms", &q_kir_ms, + 0,0 +}; + static DoubVec hoc_vdoub[] = { + 0,0,0 +}; + static double _sav_indep; + static void nrn_alloc(Prop*); +static void nrn_init(NrnThread*, _Memb_list*, int); +static void nrn_state(NrnThread*, _Memb_list*, int); + static void nrn_cur(NrnThread*, _Memb_list*, int); +static void nrn_jacob(NrnThread*, _Memb_list*, int); + +static int _ode_count(int); +static void _ode_map(int, double**, double**, double*, Datum*, double*, int); +static void _ode_spec(NrnThread*, _Memb_list*, int); +static void _ode_matsol(NrnThread*, _Memb_list*, int); + +#define _cvode_ieq _ppvar[3]._i + static void _ode_matsol_instance1(_threadargsproto_); + /* connect range variables in _p that hoc is supposed to know about */ + static const char *_mechanism[] = { + "7.7.0", +"kir_ms", + "gbar_kir_ms", + "shift_kir_ms", + "modDA_kir_ms", + "maxModDA_kir_ms", + "levelDA_kir_ms", + "modACh_kir_ms", + "maxModACh_kir_ms", + "levelACh_kir_ms", + 0, + "ik_kir_ms", + "gk_kir_ms", + 0, + "m_kir_ms", + 0, + 0}; + static Symbol* _k_sym; + +extern Prop* need_memb(Symbol*); + +static void nrn_alloc(Prop* _prop) { + Prop *prop_ion; + double *_p; Datum *_ppvar; + _p = nrn_prop_data_alloc(_mechtype, 17, _prop); + /*initialize range parameters*/ + gbar = 0; + shift = 0; + modDA = 0; + maxModDA = 1; + levelDA = 0; + modACh = 0; + maxModACh = 1; + levelACh = 0; + _prop->param = _p; + _prop->param_size = 17; + _ppvar = nrn_prop_datum_alloc(_mechtype, 4, _prop); + _prop->dparam = _ppvar; + /*connect ionic variables to this model*/ + prop_ion = need_memb(_k_sym); + nrn_promote(prop_ion, 0, 1); + _ppvar[0]._pval = &prop_ion->param[0]; /* ek */ + _ppvar[1]._pval = &prop_ion->param[3]; /* ik */ + _ppvar[2]._pval = &prop_ion->param[4]; /* _ion_dikdv */ + +} + static void _initlists(); + /* some states have an absolute tolerance */ + static Symbol** _atollist; + static HocStateTolerance _hoc_state_tol[] = { + 0,0 +}; + static void _update_ion_pointer(Datum*); + extern Symbol* hoc_lookup(const char*); +extern void _nrn_thread_reg(int, int, void(*)(Datum*)); +extern void _nrn_thread_table_reg(int, void(*)(double*, Datum*, Datum*, NrnThread*, int)); +extern void hoc_register_tolerance(int, HocStateTolerance*, Symbol***); +extern void _cvode_abstol( Symbol**, double*, int); + + void _kir_ms_reg() { + int _vectorized = 1; + _initlists(); + ion_reg("k", -10000.); + _k_sym = hoc_lookup("k_ion"); + register_mech(_mechanism, nrn_alloc,nrn_cur, nrn_jacob, nrn_state, nrn_init, hoc_nrnpointerindex, 1); + _mechtype = nrn_get_mechtype(_mechanism[1]); + _nrn_setdata_reg(_mechtype, _setdata); + _nrn_thread_reg(_mechtype, 2, _update_ion_pointer); + #if NMODL_TEXT + hoc_reg_nmodl_text(_mechtype, nmodl_file_text); + hoc_reg_nmodl_filename(_mechtype, nmodl_filename); +#endif + hoc_register_prop_size(_mechtype, 17, 4); + hoc_register_dparam_semantics(_mechtype, 0, "k_ion"); + hoc_register_dparam_semantics(_mechtype, 1, "k_ion"); + hoc_register_dparam_semantics(_mechtype, 2, "k_ion"); + hoc_register_dparam_semantics(_mechtype, 3, "cvodeieq"); + hoc_register_cvode(_mechtype, _ode_count, _ode_map, _ode_spec, _ode_matsol); + hoc_register_tolerance(_mechtype, _hoc_state_tol, &_atollist); + hoc_register_var(hoc_scdoub, hoc_vdoub, hoc_intfunc); + ivoc_help("help ?1 kir_ms /home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/kir_ms.mod\n"); + hoc_register_limits(_mechtype, _hoc_parm_limits); + hoc_register_units(_mechtype, _hoc_parm_units); + } +static int _reset; +static char *modelname = "Non-inactivating inwardly rectifying potassium current (Kir2.3)"; + +static int error; +static int _ninits = 0; +static int _match_recurse=1; +static void _modl_cleanup(){ _match_recurse=1;} +static int rates(_threadargsproto_); + +static int _ode_spec1(_threadargsproto_); +/*static int _ode_matsol1(_threadargsproto_);*/ + static int _slist1[1], _dlist1[1]; + static int states(_threadargsproto_); + +/*CVODE*/ + static int _ode_spec1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) {int _reset = 0; { + rates ( _threadargs_ ) ; + Dm = ( minf - m ) / mtau * q ; + } + return _reset; +} + static int _ode_matsol1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + rates ( _threadargs_ ) ; + Dm = Dm / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) )) ; + return 0; +} + /*END CVODE*/ + static int states (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { { + rates ( _threadargs_ ) ; + m = m + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / mtau )*( q ))))*(- ( ( ( ( minf ) ) / mtau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) ) - m) ; + } + return 0; +} + +static int rates ( _threadargsproto_ ) { + minf = 1.0 / ( 1.0 + exp ( ( v - ( - 82.0 ) - shift ) / 13.0 ) ) ; + mtau = 1.0 / ( exp ( ( v - ( - 103.0 ) ) / ( - 14.5 ) ) + 0.125 / ( 1.0 + exp ( ( v - ( - 35.0 ) ) / ( - 19.0 ) ) ) ) ; + return 0; } + +static void _hoc_rates(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = 1.; + rates ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +double modulationDA ( _threadargsproto_ ) { + double _lmodulationDA; + _lmodulationDA = 1.0 + modDA * ( maxModDA - 1.0 ) * levelDA ; + +return _lmodulationDA; + } + +static void _hoc_modulationDA(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = modulationDA ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +double modulationACh ( _threadargsproto_ ) { + double _lmodulationACh; + _lmodulationACh = 1.0 + modACh * ( maxModACh - 1.0 ) * levelACh ; + +return _lmodulationACh; + } + +static void _hoc_modulationACh(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = modulationACh ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +static int _ode_count(int _type){ return 1;} + +static void _ode_spec(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + ek = _ion_ek; + _ode_spec1 (_p, _ppvar, _thread, _nt); + }} + +static void _ode_map(int _ieq, double** _pv, double** _pvdot, double* _pp, Datum* _ppd, double* _atol, int _type) { + double* _p; Datum* _ppvar; + int _i; _p = _pp; _ppvar = _ppd; + _cvode_ieq = _ieq; + for (_i=0; _i < 1; ++_i) { + _pv[_i] = _pp + _slist1[_i]; _pvdot[_i] = _pp + _dlist1[_i]; + _cvode_abstol(_atollist, _atol, _i); + } + } + +static void _ode_matsol_instance1(_threadargsproto_) { + _ode_matsol1 (_p, _ppvar, _thread, _nt); + } + +static void _ode_matsol(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + ek = _ion_ek; + _ode_matsol_instance1(_threadargs_); + }} + extern void nrn_update_ion_pointer(Symbol*, Datum*, int, int); + static void _update_ion_pointer(Datum* _ppvar) { + nrn_update_ion_pointer(_k_sym, _ppvar, 0, 0); + nrn_update_ion_pointer(_k_sym, _ppvar, 1, 3); + nrn_update_ion_pointer(_k_sym, _ppvar, 2, 4); + } + +static void initmodel(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + int _i; double _save;{ + m = m0; + { + rates ( _threadargs_ ) ; + m = minf ; + } + +} +} + +static void nrn_init(NrnThread* _nt, _Memb_list* _ml, int _type){ +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v = _v; + ek = _ion_ek; + initmodel(_p, _ppvar, _thread, _nt); + } +} + +static double _nrn_current(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, double _v){double _current=0.;v=_v;{ { + gk = gbar * m * modulationDA ( _threadargs_ ) * modulationACh ( _threadargs_ ) ; + ik = gk * ( v - ek ) ; + } + _current += ik; + +} return _current; +} + +static void nrn_cur(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; double _rhs, _v; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + ek = _ion_ek; + _g = _nrn_current(_p, _ppvar, _thread, _nt, _v + .001); + { double _dik; + _dik = ik; + _rhs = _nrn_current(_p, _ppvar, _thread, _nt, _v); + _ion_dikdv += (_dik - ik)/.001 ; + } + _g = (_g - _rhs)/.001; + _ion_ik += ik ; +#if CACHEVEC + if (use_cachevec) { + VEC_RHS(_ni[_iml]) -= _rhs; + }else +#endif + { + NODERHS(_nd) -= _rhs; + } + +} + +} + +static void nrn_jacob(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; +#if CACHEVEC + if (use_cachevec) { + VEC_D(_ni[_iml]) += _g; + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + NODED(_nd) += _g; + } + +} + +} + +static void nrn_state(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v = 0.0; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v=_v; +{ + ek = _ion_ek; + { states(_p, _ppvar, _thread, _nt); + } }} + +} + +static void terminal(){} + +static void _initlists(){ + double _x; double* _p = &_x; + int _i; static int _first = 1; + if (!_first) return; + _slist1[0] = m_columnindex; _dlist1[0] = Dm_columnindex; +_first = 0; +} + +#if defined(__cplusplus) +} /* extern "C" */ +#endif + +#if NMODL_TEXT +static const char* nmodl_filename = "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/kir_ms.mod"; +static const char* nmodl_file_text = + "TITLE Non-inactivating inwardly rectifying potassium current (Kir2.3)\n" + "\n" + "COMMENT\n" + "\n" + "Neuromodulation is added as functions:\n" + " \n" + " modulationDA = 1 + modDA*(maxModDA-1)*levelDA\n" + "\n" + "where:\n" + " \n" + " modDA [0]: is a switch for turning modulation on or off {1/0}\n" + " maxModDA [1]: is the maximum modulation for this specific channel (read from the param file)\n" + " e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf}\n" + " levelDA [0]: is an additional parameter for scaling modulation. \n" + " Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1}\n" + " \n" + " Further neuromodulators can be added by for example:\n" + " modulationDA = 1 + modDA*(maxModDA-1)\n" + " modulationACh = 1 + modACh*(maxModACh-1)\n" + " ....\n" + "\n" + " etc. for other neuromodulators\n" + " \n" + " \n" + " \n" + "[] == default values\n" + "{} == ranges\n" + " \n" + "ENDCOMMENT\n" + "\n" + "\n" + "NEURON {\n" + " SUFFIX kir_ms\n" + " USEION k READ ek WRITE ik\n" + " RANGE gbar, gk, ik, shift\n" + " RANGE modDA, maxModDA, levelDA\n" + " RANGE modACh, maxModACh, levelACh\n" + "}\n" + "\n" + "UNITS {\n" + " (S) = (siemens)\n" + " (mV) = (millivolt)\n" + " (mA) = (milliamp)\n" + "}\n" + "\n" + "PARAMETER {\n" + " gbar = 0.0 (S/cm2) \n" + " shift = 0.0 (mV)\n" + " q = 1 : body temperature 35 C\n" + " modDA = 0\n" + " maxModDA = 1\n" + " levelDA = 0\n" + " modACh = 0\n" + " maxModACh = 1\n" + " levelACh = 0\n" + "}\n" + "\n" + "ASSIGNED {\n" + " v (mV)\n" + " ek (mV)\n" + " ik (mA/cm2)\n" + " gk (S/cm2)\n" + " minf\n" + " mtau (ms)\n" + "}\n" + "\n" + "STATE { m }\n" + "\n" + "BREAKPOINT {\n" + " SOLVE states METHOD cnexp\n" + " gk = gbar*m*modulationDA()*modulationACh()\n" + " ik = gk*(v-ek)\n" + "}\n" + "\n" + "DERIVATIVE states {\n" + " rates()\n" + " m' = (minf-m)/mtau*q\n" + "}\n" + "\n" + "INITIAL {\n" + " rates()\n" + " m = minf\n" + "}\n" + "\n" + "PROCEDURE rates() {\n" + " UNITSOFF\n" + " minf = 1/(1+exp((v-(-82)-shift)/13))\n" + " mtau = 1/(exp((v-(-103))/(-14.5))+0.125/(1+exp((v-(-35))/(-19))))\n" + " UNITSON\n" + "}\n" + "\n" + "FUNCTION modulationDA() {\n" + " : returns modulation factor\n" + " \n" + " modulationDA = 1 + modDA*(maxModDA-1)*levelDA \n" + "}\n" + "\n" + "FUNCTION modulationACh() {\n" + " : returns modulation factor\n" + " \n" + " modulationACh = 1 + modACh*(maxModACh-1)*levelACh \n" + "}\n" + "\n" + "COMMENT\n" + "\n" + "Original model by Wolf et al (2005) [1] for the rat MSN cells from the\n" + "nucleus accumbens. The activation curve was fitted to a mouse Kir2.1\n" + "channel expressed in HEK cells [2] and shifted to match extracellular\n" + "concentration of K in rat. Measured half-activation values are -109.3\n" + "mV (striatonigral MSN) and -113.2 mV (striatopallidal MSN) [6, Supp\n" + "Tab.1]. Time constants were derived from Aplysia data [3] and adjusted\n" + "to match the rat experiments [1]. Time constant was further tuned [4]\n" + "to fit the rat data below -80 mV [5]. Kinetics is corrected to the body\n" + "temperature 35 C [4].\n" + "\n" + "Non-inactivating Kir current was observed in cells expressing Kir2.2\n" + "and/or Kir2.3 [5]. Activation variable with m^1 kinetics is used [1,4].\n" + "Smooth fit of the time constants by Alexander Kozlov .\n" + "\n" + "[1] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M,\n" + "O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions\n" + "and entrainment to oscillations in a computational model of the nucleus\n" + "accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95.\n" + "\n" + "[2] Kubo Y, Murata Y (2001) Control of rectification and permeation by\n" + "two distinct sites after the second transmembrane region in Kir2.1 K+\n" + "channel. J Physiol 531, 645-660.\n" + "\n" + "[3] Hayashi H, Fishman HM (1988) Inward rectifier K+ channel kinetics\n" + "from analysis of the complex conductance of aplysia neuronal membrane.\n" + "Biophys J 53, 747-757.\n" + "\n" + "[4] Steephen JE, Manchanda R (2009) Differences in biophysical properties\n" + "of nucleus accumbens medium spiny neurons emerging from inactivation of\n" + "inward rectifying potassium currents. J Comput Neurosci 27(3):453-70\n" + "\n" + "[5] Mermelstein PG, Song WJ, Tkatch T, Yan Z, Surmeier DJ (1998)\n" + "Inwardly rectifying potassium (IRK) currents are correlated with IRK\n" + "subunit expression in rat nucleus accumbens medium spiny neurons. J\n" + "Neurosci 18(17):6650-61.\n" + "\n" + "[6] Shen W, Tian X, Day M, Ulrich S, Tkatch T, Nathanson NM, Surmeier DJ\n" + "(2007) Cholinergic modulation of Kir2 channels selectively elevates\n" + "dendritic excitability in striatopallidal neurons. Nat Neurosci\n" + "10(11):1458-66.\n" + "\n" + "ENDCOMMENT\n" + ; +#endif diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/kir_ms.o b/examples/example_models/str-dspn-model1/mechanisms/x86_64/kir_ms.o new file mode 100644 index 00000000..24531841 Binary files /dev/null and b/examples/example_models/str-dspn-model1/mechanisms/x86_64/kir_ms.o differ diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/makemod2c_inc b/examples/example_models/str-dspn-model1/mechanisms/x86_64/makemod2c_inc new file mode 100644 index 00000000..361c3dd4 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/x86_64/makemod2c_inc @@ -0,0 +1,136 @@ +./bk_ms.c: ../bk_ms.mod + @printf " -> $(C_GREEN)NMODL$(C_RESET) $<\\n" + (cd ".."; MODLUNIT=$(NRNUNITS) $(NOCMODL) "bk_ms.mod" -o "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/x86_64") + +./bk_ms.o: ./bk_ms.c + @printf " -> $(C_GREEN)Compiling$(C_RESET) $<\\n" + $(COMPILE) -I".." $(INCLUDES) -fPIC -c $< -o $@ + +./cadyn_ms.c: ../cadyn_ms.mod + @printf " -> $(C_GREEN)NMODL$(C_RESET) $<\\n" + (cd ".."; MODLUNIT=$(NRNUNITS) $(NOCMODL) "cadyn_ms.mod" -o "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/x86_64") + +./cadyn_ms.o: ./cadyn_ms.c + @printf " -> $(C_GREEN)Compiling$(C_RESET) $<\\n" + $(COMPILE) -I".." $(INCLUDES) -fPIC -c $< -o $@ + +./cal12_ms.c: ../cal12_ms.mod + @printf " -> $(C_GREEN)NMODL$(C_RESET) $<\\n" + (cd ".."; MODLUNIT=$(NRNUNITS) $(NOCMODL) "cal12_ms.mod" -o "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/x86_64") + +./cal12_ms.o: ./cal12_ms.c + @printf " -> $(C_GREEN)Compiling$(C_RESET) $<\\n" + $(COMPILE) -I".." $(INCLUDES) -fPIC -c $< -o $@ + +./cal13_ms.c: ../cal13_ms.mod + @printf " -> $(C_GREEN)NMODL$(C_RESET) $<\\n" + (cd ".."; MODLUNIT=$(NRNUNITS) $(NOCMODL) "cal13_ms.mod" -o "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/x86_64") + +./cal13_ms.o: ./cal13_ms.c + @printf " -> $(C_GREEN)Compiling$(C_RESET) $<\\n" + $(COMPILE) -I".." $(INCLUDES) -fPIC -c $< -o $@ + +./caldyn_ms.c: ../caldyn_ms.mod + @printf " -> $(C_GREEN)NMODL$(C_RESET) $<\\n" + (cd ".."; MODLUNIT=$(NRNUNITS) $(NOCMODL) "caldyn_ms.mod" -o "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/x86_64") + +./caldyn_ms.o: ./caldyn_ms.c + @printf " -> $(C_GREEN)Compiling$(C_RESET) $<\\n" + $(COMPILE) -I".." $(INCLUDES) -fPIC -c $< -o $@ + +./can_ms.c: ../can_ms.mod + @printf " -> $(C_GREEN)NMODL$(C_RESET) $<\\n" + (cd ".."; MODLUNIT=$(NRNUNITS) $(NOCMODL) "can_ms.mod" -o "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/x86_64") + +./can_ms.o: ./can_ms.c + @printf " -> $(C_GREEN)Compiling$(C_RESET) $<\\n" + $(COMPILE) -I".." $(INCLUDES) -fPIC -c $< -o $@ + +./caq_ms.c: ../caq_ms.mod + @printf " -> $(C_GREEN)NMODL$(C_RESET) $<\\n" + (cd ".."; MODLUNIT=$(NRNUNITS) $(NOCMODL) "caq_ms.mod" -o "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/x86_64") + +./caq_ms.o: ./caq_ms.c + @printf " -> $(C_GREEN)Compiling$(C_RESET) $<\\n" + $(COMPILE) -I".." $(INCLUDES) -fPIC -c $< -o $@ + +./car_ms.c: ../car_ms.mod + @printf " -> $(C_GREEN)NMODL$(C_RESET) $<\\n" + (cd ".."; MODLUNIT=$(NRNUNITS) $(NOCMODL) "car_ms.mod" -o "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/x86_64") + +./car_ms.o: ./car_ms.c + @printf " -> $(C_GREEN)Compiling$(C_RESET) $<\\n" + $(COMPILE) -I".." $(INCLUDES) -fPIC -c $< -o $@ + +./cat32_ms.c: ../cat32_ms.mod + @printf " -> $(C_GREEN)NMODL$(C_RESET) $<\\n" + (cd ".."; MODLUNIT=$(NRNUNITS) $(NOCMODL) "cat32_ms.mod" -o "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/x86_64") + +./cat32_ms.o: ./cat32_ms.c + @printf " -> $(C_GREEN)Compiling$(C_RESET) $<\\n" + $(COMPILE) -I".." $(INCLUDES) -fPIC -c $< -o $@ + +./cat33_ms.c: ../cat33_ms.mod + @printf " -> $(C_GREEN)NMODL$(C_RESET) $<\\n" + (cd ".."; MODLUNIT=$(NRNUNITS) $(NOCMODL) "cat33_ms.mod" -o "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/x86_64") + +./cat33_ms.o: ./cat33_ms.c + @printf " -> $(C_GREEN)Compiling$(C_RESET) $<\\n" + $(COMPILE) -I".." $(INCLUDES) -fPIC -c $< -o $@ + +./Im_ms.c: ../Im_ms.mod + @printf " -> $(C_GREEN)NMODL$(C_RESET) $<\\n" + (cd ".."; MODLUNIT=$(NRNUNITS) $(NOCMODL) "Im_ms.mod" -o "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/x86_64") + +./Im_ms.o: ./Im_ms.c + @printf " -> $(C_GREEN)Compiling$(C_RESET) $<\\n" + $(COMPILE) -I".." $(INCLUDES) -fPIC -c $< -o $@ + +./kaf_ms.c: ../kaf_ms.mod + @printf " -> $(C_GREEN)NMODL$(C_RESET) $<\\n" + (cd ".."; MODLUNIT=$(NRNUNITS) $(NOCMODL) "kaf_ms.mod" -o "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/x86_64") + +./kaf_ms.o: ./kaf_ms.c + @printf " -> $(C_GREEN)Compiling$(C_RESET) $<\\n" + $(COMPILE) -I".." $(INCLUDES) -fPIC -c $< -o $@ + +./kas_ms.c: ../kas_ms.mod + @printf " -> $(C_GREEN)NMODL$(C_RESET) $<\\n" + (cd ".."; MODLUNIT=$(NRNUNITS) $(NOCMODL) "kas_ms.mod" -o "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/x86_64") + +./kas_ms.o: ./kas_ms.c + @printf " -> $(C_GREEN)Compiling$(C_RESET) $<\\n" + $(COMPILE) -I".." $(INCLUDES) -fPIC -c $< -o $@ + +./kdr_ms.c: ../kdr_ms.mod + @printf " -> $(C_GREEN)NMODL$(C_RESET) $<\\n" + (cd ".."; MODLUNIT=$(NRNUNITS) $(NOCMODL) "kdr_ms.mod" -o "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/x86_64") + +./kdr_ms.o: ./kdr_ms.c + @printf " -> $(C_GREEN)Compiling$(C_RESET) $<\\n" + $(COMPILE) -I".." $(INCLUDES) -fPIC -c $< -o $@ + +./kir_ms.c: ../kir_ms.mod + @printf " -> $(C_GREEN)NMODL$(C_RESET) $<\\n" + (cd ".."; MODLUNIT=$(NRNUNITS) $(NOCMODL) "kir_ms.mod" -o "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/x86_64") + +./kir_ms.o: ./kir_ms.c + @printf " -> $(C_GREEN)Compiling$(C_RESET) $<\\n" + $(COMPILE) -I".." $(INCLUDES) -fPIC -c $< -o $@ + +./naf_ms.c: ../naf_ms.mod + @printf " -> $(C_GREEN)NMODL$(C_RESET) $<\\n" + (cd ".."; MODLUNIT=$(NRNUNITS) $(NOCMODL) "naf_ms.mod" -o "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/x86_64") + +./naf_ms.o: ./naf_ms.c + @printf " -> $(C_GREEN)Compiling$(C_RESET) $<\\n" + $(COMPILE) -I".." $(INCLUDES) -fPIC -c $< -o $@ + +./sk_ms.c: ../sk_ms.mod + @printf " -> $(C_GREEN)NMODL$(C_RESET) $<\\n" + (cd ".."; MODLUNIT=$(NRNUNITS) $(NOCMODL) "sk_ms.mod" -o "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/x86_64") + +./sk_ms.o: ./sk_ms.c + @printf " -> $(C_GREEN)Compiling$(C_RESET) $<\\n" + $(COMPILE) -I".." $(INCLUDES) -fPIC -c $< -o $@ + diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/mod_func.cpp b/examples/example_models/str-dspn-model1/mechanisms/x86_64/mod_func.cpp new file mode 100644 index 00000000..b88b8b1c --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/x86_64/mod_func.cpp @@ -0,0 +1,70 @@ +#include +#include "hocdec.h" +extern int nrnmpi_myid; +extern int nrn_nobanner_; +#if defined(__cplusplus) +extern "C" { +#endif + +extern void _bk_ms_reg(void); +extern void _cadyn_ms_reg(void); +extern void _cal12_ms_reg(void); +extern void _cal13_ms_reg(void); +extern void _caldyn_ms_reg(void); +extern void _can_ms_reg(void); +extern void _caq_ms_reg(void); +extern void _car_ms_reg(void); +extern void _cat32_ms_reg(void); +extern void _cat33_ms_reg(void); +extern void _Im_ms_reg(void); +extern void _kaf_ms_reg(void); +extern void _kas_ms_reg(void); +extern void _kdr_ms_reg(void); +extern void _kir_ms_reg(void); +extern void _naf_ms_reg(void); +extern void _sk_ms_reg(void); + +void modl_reg() { + if (!nrn_nobanner_) if (nrnmpi_myid < 1) { + fprintf(stderr, "Additional mechanisms from files\n"); + fprintf(stderr, " \"bk_ms.mod\""); + fprintf(stderr, " \"cadyn_ms.mod\""); + fprintf(stderr, " \"cal12_ms.mod\""); + fprintf(stderr, " \"cal13_ms.mod\""); + fprintf(stderr, " \"caldyn_ms.mod\""); + fprintf(stderr, " \"can_ms.mod\""); + fprintf(stderr, " \"caq_ms.mod\""); + fprintf(stderr, " \"car_ms.mod\""); + fprintf(stderr, " \"cat32_ms.mod\""); + fprintf(stderr, " \"cat33_ms.mod\""); + fprintf(stderr, " \"Im_ms.mod\""); + fprintf(stderr, " \"kaf_ms.mod\""); + fprintf(stderr, " \"kas_ms.mod\""); + fprintf(stderr, " \"kdr_ms.mod\""); + fprintf(stderr, " \"kir_ms.mod\""); + fprintf(stderr, " \"naf_ms.mod\""); + fprintf(stderr, " \"sk_ms.mod\""); + fprintf(stderr, "\n"); + } + _bk_ms_reg(); + _cadyn_ms_reg(); + _cal12_ms_reg(); + _cal13_ms_reg(); + _caldyn_ms_reg(); + _can_ms_reg(); + _caq_ms_reg(); + _car_ms_reg(); + _cat32_ms_reg(); + _cat33_ms_reg(); + _Im_ms_reg(); + _kaf_ms_reg(); + _kas_ms_reg(); + _kdr_ms_reg(); + _kir_ms_reg(); + _naf_ms_reg(); + _sk_ms_reg(); +} + +#if defined(__cplusplus) +} +#endif diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/mod_func.o b/examples/example_models/str-dspn-model1/mechanisms/x86_64/mod_func.o new file mode 100644 index 00000000..860a2c98 Binary files /dev/null and b/examples/example_models/str-dspn-model1/mechanisms/x86_64/mod_func.o differ diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/naf_ms.c b/examples/example_models/str-dspn-model1/mechanisms/x86_64/naf_ms.c new file mode 100644 index 00000000..a1ecbd85 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/x86_64/naf_ms.c @@ -0,0 +1,702 @@ +/* Created by Language version: 7.7.0 */ +/* VECTORIZED */ +#define NRN_VECTORIZED 1 +#include +#include +#include +#include "mech_api.h" +#undef PI +#define nil 0 +#include "md1redef.h" +#include "section.h" +#include "nrniv_mf.h" +#include "md2redef.h" + +#if METHOD3 +extern int _method3; +#endif + +#if !NRNGPU +#undef exp +#define exp hoc_Exp +extern double hoc_Exp(double); +#endif + +#define nrn_init _nrn_init__naf_ms +#define _nrn_initial _nrn_initial__naf_ms +#define nrn_cur _nrn_cur__naf_ms +#define _nrn_current _nrn_current__naf_ms +#define nrn_jacob _nrn_jacob__naf_ms +#define nrn_state _nrn_state__naf_ms +#define _net_receive _net_receive__naf_ms +#define rates rates__naf_ms +#define states states__naf_ms + +#define _threadargscomma_ _p, _ppvar, _thread, _nt, +#define _threadargsprotocomma_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, +#define _threadargs_ _p, _ppvar, _thread, _nt +#define _threadargsproto_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt + /*SUPPRESS 761*/ + /*SUPPRESS 762*/ + /*SUPPRESS 763*/ + /*SUPPRESS 765*/ + extern double *getarg(); + /* Thread safe. No static _p or _ppvar. */ + +#define t _nt->_t +#define dt _nt->_dt +#define gbar _p[0] +#define gbar_columnindex 0 +#define modDA _p[1] +#define modDA_columnindex 1 +#define maxModDA _p[2] +#define maxModDA_columnindex 2 +#define levelDA _p[3] +#define levelDA_columnindex 3 +#define modACh _p[4] +#define modACh_columnindex 4 +#define maxModACh _p[5] +#define maxModACh_columnindex 5 +#define levelACh _p[6] +#define levelACh_columnindex 6 +#define ina _p[7] +#define ina_columnindex 7 +#define gna _p[8] +#define gna_columnindex 8 +#define m _p[9] +#define m_columnindex 9 +#define h _p[10] +#define h_columnindex 10 +#define ena _p[11] +#define ena_columnindex 11 +#define minf _p[12] +#define minf_columnindex 12 +#define mtau _p[13] +#define mtau_columnindex 13 +#define hinf _p[14] +#define hinf_columnindex 14 +#define htau _p[15] +#define htau_columnindex 15 +#define Dm _p[16] +#define Dm_columnindex 16 +#define Dh _p[17] +#define Dh_columnindex 17 +#define v _p[18] +#define v_columnindex 18 +#define _g _p[19] +#define _g_columnindex 19 +#define _ion_ena *_ppvar[0]._pval +#define _ion_ina *_ppvar[1]._pval +#define _ion_dinadv *_ppvar[2]._pval + +#if MAC +#if !defined(v) +#define v _mlhv +#endif +#if !defined(h) +#define h _mlhh +#endif +#endif + +#if defined(__cplusplus) +extern "C" { +#endif + static int hoc_nrnpointerindex = -1; + static Datum* _extcall_thread; + static Prop* _extcall_prop; + /* external NEURON variables */ + /* declaration of user functions */ + static void _hoc_modulationACh(void); + static void _hoc_modulationDA(void); + static void _hoc_rates(void); + static int _mechtype; +extern void _nrn_cacheloop_reg(int, int); +extern void hoc_register_prop_size(int, int, int); +extern void hoc_register_limits(int, HocParmLimits*); +extern void hoc_register_units(int, HocParmUnits*); +extern void nrn_promote(Prop*, int, int); +extern Memb_func* memb_func; + +#define NMODL_TEXT 1 +#if NMODL_TEXT +static const char* nmodl_file_text; +static const char* nmodl_filename; +extern void hoc_reg_nmodl_text(int, const char*); +extern void hoc_reg_nmodl_filename(int, const char*); +#endif + + extern void _nrn_setdata_reg(int, void(*)(Prop*)); + static void _setdata(Prop* _prop) { + _extcall_prop = _prop; + } + static void _hoc_setdata() { + Prop *_prop, *hoc_getdata_range(int); + _prop = hoc_getdata_range(_mechtype); + _setdata(_prop); + hoc_retpushx(1.); +} + /* connect user functions to hoc names */ + static VoidFunc hoc_intfunc[] = { + "setdata_naf_ms", _hoc_setdata, + "modulationACh_naf_ms", _hoc_modulationACh, + "modulationDA_naf_ms", _hoc_modulationDA, + "rates_naf_ms", _hoc_rates, + 0, 0 +}; +#define modulationACh modulationACh_naf_ms +#define modulationDA modulationDA_naf_ms + extern double modulationACh( _threadargsproto_ ); + extern double modulationDA( _threadargsproto_ ); + /* declare global and static user variables */ +#define q q_naf_ms + double q = 1.8; + /* some parameters have upper and lower limits */ + static HocParmLimits _hoc_parm_limits[] = { + 0,0,0 +}; + static HocParmUnits _hoc_parm_units[] = { + "gbar_naf_ms", "S/cm2", + "ina_naf_ms", "mA/cm2", + "gna_naf_ms", "S/cm2", + 0,0 +}; + static double delta_t = 0.01; + static double h0 = 0; + static double m0 = 0; + /* connect global user variables to hoc */ + static DoubScal hoc_scdoub[] = { + "q_naf_ms", &q_naf_ms, + 0,0 +}; + static DoubVec hoc_vdoub[] = { + 0,0,0 +}; + static double _sav_indep; + static void nrn_alloc(Prop*); +static void nrn_init(NrnThread*, _Memb_list*, int); +static void nrn_state(NrnThread*, _Memb_list*, int); + static void nrn_cur(NrnThread*, _Memb_list*, int); +static void nrn_jacob(NrnThread*, _Memb_list*, int); + +static int _ode_count(int); +static void _ode_map(int, double**, double**, double*, Datum*, double*, int); +static void _ode_spec(NrnThread*, _Memb_list*, int); +static void _ode_matsol(NrnThread*, _Memb_list*, int); + +#define _cvode_ieq _ppvar[3]._i + static void _ode_matsol_instance1(_threadargsproto_); + /* connect range variables in _p that hoc is supposed to know about */ + static const char *_mechanism[] = { + "7.7.0", +"naf_ms", + "gbar_naf_ms", + "modDA_naf_ms", + "maxModDA_naf_ms", + "levelDA_naf_ms", + "modACh_naf_ms", + "maxModACh_naf_ms", + "levelACh_naf_ms", + 0, + "ina_naf_ms", + "gna_naf_ms", + 0, + "m_naf_ms", + "h_naf_ms", + 0, + 0}; + static Symbol* _na_sym; + +extern Prop* need_memb(Symbol*); + +static void nrn_alloc(Prop* _prop) { + Prop *prop_ion; + double *_p; Datum *_ppvar; + _p = nrn_prop_data_alloc(_mechtype, 20, _prop); + /*initialize range parameters*/ + gbar = 0; + modDA = 0; + maxModDA = 1; + levelDA = 0; + modACh = 0; + maxModACh = 1; + levelACh = 0; + _prop->param = _p; + _prop->param_size = 20; + _ppvar = nrn_prop_datum_alloc(_mechtype, 4, _prop); + _prop->dparam = _ppvar; + /*connect ionic variables to this model*/ + prop_ion = need_memb(_na_sym); + nrn_promote(prop_ion, 0, 1); + _ppvar[0]._pval = &prop_ion->param[0]; /* ena */ + _ppvar[1]._pval = &prop_ion->param[3]; /* ina */ + _ppvar[2]._pval = &prop_ion->param[4]; /* _ion_dinadv */ + +} + static void _initlists(); + /* some states have an absolute tolerance */ + static Symbol** _atollist; + static HocStateTolerance _hoc_state_tol[] = { + 0,0 +}; + static void _update_ion_pointer(Datum*); + extern Symbol* hoc_lookup(const char*); +extern void _nrn_thread_reg(int, int, void(*)(Datum*)); +extern void _nrn_thread_table_reg(int, void(*)(double*, Datum*, Datum*, NrnThread*, int)); +extern void hoc_register_tolerance(int, HocStateTolerance*, Symbol***); +extern void _cvode_abstol( Symbol**, double*, int); + + void _naf_ms_reg() { + int _vectorized = 1; + _initlists(); + ion_reg("na", -10000.); + _na_sym = hoc_lookup("na_ion"); + register_mech(_mechanism, nrn_alloc,nrn_cur, nrn_jacob, nrn_state, nrn_init, hoc_nrnpointerindex, 1); + _mechtype = nrn_get_mechtype(_mechanism[1]); + _nrn_setdata_reg(_mechtype, _setdata); + _nrn_thread_reg(_mechtype, 2, _update_ion_pointer); + #if NMODL_TEXT + hoc_reg_nmodl_text(_mechtype, nmodl_file_text); + hoc_reg_nmodl_filename(_mechtype, nmodl_filename); +#endif + hoc_register_prop_size(_mechtype, 20, 4); + hoc_register_dparam_semantics(_mechtype, 0, "na_ion"); + hoc_register_dparam_semantics(_mechtype, 1, "na_ion"); + hoc_register_dparam_semantics(_mechtype, 2, "na_ion"); + hoc_register_dparam_semantics(_mechtype, 3, "cvodeieq"); + hoc_register_cvode(_mechtype, _ode_count, _ode_map, _ode_spec, _ode_matsol); + hoc_register_tolerance(_mechtype, _hoc_state_tol, &_atollist); + hoc_register_var(hoc_scdoub, hoc_vdoub, hoc_intfunc); + ivoc_help("help ?1 naf_ms /home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/naf_ms.mod\n"); + hoc_register_limits(_mechtype, _hoc_parm_limits); + hoc_register_units(_mechtype, _hoc_parm_units); + } +static int _reset; +static char *modelname = "Fast transient sodium current"; + +static int error; +static int _ninits = 0; +static int _match_recurse=1; +static void _modl_cleanup(){ _match_recurse=1;} +static int rates(_threadargsproto_); + +static int _ode_spec1(_threadargsproto_); +/*static int _ode_matsol1(_threadargsproto_);*/ + static int _slist1[2], _dlist1[2]; + static int states(_threadargsproto_); + +/*CVODE*/ + static int _ode_spec1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) {int _reset = 0; { + rates ( _threadargs_ ) ; + Dm = ( minf - m ) / mtau * q ; + Dh = ( hinf - h ) / htau * q ; + } + return _reset; +} + static int _ode_matsol1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + rates ( _threadargs_ ) ; + Dm = Dm / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) )) ; + Dh = Dh / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / htau )*( q ) )) ; + return 0; +} + /*END CVODE*/ + static int states (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { { + rates ( _threadargs_ ) ; + m = m + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / mtau )*( q ))))*(- ( ( ( ( minf ) ) / mtau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / mtau )*( q ) ) - m) ; + h = h + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / htau )*( q ))))*(- ( ( ( ( hinf ) ) / htau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / htau )*( q ) ) - h) ; + } + return 0; +} + +static int rates ( _threadargsproto_ ) { + minf = 1.0 / ( 1.0 + exp ( ( v - ( - 25.0 ) ) / ( - 10.0 ) ) ) ; + mtau = 0.33 + 1.0 / ( exp ( ( v - ( - 62.0 ) ) / 14.0 ) + exp ( ( v - ( - 60.0 ) ) / ( - 17.0 ) ) ) ; + hinf = 1.0 / ( 1.0 + exp ( ( v - ( - 62.0 ) ) / 6.0 ) ) ; + htau = 0.6 + 1.0 / ( exp ( ( v - ( - 44.0 ) ) / 8.0 ) + exp ( ( v - ( - 99.0 ) ) / ( - 44.0 ) ) ) ; + return 0; } + +static void _hoc_rates(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = 1.; + rates ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +double modulationDA ( _threadargsproto_ ) { + double _lmodulationDA; + _lmodulationDA = 1.0 + modDA * ( maxModDA - 1.0 ) * levelDA ; + +return _lmodulationDA; + } + +static void _hoc_modulationDA(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = modulationDA ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +double modulationACh ( _threadargsproto_ ) { + double _lmodulationACh; + _lmodulationACh = 1.0 + modACh * ( maxModACh - 1.0 ) * levelACh ; + +return _lmodulationACh; + } + +static void _hoc_modulationACh(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = modulationACh ( _p, _ppvar, _thread, _nt ); + hoc_retpushx(_r); +} + +static int _ode_count(int _type){ return 2;} + +static void _ode_spec(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + ena = _ion_ena; + _ode_spec1 (_p, _ppvar, _thread, _nt); + }} + +static void _ode_map(int _ieq, double** _pv, double** _pvdot, double* _pp, Datum* _ppd, double* _atol, int _type) { + double* _p; Datum* _ppvar; + int _i; _p = _pp; _ppvar = _ppd; + _cvode_ieq = _ieq; + for (_i=0; _i < 2; ++_i) { + _pv[_i] = _pp + _slist1[_i]; _pvdot[_i] = _pp + _dlist1[_i]; + _cvode_abstol(_atollist, _atol, _i); + } + } + +static void _ode_matsol_instance1(_threadargsproto_) { + _ode_matsol1 (_p, _ppvar, _thread, _nt); + } + +static void _ode_matsol(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + ena = _ion_ena; + _ode_matsol_instance1(_threadargs_); + }} + extern void nrn_update_ion_pointer(Symbol*, Datum*, int, int); + static void _update_ion_pointer(Datum* _ppvar) { + nrn_update_ion_pointer(_na_sym, _ppvar, 0, 0); + nrn_update_ion_pointer(_na_sym, _ppvar, 1, 3); + nrn_update_ion_pointer(_na_sym, _ppvar, 2, 4); + } + +static void initmodel(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + int _i; double _save;{ + h = h0; + m = m0; + { + rates ( _threadargs_ ) ; + m = minf ; + h = hinf ; + } + +} +} + +static void nrn_init(NrnThread* _nt, _Memb_list* _ml, int _type){ +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v = _v; + ena = _ion_ena; + initmodel(_p, _ppvar, _thread, _nt); + } +} + +static double _nrn_current(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, double _v){double _current=0.;v=_v;{ { + gna = gbar * m * m * m * h * modulationDA ( _threadargs_ ) * modulationACh ( _threadargs_ ) ; + ina = gna * ( v - ena ) ; + } + _current += ina; + +} return _current; +} + +static void nrn_cur(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; double _rhs, _v; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + ena = _ion_ena; + _g = _nrn_current(_p, _ppvar, _thread, _nt, _v + .001); + { double _dina; + _dina = ina; + _rhs = _nrn_current(_p, _ppvar, _thread, _nt, _v); + _ion_dinadv += (_dina - ina)/.001 ; + } + _g = (_g - _rhs)/.001; + _ion_ina += ina ; +#if CACHEVEC + if (use_cachevec) { + VEC_RHS(_ni[_iml]) -= _rhs; + }else +#endif + { + NODERHS(_nd) -= _rhs; + } + +} + +} + +static void nrn_jacob(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; +#if CACHEVEC + if (use_cachevec) { + VEC_D(_ni[_iml]) += _g; + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + NODED(_nd) += _g; + } + +} + +} + +static void nrn_state(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v = 0.0; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v=_v; +{ + ena = _ion_ena; + { states(_p, _ppvar, _thread, _nt); + } }} + +} + +static void terminal(){} + +static void _initlists(){ + double _x; double* _p = &_x; + int _i; static int _first = 1; + if (!_first) return; + _slist1[0] = m_columnindex; _dlist1[0] = Dm_columnindex; + _slist1[1] = h_columnindex; _dlist1[1] = Dh_columnindex; +_first = 0; +} + +#if defined(__cplusplus) +} /* extern "C" */ +#endif + +#if NMODL_TEXT +static const char* nmodl_filename = "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/naf_ms.mod"; +static const char* nmodl_file_text = + "TITLE Fast transient sodium current\n" + "\n" + "COMMENT\n" + "\n" + "Neuromodulation is added as functions:\n" + " \n" + " modulationDA = 1 + modDA*(maxModDA-1)*levelDA\n" + "\n" + "where:\n" + " \n" + " modDA [0]: is a switch for turning modulation on or off {1/0}\n" + " maxModDA [1]: is the maximum modulation for this specific channel (read from the param file)\n" + " e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf}\n" + " levelDA [0]: is an additional parameter for scaling modulation. \n" + " Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1}\n" + " \n" + " Further neuromodulators can be added by for example:\n" + " modulationDA = 1 + modDA*(maxModDA-1)\n" + " modulationACh = 1 + modACh*(maxModACh-1)\n" + " ....\n" + "\n" + " etc. for other neuromodulators\n" + " \n" + " \n" + " \n" + "[] == default values\n" + "{} == ranges\n" + "\n" + "ENDCOMMENT\n" + "\n" + "NEURON {\n" + " SUFFIX naf_ms\n" + " USEION na READ ena WRITE ina\n" + " RANGE gbar, gna, ina\n" + " RANGE modDA, maxModDA, levelDA\n" + " RANGE modACh, maxModACh, levelACh\n" + "}\n" + "\n" + "UNITS {\n" + " (S) = (siemens)\n" + " (mV) = (millivolt)\n" + " (mA) = (milliamp)\n" + "}\n" + "\n" + "PARAMETER {\n" + " gbar = 0.0 (S/cm2) \n" + " :q = 1 : room temperature 22 C\n" + " q = 1.8 : body temperature 35 C\n" + " modDA = 0\n" + " maxModDA = 1\n" + " levelDA = 0\n" + " modACh = 0\n" + " maxModACh = 1 \n" + " levelACh = 0\n" + "}\n" + "\n" + "ASSIGNED {\n" + " v (mV)\n" + " ena (mV)\n" + " ina (mA/cm2)\n" + " gna (S/cm2)\n" + " minf\n" + " mtau (ms)\n" + " hinf\n" + " htau (ms)\n" + "}\n" + "\n" + "STATE { m h }\n" + "\n" + "BREAKPOINT {\n" + " SOLVE states METHOD cnexp\n" + " gna = gbar*m*m*m*h*modulationDA()*modulationACh()\n" + " ina = gna*(v-ena)\n" + "}\n" + "\n" + "DERIVATIVE states {\n" + " rates()\n" + " m' = (minf-m)/mtau*q\n" + " h' = (hinf-h)/htau*q\n" + "}\n" + "\n" + "INITIAL {\n" + " rates()\n" + " m = minf\n" + " h = hinf\n" + "}\n" + "\n" + "PROCEDURE rates() {\n" + " UNITSOFF\n" + " :minf = 1/(1+exp((v-(-25.5))/(-9.2)))\n" + " :mtau = 0.33+1/(exp((v-(-62))/14)+exp((v-(-60))/(-17)))\n" + " :hinf = 1/(1+exp((v-(-63.2))/6))\n" + " :htau = 0.6+1/(exp((v-(-44))/8)+exp((v-(-99))/(-44)))\n" + " minf = 1/(1+exp((v-(-25))/(-10)))\n" + " mtau = 0.33+1/(exp((v-(-62))/14)+exp((v-(-60))/(-17)))\n" + " hinf = 1/(1+exp((v-(-62))/6))\n" + " htau = 0.6+1/(exp((v-(-44))/8)+exp((v-(-99))/(-44)))\n" + " UNITSON\n" + "}\n" + "\n" + "FUNCTION modulationDA() {\n" + " : returns modulation factor\n" + " \n" + " modulationDA = 1 + modDA*(maxModDA-1)*levelDA \n" + "}\n" + "\n" + "FUNCTION modulationACh() {\n" + " : returns modulation factor\n" + " \n" + " modulationACh = 1 + modACh*(maxModACh-1)*levelACh \n" + "}\n" + "\n" + "COMMENT\n" + "\n" + "Original data by Ogata and Tatebayashi (1990) [1]. Neostriatal neurons\n" + "of medium size (putative medium spiny neurons) freshly isolated from\n" + "the adult guinea pig brain (either sex, 200 g). Data compensated for\n" + "the liquid junction potential (-13 mV). Experiments carried out at room\n" + "temperature (22 C). Conductance fitted by m3h kinetics.\n" + "\n" + "Smooth fit of mtau and htau data [1] by Alexander Kozlov \n" + "assuming natural logarithm of tau values [1, Figs. 5 and 9] and\n" + "temperature correction factor of 1.8 [2] as suggested by Robert Lindroos\n" + ".\n" + "\n" + "[1] Ogata N, Tatebayashi H (1990) Sodium current kinetics in freshly\n" + "isolated neostriatal neurones of the adult guinea pig. Pflugers Arch\n" + "416(5):594-603.\n" + "\n" + "[2] Schwarz JR (1986) The effect of temperature on Na currents in rat\n" + "myelinated nerve fibres. Pflugers Arch. 406(4):397-404.