diff --git a/.github/data_validation_configs/reagent_resources.json b/.github/data_validation_configs/reagent_resources.json index 6616b7b..19f0385 100644 --- a/.github/data_validation_configs/reagent_resources.json +++ b/.github/data_validation_configs/reagent_resources.json @@ -204,7 +204,8 @@ "Thymus", "Tonsil", "Tumor", - "Uterus" + "Uterus", + "Nasal Polyp" ], "Tissue State":[ "Cancer", diff --git a/.zenodo.json b/.zenodo.json index 464b5e4..29c0209 100644 --- a/.zenodo.json +++ b/.zenodo.json @@ -63,6 +63,11 @@ "name": "Croteau, Joshua", "orcid": "0000-0002-8142-0482" }, + { + "affiliation": "McMaster University", + "name": "Denha, Saven", + "orcid": "0009-0004-8162-409X" + }, { "affiliation": "National Institutes of Health", "name": "Dever, Rose", diff --git a/data/image_resources.csv b/data/image_resources.csv index e87dc36..c32598c 100644 --- a/data/image_resources.csv +++ b/data/image_resources.csv @@ -210,3 +210,5 @@ CXCR3_AF700/Human_tonsil_FFPE_CXCR3_561320.jpg,"Human tonsil FFPE: CXCR3 (cyan, CD4_AF488/Human_tonsil_FFPE_CD4_AF488_FAB8165G.jpg,"Human tonsil FFPE: CD4 (green, catalog number FAB8165G)",37fcddd186b1279f72570872be797e13,['CD4_AF488/0000-0002-6604-2065.md'] CXCR3_AF700/Human_Tonsil_FFPE_CXCR3_FAB160N.jpg,"Human tonsil FFPE: CXCR3 (cyan, catalog number FAB160N)",282723d37e8b3bfc2fe3a57fa805f7bc,['CXCR3_AF700/0000-0002-6604-2065.md'] CD20_eF615/Human_Tonsil_FFPE_CD20_eF615_42-0202-82.jpg,"Human tonsil FFPE: CD20 (red, catalog number 42-0202-82)",77e6ac7e3a9009daa030e9f17c118f0c,['CD20_eF615/0000-0002-6604-2065.md'] +E-cadherin_AF647/Ecadherin_Yellow_DAPI_Cyan_EpCAM_Blue.jpg,"Human Nasal Polyp: E-Cadherin (yellow, catalog number 147307), EpCAM (blue, catalog number 324228) and DAPI (cyan)",9b79c06f612c26ba7af3cb03c4e21298,['E-cadherin_AF647/0009-0004-8162-409X.md'] +E-cadherin_AF647/Ecadherin_Yellow_DAPI_Cyan.jpg,"Human Nasal Polyp: E-Cadherin (yellow, catalog number 147307), and DAPI (cyan)",4dc47ddf0a00a3ad872b91ba448f914a,['E-cadherin_AF647/0009-0004-8162-409X.md'] \ No newline at end of file diff --git a/data/reagent_resources.csv b/data/reagent_resources.csv index b76fca7..1979ccd 100644 --- a/data/reagent_resources.csv +++ b/data/reagent_resources.csv @@ -1181,4 +1181,5 @@ P01903,Primary Antibody,HLA-DR,Human,Mouse,IgG2a,L243,BioLegend,307622,AF647,AB_ P49682,Primary Antibody,CXCR3,Human,Mouse,IgG1,1C6/CXCR3,BD Biosciences,561320,AF700,AB_10611719,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 (Sodium Citrate buffer) for 45 minutes,NA,No,0000-0002-6604-2065,NA,0000-0002-6604-2065 P01730,Primary Antibody,CD4,Human,Goat,IgG,Polyclonal,R&D Systems,FAB8165G,AF488,AB_2728839,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 (Sodium Citrate buffer) for 45 minutes,NA,Yes,0000-0002-6604-2065,NA,0000-0002-6604-2065 P49682,Primary Antibody,CXCR3,Human,Mouse,IgG1,49801,R&D Systems,FAB160N,AF700,NA,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 (Sodium Citrate buffer) for 45 minutes,NA,No,0000-0002-6604-2065,NA,0000-0002-6604-2065 -P11836,Primary Antibody,CD20,Human,Mouse,IgG2a,L26,Thermo Fisher Scientific,42-0202-82,eF615,AB_10853517,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 (Sodium Citrate buffer) for 45 minutes,NA,Yes,0000-0002-6604-2065,NA,0000-0002-6604-2065 \ No newline at end of file +P11836,Primary Antibody,CD20,Human,Mouse,IgG2a,L26,Thermo Fisher Scientific,42-0202-82,eF615,AB_10853517,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 (Sodium Citrate buffer) for 45 minutes,NA,Yes,0000-0002-6604-2065,NA,0000-0002-6604-2065 +P12830,Primary Antibody,E-cadherin,Human,Rat,IgG1,DECMA-1,BioLegend,147307,AF647,AB_2563954,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Nasal Polyp,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0009-0004-8162-409X,NA,0009-0004-8162-409X diff --git a/docs/supporting_material/E-cadherin_AF647/0009-0004-8162-409X.md b/docs/supporting_material/E-cadherin_AF647/0009-0004-8162-409X.md new file mode 100644 index 0000000..4a20686 --- /dev/null +++ b/docs/supporting_material/E-cadherin_AF647/0009-0004-8162-409X.md @@ -0,0 +1,23 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:--------------------|:------------| +| P12830 | Primary Antibody | E-cadherin | Human | Rat | IgG1 | DECMA-1 | BioLegend | 147307 | AF647 | AB_2563954 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Nasal Polyp | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0009-0004-8162-409X](https://orcid.org/0009-0004-8162-409X) | NA | [0009-0004-8162-409X](https://orcid.org/0009-0004-8162-409X) | [1](#notes) | + +# Publications + + +# Additional Notes + + +| Human Nasal Polyp: E-Cadherin (yellow, catalog number 147307), and DAPI (cyan) | +|:-------:| +| ![](Ecadherin_Yellow_DAPI_Cyan.jpg) | + +| Human Nasal Polyp: E-Cadherin (yellow, catalog number 147307), EpCAM (blue, catalog number 324228) and DAPI (cyan) | +|:-------:| +| ![](Ecadherin_Yellow_DAPI_Cyan_EpCAM_Blue.jpg) | diff --git a/docs/supporting_material/E-cadherin_AF647/Ecadherin_Yellow_DAPI_Cyan.jpg b/docs/supporting_material/E-cadherin_AF647/Ecadherin_Yellow_DAPI_Cyan.jpg new file mode 100644 index 0000000..40cb272 Binary files /dev/null and b/docs/supporting_material/E-cadherin_AF647/Ecadherin_Yellow_DAPI_Cyan.jpg differ diff --git a/docs/supporting_material/E-cadherin_AF647/Ecadherin_Yellow_DAPI_Cyan_EpCAM_Blue.jpg b/docs/supporting_material/E-cadherin_AF647/Ecadherin_Yellow_DAPI_Cyan_EpCAM_Blue.jpg new file mode 100644 index 0000000..96ec4de Binary files /dev/null and b/docs/supporting_material/E-cadherin_AF647/Ecadherin_Yellow_DAPI_Cyan_EpCAM_Blue.jpg differ