diff --git a/erpnext/stock/doctype/pick_list/pick_list.py b/erpnext/stock/doctype/pick_list/pick_list.py
index 6dd53f290c96..066dba95b1e3 100644
--- a/erpnext/stock/doctype/pick_list/pick_list.py
+++ b/erpnext/stock/doctype/pick_list/pick_list.py
@@ -6,7 +6,7 @@
 from itertools import groupby
 
 import frappe
-from frappe import _
+from frappe import _, bold
 from frappe.model.document import Document
 from frappe.model.mapper import map_child_doc
 from frappe.query_builder import Case
@@ -73,6 +73,9 @@ def onload(self) -> None:
 
 	def validate(self):
 		self.validate_for_qty()
+		if self.pick_manually and self.get("locations"):
+			self.validate_stock_qty()
+			self.check_serial_no_status()
 
 	def before_save(self):
 		self.update_status()
@@ -82,6 +85,60 @@ def before_save(self):
 		if self.get("locations"):
 			self.validate_sales_order_percentage()
 
+	def validate_stock_qty(self):
+		from erpnext.stock.doctype.batch.batch import get_batch_qty
+
+		for row in self.get("locations"):
+			if row.batch_no and not row.qty:
+				batch_qty = get_batch_qty(row.batch_no, row.warehouse, row.item_code)
+
+				if row.qty > batch_qty:
+					frappe.throw(
+						_(
+							"At Row #{0}: The picked quantity {1} for the item {2} is greater than available stock {3} for the batch {4} in the warehouse {5}."
+						).format(row.idx, row.item_code, batch_qty, row.batch_no, bold(row.warehouse)),
+						title=_("Insufficient Stock"),
+					)
+
+				continue
+
+			bin_qty = frappe.db.get_value(
+				"Bin",
+				{"item_code": row.item_code, "warehouse": row.warehouse},
+				"actual_qty",
+			)
+
+			if row.qty > bin_qty:
+				frappe.throw(
+					_(
+						"At Row #{0}: The picked quantity {1} for the item {2} is greater than available stock {3} in the warehouse {4}."
+					).format(row.idx, row.qty, bold(row.item_code), bin_qty, bold(row.warehouse)),
+					title=_("Insufficient Stock"),
+				)
+
+	def check_serial_no_status(self):
+		from erpnext.stock.doctype.serial_no.serial_no import get_serial_nos
+
+		for row in self.get("locations"):
+			if not row.serial_no:
+				continue
+
+			picked_serial_nos = get_serial_nos(row.serial_no)
+			validated_serial_nos = frappe.get_all(
+				"Serial No",
+				filters={"name": ("in", picked_serial_nos), "warehouse": row.warehouse},
+				pluck="name",
+			)
+
+			incorrect_serial_nos = set(picked_serial_nos) - set(validated_serial_nos)
+			if incorrect_serial_nos:
+				frappe.throw(
+					_("The Serial No at Row #{0}: {1} is not available in warehouse {2}.").format(
+						row.idx, ", ".join(incorrect_serial_nos), row.warehouse
+					),
+					title=_("Incorrect Warehouse"),
+				)
+
 	def validate_sales_order_percentage(self):
 		# set percentage picked in SO
 		for location in self.get("locations"):
diff --git a/erpnext/stock/doctype/pick_list/test_pick_list.py b/erpnext/stock/doctype/pick_list/test_pick_list.py
index 116a0bd833d9..3341b1fc6de4 100644
--- a/erpnext/stock/doctype/pick_list/test_pick_list.py
+++ b/erpnext/stock/doctype/pick_list/test_pick_list.py
@@ -1132,3 +1132,45 @@ def test_pick_list_for_multiple_sales_orders_for_non_serialized_item(self):
 		pl.save()
 
 		self.assertEqual(pl.locations[0].qty, 80.0)
+
+	def test_validate_picked_qty_with_manual_option(self):
+		warehouse = "_Test Warehouse - _TC"
+		non_serialized_item = make_item(
+			"Test Non Serialized Pick List Item For Manual Option", properties={"is_stock_item": 1}
+		).name
+
+		serialized_item = make_item(
+			"Test Serialized Pick List Item For Manual Option",
+			properties={"is_stock_item": 1, "has_serial_no": 1, "serial_no_series": "SN-HSNMSPLI-.####"},
+		).name
+
+		batched_item = make_item(
+			"Test Batched Pick List Item For Manual Option",
+			properties={
+				"is_stock_item": 1,
+				"has_batch_no": 1,
+				"batch_number_series": "SN-HBNMSPLI-.####",
+				"create_new_batch": 1,
+			},
+		).name
+
+		make_stock_entry(item=non_serialized_item, to_warehouse=warehouse, qty=10, basic_rate=100)
+		make_stock_entry(item=serialized_item, to_warehouse=warehouse, qty=10, basic_rate=100)
+		make_stock_entry(item=batched_item, to_warehouse=warehouse, qty=10, basic_rate=100)
+
+		so = make_sales_order(
+			item_code=non_serialized_item, qty=10, rate=100, do_not_save=True, warehouse=warehouse
+		)
+		so.append("items", {"item_code": serialized_item, "qty": 10, "rate": 100, "warehouse": warehouse})
+		so.append("items", {"item_code": batched_item, "qty": 10, "rate": 100, "warehouse": warehouse})
+		so.set_missing_values()
+		so.save()
+		so.submit()
+
+		pl = create_pick_list(so.name)
+		pl.pick_manually = 1
+
+		for row in pl.locations:
+			row.qty = row.qty + 10
+
+		self.assertRaises(frappe.ValidationError, pl.save)