\n" + "\n" + "ENDCOMMENT\n" + ; +#endif diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/naf_ms.o b/examples/example_models/str-dspn-model1/mechanisms/x86_64/naf_ms.o new file mode 100644 index 00000000..537a2d45 Binary files /dev/null and b/examples/example_models/str-dspn-model1/mechanisms/x86_64/naf_ms.o differ diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/sk_ms.c b/examples/example_models/str-dspn-model1/mechanisms/x86_64/sk_ms.c new file mode 100644 index 00000000..27c2aae7 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/x86_64/sk_ms.c @@ -0,0 +1,581 @@ +/* Created by Language version: 7.7.0 */ +/* VECTORIZED */ +#define NRN_VECTORIZED 1 +#include +#include +#include +#include "mech_api.h" +#undef PI +#define nil 0 +#include "md1redef.h" +#include "section.h" +#include "nrniv_mf.h" +#include "md2redef.h" + +#if METHOD3 +extern int _method3; +#endif + +#if !NRNGPU +#undef exp +#define exp hoc_Exp +extern double hoc_Exp(double); +#endif + +#define nrn_init _nrn_init__sk_ms +#define _nrn_initial _nrn_initial__sk_ms +#define nrn_cur _nrn_cur__sk_ms +#define _nrn_current _nrn_current__sk_ms +#define nrn_jacob _nrn_jacob__sk_ms +#define nrn_state _nrn_state__sk_ms +#define _net_receive _net_receive__sk_ms +#define rate rate__sk_ms +#define state state__sk_ms + +#define _threadargscomma_ _p, _ppvar, _thread, _nt, +#define _threadargsprotocomma_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, +#define _threadargs_ _p, _ppvar, _thread, _nt +#define _threadargsproto_ double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt + /*SUPPRESS 761*/ + /*SUPPRESS 762*/ + /*SUPPRESS 763*/ + /*SUPPRESS 765*/ + extern double *getarg(); + /* Thread safe. No static _p or _ppvar. */ + +#define t _nt->_t +#define dt _nt->_dt +#define gbar _p[0] +#define gbar_columnindex 0 +#define ik _p[1] +#define ik_columnindex 1 +#define o _p[2] +#define o_columnindex 2 +#define cai _p[3] +#define cai_columnindex 3 +#define ek _p[4] +#define ek_columnindex 4 +#define oinf _p[5] +#define oinf_columnindex 5 +#define otau _p[6] +#define otau_columnindex 6 +#define Do _p[7] +#define Do_columnindex 7 +#define v _p[8] +#define v_columnindex 8 +#define _g _p[9] +#define _g_columnindex 9 +#define _ion_cai *_ppvar[0]._pval +#define _ion_ek *_ppvar[1]._pval +#define _ion_ik *_ppvar[2]._pval +#define _ion_dikdv *_ppvar[3]._pval + +#if MAC +#if !defined(v) +#define v _mlhv +#endif +#if !defined(h) +#define h _mlhh +#endif +#endif + +#if defined(__cplusplus) +extern "C" { +#endif + static int hoc_nrnpointerindex = -1; + static Datum* _extcall_thread; + static Prop* _extcall_prop; + /* external NEURON variables */ + /* declaration of user functions */ + static void _hoc_rate(void); + static int _mechtype; +extern void _nrn_cacheloop_reg(int, int); +extern void hoc_register_prop_size(int, int, int); +extern void hoc_register_limits(int, HocParmLimits*); +extern void hoc_register_units(int, HocParmUnits*); +extern void nrn_promote(Prop*, int, int); +extern Memb_func* memb_func; + +#define NMODL_TEXT 1 +#if NMODL_TEXT +static const char* nmodl_file_text; +static const char* nmodl_filename; +extern void hoc_reg_nmodl_text(int, const char*); +extern void hoc_reg_nmodl_filename(int, const char*); +#endif + + extern void _nrn_setdata_reg(int, void(*)(Prop*)); + static void _setdata(Prop* _prop) { + _extcall_prop = _prop; + } + static void _hoc_setdata() { + Prop *_prop, *hoc_getdata_range(int); + _prop = hoc_getdata_range(_mechtype); + _setdata(_prop); + hoc_retpushx(1.); +} + /* connect user functions to hoc names */ + static VoidFunc hoc_intfunc[] = { + "setdata_sk_ms", _hoc_setdata, + "rate_sk_ms", _hoc_rate, + 0, 0 +}; + /* declare global and static user variables */ +#define q q_sk_ms + double q = 1; + /* some parameters have upper and lower limits */ + static HocParmLimits _hoc_parm_limits[] = { + 0,0,0 +}; + static HocParmUnits _hoc_parm_units[] = { + "gbar_sk_ms", "mho/cm2", + "ik_sk_ms", "mA/cm2", + 0,0 +}; + static double delta_t = 0.01; + static double o0 = 0; + /* connect global user variables to hoc */ + static DoubScal hoc_scdoub[] = { + "q_sk_ms", &q_sk_ms, + 0,0 +}; + static DoubVec hoc_vdoub[] = { + 0,0,0 +}; + static double _sav_indep; + static void nrn_alloc(Prop*); +static void nrn_init(NrnThread*, _Memb_list*, int); +static void nrn_state(NrnThread*, _Memb_list*, int); + static void nrn_cur(NrnThread*, _Memb_list*, int); +static void nrn_jacob(NrnThread*, _Memb_list*, int); + +static int _ode_count(int); +static void _ode_map(int, double**, double**, double*, Datum*, double*, int); +static void _ode_spec(NrnThread*, _Memb_list*, int); +static void _ode_matsol(NrnThread*, _Memb_list*, int); + +#define _cvode_ieq _ppvar[4]._i + static void _ode_matsol_instance1(_threadargsproto_); + /* connect range variables in _p that hoc is supposed to know about */ + static const char *_mechanism[] = { + "7.7.0", +"sk_ms", + "gbar_sk_ms", + 0, + "ik_sk_ms", + 0, + "o_sk_ms", + 0, + 0}; + static Symbol* _ca_sym; + static Symbol* _k_sym; + +extern Prop* need_memb(Symbol*); + +static void nrn_alloc(Prop* _prop) { + Prop *prop_ion; + double *_p; Datum *_ppvar; + _p = nrn_prop_data_alloc(_mechtype, 10, _prop); + /*initialize range parameters*/ + gbar = 0; + _prop->param = _p; + _prop->param_size = 10; + _ppvar = nrn_prop_datum_alloc(_mechtype, 5, _prop); + _prop->dparam = _ppvar; + /*connect ionic variables to this model*/ + prop_ion = need_memb(_ca_sym); + nrn_promote(prop_ion, 1, 0); + _ppvar[0]._pval = &prop_ion->param[1]; /* cai */ + prop_ion = need_memb(_k_sym); + nrn_promote(prop_ion, 0, 1); + _ppvar[1]._pval = &prop_ion->param[0]; /* ek */ + _ppvar[2]._pval = &prop_ion->param[3]; /* ik */ + _ppvar[3]._pval = &prop_ion->param[4]; /* _ion_dikdv */ + +} + static void _initlists(); + /* some states have an absolute tolerance */ + static Symbol** _atollist; + static HocStateTolerance _hoc_state_tol[] = { + 0,0 +}; + static void _update_ion_pointer(Datum*); + extern Symbol* hoc_lookup(const char*); +extern void _nrn_thread_reg(int, int, void(*)(Datum*)); +extern void _nrn_thread_table_reg(int, void(*)(double*, Datum*, Datum*, NrnThread*, int)); +extern void hoc_register_tolerance(int, HocStateTolerance*, Symbol***); +extern void _cvode_abstol( Symbol**, double*, int); + + void _sk_ms_reg() { + int _vectorized = 1; + _initlists(); + ion_reg("ca", -10000.); + ion_reg("k", -10000.); + _ca_sym = hoc_lookup("ca_ion"); + _k_sym = hoc_lookup("k_ion"); + register_mech(_mechanism, nrn_alloc,nrn_cur, nrn_jacob, nrn_state, nrn_init, hoc_nrnpointerindex, 1); + _mechtype = nrn_get_mechtype(_mechanism[1]); + _nrn_setdata_reg(_mechtype, _setdata); + _nrn_thread_reg(_mechtype, 2, _update_ion_pointer); + #if NMODL_TEXT + hoc_reg_nmodl_text(_mechtype, nmodl_file_text); + hoc_reg_nmodl_filename(_mechtype, nmodl_filename); +#endif + hoc_register_prop_size(_mechtype, 10, 5); + hoc_register_dparam_semantics(_mechtype, 0, "ca_ion"); + hoc_register_dparam_semantics(_mechtype, 1, "k_ion"); + hoc_register_dparam_semantics(_mechtype, 2, "k_ion"); + hoc_register_dparam_semantics(_mechtype, 3, "k_ion"); + hoc_register_dparam_semantics(_mechtype, 4, "cvodeieq"); + hoc_register_cvode(_mechtype, _ode_count, _ode_map, _ode_spec, _ode_matsol); + hoc_register_tolerance(_mechtype, _hoc_state_tol, &_atollist); + hoc_register_var(hoc_scdoub, hoc_vdoub, hoc_intfunc); + ivoc_help("help ?1 sk_ms /home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/sk_ms.mod\n"); + hoc_register_limits(_mechtype, _hoc_parm_limits); + hoc_register_units(_mechtype, _hoc_parm_units); + } +static int _reset; +static char *modelname = "SK-type calcium activated K channel (KCa2.2)"; + +static int error; +static int _ninits = 0; +static int _match_recurse=1; +static void _modl_cleanup(){ _match_recurse=1;} +static int rate(_threadargsprotocomma_ double, double); + +static int _ode_spec1(_threadargsproto_); +/*static int _ode_matsol1(_threadargsproto_);*/ + static int _slist1[1], _dlist1[1]; + static int state(_threadargsproto_); + +/*CVODE*/ + static int _ode_spec1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) {int _reset = 0; { + rate ( _threadargscomma_ v , cai ) ; + Do = ( oinf - o ) / otau * q ; + } + return _reset; +} + static int _ode_matsol1 (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + rate ( _threadargscomma_ v , cai ) ; + Do = Do / (1. - dt*( ( ( ( ( - 1.0 ) ) ) / otau )*( q ) )) ; + return 0; +} + /*END CVODE*/ + static int state (double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { { + rate ( _threadargscomma_ v , cai ) ; + o = o + (1. - exp(dt*(( ( ( ( - 1.0 ) ) ) / otau )*( q ))))*(- ( ( ( ( oinf ) ) / otau )*( q ) ) / ( ( ( ( ( - 1.0 ) ) ) / otau )*( q ) ) - o) ; + } + return 0; +} + +static int rate ( _threadargsprotocomma_ double _lv , double _lca ) { + double _la ; + _la = pow( ( _lca / 0.57e-3 ) , 5.2 ) ; + oinf = _la / ( 1.0 + _la ) ; + otau = 4.9 ; + return 0; } + +static void _hoc_rate(void) { + double _r; + double* _p; Datum* _ppvar; Datum* _thread; NrnThread* _nt; + if (_extcall_prop) {_p = _extcall_prop->param; _ppvar = _extcall_prop->dparam;}else{ _p = (double*)0; _ppvar = (Datum*)0; } + _thread = _extcall_thread; + _nt = nrn_threads; + _r = 1.; + rate ( _p, _ppvar, _thread, _nt, *getarg(1) , *getarg(2) ); + hoc_retpushx(_r); +} + +static int _ode_count(int _type){ return 1;} + +static void _ode_spec(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + cai = _ion_cai; + ek = _ion_ek; + _ode_spec1 (_p, _ppvar, _thread, _nt); + }} + +static void _ode_map(int _ieq, double** _pv, double** _pvdot, double* _pp, Datum* _ppd, double* _atol, int _type) { + double* _p; Datum* _ppvar; + int _i; _p = _pp; _ppvar = _ppd; + _cvode_ieq = _ieq; + for (_i=0; _i < 1; ++_i) { + _pv[_i] = _pp + _slist1[_i]; _pvdot[_i] = _pp + _dlist1[_i]; + _cvode_abstol(_atollist, _atol, _i); + } + } + +static void _ode_matsol_instance1(_threadargsproto_) { + _ode_matsol1 (_p, _ppvar, _thread, _nt); + } + +static void _ode_matsol(NrnThread* _nt, _Memb_list* _ml, int _type) { + double* _p; Datum* _ppvar; Datum* _thread; + Node* _nd; double _v; int _iml, _cntml; + _cntml = _ml->_nodecount; + _thread = _ml->_thread; + for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; + v = NODEV(_nd); + cai = _ion_cai; + ek = _ion_ek; + _ode_matsol_instance1(_threadargs_); + }} + extern void nrn_update_ion_pointer(Symbol*, Datum*, int, int); + static void _update_ion_pointer(Datum* _ppvar) { + nrn_update_ion_pointer(_ca_sym, _ppvar, 0, 1); + nrn_update_ion_pointer(_k_sym, _ppvar, 1, 0); + nrn_update_ion_pointer(_k_sym, _ppvar, 2, 3); + nrn_update_ion_pointer(_k_sym, _ppvar, 3, 4); + } + +static void initmodel(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt) { + int _i; double _save;{ + o = o0; + { + rate ( _threadargscomma_ v , cai ) ; + o = oinf ; + } + +} +} + +static void nrn_init(NrnThread* _nt, _Memb_list* _ml, int _type){ +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v = _v; + cai = _ion_cai; + ek = _ion_ek; + initmodel(_p, _ppvar, _thread, _nt); + } +} + +static double _nrn_current(double* _p, Datum* _ppvar, Datum* _thread, NrnThread* _nt, double _v){double _current=0.;v=_v;{ { + ik = gbar * o * ( v - ek ) ; + } + _current += ik; + +} return _current; +} + +static void nrn_cur(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; double _rhs, _v; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + cai = _ion_cai; + ek = _ion_ek; + _g = _nrn_current(_p, _ppvar, _thread, _nt, _v + .001); + { double _dik; + _dik = ik; + _rhs = _nrn_current(_p, _ppvar, _thread, _nt, _v); + _ion_dikdv += (_dik - ik)/.001 ; + } + _g = (_g - _rhs)/.001; + _ion_ik += ik ; +#if CACHEVEC + if (use_cachevec) { + VEC_RHS(_ni[_iml]) -= _rhs; + }else +#endif + { + NODERHS(_nd) -= _rhs; + } + +} + +} + +static void nrn_jacob(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; +#if CACHEVEC + if (use_cachevec) { + VEC_D(_ni[_iml]) += _g; + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + NODED(_nd) += _g; + } + +} + +} + +static void nrn_state(NrnThread* _nt, _Memb_list* _ml, int _type) { +double* _p; Datum* _ppvar; Datum* _thread; +Node *_nd; double _v = 0.0; int* _ni; int _iml, _cntml; +#if CACHEVEC + _ni = _ml->_nodeindices; +#endif +_cntml = _ml->_nodecount; +_thread = _ml->_thread; +for (_iml = 0; _iml < _cntml; ++_iml) { + _p = _ml->_data[_iml]; _ppvar = _ml->_pdata[_iml]; + _nd = _ml->_nodelist[_iml]; +#if CACHEVEC + if (use_cachevec) { + _v = VEC_V(_ni[_iml]); + }else +#endif + { + _nd = _ml->_nodelist[_iml]; + _v = NODEV(_nd); + } + v=_v; +{ + cai = _ion_cai; + ek = _ion_ek; + { state(_p, _ppvar, _thread, _nt); + } }} + +} + +static void terminal(){} + +static void _initlists(){ + double _x; double* _p = &_x; + int _i; static int _first = 1; + if (!_first) return; + _slist1[0] = o_columnindex; _dlist1[0] = Do_columnindex; +_first = 0; +} + +#if defined(__cplusplus) +} /* extern "C" */ +#endif + +#if NMODL_TEXT +static const char* nmodl_filename = "/home/HDD-drive/Repos/Alex_model_repo/models/optim/HBP-2022Q2/str-dspn-e150602_c1_D1-mWT-0728MSN01-v20220620/mechanisms/sk_ms.mod"; +static const char* nmodl_file_text = + "TITLE SK-type calcium activated K channel (KCa2.2)\n" + "\n" + "UNITS {\n" + " (molar) = (1/liter)\n" + " (mV) = (millivolt)\n" + " (mA) = (milliamp)\n" + " (mM) = (millimolar)\n" + "}\n" + "\n" + "NEURON {\n" + " SUFFIX sk_ms\n" + " USEION ca READ cai\n" + " USEION k READ ek WRITE ik\n" + " RANGE gbar, ik\n" + "}\n" + "\n" + "PARAMETER {\n" + " gbar = 0.0 (mho/cm2)\n" + " :q = 1 : room temperature\n" + " q = 1 : body temperature\n" + "}\n" + "\n" + "ASSIGNED {\n" + " v (mV)\n" + " ik (mA/cm2)\n" + " cai (mM) \n" + " ek (mV)\n" + " oinf\n" + " otau (ms)\n" + "}\n" + "\n" + "STATE { o }\n" + "\n" + "BREAKPOINT {\n" + " SOLVE state METHOD cnexp\n" + " ik = gbar*o*(v-ek)\n" + "}\n" + "\n" + "DERIVATIVE state {\n" + " rate(v, cai)\n" + " o' = (oinf-o)/otau*q\n" + "}\n" + "\n" + "INITIAL {\n" + " rate(v, cai)\n" + " o = oinf\n" + "}\n" + "\n" + "PROCEDURE rate(v (mV), ca (mM)) {\n" + " LOCAL a\n" + " a = (ca/0.57e-3)^5.2\n" + " oinf = a/(1+a)\n" + " otau = 4.9\n" + "}\n" + "\n" + "COMMENT\n" + "\n" + "Experimental data was obtained for the apamin-sensitive clone rSK2 from\n" + "rat brain cDNA expressed in Xenopus oocytes [1,2]. All experiments were\n" + "performed at room tempretaure.\n" + "\n" + "Original model [3] used calcium dependence from [2, Fig.2] and calcium\n" + "activation time constant from [1, Fig.13]. NEURON implementation by\n" + "Alexander Kozlov follows the revised model [4].\n" + "\n" + "[1] Hirschberg B, Maylie J, Adelman JP, Marrion NV (1998) Gating of\n" + "recombinant small-conductance Ca-activated K+ channels by calcium. J\n" + "Gen Physiol 111(4):565-81.\n" + "\n" + "[2] Maylie J, Bond CT, Herson PS, Lee WS, Adelman JP (2004) Small\n" + "conductance Ca2+-activated K+ channels and calmodulin. J Physiol 554(Pt\n" + "2):255-61.\n" + "\n" + "[3] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH,\n" + "Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on\n" + "calcium influx and spike timing-dependent plasticity in striatal medium\n" + "spiny neurons. PLoS Comput Biol 8(4):e1002493.\n" + "\n" + "[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of\n" + "spike timing-dependent calcium influx during corticostriatal upstates. J\n" + "Neurophysiol 110(7):1631-45.\n" + "\n" + "ENDCOMMENT\n" + ; +#endif diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/sk_ms.o b/examples/example_models/str-dspn-model1/mechanisms/x86_64/sk_ms.o new file mode 100644 index 00000000..153b1bf7 Binary files /dev/null and b/examples/example_models/str-dspn-model1/mechanisms/x86_64/sk_ms.o differ diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/special b/examples/example_models/str-dspn-model1/mechanisms/x86_64/special new file mode 100755 index 00000000..12ab8be7 --- /dev/null +++ b/examples/example_models/str-dspn-model1/mechanisms/x86_64/special @@ -0,0 +1,123 @@ +#!/usr/bin/python3 +""" +A generic wrapper to access nrn binaries from a python installation +Please create a softlink with the binary name to be called. +""" +import os +import shutil +import subprocess +import sys +from pkg_resources import working_set +from setuptools.command.build_ext import new_compiler +from packaging.version import Version +from sysconfig import get_config_vars, get_config_var + + +def _customize_compiler(compiler): + """Do platform-sepcific customizations of compilers on unix platforms.""" + if compiler.compiler_type == "unix": + (cc, cxx, cflags) = get_config_vars("CC", "CXX", "CFLAGS") + if "CC" in os.environ: + cc = os.environ["CC"] + if "CXX" in os.environ: + cxx = os.environ["CXX"] + if "CFLAGS" in os.environ: + cflags = cflags + " " + os.environ["CFLAGS"] + cc_cmd = cc + " " + cflags + # We update executables in compiler to take advantage of distutils arg splitting + compiler.set_executables(compiler=cc_cmd, compiler_cxx=cxx) + + +def _set_default_compiler(): + """Set (dont overwrite) CC/CXX so that apps dont use the build-time ones""" + ccompiler = new_compiler() + _customize_compiler(ccompiler) + # xcrun wrapper must bring all args + if ccompiler.compiler[0] == "xcrun": + ccompiler.compiler[0] = get_config_var("CC") + ccompiler.compiler_cxx[0] = get_config_var("CXX") + os.environ.setdefault("CC", ccompiler.compiler[0]) + os.environ.setdefault("CXX", ccompiler.compiler_cxx[0]) + + +def _check_cpp_compiler_version(): + """Check if GCC compiler is >= 9.0 otherwise show warning""" + try: + cpp_compiler = os.environ.get("CXX", "") + version = subprocess.run( + [cpp_compiler, "--version"], stdout=subprocess.PIPE + ).stdout.decode("utf-8") + if "GCC" in version: + version = subprocess.run( + [cpp_compiler, "-dumpversion"], stdout=subprocess.PIPE + ).stdout.decode("utf-8") + if Version(version) <= Version("9.0"): + print( + "Warning: GCC >= 9.0 is required with this version of NEURON but found", + version, + ) + except: + pass + + +def _config_exe(exe_name): + """Sets the environment to run the real executable (returned)""" + + package_name = "neuron" + + # determine package to find the install location + if "neuron-gpu-nightly" in working_set.by_key: + print("INFO : Using neuron-gpu-nightly Package (Alpha Developer Version)") + package_name = "neuron-gpu-nightly" + elif "neuron-gpu" in working_set.by_key: + print("INFO : Using neuron-gpu Package (Alpha Version)") + package_name = "neuron-gpu" + elif "neuron-nightly" in working_set.by_key: + print("INFO : Using neuron-nightly Package (Developer Version)") + package_name = "neuron-nightly" + elif "neuron" in working_set.by_key: + package_name = "neuron" + else: + raise RuntimeError("NEURON package not found! Verify PYTHONPATH") + + NRN_PREFIX = os.path.join( + working_set.by_key[package_name].location, "neuron", ".data" + ) + os.environ["NEURONHOME"] = os.path.join(NRN_PREFIX, "share/nrn") + os.environ["NRNHOME"] = NRN_PREFIX + os.environ["CORENRNHOME"] = NRN_PREFIX + os.environ["NRN_PYTHONEXE"] = sys.executable + os.environ["CORENRN_PYTHONEXE"] = sys.executable + os.environ["CORENRN_PERLEXE"] = shutil.which("perl") + os.environ["NRNBIN"] = os.path.dirname(__file__) + + _set_default_compiler() + return os.path.join(NRN_PREFIX, "bin", exe_name) + + +def _wrap_executable(output_name): + """Create a wrapper for an executable in same dir. Requires renaming the original file. + Executables are typically found under arch_name + """ + release_dir = os.path.join(os.environ["NEURONHOME"], "demo/release") + arch_name = next(os.walk(release_dir))[1][0] # first dir + file_path = os.path.join(arch_name, output_name) + shutil.move(file_path, file_path + ".nrn") + shutil.copy(__file__, file_path) + + +if __name__ == "__main__": + exe = _config_exe(os.path.basename(sys.argv[0])) + + if exe.endswith("nrnivmodl"): + # To create a wrapper for special (so it also gets ENV vars) we intercept nrnivmodl + subprocess.check_call([exe, *sys.argv[1:]]) + _wrap_executable("special") + sys.exit(0) + + if exe.endswith("special"): + exe = os.path.join( + sys.argv[0] + ".nrn" + ) # original special is renamed special.nrn + + os.execv(exe, sys.argv) diff --git a/examples/example_models/str-dspn-model1/mechanisms/x86_64/special.nrn b/examples/example_models/str-dspn-model1/mechanisms/x86_64/special.nrn new file mode 100755 index 00000000..b13905ab 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b/examples/example_models/str-dspn-model1/morphology/WT-0728MSN01-cor-rep-ax-res3-var4.swc similarity index 100% rename from examples/example_models/dspn/str-dspn-model1/morphology/WT-0728MSN01-cor-rep-ax-res3-var4.swc rename to examples/example_models/str-dspn-model1/morphology/WT-0728MSN01-cor-rep-ax-res3-var4.swc diff --git a/examples/example_models/dspn/str-dspn-model1/morphology/WT-0728MSN01-cor-rep-ax-res3-var5.swc