From 2bc2146c853a4c36b151cf8c92a2aa99a575b1a8 Mon Sep 17 00:00:00 2001 From: Ziv Yaniv Date: Fri, 7 Jun 2024 18:12:08 -0400 Subject: [PATCH] Adding contributions from Yuri Lin ORCID 0009-0006-9784-2694. Adding reagent information contributed by Yuri Lin. --- .zenodo.json | 5 ++++ data/image_resources.csv | 7 +++++ data/reagent_resources.csv | 15 +++++++---- .../CD34_PE/0009-0006-9784-2694.md | 22 ++++++++++++++++ ...an_Tonsil_FFPE_Hoechst_blue_CD34_green.jpg | Bin 0 -> 96145 bytes .../CD3_AF555/0000-0003-4379-8967.md | 2 +- .../CD3_AF555/0009-0006-9784-2694.md | 23 +++++++++++++++++ ...man_Tonsil_FFPE_Hoechst_blue_CD3_green.jpg | Bin 0 -> 196926 bytes .../CD45_AF488/0009-0006-9784-2694.md | 22 ++++++++++++++++ ...sil_FFPE_Hoechst_blue_CD45_AF488_green.jpg | Bin 0 -> 137599 bytes .../CD45_PE/0009-0006-9784-2694.md | 22 ++++++++++++++++ ...Tonsil_FFPE_Hoechst_blue_CD45_PE_green.jpg | Bin 0 -> 199995 bytes .../CD8_AF647/0000-0003-4379-8967.md | 2 +- .../CD8_AF647/0009-0006-9784-2694.md | 24 ++++++++++++++++++ ...Human_Tonsil_FFPE_Hoechst_blue_CD8_red.jpg | Bin 0 -> 104478 bytes .../CTLA4_AF647/0009-0006-9784-2694.md | 22 ++++++++++++++++ ...man_Tonsil_FFPE_Hoechst_blue_CTLA4_red.jpg | Bin 0 -> 116998 bytes .../PD-1_AF555/0009-0006-9784-2694.md | 22 ++++++++++++++++ ...man_Tonsil_FFPE_Hoechst_blue_PD1_green.jpg | Bin 0 -> 160074 bytes 19 files changed, 181 insertions(+), 7 deletions(-) create mode 100644 docs/supporting_material/CD34_PE/0009-0006-9784-2694.md create mode 100644 docs/supporting_material/CD34_PE/Human_Tonsil_FFPE_Hoechst_blue_CD34_green.jpg create mode 100644 docs/supporting_material/CD3_AF555/0009-0006-9784-2694.md create mode 100644 docs/supporting_material/CD3_AF555/Human_Tonsil_FFPE_Hoechst_blue_CD3_green.jpg create mode 100644 docs/supporting_material/CD45_AF488/0009-0006-9784-2694.md create mode 100644 docs/supporting_material/CD45_AF488/Human_Tonsil_FFPE_Hoechst_blue_CD45_AF488_green.jpg create mode 100644 docs/supporting_material/CD45_PE/0009-0006-9784-2694.md create mode 100644 docs/supporting_material/CD45_PE/Human_Tonsil_FFPE_Hoechst_blue_CD45_PE_green.jpg create mode 100644 docs/supporting_material/CD8_AF647/0009-0006-9784-2694.md create mode 100644 docs/supporting_material/CD8_AF647/Human_Tonsil_FFPE_Hoechst_blue_CD8_red.jpg create mode 100644 docs/supporting_material/CTLA4_AF647/0009-0006-9784-2694.md create mode 100644 docs/supporting_material/CTLA4_AF647/Human_Tonsil_FFPE_Hoechst_blue_CTLA4_red.jpg create mode 100644 docs/supporting_material/PD-1_AF555/0009-0006-9784-2694.md create mode 100644 docs/supporting_material/PD-1_AF555/Human_Tonsil_FFPE_Hoechst_blue_PD1_green.jpg diff --git a/.zenodo.json b/.zenodo.json index e090f7c..a1424d1 100644 --- a/.zenodo.json +++ b/.zenodo.json @@ -127,6 +127,11 @@ "name": "Lamborn, Ian", "orcid": "0009-0006-1490-3667" }, + { + "affiliation": "National Institutes of Health", + "name": "Lin, Yuri", + "orcid": "0009-0006-9784-2694" + }, { "affiliation": "National Institutes of Health", "name": "Makranz, Chen", diff --git a/data/image_resources.csv b/data/image_resources.csv index 75a334c..9abf676 100644 --- a/data/image_resources.csv +++ b/data/image_resources.csv @@ -157,3 +157,10 @@ B220_AF488/Mouse_LN_B220_cyan_BD_557669_CD3_red_BioLegend_100240.jpg,"Mouse lymp CD35_BV510/Mouse_LN_CD35_yellow_BD_740132_ColIV_blue_ab19808_A-11009.jpg,"Mouse lymph node: CD35 (yellow, catalog number 740132) and Collagen IV (blue, catalog number ab19808 and A-11009)",ceb4fd8cd2cc42201659aa8226b9f07a,"['CD35_BV510/0000-0003-1118-7432.md','Collagen_IV_Unconjugated/0000-0003-1118-7432.md','Rabbit_IgG__H+L__AF532/0000-0003-1118-7432.md']" CD169_PE/Mouse_LN_CD169_green_BioLegend_142404_Ki67_magenta_BD_561277.jpg,"Mouse