From 0b52043f22dff09bebef07f1878a84d4833da5b4 Mon Sep 17 00:00:00 2001
From: GitHub Actions <actions@github.com>
Date: Mon, 18 Sep 2023 11:48:45 +0000
Subject: [PATCH] site deploy

Auto-generated via {sandpaper}
Source  : 3ac61252305c3e6b38e077b4a5c4691f8c6278dc
Branch  : md-outputs
Author  : GitHub Actions <actions@github.com>
Time    : 2023-09-18 11:48:23 +0000
Message : markdown source builds

Auto-generated via {sandpaper}
Source  : f1daa18469787dc6ecb3dbc16fc7fef90fa8bb7e
Branch  : main
Author  : Charlotte Soneson <charlottesoneson@gmail.com>
Time    : 2023-09-18 11:45:03 +0000
Message : Merge pull request #84 from carpentries-incubator/make-sce-for-isee

Add some more things to the object before calling iSEE
---
 01-intro-to-rnaseq.html                       |   2 +-
 02-setup.html                                 |   2 +-
 03-import-annotate.html                       |   2 +-
 04-exploratory-qc.html                        |  20 ++++++++++++++----
 05-differential-expression.html               |   2 +-
 06-extra-design.html                          |   2 +-
 07-gene-set-analysis.html                     |   2 +-
 08-next-steps.html                            |   2 +-
 404.html                                      |   4 ++--
 CODE_OF_CONDUCT.html                          |   2 +-
 LICENSE.html                                  |   2 +-
 aio.html                                      |  20 ++++++++++++++----
 discuss.html                                  |   2 +-
 ...exploratory-qc-rendered-pca-exercise-1.png | Bin 15030 -> 14926 bytes
 images.html                                   |   4 ++--
 index.html                                    |   4 ++--
 instructor-notes.html                         |   4 ++--
 instructor/01-intro-to-rnaseq.html            |   2 +-
 instructor/02-setup.html                      |   2 +-
 instructor/03-import-annotate.html            |   2 +-
 instructor/04-exploratory-qc.html             |  20 ++++++++++++++----
 instructor/05-differential-expression.html    |   2 +-
 instructor/06-extra-design.html               |   2 +-
 instructor/07-gene-set-analysis.html          |   2 +-
 instructor/08-next-steps.html                 |   2 +-
 instructor/404.html                           |   4 ++--
 instructor/CODE_OF_CONDUCT.html               |   2 +-
 instructor/LICENSE.html                       |   2 +-
 instructor/aio.html                           |  20 ++++++++++++++----
 instructor/discuss.html                       |   2 +-
 instructor/images.html                        |   4 ++--
 instructor/index.html                         |   4 ++--
 instructor/instructor-notes.html              |   4 ++--
 instructor/key-points.html                    |   4 ++--
 instructor/profiles.html                      |   4 ++--
 instructor/reference.html                     |   2 +-
 key-points.html                               |   4 ++--
 md5sum.txt                                    |   2 +-
 pkgdown.yml                                   |   2 +-
 profiles.html                                 |   4 ++--
 reference.html                                |   2 +-
 41 files changed, 112 insertions(+), 64 deletions(-)

diff --git a/01-intro-to-rnaseq.html b/01-intro-to-rnaseq.html
index ee409a0a..043cf24c 100644
--- a/01-intro-to-rnaseq.html
+++ b/01-intro-to-rnaseq.html
@@ -660,7 +660,7 @@ <h3 class="callout-title">Keypoints<a class="anchor" aria-label="anchor" href="#
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/01-intro-to-rnaseq.html",
   "dateCreated": "2020-09-15",
   "dateModified": "2023-09-13",
-  "datePublished": "2023-09-17"
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/02-setup.html b/02-setup.html
index aef24d96..e9a5fde9 100644
--- a/02-setup.html
+++ b/02-setup.html
@@ -657,7 +657,7 @@ <h3 class="callout-title">Keypoints<a class="anchor" aria-label="anchor" href="#
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/02-setup.html",
   "dateCreated": "2020-09-15",
   "dateModified": "2023-09-13",
-  "datePublished": "2023-09-17"
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/03-import-annotate.html b/03-import-annotate.html
index 1d1ddefd..28884c7f 100644
--- a/03-import-annotate.html
+++ b/03-import-annotate.html
@@ -1432,7 +1432,7 @@ <h3 class="callout-title">Keypoints<a class="anchor" aria-label="anchor" href="#
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/03-import-annotate.html",
   "dateCreated": "2020-09-15",
   "dateModified": "2023-09-17",
-  "datePublished": "2023-09-17"
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/04-exploratory-qc.html b/04-exploratory-qc.html
index 6957c94b..147edc0d 100644
--- a/04-exploratory-qc.html
+++ b/04-exploratory-qc.html
@@ -289,7 +289,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Exploratory analysis and quality control</h1>
-        <p> Last updated on 2023-09-17 | 
+        <p> Last updated on 2023-09-18 | 
         
