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Please always provide the complete log file |
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I am trying to construct the chloroplast genome using get_organelle, which worked for most of the pair ended reads (2*150bp), however gave the error as below to some of the cp_genome assemblies:
ERROR:
Traceback (most recent call last):
File "../miniconda3/bin/get_organelle_from_reads.py", line 3941, in main
seed_fq, seed_sam, new_seed_f = making_seed_reads_using_mapping(
File "../miniconda3/bin/get_organelle_from_reads.py", line 3040, in making_seed_reads_using_mapping
map_with_bowtie2(seed_file=seed_file, original_fq_files=original_fq_files,
File "../miniconda3/lib/python3.9/site-packages/GetOrganelleLib/pipe_control_func.py", line 413, in map_with_bowtie2
raise Exception("")
Exception
How could I deal with this error? The code I used (in HPC) is:
get_organelle_from_reads.py -t 4000 -s seed_sample.fasta -1 A101_1_pair_out -2 A101_2_pair_out -u A101_1_unpair_out,A101_2_unpair_out -o A101_plastome -R 1000 --reduce-reads-for-coverage inf --max-reads inf -F embplant_pt
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