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Issues and things to do to make this proj work


Data Handling

Tiff file loading

  • it will only really work for micromanager files, will need tweaks for other tiffs

Efficient storage and processing

  • memmap handles this well, but processing is slow
  • Start using Dask to speed up processing with multithreading

Segmentation

  • Fix Display issues, brightness fluctuates in openCV, but not in imageJ
    • This could be due to some averaging in the x,y,z resolutions?
    • this could also be due to normalization or data conversion issues!
  • Stardist segmentation is useless at the moment
    • again, due to normalization or datatype problems is likely
    • this could also be due to the fact that the brightness fluctuates so much in python vs imageJ

Tracking

  • use graph based method to register the same cells over long time periods