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Mass spectrometry

Joaquín Abián, ProteoRed, Instituto de Investigaciones Biomédicas de Barcelona

Thursday, August 3.

Proteomics: an analytical challenge

There are no PCR-like techniques for proteins.

Proteomics is more complex than transcriptomics.

Protein levels are very different.

## What? Highly precise determination of molecular masses of molecules and its aggregates.

How?

Produces gas-phase ions of the molecules. Determines the mass/charge ratio of these ions through their interaction with electric, magnetic or RF fields.

A mass spectrometer is a precision balance (scale) for molecules.