diff --git a/CHANGELOG.md b/CHANGELOG.md
index e6d6519..ff2c2f0 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -10,6 +10,10 @@
 > - 🏠 Internal
 > - 💅 Polish
 
+## Unreleased
+
+* 🐛 Fix `ReadableStream` to match TypeScript's `AsyncIterable<R>` type. ([#141](https://github.com/MattiasBuelens/web-streams-polyfill/issues/141), [#142](https://github.com/MattiasBuelens/web-streams-polyfill/pull/142))
+
 ## 3.3.2 (2024-01-04)
 
 * 🐛 Fix bad publish to npm.
diff --git a/dist/types/ts3.6/polyfill.d.ts b/dist/types/ts3.6/polyfill.d.ts
index 93ea710..1e24288 100644
--- a/dist/types/ts3.6/polyfill.d.ts
+++ b/dist/types/ts3.6/polyfill.d.ts
@@ -6,7 +6,7 @@ import type { ReadableStreamAsyncIterator, ReadableStreamIteratorOptions } from
 export * from './ponyfill';
 
 declare global {
-  interface ReadableStream<R = any> {
+  interface ReadableStream<R = any> extends AsyncIterable<R> {
     /**
      * Asynchronously iterates over the chunks in the stream's internal queue.
      *
@@ -23,7 +23,6 @@ declare global {
     /**
      * {@inheritDoc ReadableStream.values}
      */
-    [Symbol.asyncIterator]: (options?: ReadableStreamIteratorOptions) => ReadableStreamAsyncIterator<R>;
+    [Symbol.asyncIterator](options?: ReadableStreamIteratorOptions): ReadableStreamAsyncIterator<R>;
   }
 }
-
diff --git a/etc/web-streams-polyfill.api.md b/etc/web-streams-polyfill.api.md
index e269ec8..44563e1 100644
--- a/etc/web-streams-polyfill.api.md
+++ b/etc/web-streams-polyfill.api.md
@@ -52,8 +52,8 @@ export class ReadableByteStreamController {
 }
 
 // @public
-export class ReadableStream<R = any> {
-    [Symbol.asyncIterator]: (options?: ReadableStreamIteratorOptions) => ReadableStreamAsyncIterator<R>;
+export class ReadableStream<R = any> implements AsyncIterable<R> {
+    [Symbol.asyncIterator](options?: ReadableStreamIteratorOptions): ReadableStreamAsyncIterator<R>;
     constructor(underlyingSource: UnderlyingByteSource, strategy?: {
         highWaterMark?: number;
         size?: undefined;
@@ -76,7 +76,7 @@ export class ReadableStream<R = any> {
 }
 
 // @public
-export interface ReadableStreamAsyncIterator<R> extends AsyncIterator<R> {
+export interface ReadableStreamAsyncIterator<R> extends AsyncIterableIterator<R> {
     // (undocumented)
     next(): Promise<IteratorResult<R, undefined>>;
     // (undocumented)
diff --git a/rollup.config.mjs b/rollup.config.mjs
index 51139c2..4293de9 100644
--- a/rollup.config.mjs
+++ b/rollup.config.mjs
@@ -69,13 +69,13 @@ function bundle(entry, { esm = false, minify = false, target = 'es5' } = {}) {
         declaration: false,
         declarationMap: false
       }),
-      inject({
+      target === 'es5' ? inject({
         include: 'src/**/*.ts',
         exclude: 'src/stub/symbol.ts',
         modules: {
           Symbol: path.resolve(dirname, './src/stub/symbol.ts')
         }
-      }),
+      }) : undefined,
       replace({
         include: 'src/**/*.ts',
         preventAssignment: true,
diff --git a/src/lib/abstract-ops/ecmascript.ts b/src/lib/abstract-ops/ecmascript.ts
index 546fc19..6a07db4 100644
--- a/src/lib/abstract-ops/ecmascript.ts
+++ b/src/lib/abstract-ops/ecmascript.ts
@@ -110,6 +110,12 @@ export function CreateAsyncFromSyncIterator<T>(syncIteratorRecord: SyncIteratorR
   return { iterator: asyncIterator, nextMethod, done: false };
 }
 
