diff --git a/materials/29-pathogenwatch.md b/materials/29-pathogenwatch.md index 6f5fe06..1d7659a 100644 --- a/materials/29-pathogenwatch.md +++ b/materials/29-pathogenwatch.md @@ -16,9 +16,9 @@ Whilst you can upload FASTQ files to Pathogenwatch, it's quicker if we work with - Upload the assembled _S. pneumoniae_ genomes to `Pathogenwatch`. - Once `Pathogenwatch` has finished processing the genomes, save the results to a collection called **Chaguza Serotype 1**. -- Download the Typing and AMR profile tables to the `Course_materials/S_pneumoniae` directory. +- Download the Typing and AMR profile tables to the `S_pneumoniae` directory. - Rename the tables to `chaguza-serotype-1-typing.csv` and `chaguza-serotype-1-amr-profile.csv` respectively. -- Merge the two tables with `sample_info.csv` by running the `merge_pneumo_data.py` script in the `scripts` directory. +- Merge the two tables with `sample_info.csv` by running the `merge_pneumo_data.py` script in the `scripts` directory. Make sure you are on the `base` software environment. :::{.callout-hint} @@ -36,7 +36,7 @@ Refer back to [Pathogenwatch](29-pathogenwatch.md) if you need a reminder on how - We clicked on the **download** icon in the top right-hand corner and selected **Typing table**. - We clicked on the **download** icon in the top right-hand corner and selected **AMR profile**. - We renamed the files to `chaguza-serotype-1-typing.csv` and `chaguza-serotype-1-amr-profile.csv` respectively and moved them to the `S_pneumoniae` directory. -- We activated the `tb-profiler` software environment with `mamba activate tb-profiler` +- We went back to the `base` software environment with `mamba activate base` - We ran the `merge_pneumo_data.py` script to create a TSV file called `pneumo_metadata.tsv` in your analysis directory: ```bash