From 2fb4b69c5adb82a75ac7d40e24701c2a44e7ffa1 Mon Sep 17 00:00:00 2001 From: Ziv Yaniv Date: Mon, 8 Jul 2024 17:18:35 -0400 Subject: [PATCH] Adding explanation to supporting material. Per the contributors comments on the discussion forum (https://github.com/IBEXImagingCommunity/ibex_imaging_knowledge_base/discussions/174#discussioncomment-9991522). --- docs/supporting_material/CD4_BV510/0000-0002-1461-0999.md | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/docs/supporting_material/CD4_BV510/0000-0002-1461-0999.md b/docs/supporting_material/CD4_BV510/0000-0002-1461-0999.md index 57e73b9..e5c7def 100644 --- a/docs/supporting_material/CD4_BV510/0000-0002-1461-0999.md +++ b/docs/supporting_material/CD4_BV510/0000-0002-1461-0999.md @@ -6,7 +6,7 @@ layout: default | UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes | |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:---------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------| -| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BioLegend | 100449 | BV510 | AB_2564587 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | | +| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BioLegend | 100449 | BV510 | AB_2564587 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) | # Publications @@ -20,3 +20,5 @@ layout: default | Mouse lymph node: CD4 (grey, catalog number 100443) | |:-------:| | ![](CD4_BV510_Grey_Mouse_LN.jpg) | + +1. The large cells with cytosolic signal in the bottom left of the image do not have the morphology of a CD4+ T cell. This is likely autofluorescence, and it seems to interfere with many dimmer stains on BV510. \ No newline at end of file