Affinity maturation advice #319
wisdomjack
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I’ve been using a computational pipeline involving RFdiffusion, ProteinMPNN, and AlphaFold to design protein binders targeting a specific protein of interest. After generating potential binders and assessing them, I’ve identified several hits that show promise.
Could you provide advice or recommend strategies on how to further optimize these binders? Specifically, I’m looking for ways to enhance their binding affinity? Like should I do a computational Alanine scan with rosetta followed by proteinMPNN or something similar?
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