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zymo reference #2

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hasindu2008 opened this issue Aug 2, 2023 · 3 comments
Open

zymo reference #2

hasindu2008 opened this issue Aug 2, 2023 · 3 comments

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@hasindu2008
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Thanks (again) for this 5KHz data set, which is being very useful in testing our POD5<->S/BLOW5 converter https://github.com/Psy-Fer/blue-crab,
Are these the correct zymo references that we should use with this dataset?
https://s3.amazonaws.com/zymo-files/BioPool/ZymoBIOMICS.STD.refseq.v2.zip

https://files.zymoresearch.com/protocols/_d6300_zymobiomics_microbial_community_standard.pdf
@Kirk3gaard
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Hi @hasindu2008
They are still the newest released ones yes. However, I have used the pacbio hifi based ones that zymo sent me personally but for some reason have not made public yet. I have asked them several times about updating their references.
Best regards
Rasmus

@hasindu2008
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The identity scores against the one I linked above were pretty good, so I guess they are quite close :D

@Kirk3gaard
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Yes they are pretty good but ONT data is now sufficiently good that even tiny errors matter for benchmarking 😃

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@Kirk3gaard @hasindu2008