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When I use gatk haplotypecaller to get vcf from WES bam file, it stopped at somewhere of chr4. The error information is "java.lang.IllegalStateException: Never found start 0 or stop -1 given cigar 23M90D98M". Counld you help me to debug it?
My gatk version is 4.1.6.0.
All error information is as bellows:
14:47:36.134 INFO ProgressMeter - chr3:195451651 47.5 141130 2974.2
14:47:46.204 INFO ProgressMeter - chr3:195937445 47.6 141450 2970.4
14:47:56.209 INFO ProgressMeter - chr3:197401760 47.8 142070 2973.0
14:48:06.317 INFO ProgressMeter - chr4:944918 48.0 142740 2976.5
14:48:12.491 INFO VectorLoglessPairHMM - Time spent in setup for JNI call : 1.403408197
14:48:12.491 INFO PairHMM - Total compute time in PairHMM computeLogLikelihoods() : 110.672423397
14:48:12.491 INFO SmithWatermanAligner - Total compute time in java Smith-Waterman : 120.18 sec
14:48:12.492 INFO HaplotypeCaller - Shutting down engine
[September 13, 2024 at 2:48:12 PM CST] org.broadinstitute.hellbender.tools.walkers.haplotypecaller.HaplotypeCaller done. Elapsed time: 48.27 minutes.
Runtime.totalMemory()=708837376
java.lang.IllegalStateException: Never found start 0 or stop -1 given cigar 23M90D98M
at org.broadinstitute.hellbender.utils.read.AlignmentUtils.getBasesCoveringRefInterval(AlignmentUtils.java:204)
at org.broadinstitute.hellbender.utils.haplotype.Haplotype.trim(Haplotype.java:95)
at org.broadinstitute.hellbender.tools.walkers.haplotypecaller.AssemblyResultSet.trimDownHaplotypes(AssemblyResultSet.java:135)
at org.broadinstitute.hellbender.tools.walkers.haplotypecaller.AssemblyResultSet.calculateOriginalByTrimmedHaplotypes(AssemblyResultSet.java:111)
at org.broadinstitute.hellbender.tools.walkers.haplotypecaller.AssemblyResultSet.trimTo(AssemblyResultSet.java:73)
at org.broadinstitute.hellbender.tools.walkers.haplotypecaller.HaplotypeCallerEngine.callRegion(HaplotypeCallerEngine.java:558)
at org.broadinstitute.hellbender.tools.walkers.haplotypecaller.HaplotypeCaller.apply(HaplotypeCaller.java:212)
at org.broadinstitute.hellbender.engine.AssemblyRegionWalker.processReadShard(AssemblyRegionWalker.java:200)
at org.broadinstitute.hellbender.engine.AssemblyRegionWalker.traverse(AssemblyRegionWalker.java:173)
at org.broadinstitute.hellbender.engine.GATKTool.doWork(GATKTool.java:1048)
at org.broadinstitute.hellbender.cmdline.CommandLineProgram.runTool(CommandLineProgram.java:139)
at org.broadinstitute.hellbender.cmdline.CommandLineProgram.instanceMainPostParseArgs(CommandLineProgram.java:191)
at org.broadinstitute.hellbender.cmdline.CommandLineProgram.instanceMain(CommandLineProgram.java:210)
at org.broadinstitute.hellbender.Main.runCommandLineProgram(Main.java:163)
at org.broadinstitute.hellbender.Main.mainEntry(Main.java:206)
at org.broadinstitute.hellbender.Main.main(Main.java:292)
Using GATK jar /mnt/h/linuxdir/software/gatk/build/libs/gatk-package-4.1.6.0-SNAPSHOT-local.jar
Running:
java -Dsamjdk.use_async_io_read_samtools=false -Dsamjdk.use_async_io_write_samtools=true -Dsamjdk.use_async_io_write_tribble=false -Dsamjdk.compression_level=2 -jar /mnt/h/linuxdir/software/gatk/build/libs/gatk-package-4.1.6.0-SNAPSHOT-local.jar HaplotypeCaller -R /mnt/h/linuxdir/database/genome/human/refseq/hg19.fa -I /mnt/g/XY10023566/bwa/XY10023566.sorted.markdup.BQSR.bam -O /mnt/g/XY10023566/gatk/XY10023566.HaplotypeCaller.vcf.gz -L /mnt/h/linuxdir/database/genome/human/S07604624_hs_hg19/S07604624_Covered.bed -L /mnt/h/linuxdir/database/genome/human/grch37_exon_chrM.bed --alleles /mnt/h/linuxdir/wespipeline2.0/interestedsite/interested.sort.merged.vcf.gz
The text was updated successfully, but these errors were encountered:
@polymerase2010 I see that you're running GATK 4.1.6.0, which is almost 5 years old. Can you try the latest release (4.6.0.0), and see if the error goes away? There have been many fixes and improvements to HaplotypeCaller in the past 5 years.