         <a href="https://github.com/carpentries-incubator/bioc-rnaseq/edit/main/episodes/04-exploratory-qc.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
         
@@ -784,7 +784,19 @@ <h3 class="callout-title">Challenge: Interactively explore our data
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
-<code class="sourceCode R"><span><span class="va">app</span> <span class="op">&lt;-</span> <span class="fu">iSEE</span><span class="op">(</span><span class="va">se</span><span class="op">)</span></span>
+<code class="sourceCode R"><span><span class="co">## Convert DESeqDataSet object to a SingleCellExperiment object, in order to </span></span>
+<span><span class="co">## be able to store the PCA representation</span></span>
+<span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">as</span><span class="op">(</span><span class="va">dds</span>, <span class="st">"SingleCellExperiment"</span><span class="op">)</span></span>
+<span></span>
+<span><span class="co">## Add PCA to the 'reducedDim' slot</span></span>
+<span><span class="fu">stopifnot</span><span class="op">(</span><span class="fu">rownames</span><span class="op">(</span><span class="va">pcaData</span><span class="op">)</span> <span class="op">==</span> <span class="fu">colnames</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span><span class="op">)</span></span>
+<span><span class="fu">reducedDim</span><span class="op">(</span><span class="va">sce</span>, <span class="st">"PCA"</span><span class="op">)</span> <span class="op">&lt;-</span> <span class="fu">as.matrix</span><span class="op">(</span><span class="va">pcaData</span><span class="op">[</span>, <span class="fu">c</span><span class="op">(</span><span class="st">"PC1"</span>, <span class="st">"PC2"</span><span class="op">)</span><span class="op">]</span><span class="op">)</span></span>
+<span></span>
+<span><span class="co">## Add variance-stabilized data as a new assay</span></span>
+<span><span class="fu">stopifnot</span><span class="op">(</span><span class="fu">colnames</span><span class="op">(</span><span class="va">vsd</span><span class="op">)</span> <span class="op">==</span> <span class="fu">colnames</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span><span class="op">)</span></span>
+<span><span class="fu">assay</span><span class="op">(</span><span class="va">sce</span>, <span class="st">"vsd"</span><span class="op">)</span> <span class="op">&lt;-</span> <span class="fu">assay</span><span class="op">(</span><span class="va">vsd</span><span class="op">)</span></span>
+<span></span>
+<span><span class="va">app</span> <span class="op">&lt;-</span> <span class="fu">iSEE</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span></span>
 <span><span class="fu">shiny</span><span class="fu">::</span><span class="fu">runApp</span><span class="op">(</span><span class="va">app</span><span class="op">)</span></span></code></pre>
 </div>
 </div>
@@ -937,8 +949,8 @@ <h3 class="callout-title">Keypoints<a class="anchor" aria-label="anchor" href="#
   "url": "https://carpentries-incubator.github.io/bioc-rnaseq/04-exploratory-qc.html",
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/04-exploratory-qc.html",
   "dateCreated": "2020-09-15",
-  "dateModified": "2023-09-17",
-  "datePublished": "2023-09-17"
+  "dateModified": "2023-09-18",
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/05-differential-expression.html b/05-differential-expression.html
index de8bc022..e5d5ebfb 100644
--- a/05-differential-expression.html
+++ b/05-differential-expression.html
@@ -1031,7 +1031,7 @@ <h3 class="callout-title">Keypoints<a class="anchor" aria-label="anchor" href="#
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/05-differential-expression.html",
   "dateCreated": "2020-09-15",
   "dateModified": "2023-09-17",
-  "datePublished": "2023-09-17"
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/06-extra-design.html b/06-extra-design.html
index b3408f92..8f010df0 100644
--- a/06-extra-design.html
+++ b/06-extra-design.html
@@ -2003,7 +2003,7 @@ <h3 class="callout-title">Keypoints<a class="anchor" aria-label="anchor" href="#
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/06-extra-design.html",
   "dateCreated": "2020-09-15",
   "dateModified": "2023-09-13",
-  "datePublished": "2023-09-17"
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/07-gene-set-analysis.html b/07-gene-set-analysis.html
index fed9d440..0eeac49a 100644
--- a/07-gene-set-analysis.html
+++ b/07-gene-set-analysis.html
@@ -2649,7 +2649,7 @@ <h3 class="callout-title">Keypoints<a class="anchor" aria-label="anchor" href="#
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/07-gene-set-analysis.html",
   "dateCreated": "2020-09-15",
   "dateModified": "2023-09-17",
-  "datePublished": "2023-09-17"
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/08-next-steps.html b/08-next-steps.html
index 39d88c7b..7a5cf702 100644
--- a/08-next-steps.html
+++ b/08-next-steps.html
@@ -433,7 +433,7 @@ <h3 class="callout-title">Keypoints<a class="anchor" aria-label="anchor" href="#
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/08-next-steps.html",
   "dateCreated": "2020-09-15",
   "dateModified": "2023-09-13",
-  "datePublished": "2023-09-17"
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/404.html b/404.html
index 5057025a..cca9c03c 100644
--- a/404.html
+++ b/404.html
@@ -322,8 +322,8 @@ <h1>Page not found</h1>
   "url": "https://carpentries-incubator.github.io/bioc-rnaseq/404.html",
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/404.html",
   "dateCreated": "2020-09-15",
-  "dateModified": "2023-09-17",
-  "datePublished": "2023-09-17"
+  "dateModified": "2023-09-18",
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/CODE_OF_CONDUCT.html b/CODE_OF_CONDUCT.html
index 0897e363..e03d8065 100644
--- a/CODE_OF_CONDUCT.html
+++ b/CODE_OF_CONDUCT.html
@@ -346,7 +346,7 @@ <h1>Contributor Code of Conduct</h1>
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/CODE_OF_CONDUCT.html",
   "dateCreated": "2020-09-15",
   "dateModified": "2023-09-13",
-  "datePublished": "2023-09-17"
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/LICENSE.html b/LICENSE.html
index 152e64a4..e4bf8d0f 100644
--- a/LICENSE.html
+++ b/LICENSE.html
@@ -397,7 +397,7 @@ <h1>Licenses</h1>
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/LICENSE.html",
   "dateCreated": "2020-09-15",
   "dateModified": "2023-09-13",
-  "datePublished": "2023-09-17"
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/aio.html b/aio.html
index 94227a18..e3e320af 100644
--- a/aio.html
+++ b/aio.html
@@ -2143,7 +2143,7 @@ <h3 class="callout-title">Keypoints<a class="anchor" aria-label="anchor" href="#
 </div>
 </section></section><section id="aio-04-exploratory-qc"><p>Content from <a href="04-exploratory-qc.html">Exploratory analysis and quality control</a></p>
 <hr>
-<p> Last updated on 2023-09-17 | 
+<p> Last updated on 2023-09-18 | 
         
