From 0224a429fdeda1630cbbcb2e0668a77becd56eda Mon Sep 17 00:00:00 2001 From: Cameron Hyde Date: Thu, 29 Aug 2024 08:14:23 +1000 Subject: [PATCH 01/18] Add proteomics lab --- subdomains/proteomics/CONTRIBUTORS | 3 + subdomains/proteomics/base.yml | 38 +++++ .../sections/conversion_modification.yml | 49 ++++++ subdomains/proteomics/sections/data.yml | 55 +++++++ .../sections/database_searching.yml | 78 +++++++++ .../sections/dda_standardised_tools.yml | 71 ++++++++ subdomains/proteomics/sections/dda_tmt.yml | 60 +++++++ .../sections/dia_standardised_tools.yml | 43 +++++ subdomains/proteomics/static/custom.css | 21 +++ subdomains/proteomics/static/logo.png | Bin 0 -> 2845 bytes .../proteomics/templates/conclusion.html | 1 + subdomains/proteomics/templates/footer.html | 9 + subdomains/proteomics/templates/intro.html | 155 ++++++++++++++++++ 13 files changed, 583 insertions(+) create mode 100644 subdomains/proteomics/CONTRIBUTORS create mode 100644 subdomains/proteomics/base.yml create mode 100644 subdomains/proteomics/sections/conversion_modification.yml create mode 100644 subdomains/proteomics/sections/data.yml create mode 100644 subdomains/proteomics/sections/database_searching.yml create mode 100644 subdomains/proteomics/sections/dda_standardised_tools.yml create mode 100644 subdomains/proteomics/sections/dda_tmt.yml create mode 100644 subdomains/proteomics/sections/dia_standardised_tools.yml create mode 100644 subdomains/proteomics/static/custom.css create mode 100644 subdomains/proteomics/static/logo.png create mode 100644 subdomains/proteomics/templates/conclusion.html create mode 100644 subdomains/proteomics/templates/footer.html create mode 100644 subdomains/proteomics/templates/intro.html diff --git a/subdomains/proteomics/CONTRIBUTORS b/subdomains/proteomics/CONTRIBUTORS new file mode 100644 index 00000000..a690e999 --- /dev/null +++ b/subdomains/proteomics/CONTRIBUTORS @@ -0,0 +1,3 @@ +# If GitHub username, name and avatar will be fetched and displayed +supernord +neoformit diff --git a/subdomains/proteomics/base.yml b/subdomains/proteomics/base.yml new file mode 100644 index 00000000..14625c6d --- /dev/null +++ b/subdomains/proteomics/base.yml @@ -0,0 +1,38 @@ +# Test this locally with: +# http://127.0.0.1:8000/lab/export?content_root=http://localhost:8000/static/home/labs/proteomics/base.yml + +# Request this on site.usegalaxy.org.au with: +# https://site.usegalaxy.org.au/lab/export?content_root=https://site.usegalaxy.org.au/static/home/labs/proteomics/base.yml + +# Check out the documentation for building exported labs: +# https://site.usegalaxy.org.au/lab/export + +# Use these variables in HTML templates like: +# "Welcome to the Galaxy {{ site_name }} {{ lab_name }}" +# To make the content more generic and reusable across sites +site_name: Australia +lab_name: Proteomics Lab +nationality: Australian +galaxy_base_url: https://proteomics.usegalaxy.org.au # Use for rendering tool/workflow URLs. Trailing '/' will be removed. +subdomain: proteomics +root_domain: usegalaxy.org.au +feedback_email: help@genome.edu.au + +# Custom content relative to this file URL +header_logo: static/logo.png +custom_css: static/custom.css +intro_md: templates/intro.html +conclusion_md: templates/conclusion.html +footer_md: templates/footer.html + +# Data (Tools, Workflows etc.) to be rendered into sections/tabs/accordion elements. +# Either: +# 1. Relative to this file URL +# 2. Full URL to fetch globally centralized content +sections: + - sections/data.yml + - sections/conversion_modification.yml + - sections/database_searching.yml + - sections/dda_standardised_tools.yml + - sections/dia_standardised_tools.yml + - sections/dda_tmt.yml diff --git a/subdomains/proteomics/sections/conversion_modification.yml b/subdomains/proteomics/sections/conversion_modification.yml new file mode 100644 index 00000000..6a074920 --- /dev/null +++ b/subdomains/proteomics/sections/conversion_modification.yml @@ -0,0 +1,49 @@ +id: conversion_modification +title: MS file conversion +tabs: + - id: tools + title: Tools + heading_md: > + Some example tools are listed here: you can also search for more in the full tool panel to the left. + content: + - title_md: "msconvert" + description_md: > +

+ Convert and/or filter mass spectrometry files. +

+ inputs: + - datatypes: + - thermo.raw + - mzML + - mzXML + - raw + - wiff + - wiff.tar + - agilentbrukeryep.d.tar + - agilentmasshunter.d.tar + - brukerbaf.d.tar + - brukertdf.d.tar + - watersmasslynx.raw.tar + label: Input MS data + outputs: + - datatypes: + - mz5 + - mzML + - mzXML + - mgf + - ms2 + label: Output MS data + button_link: "{{ galaxy_base_url }}/root?tool_id=toolshed.g2.bx.psu.edu/repos/galaxyp/msconvert/msconvert/" + - title_md: "Thermo RAW file converter" + description_md: > +

+ Thermo RAW file converter. +

+ inputs: + - datatypes: + - thermo.raw + label: Thermo RAW file + button_link: "{{ galaxy_base_url }}/root?tool_id=toolshed.g2.bx.psu.edu/repos/galaxyp/thermo_raw_file_converter/thermo_raw_file_converter/" + # - id: help + # title: Help + # content: [] diff --git a/subdomains/proteomics/sections/data.yml b/subdomains/proteomics/sections/data.yml new file mode 100644 index 00000000..b162e648 --- /dev/null +++ b/subdomains/proteomics/sections/data.yml @@ -0,0 +1,55 @@ +id: data +title: Data import +tabs: + - id: tools + title: Tools + heading_md: "Some example tools are listed here: you can also search for more in the full tool panel to the left." + content: + - title_md: "Import data to Galaxy" + description_md: "

Standard upload of data to Galaxy, from your computer or from the web.

" + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=upload1" + - id: help + title: Help + content: + - title_md: "Can I upload sensitive data?" + description_md: | +

+ No, do not upload personal or sensitive, such as human health or clinical data. + Please see our + Data Privacy + page for definitions of sensitive and health-related information. +

+

+ Please also make sure you have read our + Terms of Service, + which covers hosting and analysis of research data. +

+ - title_md: "Is my data private?" + description_md: | +

+ Please read our + Privacy Policy + for information on your personal data and any data that you upload. +

+ - title_md: "How can I increase my storage quota?" + description_md: | +

+ Please submit a quota request if your Galaxy Australia account reaches its data storage limit. Requests are usually provisioned quickly if you provide a reasonable use case for your request. +

+ button_link: "/request/quota" + button_md: "Request" + - title_md: "Tutorial: Introduction to proteomics, protein identification, quantification and statistical modelling" + description_md: | +

+ This practical aims to familiarize you with Galaxy for Proteomics, including theory, methods and software examples. +

+ button_link: "https://training.galaxyproject.org/training-material/topics/proteomics/tutorials/introduction/slides.html#1" + button_md: "Tutorial" + - title_md: "Galaxy Australia support" + description_md: | +

+ Any user of Galaxy Australia can request support through an + online form. +

+ button_link: "/request/support" + button_md: "Request support" diff --git a/subdomains/proteomics/sections/database_searching.yml b/subdomains/proteomics/sections/database_searching.yml new file mode 100644 index 00000000..fb36ab0c --- /dev/null +++ b/subdomains/proteomics/sections/database_searching.yml @@ -0,0 +1,78 @@ +id: database_searching +title: Database searching +tabs: + - id: tools + title: Tools + heading_md: > + Some example tools are listed here: you can also search for more in the full tool panel to the left. + content: + - title_md: "DecoyDatabase" + description_md: > +

+ Create decoy sequence database from forward sequence database. +

+ inputs: + - datatypes: + - fasta + label: Input FASTA file(s), each containing a database + button_link: "{{ galaxy_base_url }}/root?tool_id=toolshed.g2.bx.psu.edu/repos/galaxyp/openms_decoydatabase/DecoyDatabase/" + - title_md: "MaxQuant" + description_md: > +

+ MaxQuant is a quantitative proteomics software package designed for analyzing large mass-spectrometric data sets. +

+ inputs: + - datatypes: + - thermo.raw + - mzML + - mzXML + label: Mass spectrometry data sets + - datatypes: + - tabular + label: Experimental design template + button_link: "{{ galaxy_base_url }}/root?tool_id=toolshed.g2.bx.psu.edu/repos/galaxyp/maxquant/maxquant/" + - title_md: "Morpheus" + description_md: > +

+ Database search algorithm for high-resolution tandem mass spectra. +

+ inputs: + - datatypes: + - mzML + label: Indexed mzML + - datatypes: + - fasta + - uniprotxml + label: "MS Protein Search Database: UniProt Xml or Fasta" + button_link: "{{ galaxy_base_url }}/root?tool_id=toolshed.g2.bx.psu.edu/repos/galaxyp/morpheus/morpheus/" + - id: help + title: Help + content: + - title_md: "Introduction to proteomics, protein identification, quantification and statistical modelling" + description_md: > +

+ Start here if you are new to proteomic analysis in Galaxy. +

+ button_link: "https://usegalaxy.org.au/training-material/topics/proteomics/tutorials/introduction/slides.html" + button_md: "Tutorial" + - title_md: "Label-free data analysis using MaxQuant" + description_md: > +

+ Learn how to use MaxQuant for the analysis of label-free shotgun (DDA) data. +

+ button_link: "https://proteomics.usegalaxy.org.au/training-material/topics/proteomics/tutorials/maxquant-label-free/tutorial.html" + button_md: "Tutorial" + - title_md: "Peptide and Protein ID using OpenMS tools" + description_md: > +

+ Learn how to identify proteins from LC-MS/MS raw files. +

+ button_link: "https://usegalaxy.org.au/training-material/topics/proteomics/tutorials/protein-id-oms/tutorial.html" + button_md: "Tutorial" + - title_md: "Galaxy Australia support" + description_md: > +

+ Any user of Galaxy Australia can request support through an online form. +

+ button_link: "/request/support" + button_md: "Request support" \ No newline at end of file diff --git a/subdomains/proteomics/sections/dda_standardised_tools.yml b/subdomains/proteomics/sections/dda_standardised_tools.yml new file mode 100644 index 00000000..d66139c7 --- /dev/null +++ b/subdomains/proteomics/sections/dda_standardised_tools.yml @@ -0,0 +1,71 @@ +id: dda_standardised_tools +title: DDA Standardised Tools +tabs: + - id: tools + title: Tools + heading_md: > + Some example tools are listed here: you can also search for more in the full tool panel to the left. + content: + - title_md: "MaxQuant" + description_md: > +