+// Aligns with core-js/modules/es.symbol.async-iterator.js
+export const SymbolAsyncIterator: (typeof Symbol)['asyncIterator'] =
+  Symbol.asyncIterator ??
+  Symbol.for?.('Symbol.asyncIterator') ??
+  '@@asyncIterator';
+
 export type SyncOrAsyncIterable<T> = Iterable<T> | AsyncIterable<T>;
 export type SyncOrAsyncIteratorMethod<T> = () => (Iterator<T> | AsyncIterator<T>);
 
@@ -131,7 +137,7 @@ function GetIterator<T>(
   assert(hint === 'sync' || hint === 'async');
   if (method === undefined) {
     if (hint === 'async') {
-      method = GetMethod(obj as AsyncIterable<T>, Symbol.asyncIterator);
+      method = GetMethod(obj as AsyncIterable<T>, SymbolAsyncIterator);
       if (method === undefined) {
         const syncMethod = GetMethod(obj as Iterable<T>, Symbol.iterator);
         const syncIteratorRecord = GetIterator(obj as Iterable<T>, 'sync', syncMethod);
diff --git a/src/lib/readable-stream.ts b/src/lib/readable-stream.ts
index 74446a4..dbfbb58 100644
--- a/src/lib/readable-stream.ts
+++ b/src/lib/readable-stream.ts
@@ -49,7 +49,7 @@ import type {
 } from './readable-stream/underlying-source';
 import { noop } from '../utils';
 import { setFunctionName, typeIsObject } from './helpers/miscellaneous';
-import { CreateArrayFromList } from './abstract-ops/ecmascript';
+import { CreateArrayFromList, SymbolAsyncIterator } from './abstract-ops/ecmascript';
 import { CancelSteps } from './abstract-ops/internal-methods';
 import { IsNonNegativeNumber } from './abstract-ops/miscellaneous';
 import { assertObject, assertRequiredArgument } from './validators/basic';
@@ -85,7 +85,7 @@ type ReadableStreamState = 'readable' | 'closed' | 'errored';
  *
  * @public
  */
-export class ReadableStream<R = any> {
+export class ReadableStream<R = any> implements AsyncIterable<R> {
   /** @internal */
   _state!: ReadableStreamState;
   /** @internal */
@@ -329,7 +329,12 @@ export class ReadableStream<R = any> {
   /**
    * {@inheritDoc ReadableStream.values}
    */
-  [Symbol.asyncIterator]!: (options?: ReadableStreamIteratorOptions) => ReadableStreamAsyncIterator<R>;
+  [Symbol.asyncIterator](options?: ReadableStreamIteratorOptions): ReadableStreamAsyncIterator<R>;
+
+  [SymbolAsyncIterator](options?: ReadableStreamIteratorOptions): ReadableStreamAsyncIterator<R> {
+    // Stub implementation, overridden below
+    return this.values(options);
+  }
 
   /**
    * Creates a new ReadableStream wrapping the provided iterable or async iterable.
@@ -367,13 +372,11 @@ if (typeof Symbol.toStringTag === 'symbol') {
     configurable: true
   });
 }
-if (typeof Symbol.asyncIterator === 'symbol') {
-  Object.defineProperty(ReadableStream.prototype, Symbol.asyncIterator, {
-    value: ReadableStream.prototype.values,
-    writable: true,
-    configurable: true
-  });
-}
+Object.defineProperty(ReadableStream.prototype, SymbolAsyncIterator, {
+  value: ReadableStream.prototype.values,
+  writable: true,
+  configurable: true
+});
 