         <a href="https://github.com/carpentries-incubator/bioc-rnaseq/edit/main/episodes/04-exploratory-qc.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 <div class="text-end">
@@ -2660,7 +2660,19 @@ <h3 class="callout-title">Challenge: Interactively explore our data
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
-<code class="sourceCode R"><span><span class="va">app</span> <span class="op">&lt;-</span> <span class="fu">iSEE</span><span class="op">(</span><span class="va">se</span><span class="op">)</span></span>
+<code class="sourceCode R"><span><span class="co">## Convert DESeqDataSet object to a SingleCellExperiment object, in order to </span></span>
+<span><span class="co">## be able to store the PCA representation</span></span>
+<span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">as</span><span class="op">(</span><span class="va">dds</span>, <span class="st">"SingleCellExperiment"</span><span class="op">)</span></span>
+<span></span>
+<span><span class="co">## Add PCA to the 'reducedDim' slot</span></span>
+<span><span class="fu">stopifnot</span><span class="op">(</span><span class="fu">rownames</span><span class="op">(</span><span class="va">pcaData</span><span class="op">)</span> <span class="op">==</span> <span class="fu">colnames</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span><span class="op">)</span></span>
+<span><span class="fu">reducedDim</span><span class="op">(</span><span class="va">sce</span>, <span class="st">"PCA"</span><span class="op">)</span> <span class="op">&lt;-</span> <span class="fu">as.matrix</span><span class="op">(</span><span class="va">pcaData</span><span class="op">[</span>, <span class="fu">c</span><span class="op">(</span><span class="st">"PC1"</span>, <span class="st">"PC2"</span><span class="op">)</span><span class="op">]</span><span class="op">)</span></span>
+<span></span>
+<span><span class="co">## Add variance-stabilized data as a new assay</span></span>
+<span><span class="fu">stopifnot</span><span class="op">(</span><span class="fu">colnames</span><span class="op">(</span><span class="va">vsd</span><span class="op">)</span> <span class="op">==</span> <span class="fu">colnames</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span><span class="op">)</span></span>
+<span><span class="fu">assay</span><span class="op">(</span><span class="va">sce</span>, <span class="st">"vsd"</span><span class="op">)</span> <span class="op">&lt;-</span> <span class="fu">assay</span><span class="op">(</span><span class="va">vsd</span><span class="op">)</span></span>
+<span></span>
+<span><span class="va">app</span> <span class="op">&lt;-</span> <span class="fu">iSEE</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span></span>
 <span><span class="fu">shiny</span><span class="fu">::</span><span class="fu">runApp</span><span class="op">(</span><span class="va">app</span><span class="op">)</span></span></code></pre>
 </div>
 </div>
@@ -7713,8 +7725,8 @@ <h3 class="callout-title">Keypoints<a class="anchor" aria-label="anchor" href="#
   "url": "https://carpentries-incubator.github.io/bioc-rnaseq/aio.html",
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/aio.html",
   "dateCreated": "2020-09-15",
-  "dateModified": "2023-09-17",
-  "datePublished": "2023-09-17"
+  "dateModified": "2023-09-18",
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/discuss.html b/discuss.html
index 827f40f6..0a2420c1 100644
--- a/discuss.html
+++ b/discuss.html
@@ -339,7 +339,7 @@ <h1>Discussion</h1>
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/discuss.html",
   "dateCreated": "2020-09-15",
   "dateModified": "2023-09-13",
-  "datePublished": "2023-09-17"
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/fig/04-exploratory-qc-rendered-pca-exercise-1.png b/fig/04-exploratory-qc-rendered-pca-exercise-1.png
index cb214694b8482853d2df8becbf2efb08f4ffa1d6..621b3b259384b0e32c0de5640e98ea9d9f353f07 100644
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diff --git a/images.html b/images.html
index 2c61cabc..0671c5ec 100644
--- a/images.html
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@@ -522,8 +522,8 @@ <h3 id="07-gene-set-analysis-figure-12">Figure 12</h3>
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   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/images.html",
   "dateCreated": "2020-09-15",
-  "dateModified": "2023-09-17",
-  "datePublished": "2023-09-17"
+  "dateModified": "2023-09-18",
+  "datePublished": "2023-09-18"
 }
 