lymph node: CD169 (green, catalog number 142404) and Ki-67 (magenta, catalog number 561277)",8a6567cde9020c3f7cf4baa8a32666f2,"['Ki-67_AF700/0000-0003-1118-7432.md','CD169_PE/0000-0003-1118-7432.md']" PD-1_BV421/Mouse_LN_PD1_cyan_135218_CD11c_magenta_117312.jpg,"Mouse lymph node: PD-1 (cyan, catalog number 135218) and CD11c (magenta, catalog number 117312)",28a7b8aa0b9b5764fe0061fb69312229,"['CD11c_AF647/0000-0003-1118-7432.md','PD-1_BV421/0000-0003-1118-7432.md']" +CD34_PE/Human_Tonsil_FFPE_Hoechst_blue_CD34_green.jpg,"Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CD34 (green, Thermo Fisher Scientific cat. no. MA1-10205)",c76364415c790451b7a0d30a401b5132,"['CD34_PE/0009-0006-9784-2694.md']" +CD3_AF555/Human_Tonsil_FFPE_Hoechst_blue_CD3_green.jpg,"Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CD3 (green, Abcam cat. no. ab208514)",bdacb094d7c4f19d787ad3231b261d1e,"['CD3_AF555/0009-0006-9784-2694.md']" +CD45_AF488/Human_Tonsil_FFPE_Hoechst_blue_CD45_AF488_green.jpg,"Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CD45 (green, Novus Biologicals cat. no. NBP2-34528AF488)",5e5dfd4b6720c2abba3f740ca32a35e7,"['CD45_AF488/0009-0006-9784-2694.md']" +CD45_PE/Human_Tonsil_FFPE_Hoechst_blue_CD45_PE_green.jpg,"Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CD45 (green, Novus Biologicals cat. no. NBP2-34528PE)",41b039e9d46868bc4769f264d795effb,"['CD45_PE/0009-0006-9784-2694.md']" +CD8_AF647/Human_Tonsil_FFPE_Hoechst_blue_CD8_red.jpg,"Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CD8 (red, BioLegend cat. no. 372906)",e2a8ea56916b2337125ea81c18050771,"['CD8_AF647/0009-0006-9784-2694.md']" +CTLA4_AF647/Human_Tonsil_FFPE_Hoechst_blue_CTLA4_red.jpg,"Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CTLA4 (red, Abcam cat. no. ab270127)",0319ef9d4863816c1d28cfa5e3ec8258,"['CTLA4_AF647/0009-0006-9784-2694.md']" +PD-1_AF555/Human_Tonsil_FFPE_Hoechst_blue_PD1_green.jpg,"Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and PD-1 (green, Abcam cat. no. ab275126)",32a9ea3243d06baf17be375554c5a9d7,"['PD-1_AF555/0009-0006-9784-2694.md']" diff --git a/data/reagent_resources.csv b/data/reagent_resources.csv index 118583e..faa9282 100644 --- a/data/reagent_resources.csv +++ b/data/reagent_resources.csv @@ -732,7 +732,7 @@ Q13509,Primary Antibody,Tubulin beta-3 chain,Mouse,Mouse,IgG2a,TUJ1,BioLegend,80 P22646,Primary Antibody,CD3,Mouse,Rat,IgG2b,17A2,BioLegend,100210,AF488,AB_389301,Stock,IBEX2D Manual,"AntigenFix (2h, 4 degrees) Fixed Frozen",Kidney,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0002-7422-3237,NA,0000-0002-7422-3237 P24788,Primary Antibody,CD11b,Mouse,Rat,IgG2b,M1/70,BioLegend,101208,PE,AB_312791,Stock,IBEX2D Manual,"AntigenFix (2h, 4 degrees) Fixed Frozen",Kidney,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0002-7422-3237,NA,0000-0002-7422-3237 Q9QXH4,Primary Antibody,CD11c,Mouse,Armenian Hamster,IgG,N418,BioLegend,117308,PE,AB_313777,Stock,IBEX2D Manual,"AntigenFix (2h, 4 degrees) Fixed Frozen",Kidney,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0002-7422-3237,NA,0000-0002-7422-3237 -A0A0B4J1G0,Primary Antibody,CD16.2,Mouse,Armenian Hamster,IgG,9.00E+09,BioLegend,149503,PE,AB_2565810,Stock,IBEX2D Manual,"AntigenFix (2h, 4 degrees) Fixed Frozen",Kidney,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0002-7422-3237,NA,0000-0002-7422-3237 +A0A0B4J1G0,Primary Antibody,CD16.2,Mouse,Armenian Hamster,IgG,9E9,BioLegend,149503,PE,AB_2565810,Stock,IBEX2D Manual,"AntigenFix (2h, 4 degrees) Fixed Frozen",Kidney,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0002-7422-3237,NA,0000-0002-7422-3237 P08101,Primary