Some of the projects I have worked on, and the ones I am currently tackling.
Created as part of the final project for my Instrumentation class
First functional mason jar reactor
Closeup of vessel with E. Coli expressing RFP
Second reactor version utilizing an Arduino Uno for temperature control
- Sterilizable by autoclave
- Easily dissasembled for educational use
- Made from easily obtained parts
- Used to teach about microbiology, biochemical engineering, and electronics
Quart reactors with E. coli expressing different colors
Same reactors under blacklight
Extracted GFP from reactors that has been polymerized under blacklight. Shaped using a 3D printed dogbone mold.
- Maintain cells in their optimal growth phase by exchanging media
- Measure Optical Density
- Measure pH (optional control)
- Sterilizable by autoclave
- Web browser based control
- Easily sourced parts
Vessel Connected to the tower. Tower is 3D printed.
3 peristaltic pumps and the air pump
Inside the vessel lid. 4 Sample ports, Sparge stone, thermowell, and heater
Current parameters on the screen
Side of the tower with the Optical Density module, and the electrical connections
Assembled vessel
Progression of the reactor vessels. (Left to right) V1, V2 pint, V2 quart, and the chemostat vessel
Bioprinter converted from a i3 clone. 3D printed syringe pump connected on the left side to pump viscous fluids instead of plastics.
Syringe pump was built following this paper. https://www.sciencedirect.com/science/article/pii/S2468067217300822
Glass dish adhered to bed, filled with a gelatin slurry for support of the printed item.
Stand alone 3D Printed syringe pump
Link to github with build details. https://github.com/FOSH-following-demand/Syringe_Pump
- Reduce backlash from previously built pump
- Allow for alternate syringe sizes
- Belted drive reduction to reduce noise from 3D printed gears