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diff --git a/index.html b/index.html
index 78241cc8..23e84c7e 100644
--- a/index.html
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   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/index.html",
   "dateCreated": "2020-09-15",
-  "dateModified": "2023-09-17",
-  "datePublished": "2023-09-17"
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 }
 
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diff --git a/instructor-notes.html b/instructor-notes.html
index 80b89802..b905121f 100644
--- a/instructor-notes.html
+++ b/instructor-notes.html
@@ -384,8 +384,8 @@ <h1>Instructor Notes</h1>
   "url": "https://carpentries-incubator.github.io/bioc-rnaseq/instructor-notes.html",
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/instructor-notes.html",
   "dateCreated": "2020-09-15",
-  "dateModified": "2023-09-17",
-  "datePublished": "2023-09-17"
+  "dateModified": "2023-09-18",
+  "datePublished": "2023-09-18"
 }
 
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diff --git a/instructor/01-intro-to-rnaseq.html b/instructor/01-intro-to-rnaseq.html
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   "dateCreated": "2020-09-15",
   "dateModified": "2023-09-13",
-  "datePublished": "2023-09-17"
+  "datePublished": "2023-09-18"
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diff --git a/instructor/02-setup.html b/instructor/02-setup.html
index 38db0816..c10d90ba 100644
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@@ -658,7 +658,7 @@ <h3 class="callout-title">Keypoints<a class="anchor" aria-label="anchor" href="#
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   "dateCreated": "2020-09-15",
   "dateModified": "2023-09-13",
-  "datePublished": "2023-09-17"
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diff --git a/instructor/03-import-annotate.html b/instructor/03-import-annotate.html
index edfd9673..1ccf4e8c 100644
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+++ b/instructor/03-import-annotate.html
@@ -1433,7 +1433,7 @@ <h3 class="callout-title">Keypoints<a class="anchor" aria-label="anchor" href="#
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   "dateCreated": "2020-09-15",
   "dateModified": "2023-09-17",
-  "datePublished": "2023-09-17"
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diff --git a/instructor/04-exploratory-qc.html b/instructor/04-exploratory-qc.html
index b52541fe..a492c2ef 100644
--- a/instructor/04-exploratory-qc.html
+++ b/instructor/04-exploratory-qc.html
@@ -288,7 +288,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Exploratory analysis and quality control</h1>
-        <p> Last updated on 2023-09-17 | 
+        <p> Last updated on 2023-09-18 | 
         