+ MaxQuant is a quantitative proteomics software package designed for analyzing large mass-spectrometric data sets. +

+ inputs: + - datatypes: + - thermo.raw + - mzML + - mzXML + label: MS spectra (input file) + - datatypes: + - tabular + label: Experimental design template + button_link: "{{ galaxy_base_url }}/root?tool_id=toolshed.g2.bx.psu.edu/repos/galaxyp/maxquant/maxquant/" + - title_md: "MSstats" + description_md: > +

+ Statistical relative protein significance analysis in DDA, SRM and DIA Mass Spectrometry. +

+ inputs: + - datatypes: + - tabular + - csv + label: Either the 10-column MSstats format or the outputs of spectral processing tools such as MaxQuant, OpenSWATH. + button_link: "{{ galaxy_base_url }}/root?tool_id=toolshed.g2.bx.psu.edu/repos/galaxyp/msstats/msstats/" + - title_md: "LFQ Analyst" + description_md: > +

+ Analyze and Visualize Label-Free Proteomics output from MaxQuant. +

+ inputs: + - datatypes: + - txt + label: Protein groups (MaxQuant output) + - datatypes: + - txt + label: Experimental Design Matrix (MaxQuant output) + button_link: "{{ galaxy_base_url }}/root?tool_id=interactive_tool_lfqanalyst_2" + - id: help + title: Help + content: + - title_md: "LFQ-Analyst: Manual" + description_md: > +

+ A detailed user manual for LFQ-Analyst. +

+ button_link: "https://analyst-suite.monash-proteomics.cloud.edu.au/apps/lfq-analyst/LFQ-Analyst_manual.pdf" + button_md: "Manual" + - title_md: "MaxQuant and MSstats for the analysis of label-free data" + description_md: > +

+ Learn how to use MaxQuant and MSstats for the analysis of label-free shotgun (DDA) data. +

+ button_link: "https://training.galaxyproject.org/training-material/topics/proteomics/tutorials/maxquant-msstats-dda-lfq/tutorial.html" + button_md: "Tutorial" + - title_md: "Galaxy Australia support" + description_md: > +

+ Any user of Galaxy Australia can request support through an online form. +

+ button_link: "/request/support" + button_md: "Request support" diff --git a/subdomains/proteomics/sections/dda_tmt.yml b/subdomains/proteomics/sections/dda_tmt.yml new file mode 100644 index 00000000..ae8bd0b8 --- /dev/null +++ b/subdomains/proteomics/sections/dda_tmt.yml @@ -0,0 +1,60 @@ +id: dda_tmt +title: DDA TMT +tabs: + - id: tools + title: Tools + heading_md: > + Some example tools are listed here: you can also search for more in the full tool panel to the left. + content: + - title_md: "MaxQuant" + description_md: > +

+ MaxQuant is a quantitative proteomics software package designed for analyzing large mass-spectrometric data sets. +

+ inputs: + - datatypes: + - thermo.raw + - mzML + - mzXML + label: MS spectra (input file) + - datatypes: + - tabular + label: Experimental design template + button_link: "{{ galaxy_base_url }}/root?tool_id=toolshed.g2.bx.psu.edu/repos/galaxyp/maxquant/maxquant/" + - title_md: "TMT Analyst" + description_md: > +

+ Analyze and Visualize Label-Free Proteomics output from MaxQuant. +

+ inputs: + - datatypes: + - txt + label: Protein groups (MaxQuant output) + - datatypes: + - txt + label: Experimental Design Matrix (MaxQuant output) + button_link: "{{ galaxy_base_url }}/root?tool_id=interactive_tool_tmtanalyst" + - id: help + title: Help + content: + - title_md: "TMT-Analyst: Manual" + description_md: > +

+ A detailed user manual for TMT-Analyst. +

+ button_link: "https://analyst-suites.org/apps/tmt-analyst/TMT-Analyst-manual.pdf" + button_md: "Manual" + - title_md: "MaxQuant and MSstats for the analysis of TMT data" + description_md: > +

+ Learn how to use MaxQuant and MSstats for the analysis of TMT labelled shotgun (DDA) data. +

+ button_link: "https://training.galaxyproject.org/training-material/topics/proteomics/tutorials/maxquant-msstats-tmt/tutorial.html" + button_md: "Tutorial" + - title_md: "Galaxy Australia support" + description_md: > +

+ Any user of Galaxy Australia can request support through an online form. +

+ button_link: "/request/support" + button_md: "Request support" diff --git a/subdomains/proteomics/sections/dia_standardised_tools.yml b/subdomains/proteomics/sections/dia_standardised_tools.yml new file mode 100644 index 00000000..825e07f5 --- /dev/null +++ b/subdomains/proteomics/sections/dia_standardised_tools.yml @@ -0,0 +1,43 @@ +id: dia_standardised_tools +title: DIA Standardised Tools +tabs: + - id: tools + title: Tools + heading_md: > + Some example tools are listed here: you can also search for more in the full tool panel to the left. + content: + - title_md: "MSstats" + description_md: > +

+ Statistical relative protein significance analysis in DDA, SRM and DIA Mass Spectrometry. +

+ inputs: + - datatypes: + - tabular + - csv + label: Either the 10-column MSstats format or the outputs of spectral processing tools such as MaxQuant, OpenSWATH. + button_link: "{{ galaxy_base_url }}/root?tool_id=toolshed.g2.bx.psu.edu/repos/galaxyp/msstats/msstats/" + - id: help + title: Help + content: + - title_md: "Galaxy Australia support" + description_md: > +

+ Any user of Galaxy Australia can request support through an online form. +

+ button_link: "/request/support" + button_md: "Request support" + - title_md: "DIA Analysis using OpenSwathWorkflow" + description_md: > +

+ Learn how to analyse HEK-Ecoli spike-in DIA data in Galaxy, understand DIA data principles and characteristics, and use OpenSwathworkflow to analyze HEK-Ecoli spike-in DIA data. +

+ button_link: "https://usegalaxy.org.au/training-material/topics/proteomics/tutorials/DIA_Analysis_OSW/tutorial.html" + button_md: "Tutorial" + - title_md: "Library Generation for DIA Analysis" + description_md: > +

+ Learn how to generate a spectral library from data dependent acquisition (DDA) MS data, understand DIA data principles and characteristics, and optimize and refine a spectral library for the analysis of DIA data. +

+ button_link: "https://usegalaxy.org.au/training-material/topics/proteomics/tutorials/DIA_lib_OSW/tutorial.html" + button_md: "Tutorial" diff --git a/subdomains/proteomics/static/custom.css b/subdomains/proteomics/static/custom.css new file mode 100644 index 00000000..cfd8f237 --- /dev/null +++ b/subdomains/proteomics/static/custom.css @@ -0,0 +1,21 @@ +#whatIsThisPage { + position: absolute; + top: 135px; + right: calc(50vw - 500px); +} + +@media (max-width: 1070px) { + #whatIsThisPage { + right: 2rem; + } +} +@media (max-width: 992px) { + #whatIsThisPage { + top: 95px; + } +} +@media (max-width: 730px) { + #whatIsThisPage { + top: 2rem; + } +} diff --git a/subdomains/proteomics/static/logo.png b/subdomains/proteomics/static/logo.png new file mode 100644 index 0000000000000000000000000000000000000000..f90d01be6851cb746c1116b459107eaff75bf95d GIT binary patch literal 2845 zcmeHJTU65d7XE`aF_xBDCp9l+B%O|-W-6p^RU-%?Y-A;?QiY%?Qid_ z6TV0E^-T2u0MJJr^*jjxpmePrfPANo%%7H0w9ohPM+0#Hux`^I2I?Ortpb1^7v<@3 zI=Oguv^$A^#v)S~Je6#l6oPGF`h%Y6{bJBz&}Hz;lkGFxf8S2mJM{fzJep0c3pp`N$L5N^`cjFP{Hb4N2a=`suYgEJ6T@!T7Z@83Ea0c1a|xA0(wRu za>tk5nbE_VuzlzrN-cY8a%u7+GaCe|a)|^3H?n|3p;o|&I}l*A^_SfZ@NJ;^b4|=Z zDDwF8W6v?Z&9)P{WpG2_>c3)W5fnf6)P4@;GaF)N0KSTCV;(dQ+bHt{cy$k}*RuBh zC9<#M{`Tzn`y{Nx^l+rpGJ-tUx{Uy|pwWCYSm3S|JB-GK^AtJT_A?SbURVTFUZU>+ zGB&WWEc!-J(7>miaXQ{sUv9e4kv4?5LAEkDH4VU4_{vpicg-)2B#Lqt7~_HE`)vt+S@(Kx4xS+uM1Sikk9W@e*t(jit&7Qn4OnvfW!q*h?vavhDw zS3JpL1WBYN_>ib%84fC%AN4rqOus823q7?Ix4<$WKCwL5BcySvYvs9FA!a9dB74HfegBpby!QH z&iQym^agDyFG>wG0M%1zyEWL`V$-~L7K>iwLlsNhKORJtNNjEI}1QkQfZax!WoQmLnHa~%$8l{7+2PVBJ?)kO(l7+Wj;Lop->3j8E-lZtsd)O{x zlX^oZ`-7z;vK^`>uWbMCbZ53Wr0-o1YB;uVUXkAzp#2p7&LcH$pqvzA0z!4^GLs6G zgU>75%kD$EhqW;VUpR+mBZ28uMCRQhm!ZS!=UAj)jg$Zg$)n?q? z@Q5cjGA5L3^0tF%_nu)bUlkZ7XP6mWmr;ie(pnXLnV;9k*qg%|hw6L!EZ;Mj+xYTHBdb20wBS#|ihLEMl}96sI}K&w;sR zGOGPzG{jr#j5%RQ>+K@e9#z%|BY#!6#ndl;;a4RR_L3&M=#VZvD@jq9e`=?6Ih&t1 z5AxqTz#z7oNaZt~wwYm#TBTdo%b{2(3BTsG%1D=!yJ|)%o?p9?+Q`f1%91%nEV0Zz z*+fnF#8MSX+(n|@sviC%wFSj)^3}+r2>Z|^;tS^xv`9VoVC&(SCMJ^f()6%kwv?ID zBUBRXT|fA1_cP&^x13^lHrQicoFi9|6tfG*#|lDA)w@*Ri`p|64QNulYY{v^;^(A6 z64!rZeu#QCkR#ut-R>!r_WW8jpC}ogdXUQH3@`T;u5+Y|)HEsOU|>X2rQdqN%E#A_ z7;e~u&&=Cy)j-RqJ6~PqzsbWOn&#oOnlYxO?4k0^wrv=Z>*Q!Scy^8C8l+;zi#frJnaIF^8{YP?tx! z5}isb#zG&XTxP$*B;^e)E zJEJHE5T|YJ|DjIe6_*Hv#TZAcHssKPAgyP1eZ&FRF=FQ%V(*xR0XXPA2CHoKE8}mQ z&MYIPbi=-^vQ<d-f;VK1lJGPp{{T${d zSEwu!48weg8wNGA3fvsu_zh7?)91F%4vM{2WlPhF&jhw3ThIof4pOs+a$oDRauH^_ zf1Yv6JRBINTmV6U)!zI5~vRgNnvWh!00gOjHr6-8j k+8VAIpVi93e{)H;hgFMigyQin+Its3dHH%){19^e-@=xq8vp + diff --git a/subdomains/proteomics/templates/intro.html b/subdomains/proteomics/templates/intro.html new file mode 100644 index 00000000..41a4c0d1 --- /dev/null +++ b/subdomains/proteomics/templates/intro.html @@ -0,0 +1,155 @@ +
+ + What is this page? + +