When I use gatk haplotypecaller to get vcf from WES bam file, it stopped at somewhere of chr4. The error information is "java.lang.IllegalStateException: Never found start 0 or stop -1 given cigar 23M90D98M". Counld you help me to debug it?
My gatk version is 4.1.6.0.
All error information is as bellows:
14:47:36.134 INFO ProgressMeter - chr3:195451651 47.5 141130 2974.2
14:47:46.204 INFO ProgressMeter - chr3:195937445 47.6 141450 2970.4
14:47:56.209 INFO ProgressMeter - chr3:197401760 47.8 142070 2973.0
14:48:06.317 INFO ProgressMeter - chr4:944918 48.0 142740 2976.5
14:48:12.491 INFO VectorLoglessPairHMM - Time spent in setup for JNI call : 1.403408197
14:48:12.491 INFO PairHMM - Total compute time in PairHMM computeLogLikelihoods() : 110.672423397
14:48:12.491 INFO SmithWatermanAligner - Total compute time in java Smith-Waterman : 120.18 sec
14:48:12.492 INFO HaplotypeCaller - Shutting down engine
[September 13, 2024 at 2:48:12 PM CST] org.broadinstitute.hellbender.tools.walkers.haplotypecaller.HaplotypeCaller done. Elapsed time: 48.27 minutes.
Runtime.totalMemory()=708837376
java.lang.IllegalStateException: Never found start 0 or stop -1 given cigar 23M90D98M
at org.broadinstitute.hellbender.utils.read.AlignmentUtils.getBasesCoveringRefInterval(AlignmentUtils.java:204)
at org.broadinstitute.hellbender.utils.haplotype.Haplotype.trim(Haplotype.java:95)
at org.broadinstitute.hellbender.tools.walkers.haplotypecaller.AssemblyResultSet.trimDownHaplotypes(AssemblyResultSet.java:135)
at org.broadinstitute.hellbender.tools.walkers.haplotypecaller.AssemblyResultSet.calculateOriginalByTrimmedHaplotypes(AssemblyResultSet.java:111)
at org.broadinstitute.hellbender.tools.walkers.haplotypecaller.AssemblyResultSet.trimTo(AssemblyResultSet.java:73)
at org.broadinstitute.hellbender.tools.walkers.haplotypecaller.HaplotypeCallerEngine.callRegion(HaplotypeCallerEngine.java:558)
at org.broadinstitute.hellbender.tools.walkers.haplotypecaller.HaplotypeCaller.apply(HaplotypeCaller.java:212)
at org.broadinstitute.hellbender.engine.AssemblyRegionWalker.processReadShard(AssemblyRegionWalker.java:200)
at org.broadinstitute.hellbender.engine.AssemblyRegionWalker.traverse(AssemblyRegionWalker.java:173)
at org.broadinstitute.hellbender.engine.GATKTool.doWork(GATKTool.java:1048)
at org.broadinstitute.hellbender.cmdline.CommandLineProgram.runTool(CommandLineProgram.java:139)
at org.broadinstitute.hellbender.cmdline.CommandLineProgram.instanceMainPostParseArgs(CommandLineProgram.java:191)
at org.broadinstitute.hellbender.cmdline.CommandLineProgram.instanceMain(CommandLineProgram.java:210)
at org.broadinstitute.hellbender.Main.runCommandLineProgram(Main.java:163)
at org.broadinstitute.hellbender.Main.mainEntry(Main.java:206)
at org.broadinstitute.hellbender.Main.main(Main.java:292)
Using GATK jar /mnt/h/linuxdir/software/gatk/build/libs/gatk-package-4.1.6.0-SNAPSHOT-local.jar
Running:
java -Dsamjdk.use_async_io_read_samtools=false -Dsamjdk.use_async_io_write_samtools=true -Dsamjdk.use_async_io_write_tribble=false -Dsamjdk.compression_level=2 -jar /mnt/h/linuxdir/software/gatk/build/libs/gatk-package-4.1.6.0-SNAPSHOT-local.jar HaplotypeCaller -R /mnt/h/linuxdir/database/genome/human/refseq/hg19.fa -I /mnt/g/XY10023566/bwa/XY10023566.sorted.markdup.BQSR.bam -O /mnt/g/XY10023566/gatk/XY10023566.HaplotypeCaller.vcf.gz -L /mnt/h/linuxdir/database/genome/human/S07604624_hs_hg19/S07604624_Covered.bed -L /mnt/h/linuxdir/database/genome/human/grch37_exon_chrM.bed --alleles /mnt/h/linuxdir/wespipeline2.0/interestedsite/interested.sort.merged.vcf.gz
The text was updated successfully, but these errors were encountered: