diff --git a/tools/interactive/interactivetool_cellxgene_1.1.1.xml b/tools/interactive/interactivetool_cellxgene_1.1.1.xml
index 08edd9273b4d..c8c6c4cb72bc 100644
--- a/tools/interactive/interactivetool_cellxgene_1.1.1.xml
+++ b/tools/interactive/interactivetool_cellxgene_1.1.1.xml
@@ -15,20 +15,23 @@
 			   />
     </stdio>
     <command><![CDATA[
-        #import re
-        #set $fancy_name = '/tmp/galaxy_cellxgene_' + re.sub('[^\w\-_]', '_', $infile.element_identifier) + '.h5ad'
-	
-	#if $var_name
-	python '${anndata_unique_var_gene_symbols}' '${infile}' '${fancy_name}' '${var_name}'
-        #else  
-        cp '${infile}' '${fancy_name}'
-        #end if
-	&&
-	mkdir annotation_files
-	&&
-	cellxgene launch --host 0.0.0.0 --port 80 
+    #import re
+    #set $fancy_name = '/tmp/galaxy_cellxgene_' + re.sub('[^\w\-_]', '_', $infile.element_identifier) + '.h5ad'
+
+    #if ($var_name and $make_unique) or $layer:
+    python '${anndata_unique_var_gene_symbols}' '${infile}' '${fancy_name}' '${var_name}' '${make_unique}' '${layer}'
+    #else  
+    cp '${infile}' '${fancy_name}'
+    #end if
+    &&
+    mkdir annotation_files
+    &&
+    cellxgene launch --host 0.0.0.0 --port 80 
         --user-generated-data-dir annotation_files
-	'${fancy_name}'
+    #if $var_name and not $make_unique:
+        --var-names $var_name
+    #end if
+    '${fancy_name}'
     ]]>
     </command>
     <configfiles>
@@ -44,21 +47,31 @@ def rn(df, field, suffix = '-duplicate-'):
 adata = ad.read_h5ad(sys.argv[1])
 output = sys.argv[2]
 gene_symbol_field = sys.argv[3]
-if gene_symbol_field not in adata.var.keys():
-   sys.exit(f"Field {gene_symbol_field} set as var_name does not exist in the var object. AnnData object will be used as it was given")
-
-adata.var = rn(adata.var, gene_symbol_field, suffix = "_d")
+make_unique = (sys.argv[4].lower() == "true")
+layer = sys.argv[5]
 
-adata.var["extra_gene_id"] = adata.var.index
-adata.var = adata.var.set_index(f"{gene_symbol_field}_u")
+if gene_symbol_field and make_unique:
+    if gene_symbol_field not in adata.var.keys():
+        sys.exit(f"Field {gene_symbol_field} set as var_name does not exist in the var object. AnnData object will be used as it was given")
+    
+    adata.var = rn(adata.var, gene_symbol_field, suffix = "_d")
+    adata.var["extra_gene_id"] = adata.var.index
+    adata.var = adata.var.set_index(f"{gene_symbol_field}_u")
 
+if layer:
+    if layer not in adata.layers.keys():
+        sys.exit(f"Layer {layer} is not present in AnnData, only available layers are: {', '.join(adata.layers.keys())}")
+    else:
+        adata.X = adata.layers[layer]
 