+ Welcome to the Galaxy Australia Proteomics Lab. Get quick access to the + tools, workflows and tutorials you need to get started with proteomics on + Galaxy. +

+ +

+ This page is currently under development in consultation with the + + Australian Proteomics Bioinformatics community. +

+
+ +
+ +
+
+
+ + + + + + +
+ school + + Proteomics tutorials for Galaxy +
+ +
+ +
+ + + + + + +
+ account_tree + + Shared proteomics histories +
+ +
+
+
+ +
+ + + + + + From ce1e50a8f73a6bb66a3096af48b610d810bd99eb Mon Sep 17 00:00:00 2001 From: Cameron Hyde Date: Thu, 29 Aug 2024 08:14:29 +1000 Subject: [PATCH 02/18] Add Genome lab --- subdomains/genome/CONTRIBUTORS | 3 + subdomains/genome/annotation.yml | 361 +++++++++++++++ subdomains/genome/assembly.yml | 411 ++++++++++++++++++ subdomains/genome/base.yml | 36 ++ subdomains/genome/data.yml | 160 +++++++ .../genome/static/annotation-overview.png | Bin 0 -> 129906 bytes .../genome/static/assembly-overview.png | Bin 0 -> 157857 bytes subdomains/genome/static/custom.css | 0 subdomains/genome/static/logo.png | Bin 0 -> 8296 bytes subdomains/genome/templates/conclusion.html | 163 +++++++ subdomains/genome/templates/footer.html | 10 + subdomains/genome/templates/intro.html | 40 ++ 12 files changed, 1184 insertions(+) create mode 100644 subdomains/genome/CONTRIBUTORS create mode 100644 subdomains/genome/annotation.yml create mode 100644 subdomains/genome/assembly.yml create mode 100644 subdomains/genome/base.yml create mode 100644 subdomains/genome/data.yml create mode 100644 subdomains/genome/static/annotation-overview.png create mode 100644 subdomains/genome/static/assembly-overview.png create mode 100644 subdomains/genome/static/custom.css create mode 100644 subdomains/genome/static/logo.png create mode 100644 subdomains/genome/templates/conclusion.html create mode 100644 subdomains/genome/templates/footer.html create mode 100644 subdomains/genome/templates/intro.html diff --git a/subdomains/genome/CONTRIBUTORS b/subdomains/genome/CONTRIBUTORS new file mode 100644 index 00000000..bb026f69 --- /dev/null +++ b/subdomains/genome/CONTRIBUTORS @@ -0,0 +1,3 @@ +# If GitHub username, name and avatar will be fetched and displayed +AnnaSyme +neoformit diff --git a/subdomains/genome/annotation.yml b/subdomains/genome/annotation.yml new file mode 100644 index 00000000..e693290a --- /dev/null +++ b/subdomains/genome/annotation.yml @@ -0,0 +1,361 @@ +id: annotation +title: Genome annotation +tabs: + - id: tools + title: Tools + heading_md: > + Common tools are listed here, or search for more in the full tool panel to the left. + content: + - title_md: MAKER - genome annotation pipeline + description_md: > +

+ MAKER is able to annotate both prokaryotes and eukaryotes. It works by aligning as many evidences as possible along the genome sequence, and then reconciling all these signals to determine probable gene structures. +

The evidences can be transcript or protein sequences from the same (or closely related) organism. These sequences can come from public databases (like NR or GenBank) or from your own experimental data (transcriptome assembly from an RNASeq experiment for example). MAKER is also able to take into account repeated elements. +

+ inputs: + - label: Genome assembly + datatypes: + - fasta + - label: Protein evidence (optional) + datatypes: + - fasta + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fmaker%2Fmaker" + - title_md: Funannotate predict - predicted gene annotations + description_md: > +

+ Funannotate predict performs a comprehensive whole genome gene prediction. Uses AUGUSTUS, GeneMark, Snap, GlimmerHMM, BUSCO, EVidence Modeler, tbl2asn, tRNAScan-SE, Exonerate, minimap2. This approach differs from Maker as it does not need to train ab initio predictors. +

+ inputs: + - datatypes: + - fasta + label: Genome assembly (soft-masked) + - datatypes: + - bam + label: Mapped RNA evidence (optional) + - datatypes: + - fasta + label: Protein evidence (optional) + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Ffunannotate_predict%2Ffunannotate_predict" + - title_md: RepeatMasker - screen DNA sequences for interspersed repeats and low complexity regions + description_md: > +

+ RepeatMasker is a program that screens DNA for repeated elements such as tandem repeats, transposons, SINEs and LINEs. Galaxy AU has installed the full and curated DFam screening databases, or a custom database can be provided in fasta format. Additional reference data can be downloaded from RepBase. +

+ inputs: + - datatypes: + - fasta + label: Genome assembly + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fbgruening%2Frepeat_masker%2Frepeatmasker_wrapper" + - title_md: InterProScan - Scans InterPro database and assigns functional annotations + description_md: > +

+ Interproscan is a batch tool to query the InterPro database. It provides annotations based on multiple searches of profile and other functional databases. +

+ inputs: + - datatypes: + - fasta + label: Genome assembly + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fbgruening%2Finterproscan%2Finterproscan" + - title_md: Funannotate compare - compare several annotations + description_md: > +

+ Funannotate compare compares several annotations and outputs a GFF3 file with the best gene models. It can be used to compare the results of different gene predictors, or to compare the results of a gene predictor with a reference annotation. +

+ inputs: + - datatypes: + - fasta + label: Genome assemblies to compare + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Ffunannotate_compare%2Ffunannotate_compare" + - title_md: JBrowse - Genome browser to visualize annotations + description_md: '' + inputs: + - datatypes: + - fasta + label: Genome assembly + - datatypes: + - gff + - gff3 + - bed + label: Annotations + - datatypes: + - bam + label: Mapped RNAseq data (optional) + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fjbrowse%2Fjbrowse" + - title_md: Prokka - Genome annotation, prokaryotes only + description_md: '' + inputs: + - datatypes: + - fasta + label: Genome assembly + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fcrs4%2Fprokka%2Fprokka" + + - title_md: FGenesH - Genome annotation + description_md: > +

+ Annotate an assembled genome and output a GFF3 file. There are several modules that do different things - search for FGENESH in the tool panel to see them. +

+

+ Note: you must + + apply for access + + to this tool before use. +

+ inputs: + - datatypes: + - fasta + label: Genome assembly + - datatypes: + - fasta + label: Repeat-masked (hard) genome assembly + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=fgenesh_annotate&version=latest" + + - id: workflows + title: Workflows + heading_md: > + A workflow is a series of Galaxy tools that have been linked together to perform a specific analysis. You can use and customize the example workflows below. + Learn more. + content: + subsections: + - id: general + title: General use + content: + - title_md: Annotation with Maker + description_md: > +

+ Annotates a genome using multiple rounds of Maker, including gene prediction using SNAP and Augustus.

Tools: maker snap augustus busco jbrowse +

+ inputs: + - label: Genome assembly + datatypes: + - fasta + - label: RNAseq Illumina reads + datatypes: + - fastq + - label: Proteins + datatypes: + - fasta + button_link: "{{ galaxy_base_url }}/u/anna/w/genome-annotation-with-maker" + view_link: '' + view_tip: '' + button_tip: Run in Galaxy AU + - title_md: Annotation with Funannotate + description_md: > +

+ Annotates a genome using Funannotate, includes RNAseq data with RNAstar, and protein predictions from EggNOG.

Tools: RNAstar funannotate eggnog busco jbrowse aegean parseval +

+ inputs: + - label: Genome assembly (soft-masked) + datatypes: + - fasta + - label: RNAseq Illumina reads + datatypes: + - fastq + - label: Alternative annotation + datatypes: + - gff3 + - label: Alternative annotation + datatypes: + - gbk + button_link: "{{ galaxy_base_url }}/u/anna/w/annotation-funannotate" + view_link: '' + view_tip: '' + button_tip: Run in Galaxy AU + + - id: tsi_transcripts + title: Transcript alignment + content: + - title_md: About these workflows + description_md: > +

+ This How-to-Guide will describe the steps required to align transcript data to your genome on the Galaxy Australia platform, using multiple workflows. The outputs from these workflows can then be used as inputs into the next annotation workflow using FgenesH++. +

+ - title_md: Repeat masking + description_md: > +

+ Mask repeats in the genome. +

+ inputs: + - datatypes: + - fasta + label: Assembled genome genome.fasta + button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=875" + view_link: https://workflowhub.eu/workflows/875 + view_tip: View in WorkflowHub + button_tip: Import to Galaxy Australia + + - title_md: QC and trimming of RNAseq + description_md: > +

+ Trim and merge RNAseq reads. +

+ inputs: + - datatypes: + - fastqsanger.gz + label: "For each tissue: RNAseq R1 files in a collection R1.fastqsanger.gz; RNAseq R2 files in a collection R2.fastqsanger.gz" + button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=876" + view_link: https://workflowhub.eu/workflows/876 + view_tip: View in WorkflowHub + button_tip: Import to Galaxy Australia + + - title_md: Find transcripts + description_md: > +

+ Align RNAseq to genome to find transcripts. +

+ inputs: + - datatypes: + - fasta + label: Masked genome masked_genome.fasta + - fastqsanger.gz + label: "For each tissue: Trimmed and merged RNAseq R1 files R1.fastqsanger.gz; Trimmed and merged RNAseq R2 files R2.fastqsanger.gz" + button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=877" + view_link: https://workflowhub.eu/workflows/877 + view_tip: View in WorkflowHub + button_tip: Import to Galaxy Australia + + - title_md: Combine transcripts + description_md: > +

+ Merge transcriptomes from different tissues, and filter out non-coding sequences. +