Antibody,CD16/32,Mouse,Goat,IgG,Polyclonal,R&D Systems,AF1460-SP,Unconjugated,NA,Stock,IBEX2D Manual,"AntigenFix (2h, 4 degrees) Fixed Frozen",Kidney,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0002-7422-3237,NA,0000-0002-7422-3237 P26151,Primary Antibody,CD64,Mouse,Mouse,IgG1,X54-5/7.1,BioLegend,139304,PE,AB_10612740,Stock,IBEX2D Manual,"AntigenFix (2h, 4 degrees) Fixed Frozen",Kidney,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0002-7422-3237,NA,0000-0002-7422-3237 Q08481,Primary Antibody,CD31,Mouse,Rat,IgG2a,MEC13.3,BD Biosciences,553373,PE,AB_394819,Stock,IBEX2D Manual,"AntigenFix (2h, 4 degrees) Fixed Frozen",Kidney,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0002-7422-3237,NA,0000-0002-7422-3237 @@ -963,9 +963,9 @@ P20023,Primary Antibody,CD21,Human,Rabbit,IgG,SP186,Abcam,ab306325,PE,NA,Stock,C P56199,Primary Antibody,CD49a,Human,Mouse,IgG1,CL7207,Abcam,ab243032,Unconjugated,NA,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P56199,Primary Antibody,CD49a,Human,Mouse,IgG1,CL7207,Abcam,ab243032,Unconjugated,NA,Stock,Cell DIVE-IBEX,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 NA,Secondary Antibody,Fluorescein/Oregon Green,Human,Rabbit,IgG,Polyclonal,Thermo Fisher Scientific,A-11090,AF488,AB_221562,Stock,Multiplexed 2D Imaging,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 -P07766,Primary Antibody,CD3,Human,Rabbit,IgG,EP4426,Abcam,ab208514,AF555,AB_443425,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +P07766,Primary Antibody,CD3,Human,Rabbit,IgG,EP4426,Abcam,ab208514,AF555,AB_443425,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967;0009-0006-9784-2694,NA,0000-0003-4379-8967 P07766,Primary Antibody,CD3,Human,Rabbit,IgG,EP4426,Abcam,ab208514,AF555,AB_443425,Stock,Cell DIVE-IBEX,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 -P01732,Primary Antibody,CD8,Human,Mouse,IgG1,C8/144B,BioLegend,372906,AF647,AB_2650712,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 +P01732,Primary Antibody,CD8,Human,Mouse,IgG1,C8/144B,BioLegend,372906,AF647,AB_2650712,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967;0009-0006-9784-2694,NA,0000-0003-4379-8967 P01732,Primary Antibody,CD8,Human,Mouse,IgG1,C8/144B,BioLegend,372906,AF647,AB_2650712,Stock,Cell DIVE-IBEX,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P01730,Primary Antibody,CD4,Human,Rabbit,IgG,EPR6855,Abcam,ab196372,AF488,AB_2889191,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P01730,Primary Antibody,CD4,Human,Rabbit,IgG,EPR6855,Abcam,ab196372,AF488,AB_2889191,Stock,Cell DIVE-IBEX,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 @@ -984,7 +984,7 @@ Q8TDQ0,Primary Antibody,Tim-3,Human,Rat,IgG2a,344823,R&D Systems,MAB2365,Unconju P11836,Primary Antibody,CD20,Human,Mouse,IgG2a,L26,Thermo Fisher Scientific,42-0202-82,eF615,AB_10853517,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,No,0000-0003-4379-8967,NA,0000-0003-4379-8967 P13591,Primary Antibody,CD56,Human,Mouse,IgG1,123C3.D5,Novus Biologicals,NBP2-33132AF647,AF647,NA,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,No,0000-0003-4379-8967,NA,0000-0003-4379-8967 Q9BZS1,Primary Antibody,FOXP3,Human,Mouse,IgG1,236A/E7,Thermo Fisher Scientific,41-4777-82,eF570,AB_2573609,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,No,0000-0003-4379-8967,NA,0000-0003-4379-8967 -P01106,Primary Antibody,c-Myc,Human,Mouse,IgG1,9.00E+10,Novus Biologicals,NB600-302IR,DL755,NA,Stock,Multiplexed 2D Imaging,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,No,0000-0003-4379-8967,NA,0000-0003-4379-8967 +P01106,Primary Antibody,c-Myc,Human,Mouse,IgG1,9E10,Novus Biologicals,NB600-302IR,DL755,NA,Stock,Multiplexed 2D