diff --git a/browser/ui/color/brave_color_id.h b/browser/ui/color/brave_color_id.h
index 32c363832532..1d6555f3037b 100644
--- a/browser/ui/color/brave_color_id.h
+++ b/browser/ui/color/brave_color_id.h
@@ -112,6 +112,7 @@
 #define BRAVE_VERTICAL_TAB_COLOR_IDS                        \
     E_CPONLY(kColorBraveVerticalTabSeparator)               \
     E_CPONLY(kColorBraveVerticalTabActiveBackground)        \
+    E_CPONLY(kColorBraveVerticalTabHoveredBackground)       \
     E_CPONLY(kColorBraveVerticalTabInactiveBackground)      \
     E_CPONLY(kColorBraveVerticalTabNTBIconColor)            \
     E_CPONLY(kColorBraveVerticalTabNTBTextColor)            \
diff --git a/browser/ui/tabs/brave_tab_color_mixer.cc b/browser/ui/tabs/brave_tab_color_mixer.cc
index 120a3cb9f16d..f2877fd6596e 100644
--- a/browser/ui/tabs/brave_tab_color_mixer.cc
+++ b/browser/ui/tabs/brave_tab_color_mixer.cc
@@ -22,7 +22,9 @@ void AddBraveTabThemeColorMixer(ui::ColorProvider* provider,
   auto& mixer = provider->AddMixer();
 
   mixer[kColorBraveVerticalTabActiveBackground] = {
-      kColorTabBackgroundInactiveFrameActive};
+      nala::kColorDesktopbrowserTabbarActiveTabVertical};
+  mixer[kColorBraveVerticalTabHoveredBackground] = {
+      nala::kColorDesktopbrowserTabbarHoverTabVertical};
   mixer[kColorBraveVerticalTabInactiveBackground] = {kColorToolbar};
   mixer[kColorBraveVerticalTabSeparator] = {kColorToolbarContentAreaSeparator};
   mixer[kColorBraveVerticalTabNTBIconColor] = {
@@ -61,13 +63,17 @@ void AddBraveTabPrivateThemeColorMixer(ui::ColorProvider* provider,
                                        const ui::ColorProviderKey& key) {
   auto& mixer = provider->AddMixer();
   mixer[kColorBraveVerticalTabActiveBackground] = {
-      mixer.GetResultColor(kColorTabBackgroundActiveFrameActive)};
+      nala::kColorPrimitivePrivateWindow30};
   mixer[kColorBraveVerticalTabInactiveBackground] = {
       mixer.GetResultColor(kColorToolbar)};
+  mixer[kColorBraveVerticalTabHoveredBackground] = {
+      ui::AlphaBlend(kColorBraveVerticalTabActiveBackground,
+                     kColorBraveVerticalTabInactiveBackground,
+                     /* 40% opacity */ 0.4 * SK_AlphaOPAQUE)};
   mixer[kColorBraveSplitViewTileBackgroundHorizontal] = {
       nala::kColorPrimitivePrivateWindow10};
   mixer[kColorBraveSplitViewTileBackgroundVertical] = {
-      kColorBraveSplitViewTileBackgroundHorizontal};
+      nala::kColorPrimitivePrivateWindow5};
   mixer[kColorBraveSplitViewTileDivider] = {
       nala::kColorPrimitivePrivateWindow20};
 }
@@ -76,13 +82,17 @@ void AddBraveTabTorThemeColorMixer(ui::ColorProvider* provider,
                                    const ui::ColorProviderKey& key) {
   auto& mixer = provider->AddMixer();
   mixer[kColorBraveVerticalTabActiveBackground] = {
-      mixer.GetResultColor(kColorTabBackgroundActiveFrameActive)};
+      nala::kColorPrimitiveTorWindow30};
   mixer[kColorBraveVerticalTabInactiveBackground] = {
       mixer.GetResultColor(kColorToolbar)};
+  mixer[kColorBraveVerticalTabHoveredBackground] = {
+      ui::AlphaBlend(kColorBraveVerticalTabActiveBackground,
+                     kColorBraveVerticalTabInactiveBackground,
+                     /* 40% opacity */ 0.4 * SK_AlphaOPAQUE)};
   mixer[kColorBraveSplitViewTileBackgroundHorizontal] = {
       nala::kColorPrimitiveTorWindow10};
   mixer[kColorBraveSplitViewTileBackgroundVertical] = {
-      kColorBraveSplitViewTileBackgroundHorizontal};
+      nala::kColorPrimitiveTorWindow5};
   mixer[kColorBraveSplitViewTileDivider] = {nala::kColorPrimitiveTorWindow20};
 }
 
diff --git a/browser/ui/views/tabs/brave_tab_style_views.inc.cc b/browser/ui/views/tabs/brave_tab_style_views.inc.cc
index 8ac87495e769..f207aa604bd0 100644
--- a/browser/ui/views/tabs/brave_tab_style_views.inc.cc
+++ b/browser/ui/views/tabs/brave_tab_style_views.inc.cc
@@ -445,11 +445,11 @@ SkColor BraveVerticalTabStyle::GetTargetTabBackgroundColor(
   }
 
   if (tab()->IsActive()) {
-    return cp->GetColor(nala::kColorDesktopbrowserTabbarActiveTabVertical);
+    return cp->GetColor(kColorBraveVerticalTabActiveBackground);
   }
 
   if (hovered) {
-    return cp->GetColor(nala::kColorDesktopbrowserTabbarHoverTabVertical);
+    return cp->GetColor(kColorBraveVerticalTabHoveredBackground);
   }
 
   if (selection_state == TabStyle::TabSelectionState::kSelected) {