+ inputs: + - datatypes: + - fasta + label: Masked genome masked_genome.fasta + - gtf + label: Multiple transcriptomes in a collection transcriptome.gtf + - fasta.gz + label: Coding and non-coding sequences from NCBI coding_seqs.fna.gz non-coding_seqs.fna.gz + button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=878" + view_link: https://workflowhub.eu/workflows/878 + view_tip: View in WorkflowHub + button_tip: Import to Galaxy Australia + + - title_md: Extract transcripts + description_md: > +

+ Extract longest transcripts. +

+ inputs: + - datatypes: + - fasta + label: Merged transcriptomes merged_transcriptomes.fasta + button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=879" + view_link: https://workflowhub.eu/workflows/879 + view_tip: View in WorkflowHub + button_tip: Import to Galaxy Australia + + + - title_md: Convert formats + description_md: > +

+ Convert formats for FgenesH++ +

+ inputs: + - datatypes: + - fasta + label: Transdecoder nucleotides transdecoder_nucleotides.fasta + label: Transdecoder peptides transdecoder_peptides.fasta + button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=880" + view_link: https://workflowhub.eu/workflows/880 + view_tip: View in WorkflowHub + button_tip: Import to Galaxy Australia + + - id: tsi_annotation + title: Annotation with FgenesH++ + content: + - title_md: About these workflows + description_md: > +

+ This How-to-Guide will describe the steps required to annotate your genome on the Galaxy Australia platform, using multiple workflows. +

+ - title_md: Annotation with FgenesH++ + description_md: > + Annotate the genome using outputs from the TSI transcriptome workflows. +

+ Note: you must + + apply for access + + to this tool before use. +

+ inputs: + - datatypes: + - fasta + label: Assembled genome + - datatypes: + - fasta + label: Masked genome + - datatypes: + - fasta + label: > + Outputs from TSI convert formats workflow + (*.cdna, + *.pro, + *.dat) + button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=881" + view_link: https://workflowhub.eu/workflows/881 + view_tip: View in WorkflowHub + button_tip: Import to Galaxy Australia + + - id: help + title: Help + content: + - title_md: What is genome annotation? + description_md: > +

+ These slides from the Galaxy training network explain the process of genome annotation in detail. You can use the ← and → keys to navigate through the slides. +

+ - title_md: Genome annotation overview + description_md: > +

+ The flowchart below shows how you might use your input data (in green) with different Galaxy tools (in blue) to annotate a genome assembly. For example, one pathway would be taking an assembled genome, plus information about repeats, and data from RNA-seq, to run in the Maker pipeline. The annotatations can then be viewed in JBrowse. +

+ Genome annotation flowchart +

+ A graphical representation of genome annotation +

+ - title_md: Can I use Fgenesh++ for annotation? + description_md: > +

+ Fgenesh++ is a bioinformatics pipeline for automatic prediction of genes in eukaryotic genomes. It is presently not installed in Galaxy Australia, but the Australian Biocommons and partners have licensed the software and made it available via commandline. Australian researchers can apply for access through the Australian BioCommons. +

+ button_md: Apply + button_link: https://www.biocommons.org.au/fgenesh-plus-plus + button_tip: Apply for access to Fgenesh++ + - title_md: Can I use Apollo to share and edit the annotated genome? + description_md: > +

+ Apollo is web-browser accessible system that lets you conduct real-time collaborative curation and editing of genome annotations. +

+

+ The Australian BioCommons and our partners at QCIF and Pawsey provide a hosted Apollo Portal service where your genome assembly and supporting evidence files can be hosted. All system administration is taken care of, so you and your team can focus on the annotation curation itself. +

+

+ This Galaxy tutorial provides a complete walkthrough of the process of refining eukaryotic genome annotations with Apollo. +

+ button_md: More info + button_link: https://support.biocommons.org.au/support/solutions/articles/6000244843-apollo-for-collaborative-curation-and-editing + - title_md: Tutorials + description_md: > +

+ Genome annotation with Maker +

+

+ Genome annotation of eukaryotes is a little more complicated than for prokaryotes: eukaryotic genomes are usually larger than prokaryotes, with more genes. The sequences determining the beginning and the end of a gene are generally less conserved than the prokaryotic ones. Many genes also contain introns, and the limits of these introns (acceptor and donor sites) are not highly conserved. This Galaxy tutorial uses MAKER to annotate the genome of a small eukaryote: Schizosaccharomyces pombe (a yeast). +

+
+

+ Genome annotation with Funannotate +

+

+ This Galaxy tutorial provides a complete walkthrough of the process of annotation with Funannotate, including the preparation of RNAseq data, structural annotation, functional annotation, visualisation, and comparing annotations. +

+ - title_md: Galaxy Australia support + description_md: > +

+ Any user of Galaxy Australia can request support through an online form. +

+ button_md: Request support + button_link: /request/support diff --git a/subdomains/genome/assembly.yml b/subdomains/genome/assembly.yml new file mode 100644 index 00000000..7609262f --- /dev/null +++ b/subdomains/genome/assembly.yml @@ -0,0 +1,411 @@ +id: assembly +title: Genome assembly +tabs: + - id: tools + title: Tools + heading_md: > + Common tools are listed here, or search for more in the full tool panel to the left. + content: + - title_md: Hifiasm - assembly with PacBio HiFi data + description_md: > +

+ A haplotype-resolved assembler for PacBio HiFi reads. +

+ inputs: + - datatypes: + - fasta + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fbgruening%2Fhifiasm%2Fhifiasm" + - title_md: Flye - assembly with PacBio or Nanopore data + description_md: > +

+ de novo assembly of single-molecule sequencing reads, designed for a wide range of datasets, from small bacterial projects to large mammalian-scale assemblies. +

+ inputs: + - datatypes: + - fasta + - fastq + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fbgruening%2Fflye%2Fflye" + - title_md: Unicycler - assembly with Illumina, PacBio or Nanopore data - bacteria only + description_md: > +

+ Hybrid assembly pipeline for bacterial genomes, uses both Illumina reads and long reads (PacBio or Nanopore). +

+ inputs: + - datatypes: + - fastq + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Funicycler%2Funicycler" + - title_md: YAHS - scaffold assembly with HiC data + description_md: > +

+ YAHS is a scaffolding tool based on a computational method that exploits the genomic proximity information in Hi-C data sets for long-range scaffolding of de novo genome assemblies. Inputs are the primary assembly (or haplotype 1), and HiC reads mapped to the assembly. See this tutorial to learn how to create a suitable BAM file. +

+ inputs: + - label: Primary assembly or Haplotype 1 genome.fasta + datatypes: + - fasta + - label: HiC reads mapped to assembly mapped_reads.bam + datatypes: + - bam + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2yahs" + - title_md: Quast - assess genome assembly quality + description_md: > +

+ QUAST = QUality ASsessment Tool. The tool evaluates genome assemblies by computing various metrics. If you have one or multiple genome assemblies, you can assess their quality with Quast. It works with or without reference genome. +

+ inputs: + - datatypes: + - fasta + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fquast%2Fquast" + - title_md: Busco - assess genome assembly quality + description_md: > +

+ BUSCO: assessing genome assembly and annotation completeness with Benchmarking Universal Single-Copy Orthologs. The tool attempts to provide a quantitative assessment of the completeness in terms of the expected gene content of a genome assembly, transcriptome, or annotated gene set. +

+ inputs: + - datatypes: + - fasta + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fbusco%2Fbusco" + - title_md: MitoHiFi - assemble mitochondrial genomes + description_md: > +

+ Assemble mitochondrial genomes from PacBio HiFi reads. Run first to find a related mitogenome, then run to assemble the genome. Inputs are PacBio HiFi reads in fasta or fastq format, and a related mitogenome in both fasta and genbank formats. +

+ inputs: + - datatypes: + - fasta + - fastq + - genbank + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fbgruening%2Fmitohifi%2Fmitohfi" + + - id: workflows + title: Workflows + heading_md: > + A workflow is a series of Galaxy tools that have been linked together to perform a specific analysis. You can use and customize the example workflows below. + Learn more. + content: + subsections: + - id: pacbio + title: TSI assembly workflows - PacBio HiFi data + content: + - title_md: About these workflows + description_md: > +

+ This How-to-Guide will describe the steps required to assemble your genome on the Galaxy Australia platform, using multiple workflows. There is also a guide about the Genome Assessment workflow, and the HiC Scaffolding workflow. +

+ - title_md: BAM to FASTQ + QC v1.0 + description_md: > +

+ Convert a BAM file to FASTQ format to perform QC analysis (required if your data is in BAM format). +

+ inputs: + - datatypes: + - bam + label: PacBio subreads.bam + button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=220" + view_link: https://workflowhub.eu/workflows/220 + view_tip: View in WorkflowHub + button_tip: Import to Galaxy Australia + - title_md: PacBio HiFi genome assembly using hifiasm v2.1 + description_md: > +

+ Assemble a genome using PacBio HiFi reads. +

+ inputs: + - datatypes: + - fastqsanger + label: HiFi reads + button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=221" + view_link: https://workflowhub.eu/workflows/221 + view_tip: View in WorkflowHub + button_tip: Import to Galaxy Australia + - title_md: Purge duplicates from hifiasm assembly v1.0 + description_md: > +

+ Optional workflow to purge duplicates from the contig assembly. +

+ inputs: + - datatypes: + - fastqsanger + label: HiFi reads + - datatypes: + - fasta + label: Primary assembly contigs + button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=237" + view_link: https://workflowhub.eu/workflows/237 + view_tip: View in WorkflowHub + button_tip: Import to Galaxy Australia + - title_md: Genome assessment post-assembly + description_md: > +

+ Evaluate the quality of your genome assembly with a comprehensive report including FASTA stats, BUSCO, QUAST, Meryl and Merqury. +

+ inputs: + - datatypes: + - fasta + label: Primary assembly contigs + button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=403" + view_link: https://workflowhub.eu/workflows/403 + view_tip: View in WorkflowHub + button_tip: Import to Galaxy Australia + - title_md: Optional HiC scaffolding workflow + description_md: > +

+ If you have HiC data, scaffold your assembly using YAHS. +

+ inputs: + - datatypes: + - fasta + label: Primary or Hap1 assembly + - datatypes: + - fastqsanger.gz + label: HiC forward reads, HiC reverse reads + button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=1054" + view_link: https://workflowhub.eu/workflows/1054 + view_tip: View in WorkflowHub + button_tip: Import to Galaxy Australia + + - id: nanopore + title: General assembly workflows - Nanopore and Illumina data + content: + - title_md: About these workflows + description_md: > +

+ This tutorial describes the steps required to assemble a genome on Galaxy with Nanopore and Illumina data. +

+ - title_md: Flye assembly with Nanopore data + description_md: > +

+ Assemble Nanopore long reads. This workflow can be run alone or as part of a combined workflow for large genome assembly. +

+ inputs: + - datatypes: + - fastqsanger + label: Long reads (may be raw, filtered and/or corrected) + button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=225" + view_link: https://workflowhub.eu/workflows/225 + view_tip: View in WorkflowHub + button_tip: Import to Galaxy Australia + - title_md: Assembly polishing + description_md: > +

