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bowtie2_PE.cwl
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bowtie2_PE.cwl
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#!/usr/bin/env cwl-runner
cwlVersion: v1.0
class: CommandLineTool
label: "bowtie2 execution script: Paired-Ends mode"
baseCommand: bowtie2
hints:
SoftwareRequirement:
packages:
bowtie2:
specs: [ "http://bowtie-bio.sourceforge.net/bowtie2/index.shtml" ]
version: [ "2.3.4.1" ]
inputs:
fastq_flag:
type: boolean
inputBinding:
position: 1
prefix: -q
seed_n:
type: int
inputBinding:
position: 2
separate: false
prefix: -N
index_loc:
type: Any
inputBinding:
position: 4
prefix: -x
separate: true
match_n:
type: int
inputBinding:
prefix: -k
separate: false
position: 3
input_PE_R1_fastq:
type: File
inputBinding:
prefix: "-1"
separate: true
itemSeparator: ","
position: 5
input_PE_R2_fastq:
type: File
inputBinding:
prefix: "-2"
separate: true
itemSeparator: ","
position: 6
output_sam:
type: string
inputBinding:
prefix: -S
separate: true
position: 8
processors:
type: int
inputBinding:
prefix: -p
separate: true
position: 7
local_alignment:
type: boolean
inputBinding:
position: 9
prefix: --local
unaligned_items:
type: boolean
inputBinding:
position: 10
prefix: --no-unal
outputs:
output_file_sam:
type: File
outputBinding:
glob: $(inputs.output_sam)