 export type {
   ReadableStreamAsyncIterator,
diff --git a/src/lib/readable-stream/async-iterator.ts b/src/lib/readable-stream/async-iterator.ts
index e504ded..b7abf8d 100644
--- a/src/lib/readable-stream/async-iterator.ts
+++ b/src/lib/readable-stream/async-iterator.ts
@@ -25,7 +25,7 @@ import {
  *
  * @public
  */
-export interface ReadableStreamAsyncIterator<R> extends AsyncIterator<R> {
+export interface ReadableStreamAsyncIterator<R> extends AsyncIterableIterator<R> {
   next(): Promise<IteratorResult<R, undefined>>;
 
   return(value?: any): Promise<IteratorResult<any>>;
@@ -140,9 +140,7 @@ const ReadableStreamAsyncIteratorPrototype: ReadableStreamAsyncIteratorInstance<
     return this._asyncIteratorImpl.return(value);
   }
 } as any;
-if (AsyncIteratorPrototype !== undefined) {
-  Object.setPrototypeOf(ReadableStreamAsyncIteratorPrototype, AsyncIteratorPrototype);
-}
+Object.setPrototypeOf(ReadableStreamAsyncIteratorPrototype, AsyncIteratorPrototype);
 
 // Abstract operations for the ReadableStream.
 
diff --git a/src/target/es2018/stub/async-iterator-prototype.ts b/src/target/es2018/stub/async-iterator-prototype.ts
index c8f11d8..b25f071 100644
--- a/src/target/es2018/stub/async-iterator-prototype.ts
+++ b/src/target/es2018/stub/async-iterator-prototype.ts
@@ -1,5 +1,5 @@
 /// <reference lib="es2018.asynciterable" />
 
 /* eslint-disable @typescript-eslint/no-empty-function */
-export const AsyncIteratorPrototype: AsyncIterable<any> | undefined =
+export const AsyncIteratorPrototype: AsyncIterable<any> =
   Object.getPrototypeOf(Object.getPrototypeOf(async function* (): AsyncIterableIterator<any> {}).prototype);
diff --git a/src/target/es5/stub/async-iterator-prototype.ts b/src/target/es5/stub/async-iterator-prototype.ts
index 4839a2b..6455bd3 100644
--- a/src/target/es5/stub/async-iterator-prototype.ts
+++ b/src/target/es5/stub/async-iterator-prototype.ts
@@ -1,16 +1,13 @@
 /// <reference lib="es2018.asynciterable" />
 
-export let AsyncIteratorPrototype: AsyncIterable<any> | undefined;
+import { SymbolAsyncIterator } from '../../../lib/abstract-ops/ecmascript';
 
-if (typeof Symbol.asyncIterator === 'symbol') {
-  // We're running inside a ES2018+ environment, but we're compiling to an older syntax.
-  // We cannot access %AsyncIteratorPrototype% without non-ES2018 syntax, but we can re-create it.
-  AsyncIteratorPrototype = {
-    // 25.1.3.1 %AsyncIteratorPrototype% [ @@asyncIterator ] ( )
-    // https://tc39.github.io/ecma262/#sec-asynciteratorprototype-asynciterator
-    [Symbol.asyncIterator](this: AsyncIterator<any>) {
-      return this;
-    }
-  };
-  Object.defineProperty(AsyncIteratorPrototype, Symbol.asyncIterator, { enumerable: false });
-}
+// We cannot access %AsyncIteratorPrototype% without non-ES2018 syntax, but we can re-create it.
+export const AsyncIteratorPrototype: AsyncIterable<any> = {
+  // 25.1.3.1 %AsyncIteratorPrototype% [ @@asyncIterator ] ( )
+  // https://tc39.github.io/ecma262/#sec-asynciteratorprototype-asynciterator
+  [SymbolAsyncIterator](this: AsyncIterator<any>) {
+    return this;
+  }
+};
+Object.defineProperty(AsyncIteratorPrototype, SymbolAsyncIterator, { enumerable: false });