Imaging,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,No,0000-0003-4379-8967,NA,0000-0003-4379-8967 P01903,Primary Antibody,HLA-DR,Human,Mouse,IgG2b,LN-3,Novus Biologicals,NBP2-47670AF488,AF488,NA,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0000-0003-4379-8967,NA,0000-0003-4379-8967 P01903,Primary Antibody,HLA-DR,Human,Mouse,IgG2b,LN-3,Novus Biologicals,NBP2-47670AF750,AF750,NA,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,No,0000-0003-4379-8967,NA,0000-0003-4379-8967 P15104,Primary Antibody,Glutamine synthetase,Human,Rabbit,IgG,EPR13022(B),Abcam,ab302584,AF488,NA,Stock,Multiplexed 2D Imaging,FFPE,Lymph Node,Follicular Lymphoma,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,No,0000-0003-4379-8967,NA,0000-0003-4379-8967 @@ -1086,4 +1086,9 @@ P62736,Primary Antibody,Alpha smooth muscle actin,Human,Mouse,IgG2a,1A4,Thermo F P60709,Cell Structure Reagent,Phalloidin,Human,NA,NA,NA,AAT Bioquest,23122,iF594,NA,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Retina,NA,0.1% Saponin,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-2088-8310,NA,0000-0003-2088-8310 NA,Lectin,PNA,Human,NA,NA,NA,Thermo Fisher Scientific,L21409,AF488,NA,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Retina,NA,0.1% Saponin,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-2088-8310,NA,0000-0003-2088-8310 NA,Lectin,Isolectin GS-IB4,Human,NA,NA,NA,Thermo Fisher Scientific,I21412,AF568,NA,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Retina,NA,0.1% Saponin,NA,NA,Yes,0000-0003-2088-8310,NA,0000-0003-2088-8310 -NA,Secondary Antibody,Rabbit IgG (H+L),Mouse,Goat,IgG,Polyclonal,Thermo Fisher Scientific,A-11009,AF532,AB_2534076,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-1118-7432,NA,0000-0003-1118-7432 \ No newline at end of file +NA,Secondary Antibody,Rabbit IgG (H+L),Mouse,Goat,IgG,Polyclonal,Thermo Fisher Scientific,A-11009,AF532,AB_2534076,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Lymph Node,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0000-0003-1118-7432,NA,0000-0003-1118-7432 +P28906,Primary Antibody,CD34,Human,Mouse,IgG1,QBEND/10,Thermo Fisher Scientific,MA1-10205,PE,AB_11152571,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0009-0006-9784-2694,NA,0009-0006-9784-2694 +P08575,Primary Antibody,CD45,Human,Mouse,IgG1,2B11+PD7/26,Novus Biologicals,NBP2-34528PE,PE,NA,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0009-0006-9784-2694,NA,0009-0006-9784-2694 +P08575,Primary Antibody,CD45,Human,Mouse,IgG1,2B11+PD7/26,Novus Biologicals,NBP2-34528AF488,AF488,NA,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,No,0009-0006-9784-2694,NA,0009-0006-9784-2694 +P16410,Primary Antibody,CTLA4,Human,Rabbit,IgG,CAL49,Abcam,ab270127,AF647,NA,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0009-0006-9784-2694,NA,0009-0006-9784-2694 +Q15116,Primary Antibody,PD-1,Human,Rabbit,IgG,EPR4877(2),Abcam,ab275126,AF555,AB_2728811,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond,NA,Yes,0009-0006-9784-2694,NA,0009-0006-9784-2694 diff --git a/docs/supporting_material/CD34_PE/0009-0006-9784-2694.md b/docs/supporting_material/CD34_PE/0009-0006-9784-2694.md new file mode 100644 index 0000000..6f571e9 --- /dev/null +++ b/docs/supporting_material/CD34_PE/0009-0006-9784-2694.