         <a href="https://github.com/carpentries-incubator/bioc-rnaseq/edit/main/episodes/04-exploratory-qc.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
         
@@ -785,7 +785,19 @@ <h3 class="callout-title">Challenge: Interactively explore our data
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
-<code class="sourceCode R"><span><span class="va">app</span> <span class="op">&lt;-</span> <span class="fu">iSEE</span><span class="op">(</span><span class="va">se</span><span class="op">)</span></span>
+<code class="sourceCode R"><span><span class="co">## Convert DESeqDataSet object to a SingleCellExperiment object, in order to </span></span>
+<span><span class="co">## be able to store the PCA representation</span></span>
+<span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">as</span><span class="op">(</span><span class="va">dds</span>, <span class="st">"SingleCellExperiment"</span><span class="op">)</span></span>
+<span></span>
+<span><span class="co">## Add PCA to the 'reducedDim' slot</span></span>
+<span><span class="fu">stopifnot</span><span class="op">(</span><span class="fu">rownames</span><span class="op">(</span><span class="va">pcaData</span><span class="op">)</span> <span class="op">==</span> <span class="fu">colnames</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span><span class="op">)</span></span>
+<span><span class="fu">reducedDim</span><span class="op">(</span><span class="va">sce</span>, <span class="st">"PCA"</span><span class="op">)</span> <span class="op">&lt;-</span> <span class="fu">as.matrix</span><span class="op">(</span><span class="va">pcaData</span><span class="op">[</span>, <span class="fu">c</span><span class="op">(</span><span class="st">"PC1"</span>, <span class="st">"PC2"</span><span class="op">)</span><span class="op">]</span><span class="op">)</span></span>
+<span></span>
+<span><span class="co">## Add variance-stabilized data as a new assay</span></span>
+<span><span class="fu">stopifnot</span><span class="op">(</span><span class="fu">colnames</span><span class="op">(</span><span class="va">vsd</span><span class="op">)</span> <span class="op">==</span> <span class="fu">colnames</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span><span class="op">)</span></span>
+<span><span class="fu">assay</span><span class="op">(</span><span class="va">sce</span>, <span class="st">"vsd"</span><span class="op">)</span> <span class="op">&lt;-</span> <span class="fu">assay</span><span class="op">(</span><span class="va">vsd</span><span class="op">)</span></span>
+<span></span>
+<span><span class="va">app</span> <span class="op">&lt;-</span> <span class="fu">iSEE</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span></span>
 <span><span class="fu">shiny</span><span class="fu">::</span><span class="fu">runApp</span><span class="op">(</span><span class="va">app</span><span class="op">)</span></span></code></pre>
 </div>
 </div>
@@ -938,8 +950,8 @@ <h3 class="callout-title">Keypoints<a class="anchor" aria-label="anchor" href="#
   "url": "https://carpentries-incubator.github.io/bioc-rnaseq/instructor/04-exploratory-qc.html",
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/instructor/04-exploratory-qc.html",
   "dateCreated": "2020-09-15",
-  "dateModified": "2023-09-17",
-  "datePublished": "2023-09-17"
+  "dateModified": "2023-09-18",
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/instructor/05-differential-expression.html b/instructor/05-differential-expression.html
index 88b8e560..4807f509 100644
--- a/instructor/05-differential-expression.html
+++ b/instructor/05-differential-expression.html
@@ -1103,7 +1103,7 @@ <h3 class="callout-title">Keypoints<a class="anchor" aria-label="anchor" href="#
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   "dateCreated": "2020-09-15",
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-  "datePublished": "2023-09-17"
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diff --git a/instructor/06-extra-design.html b/instructor/06-extra-design.html
index 7869f598..e5080492 100644
--- a/instructor/06-extra-design.html
+++ b/instructor/06-extra-design.html
@@ -2004,7 +2004,7 @@ <h3 class="callout-title">Keypoints<a class="anchor" aria-label="anchor" href="#
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   "dateCreated": "2020-09-15",
   "dateModified": "2023-09-13",
-  "datePublished": "2023-09-17"
+  "datePublished": "2023-09-18"
 }
 