-adata.write_h5ad(output, compression="gzip")
+adata.write_h5ad(output)
 ]]></configfile>
     </configfiles>
     <inputs>
 	<param name="infile" type="data" format="h5ad" label="Concatenate Dataset"/>
-        <param name="var_name" type="text" optional="true" label="Var field for gene symbols" help="Optionaly specify the var field from the AnnData file provided where gene symbols are available. Usually the AnnData file var slot will be indexed with gene symbols and this is not required. Using this option will delay the startup of the cellxgene tool, as the AnnData needs to be modified and re-written to disk."/>
+    <param name="var_name" type="text" optional="true" label="Var field for gene symbols" help="Optionaly specify the var field from the AnnData file provided where gene symbols are available. Usually the AnnData file var slot will be indexed with gene symbols and this is not required. Using this option will delay the startup of the cellxgene tool, as the AnnData needs to be modified and re-written to disk."/>
+    <param name="make_unique" type="boolean" checked="false" label="Make specified var field unique" help="It will copy the specified var field above and make its values unique. This means reading the AnnData object into Python and re-writing it again, increasing waiting time for the container to be up."/>
+    <param name="layer" type="text" optional="true" label="Specifies the AnnData layer to use as matrix" help="The layer name needs to be present in the AnnData file or this tool will fail"/>
     </inputs>
     <outputs>
 	<data name="out_file1" format="txt" />
@@ -80,9 +93,20 @@ Selecting the Var name
 ----------------------
 It can happen that the main index for the var element of AnnData is not the 
 gene symbol field, in which case search by genes will probably be by identifier.
-You can choose a different field (which contains the gene symbols) so that
+You can choose a different field and celxgene will use this. If in addition you choose "Make unique", 
 the AnnData is modified (in a new copy) so that that field is made unique and
-it is indexed by it. Then cellxgene will allow searches by genes in that field.
+it is indexed by it. Making it unique entails though loading the object into memory, 
+modifying and writing it back, which can delay the execution of cellxgene.
+
+Then cellxgene will allow searches by genes in that field.
+
+Selecting the layer
+-------------------
+
+It can happen that the AnnData object contains multiple layers, for example
+one with the raw counts and another with the normalised counts. You can select
+which layer to use as the matrix for cellxgene. By default cellxgene will use the X slot, but
+that slot might not contain the matrix that you want to visualise.
 
 Outputs
 -------
diff --git a/tools/interactive/interactivetool_guacamole_desktop.xml b/tools/interactive/interactivetool_guacamole_desktop.xml
index 665f4d33eb81..dacf1589b276 100644
--- a/tools/interactive/interactivetool_guacamole_desktop.xml
+++ b/tools/interactive/interactivetool_guacamole_desktop.xml
@@ -3,7 +3,7 @@
         <container type="docker">quay.io/bgruening/guacamole-desktop</container>
     </requirements>
     <entry_points>
-        <entry_point name="Remote Desktop" requires_domain="True">
+        <entry_point name="Remote Desktop" requires_domain="False" requires_path_in_url="False">
             <port>8080</port>
             <url><![CDATA[?username=user&password=password]]></url>
         </entry_point>
diff --git a/tools/interactive/interactivetool_jupyter_notebook_1.0.0.xml b/tools/interactive/interactivetool_jupyter_notebook_1.0.0.xml
index c7e4c9bb8508..5b5e91e16144 100644
--- a/tools/interactive/interactivetool_jupyter_notebook_1.0.0.xml
+++ b/tools/interactive/interactivetool_jupyter_notebook_1.0.0.xml
@@ -1,9 +1,9 @@
-<tool id="interactive_tool_jupyter_notebook" tool_type="interactive" name="Interactive JupyTool and notebook" version="1.0.0" profile="22.01">
+<tool id="interactive_tool_jupyter_notebook" tool_type="interactive" name="Interactive JupyterLab Notebook" version="1.0.0" profile="22.01">
     <requirements>
         <container type="docker">quay.io/bgruening/docker-jupyter-notebook:2021-03-05</container>
     </requirements>
     <entry_points>
-        <entry_point name="JupyTool interactive tool" requires_domain="True">
+        <entry_point name="JupyTool interactive tool" label="jupytool" requires_domain="False" requires_path_in_url="True">
             <port>8888</port>
             <url>ipython/lab</url>
         </entry_point>
@@ -14,6 +14,7 @@
         <environment_variable name="GALAXY_WEB_PORT">8080</environment_variable>
         <environment_variable name="GALAXY_URL">$__galaxy_url__</environment_variable>
         <environment_variable name="API_KEY" inject="api_key"/>
+        <environment_variable name="EP_PATH" inject="entry_point_path_for_label">jupytool</environment_variable>
     </environment_variables>
     <configfiles>
         <inputs name="inputs" filename="galaxy_inputs.json" data_style="staging_path_and_source_path"/>
@@ -80,6 +81,7 @@ except FileNotFoundError:
         export GALAXY_WORKING_DIR=`pwd` &&
         mkdir -p ./jupyter/outputs/collection &&
         mkdir -p ./jupyter/galaxy_inputs &&
+        export PROXY_PREFIX=\${EP_PATH%/ipython*} &&
 