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:-------------------------|:-----------------|:------------|:------------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:------------------|:-------------------------------------------------------------------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| +| P28906 | Primary Antibody | CD34 | Human | Mouse | IgG1 | QBEND/10 | Thermo Fisher Scientific | MA1-10205 | PE | AB_11152571 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0009-0006-9784-2694](https://orcid.org/0009-0006-9784-2694) | NA | [0009-0006-9784-2694](https://orcid.org/0009-0006-9784-2694) | [1](#notes) | + +# Publications + + + +# Additional Notes + + +1. used AF546 preset setting to capture PE signal. RBCs are visible in the snapshot and difficult to differentiate. + +| Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CD34 (green, Thermo Fisher Scientific cat. no. 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| [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[2](#publications), [1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | | P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | IBEX2D Manual | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 40 minutes at 95C (AR6 Akoya Biosciences AR600250ML) | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [2](#notes) | | P04234 | Primary Antibody | CD3 | Human | Mouse | IgG1 | UCHT1 | BioLegend | 300401 (Unconjugated) | AF555 | NA | Custom | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | NA | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [3](#notes) | -| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [2](#notes) | +| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967), [0009-0006-9784-2694](https://orcid.org/0009-0006-9784-2694) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [2](#notes) | | P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [4](#notes) | | P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [5](#notes) | | P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Cell DIVE-IBEX | FFPE | Lymph Node | Follicular Lymphoma | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[3](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | diff --git a/docs/supporting_material/CD3_AF555/0009-0006-9784-2694.md b/docs/supporting_material/CD3_AF555/0009-0006-9784-2694.md new file mode 100644 index 0000000..0a6b727 --- /dev/null +++ b/docs/supporting_material/CD3_AF555/0009-0006-9784-2694.md @@ -0,0 +1,23 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------|:-----------------|:------------|:----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:------------------|:-------------------------------------------------------------------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| P07766 | Primary Antibody | CD3 | Human | Rabbit | IgG | EP4426 | Abcam | ab208514 | AF555 | AB_443425 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967), [0009-0006-9784-2694](https://orcid.org/0009-0006-9784-2694) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | + +# Publications + + + +# Additional Notes + + + + + +| Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CD3 (green, Abcam cat. no. ab208514) | +|:-------:| +| ![](Human_Tonsil_FFPE_Hoechst_blue_CD3_green.jpg) | diff --git a/docs/supporting_material/CD3_AF555/Human_Tonsil_FFPE_Hoechst_blue_CD3_green.jpg b/docs/supporting_material/CD3_AF555/Human_Tonsil_FFPE_Hoechst_blue_CD3_green.jpg new file mode 100644 index 0000000000000000000000000000000000000000..696fc96e0302f3e4958a0b7c5b204aaaec950598 GIT binary patch literal 196926 zcmbTccT^Kf`1culk*WlP6oJq|r3Dh2fV703LPu#*LXpsm1q6{2LT@1;ErifPkfvTx zIs^~`B2APg(v*4;@4c_T-97J~vw!XG%$dLDIdkSSGv}Fkp85Va|KBQr4`Bi~0WdHy z0L=bWz<-N?I{>~*Ts&Nyd_3Gdm-+dwfaI=$1O-7VU};e~Ej3*om>N`5-^|5I-^kur z6KWG^Yk$wf$Ja;C5*Hop73Ff@$Mb&%Vc@@f83Y8~x_0fBryw2Z8r zyn;GJLlX+qx?^N)Vrm9QpwJjQdk04+Pp|vlKE8g~(6I1`$f)R;gv4h_&y!P9vvYFu 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a/docs/supporting_material/CD45_AF488/0009-0006-9784-2694.md b/docs/supporting_material/CD45_AF488/0009-0006-9784-2694.md new file mode 100644 index 0000000..5034436 --- /dev/null +++ b/docs/supporting_material/CD45_AF488/0009-0006-9784-2694.