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diff --git a/instructor/07-gene-set-analysis.html b/instructor/07-gene-set-analysis.html
index 455f6412..04a6cbf5 100644
--- a/instructor/07-gene-set-analysis.html
+++ b/instructor/07-gene-set-analysis.html
@@ -2650,7 +2650,7 @@ <h3 class="callout-title">Keypoints<a class="anchor" aria-label="anchor" href="#
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   "dateCreated": "2020-09-15",
   "dateModified": "2023-09-17",
-  "datePublished": "2023-09-17"
+  "datePublished": "2023-09-18"
 }
 
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diff --git a/instructor/08-next-steps.html b/instructor/08-next-steps.html
index 3e7caefb..a044d957 100644
--- a/instructor/08-next-steps.html
+++ b/instructor/08-next-steps.html
@@ -434,7 +434,7 @@ <h3 class="callout-title">Keypoints<a class="anchor" aria-label="anchor" href="#
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/instructor/08-next-steps.html",
   "dateCreated": "2020-09-15",
   "dateModified": "2023-09-13",
-  "datePublished": "2023-09-17"
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/instructor/404.html b/instructor/404.html
index e6425f00..5d3ba61c 100644
--- a/instructor/404.html
+++ b/instructor/404.html
@@ -322,8 +322,8 @@ <h1>Page not found</h1>
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   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/instructor/404.html",
   "dateCreated": "2020-09-15",
-  "dateModified": "2023-09-17",
-  "datePublished": "2023-09-17"
+  "dateModified": "2023-09-18",
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 }
 
   </script><script>
diff --git a/instructor/CODE_OF_CONDUCT.html b/instructor/CODE_OF_CONDUCT.html
index e78d5393..eb82d9bf 100644
--- a/instructor/CODE_OF_CONDUCT.html
+++ b/instructor/CODE_OF_CONDUCT.html
@@ -347,7 +347,7 @@ <h1>Contributor Code of Conduct</h1>
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   "dateCreated": "2020-09-15",
   "dateModified": "2023-09-13",
-  "datePublished": "2023-09-17"
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/instructor/LICENSE.html b/instructor/LICENSE.html
index ceaa37f2..84a56fe7 100644
--- a/instructor/LICENSE.html
+++ b/instructor/LICENSE.html
@@ -398,7 +398,7 @@ <h1>Licenses</h1>
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/instructor/LICENSE.html",
   "dateCreated": "2020-09-15",
   "dateModified": "2023-09-13",
-  "datePublished": "2023-09-17"
+  "datePublished": "2023-09-18"
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   </script><script>
diff --git a/instructor/aio.html b/instructor/aio.html
index 301651d0..4d3ba656 100644
--- a/instructor/aio.html
+++ b/instructor/aio.html
@@ -2142,7 +2142,7 @@ <h3 class="callout-title">Keypoints<a class="anchor" aria-label="anchor" href="#
 </div>
 </section></section><section id="aio-04-exploratory-qc"><p>Content from <a href="04-exploratory-qc.html">Exploratory analysis and quality control</a></p>
 <hr>
-<p> Last updated on 2023-09-17 | 
+<p> Last updated on 2023-09-18 | 
         