b/examples/example_models/str-dspn-model1/morphology/WT-0728MSN01-cor-rep-ax-res3-var5.swc similarity index 100% rename from examples/example_models/dspn/str-dspn-model1/morphology/WT-0728MSN01-cor-rep-ax-res3-var5.swc rename to examples/example_models/str-dspn-model1/morphology/WT-0728MSN01-cor-rep-ax-res3-var5.swc diff --git a/examples/example_models/dspn/str-dspn-model1/morphology/WT-0728MSN01-cor-rep-ax-res3-var6.swc b/examples/example_models/str-dspn-model1/morphology/WT-0728MSN01-cor-rep-ax-res3-var6.swc similarity index 100% rename from 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examples/example_models/str-dspn-model1/morphology/WT-0728MSN01-cor-rep-ax-res3-var8.swc diff --git a/examples/example_models/dspn/str-dspn-model1/morphology/WT-0728MSN01-cor-rep-ax-res3.swc b/examples/example_models/str-dspn-model1/morphology/WT-0728MSN01-cor-rep-ax-res3.swc similarity index 100% rename from examples/example_models/dspn/str-dspn-model1/morphology/WT-0728MSN01-cor-rep-ax-res3.swc rename to examples/example_models/str-dspn-model1/morphology/WT-0728MSN01-cor-rep-ax-res3.swc diff --git a/examples/example_models/str-dspn-model1/val_models.json b/examples/example_models/str-dspn-model1/val_models.json new file mode 100644 index 00000000..16eb67d6 --- /dev/null +++ b/examples/example_models/str-dspn-model1/val_models.json @@ -0,0 +1,3982 @@ +[ + { + "ephys": "150602_c1_D1", + "morph": "WT-0728MSN01-cor-rep-ax-res3-var0.swc", + "par": 3, + "iv_curve": { + "stim": [ + -397.6, + -387.50825688073394, + -377.4165137614679, + -367.3247706422019, + -357.2330275229358, + 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a/examples/example_models/str-dspn-model2/config/parameters.json b/examples/example_models/str-dspn-model2/config/parameters.json new file mode 100644 index 00000000..046701af --- /dev/null +++ b/examples/example_models/str-dspn-model2/config/parameters.json @@ -0,0 +1,334 @@ +[ + { + "param_name": "celsius", + "type": "global", + "value": 35 + }, + { + "param_name": "v_init", + "type": "global", + "value": -86 + }, + { + "param_name": "g_pas", + "sectionlist": "axonal", + "type": "section", + "dist_type": "uniform", + "bounds": [ 0.00001072315818062772 , 0.001072315818062772 ] + }, + { + "param_name": "g_pas", + "sectionlist": "basal", + "type": "section", + "dist_type": "uniform", + "bounds": [ 0.00001072315818062772 , 0.001072315818062772 ] + }, + { + "param_name": "g_pas", + "sectionlist": "somatic", + "type": "section", + "dist_type": "uniform", + "bounds": [ 0.00001072315818062772 , 0.001072315818062772 ] + }, + { + "param_name": "e_pas", + "sectionlist": "all", + "type": 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"dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "axonal" + }, + { + "param_name": "gbar_naf_ms", + "mech": "naf_ms", + "bounds": [0, 20] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "gbar_kaf_ms", + "mech": "kaf_ms", + "bounds": [0, 5.0] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "q_kaf_ms", + "mech": "kaf_ms", + "value": 1.2310764224556812 , + "dist_type": "uniform", + "mech_param": "q", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "gbar_kas_ms", + "mech": "kas_ms", + "bounds": [0, 0.1] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "gbar_kdr_ms", + "mech": "kdr_ms", + "bounds": [0, 0.001] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "gbar_kir_ms", + "mech": "kir_ms", + "bounds": [ 0.0007211933727647813 , 0.07211933727647813 ], + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "pbar_cal12_ms", + "mech": "cal12_ms", + "value": 1e-5 , + "dist_type": "uniform", + "mech_param": "pbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "pbar_cal13_ms", + "mech": "cal13_ms", + "value": 1e-6 , + "dist_type": "uniform", + "mech_param": "pbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "pbar_can_ms", + "mech": "can_ms", + "value": 3e-5 , + "dist_type": "uniform", + "mech_param": "pbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "pbar_car_ms", + "mech": "car_ms", + "value": 1e-4 , + "dist_type": "uniform", + "mech_param": "pbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "gbar_bk_ms", + "mech": "bk_ms", + "bounds": [0, 1e-4 ] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "gbar_sk_ms", + "mech": "sk_ms", + "bounds": [0, 1e-4 ] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "gbar_Im_ms", + "mech": "Im_ms", + "bounds": [1e-4, 1e-2] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "gbar_naf_ms", + "mech": "naf_ms", + "bounds": [0, 0.02] , + "dist": "((0.1) + (0.9)/(1 + exp(({distance}-(40))/(10))))*{value}", + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "basal" + }, + { + "param_name": "gbar_kaf_ms", + "mech": "kaf_ms", + "bounds": [0.002, 0.5] , + "dist": "((1.0) + (0.5)/(1 + exp(({distance}-(120))/(-30))))*{value}", + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "basal" + }, + { + "param_name": "q_kaf_ms", + "mech": "kaf_ms", + "value": 1.2310764224556812 , + "dist_type": "uniform", + "mech_param": "q", + "type": "range", + "sectionlist": "basal" + }, + { + "param_name": "gbar_kas_ms", + "mech": "kas_ms", + "bounds": [0, 0.01] , + "dist": "((1.0) + (9.0)*exp(({distance}-(0.0))/(-5.0)))*{value}", + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "basal" + }, + { + "param_name": "gbar_kdr_ms", + "mech": "kdr_ms", + "bounds": [0, 0.001] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "basal" + }, + { + "param_name": "gbar_kir_ms", + "mech": "kir_ms", + "bounds": [ 4.2009113125876394e-06 , 4.2009113125876394e-04 ], + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "basal" + }, + { + "param_name": "pbar_cal12_ms", + "mech": "cal12_ms", + "value": 1e-5 , + "dist_type": "uniform", + "mech_param": "pbar", + "type": "range", + "sectionlist": "basal" + }, + { + "param_name": "pbar_cal13_ms", + "mech": "cal13_ms", + "value": 1e-6 , + "dist_type": "uniform", + "mech_param": "pbar", + "type": "range", + "sectionlist": "basal" + }, + { + "param_name": "pbar_car_ms", + "mech": "car_ms", + "value": 1e-4 , + "dist_type": "uniform", + "mech_param": "pbar", + "type": "range", + "sectionlist": "basal" + }, + { + "param_name": "pbar_cat32_ms", + "mech": "cat32_ms", + "value": 0.2e-8 , + "dist": "((0.0) + (1.0)/(1 + exp(({distance}-(120))/(-30))))*{value}", + "dist_type": "uniform", + "mech_param": "pbar", + "type": "range", + "sectionlist": "basal" + }, + { + "param_name": "pbar_cat33_ms", + "mech": "cat33_ms", + "value": 0.2e-8 , + "dist": "((0.0) + (1.0)/(1 + exp(({distance}-(120))/(-30))))*{value}", + "dist_type": "uniform", + "mech_param": "pbar", + "type": "range", + "sectionlist": "basal" + }, + { + "param_name": "gbar_bk_ms", + "mech": "bk_ms", + "bounds": [0, 1e-5] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "basal" + }, + { + "param_name": "gbar_sk_ms", + "mech": "sk_ms", + "bounds": [0, 1e-5] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "basal" + } +] diff --git a/examples/example_models/str-dspn-model2/config/protocols.json b/examples/example_models/str-dspn-model2/config/protocols.json new file mode 100644 index 00000000..e25079ef --- /dev/null +++ b/examples/example_models/str-dspn-model2/config/protocols.json @@ -0,0 +1,98 @@ +{ + "IV_499": { + "stimuli": [ + { + "delay": 100.0, + "amp": -0.3151, + "duration": 1000.0, + "totduration": 1400.0 + }, + { + "delay": 0.0, + "amp": 0.0597, + "duration": 1400.0, + "totduration": 1400.0 + } + ] + }, + "IV_502": { + "stimuli": [ + { + "delay": 100.0, + "amp": -0.1578, + "duration": 1000.0, + "totduration": 1400.0 + }, + { + "delay": 0.0, + "amp": 0.0597, + "duration": 1400.0, + "totduration": 1400.0 + } + ] + }, + "IDthresh-sub_541": { + "stimuli": [ + { + "delay": 700.0, + "amp": 0.2676, + "duration": 2000.0, + "totduration": 3000.0 + }, + { + "delay": 0.0, + "amp": 0.0597, + "duration": 3000.0, + "totduration": 3000.0 + } + ] + }, + "IDthresh_543": { + "stimuli": [ + { + "delay": 700.0, + "amp": 0.283, + "duration": 2000.0, + "totduration": 3000.0 + }, + { + "delay": 0.0, + "amp": 0.0597, + "duration": 3000.0, + "totduration": 3000.0 + } + ] + }, + "IDthresh_544": { + "stimuli": [ + { + "delay": 700.0, + "amp": 0.2909, + "duration": 2000.0, + "totduration": 3000.0 + }, + { + "delay": 0.0, + "amp": 0.0597, + "duration": 3000.0, + "totduration": 3000.0 + } + ] + }, + "IDthresh_553": { + "stimuli": [ + { + "delay": 700.0, + "amp": 0.3616, + "duration": 2000.0, + "totduration": 3000.0 + }, + { + "delay": 0.0, + "amp": 0.0597, + "duration": 3000.0, + "totduration": 3000.0 + } + ] + } +} diff --git a/examples/example_models/str-dspn-model2/hall_of_fame.json b/examples/example_models/str-dspn-model2/hall_of_fame.json new file mode 100644 index 00000000..e3b84772 --- /dev/null +++ b/examples/example_models/str-dspn-model2/hall_of_fame.json @@ -0,0 +1,209 @@ +[ + { + "g_pas.axonal": 0.00046838203895392504, + "g_pas.basal": 2.6573479906519564e-05, + "g_pas.somatic": 0.0007940010789653632, + "gbar_Im_ms.axonal": 0.06081955466534622, + "gbar_Im_ms.somatic": 0.00020074002912092825, + "gbar_bk_ms.basal": 7.369484041619922e-06, + "gbar_bk_ms.somatic": 9.054688741542861e-06, + "gbar_kaf_ms.basal": 0.0021096686832104716, + "gbar_kaf_ms.somatic": 0.41395847391438684, + "gbar_kas_ms.axonal": 0.09788035435350273, + "gbar_kas_ms.basal": 0.0005446066555583089, + "gbar_kas_ms.somatic": 0.0002627329609965164, + "gbar_kdr_ms.basal": 3.50915367284958e-05, + "gbar_kdr_ms.somatic": 0.0007235326683580692, + "gbar_kir_ms.basal": 4.6077499979595485e-05, + "gbar_kir_ms.somatic": 0.0007215332100207627, + "gbar_naf_ms.axonal": 1.704135346370423, + "gbar_naf_ms.basal": 0.018976244326848295, + "gbar_naf_ms.somatic": 9.955724193646837, + "gbar_sk_ms.basal": 3.8921619035638306e-08, + 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"gbar_Im_ms.somatic": 0.00030753692640130876, + "gbar_bk_ms.basal": 7.731109511140667e-06, + "gbar_bk_ms.somatic": 1.3190477016408482e-05, + "gbar_kaf_ms.basal": 0.002096100147614842, + "gbar_kaf_ms.somatic": 0.7005744449453343, + "gbar_kas_ms.axonal": 0.009457523318060717, + "gbar_kas_ms.basal": 0.0002675028989419833, + "gbar_kas_ms.somatic": 0.002636756093497969, + "gbar_kdr_ms.basal": 4.7436385604402776e-05, + "gbar_kdr_ms.somatic": 0.0006686431370695903, + "gbar_kir_ms.basal": 4.335670386087016e-05, + "gbar_kir_ms.somatic": 0.0007609654766626511, + "gbar_naf_ms.axonal": 1.5901575637006713, + "gbar_naf_ms.basal": 0.017315045428486955, + "gbar_naf_ms.somatic": 11.090631287971188, + "gbar_sk_ms.basal": 4.5449708475924313e-07, + "gbar_sk_ms.somatic": 5.849268359149641e-05 + } +] \ No newline at end of file diff --git a/examples/example_models/str-dspn-model2/mechanisms/Im_ms.mod b/examples/example_models/str-dspn-model2/mechanisms/Im_ms.mod new file mode 100644 index 00000000..68313240 --- /dev/null +++ b/examples/example_models/str-dspn-model2/mechanisms/Im_ms.mod @@ -0,0 +1,104 @@ +COMMENT +Mechanism taken from Doron et al., 2017 +https://senselab.med.yale.edu/ModelDB/ShowModel.cshtml?model=231427&file=/reproduction/Im.mod#tabs-2 + +Reference : Adams et al. 1982 - M-currents and other potassium currents in bullfrog sympathetic neurones + +corrected rates using q10 = 2.3, target temperature 34, orginal 21 + +--------------------------------------------------------------- + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + +NEURON { + SUFFIX Im_ms + USEION k READ ek WRITE ik + RANGE gbar, gIm, ik + RANGE modACh, maxModACh, levelACh +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.00001 (S/cm2) + modACh = 0 + maxModACh = 1 + levelACh = 0 +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + gIm (S/cm2) + mInf + mTau + mAlpha + mBeta +} + +STATE { + m +} + +BREAKPOINT { + SOLVE states METHOD cnexp + gIm = gbar*m*modulationACh() + ik = gIm*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (mInf-m)/mTau +} + +INITIAL{ + rates() + m = mInf +} + +PROCEDURE rates(){ + LOCAL qt + qt = 2.3^((34-21)/10) + + UNITSOFF + mAlpha = 3.3e-3*exp(2.5*0.04*(v - -35)) + mBeta = 3.3e-3*exp(-2.5*0.04*(v - -35)) + mInf = mAlpha/(mAlpha + mBeta) + mTau = (1/(mAlpha + mBeta))/qt + UNITSON +} + +FUNCTION modulationACh() { + : returns modulation factor + + modulationACh = 1 + modACh*(maxModACh-1)*levelACh +} diff --git a/examples/example_models/str-dspn-model2/mechanisms/bk_ms.mod b/examples/example_models/str-dspn-model2/mechanisms/bk_ms.mod new file mode 100644 index 00000000..93dd5655 --- /dev/null +++ b/examples/example_models/str-dspn-model2/mechanisms/bk_ms.mod @@ -0,0 +1,104 @@ +TITLE BK-type calcium activated K channel (KCa1.1) + +UNITS { + (molar) = (1/liter) + (mV) = (millivolt) + (mA) = (milliamp) + (mM) = (millimolar) + FARADAY = (faraday) (kilocoulombs) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX bk_ms + USEION ca READ cai + USEION k READ ek WRITE ik + RANGE gbar, ik +} + +PARAMETER { + gbar = 0.0 (mho/cm2) + k1 = 0.003 (mM) + k4 = 0.009 (mM) + d1 = 0.84 + d4 = 1.0 + q = 1 : body temperature 35 C +} + +ASSIGNED { + v (mV) + ik (mA/cm2) + celsius (degC) + cai (mM) + ek (mV) + oinf + otau (ms) +} + +STATE { o } + +BREAKPOINT { + SOLVE state METHOD cnexp + ik = gbar*o*(v-ek) +} + +DERIVATIVE state { + rate(v, cai) + o' = (oinf-o)/otau*q +} + +INITIAL { + rate(v, cai) + o = oinf +} + +PROCEDURE rate(v (mV), ca (mM)) { + LOCAL a, b, sum, z + UNITSOFF + z = 1e-3*2*FARADAY/(R*(celsius+273.15)) + a = 0.48*ca/(ca+k1*exp(-z*d1*v)) + b = 0.28/(1+ca/(k4*exp(-z*d4*v))) + sum = a+b + oinf = a/sum + otau = 1/sum + UNITSON +} + +COMMENT + +Experimental data was obtained from BKCa channels from rat brain injected +as cRNAs into Xenopus oocytes [1]. Electrophysiological recordings were +performed at room temperature 22-24 C [1, supporting online material]. + +Original model [2, model 3 in Tab.1] was implemented by De Schutter +and adapted by Kai Du [5]. In the model revisions [3,4] parameters k1 and k4 +[2, channel A in Tab.2] were adjusted to fit rat/Xenopus data +[1, Fig.3C and Fig.4A, 10 uM Ca] at body temperature 35 C. + +NEURON implementation by Alexander Kozlov . + +[1] Berkefeld H, Sailer CA, Bildl W, Rohde V, Thumfart JO, Eble S, +Klugbauer N, Reisinger E, Bischofberger J, Oliver D, Knaus HG, Schulte U, +Fakler B (2006) BKCa-Cav channel complexes mediate rapid and localized +Ca2+-activated K+ signaling. Science 314(5799):615-20. + +[2] Moczydlowski E, Latorre R (1983) Gating kinetics of Ca2+-activated K+ +channels from rat muscle incorporated into planar lipid bilayers. Evidence +for two voltage-dependent Ca2+ binding reactions. J Gen Physiol +82(4):511-42. + +[3] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH, +Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on +calcium influx and spike timing-dependent plasticity in striatal medium +spiny neurons. PLoS Comput Biol 8(4):e1002493. + +[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +[5] Du K, Wu YW, Lindroos R, Liu Y, Rózsa B, Katona G, Ding JB, +Kotaleski JH (2017) Cell-type-specific inhibition of the dendritic +plateau potential in striatal spiny projection neurons. Proc Natl Acad +Sci USA 114:E7612-E7621. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model2/mechanisms/cadyn_ms.mod b/examples/example_models/str-dspn-model2/mechanisms/cadyn_ms.mod new file mode 100644 index 00000000..472b2de9 --- /dev/null +++ b/examples/example_models/str-dspn-model2/mechanisms/cadyn_ms.mod @@ -0,0 +1,67 @@ +TITLE Calcium dynamics for N, P/Q, R calcium pool + +NEURON { + SUFFIX cadyn_ms + USEION ca READ ica, cai WRITE cai VALENCE 2 + RANGE pump, cainf, taur, drive, depth +} + +UNITS { + (molar) = (1/liter) + (mM) = (millimolar) + (um) = (micron) + (mA) = (milliamp) + (msM) = (ms mM) + FARADAY = (faraday) (coulomb) +} + +PARAMETER { + drive = 10000 (1) + depth = 0.2 (um) + cainf = 70e-6 (mM) + taur = 43 (ms) + kt = 1e-4 (mM/ms) + kd = 1e-4 (mM) + pump = 0.02 +} + +STATE { cai (mM) } + +INITIAL { cai = cainf } + +ASSIGNED { + ica (mA/cm2) + drive_channel (mM/ms) + drive_pump (mM/ms) +} + +BREAKPOINT { + SOLVE state METHOD cnexp +} + +DERIVATIVE state { + + : force concentration to stay above cainf by only pumping if larger + drive_channel = -drive*ica/(2*FARADAY*depth) + drive_pump = -kt*(cai-cainf)/(cai+kd) + + if (drive_channel <= 0.) { drive_channel = 0. } + + cai' = drive_channel + pump*drive_pump + (cainf-cai)/taur +} + +COMMENT + +Original NEURON model by Wolf (2005) and Destexhe (1992). Adaptation by +Alexander Kozlov . Updated by Robert Lindroos . + +Updates by RL: +-cainf changed from 10 to 70 nM (sabatini et al., 2002 The Life Cycle of Ca 2+ Ions in Dendritic Spines) +-pump updated to only be active if cai > cainf + +[1] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model2/mechanisms/cal12_ms.mod b/examples/example_models/str-dspn-model2/mechanisms/cal12_ms.mod new file mode 100644 index 00000000..31e29e8d --- /dev/null +++ b/examples/example_models/str-dspn-model2/mechanisms/cal12_ms.mod @@ -0,0 +1,176 @@ +TITLE HVA L-type calcium current (Cav1.2) + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (S) = (siemens) + (molar) = (1/liter) + (mM) = (millimolar) + FARADAY = (faraday) (coulomb) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX cal12_ms + USEION cal READ cali, calo WRITE ical VALENCE 2 + RANGE pbar, ical + RANGE modDA, maxModDA, levelDA + RANGE modACh, maxModACh, levelACh +} + +PARAMETER { + pbar = 0.0 (cm/s) + a = 0.17 + :q = 1 : room temperature 22-25 C + q = 2 : body temperature 35 C + modDA = 0 + maxModDA = 1 + levelDA = 0 + modACh = 0 + maxModACh = 1 + levelACh = 0 + +} + +ASSIGNED { + v (mV) + ical (mA/cm2) + ecal (mV) + celsius (degC) + cali (mM) + calo (mM) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + ical = pbar*m*(h*a+1-a)*ghk(v, cali, calo)*modulationDA()*modulationACh() +} + +INITIAL { + rates() + m = minf + h = hinf +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-8.9))/(-6.7))) + mtau = 0.06+1/(exp((v-10)/20)+exp((v-(-17))/-48)) + hinf = 1/(1+exp((v-(-13.4))/11.9)) + htau = 44.3 + UNITSON +} + +FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) { + LOCAL z, eci, eco + z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15)) + eco = co*efun(z) + eci = ci*efun(-z) + ghk = (1e-3)*2*FARADAY*(eci-eco) +} + +FUNCTION efun(z) { + if (fabs(z) < 1e-4) { + efun = 1-z/2 + }else{ + efun = z/(exp(z)-1) + } +} + + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + +FUNCTION modulationACh() { + : returns modulation factor + + modulationACh = 1 + modACh*(maxModACh-1)*levelACh +} + + +COMMENT + +Activation curve was reconstructed for cultured NAc neurons from P5-P32 +Charles River rat pups [1]. Activation time constant is from the +rodent neuron culture (both rat and mouse cells), room temperature 22-25 +C [2, Fig.15A]. Inactivation curve of CaL v1.3 current was taken from HEK +cells [3, Fig.2 and p.819] at room temperature. + +Original NEURON model by Wolf (2005) [4] was modified by Alexander Kozlov +. Kinetics of m1h type was used [5,6]. Activation +time constant was refitted to avoid singularity. + +[1] Churchill D, Macvicar BA (1998) Biophysical and pharmacological +characterization of voltage-dependent Ca2+ channels in neurons isolated +from rat nucleus accumbens. J Neurophysiol 79(2):635-47. + +[2] Kasai H, Neher E (1992) Dihydropyridine-sensitive and +omega-conotoxin-sensitive calcium channels in a mammalian +neuroblastoma-glioma cell line. J Physiol 448:161-88. + +[3] Bell DC, Butcher AJ, Berrow NS, Page KM, Brust PF, Nesterova A, +Stauderman KA, Seabrook GR, Nurnberg B, Dolphin AC (2001) Biophysical +properties, pharmacology, and modulation of human, neuronal L-type +(alpha(1D), Ca(V)1.3) voltage-dependent calcium currents. J Neurophysiol +85:816-827. + +[4] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[5] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH, +Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on +calcium influx and spike timing-dependent plasticity in striatal medium +spiny neurons. PLoS Comput Biol 8(4):e1002493. + +[6] Tuckwell HC (2012) Quantitative aspects of L-type Ca2+ currents. Prog +Neurobiol 96(1):1-31. + +add justification for modulation + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model2/mechanisms/cal13_ms.mod b/examples/example_models/str-dspn-model2/mechanisms/cal13_ms.mod