The As0.output.json file was created with the following command:
repp make seq -i As0.input.fa -o As0.output.json --dbs addgene --settings twist.yaml -vWhich approximates to asking REPP to:
- make a plasmid sequence that's in
As0.input.fa - output build instructions in JSON to
As0.output.json - use source fragments from Addgene
- use synthesis settings from
twist.yaml - be verbose during the build