+ Polishes (corrects) an assembly, using long reads (Racon and Medaka) and short reads (Racon). +

+ inputs: + - datatypes: + - fasta + label: Assembly to polish + - datatypes: + - fastq + label: Long reads (those used in assembly) + - datatypes: + - fastq + label: Short reads to be used for polishing (R1 only) + button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=226" + view_link: https://workflowhub.eu/workflows/226 + view_tip: View in WorkflowHub + button_tip: Import to Galaxy Australia + - title_md: Assess genome quality + description_md: > +

+ Assesses the quality of the genome assembly. Generates statistics, determines if expected genes are present and align contigs to a reference genome. +

+ inputs: + - datatypes: + - fasta + label: Polished assembly + - datatypes: + - fasta + label: Reference genome assembly (e.g. related species) + button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=229" + view_link: https://workflowhub.eu/workflows/229 + view_tip: View in WorkflowHub + button_tip: Import to Galaxy Australia + - id: hic + title: VGP assembly workflows - PacBio HiFi and (optional) HiC data + content: + - title_md: About these workflows + description_md: > +

+ These workflows have been developed as part of the global Vertebrate Genome Project (VGP). A guide to using these in Galaxy Australia can be found here. A complete guide to the individual workflows and sample results can be found here. There are many different ways that these workflows can be used in practice - for a comprehensive example, check out this Galaxy tutorial. +

+ - title_md: Kmer profiling + description_md: > +

+ This workflow produces a Meryl database and Genomescope outputs that will be used to determine parameters for following workflows, and assess the quality of genome assemblies. Specifically, it provides information about the genomic complexity, such as the genome size and levels of heterozygosity and repeat content, as well about the data quality. +

+ inputs: + - datatypes: + - fastq + label: PacBio HiFi reads + button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=dockstore.org&trs_id=%23workflow/github.com/iwc-workflows/kmer-profiling-hifi-VGP1/main" + view_link: https://dockstore.org/workflows/github.com/iwc-workflows/kmer-profiling-hifi-VGP1/main:main + view_tip: View in WorkflowHub + button_tip: Import to Galaxy Australia + - title_md: Hifi assembly and HiC phasing + description_md: > +

+ This workflow uses hifiasm (HiC mode) to generate HiC-phased haplotypes (hap1 and hap2). This is in contrast to its default mode, which generates primary and alternate pseudohaplotype assemblies. This workflow includes three tools for evaluating assembly quality: gfastats, BUSCO and Merqury. +

+

+ Note: if you have multiple input files for each HiC set, they need to be concatenated. The forward set needs to be concatenated in the same order as reverse set. +

+ inputs: + - datatypes: + - fasta + label: PacBio HiFi reads + - datatypes: + - fastq + label: PacBio HiC reads (forward) + - datatypes: + - fastq + label: PacBio HiC reads (reverse) + - datatypes: + - meryldb + label: Meryl kmer database + - datatypes: + - txt + label: GenomeScope genome profile summary + button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=dockstore.org&trs_id=%23workflow/github.com/iwc-workflows/Assembly-Hifi-HiC-phasing-VGP4/main" + view_link: https://dockstore.org/workflows/github.com/iwc-workflows/Assembly-Hifi-HiC-phasing-VGP4/main:main + view_tip: View in WorkflowHub + button_tip: Import to Galaxy Australia + + - title_md: Hifi assembly without HiC data + description_md: > +

+ This workflow uses hifiasm to generate primary and alternate pseudohaplotype assemblies. This workflow includes three tools for evaluating assembly quality: gfastats, BUSCO and Merqury. +

+ inputs: + - datatypes: + - fasta + label: PacBio HiFi reads + - datatypes: + - meryldb + label: Meryl kmer database + - datatypes: + - txt + label: GenomeScope genome profile summary + button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=dockstore.org&trs_id=%23workflow/github.com/iwc-workflows/Assembly-Hifi-only-VGP3/main" + view_link: https://dockstore.org/workflows/github.com/iwc-workflows/Assembly-Hifi-only-VGP3/main:main + view_tip: View in Dockstore + button_tip: Import to Galaxy Australia + + - title_md: HiC scaffolding + description_md: > +

+ This workflow scaffolds the assembly contigs using information from HiC data. +

+ inputs: + - datatypes: + - gfa + label: Assembly of haplotype 1 + - datatypes: + - fastq + label: HiC forward reads + - datatypes: + - fastq + label: HiC reverse reads + button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=dockstore.org&trs_id=%23workflow/github.com/iwc-workflows/Scaffolding-HiC-VGP8/main" + view_link: https://dockstore.org/workflows/github.com/iwc-workflows/Scaffolding-HiC-VGP8/main:main + view_tip: View in WorkflowHub + button_tip: Import to Galaxy Australia + - title_md: Decontamination + description_md: > +

+ This workflow identifies and removes contaminants from the assembly. +

+ inputs: + - datatypes: + - fasta + label: Assembly + button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=dockstore.org&trs_id=%23workflow/github.com/iwc-workflows/Assembly-decontamination-VGP9/main:v0.1" + view_link: https://dockstore.org/workflows/github.com/iwc-workflows/Assembly-decontamination-VGP9/main:v0.1 + view_tip: View in WorkflowHub + button_tip: Import to Galaxy Australia + + - id: help + title: Help + content: + - title_md: Can I use Galaxy Australia to assemble a large genome? + description_md: > +

+ Yes. Galaxy Australia has assembly tools for small prokaryote genomes as well as larger eukaryote genomes. We are continually adding new tools and optimising them for large genome assemblies - this means adding enough computer processing power to run data-intensive tools, as well as configuring aspects such as parallelisation. +

+

+ Please contact us if: +

+
    +
  • you need to increase your data storage limit
    • +
  • there is a tool you wish to request
    • +
  • a tool appears to be broken or running slowly
    • +
+ button_md: Request support + button_link: /request + - title_md: How can I learn about genome assembly? + description_md: > +
    +
  • See the tutorials in this Help section. They cover different approaches to genome assembly.
    • +
  • Read the methods in scientific papers about genome assembly, particularly those about genomes with similar characteristics to those in your project
    • +
  • See the Workflows section for examples of different approaches to genome assembly - these cover different sequencing data types, and a variety of tools.
    • +
+ - title_md: Genome assembly overview + description_md: > +

+ Genome assembly can be a very involved process. A typical genome assembly procedure might look like: +

+
    +
  • Data QC - check the quality and characteristics of your sequencing reads.
    • +
  • Kmer counting - to determine genome characteristics such as ploidy and size.
    • +
  • Data preparation - trimming and filtering sequencing reads if required.
    • +
  • Assembly - for large genomes, this is usually done with long sequencing reads from PacBio or Nanopore.
    • +
  • Polishing - the assembly may be polished (corrected) with long and/or short (Illumina) reads.
    • +
  • Scaffolding - the assembly contigs may be joined together with other sequencing data such as HiC.
    • +
  • Assessment - at any stage, the assembly can be assessed for number of contigs, number of base pairs, whether expected genes are present, and many other metrics.
    • +
  • Annotation - identify features on the genome assembly such as gene names and locations.
    • +
+ Genome assembly flowchart +

+ A graphical representation of genome assembly +

+ - title_md: Which tools should I use? + description_md: > +

+ There is no best set of tools to recommend - new tools are developed constantly, sequencing technology improves rapidly, and many genomes have never been sequenced before and thus their characteristics and quirks are unknown. The "Tools" tab in this section includes a list of commonly-used tools that could be a good starting point. You will find other tools in recent publications or used in workflows. +

+

+ You can also search for tools in Galaxy's tool panel. If they aren't installed on Galaxy Australia, you can request installation of a tool. +

+

+ We recommend testing a tool on a small data set first and seeing if the results make sense, before running on your full data set. +

+ - title_md: Tutorials + description_md: > +

+ Find 15+ Galaxy training tutorials here. +

+

+ Introduction to genome assembly and annotation (slides) +

+

+ Vertebrate genome assembly pipeline (tutorial) +

+

+ Nanopore and illumina genome assembly (tutorial) +

+

+ Share workflows and results with workflow reports (tutorial) +

+ - title_md: How can I assess the quality of my genome assembly? + description_md: > +

+ Once a genome has been assembled, it is important to assess the quality of the assembly, and in the first instance, this quality control (QC) can be achieved using the workflow described here. +

+ button_md: Workflow tutorial + button_link: https://australianbiocommons.github.io/how-to-guides/genome_assembly/assembly_qc + - title_md: Galaxy Australia support + description_md: > +

+ Any user of Galaxy Australia can request support through an online form. +

+ button_md: Request support + button_link: /request/support diff --git a/subdomains/genome/base.yml b/subdomains/genome/base.yml new file mode 100644 index 00000000..232de7a9 --- /dev/null +++ b/subdomains/genome/base.yml @@ -0,0 +1,36 @@ +# Test this locally with: +# http://127.0.0.1:8000/lab/export?content_root=http://localhost:8000/static/home/labs/genome/base.yml + +# Request this on site.usegalaxy.org.au with: +# https://site.usegalaxy.org.au/lab/export?content_root=https://site.usegalaxy.org.au/static/home/labs/genome/base.yml + +# Check out the documentation for building exported labs: +# https://site.usegalaxy.org.au/lab/export + +# Use these variables in HTML templates like: +# "Welcome to the Galaxy {{ site_name }} {{ lab_name }}" +# To make the content more generic and reusable across sites +site_name: Australia +lab_name: Genome Lab +nationality: Australian +galaxy_base_url: https://genome.usegalaxy.org.au # Use for rendering tool/workflow URLs. Trailing '/' will be removed. +subdomain: genome +root_domain: usegalaxy.org.au +feedback_email: help@genome.edu.au + +# Custom content relative to this file URL +header_logo: static/logo.png +custom_css: static/custom.css +intro_md: templates/intro.html +conclusion_md: templates/conclusion.html +footer_md: templates/footer.html + + +# Data (Tools, Workflows etc.) to be rendered into sections/tabs/accordion elements. +# Either: +# 1. Relative to this file URL +# 2. Full URL to fetch globally centralized content +sections: + - data.yml + - assembly.yml + - annotation.yml diff --git a/subdomains/genome/data.yml b/subdomains/genome/data.yml new file mode 100644 index 00000000..79ba2c2b --- /dev/null +++ b/subdomains/genome/data.yml @@ -0,0 +1,160 @@ +id: data +title: Data import and preparation +tabs: + - id: tools + title: Tools + heading_md: > + Common tools are listed here, or search for more in the full tool panel to the left. + content: + - title_md: Import data to Galaxy + description_md: > + Standard upload of data to Galaxy, from your computer or from the web. + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=upload1" + - title_md: FastQC - sequence quality reports + description_md: > +

+ Before using your sequencing data, it's important to ensure that + the data quality is sufficient for your analysis. +

+ inputs: + - datatypes: + - fastq + - bam + - sam + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Ffastqc%2Ffastqc" + - title_md: FastP - sequence quality reports, trimming & filtering + description_md: > +

+ Faster run than FastQC, this tool can also trim reads and filter by quality. +

+ inputs: + - datatypes: + - fastq + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Ffastp%2Ffastp" + - title_md: NanoPlot - visualize Oxford Nanopore data + description_md: > +

+ A plotting suite for Oxford Nanopore sequencing data and alignments. +

+ inputs: + - datatypes: + - fastq + - fasta + - vcf_bgzip + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fnanoplot%2Fnanoplot" + - title_md: GenomeScope - estimate genome size + description_md: > +

+ A set of metrics and graphs to visualize genome size and complexity prior to assembly. +

+ inputs: + - datatypes: + - tabular + label: Output from Meryl or Jellyfish histo + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fgenomescope%2Fgenomescope" + - title_md: Meryl - count kmers + description_md: > +

+ Prepare kmer count histogram for input to GenomeScope. +

+ inputs: + - datatypes: + - fastq + - fasta + button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fmeryl%2Fmeryl" + - id: workflows + title: Workflows + heading_md: > + A workflow is a series of Galaxy tools that have been linked together to perform a specific analysis. You can use and customize the example workflows below. + Learn more. + content: + - title_md: Data QC + description_md: > +

+ Report statistics from sequencing reads.