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:------------------|:-----------------|:------------|:-------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:------------------|:-------------------------------------------------------------------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| +| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | 2B11+PD7/26 | Novus Biologicals | NBP2-34528AF488 | AF488 | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | No | [0009-0006-9784-2694](https://orcid.org/0009-0006-9784-2694) | NA | [0009-0006-9784-2694](https://orcid.org/0009-0006-9784-2694) | [1](#notes) | + +# Publications + + + +# Additional Notes + + +1. No specific signal seen, only background and autofluorescent RBCs visible. + +| Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CD45 (green, Novus Biologicals cat. no. 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0000000..8304866 --- /dev/null +++ b/docs/supporting_material/CD45_PE/0009-0006-9784-2694.md @@ -0,0 +1,22 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:------------------|:-----------------|:------------|:-------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:------------------|:-------------------------------------------------------------------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| P08575 | Primary Antibody | CD45 | Human | Mouse | IgG1 | 2B11+PD7/26 | Novus Biologicals | NBP2-34528PE | PE | NA | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0009-0006-9784-2694](https://orcid.org/0009-0006-9784-2694) | NA | [0009-0006-9784-2694](https://orcid.org/0009-0006-9784-2694) | | + +# Publications + + + +# Additional Notes + + + + +| Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CD45 (green, Novus Biologicals cat. no. NBP2-34528PE) | +|:-------:| +| ![](Human_Tonsil_FFPE_Hoechst_blue_CD45_PE_green.jpg) | diff --git a/docs/supporting_material/CD45_PE/Human_Tonsil_FFPE_Hoechst_blue_CD45_PE_green.jpg b/docs/supporting_material/CD45_PE/Human_Tonsil_FFPE_Hoechst_blue_CD45_PE_green.jpg new file mode 100644 index 0000000000000000000000000000000000000000..245e14227fc04b7a521ba01aa395e9a125b3fdfa GIT binary patch literal 199995 zcmbTdcT|&0^!FQj5l}jzNeL||hc2NgAOr%^Qs^oWibO$3C<5Z4NDl}hp@h&05SsLk ziqer@1O!CsARt}PbI$AU-n-tr*8S_=JF}j@o>{ZzGqaw(XYc)e{+s)61#ry_3WEaZ z=;#2Ze>&j5&w#stYgah9IM}aoadPo+U*i>$7Zwr_5K@tr5tG+e)6<2hY2DH{b+*(u zvNOJ=W$j~Q_t4Gjk=Jbt{}4a-XU-m8=>M67j+=)^h+pV0Vd1~fhFXT`|Ih8ecK~iy z##+W&20AGKJvSW#H{E|dfa?GN9n+uH{?CH{uSQ4Dz{teR!pg>e<^iw5*&~QQy$m)ZEhA*8cuOPj6rUz~Ip2)bz~kr@8rsuisYJ);Bh{ zws#JXj!#a{&VOE9{tp)&fZ_kd`rl;#4=(OMT=a~L42&%Q!$n6Q{O4idW@M67VdgQg zVR7~6l~RplWi3)W53O_z9o=~<*wA0%4}M?BSCB_q-^`c(Jh&lpF!EClxNs_cj5 zO}}v&{H}xe%BJ+SRVj>|b#TBCiqZdQp4WwWgwT@H4K7PEUV5bhI~lPEikejcN)WVq 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default | UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:-----------------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------------------------------------------------------------------|:------------------------------|:------------|:-----------------------------------------|:-----------|:--------------------|:------------| | P01731 | Primary Antibody | CD8 | Mouse | Rat | IgG2a | 53-6.7 | BioLegend | 100724 | AF647 | AB_389326 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lung | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | -| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [2](#notes) | +| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967), [0009-0006-9784-2694](https://orcid.org/0009-0006-9784-2694) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [2](#notes) | | P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | Cell