new file mode 100644 index 00000000..cf4da3a3 --- /dev/null +++ b/examples/example_models/str-dspn-model2/mechanisms/cal13_ms.mod @@ -0,0 +1,178 @@ +TITLE LVA L-type calcium current (Cav1.3) + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (S) = (siemens) + (molar) = (1/liter) + (mM) = (millimolar) + FARADAY = (faraday) (coulomb) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX cal13_ms + USEION cal READ cali, calo WRITE ical VALENCE 2 + RANGE pbar, ical + RANGE modDA, maxModDA, levelDA + RANGE modACh, maxModACh, levelACh +} + +PARAMETER { + pbar = 0.0 (cm/s) + :q = 1 : room temperature 22-25 C + q = 2 : body temperature 35 C + modDA = 0 + maxModDA = 1 + levelDA = 0 + modACh = 0 + maxModACh = 1 + levelACh = 0 +} + +ASSIGNED { + v (mV) + ical (mA/cm2) + ecal (mV) + celsius (degC) + cali (mM) + calo (mM) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + ical = pbar*m*m*h*ghk(v, cali, calo)*modulationDA()*modulationACh() +} + +INITIAL { + rates() + m = minf + h = hinf +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-33))/(-6.7))) + mtau = 0.06+1/(exp((v-10)/20)+exp((v-(-17))/-48)) + hinf = 1/(1+exp((v-(-13.4))/11.9)) + htau = 44.3 + UNITSON +} + +FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) { + LOCAL z, eci, eco + z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15)) + eco = co*efun(z) + eci = ci*efun(-z) + ghk = (1e-3)*2*FARADAY*(eci-eco) +} + +FUNCTION efun(z) { + if (fabs(z) < 1e-4) { + efun = 1-z/2 + }else{ + efun = z/(exp(z)-1) + } +} + + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + +FUNCTION modulationACh() { + : returns modulation factor + + modulationACh = 1 + modACh*(maxModACh-1)*levelACh +} + + +COMMENT + +Activation curve was reconstructed for cultured NAc neurons from +P5-P32 Charles River rat pups [1] and shifted to match LVA data [7, +Fig.1D]. Activation time constant is from the rodent neuron culture (both +rat and mouse cells), room temperature 22-25 C [2, Fig.15A]. Inactivation +curve of CaL v1.3 current was taken from HEK cells [3, Fig.2 and p.819] +at room temperature. + +Original NEURON model by Wolf (2005) [4] was modified by Alexander Kozlov +. Kinetics of m2h type was used [5,6]. Activation +time constant was refitted to avoid singularity. + +[1] Churchill D, Macvicar BA (1998) Biophysical and pharmacological +characterization of voltage-dependent Ca2+ channels in neurons isolated +from rat nucleus accumbens. J Neurophysiol 79(2):635-47. + +[2] Kasai H, Neher E (1992) Dihydropyridine-sensitive and +omega-conotoxin-sensitive calcium channels in a mammalian +neuroblastoma-glioma cell line. J Physiol 448:161-88. + +[3] Bell DC, Butcher AJ, Berrow NS, Page KM, Brust PF, Nesterova A, +Stauderman KA, Seabrook GR, Nurnberg B, Dolphin AC (2001) Biophysical +properties, pharmacology, and modulation of human, neuronal L-type +(alpha(1D), Ca(V)1.3) voltage-dependent calcium currents. J Neurophysiol +85:816-827. + +[4] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[5] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH, +Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on +calcium influx and spike timing-dependent plasticity in striatal medium +spiny neurons. PLoS Comput Biol 8(4):e1002493. + +[6] Tuckwell HC (2012) Quantitative aspects of L-type Ca2+ currents. Prog +Neurobiol 96(1):1-31. + +[7] Xu W, Lipscombe D (2001) Neuronal cav1.3 L-type channels activate +at relatively hyperpolarized membrane potentials and are incompletely +inhibited by dihydropyridines. J Neurosci 21(16): 5944-5951. + + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model2/mechanisms/caldyn_ms.mod b/examples/example_models/str-dspn-model2/mechanisms/caldyn_ms.mod new file mode 100644 index 00000000..ac6484f4 --- /dev/null +++ b/examples/example_models/str-dspn-model2/mechanisms/caldyn_ms.mod @@ -0,0 +1,68 @@ +TITLE Calcium dynamics for L and T calcium pool + +NEURON { + SUFFIX caldyn_ms + USEION cal READ ical, cali WRITE cali VALENCE 2 + RANGE pump, cainf, taur, drive, depth +} + +UNITS { + (molar) = (1/liter) + (mM) = (millimolar) + (um) = (micron) + (mA) = (milliamp) + (msM) = (ms mM) + FARADAY = (faraday) (coulomb) +} + +PARAMETER { + drive = 10000 (1) + depth = 0.2 (um) + cainf = 70e-6 (mM) + taur = 43 (ms) + kt = 1e-4 (mM/ms) + kd = 1e-4 (mM) + pump = 0.02 +} + +STATE { cali (mM) } + +INITIAL { cali = cainf } + +ASSIGNED { + ical (mA/cm2) + drive_channel (mM/ms) + drive_pump (mM/ms) +} + +BREAKPOINT { + SOLVE state METHOD cnexp +} + + +DERIVATIVE state { + + : force concentration to stay above cainf by only pumping if larger + drive_channel = -drive*ical/(2*FARADAY*depth) + drive_pump = -kt*(cali-cainf)/(cali+kd) + + if (drive_channel <= 0.) { drive_channel = 0. } + + cali' = drive_channel + pump*drive_pump + (cainf-cali)/taur +} + +COMMENT + +Original NEURON model by Wolf (2005) and Destexhe (1992). Adaptation by +Alexander Kozlov . Updated by Robert Lindroos . + +Updates by RL: +-cainf changed from 10 to 70 nM (sabatini et al., 2002 The Life Cycle of Ca 2+ Ions in Dendritic Spines) +-pump updated to only be active if cai > cainf (neutralized by adding reversed entity) + +[1] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model2/mechanisms/can_ms.mod b/examples/example_models/str-dspn-model2/mechanisms/can_ms.mod new file mode 100644 index 00000000..8243d1c0 --- /dev/null +++ b/examples/example_models/str-dspn-model2/mechanisms/can_ms.mod @@ -0,0 +1,170 @@ +TITLE N-type calcium current (Cav2.2) + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (S) = (siemens) + (molar) = (1/liter) + (mM) = (millimolar) + FARADAY = (faraday) (coulomb) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX can_ms + USEION ca READ cai, cao WRITE ica VALENCE 2 + RANGE pbar, ica + RANGE modDA, maxModDA, levelDA, modACh, maxModACh, levelACh +} + +PARAMETER { + pbar = 0.0 (cm/s) + a = 0.21 + :q = 1 : room temperature 22-25 C + q = 3 : body temperature 35 C + modDA = 0 + maxModDA = 1 + levelDA = 0 + modACh = 0 + maxModACh = 1 + levelACh = 0 +} + +ASSIGNED { + v (mV) + ica (mA/cm2) + eca (mV) + celsius (degC) + cai (mM) + cao (mM) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + ica = pbar*m*m*(h*a+1-a)*ghk(v, cai, cao)*modulationDA()*modulationACh() +} + +INITIAL { + rates() + m = minf + h = hinf +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-3))/(-8))) + mtau = 0.06+1/(exp((v-25)/18)+exp((v-(-31))/(-44))) + hinf = 1/(1+exp((v-(-74.8))/6.5)) + htau = 70 + UNITSON +} + +FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) { + LOCAL z, eci, eco + z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15)) + eco = co*efun(z) + eci = ci*efun(-z) + ghk = (1e-3)*2*FARADAY*(eci-eco) +} + +FUNCTION efun(z) { + if (fabs(z) < 1e-4) { + efun = 1-z/2 + }else{ + efun = z/(exp(z)-1) + } +} + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + +FUNCTION modulationACh() { + : returns modulation factor + + modulationACh = 1 + modACh*(maxModACh-1)*levelACh +} + + +COMMENT + +Model is based on mixed data. Activation curve is from neostriatal +medium spiny neurons of adult P28+ rats [1, Fig.12F], unspecified +recording temperature. Potentials were not corrected for the liquid +junction potential, which was estimated to be 7 mV. Activation time +constant is from the rodent neuron culture (both rat and mouse cells), +room temperature 22-25 C [2, Fig.15B]. Inactivation data is from human +(HEK) cells [3, Tab.1, Tab.2], supposedly at room temperature. + +Kinetics of m2h type is used [2, Fig.5]. Activation of m^2 is fitted +to the experimental data [1,4], activation time constant corresponds +to m^2 already [2]. Original model [5,4] was modified by Alexander +Kozlov . Activation time constant was refitted to avoid +singularity in the expression. Temperature correction factor 3 is used +for body temperature [4,5]. + +[1] Bargas J, Howe A, Eberwine J, Cao Y, Surmeier DJ (1994) Cellular +and molecular characterization of Ca2+ currents in acutely isolated, +adult rat neostriatal neurons. J Neurosci 14(11 Pt 1):6667-86. + +[2] Kasai H, Neher E (1992) Dihydropyridine-sensitive and +omega-conotoxin-sensitive calcium channels in a mammalian +neuroblastoma-glioma cell line. J Physiol 448:161-88. + +[3] McNaughton NC, Randall AD (1997) Electrophysiological properties of +the human N-type Ca2+ channel: I. Channel gating in Ca2+, Ba2+ and Sr2+ +containing solutions. Neuropharmacology 36(7):895-915. + +[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +[5] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model2/mechanisms/caq_ms.mod b/examples/example_models/str-dspn-model2/mechanisms/caq_ms.mod new file mode 100644 index 00000000..357a1dc8 --- /dev/null +++ b/examples/example_models/str-dspn-model2/mechanisms/caq_ms.mod @@ -0,0 +1,105 @@ +TITLE Q-type calcium current (Cav2.1) + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (S) = (siemens) + (molar) = (1/liter) + (mM) = (millimolar) + FARADAY = (faraday) (coulomb) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX caq_ms + USEION ca READ cai, cao WRITE ica VALENCE 2 + RANGE pbar, ica +} + +PARAMETER { + pbar = 0.0 (cm/s) + :q = 1 : room temperature 22 C + q = 3 : body temperature 35 C +} + +ASSIGNED { + v (mV) + ica (mA/cm2) + eca (mV) + celsius (degC) + cai (mM) + cao (mM) + minf + mtau (ms) +} + +STATE { m } + +BREAKPOINT { + SOLVE states METHOD cnexp + ica = pbar*m*m*ghk(v, cai, cao) +} + +INITIAL { + rates() + m = minf +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-16.3))/(-7.9))) + mtau = 1.13*2 + UNITSON +} + +FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) { + LOCAL z, eci, eco + z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15)) + eco = co*efun(z) + eci = ci*efun(-z) + ghk = (1e-3)*2*FARADAY*(eci-eco) +} + +FUNCTION efun(z) { + if (fabs(z) < 1e-4) { + efun = 1-z/2 + }else{ + efun = z/(exp(z)-1) + } +} + +COMMENT + +Activation curve was reconstructed for cultured NAc neurons from P5-P32 +Charles River rat pups [1]. Activation time constant was measured in +culture neurons from cerebellum of P2-P5 rat pups [2] at room temperature +22 C. + +Original NEURON model by Wolf (2005) [3] was modified by Alexander Kozlov +. Activation curve was fitted to m2 kinetics [4], +activation time constant was scaled up as well. + +[1] Churchill D, Macvicar BA (1998) Biophysical and pharmacological +characterization of voltage-dependent Ca2+ channels in neurons isolated +from rat nucleus accumbens. J Neurophysiol 79(2):635-47. + +[2] Randall A, Tsien RW (1995) Pharmacological dissection of multiple +types of Ca2+ channel currents in rat cerebellar granule neurons. J +Neurosci 15(4):2995-3012. + +[3] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[4] Brown AM, Schwindt PC, Crill WE (1993) Voltage dependence and +activation kinetics of pharmacologically defined components of the +high-threshold calcium current in rat neocortical neurons. J Neurophysiol +70(4):1530-43. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model2/mechanisms/car_ms.mod b/examples/example_models/str-dspn-model2/mechanisms/car_ms.mod new file mode 100644 index 00000000..6a4b1413 --- /dev/null +++ b/examples/example_models/str-dspn-model2/mechanisms/car_ms.mod @@ -0,0 +1,154 @@ +TITLE R-type calcium current (Cav2.3) + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (S) = (siemens) + (molar) = (1/liter) + (mM) = (millimolar) + FARADAY = (faraday) (coulomb) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX car_ms + USEION ca READ cai, cao WRITE ica VALENCE 2 + RANGE pbar, ica + RANGE modDA, maxModDA, levelDA +} + +PARAMETER { + pbar = 0.0 (cm/s) + :q = 1 : room temperature 22 C + q = 3 : body temperature 35 C + modDA = 0 + maxModDA = 1 + levelDA = 0 +} + +ASSIGNED { + v (mV) + ica (mA/cm2) + eca (mV) + celsius (degC) + cai (mM) + cao (mM) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + ica = pbar*m*m*m*h*ghk(v, cai, cao)*modulationDA() +} + +INITIAL { + rates() + m = minf + h = hinf +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-29))/(-9.6))) + mtau = 5.1 + hinf = 1/(1+exp((v-(-33.3))/17)) + htau = 22+80/(1+exp((v-(-19))/5)) + UNITSON +} + +FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) { + LOCAL z, eci, eco + z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15)) + eco = co*efun(z) + eci = ci*efun(-z) + ghk = (1e-3)*2*FARADAY*(eci-eco) +} + +FUNCTION efun(z) { + if (fabs(z) < 1e-4) { + efun = 1-z/2 + }else{ + efun = z/(exp(z)-1) + } +} + + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + + +COMMENT + +Original data by Foehring et al (2000) [1] for dissociated MSNs from +P28-P42 Sprague-Dawley rat brain. Unspecified recording temperature. The +liquid junction potential was around 8 mV and was not corrected. Kinetics +of m3h type was fitted. Inactivation time constants were measured in +neurons from endopiriform nucleus of P7-P21 Hartley guinea pigs [2] +at room temperature 22 C. + +Original NEURON model by Wolf (2005) [3] modified by Alexander Kozlov +. Activation curve fitted to m^3 kinetics as in [4], +activation time constant matched m^3 originally [1]. Smooth fit of +inactivation time constant from [2,3]. + +[1] Foehring RC, Mermelstein PG, Song WJ, Ulrich S, Surmeier DJ +(2000) Unique properties of R-type calcium currents in neocortical and +neostriatal neurons. J Neurophysiol 84(5):2225-36. + +[2] Brevi S, de Curtis M, Magistretti J (2001) Pharmacological and +biophysical characterization of voltage-gated calcium currents in the +endopiriform nucleus of the guinea pig. J Neurophysiol 85(5):2076-87. + +[3] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model2/mechanisms/cat32_ms.mod b/examples/example_models/str-dspn-model2/mechanisms/cat32_ms.mod new file mode 100644 index 00000000..b76f310a --- /dev/null +++ b/examples/example_models/str-dspn-model2/mechanisms/cat32_ms.mod @@ -0,0 +1,103 @@ +TITLE T-type calcium current (Cav3.2) + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (S) = (siemens) + (molar) = (1/liter) + (mM) = (millimolar) + FARADAY = (faraday) (coulomb) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX cat32_ms + USEION cal READ cali, calo WRITE ical VALENCE 2 + RANGE pbar, ical +} + +PARAMETER { + pbar = 0.0 (cm/s) + :q = 1 : room temperature 21 C + q = 3 : body temperature 35 C +} + +ASSIGNED { + v (mV) + ical (mA/cm2) + ecal (mV) + celsius (degC) + cali (mM) + calo (mM) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + ical = pbar*m*m*m*h*ghk(v, cali, calo) +} + +INITIAL { + rates() + m = minf + h = hinf +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-54))/(-7.8))) + mtau = (2.1+23/(1+exp((v-(-61))/6)))*3 + hinf = 1/(1+exp((v-(-64.2))/8.8)) + htau = 30+280/(1+exp((v-(-52))/7)) + UNITSON +} + +FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) { + LOCAL z, eci, eco + z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15)) + if(z == 0) { + z = z+1e-6 + } + eco = co*(z)/(exp(z)-1) + eci = ci*(-z)/(exp(-z)-1) + ghk = (1e-3)*2*FARADAY*(eci-eco) +} + +COMMENT + +Rat Cav3.2 channels were isolated and transfection of human embryonic +kidney cells was performed [1]. Electrophysiological recordings were +done in 21 C. + +NEURON model by Alexander Kozlov . Kinetics of m3h +type was used [2-4]. Activation time constant was scaled up accordingly. + +[1] Iftinca M, McKay BE, Snutch TP, McRory JE, Turner RW, Zamponi +GW (2006) Temperature dependence of T-type calcium channel +gating. Neuroscience 142(4):1031-42. + +[2] Crunelli V, Toth TI, Cope DW, Blethyn K, Hughes SW (2005) The +'window' T-type calcium current in brain dynamics of different behavioural +states. J Physiol 562(Pt 1):121-9. + +[3] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model2/mechanisms/cat33_ms.mod b/examples/example_models/str-dspn-model2/mechanisms/cat33_ms.mod new file mode 100644 index 00000000..7c2529d5 --- /dev/null +++ b/examples/example_models/str-dspn-model2/mechanisms/cat33_ms.mod @@ -0,0 +1,103 @@ +TITLE T-type calcium current (Cav3.3) + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (S) = (siemens) + (molar) = (1/liter) + (mM) = (millimolar) + FARADAY = (faraday) (coulomb) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX cat33_ms + USEION cal READ cali, calo WRITE ical VALENCE 2 + RANGE pbar, ical +} + +PARAMETER { + pbar = 0.0 (cm/s) + :q = 1 : room temperature 21 C + q = 3 : body temperature 35 C +} + +ASSIGNED { + v (mV) + ical (mA/cm2) + ecal (mV) + celsius (degC) + cali (mM) + calo (mM) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + ical = pbar*m*m*m*h*ghk(v, cali, calo) +} + +INITIAL { + rates() + m = minf + h = hinf +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-81))/(-5.8))) + mtau = (2.3+20/(1+exp((v-(-60))/9)))*3 + hinf = 1/(1+exp((v-(-78.3))/6.5)) + htau = 125+140/(1+exp((v-(-60))/3)) + UNITSON +} + +FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) { + LOCAL z, eci, eco + z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15)) + if(z == 0) { + z = z+1e-6 + } + eco = co*(z)/(exp(z)-1) + eci = ci*(-z)/(exp(-z)-1) + ghk = (1e-3)*2*FARADAY*(eci-eco) +} + +COMMENT + +Rat Cav3.2 channels were isolated and transfection of human embryonic +kidney cells was performed [1]. Electrophysiological recordings were +done in 21 C. + +NEURON model by Alexander Kozlov . Kinetics of m3h +type was used [2-4]. Activation time constant was scaled up accordingly. + +[1] Iftinca M, McKay BE, Snutch TP, McRory JE, Turner RW, Zamponi +GW (2006) Temperature dependence of T-type calcium channel +gating. Neuroscience 142(4):1031-42. + +[2] Crunelli V, Toth TI, Cope DW, Blethyn K, Hughes SW (2005) The +'window' T-type calcium current in brain dynamics of different behavioural +states. J Physiol 562(Pt 1):121-9. + +[3] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model2/mechanisms/kaf_ms.mod b/examples/example_models/str-dspn-model2/mechanisms/kaf_ms.mod new file mode 100644 index 00000000..71b01ea7 --- /dev/null +++ b/examples/example_models/str-dspn-model2/mechanisms/kaf_ms.mod @@ -0,0 +1,168 @@ +TITLE Fast A-type potassium current (Kv4.2) + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + + +NEURON { + SUFFIX kaf_ms + USEION k READ ek WRITE ik + RANGE gbar, gk, ik, q + RANGE modDA, maxModDA, levelDA + RANGE modACh, maxModACh, levelACh + RANGE modShift +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.0 (S/cm2) + q = 1 : room temperature (unspecified) + :q = 2 : body temperature 35 C (Du 2017) + :q = 3 : body temperature 35 C + modDA = 0 + maxModDA = 1 + levelDA = 0 + modShift = 0 + modACh = 0 + maxModACh = 1 + levelACh = 0 + + + +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + gk (S/cm2) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + gk = gbar*m*m*h*modulationDA() + modShift = modulationACh() + ik = gk*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +INITIAL { + rates() + m = minf + h = hinf +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-10+modShift))/(-17.7))) + mtau = 0.9+1.1/(1+exp((v-(-30))/10)) + hinf = 1/(1+exp((v-(-75.6))/11.8)) + htau = 14 + UNITSON + + :Du 2017 + :LOCAL alpha, beta, sum + :UNITSOFF + :alpha = 1.5/(1+exp((v-4)/(-17))) + :beta = 0.6/(1+exp((v-10)/9)) + :sum = alpha+beta + :minf = alpha/sum + :mtau = 1/sum + : + :alpha = 0.105/(1+exp((v-(-121))/22)) + :beta = 0.065/(1+exp((v-(-55))/(-11))) + :sum = alpha+beta + :hinf = alpha/sum + :htau = 1/sum + :UNITSON +} + + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + +FUNCTION modulationACh() { + : returns modulation factor + + modulationACh = 1 + modACh*(maxModACh-1)*levelACh +} + +COMMENT + +Original data by Tkatch et al (2000) [1]. Neostriatal neurons were acutely +dissociated from young adult rats, age P28-P42. Electrophysiological +recordings were done at unspecified temperature (room temperature 20-22 C +assumed). Potentials were not corrected for the liquid junction potential +(estimated 1-2 mV). + +Activation m^1 matches experimental data [1, Fig.2C]. Activation time +constants fit tabulated data [1, Fig.2B]. Slope of inactivation function +fitted to the data [1, Fig.3B] with half inactivation potential -75.6 +mV. Temperature factor q between 1.5 [3] and 3 [2] was used for body +temperature. Conductance kinetics of m2h type is used [2], no corrections +for m^2 applied. Later modification by Du [4] is close to this model. + +[1] Tkatch T, Baranauskas G, Surmeier DJ (2000) Kv4.2 mRNA abundance and +A-type K(+) current amplitude are linearly related in basal ganglia and +basal forebrain neurons. J Neurosci 20(2):579-88. + +[2] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[3] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH, +Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on +calcium influx and spike timing-dependent plasticity in striatal medium +spiny neurons. PLoS Comput Biol 8(4):e1002493. + +[4] Du K, Wu YW, Lindroos R, Liu Y, Rózsa B, Katona G, Ding JB, +Kotaleski JH (2017) Cell-type-specific inhibition of the dendritic +plateau potential in striatal spiny projection neurons. Proc Natl Acad +Sci USA 114:E7612-E7621. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model2/mechanisms/kas_ms.mod b/examples/example_models/str-dspn-model2/mechanisms/kas_ms.mod new file mode 100644 index 00000000..8b01bcee --- /dev/null +++ b/examples/example_models/str-dspn-model2/mechanisms/kas_ms.mod @@ -0,0 +1,151 @@ +TITLE Slowly inactivating A-type potassium current (Kv1.2) + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + + +NEURON { + SUFFIX kas_ms + USEION k READ ek WRITE ik + RANGE gbar, gk, ik + RANGE modDA, maxModDA, levelDA + +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.0 (S/cm2) + a = 0.8 + :q = 1 : room temperature 22-24 C + q = 3 : body temperature 33 C + modDA = 0 + maxModDA = 1 + levelDA = 0 + +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + gk (S/cm2) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + gk = gbar*m*m*(h*a+1-a)*modulationDA() + ik = gk*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +INITIAL { + rates() + m = minf + h = hinf +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-27))/(-16))) + mtau = 3.4+89.2*exp(-((v-(-34.3))/30.1)^2) + hinf = 1/(1+exp((v-(-33.5))/21.5)) + htau = 548.7*6/(exp((v-(-96))/(-29.01))+exp((v-(-96))/100)) + : Du 2017 + :LOCAL alpha, beta, sum + :UNITSOFF + :alpha = 0.25/(1+exp((v-50)/(-20))) + :beta = 0.05/(1+exp((v-(-90))/35)) + :sum = alpha+beta + :minf = alpha/sum + :mtau = 1/sum + : + :alpha = 0.0025/(1+exp((v-(-95))/16)) + :beta = 0.002/(1+exp((v-50)/(-70))) + :sum = alpha+beta + :hinf = a+(alpha/sum)*(1-a) + :htau = 1/sum + UNITSON +} + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + + +COMMENT + +Experimental data by Shen et al (2004) [1]. Medium spiny neurons were +acutely dissociated from from young adult (P21-P28) Sprague-Dawley rat +brain. All recordings were conducted at 22-24 C. No correction for the +liquid junction potential was reported. + +Conductance kinetics of m2h type is used [1,2] with partial inactivation, +m2 (a h + (1-a)). Fraction a is set to 0.8, as in [1, Fig.6B]; other +values for a are possible [2] (see also kas.mod in companion code). +Equation for htau [1] is corrected to match the authors' data [1, Fig.6B]. +Time constants were corrected to body temperature with factor q=3 [1-3]. +Later modification by Du [4] is close to this model with adjusted +inactivation. + +[1] Shen W, Hernandez-Lopez S, Tkatch T, Held JE, Surmeier DJ (2004) +Kv1.2-containing K+ channels regulate subthreshold excitability of +striatal medium spiny neurons. J Neurophysiol 91(3):1337-49. + +[2] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[3] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +[4] Du K, Wu YW, Lindroos R, Liu Y, Rózsa B, Katona G, Ding JB, +Kotaleski JH (2017) Cell-type-specific inhibition of the dendritic +plateau potential in striatal spiny projection neurons. Proc Natl Acad +Sci USA 114:E7612-E7621. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model2/mechanisms/kdr_ms.mod b/examples/example_models/str-dspn-model2/mechanisms/kdr_ms.mod new file mode 100644 index 00000000..1a147689 --- /dev/null +++ b/examples/example_models/str-dspn-model2/mechanisms/kdr_ms.mod @@ -0,0 +1,79 @@ +TITLE Fast delayed rectifier potassium current (Kv3.1/3.2) + +NEURON { + SUFFIX kdr_ms + USEION k READ ek WRITE ik + RANGE gbar, gk, ik +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.0 (S/cm2) + q = 1 +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + gk (S/cm2) + minf + mtau (ms) +} + +STATE { m } + +BREAKPOINT { + SOLVE states METHOD cnexp + gk = gbar*m + ik = gk*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q +} + +INITIAL { + rates() + m = minf +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-13))/(-6))) + mtau = 11.1 + UNITSON +} + +COMMENT + +Original data and model by Baranauskas et al (1999) [1] for globus +pallidus neurons from young adult rat. The temperature was not +specified. Potentials were not corrected for the liquid junction +potential, which was estimated to be 1-2 mV. + +Kinetics of m^1 type is used as in [2,3]. Room temperature 20-23 C +is assumed. + +NEURON implementation by Alexander Kozlov . + +[1] Baranauskas G, Tkatch T, Surmeier DJ (1999) Delayed rectifier currents +in rat globus pallidus neurons are attributable to Kv2.1 and Kv3.1/3.2 +K(+) channels. J Neurosci 19(15):6394-404. + +[2] Migliore M, Hoffman DA, Magee JC, Johnston D (1999) Role of an +A-type K+ conductance in the back-propagation of action potentials in the +dendrites of hippocampal pyramidal neurons. J Comput Neurosci 7(1):5-15. + +[3] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH, +Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on +calcium influx and spike timing-dependent plasticity in striatal medium +spiny neurons. PLoS Comput Biol 8(4):e1002493. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model2/mechanisms/kir_ms.mod b/examples/example_models/str-dspn-model2/mechanisms/kir_ms.mod new file mode 100644 index 00000000..5afd9e84 --- /dev/null +++ b/examples/example_models/str-dspn-model2/mechanisms/kir_ms.mod @@ -0,0 +1,147 @@ +TITLE Non-inactivating inwardly rectifying potassium current (Kir2.3) + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + + +NEURON { + SUFFIX kir_ms + USEION k READ ek WRITE ik + RANGE gbar, gk, ik, shift + RANGE modDA, maxModDA, levelDA + RANGE modACh, maxModACh, levelACh +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.0 (S/cm2) + shift = 0.0 (mV) + q = 1 : body temperature 35 C + modDA = 0 + maxModDA = 1 + levelDA = 0 + modACh = 0 + maxModACh = 1 + levelACh = 0 +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + gk (S/cm2) + minf + mtau (ms) +} + +STATE { m } + +BREAKPOINT { + SOLVE states METHOD cnexp + gk = gbar*m*modulationDA()*modulationACh() + ik = gk*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q +} + +INITIAL { + rates() + m = minf +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-82)-shift)/13)) + mtau = 1/(exp((v-(-103))/(-14.5))+0.125/(1+exp((v-(-35))/(-19)))) + UNITSON +} + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + +FUNCTION modulationACh() { + : returns modulation factor + + modulationACh = 1 + modACh*(maxModACh-1)*levelACh +} + +COMMENT + +Original model by Wolf et al (2005) [1] for the rat MSN cells from the +nucleus accumbens. The activation curve was fitted to a mouse Kir2.1 +channel expressed in HEK cells [2] and shifted to match extracellular +concentration of K in rat. Measured half-activation values are -109.3 +mV (striatonigral MSN) and -113.2 mV (striatopallidal MSN) [6, Supp +Tab.1]. Time constants were derived from Aplysia data [3] and adjusted +to match the rat experiments [1]. Time constant was further tuned [4] +to fit the rat data below -80 mV [5]. Kinetics is corrected to the body +temperature 35 C [4]. + +Non-inactivating Kir current was observed in cells expressing Kir2.2 +and/or Kir2.3 [5]. Activation variable with m^1 kinetics is used [1,4]. +Smooth fit of the time constants by Alexander Kozlov . + +[1] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[2] Kubo Y, Murata Y (2001) Control of rectification and permeation by +two distinct sites after the second transmembrane region in Kir2.1 K+ +channel. J Physiol 531, 645-660. + +[3] Hayashi H, Fishman HM (1988) Inward rectifier K+ channel kinetics +from analysis of the complex conductance of aplysia neuronal membrane. +Biophys J 53, 747-757. + +[4] Steephen JE, Manchanda R (2009) Differences in biophysical properties +of nucleus accumbens medium spiny neurons emerging from inactivation of +inward rectifying potassium currents. J Comput Neurosci 27(3):453-70 + +[5] Mermelstein PG, Song WJ, Tkatch T, Yan Z, Surmeier DJ (1998) +Inwardly rectifying potassium (IRK) currents are correlated with IRK +subunit expression in rat nucleus accumbens medium spiny neurons. J +Neurosci 18(17):6650-61. + +[6] Shen W, Tian X, Day M, Ulrich S, Tkatch T, Nathanson NM, Surmeier DJ +(2007) Cholinergic modulation of Kir2 channels selectively elevates +dendritic excitability in striatopallidal neurons. Nat Neurosci +10(11):1458-66. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model2/mechanisms/naf_ms.mod b/examples/example_models/str-dspn-model2/mechanisms/naf_ms.mod new file mode 100644 index 00000000..f676971f --- /dev/null +++ b/examples/example_models/str-dspn-model2/mechanisms/naf_ms.mod @@ -0,0 +1,133 @@ +TITLE Fast transient sodium current + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + +NEURON { + SUFFIX naf_ms + USEION na READ ena WRITE ina + RANGE gbar, gna, ina + RANGE modDA, maxModDA, levelDA + RANGE modACh, maxModACh, levelACh +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.0 (S/cm2) + :q = 1 : room temperature 22 C + q = 1.8 : body temperature 35 C + modDA = 0 + maxModDA = 1 + levelDA = 0 + modACh = 0 + maxModACh = 1 + levelACh = 0 +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + gna (S/cm2) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + gna = gbar*m*m*m*h*modulationDA()*modulationACh() + ina = gna*(v-ena) +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +INITIAL { + rates() + m = minf + h = hinf +} + +PROCEDURE rates() { + UNITSOFF + :minf = 1/(1+exp((v-(-25.5))/(-9.2))) + :mtau = 0.33+1/(exp((v-(-62))/14)+exp((v-(-60))/(-17))) + :hinf = 1/(1+exp((v-(-63.2))/6)) + :htau = 0.6+1/(exp((v-(-44))/8)+exp((v-(-99))/(-44))) + minf = 1/(1+exp((v-(-25))/(-10))) + mtau = 0.33+1/(exp((v-(-62))/14)+exp((v-(-60))/(-17))) + hinf = 1/(1+exp((v-(-62))/6)) + htau = 0.6+1/(exp((v-(-44))/8)+exp((v-(-99))/(-44))) + UNITSON +} + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + +FUNCTION modulationACh() { + : returns modulation factor + + modulationACh = 1 + modACh*(maxModACh-1)*levelACh +} + +COMMENT + +Original data by Ogata and Tatebayashi (1990) [1]. Neostriatal neurons +of medium size (putative medium spiny neurons) freshly isolated from +the adult guinea pig brain (either sex, 200 g). Data compensated for +the liquid junction potential (-13 mV). Experiments carried out at room +temperature (22 C). Conductance fitted by m3h kinetics. + +Smooth fit of mtau and htau data [1] by Alexander Kozlov +assuming natural logarithm of tau values [1, Figs. 5 and 9] and +temperature correction factor of 1.8 [2] as suggested by Robert Lindroos +. + +[1] Ogata N, Tatebayashi H (1990) Sodium current kinetics in freshly +isolated neostriatal neurones of the adult guinea pig. Pflugers Arch +416(5):594-603. + +[2] Schwarz JR (1986) The effect of temperature on Na currents in rat +myelinated nerve fibres. Pflugers Arch. 406(4):397-404. + +ENDCOMMENT diff --git a/examples/example_models/str-dspn-model2/mechanisms/sk_ms.mod b/examples/example_models/str-dspn-model2/mechanisms/sk_ms.mod new file mode 100644 index 00000000..c25fe7dc --- /dev/null +++ b/examples/example_models/str-dspn-model2/mechanisms/sk_ms.mod @@ -0,0 +1,83 @@ +TITLE SK-type calcium activated K channel (KCa2.2) + +UNITS { + (molar) = (1/liter) + (mV) = (millivolt) + (mA) = (milliamp) + (mM) = (millimolar) +} + +NEURON { + SUFFIX sk_ms + USEION ca READ cai + USEION k READ ek WRITE ik + RANGE gbar, ik +} + +PARAMETER { + gbar = 0.0 (mho/cm2) + :q = 1 : room temperature + q = 1 : body temperature +} + +ASSIGNED { + v (mV) + ik (mA/cm2) + cai (mM) + ek (mV) + oinf + otau (ms) +} + +STATE { o } + +BREAKPOINT { + SOLVE state METHOD cnexp + ik = gbar*o*(v-ek) +} + +DERIVATIVE state { + rate(v, cai) + o' = (oinf-o)/otau*q +} + +INITIAL { + rate(v, cai) + o = oinf +} + +PROCEDURE rate(v (mV), ca (mM)) { + LOCAL a + a = (ca/0.57e-3)^5.2 + oinf = a/(1+a) + otau = 4.9 +} + +COMMENT + +Experimental data was obtained for the apamin-sensitive clone rSK2 from +rat brain cDNA expressed in Xenopus oocytes [1,2]. All experiments were +performed at room tempretaure. + +Original model [3] used calcium dependence from [2, Fig.2] and calcium +activation time constant from [1, Fig.13]. NEURON implementation by +Alexander Kozlov follows the revised model [4]. + +[1] Hirschberg B, Maylie J, Adelman JP, Marrion NV (1998) Gating of +recombinant small-conductance Ca-activated K+ channels by calcium. J +Gen Physiol 111(4):565-81. + +[2] Maylie J, Bond CT, Herson PS, Lee WS, Adelman JP (2004) Small +conductance Ca2+-activated K+ channels and calmodulin. J Physiol 554(Pt +2):255-61. + +[3] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH, +Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on +calcium influx and spike timing-dependent plasticity in striatal medium +spiny neurons. PLoS Comput Biol 8(4):e1002493. + +[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +ENDCOMMENT diff --git a/examples/example_models/dspn/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var0.swc b/examples/example_models/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var0.swc similarity index 100% rename from examples/example_models/dspn/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var0.swc rename to examples/example_models/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var0.swc diff --git a/examples/example_models/dspn/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var1.swc b/examples/example_models/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var1.swc similarity index 100% rename from examples/example_models/dspn/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var1.swc rename to examples/example_models/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var1.swc diff --git a/examples/example_models/dspn/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var2.swc b/examples/example_models/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var2.swc similarity index 100% rename from examples/example_models/dspn/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var2.swc rename to examples/example_models/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var2.swc diff --git a/examples/example_models/dspn/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var3.swc b/examples/example_models/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var3.swc similarity index 100% rename from examples/example_models/dspn/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var3.swc rename to examples/example_models/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var3.swc diff --git a/examples/example_models/dspn/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var4.swc b/examples/example_models/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var4.swc similarity index 100% rename from examples/example_models/dspn/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var4.swc rename to examples/example_models/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var4.swc diff --git a/examples/example_models/dspn/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var5.swc b/examples/example_models/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var5.swc similarity index 100% rename from examples/example_models/dspn/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var5.swc rename to examples/example_models/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var5.swc diff --git a/examples/example_models/dspn/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var6.swc b/examples/example_models/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var6.swc similarity index 100% rename from examples/example_models/dspn/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var6.swc rename to examples/example_models/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var6.swc diff --git a/examples/example_models/dspn/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var7.swc b/examples/example_models/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var7.swc similarity index 100% rename from examples/example_models/dspn/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var7.swc rename to examples/example_models/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var7.swc diff --git a/examples/example_models/dspn/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var8.swc b/examples/example_models/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var8.swc similarity index 100% rename from examples/example_models/dspn/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var8.swc rename to examples/example_models/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3-var8.swc diff --git a/examples/example_models/dspn/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3.swc b/examples/example_models/str-dspn-model2/morphology/21-6-DE-cor-rep-ax-res3.swc similarity index 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"gbar_kaf_fs.somatic": 0.5168279128796623, + "gbar_kas_fs.somatic": 0.008679283146225007, + "gbar_kdr_fs.somatic": 0.0038674216223084585, + "gbar_kir_fs.basal": 6.589376097975843e-06, + "gbar_kir_fs.somatic": 0.0017300725624570705, + "gbar_naf_fs.somatic": 8.37092170025024, + "q_kaf_fs.somatic": 3.171332068240642, + "q_kdr_fs.somatic": 5.825934815538842, + "q_naf_fs.somatic": 3.5599964888028546 + }, + { + "Ra.all": 157.20677579990664, + "e_pas.all": -68.23137871676171, + "g_pas.all": 0.00010242686876100648, + "gbar_bk_fs.somatic": 2.7350994934503903e-06, + "gbar_kaf_fs.somatic": 0.5084364538481287, + "gbar_kas_fs.somatic": 0.007791510465544893, + "gbar_kdr_fs.somatic": 0.008686223839903414, + "gbar_kir_fs.basal": 4.555740995504902e-05, + "gbar_kir_fs.somatic": 0.0011674418830140084, + "gbar_naf_fs.somatic": 8.226731504838222, + "q_kaf_fs.somatic": 3.106160542549146, + "q_kdr_fs.somatic": 5.388470625572981, + "q_naf_fs.somatic": 3.746392904906477 + }, + { + "Ra.all": 144.31832337794006, + "e_pas.all": -65.27237751737432, + "g_pas.all": 0.00010480548073392352, + "gbar_bk_fs.somatic": 1.7332853827710163e-05, + "gbar_kaf_fs.somatic": 0.5168279128796623, + "gbar_kas_fs.somatic": 0.008679283146225007, + "gbar_kdr_fs.somatic": 0.00841817230715603, + "gbar_kir_fs.basal": 8.656033249833996e-05, + "gbar_kir_fs.somatic": 0.001100887069712914, + "gbar_naf_fs.somatic": 8.37092170025024, + "q_kaf_fs.somatic": 3.395250337489538, + "q_kdr_fs.somatic": 5.874686692704899, + "q_naf_fs.somatic": 3.6061349412150623 + } +] diff --git a/examples/example_models/str-fs-model1/config/features.json b/examples/example_models/str-fs-model1/config/features.json new file mode 100644 index 00000000..393afab1 --- /dev/null +++ b/examples/example_models/str-fs-model1/config/features.json @@ -0,0 +1,206 @@ +{ + "IV_483": { + "soma": { + "decay_time_constant_after_stim": [ + 1.3725, + 1.39 + ], + "steady_state_voltage_stimend": [ + -106.0399, + 1.0 + ], + "voltage_base": [ + -90.1097, + 1.0 + ], + "voltage_deflection": [ + -15.8091, + 1.0 + ] + } + }, + "IV_487": { + "soma": { + "decay_time_constant_after_stim": [ + 4.7138, + 1.39 + ], + "steady_state_voltage_stimend": [ + -97.238, + 1.0 + ], + "voltage_base": [ + -88.0029, + 1.0 + ], + "voltage_deflection": [ + -9.3958, + 1.0 + ] + } + }, + "IV_494": { + "soma": { + "decay_time_constant_after_stim": [ + 5.8919, + 3.83 + ], + "steady_state_voltage_stimend": [ + -66.9306, + 1.0 + ], + "voltage_base": [ + -88.5579, + 1.0 + ], + "voltage_deflection": [ + 21.0356, + 1.0 + ] + } + }, + "IDthresh-sub_525": { + "soma": { + "Spikecount": [ + 0.0, + 0.001 + ], + "decay_time_constant_after_stim": [ + 5.6042, + 3.83 + ], + "steady_state_voltage": [ + -87.5766, + 2.0 + ], + "steady_state_voltage_stimend": [ + -60.8071, + 1.0 + ], + "voltage_base": [ + -88.0154, + 1.0 + ], + "voltage_deflection": [ + 26.7593, + 1.0 + ], + "voltage_deflection_begin": [ + 26.4198, + 1.0 + ] + } + }, + "IDthresh_528": { + "soma": { + "AHP_depth_abs": [ + -62.49, + 0.3924 + ], + "AP_begin_voltage": [ + -44.4029, + 0.8216 + ], + "inv_first_ISI": [ + 14.9701, + 1.497 + ], + "steady_state_voltage": [ + -88.1857, + 2.0 + ], + "time_to_first_spike": [ + 104.0, + 10.4 + ], + "voltage_base": [ + -88.459, + 1.0 + ] + } + }, + "IDthresh_531": { + "soma": { + "AHP_depth_abs": [ + -61.7387, + 0.6339 + ], + "AP_begin_voltage": [ + -43.5831, + 1.1421 + ], + "inv_first_ISI": [ + 41.4938, + 4.1494 + ], + "steady_state_voltage": [ + -88.9817, + 2.0 + ], + "time_to_first_spike": [ + 68.6, + 6.86 + ], + "voltage_base": [ + -89.1793, + 8.6558 + ] + } + }, + "IDthresh_535": { + "soma": { + "AHP_depth_abs": [ + -60.7346, + 0.6577 + ], + "AP_begin_voltage": [ + -43.6314, + 5.5997 + ], + "inv_first_ISI": [ + 57.4713, + 5.7471 + ], + "steady_state_voltage": [ + -89.5213, + 2.0 + ], + "time_to_first_spike": [ + 52.7, + 5.27 + ], + "voltage_base": [ + -89.658, + 1.0 + ] + } + }, + "IDthresh_542": { + "soma": { + "AHP_depth_abs": [ + -58.6646, + 0.7986 + ], + "AP_begin_voltage": [ + -42.2627, + 4.5274 + ], + "inv_first_ISI": [ + 107.5269, + 10.7527 + ], + "steady_state_voltage": [ + -90.6569, + 2.0 + ], + "time_to_first_spike": [ + 38.6, + 3.86 + ], + "voltage_base": [ + -89.4166, + 8.6558 + ] + } + } +} diff --git a/examples/example_models/fs/str-fs-model1/mechanisms.json b/examples/example_models/str-fs-model1/config/mechanisms.json similarity index 99% rename from examples/example_models/fs/str-fs-model1/mechanisms.json rename to examples/example_models/str-fs-model1/config/mechanisms.json index 33034601..f3bde8f6 100644 --- a/examples/example_models/fs/str-fs-model1/mechanisms.json +++ b/examples/example_models/str-fs-model1/config/mechanisms.json @@ -15,4 +15,4 @@ "basal": [ "kir_fs" ] -} \ No newline at end of file +} diff --git a/examples/example_models/str-fs-model1/config/parameters.json b/examples/example_models/str-fs-model1/config/parameters.json new file mode 100644 index 00000000..1d3e8209 --- /dev/null +++ b/examples/example_models/str-fs-model1/config/parameters.json @@ -0,0 +1,160 @@ +[ + { + "param_name": "celsius", + "type": "global", + "value": 35 + }, + { + "param_name": "v_init", + "type": "global", + "value": -85 + }, + { + "param_name": "g_pas", + "sectionlist": "all", + "type": "section", + "dist_type": "uniform", + "bounds": [1e-5, 1e-3] + }, + { + "param_name": "e_pas", + "sectionlist": "all", + "type": "section", + "dist_type": "uniform", + "bounds": [-80, -60] + }, + { + "param_name": "cm", + "sectionlist": "all", + "type": "section", + "dist_type": "uniform", + "value": 1 + }, + { + "param_name": "Ra", + "sectionlist": "all", + "type": "section", + "dist_type": "uniform", + "bounds": [100, 300] + }, + { + "param_name": "ek", + "sectionlist": "somatic", + "type": "section", + "dist_type": "uniform", + "value": -105.9 + }, + { + "param_name": "ek", + "sectionlist": "basal", + "type": "section", + "dist_type": "uniform", + "value": -105.9 + }, + { + "param_name": "ena", + "sectionlist": "somatic", + "type": "section", + "dist_type": "uniform", + "value": 53.34 + }, + { + "param_name": "gbar_naf_fs", + "mech": "naf_fs", + "bounds": [0, 15] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "q_naf_fs", + "mech": "naf_fs", + "bounds": [2, 5] , + "dist_type": "uniform", + "mech_param": "q", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "gbar_kdr_fs", + "mech": "kdr_fs", + "bounds": [0, 0.1] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "q_kdr_fs", + "mech": "kdr_fs", + "bounds": [3, 6] , + "dist_type": "uniform", + "mech_param": "q", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "gbar_kaf_fs", + "mech": "kaf_fs", + "bounds": [0, 1] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "q_kaf_fs", + "mech": "kaf_fs", + "bounds": [3, 6] , + "dist_type": "uniform", + "mech_param": "q", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "gbar_kas_fs", + "mech": "kas_fs", + "bounds": [0, 0.01] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "pbar_caq_fs", + "mech": "caq_fs", + "value": 6e-6 , + "dist_type": "uniform", + "mech_param": "pbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "gbar_bk_fs", + "mech": "bk_fs", + "bounds": [0, 0.0001] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "gbar_kir_fs", + "mech": "kir_fs", + "bounds": [0, 0.01] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "somatic" + }, + { + "param_name": "gbar_kir_fs", + "mech": "kir_fs", + "bounds": [0, 0.001] , + "dist_type": "uniform", + "mech_param": "gbar", + "type": "range", + "sectionlist": "basal" + } +] diff --git a/examples/example_models/str-fs-model1/config/protocols.json b/examples/example_models/str-fs-model1/config/protocols.json new file mode 100644 index 00000000..8b40a9b8 --- /dev/null +++ b/examples/example_models/str-fs-model1/config/protocols.json @@ -0,0 +1,130 @@ +{ + "IV_483": { + "stimuli": [ + { + "delay": 100.0, + "amp": -0.3132, + "duration": 1000.0, + "totduration": 1400.0 + }, + { + "delay": 0.0, + "amp": 0.0081, + "duration": 1400.0, + "totduration": 1400.0 + } + ] + }, + "IV_487": { + "stimuli": [ + { + "delay": 100.0, + "amp": -0.1343, + "duration": 1000.0, + "totduration": 1400.0 + }, + { + "delay": 0.0, + "amp": 0.0081, + "duration": 1400.0, + "totduration": 1400.0 + } + ] + }, + "IV_494": { + "stimuli": [ + { + "delay": 100.0, + "amp": 0.179, + "duration": 1000.0, + "totduration": 1400.0 + }, + { + "delay": 0.0, + "amp": 0.0081, + "duration": 1400.0, + "totduration": 1400.0 + } + ] + }, + "IDthresh-sub_525": { + "stimuli": [ + { + "delay": 700.0, + "amp": 0.2215, + "duration": 2000.0, + "totduration": 3000.0 + }, + { + "delay": 0.0, + "amp": 0.0081, + "duration": 3000.0, + "totduration": 3000.0 + } + ] + }, + "IDthresh_528": { + "stimuli": [ + { + "delay": 700.0, + "amp": 0.2418, + "duration": 2000.0, + "totduration": 3000.0 + }, + { + "delay": 0.0, + "amp": 0.0081, + "duration": 3000.0, + "totduration": 3000.0 + } + ] + }, + "IDthresh_531": { + "stimuli": [ + { + "delay": 700.0, + "amp": 0.2618, + "duration": 2000.0, + "totduration": 3000.0 + }, + { + "delay": 0.0, + "amp": 0.0081, + "duration": 3000.0, + "totduration": 3000.0 + } + ] + }, + "IDthresh_535": { + "stimuli": [ + { + "delay": 700.0, + "amp": 0.2887, + "duration": 2000.0, + "totduration": 3000.0 + }, + { + "delay": 0.0, + "amp": 0.0081, + "duration": 3000.0, + "totduration": 3000.0 + } + ] + }, + "IDthresh_542": { + "stimuli": [ + { + "delay": 700.0, + "amp": 0.3355, + "duration": 2000.0, + "totduration": 3000.0 + }, + { + "delay": 0.0, + "amp": 0.0081, + "duration": 3000.0, + "totduration": 3000.0 + } + ] + } +} diff --git a/examples/example_models/str-fs-model1/hall_of_fame.json b/examples/example_models/str-fs-model1/hall_of_fame.json new file mode 100644 index 00000000..07da31f7 --- /dev/null +++ b/examples/example_models/str-fs-model1/hall_of_fame.json @@ -0,0 +1,107 @@ +[ + { + "Ra.all": 145.4895183121606, + "e_pas.all": -65.35030542297895, + "g_pas.all": 0.00010238690542408328, + "gbar_bk_fs.somatic": 5.814610645872279e-06, + "gbar_kaf_fs.somatic": 0.4424476257758355, + "gbar_kas_fs.somatic": 0.009961028480984431, + "gbar_kdr_fs.somatic": 0.002692972004578943, + "gbar_kir_fs.basal": 0.00011188186722063707, + "gbar_kir_fs.somatic": 0.0011009535410814706, + "gbar_naf_fs.somatic": 8.37092170025024, + "q_kaf_fs.somatic": 3.171332068240642, + "q_kdr_fs.somatic": 5.994401267554457, + "q_naf_fs.somatic": 3.6073127425562506 + }, + { + "Ra.all": 194.38950512756048, + "e_pas.all": -69.32265011443972, + 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0.0008367848966099811, + "gbar_naf_fs.somatic": 8.761458381181264, + "q_kaf_fs.somatic": 3.8406865493069526, + "q_kdr_fs.somatic": 5.415593381734021, + "q_naf_fs.somatic": 3.7025935692933496 + }, + { + "Ra.all": 119.30531220990797, + "e_pas.all": -65.66188163549452, + "g_pas.all": 9.946804090676818e-05, + "gbar_bk_fs.somatic": 3.303260730569257e-05, + "gbar_kaf_fs.somatic": 0.45179228334127935, + "gbar_kas_fs.somatic": 0.0068127403897264675, + "gbar_kdr_fs.somatic": 0.006484314217429479, + "gbar_kir_fs.basal": 0.00013635875524162325, + "gbar_kir_fs.somatic": 0.0008353255992959469, + "gbar_naf_fs.somatic": 7.254805438391028, + "q_kaf_fs.somatic": 4.017958986934227, + "q_kdr_fs.somatic": 5.971058623176278, + "q_naf_fs.somatic": 2.1830452446033712 + }, + { + "Ra.all": 161.62420041966823, + "e_pas.all": -65.27237751737432, + "g_pas.all": 0.00010242686876100648, + "gbar_bk_fs.somatic": 4.758975961515347e-06, + "gbar_kaf_fs.somatic": 0.5168279128796623, + "gbar_kas_fs.somatic": 0.008679283146225007, + "gbar_kdr_fs.somatic": 0.0038674216223084585, + "gbar_kir_fs.basal": 6.589376097975843e-06, + "gbar_kir_fs.somatic": 0.0017300725624570705, + "gbar_naf_fs.somatic": 8.37092170025024, + "q_kaf_fs.somatic": 3.171332068240642, + "q_kdr_fs.somatic": 5.825934815538842, + "q_naf_fs.somatic": 3.5599964888028546 + } +] diff --git a/examples/example_models/str-fs-model1/mechanisms/bk_fs.mod b/examples/example_models/str-fs-model1/mechanisms/bk_fs.mod new file mode 100644 index 00000000..21bef2fd --- /dev/null +++ b/examples/example_models/str-fs-model1/mechanisms/bk_fs.mod @@ -0,0 +1,99 @@ +TITLE BK-type calcium activated K channel (KCa1.1) + +UNITS { + (molar) = (1/liter) + (mV) = (millivolt) + (mA) = (milliamp) + (mM) = (millimolar) + FARADAY = (faraday) (kilocoulombs) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX bk_fs + USEION ca READ cai + USEION k READ ek WRITE ik + RANGE gbar, ik +} + +PARAMETER { + gbar = 0.0 (mho/cm2) + k1 = 0.003 (mM) + k4 = 0.009 (mM) + d1 = 0.84 + d4 = 1.0 + q = 1 : body temperature 35 C +} + +ASSIGNED { + v (mV) + ik (mA/cm2) + celsius (degC) + cai (mM) + ek (mV) + oinf + otau (ms) +} + +STATE { o } + +BREAKPOINT { + SOLVE state METHOD cnexp + ik = gbar*o*(v-ek) +} + +DERIVATIVE state { + rate(v, cai) + o' = (oinf-o)/otau*q +} + +INITIAL { + rate(v, cai) + o = oinf +} + +PROCEDURE rate(v (mV), ca (mM)) { + LOCAL a, b, sum, z + UNITSOFF + z = 1e-3*2*FARADAY/(R*(celsius+273.15)) + a = 0.48*ca/(ca+k1*exp(-z*d1*v)) + b = 0.28/(1+ca/(k4*exp(-z*d4*v))) + sum = a+b + oinf = a/sum + otau = 1/sum + UNITSON +} + +COMMENT + +Experimental data was obtained from BKCa channels from rat brain injected +as cRNAs into Xenopus oocytes [1]. Electrophysiological recordings were +performed at room temperature 22-24 C [1, supporting online material]. + +Original model [2, model 3 in Tab.1] was implemented by De Schutter +and adapted by Kai Du. In the model revisions [3,4] parameters k1 and k4 +[2, channel A in Tab.2] were adjusted to fit rat/Xenopus data +[1, Fig.3C and Fig.4A, 10 uM Ca] at body temperature 35 C. + +NEURON implementation by Alexander Kozlov . + +[1] Berkefeld H, Sailer CA, Bildl W, Rohde V, Thumfart JO, Eble S, +Klugbauer N, Reisinger E, Bischofberger J, Oliver D, Knaus HG, Schulte U, +Fakler B (2006) BKCa-Cav channel complexes mediate rapid and localized +Ca2+-activated K+ signaling. Science 314(5799):615-20. + +[2] Moczydlowski E, Latorre R (1983) Gating kinetics of Ca2+-activated K+ +channels from rat muscle incorporated into planar lipid bilayers. Evidence +for two voltage-dependent Ca2+ binding reactions. J Gen Physiol +82(4):511-42. + +[3] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH, +Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on +calcium influx and spike timing-dependent plasticity in striatal medium +spiny neurons. PLoS Comput Biol 8(4):e1002493. + +[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +ENDCOMMENT diff --git a/examples/example_models/str-fs-model1/mechanisms/cadyn_fs.mod b/examples/example_models/str-fs-model1/mechanisms/cadyn_fs.mod new file mode 100644 index 00000000..17651d8a --- /dev/null +++ b/examples/example_models/str-fs-model1/mechanisms/cadyn_fs.mod @@ -0,0 +1,59 @@ +TITLE Calcium dynamics for N, P/Q, R calcium pool + +NEURON { + SUFFIX cadyn_fs + USEION ca READ ica, cai WRITE cai VALENCE 2 + RANGE pump, cainf, taur, drive +} + +UNITS { + (molar) = (1/liter) + (mM) = (millimolar) + (um) = (micron) + (mA) = (milliamp) + (msM) = (ms mM) + FARADAY = (faraday) (coulomb) +} + +PARAMETER { + drive = 10000 (1) + depth = 0.1 (um) + cainf = 10e-6 (mM) + taur = 43 (ms) + kt = 1e-4 (mM/ms) + kd = 1e-4 (mM) + pump = 0.02 +} + +STATE { cai (mM) } + +INITIAL { cai = cainf } + +ASSIGNED { + ica (mA/cm2) + drive_channel (mM/ms) + drive_pump (mM/ms) +} + +BREAKPOINT { + SOLVE state METHOD cnexp +} + +DERIVATIVE state { + drive_channel = -drive*ica/(2*FARADAY*depth) + if (drive_channel <= 0.) { drive_channel = 0. } + drive_pump = -kt*cai/(cai+kd) + cai' = (drive_channel+pump*drive_pump+(cainf-cai)/taur) +} + +COMMENT + +Original NEURON model by Wolf (2005) and Destexhe (1992). Adaptation by +Alexander Kozlov . + +[1] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +ENDCOMMENT diff --git a/examples/example_models/str-fs-model1/mechanisms/can_fs.mod b/examples/example_models/str-fs-model1/mechanisms/can_fs.mod new file mode 100644 index 00000000..0bea8c12 --- /dev/null +++ b/examples/example_models/str-fs-model1/mechanisms/can_fs.mod @@ -0,0 +1,121 @@ +TITLE N-type calcium current (Cav2.2) + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (S) = (siemens) + (molar) = (1/liter) + (mM) = (millimolar) + FARADAY = (faraday) (coulomb) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX can_fs + USEION ca READ cai, cao WRITE ica VALENCE 2 + RANGE pbar, ica +} + +PARAMETER { + pbar = 0.0 (cm/s) + a = 0.21 + :q = 1 : room temperature 22-25 C + q = 3 : body temperature 35 C +} + +ASSIGNED { + v (mV) + ica (mA/cm2) + eca (mV) + celsius (degC) + cai (mM) + cao (mM) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + ica = pbar*m*m*(h*a+1-a)*ghk(v, cai, cao) +} + +INITIAL { + rates() + m = minf + h = hinf +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-3))/(-8))) + mtau = 0.06+1/(exp((v-25)/18)+exp((v-(-31))/(-44))) + hinf = 1/(1+exp((v-(-74.8))/6.5)) + htau = 70 + UNITSON +} + +FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) { + LOCAL z, eci, eco + z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15)) + eco = co*efun(z) + eci = ci*efun(-z) + ghk = (1e-3)*2*FARADAY*(eci-eco) +} + +FUNCTION efun(z) { + if (fabs(z) < 1e-4) { + efun = 1-z/2 + }else{ + efun = z/(exp(z)-1) + } +} + +COMMENT + +Model is based on mixed data. Activation curve is from neostriatal +medium spiny neurons of adult P28+ rats [1, Fig.12F], unspecified +recording temperature. Potentials were not corrected for the liquid +junction potential, which was estimated to be 7 mV. Activation time +constant is from the rodent neuron culture (both rat and mouse cells), +room temperature 22-25 C [2, Fig.15B]. Inactivation data is from human +(HEK) cells [3, Tab.1, Tab.2], supposedly at room temperature. + +Kinetics of m2h type is used [2, Fig.5]. Activation of m^2 is fitted +to the experimental data [1,4], activation time constant corresponds +to m^2 already [2]. Original model [5,4] was modified by Alexander +Kozlov . Activation time constant was refitted to avoid +singularity in the expression. Temperature correction factor 3 is used +for body temperature [4,5]. + +[1] Bargas J, Howe A, Eberwine J, Cao Y, Surmeier DJ (1994) Cellular +and molecular characterization of Ca2+ currents in acutely isolated, +adult rat neostriatal neurons. J Neurosci 14(11 Pt 1):6667-86. + +[2] Kasai H, Neher E (1992) Dihydropyridine-sensitive and +omega-conotoxin-sensitive calcium channels in a mammalian +neuroblastoma-glioma cell line. J Physiol 448:161-88. + +[3] McNaughton NC, Randall AD (1997) Electrophysiological properties of +the human N-type Ca2+ channel: I. Channel gating in Ca2+, Ba2+ and Sr2+ +containing solutions. Neuropharmacology 36(7):895-915. + +[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +[5] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +ENDCOMMENT diff --git a/examples/example_models/str-fs-model1/mechanisms/caq_fs.mod b/examples/example_models/str-fs-model1/mechanisms/caq_fs.mod new file mode 100644 index 00000000..7d4efcf9 --- /dev/null +++ b/examples/example_models/str-fs-model1/mechanisms/caq_fs.mod @@ -0,0 +1,100 @@ +TITLE Q-type calcium current (Cav2.1) + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (S) = (siemens) + (molar) = (1/liter) + (mM) = (millimolar) + FARADAY = (faraday) (coulomb) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX caq_fs + USEION ca READ cai, cao WRITE ica VALENCE 2 + RANGE pbar, ica +} + +PARAMETER { + pbar = 0.0 (cm/s) + :q = 1 : room temperature 22 C + q = 3 : body temperature 35 C +} + +ASSIGNED { + v (mV) + ica (mA/cm2) + eca (mV) + celsius (degC) + cai (mM) + cao (mM) + minf + mtau (ms) +} + +STATE { m } + +BREAKPOINT { + SOLVE states METHOD cnexp + ica = pbar*m*m*ghk(v, cai, cao) +} + +INITIAL { + rates() + m = minf +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-16.3))/(-7.9))) + mtau = 1.13*2 + UNITSON +} + +FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) { + LOCAL z, eci, eco + z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15)) + if(z == 0) { + z = z+1e-6 + } + eco = co*(z)/(exp(z)-1) + eci = ci*(-z)/(exp(-z)-1) + ghk = (1e-3)*2*FARADAY*(eci-eco) +} + +COMMENT + +Activation curve was reconstructed for cultured NAc neurons from P5-P32 +Charles River rat pups [1]. Activation time constant was measured in +culture neurons from cerebellum of P2-P5 rat pups [2] at room temperature +22 C. + +Original NEURON model by Wolf (2005) [3] was modified by Alexander Kozlov +. Activation curve was fitted to m2 kinetics [4], +activation time constant was scaled up as well. + +[1] Churchill D, Macvicar BA (1998) Biophysical and pharmacological +characterization of voltage-dependent Ca2+ channels in neurons isolated +from rat nucleus accumbens. J Neurophysiol 79(2):635-47. + +[2] Randall A, Tsien RW (1995) Pharmacological dissection of multiple +types of Ca2+ channel currents in rat cerebellar granule neurons. J +Neurosci 15(4):2995-3012. + +[3] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[4] Brown AM, Schwindt PC, Crill WE (1993) Voltage dependence and +activation kinetics of pharmacologically defined components of the +high-threshold calcium current in rat neocortical neurons. J Neurophysiol +70(4):1530-43. + +ENDCOMMENT diff --git a/examples/example_models/str-fs-model1/mechanisms/car_fs.mod b/examples/example_models/str-fs-model1/mechanisms/car_fs.mod new file mode 100644 index 00000000..2bc97677 --- /dev/null +++ b/examples/example_models/str-fs-model1/mechanisms/car_fs.mod @@ -0,0 +1,113 @@ +TITLE R-type calcium current (Cav2.3) + +UNITS { + (mV) = (millivolt) + (mA) = (milliamp) + (S) = (siemens) + (molar) = (1/liter) + (mM) = (millimolar) + FARADAY = (faraday) (coulomb) + R = (k-mole) (joule/degC) +} + +NEURON { + SUFFIX car_fs + USEION ca READ cai, cao WRITE ica VALENCE 2 + RANGE pbar, ica +} + +PARAMETER { + pbar = 0.0 (cm/s) + :q = 1 : room temperature 22 C + q = 3 : body temperature 35 C +} + +ASSIGNED { + v (mV) + ica (mA/cm2) + eca (mV) + celsius (degC) + cai (mM) + cao (mM) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + ica = pbar*m*m*m*h*ghk(v, cai, cao) +} + +INITIAL { + rates() + m = minf + h = hinf +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-29))/(-9.6))) + mtau = 5.1 + hinf = 1/(1+exp((v-(-33.3))/17)) + htau = 22+80/(1+exp((v-(-19))/5)) + UNITSON +} + +FUNCTION ghk(v (mV), ci (mM), co (mM)) (.001 coul/cm3) { + LOCAL z, eci, eco + z = (1e-3)*2*FARADAY*v/(R*(celsius+273.15)) + eco = co*efun(z) + eci = ci*efun(-z) + ghk = (1e-3)*2*FARADAY*(eci-eco) +} + +FUNCTION efun(z) { + if (fabs(z) < 1e-4) { + efun = 1-z/2 + }else{ + efun = z/(exp(z)-1) + } +} + +COMMENT + +Original data by Foehring et al (2000) [1] for dissociated MSNs from +P28-P42 Sprague-Dawley rat brain. Unspecified recording temperature. The +liquid junction potential was around 8 mV and was not corrected. Kinetics +of m3h type was fitted. Inactivation time constants were measured in +neurons from endopiriform nucleus of P7-P21 Hartley guinea pigs [2] +at room temperature 22 C. + +Original NEURON model by Wolf (2005) [3] modified by Alexander Kozlov +. Activation curve fitted to m^3 kinetics as in [4], +activation