         <a href="https://github.com/carpentries-incubator/bioc-rnaseq/edit/main/episodes/04-exploratory-qc.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 <p>Estimated time <i aria-hidden="true" data-feather="clock"></i> 105 minutes </p>
@@ -2660,7 +2660,19 @@ <h3 class="callout-title">Challenge: Interactively explore our data
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
-<code class="sourceCode R"><span><span class="va">app</span> <span class="op">&lt;-</span> <span class="fu">iSEE</span><span class="op">(</span><span class="va">se</span><span class="op">)</span></span>
+<code class="sourceCode R"><span><span class="co">## Convert DESeqDataSet object to a SingleCellExperiment object, in order to </span></span>
+<span><span class="co">## be able to store the PCA representation</span></span>
+<span><span class="va">sce</span> <span class="op">&lt;-</span> <span class="fu">as</span><span class="op">(</span><span class="va">dds</span>, <span class="st">"SingleCellExperiment"</span><span class="op">)</span></span>
+<span></span>
+<span><span class="co">## Add PCA to the 'reducedDim' slot</span></span>
+<span><span class="fu">stopifnot</span><span class="op">(</span><span class="fu">rownames</span><span class="op">(</span><span class="va">pcaData</span><span class="op">)</span> <span class="op">==</span> <span class="fu">colnames</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span><span class="op">)</span></span>
+<span><span class="fu">reducedDim</span><span class="op">(</span><span class="va">sce</span>, <span class="st">"PCA"</span><span class="op">)</span> <span class="op">&lt;-</span> <span class="fu">as.matrix</span><span class="op">(</span><span class="va">pcaData</span><span class="op">[</span>, <span class="fu">c</span><span class="op">(</span><span class="st">"PC1"</span>, <span class="st">"PC2"</span><span class="op">)</span><span class="op">]</span><span class="op">)</span></span>
+<span></span>
+<span><span class="co">## Add variance-stabilized data as a new assay</span></span>
+<span><span class="fu">stopifnot</span><span class="op">(</span><span class="fu">colnames</span><span class="op">(</span><span class="va">vsd</span><span class="op">)</span> <span class="op">==</span> <span class="fu">colnames</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span><span class="op">)</span></span>
+<span><span class="fu">assay</span><span class="op">(</span><span class="va">sce</span>, <span class="st">"vsd"</span><span class="op">)</span> <span class="op">&lt;-</span> <span class="fu">assay</span><span class="op">(</span><span class="va">vsd</span><span class="op">)</span></span>
+<span></span>
+<span><span class="va">app</span> <span class="op">&lt;-</span> <span class="fu">iSEE</span><span class="op">(</span><span class="va">sce</span><span class="op">)</span></span>
 <span><span class="fu">shiny</span><span class="fu">::</span><span class="fu">runApp</span><span class="op">(</span><span class="va">app</span><span class="op">)</span></span></code></pre>
 </div>
 </div>
@@ -7788,8 +7800,8 @@ <h3 class="callout-title">Keypoints<a class="anchor" aria-label="anchor" href="#
   "url": "https://carpentries-incubator.github.io/bioc-rnaseq/instructor/aio.html",
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/instructor/aio.html",
   "dateCreated": "2020-09-15",
-  "dateModified": "2023-09-17",
-  "datePublished": "2023-09-17"
+  "dateModified": "2023-09-18",
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/instructor/discuss.html b/instructor/discuss.html
index 2422d3df..919f5b77 100644
--- a/instructor/discuss.html
+++ b/instructor/discuss.html
@@ -340,7 +340,7 @@ <h1>Discussion</h1>
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/instructor/discuss.html",
   "dateCreated": "2020-09-15",
   "dateModified": "2023-09-13",
-  "datePublished": "2023-09-17"
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/instructor/images.html b/instructor/images.html
index e4a4cee5..dd1ff2d5 100644
--- a/instructor/images.html
+++ b/instructor/images.html
@@ -518,8 +518,8 @@ <h3 id="07-gene-set-analysis-figure-12">Figure 12</h3>
   "url": "https://carpentries-incubator.github.io/bioc-rnaseq/instructor/images.html",
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/instructor/images.html",
   "dateCreated": "2020-09-15",
-  "dateModified": "2023-09-17",
-  "datePublished": "2023-09-17"
+  "dateModified": "2023-09-18",
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/instructor/index.html b/instructor/index.html
index f90776e4..9da8fda5 100644
--- a/instructor/index.html
+++ b/instructor/index.html
@@ -488,8 +488,8 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
   "url": "https://carpentries-incubator.github.io/bioc-rnaseq/instructor/index.html",
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/instructor/index.html",
   "dateCreated": "2020-09-15",
-  "dateModified": "2023-09-17",
-  "datePublished": "2023-09-17"