         ## change into the directory where the notebooks are located
         cd ./jupyter/ &&
diff --git a/tools/interactive/interactivetool_mgnify_notebook.xml b/tools/interactive/interactivetool_mgnify_notebook.xml
index ea26f7c25f3f..5b2f93e17cfe 100644
--- a/tools/interactive/interactivetool_mgnify_notebook.xml
+++ b/tools/interactive/interactivetool_mgnify_notebook.xml
@@ -1,6 +1,6 @@
-<tool id="interactive_tool_mgnify_notebook" tool_type="interactive" name="Interactive MGnify Notebook" version="0.0.1" profile="22.01">
+<tool id="interactive_tool_mgnify_notebook" tool_type="interactive" name="Interactive MGnify Notebook" version="1.2.2" profile="22.01">
     <requirements>
-        <container type="docker">quay.io/microbiome-informatics/emg-notebooks.dev:v1.0.1</container>
+        <container type="docker">quay.io/microbiome-informatics/emg-notebooks.dev:v1.2.2</container>
     </requirements>
     <entry_points>
         <entry_point name="MGnify Interactive Tool" requires_domain="True">
diff --git a/tools/interactive/interactivetool_openrefine.xml b/tools/interactive/interactivetool_openrefine.xml
index e7dde3d47eed..1c8d9d96fb89 100644
--- a/tools/interactive/interactivetool_openrefine.xml
+++ b/tools/interactive/interactivetool_openrefine.xml
@@ -4,7 +4,7 @@
         <container type="docker">ylebras/openrefine-docker</container>
     </requirements>
     <entry_points>
-        <entry_point name="Openrefine visualisation" requires_domain="True">
+        <entry_point name="Openrefine visualisation" requires_domain="False" requires_path_in_url="False">
             <port>3333</port>
         </entry_point>
     </entry_points>
@@ -17,7 +17,7 @@
     </environment_variables>
     <configfiles>
         <configfile name="start_openrefine"><![CDATA[
-exec /OpenRefine/refine -i 0.0.0.0 -m \${GALAXY_MEMORY_MB}M &
+exec /OpenRefine/refine -i 0.0.0.0 -m \$GALAXY_MEMORY_MB &
 
 ##Check if openrefine is up to work
 STATUS=\$(curl --include 'http://127.0.0.1:3333' 2>&1)
@@ -81,4 +81,3 @@ Example input file (TAB separated)::
 ]]>
     </help>
 </tool>
-
diff --git a/tools/interactive/interactivetool_pangeo_notebook.xml b/tools/interactive/interactivetool_pangeo_notebook.xml
index 831a224514fd..b3c521d1c8cb 100644
--- a/tools/interactive/interactivetool_pangeo_notebook.xml
+++ b/tools/interactive/interactivetool_pangeo_notebook.xml
@@ -1,6 +1,6 @@
 <tool id="interactive_tool_pangeo_notebook" tool_type="interactive" name="Interactive Pangeo Notebook" version="22.01" profile="22.01">
     <requirements>
-        <container type="docker">quay.io/nordicesmhub/docker-pangeo-notebook:2023.02.27</container>
+        <container type="docker">quay.io/nordicesmhub/docker-pangeo-notebook:1c0f66b</container>
     </requirements>
     <entry_points>
         <entry_point name="Pangeo Interactive Tool" requires_domain="True">
diff --git a/tools/interactive/interactivetool_rstudio.xml b/tools/interactive/interactivetool_rstudio.xml
index df344e444232..ee987d7c2c2f 100644
--- a/tools/interactive/interactivetool_rstudio.xml
+++ b/tools/interactive/interactivetool_rstudio.xml
@@ -3,14 +3,13 @@
         <container type="docker">quay.io/galaxy/docker-rstudio-notebook:23.1</container>
     </requirements>
     <entry_points>
-        <entry_point name="RStudio" requires_domain="True">
+        <entry_point name="RStudio" requires_domain="False" requires_path_in_header_named="X-RStudio-Root-Path">
             <port>8787</port>
             <url>/</url>
         </entry_point>
     </entry_points>
     <environment_variables>
         <environment_variable name="HISTORY_ID" strip="True">${__app__.security.encode_id($jupyter_notebook.history_id)}</environment_variable> <!-- FIXME: Warning: The use of __app__ is deprecated and will break backward compatibility in the near future -->
-        <environment_variable name="REMOTE_HOST">${__app__.config.galaxy_infrastructure_url}</environment_variable> <!-- FIXME: Warning: The use of __app__ is deprecated and will break backward compatibility in the near future -->
         <environment_variable name="GALAXY_WEB_PORT">8080</environment_variable>
         <environment_variable name="GALAXY_URL">$__galaxy_url__</environment_variable>
         <environment_variable name="DEBUG">true</environment_variable>