This directory contains the raw imaging and sequencing data.
antagonists: in situ hybridization of Wnt antagonist genes.cloning: primer sequences and other information used for gene cloning.comparative: comparative analysis of gene expression variability.coverage: read coverage analysis for stage-specific transcriptome.domains: description of Terebratalia Wnt domains, including the divergent wnt1t.doubles: double fluorescent in situ hybridization of Wnt genes.ligands: colorimetric in situ hybridization of Wnt genes.morphology: stainings of Terebratalia transversa gastrula and larva.orthology: phylogenetic analyses of all Wnt signaling components.polarity: in situ hybridization of planar cell polarity genes.profiling: transcriptomic analyses of stage-specific RNA-seq data.receptors: in situ hybridization of Frizzled genes.sequences: transcript and protein sequences used in the study.
Most images were taken on a Zeiss Axioscope compound microscope using a 40x objective. The pixel resolution is not saved in the original files. To set the proper scale, you need to run a small ImageJ macro:
run("Set Scale...", "distance=7.8140 known=1 pixel=1 unit=micron");
run("Scale Bar...", "width=20 height=20 thickness=10 color=Black bold hide");
This works for images with 2776x2080px.