Tools: nanoplot fastqc multiqc +

+ button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=222" + view_link: https://workflowhub.eu/workflows/222 + view_tip: View in WorkflowHub + button_tip: Import to Galaxy AU + - title_md: Kmer counting to estimate genome size + description_md: > +

+ Estimates genome size and heterozygosity based on counts of kmers.

Tools: meryl genomescope +

+ button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=223" + view_link: https://workflowhub.eu/workflows/223 + view_tip: View in WorkflowHub + button_tip: Import to Galaxy AU + - title_md: Trim and filter reads + description_md: > +

+ Trims and filters raw sequence reads according to specified settings.

Tools: fastp +

+ button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=224" + view_link: https://workflowhub.eu/workflows/224 + view_tip: View in WorkflowHub + button_tip: Import to Galaxy AU + - id: help + title: Help + content: + - title_md: How can I import my genomics data? + description_md: > +

+ You can upload your data to Galaxy using the Upload tool from anywhere in Galaxy. Just look for the "Upload data" button at the top of the tool panel. +

+ button_md: More info + button_link: https://training.galaxyproject.org/training-material/topics/galaxy-interface/ + - title_md: How can I subsample my data? + description_md: > +

+ We recommend subsampling large data sets to test tools and workflows. A useful tool is seqtk_seq, setting the parameter at "Sample fraction of sequences". +

+ - title_md: How can I import data from the BPA portal? + description_md: > +

+ BioPlatforms Australia allows data downloads via URL. Once you have generated one of these URLs in the BPA portal, you can import it into Galaxy using the "Fetch data" feature of the Upload tool. +

+ button_md: More info + button_link: https://australianbiocommons.github.io/how-to-guides/genome_assembly/hifi_assembly#in-depth-workflow-guide + - title_md: Can I upload sensitive data? + description_md: > +

+ No, do not upload personal or sensitive, such as human health or clinical data. Please see our Data Privacy page for definitions of sensitive and health-related information. +

+

+ Please also make sure you have read our Terms of Service, which covers hosting and analysis of research data. +

+ - title_md: Is my data private? + description_md: > +

+ Please read our Privacy Policy for information on your personal data and any data that you upload. +

+ - title_md: How can I increase my storage quota? + description_md: > +

+ Please submit a quota request if your Galaxy Australia account reaches its data storage limit. Requests are usually provisioned quickly if you provide a reasonable use case for your request. +

+ button_md: Request + button_link: /request/quota + - title_md: "Tutorial: Quality Control" + description_md: > +

+ Quality control and data cleaning is an essential first step in any NGS analysis. This tutorial will show you how to use and interpret results from FastQC, NanoPlot and PycoQC. +

+ button_md: Tutorial + button_link: https://training.galaxyproject.org/training-material/topics/sequence-analysis/tutorials/quality-control/tutorial.html + - title_md: "Tutorial: introduction to Genomics and Galaxy" + description_md: > +

+ This practical aims to familiarize you with the Galaxy user interface. It will teach you how to perform basic tasks such as importing data, running tools, working with histories, creating workflows, and sharing your work. +

+ button_md: Tutorial + button_link: https://training.galaxyproject.org/training-material/topics/introduction/tutorials/galaxy-intro-strands/tutorial.html + - title_md: Galaxy Australia support + description_md: > +

+ Any user of Galaxy Australia can request support through an online form. +

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+

What's happening in {{ nationality }} genomics research?

+ +
+
+

What do you think of the {{ lab_name }}?

+ +
+ + + + + + + + diff --git a/subdomains/genome/templates/footer.html b/subdomains/genome/templates/footer.html new file mode 100644 index 00000000..172d3c74 --- /dev/null +++ b/subdomains/genome/templates/footer.html @@ -0,0 +1,10 @@ + + diff --git a/subdomains/genome/templates/intro.html b/subdomains/genome/templates/intro.html new file mode 100644 index 00000000..e2ea1c74 --- /dev/null +++ b/subdomains/genome/templates/intro.html @@ -0,0 +1,40 @@ +
+

+ Welcome to the Galaxy {{ site_name }} {{ lab_name }}. Get quick access to tools, workflows and tutorials for genome assembly and annotation. +
+ + What is this page? + +

+ + +
From 6831cc0d675700f326e2d20818fcadf4811c1cd3 Mon Sep 17 00:00:00 2001 From: Cameron Hyde Date: Thu, 29 Aug 2024 15:40:39 +1000 Subject: [PATCH 03/18] Fix up genome lab --- subdomains/assets/site-switcher.png | Bin 0 -> 12090 bytes subdomains/genome/base.yml | 17 +- subdomains/genome/templates/conclusion.html | 163 ------------------ subdomains/genome/templates/footer.html | 10 -- subdomains/genome/templates/intro.html | 4 +- subdomains/genome/usegalaxy.org.au.yml | 22 +++ .../templates/conclusion.html | 163 ++++++++++++++++++ .../usegalaxy.org.au/templates/footer.html | 10 ++ .../usegalaxy.org.au/templates/intro.html | 40 +++++ 9 files changed, 244 insertions(+), 185 deletions(-) create mode 100644 subdomains/assets/site-switcher.png create mode 100644 subdomains/genome/usegalaxy.org.au.yml create mode 100644 subdomains/genome/usegalaxy.org.au/templates/conclusion.html create mode 100644 subdomains/genome/usegalaxy.org.au/templates/footer.html create mode 100644 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site.usegalaxy.org.au with: -# https://site.usegalaxy.org.au/lab/export?content_root=https://site.usegalaxy.org.au/static/home/labs/genome/base.yml +# https://site.usegalaxy.org.au/lab/export?content_root=https://github.com/galaxyproject/galaxy_codex/blob/main/subdomains/genome/base.yml # Check out the documentation for building exported labs: # https://site.usegalaxy.org.au/lab/export @@ -10,13 +7,13 @@ # Use these variables in HTML templates like: # "Welcome to the Galaxy {{ site_name }} {{ lab_name }}" # To make the content more generic and reusable across sites -site_name: Australia +site_name: "" lab_name: Genome Lab -nationality: Australian -galaxy_base_url: https://genome.usegalaxy.org.au # Use for rendering tool/workflow URLs. Trailing '/' will be removed. +nationality: "" +galaxy_base_url: https://genome.usegalaxy.org # Use for rendering tool/workflow URLs. Trailing '/' will be removed. subdomain: genome -root_domain: usegalaxy.org.au -feedback_email: help@genome.edu.au +root_domain: usegalaxy.org +# feedback_email: help@mygalaxy.org # set to enable feedback form # Custom content relative to this file URL header_logo: static/logo.png @@ -29,7 +26,7 @@ footer_md: templates/footer.html # Data (Tools, Workflows etc.) to be rendered into sections/tabs/accordion elements. # Either: # 1. Relative to this file URL -# 2. Full URL to fetch globally centralized content +# 2. Full URL to fetch content from a different remote location sections: - data.yml - assembly.yml diff --git a/subdomains/genome/templates/conclusion.html b/subdomains/genome/templates/conclusion.html index 13e7d771..e69de29b 100644 --- a/subdomains/genome/templates/conclusion.html +++ b/subdomains/genome/templates/conclusion.html @@ -1,163 +0,0 @@ -
-

What's happening in {{ nationality }} genomics research?

- -
-
-

What do you think of the {{ lab_name }}?

- -
- - - - - - - - diff --git a/subdomains/genome/templates/footer.html b/subdomains/genome/templates/footer.html index 172d3c74..e69de29b 100644 --- a/subdomains/genome/templates/footer.html +++ b/subdomains/genome/templates/footer.html @@ -1,10 +0,0 @@ - - diff --git a/subdomains/genome/templates/intro.html b/subdomains/genome/templates/intro.html index e2ea1c74..67c8467a 100644 --- a/subdomains/genome/templates/intro.html +++ b/subdomains/genome/templates/intro.html @@ -23,8 +23,8 @@
Galaxy {{ site_name }}

- Switch between sites quickly using the dropdown in Galaxy {{ site_name }}'s main navigation bar: - Switch site button + Switch between sites quickly using the dropdown in Galaxy {{ site_name }}'s main navigation bar (note, if your Galaxy server does not have this, please request that they install the "Site Switcher" plugin from Galaxy Australia): + Switch site button

Take me back to Galaxy {{ site_name }}

diff --git a/subdomains/genome/usegalaxy.org.au.yml b/subdomains/genome/usegalaxy.org.au.yml new file mode 100644 index 00000000..a47dea03 --- /dev/null +++ b/subdomains/genome/usegalaxy.org.au.yml @@ -0,0 +1,22 @@ +# This content extends base.yml + +# Request this on site.usegalaxy.org.au with: +# https://site.usegalaxy.org.au/lab/export?content_root=https://github.com/galaxyproject/galaxy_codex/blob/main/subdomains/genome/usegalaxy.org.au.yml + +# Check out the documentation for building exported labs: +# https://site.usegalaxy.org.au/lab/export + +# Use these variables in HTML templates like: +# "Welcome to the Galaxy {{ site_name }} {{ lab_name }}" +# To make the content more generic and reusable across sites +site_name: Australia +lab_name: Genome Lab +nationality: Australian +galaxy_base_url: https://genome.usegalaxy.org.au # Use for rendering tool/workflow URLs. Trailing '/' will be removed. +subdomain: genome +root_domain: usegalaxy.org.au +feedback_email: help@genome.edu.au + +# Custom content relative to this file URL +conclusion_md: usegalaxy.org.au/templates/conclusion.html +footer_md: usegalaxy.org.au/templates/footer.html diff --git a/subdomains/genome/usegalaxy.org.au/templates/conclusion.html b/subdomains/genome/usegalaxy.org.au/templates/conclusion.html new file mode 100644 index 00000000..13e7d771 --- /dev/null +++ b/subdomains/genome/usegalaxy.org.au/templates/conclusion.html @@ -0,0 +1,163 @@ +
+

What's happening in {{ nationality }} genomics research?

