diff --git a/src/Http/Http.Extensions/src/RequestDelegateFactory.cs b/src/Http/Http.Extensions/src/RequestDelegateFactory.cs
index c51e7aae5a36..42e26d2fcf21 100644
--- a/src/Http/Http.Extensions/src/RequestDelegateFactory.cs
+++ b/src/Http/Http.Extensions/src/RequestDelegateFactory.cs
@@ -164,7 +164,10 @@ public static RequestDelegateResult Create(Delegate handler, RequestDelegateFact
     /// <summary>
     /// Creates a <see cref="RequestDelegate"/> implementation for <paramref name="handler"/>.
     /// </summary>
-    /// <param name="handler">A request handler with any number of custom parameters that often produces a response with its return value.</param>
+    /// <param name="handler">
+    /// A request handler with any number of custom parameters that often produces a response with its return value.
+    /// If delegate points to instance method, but <see cref="Delegate.Target"/> is set to <see langword="null"/>, target will be fetched from <seealso cref="HttpContext.RequestServices"/>.
+    /// </param>
     /// <param name="options">The <see cref="RequestDelegateFactoryOptions"/> used to configure the behavior of the handler.</param>
     /// <param name="metadataResult">
     /// The result returned from <see cref="InferMetadata(MethodInfo, RequestDelegateFactoryOptions?)"/> if that was used to inferring metadata before creating the final RequestDelegate.
@@ -178,23 +181,37 @@ public static RequestDelegateResult Create(Delegate handler, RequestDelegateFact
     {
         ArgumentNullException.ThrowIfNull(handler);
 
-        var targetExpression = handler.Target switch
+        UnaryExpression? targetExpression = null;
+        Func<HttpContext, object?>? targetFactory = null;
+        Expression<Func<HttpContext, object?>>? targetFactoryExpression = null;
+
+        switch (handler.Target)
         {
-            object => Expression.Convert(TargetExpr, handler.Target.GetType()),
-            null => null,
-        };
+            case object:
+                targetExpression = Expression.Convert(TargetExpr, handler.Target.GetType());
+                targetFactory = (httpContext) => handler.Target;
+                targetFactoryExpression = (httpContext) => handler.Target;
+
+                break;
+
+            case null when !handler.Method.IsStatic:
+                targetExpression = Expression.Convert(TargetExpr, handler.Method.ReflectedType!);
+                targetFactory = (httpContext) => httpContext.RequestServices.GetRequiredService(handler.Method.ReflectedType!);
+                targetFactoryExpression = (httpContext) => httpContext.RequestServices.GetRequiredService(handler.Method.ReflectedType!);
+
+                break;
+        }
 
         var factoryContext = CreateFactoryContext(options, metadataResult, handler);
 
-        Expression<Func<HttpContext, object?>> targetFactory = (httpContext) => handler.Target;
-        var targetableRequestDelegate = CreateTargetableRequestDelegate(handler.Method, targetExpression, factoryContext, targetFactory);
+        var targetableRequestDelegate = CreateTargetableRequestDelegate(handler.Method, targetExpression, factoryContext, targetFactoryExpression);
 
         RequestDelegate finalRequestDelegate = targetableRequestDelegate switch
         {
             // handler is a RequestDelegate that has not been modified by a filter. Short-circuit and return the original RequestDelegate back.
             // It's possible a filter factory has still modified the endpoint metadata though.
             null => (RequestDelegate)handler,
-            _ => httpContext => targetableRequestDelegate(handler.Target, httpContext),
+            _ => httpContext => targetableRequestDelegate(targetFactory?.Invoke(httpContext), httpContext),
         };
 
         return CreateRequestDelegateResult(finalRequestDelegate, factoryContext.EndpointBuilder);
@@ -369,8 +386,9 @@ private static IReadOnlyList<object> AsReadOnlyList(IList<object> metadata)
             }
         }
 