time constant matched m^3 originally [1]. Smooth fit of +inactivation time constant from [2,3]. + +[1] Foehring RC, Mermelstein PG, Song WJ, Ulrich S, Surmeier DJ +(2000) Unique properties of R-type calcium currents in neocortical and +neostriatal neurons. J Neurophysiol 84(5):2225-36. + +[2] Brevi S, de Curtis M, Magistretti J (2001) Pharmacological and +biophysical characterization of voltage-gated calcium currents in the +endopiriform nucleus of the guinea pig. J Neurophysiol 85(5):2076-87. + +[3] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +ENDCOMMENT diff --git a/examples/example_models/str-fs-model1/mechanisms/kaf_fs.mod b/examples/example_models/str-fs-model1/mechanisms/kaf_fs.mod new file mode 100644 index 00000000..8f364c5c --- /dev/null +++ b/examples/example_models/str-fs-model1/mechanisms/kaf_fs.mod @@ -0,0 +1,128 @@ +TITLE Fast A-type potassium current (Kv4.2) + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + +NEURON { + SUFFIX kaf_fs + USEION k READ ek WRITE ik + RANGE gbar, gk, ik, q + RANGE modDA, maxModDA, levelDA +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.0 (S/cm2) + :q = 1 : room temperature (unspecified) + q = 3 : body temperature 35 C + modDA = 0 + maxModDA = 1 + levelDA = 0 +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + gk (S/cm2) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + gk = gbar*m*m*h*modulationDA() + ik = gk*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +INITIAL { + rates() + m = minf + h = hinf +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-10))/(-17.7))) + mtau = (0.9+1.1/(1+exp((v-(-30))/10)))*2 + hinf = 1/(1+exp((v-(-75.6))/11.8)) + htau = 14 + UNITSON +} + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + +COMMENT + +Original data by Tkatch et al (2000) [1]. Neostriatal neurons were acutely +dissociated from young adult rats, age P28-P42. Electrophysiological +recordings were done at unspecified temperature (room temperature 20-22 C +assumed). Potentials were not corrected for the liquid junction potential +(estimated 1-2 mV). + +Conductance kinetics of m2h type is used [2]. Activation m^1 matches +experimental data [1, Fig.2C]. Activation time constants were fitted to +tabulated data [1, Fig.2B] by Alexander Kozlov and scaled +up x2 for m2 kinetics. Slope of inactivation function fitted to the data +[1, Fig.3B] with half inactivation potential -75.6 mV. Temperature factor +q between 3 [2] and 1.5 [3] was used for body temperature. + +[1] Tkatch T, Baranauskas G, Surmeier DJ (2000) Kv4.2 mRNA abundance and +A-type K(+) current amplitude are linearly related in basal ganglia and +basal forebrain neurons. J Neurosci 20(2):579-88. + +[2] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[3] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH, +Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on +calcium influx and spike timing-dependent plasticity in striatal medium +spiny neurons. PLoS Comput Biol 8(4):e1002493. + +ENDCOMMENT diff --git a/examples/example_models/str-fs-model1/mechanisms/kas_fs.mod b/examples/example_models/str-fs-model1/mechanisms/kas_fs.mod new file mode 100644 index 00000000..b08ddb6b --- /dev/null +++ b/examples/example_models/str-fs-model1/mechanisms/kas_fs.mod @@ -0,0 +1,128 @@ +TITLE Slowly inactivating A-type potassium current (Kv1.2) + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + +NEURON { + SUFFIX kas_fs + USEION k READ ek WRITE ik + RANGE gbar, gk, ik, shift, q + RANGE modDA, maxModDA, levelDA +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.0 (S/cm2) + a = 0.8 + :q = 1 : room temperature 22-24 C + q = 3 : body temperature 33 C + shift = 0 + modDA = 0 + maxModDA = 1 + levelDA = 0 +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + gk (S/cm2) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + gk = gbar*m*m*(h*a+1-a)*modulationDA() + ik = gk*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +INITIAL { + rates() + m = minf + h = hinf +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-27)-shift)/(-16))) + mtau = 3.4+89.2*exp(-((v-(-34.3))/30.1)^2) + hinf = 1/(1+exp((v-(-33.5)-shift)/21.5)) + htau = 548.7*6/(exp((v-(-96))/(-29.01))+exp((v-(-96))/100)) + UNITSON +} + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + +COMMENT + +Experimental data by Shen et al (2004) [1]. Medium spiny neurons were +acutely dissociated from from young adult (P21-P28) Sprague-Dawley rat +brain. All recordings were conducted at 22-24 C. No correction for the +liquid junction potential was reported. + +Conductance kinetics of m2h type is used [1] with partial inactivation, +m2 (a h + (1-a)). Fraction a is set to 0.8, as in [1, Fig.6B]; other +values for a are possible [2] (see also kas.mod in companion code). +Equation for htau [1] is corrected to match the authors' data [1, Fig.6B] +by Alexander Kozlov . Time constants were corrected to +body temperature with factor q=3 [1,3]. + +[1] Shen W, Hernandez-Lopez S, Tkatch T, Held JE, Surmeier DJ (2004) +Kv1.2-containing K+ channels regulate subthreshold excitability of +striatal medium spiny neurons. J Neurophysiol 91(3):1337-49. + +[2] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[3] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +ENDCOMMENT diff --git a/examples/example_models/str-fs-model1/mechanisms/kdr_fs.mod b/examples/example_models/str-fs-model1/mechanisms/kdr_fs.mod new file mode 100644 index 00000000..99b8287d --- /dev/null +++ b/examples/example_models/str-fs-model1/mechanisms/kdr_fs.mod @@ -0,0 +1,80 @@ +TITLE Fast delayed rectifier potassium current (Kv3.1/3.2) + +NEURON { + SUFFIX kdr_fs + USEION k READ ek WRITE ik + RANGE gbar, gk, ik, q +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.0 (S/cm2) + :q = 1 : room temperature + q = 3 : body temperature 35 C +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + gk (S/cm2) + minf + mtau (ms) +} + +STATE { m } + +BREAKPOINT { + SOLVE states METHOD cnexp + gk = gbar*m + ik = gk*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q +} + +INITIAL { + rates() + m = minf +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-13))/(-6))) + mtau = 11.1 + UNITSON +} + +COMMENT + +Original data and model by Baranauskas et al (1999) [1] for globus +pallidus neurons from young adult rat. The temperature was not +specified. Potentials were not corrected for the liquid junction +potential, which was estimated to be 1-2 mV. + +Kinetics of m1 type is used as in [2,3]. Room temperature 20-23 C +is assumed. + +NEURON implementation by Alexander Kozlov . + +[1] Baranauskas G, Tkatch T, Surmeier DJ (1999) Delayed rectifier currents +in rat globus pallidus neurons are attributable to Kv2.1 and Kv3.1/3.2 +K(+) channels. J Neurosci 19(15):6394-404. + +[2] Migliore M, Hoffman DA, Magee JC, Johnston D (1999) Role of an +A-type K+ conductance in the back-propagation of action potentials in the +dendrites of hippocampal pyramidal neurons. J Comput Neurosci 7(1):5-15. + +[3] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH, +Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on +calcium influx and spike timing-dependent plasticity in striatal medium +spiny neurons. PLoS Comput Biol 8(4):e1002493. + +ENDCOMMENT diff --git a/examples/example_models/str-fs-model1/mechanisms/kir_fs.mod b/examples/example_models/str-fs-model1/mechanisms/kir_fs.mod new file mode 100644 index 00000000..3bd8313b --- /dev/null +++ b/examples/example_models/str-fs-model1/mechanisms/kir_fs.mod @@ -0,0 +1,130 @@ +TITLE Non-inactivating inwardly rectifying potassium current (Kir2.3) + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + +NEURON { + SUFFIX kir_fs + USEION k READ ek WRITE ik + RANGE gbar, gk, ik, shift + RANGE modDA, maxModDA, levelDA +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.0 (S/cm2) + shift = 0 + q = 1 : body temperature 35 C + modDA = 0 + maxModDA = 1 + levelDA = 0 +} + +ASSIGNED { + v (mV) + ek (mV) + ik (mA/cm2) + gk (S/cm2) + minf + mtau (ms) +} + +STATE { m } + +BREAKPOINT { + SOLVE states METHOD cnexp + gk = gbar*m*modulationDA() + ik = gk*(v-ek) +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q +} + +INITIAL { + rates() + m = minf +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-82)-shift)/13)) + mtau = 1/(exp((v-(-103)-shift)/(-14.5))+0.125/(1+exp((v-(-35)-shift)/(-19)))) + UNITSON +} + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + + +COMMENT + +Original model by Wolf et al (2005) [1] for the rat MSN cells from the +nucleus accumbens. The activation curve was fitted to a mouse Kir2.1 +channel expressed in HEK cells [2] and shifted to match extracellular +concentration of K in rat. Time constants were derived from Aplysia data +[3] and adjusted to match the rat experiments [1]. Time constant was +further tuned [4] to fit the rat data below -80 mV [5]. Kinetics is +corrected to the body temperature 35 C. + +Non-inactivating Kir current was observed in cells expressing Kir2.2 +and/or Kir2.3 [5]. Activation variable with m^1 kinetics is used [1,4]. +Smooth fit of the time constants by Alexander Kozlov . + +[1] Wolf JA, Moyer JT, Lazarewicz MT, Contreras D, Benoit-Marand M, +O'Donnell P, Finkel LH (2005) NMDA/AMPA ratio impacts state transitions +and entrainment to oscillations in a computational model of the nucleus +accumbens medium spiny projection neuron. J Neurosci 25(40):9080-95. + +[2] Kubo Y, Murata Y (2001) Control of rectification and permeation by +two distinct sites after the second transmembrane region in Kir2.1 K+ +channel. J Physiol 531, 645-660. + +[3] Hayashi H, Fishman HM (1988) Inward rectifier K+ channel kinetics +from analysis of the complex conductance of aplysia neuronal membrane. +Biophys J 53, 747-757. + +[4] Steephen JE, Manchanda R (2009) Differences in biophysical properties +of nucleus accumbens medium spiny neurons emerging from inactivation of +inward rectifying potassium currents. J Comput Neurosci 27(3):453-70 + +[5] Mermelstein PG, Song WJ, Tkatch T, Yan Z, Surmeier DJ (1998) +Inwardly rectifying potassium (IRK) currents are correlated with IRK +subunit expression in rat nucleus accumbens medium spiny neurons. J +Neurosci 18(17):6650-61. + +ENDCOMMENT diff --git a/examples/example_models/str-fs-model1/mechanisms/naf_fs.mod b/examples/example_models/str-fs-model1/mechanisms/naf_fs.mod new file mode 100644 index 00000000..3dcfc3a9 --- /dev/null +++ b/examples/example_models/str-fs-model1/mechanisms/naf_fs.mod @@ -0,0 +1,119 @@ +TITLE Fast transient sodium current + +COMMENT + +Neuromodulation is added as functions: + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA + +where: + + modDA [0]: is a switch for turning modulation on or off {1/0} + maxModDA [1]: is the maximum modulation for this specific channel (read from the param file) + e.g. 10% increase would correspond to a factor of 1.1 (100% +10%) {0-inf} + levelDA [0]: is an additional parameter for scaling modulation. + Can be used simulate non static modulation by gradually changing the value from 0 to 1 {0-1} + + Further neuromodulators can be added by for example: + modulationDA = 1 + modDA*(maxModDA-1) + modulationACh = 1 + modACh*(maxModACh-1) + .... + + etc. for other neuromodulators + + + +[] == default values +{} == ranges + +ENDCOMMENT + +NEURON { + SUFFIX naf_fs + USEION na READ ena WRITE ina + RANGE gbar, gna, ina, q + RANGE modDA, maxModDA, levelDA +} + +UNITS { + (S) = (siemens) + (mV) = (millivolt) + (mA) = (milliamp) +} + +PARAMETER { + gbar = 0.0 (S/cm2) + :q = 1 : room temperature 22 C + q = 1.8 : body temperature 35 C + modDA = 0 + maxModDA = 1 + levelDA = 0 +} + +ASSIGNED { + v (mV) + ena (mV) + ina (mA/cm2) + gna (S/cm2) + minf + mtau (ms) + hinf + htau (ms) +} + +STATE { m h } + +BREAKPOINT { + SOLVE states METHOD cnexp + gna = gbar*m*m*m*h*modulationDA() + ina = gna*(v-ena) +} + +DERIVATIVE states { + rates() + m' = (minf-m)/mtau*q + h' = (hinf-h)/htau*q +} + +INITIAL { + rates() + m = minf + h = hinf +} + +PROCEDURE rates() { + UNITSOFF + minf = 1/(1+exp((v-(-25))/(-10))) + mtau = 0.33+1/(exp((v-(-62))/14)+exp((v-(-60))/(-17))) + hinf = 1/(1+exp((v-(-62))/6)) + htau = 0.6+1/(exp((v-(-44))/8)+exp((v-(-99))/(-44))) + UNITSON +} + +FUNCTION modulationDA() { + : returns modulation factor + + modulationDA = 1 + modDA*(maxModDA-1)*levelDA +} + +COMMENT + +Original data by Ogata and Tatebayashi (1990) [1]. Neostriatal neurons +of medium size (putative medium spiny neurons) freshly isolated from +the adult guinea pig brain (either sex, 200 g). Data compensated for +the liquid junction potential (-13 mV). Experiments carried out at room +temperature (22 C). Conductance fitted by m3h kinetics. + +Smooth fit of mtau and htau data [1] by Alexander Kozlov +assuming natural logarithm of tau values [1, Figs. 5 and 9] and +temperature correction factor of 1.8-2.1 [2] as suggested by Robert +Lindroos . + +[1] Ogata N, Tatebayashi H (1990) Sodium current kinetics in freshly +isolated neostriatal neurones of the adult guinea pig. Pflugers Arch +416(5):594-603. + +[2] Schwarz JR (1986) The effect of temperature on Na currents in rat +myelinated nerve fibres. Pflugers Arch. 406(4):397-404. + +ENDCOMMENT diff --git a/examples/example_models/str-fs-model1/mechanisms/sk_fs.mod b/examples/example_models/str-fs-model1/mechanisms/sk_fs.mod new file mode 100644 index 00000000..4f2c3e34 --- /dev/null +++ b/examples/example_models/str-fs-model1/mechanisms/sk_fs.mod @@ -0,0 +1,83 @@ +TITLE SK-type calcium activated K channel (KCa2.2) + +UNITS { + (molar) = (1/liter) + (mV) = (millivolt) + (mA) = (milliamp) + (mM) = (millimolar) +} + +NEURON { + SUFFIX sk_fs + USEION ca READ cai + USEION k READ ek WRITE ik + RANGE gbar, ik +} + +PARAMETER { + gbar = 0.0 (mho/cm2) + :q = 1 : room temperature + q = 3 : body temperature +} + +ASSIGNED { + v (mV) + ik (mA/cm2) + cai (mM) + ek (mV) + oinf + otau (ms) +} + +STATE { o } + +BREAKPOINT { + SOLVE state METHOD cnexp + ik = gbar*o*(v-ek) +} + +DERIVATIVE state { + rate(v, cai) + o' = (oinf-o)/otau*q +} + +INITIAL { + rate(v, cai) + o = oinf +} + +PROCEDURE rate(v (mV), ca (mM)) { + LOCAL a + a = (ca/0.57e-3)^5.2 + oinf = a/(1+a) + otau = 4.9 +} + +COMMENT + +Experimental data was obtained for the apamin-sensitive clone rSK2 from +rat brain cDNA expressed in Xenopus oocytes [1,2]. All experiments were +performed at room tempretaure. + +Original model [3] used calcium dependence from [2, Fig.2] and calcium +activation time constant from [1, Fig.13]. NEURON implementation by +Alexander Kozlov follows the revised model [4]. + +[1] Hirschberg B, Maylie J, Adelman JP, Marrion NV (1998) Gating of +recombinant small-conductance Ca-activated K+ channels by calcium. J +Gen Physiol 111(4):565-81. + +[2] Maylie J, Bond CT, Herson PS, Lee WS, Adelman JP (2004) Small +conductance Ca2+-activated K+ channels and calmodulin. J Physiol 554(Pt +2):255-61. + +[3] Evans RC, Morera-Herreras T, Cui Y, Du K, Sheehan T, Kotaleski JH, +Venance L, Blackwell KT (2012) The effects of NMDA subunit composition on +calcium influx and spike timing-dependent plasticity in striatal medium +spiny neurons. PLoS Comput Biol 8(4):e1002493. + +[4] Evans RC, Maniar YM, Blackwell KT (2013) Dynamic modulation of +spike timing-dependent calcium influx during corticostriatal upstates. J +Neurophysiol 110(7):1631-45. + +ENDCOMMENT diff --git a/examples/example_models/fs/model2/morphology/MTC180800A-IDB-cor-rep-res3-var0.swc b/examples/example_models/str-fs-model1/morphology/MTC180800A-IDB-cor-rep-res3-var0.swc similarity index 100% rename from examples/example_models/fs/model2/morphology/MTC180800A-IDB-cor-rep-res3-var0.swc rename to examples/example_models/str-fs-model1/morphology/MTC180800A-IDB-cor-rep-res3-var0.swc diff --git a/examples/example_models/fs/model2/morphology/MTC180800A-IDB-cor-rep-res3-var1.swc b/examples/example_models/str-fs-model1/morphology/MTC180800A-IDB-cor-rep-res3-var1.swc similarity index 100% rename from examples/example_models/fs/model2/morphology/MTC180800A-IDB-cor-rep-res3-var1.swc rename to examples/example_models/str-fs-model1/morphology/MTC180800A-IDB-cor-rep-res3-var1.swc diff --git a/examples/example_models/fs/model2/morphology/MTC180800A-IDB-cor-rep-res3-var2.swc b/examples/example_models/str-fs-model1/morphology/MTC180800A-IDB-cor-rep-res3-var2.swc similarity index 100% rename from examples/example_models/fs/model2/morphology/MTC180800A-IDB-cor-rep-res3-var2.swc rename to examples/example_models/str-fs-model1/morphology/MTC180800A-IDB-cor-rep-res3-var2.swc diff --git a/examples/example_models/fs/model2/morphology/MTC180800A-IDB-cor-rep-res3-var3.swc b/examples/example_models/str-fs-model1/morphology/MTC180800A-IDB-cor-rep-res3-var3.swc similarity index 100% rename from examples/example_models/fs/model2/morphology/MTC180800A-IDB-cor-rep-res3-var3.swc rename to examples/example_models/str-fs-model1/morphology/MTC180800A-IDB-cor-rep-res3-var3.swc diff --git a/examples/example_models/fs/model2/morphology/MTC180800A-IDB-cor-rep-res3-var4.swc b/examples/example_models/str-fs-model1/morphology/MTC180800A-IDB-cor-rep-res3-var4.swc similarity index 100% rename from examples/example_models/fs/model2/morphology/MTC180800A-IDB-cor-rep-res3-var4.swc rename to examples/example_models/str-fs-model1/morphology/MTC180800A-IDB-cor-rep-res3-var4.swc diff --git a/examples/example_models/fs/model2/morphology/MTC180800A-IDB-cor-rep-res3-var5.swc b/examples/example_models/str-fs-model1/morphology/MTC180800A-IDB-cor-rep-res3-var5.swc similarity index 100% rename from examples/example_models/fs/model2/morphology/MTC180800A-IDB-cor-rep-res3-var5.swc rename to examples/example_models/str-fs-model1/morphology/MTC180800A-IDB-cor-rep-res3-var5.swc diff --git a/examples/example_models/fs/model2/morphology/MTC180800A-IDB-cor-rep-res3-var6.swc b/examples/example_models/str-fs-model1/morphology/MTC180800A-IDB-cor-rep-res3-var6.swc similarity index 100% rename from examples/example_models/fs/model2/morphology/MTC180800A-IDB-cor-rep-res3-var6.swc rename to examples/example_models/str-fs-model1/morphology/MTC180800A-IDB-cor-rep-res3-var6.swc diff --git a/examples/example_models/fs/model2/morphology/MTC180800A-IDB-cor-rep-res3-var7.swc b/examples/example_models/str-fs-model1/morphology/MTC180800A-IDB-cor-rep-res3-var7.swc similarity index 100% rename from examples/example_models/fs/model2/morphology/MTC180800A-IDB-cor-rep-res3-var7.swc rename to examples/example_models/str-fs-model1/morphology/MTC180800A-IDB-cor-rep-res3-var7.swc diff --git a/examples/example_models/fs/model2/morphology/MTC180800A-IDB-cor-rep-res3-var8.swc b/examples/example_models/str-fs-model1/morphology/MTC180800A-IDB-cor-rep-res3-var8.swc similarity index 100% rename from examples/example_models/fs/model2/morphology/MTC180800A-IDB-cor-rep-res3-var8.swc rename to examples/example_models/str-fs-model1/morphology/MTC180800A-IDB-cor-rep-res3-var8.swc diff --git a/examples/example_models/fs/model2/morphology/MTC180800A-IDB-cor-rep-res3.swc b/examples/example_models/str-fs-model1/morphology/MTC180800A-IDB-cor-rep-res3.swc similarity index 100% rename from examples/example_models/fs/model2/morphology/MTC180800A-IDB-cor-rep-res3.swc rename to examples/example_models/str-fs-model1/morphology/MTC180800A-IDB-cor-rep-res3.swc diff --git a/examples/example_models/str-fs-model1/val_models.json b/examples/example_models/str-fs-model1/val_models.json new file mode 100644 index 00000000..d12880da --- /dev/null +++ b/examples/example_models/str-fs-model1/val_models.json @@ -0,0 +1,18806 @@ +[ + { + "ephys": "160628_FS2", + "morph": "MTC180800A-IDB-cor-rep-res3-var0.swc", + "par": 0, + "iv_curve": { + "stim": [ + -391.90000000000003, + -387.62864321608043, + -383.35728643216083, + -379.08592964824123, + -374.81457286432163, + -370.54321608040203, + -366.27185929648243, + -362.00050251256283, + -357.72914572864323, + -353.45778894472363, + -349.18643216080403, + -344.91507537688443, + -340.64371859296483, + -336.37236180904523, + -332.10100502512563, + -327.8296482412061, + -323.55829145728643, + -319.2869346733669, + -315.01557788944723, + -310.7442211055277, + -306.47286432160803, + -302.2015075376885, + -297.9301507537689, + 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