+  "dateModified": "2023-09-18",
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/instructor/instructor-notes.html b/instructor/instructor-notes.html
index c5ba133a..9801b563 100644
--- a/instructor/instructor-notes.html
+++ b/instructor/instructor-notes.html
@@ -433,8 +433,8 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
   "url": "https://carpentries-incubator.github.io/bioc-rnaseq/instructor/instructor-notes.html",
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/instructor/instructor-notes.html",
   "dateCreated": "2020-09-15",
-  "dateModified": "2023-09-17",
-  "datePublished": "2023-09-17"
+  "dateModified": "2023-09-18",
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/instructor/key-points.html b/instructor/key-points.html
index b2810b46..53924a81 100644
--- a/instructor/key-points.html
+++ b/instructor/key-points.html
@@ -457,8 +457,8 @@ <h2 class="accordion-header" id="flush-headingTwelve">
   "url": "https://carpentries-incubator.github.io/bioc-rnaseq/instructor/key-points.html",
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/instructor/key-points.html",
   "dateCreated": "2020-09-15",
-  "dateModified": "2023-09-17",
-  "datePublished": "2023-09-17"
+  "dateModified": "2023-09-18",
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/instructor/profiles.html b/instructor/profiles.html
index f0bb4597..ef898ac5 100644
--- a/instructor/profiles.html
+++ b/instructor/profiles.html
@@ -383,8 +383,8 @@ <h1>Learner Profiles</h1>
   "url": "https://carpentries-incubator.github.io/bioc-rnaseq/instructor/profiles.html",
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/instructor/profiles.html",
   "dateCreated": "2020-09-15",
-  "dateModified": "2023-09-17",
-  "datePublished": "2023-09-17"
+  "dateModified": "2023-09-18",
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/instructor/reference.html b/instructor/reference.html
index f567c9cd..24d8d81d 100644
--- a/instructor/reference.html
+++ b/instructor/reference.html
@@ -340,7 +340,7 @@ <h1></h1>
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/instructor/reference.html",
   "dateCreated": "2020-09-15",
   "dateModified": "2023-09-13",
-  "datePublished": "2023-09-17"
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/key-points.html b/key-points.html
index bd6b09b1..cfcc2b10 100644
--- a/key-points.html
+++ b/key-points.html
@@ -461,8 +461,8 @@ <h2 class="accordion-header" id="flush-headingTwelve">
   "url": "https://carpentries-incubator.github.io/bioc-rnaseq/key-points.html",
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/key-points.html",
   "dateCreated": "2020-09-15",
-  "dateModified": "2023-09-17",
-  "datePublished": "2023-09-17"
+  "dateModified": "2023-09-18",
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/md5sum.txt b/md5sum.txt
index 828c1476..606cbb26 100644
--- a/md5sum.txt
+++ b/md5sum.txt
@@ -6,7 +6,7 @@
 "episodes/01-intro-to-rnaseq.Rmd" "7cc3f4922347f6d5d9ef80dd31e84fbb" "site/built/01-intro-to-rnaseq.md" "2023-09-13"
 "episodes/02-setup.Rmd" "44ede89e11c54f154bacc1f5d4ee01f2" "site/built/02-setup.md" "2023-09-13"
 "episodes/03-import-annotate.Rmd" "f07c9513607ea7cbcd8041bf9bfd5f0e" "site/built/03-import-annotate.md" "2023-09-17"
-"episodes/04-exploratory-qc.Rmd" "f4b0f8f545ff869019c245b1638af48d" "site/built/04-exploratory-qc.md" "2023-09-17"
+"episodes/04-exploratory-qc.Rmd" "59d9cac581d1c736628534f8cf37f1b5" "site/built/04-exploratory-qc.md" "2023-09-18"
 "episodes/05-differential-expression.Rmd" "29ab3939367a0b3521cfd764929e3aee" "site/built/05-differential-expression.md" "2023-09-17"
 "episodes/06-extra-design.Rmd" "e4859b8e76bc6d0c8d5c598e1d00b37b" "site/built/06-extra-design.md" "2023-09-13"
 "episodes/07-gene-set-analysis.Rmd" "e7b69b6f2af11dfab883303e9a50981d" "site/built/07-gene-set-analysis.md" "2023-09-17"
diff --git a/pkgdown.yml b/pkgdown.yml
index 234f3c8d..320dd7cd 100644
--- a/pkgdown.yml
+++ b/pkgdown.yml
@@ -2,5 +2,5 @@ pandoc: 2.19.2
 pkgdown: 2.0.7
 pkgdown_sha: ~
 articles: {}
-last_built: 2023-09-17T17:41Z
+last_built: 2023-09-18T11:48Z
 
diff --git a/profiles.html b/profiles.html
index 64164666..df228532 100644
--- a/profiles.html
+++ b/profiles.html
@@ -384,8 +384,8 @@ <h1>Learner Profiles</h1>
   "url": "https://carpentries-incubator.github.io/bioc-rnaseq/profiles.html",
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/profiles.html",
   "dateCreated": "2020-09-15",
-  "dateModified": "2023-09-17",
-  "datePublished": "2023-09-17"
+  "dateModified": "2023-09-18",
+  "datePublished": "2023-09-18"
 }
 
   </script><script>
diff --git a/reference.html b/reference.html
index 3ec35452..04dd0a2d 100644
--- a/reference.html
+++ b/reference.html
@@ -339,7 +339,7 @@ <h1></h1>
   "identifier": "https://carpentries-incubator.github.io/bioc-rnaseq/reference.html",
   "dateCreated": "2020-09-15",
   "dateModified": "2023-09-13",
-  "datePublished": "2023-09-17"
+  "datePublished": "2023-09-18"
 }
 
   </script><script>