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Nucleosome sequencing analysis for Loxodes magnus

Loxodes magnus MIC and MAC nuclei were sorted with FACS. Nucleosomal DNA was prepared with Atlantis dsDNAse digest.

Workflow outline

  • Map nucleosomal DNA library to Loxodes magnus MAC reference assembly (Falcon assembler)
  • Also filter assembly to exclude low-complexity repeat regions previously annotated with TRF (min 1 kbp region)
  • Evaluate coverage and nucleosomal DNA fragment length distribution
  • Global phaseogram of nucleosome positioning relative to each other
  • Phaseogram of nucleosome positioning relative to starts of gene features (TSS data not available)