+ +
+
+

What do you think of the {{ lab_name }}?

+ +
+ + + + + + + + diff --git a/subdomains/genome/usegalaxy.org.au/templates/footer.html b/subdomains/genome/usegalaxy.org.au/templates/footer.html new file mode 100644 index 00000000..172d3c74 --- /dev/null +++ b/subdomains/genome/usegalaxy.org.au/templates/footer.html @@ -0,0 +1,10 @@ + + diff --git a/subdomains/genome/usegalaxy.org.au/templates/intro.html b/subdomains/genome/usegalaxy.org.au/templates/intro.html new file mode 100644 index 00000000..e2ea1c74 --- /dev/null +++ b/subdomains/genome/usegalaxy.org.au/templates/intro.html @@ -0,0 +1,40 @@ +
+

+ Welcome to the Galaxy {{ site_name }} {{ lab_name }}. Get quick access to tools, workflows and tutorials for genome assembly and annotation. +
+ + What is this page? + +

+ + +
From 67a53dfaf16fd3259b6e3059388a96bcfa8f4fd6 Mon Sep 17 00:00:00 2001 From: Cameron Hyde Date: Thu, 29 Aug 2024 15:40:49 +1000 Subject: [PATCH 04/18] Fix up proteomics lab --- subdomains/proteomics/base.yml | 31 +++++++++++------- .../proteomics/templates/conclusion.html | 1 - subdomains/proteomics/templates/footer.html | 9 ------ subdomains/proteomics/templates/intro.html | 30 +++++++---------- subdomains/proteomics/usegalaxy.org.au.yml | 32 +++++++++++++++++++ .../templates/conclusion.html | 1 + .../usegalaxy.org.au/templates/footer.html | 9 ++++++ 7 files changed, 73 insertions(+), 40 deletions(-) create mode 100644 subdomains/proteomics/usegalaxy.org.au.yml create mode 100644 subdomains/proteomics/usegalaxy.org.au/templates/conclusion.html create mode 100644 subdomains/proteomics/usegalaxy.org.au/templates/footer.html diff --git a/subdomains/proteomics/base.yml b/subdomains/proteomics/base.yml index 14625c6d..eb32af5a 100644 --- a/subdomains/proteomics/base.yml +++ b/subdomains/proteomics/base.yml @@ -1,22 +1,19 @@ -# Test this locally with: -# http://127.0.0.1:8000/lab/export?content_root=http://localhost:8000/static/home/labs/proteomics/base.yml - # Request this on site.usegalaxy.org.au with: -# https://site.usegalaxy.org.au/lab/export?content_root=https://site.usegalaxy.org.au/static/home/labs/proteomics/base.yml +# https://site.usegalaxy.org.au/lab/export?content_root=https://github.com/galaxyproject/galaxy_codex/blob/main/subdomains/proteomics/base.yml # Check out the documentation for building exported labs: # https://site.usegalaxy.org.au/lab/export # Use these variables in HTML templates like: # "Welcome to the Galaxy {{ site_name }} {{ lab_name }}" -# To make the content more generic and reusable across sites -site_name: Australia +# To make the content more generic and reusable across sites. +site_name: "" lab_name: Proteomics Lab -nationality: Australian -galaxy_base_url: https://proteomics.usegalaxy.org.au # Use for rendering tool/workflow URLs. Trailing '/' will be removed. +nationality: "" +galaxy_base_url: https://usegalaxy.org # Use for rendering tool/workflow URLs. Trailing '/' will be removed. subdomain: proteomics -root_domain: usegalaxy.org.au -feedback_email: help@genome.edu.au +root_domain: usegalaxy.org +# feedback_email: help@mygalaxy.org # Set to enable feedback form # Custom content relative to this file URL header_logo: static/logo.png @@ -28,7 +25,7 @@ footer_md: templates/footer.html # Data (Tools, Workflows etc.) to be rendered into sections/tabs/accordion elements. # Either: # 1. Relative to this file URL -# 2. Full URL to fetch globally centralized content +# 2. Full URL to fetch content from a different remote location sections: - sections/data.yml - sections/conversion_modification.yml @@ -36,3 +33,15 @@ sections: - sections/dda_standardised_tools.yml - sections/dia_standardised_tools.yml - sections/dda_tmt.yml + +# Histories listed here will be shown in a table +# An empty list will remove this feature from the page +shared_histories: + - url: https://usegalaxy.org.au/u/mike/h/lfq-and-tmt + label_html: > + Demo data for LFQanalyst and TMTanalyst. + - url: https://usegalaxy.org.au/u/johan/h/maxquant-and-msstats-for-the-analysis-of-label-free-data-1 + label_html: > + Example data provided for the "MaxQuant and MSstats for the analysis of + label-free data" Galaxy Training Network (GTN) tutorial, which is available + here. diff --git a/subdomains/proteomics/templates/conclusion.html b/subdomains/proteomics/templates/conclusion.html index c097223b..e69de29b 100644 --- a/subdomains/proteomics/templates/conclusion.html +++ b/subdomains/proteomics/templates/conclusion.html @@ -1 +0,0 @@ -{% include 'home/snippets/header-cards.html' %} diff --git a/subdomains/proteomics/templates/footer.html b/subdomains/proteomics/templates/footer.html index 259d18e6..e69de29b 100644 --- a/subdomains/proteomics/templates/footer.html +++ b/subdomains/proteomics/templates/footer.html @@ -1,9 +0,0 @@ - - diff --git a/subdomains/proteomics/templates/intro.html b/subdomains/proteomics/templates/intro.html index 41a4c0d1..396cd894 100644 --- a/subdomains/proteomics/templates/intro.html +++ b/subdomains/proteomics/templates/intro.html @@ -8,7 +8,7 @@ What is this page?

- Welcome to the Galaxy Australia Proteomics Lab. Get quick access to the + Welcome to the {{ site_name }} {{ lab_name }}. Get quick access to the tools, workflows and tutorials you need to get started with proteomics on Galaxy.

@@ -39,6 +39,7 @@ + {% if shared_histories %}
+ {% endif %} @@ -91,12 +93,14 @@
Galaxy {{ site_name }} {{ lab_name }}
and {{ lab_name }} ({{ subdomain }}.{{ root_domain }}) is easy - look for the icon in the top navigation bar to switch between sites: - Switch site button + Switch site button

+ {% if site_name == 'Australia' %}

Please note: unfortunately, AAF login is not configured for Galaxy Labs, but we are working on fixing this.

+ {% endif %}

Take me back to the Base site

@@ -117,34 +121,22 @@
Galaxy {{ site_name }} Shared Histories

- This set of proteomics-specific shared histories contain test or demo data for software available on Proteomics Lab. You can copy these histories directly to your account on Galaxy, making it faster to get started! + This set of proteomics-specific shared histories contain test or demo data for software available on {{ lab_name }}. You can copy these histories directly to your account on Galaxy, making it faster to get started!

+ {% for history in shared_histories %} - - - - + {% endif %}
- Demo data for LFQanalyst and TMTanalyst. + {{ history.label_html|safe }} - - View history - -
- Example data provided for the "MaxQuant and MSstats for the analysis of label-free data" Galaxy Training Network (GTN) tutorial, which is available - here. - - + View history
diff --git a/subdomains/proteomics/usegalaxy.org.au.yml b/subdomains/proteomics/usegalaxy.org.au.yml new file mode 100644 index 00000000..d6922c65 --- /dev/null +++ b/subdomains/proteomics/usegalaxy.org.au.yml @@ -0,0 +1,32 @@ +# This content extends base.yml + +# Request this on site.usegalaxy.org.au with: +# https://site.usegalaxy.org.au/lab/export?content_root=https://github.com/galaxyproject/galaxy_codex/blob/main/subdomains/proteomics/usegalaxy.org.au.yml + +# Check out the documentation for building exported labs: +# https://site.usegalaxy.org.au/lab/export + +# Use these variables in HTML templates like: +# "Welcome to the Galaxy {{ site_name }} {{ lab_name }}" +# To make the content more generic and reusable across sites +site_name: Australia +lab_name: Proteomics Lab +nationality: Australian +galaxy_base_url: https://proteomics.usegalaxy.org.au # Use for rendering tool/workflow URLs. Trailing '/' will be removed. +subdomain: proteomics +root_domain: usegalaxy.org.au +feedback_email: help@genome.edu.au + +# Custom content relative to this file URL +conclusion_md: usegalaxy.org.au/templates/conclusion.html +footer_md: usegalaxy.org.au/templates/footer.html + +shared_histories: + - url: https://usegalaxy.org.au/u/mike/h/lfq-and-tmt + label_html: > + Demo data for LFQanalyst and TMTanalyst. + - url: https://usegalaxy.org.au/u/johan/h/maxquant-and-msstats-for-the-analysis-of-label-free-data-1 + label_html: > + Example data provided for the "MaxQuant and MSstats for the analysis of + label-free data" Galaxy Training Network (GTN) tutorial, which is available + here. \ No newline at end of file diff --git a/subdomains/proteomics/usegalaxy.org.au/templates/conclusion.html b/subdomains/proteomics/usegalaxy.org.au/templates/conclusion.html new file mode 100644 index 00000000..c097223b --- /dev/null +++ b/subdomains/proteomics/usegalaxy.org.au/templates/conclusion.html @@ -0,0 +1 @@ +{% include 'home/snippets/header-cards.html' %} diff --git a/subdomains/proteomics/usegalaxy.org.au/templates/footer.html b/subdomains/proteomics/usegalaxy.org.au/templates/footer.html new file mode 100644 index 00000000..259d18e6 --- /dev/null +++ b/subdomains/proteomics/usegalaxy.org.au/templates/footer.html @@ -0,0 +1,9 @@ + + From a66347e1c4fa1ce6c27cd8f6bbdb80fd4bf0d9a0 Mon Sep 17 00:00:00 2001 From: Cameron Hyde Date: Thu, 29 Aug 2024 15:41:29 +1000 Subject: [PATCH 05/18] Edit comment line in singlecell/base.yml --- subdomains/singlecell/base.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subdomains/singlecell/base.yml b/subdomains/singlecell/base.yml index cb34d2c6..8fd77d1b 100644 --- a/subdomains/singlecell/base.yml +++ b/subdomains/singlecell/base.yml @@ -59,7 +59,7 @@ footer_md: templates/footer.html # Data (Tools, Workflows etc.) to be rendered into sections/tabs/accordion elements. # Either: # 1. Relative to this file URL -# 2. Full URL to fetch globally centralized content +# 2. Full URL to fetch content from a different remote location sections: - sections/1_beginner.yml - sections/3_advanced.yml From c1372f4e04d80aab01596cedd741bd5c58248903 Mon Sep 17 00:00:00 2001 From: Cameron Hyde Date: Thu, 29 Aug 2024 15:46:13 +1000 Subject: [PATCH 06/18] Fix for-loop closing tag --- subdomains/proteomics/templates/intro.html | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subdomains/proteomics/templates/intro.html b/subdomains/proteomics/templates/intro.html index 396cd894..89e30a5d 100644 --- a/subdomains/proteomics/templates/intro.html +++ b/subdomains/proteomics/templates/intro.html @@ -136,7 +136,7 @@
Galaxy {{ site_name }} Shared Histories
- {% endif %} + {% endfor %}
From cd006e3fcc4f2c1ef5cd2027ce1340fa8da596fe Mon Sep 17 00:00:00 2001 From: Cameron Hyde Date: Thu, 5 Sep 2024 11:52:25 +1000 Subject: [PATCH 07/18] Reword genome>data --- subdomains/genome/data.yml | 130 +++++++++++++++++++------------------ 1 file changed, 67 insertions(+), 63 deletions(-) diff --git a/subdomains/genome/data.yml b/subdomains/genome/data.yml index 79ba2c2b..76254e1e 100644 --- a/subdomains/genome/data.yml +++ b/subdomains/genome/data.yml @@ -1,6 +1,73 @@ id: data title: Data import and preparation tabs: + - id: help + title: Overview + content: + header_md: > + If you are new to galaxy, uploading your data is a good place to start! +
+ Check out the Tools and Workflows tabs for different approaches to uploading data. + - title_md: How can I import my genomics data? + description_md: > +