-        // return null for plain RequestDelegates that have not been modified by filters so we can just pass back the original RequestDelegate.
-        if (filterPipeline is null && factoryContext.Handler is RequestDelegate)
+        // return null for plain RequestDelegates that have not been modified by filters so we can just pass back the original RequestDelegate
+        // but only when target is not injected
+        if (filterPipeline is null && factoryContext.Handler is RequestDelegate && (factoryContext.Handler.Method.IsStatic || factoryContext.Handler.Target is not null))
         {
             return null;
         }
diff --git a/src/Http/Http.Extensions/test/RequestDelegateFactoryTests.cs b/src/Http/Http.Extensions/test/RequestDelegateFactoryTests.cs
index 61e7d2a46965..a872cb3a8624 100644
--- a/src/Http/Http.Extensions/test/RequestDelegateFactoryTests.cs
+++ b/src/Http/Http.Extensions/test/RequestDelegateFactoryTests.cs
@@ -1217,6 +1217,56 @@ public async Task RequestDelegatePopulatesParametersFromServiceWithAndWithoutAtt
         Assert.Same(myOriginalService, httpContext.Items["service"]);
     }
 
+    [Fact]
+    public async Task RequestDelegateInjectingHandlerForUnboundCustomDelegate()
+    {
+        var serviceCollection = new ServiceCollection();
+        serviceCollection.AddSingleton(LoggerFactory);
+        serviceCollection.AddScoped<HttpHandler>();
+
+        var services = serviceCollection.BuildServiceProvider();
+
+        using var requestScoped = services.CreateScope();
+
+        var httpContext = CreateHttpContext();
+        httpContext.RequestServices = requestScoped.ServiceProvider;
+
+        var requestMethod = typeof(HttpHandler).GetMethod(nameof(HttpHandler.Handle))!;
+        var requestMethodDelegate = requestMethod.CreateDelegate<Func<HttpHandler, HttpContext, Task>>();
+
+        var factoryResult = RequestDelegateFactory.Create(requestMethodDelegate, options: new() { ServiceProvider = services });
+        var requestDelegate = factoryResult.RequestDelegate;
+
+        await requestDelegate(httpContext);
+
+        Assert.Equal(1, httpContext.Items["calls"]);
+    }
+
+    [Fact]
+    public async Task RequestDelegateInjectingHandlerForUnboundRequestDelegate()
+    {
+        var serviceCollection = new ServiceCollection();
+        serviceCollection.AddSingleton(LoggerFactory);
+        serviceCollection.AddScoped<HttpHandler>();
+
+        var services = serviceCollection.BuildServiceProvider();
+
+        using var requestScoped = services.CreateScope();
+
+        var httpContext = CreateHttpContext();
+        httpContext.RequestServices = requestScoped.ServiceProvider;
+
+        var requestMethod = typeof(HttpHandler).GetMethod(nameof(HttpHandler.Handle))!;
+        var requestMethodDelegate = requestMethod.CreateDelegate<RequestDelegate>(null);
+
+        var factoryResult = RequestDelegateFactory.Create(requestMethodDelegate, options: new() { ServiceProvider = services });
+        var requestDelegate = factoryResult.RequestDelegate;
+
+        await requestDelegate(httpContext);
+
+        Assert.Equal(1, httpContext.Items["calls"]);
+    }
+
     [Fact]
     public async Task RequestDelegatePopulatesHttpContextParameterWithoutAttribute()
     {
@@ -3659,14 +3709,19 @@ private class FromServiceAttribute : Attribute, IFromServiceMetadata
     {
     }
 
-    class HttpHandler
+    private class HttpHandler
     {
         private int _calls;
 
-        public void Handle(HttpContext httpContext)
+        /// <remarks>
+        /// Method in form of <see cref="RequestDelegate"/>.
+        /// </remarks>
+        public Task Handle(HttpContext httpContext)
         {
             _calls++;
             httpContext.Items["calls"] = _calls;
+
+            return Task.CompletedTask;
         }
     }