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Improve representation of (alignment)score in primersearch output BEDfile #80

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florianzwagemaker opened this issue Sep 23, 2024 · 0 comments
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@florianzwagemaker
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The score in the output BEDfile from the primersearch process currently represents a 'raw' score of the nuc44 scoring matrix combined with custom optimized gopen/gextend values.
This results in the score not being all that useful for the primersearch process as it becomes unreasonably hard to quickly understand if a found primer aligned well or not based on a score of 95, for example. Especially because we're aligning, and scoring, very short sequences.

It's probably better to represent this in another metric, like a percentage or edit distance.
i.e. when using a percentage, if the primer has a length of 30bp and the 'score' is 90. Then it's easy to determine that at least 27 of the 30 primer nucleotides were a match.

@florianzwagemaker florianzwagemaker added the enhancement New feature or request label Sep 23, 2024
@florianzwagemaker florianzwagemaker added this to the 2.0.0 milestone Sep 23, 2024
@florianzwagemaker florianzwagemaker changed the title Improve represenation of (alignment)score in primersearch output BEDfile Improve representation of (alignment)score in primersearch output BEDfile Oct 22, 2024
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