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01_Cellranger installation on ERISOne.md

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To install Cellranger on ERISOne

Prerequisites:

  • Open an ERISOne account.
  • If on BWH campus, internet should under Wifi: phswifi3.
  • If WFH, set up VPN to connect to ERISOne. VPN set up see tutorial "BWH VPN set up".

Once log onto ERISOne, follow the following codes.

cd
mkdir yard
cd yard
mkdir apps
cd apps
curl -o cellranger-6.0.0.tar.gz "https://cf.10xgenomics.com/releases/cell-exp/cellranger-6.0.0.tar.gz?Expires=1616375849&Policy=eyJTdGF0ZW1lbnQiOlt7IlJlc291cmNlIjoiaHR0cHM6Ly9jZi4xMHhnZW5vbWljcy5jb20vcmVsZWFzZXMvY2VsbC1leHAvY2VsbHJhbmdlci02LjAuMC50YXIuZ3oiLCJDb25kaXRpb24iOnsiRGF0ZUxlc3NUaGFuIjp7IkFXUzpFcG9jaFRpbWUiOjE2MTYzNzU4NDl9fX1dfQ__&Signature=XlsdSdwGmuKJZZm1CudfNfVUF0hBJ7AnG63UK4I1rZQMdIem9ANlXvcuozJYGi1R9JMHEO8siNpqVHlUj9u9gyLx~DI4~XEYpheH6offBuY14jd7IFl4za1rXj6FG1eTLXHzDSe8bllLAgWmFp7DkZ4Nk0oq6OaFboDCOj150Iey7-~rOy07LfLljlgUD1SkFZRjBgZqkYz4L5WEeTZ-Kk7ZB6s~PSC75LyuX5gfHu8cyHj8uUBnXHuhZPrTmoyry4X7f5XRWjFdJeml62mRt2VhcX5bA7pA9rtkRxsywj7UBEUcGNt-VYhDiBbtbndFZCcU3jYpTxklgkwJTG2xJQ__&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA"
tar -zxvf cellranger-6.0.0.tar.gz
cd cellranger-6.0.0
cd /PHShome/tz949/yard/apps/cellranger-6.0.0
export PATH=/PHShome/tz949/yard/apps/cellranger-6.0.0:$PATH
which cellranger

After each log off and log in, cellranger needs to be added to the PATH as follows:

export PATH=/PHShome/tz949/yard/apps/cellranger-6.0.0:$PATH