DIVE-IBEX | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [3](#notes) | | P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | Cell DIVE-IBEX | FFPE | Lung | Cancer | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | 1 mg/ml LiBH4 15 minutes | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [4](#notes) | diff --git a/docs/supporting_material/CD8_AF647/0009-0006-9784-2694.md b/docs/supporting_material/CD8_AF647/0009-0006-9784-2694.md new file mode 100644 index 0000000..847d1e7 --- /dev/null +++ b/docs/supporting_material/CD8_AF647/0009-0006-9784-2694.md @@ -0,0 +1,24 @@ +--- +layout: default +--- + +# Configurations + +| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:------------------|:-------------------------------------------------------------------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:------------| +| P01732 | Primary Antibody | CD8 | Human | Mouse | IgG1 | C8/144B | BioLegend | 372906 | AF647 | AB_2650712 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967), [0009-0006-9784-2694](https://orcid.org/0009-0006-9784-2694) | NA | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | + +# Publications + + + +# Additional Notes + + +1. used with the secondary antibody below in line 5 + + + +| Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CD8 (red, BioLegend cat. no. 372906) | +|:-------:| +| ![](Human_Tonsil_FFPE_Hoechst_blue_CD8_red.jpg) | diff --git a/docs/supporting_material/CD8_AF647/Human_Tonsil_FFPE_Hoechst_blue_CD8_red.jpg b/docs/supporting_material/CD8_AF647/Human_Tonsil_FFPE_Hoechst_blue_CD8_red.jpg new file mode 100644 index 0000000000000000000000000000000000000000..c42bde131dbbf759c27b57fb18c3a73c6b08d13f GIT binary patch literal 104478 zcmbTdcT`hZ|2`VJpnyoC0f9llKwwl#0tv;T1`xX0TGP>DM26v zq@)5tK~U-pid2n22vtO-N>N9OzUs{T{`lT|*YB=%|GM|?v(8^fG03fR&Bc~zr-{*i|000?<{n7r@n< zhql82Ss6JwS$R1H1$p`X-q-e@1LQRnj-0S?Qq;tUDH%|JmYIb$2MnE?pMnVEe;HYY zCub?E96bis(mn|>Hi4SL;MO(>q^*mq8_L}SjUoE_`3IZ}42p=1ijIjTk*O({Q`6Ej 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Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | +|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------|:-----------------|:------------|:-----------|:---------------|:-----------------------|:----------------------|:----------------|:---------------|:------------------|:-------------------------------------------------------------------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| +| Q15116 | Primary Antibody | PD-1 | Human | Rabbit | IgG | EPR4877(2) | Abcam | ab275126 | AF555 | AB_2728811 | Stock | Multiplexed 2D Imaging | FFPE | Tonsil | NA | 0.3% Triton-X-100 | pH 6 for 30 minutes ER1 (AR9961) and pH 9 for 30 minutes ER2 (AR9640) using the Leica Bond | NA | Yes | [0009-0006-9784-2694](https://orcid.org/0009-0006-9784-2694) | NA | [0009-0006-9784-2694](https://orcid.org/0009-0006-9784-2694) | | + +# Publications + + + +# Additional Notes + + + + +| Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and PD-1 (green, Abcam cat. no. ab275126) | +|:-------:| +| ![](Human_Tonsil_FFPE_Hoechst_blue_PD1_green.jpg) | diff --git a/docs/supporting_material/PD-1_AF555/Human_Tonsil_FFPE_Hoechst_blue_PD1_green.jpg b/docs/supporting_material/PD-1_AF555/Human_Tonsil_FFPE_Hoechst_blue_PD1_green.jpg new file mode 100644 index 0000000000000000000000000000000000000000..242b07cb7545b871560fbdf978a0f7b98d1016ec GIT binary patch literal 160074 zcmbTdcT`hd^fnl3AW{TD1B4TcO6gYbhtSAaTe;%wNBPXt?t){1oP=jk3n7N<~ zZa5fg!EFL;9d3De`*`bH;iH1QB3>QjxE^g47Q-hYX09F<@Hdb~v 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