+ You can upload your data to Galaxy using the Upload tool from anywhere in Galaxy. Just look for the "Upload data" button at the top of the tool panel. +

+ button_md: More info + button_link: https://training.galaxyproject.org/training-material/topics/galaxy-interface/ + - title_md: How can I subsample my data? + description_md: > +

+ We recommend subsampling large data sets to test tools and workflows. A useful tool is seqtk_seq, setting the parameter at "Sample fraction of sequences". +

+ - title_md: How can I import data from the BPA portal? + description_md: > +

+ BioPlatforms Australia allows data downloads via URL. Once you have generated one of these URLs in the BPA portal, you can import it into Galaxy using the "Fetch data" feature of the Upload tool. +

+ button_md: More info + button_link: https://australianbiocommons.github.io/how-to-guides/genome_assembly/hifi_assembly#in-depth-workflow-guide + - title_md: Can I upload sensitive data? + description_md: > +

+ No, do not upload personal or sensitive, such as human health or clinical data. Please see our Data Privacy page for definitions of sensitive and health-related information. +

+

+ Please also make sure you have read our Terms of Service, which covers hosting and analysis of research data. +

+ - title_md: Is my data private? + description_md: > +

+ Please read our Privacy Policy for information on your personal data and any data that you upload. +

+ - title_md: How can I increase my storage quota? + description_md: > +

+ Please submit a quota request if your Galaxy Australia account reaches its data storage limit. Requests are usually provisioned quickly if you provide a reasonable use case for your request. +

+ button_md: Request + button_link: /request/quota + - title_md: "Tutorial: Quality Control" + description_md: > +

+ Quality control and data cleaning is an essential first step in any NGS analysis. This tutorial will show you how to use and interpret results from FastQC, NanoPlot and PycoQC. +

+ button_md: Tutorial + button_link: https://training.galaxyproject.org/training-material/topics/sequence-analysis/tutorials/quality-control/tutorial.html + - title_md: "Tutorial: introduction to Genomics and Galaxy" + description_md: > +

+ This practical aims to familiarize you with the Galaxy user interface. It will teach you how to perform basic tasks such as importing data, running tools, working with histories, creating workflows, and sharing your work. +

+ button_md: Tutorial + button_link: https://training.galaxyproject.org/training-material/topics/introduction/tutorials/galaxy-intro-strands/tutorial.html + - title_md: Galaxy Australia support + description_md: > +

+ Any user of Galaxy Australia can request support through an online form. +

+ button_md: Request support + button_link: /request/support - id: tools title: Tools heading_md: > @@ -95,66 +162,3 @@ tabs: view_link: https://workflowhub.eu/workflows/224 view_tip: View in WorkflowHub button_tip: Import to Galaxy AU - - id: help - title: Help - content: - - title_md: How can I import my genomics data? - description_md: > -

- You can upload your data to Galaxy using the Upload tool from anywhere in Galaxy. Just look for the "Upload data" button at the top of the tool panel. -

- button_md: More info - button_link: https://training.galaxyproject.org/training-material/topics/galaxy-interface/ - - title_md: How can I subsample my data? - description_md: > -

- We recommend subsampling large data sets to test tools and workflows. A useful tool is seqtk_seq, setting the parameter at "Sample fraction of sequences". -

- - title_md: How can I import data from the BPA portal? - description_md: > -

- BioPlatforms Australia allows data downloads via URL. Once you have generated one of these URLs in the BPA portal, you can import it into Galaxy using the "Fetch data" feature of the Upload tool. -

- button_md: More info - button_link: https://australianbiocommons.github.io/how-to-guides/genome_assembly/hifi_assembly#in-depth-workflow-guide - - title_md: Can I upload sensitive data? - description_md: > -

- No, do not upload personal or sensitive, such as human health or clinical data. Please see our Data Privacy page for definitions of sensitive and health-related information. -

-

- Please also make sure you have read our Terms of Service, which covers hosting and analysis of research data. -

- - title_md: Is my data private? - description_md: > -

- Please read our Privacy Policy for information on your personal data and any data that you upload. -

- - title_md: How can I increase my storage quota? - description_md: > -

- Please submit a quota request if your Galaxy Australia account reaches its data storage limit. Requests are usually provisioned quickly if you provide a reasonable use case for your request. -

- button_md: Request - button_link: /request/quota - - title_md: "Tutorial: Quality Control" - description_md: > -

- Quality control and data cleaning is an essential first step in any NGS analysis. This tutorial will show you how to use and interpret results from FastQC, NanoPlot and PycoQC. -

- button_md: Tutorial - button_link: https://training.galaxyproject.org/training-material/topics/sequence-analysis/tutorials/quality-control/tutorial.html - - title_md: "Tutorial: introduction to Genomics and Galaxy" - description_md: > -

- This practical aims to familiarize you with the Galaxy user interface. It will teach you how to perform basic tasks such as importing data, running tools, working with histories, creating workflows, and sharing your work. -

- button_md: Tutorial - button_link: https://training.galaxyproject.org/training-material/topics/introduction/tutorials/galaxy-intro-strands/tutorial.html - - title_md: Galaxy Australia support - description_md: > -

- Any user of Galaxy Australia can request support through an online form. -

- button_md: Request support - button_link: /request/support From f76a72c8a3464ed62c8cc082c665172dadab3ec8 Mon Sep 17 00:00:00 2001 From: Cameron Hyde Date: Thu, 5 Sep 2024 11:55:03 +1000 Subject: [PATCH 08/18] Fix syntax --- subdomains/genome/data.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subdomains/genome/data.yml b/subdomains/genome/data.yml index 76254e1e..ed2f6d36 100644 --- a/subdomains/genome/data.yml +++ b/subdomains/genome/data.yml @@ -3,11 +3,11 @@ title: Data import and preparation tabs: - id: help title: Overview - content: header_md: > If you are new to galaxy, uploading your data is a good place to start!
Check out the Tools and Workflows tabs for different approaches to uploading data. + content: - title_md: How can I import my genomics data? description_md: >

From 9712f3dab994d69250bf28fa01f9f6b038299c0b Mon Sep 17 00:00:00 2001 From: Cameron Hyde Date: Thu, 5 Sep 2024 12:13:18 +1000 Subject: [PATCH 09/18] Fix syntax --- subdomains/genome/data.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subdomains/genome/data.yml b/subdomains/genome/data.yml index ed2f6d36..52e551ed 100644 --- a/subdomains/genome/data.yml +++ b/subdomains/genome/data.yml @@ -8,7 +8,7 @@ tabs:
Check out the Tools and Workflows tabs for different approaches to uploading data. content: - - title_md: How can I import my genomics data? + - title_md: How can I import my genomics data? description_md: >

You can upload your data to Galaxy using the Upload tool from anywhere in Galaxy. Just look for the "Upload data" button at the top of the tool panel. From df10e572918e15e6fb252e79a4caa1d3129704a5 Mon Sep 17 00:00:00 2001 From: Cameron Hyde Date: Thu, 5 Sep 2024 12:22:00 +1000 Subject: [PATCH 10/18] Fix heading key --- subdomains/genome/data.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subdomains/genome/data.yml b/subdomains/genome/data.yml index 52e551ed..7ffbc0f7 100644 --- a/subdomains/genome/data.yml +++ b/subdomains/genome/data.yml @@ -3,7 +3,7 @@ title: Data import and preparation tabs: - id: help title: Overview - header_md: > + heading_md: > If you are new to galaxy, uploading your data is a good place to start!
Check out the Tools and Workflows tabs for different approaches to uploading data. From 592ad3ed752c88afced84b1a84c8bba5d8f10e76 Mon Sep 17 00:00:00 2001 From: Cameron Hyde Date: Mon, 9 Sep 2024 15:36:20 +1000 Subject: [PATCH 11/18] Add assembly workflow item --- subdomains/genome/assembly.yml | 12 ++++++++++++ 1 file changed, 12 insertions(+) diff --git a/subdomains/genome/assembly.yml b/subdomains/genome/assembly.yml index 7609262f..08b86a28 100644 --- a/subdomains/genome/assembly.yml +++ b/subdomains/genome/assembly.yml @@ -134,6 +134,18 @@ tabs: view_link: https://workflowhub.eu/workflows/237 view_tip: View in WorkflowHub button_tip: Import to Galaxy Australia + - title_md: Nanopore genome assembly using Flye + description_md: > + Assemble a genome using Nanopore reads. + inputs: + - datatypes: + - fastqsanger + - fastq + label: Nanopore reads + button_link: "{{ galaxy_base_url }}/workflows/trs_import?trs_server=workflowhub.eu&run_form=true&trs_id=1114" + view_link: https://workflowhub.eu/workflows/1114 + view_tip: View in WorkflowHub + button_tip: Import to Galaxy Australia - title_md: Genome assessment post-assembly description_md: >

From 9d0c990e97c6f27b184b698651e3f09433d52191 Mon Sep 17 00:00:00 2001 From: Cameron Hyde Date: Mon, 9 Sep 2024 15:36:30 +1000 Subject: [PATCH 12/18] Remove redundant

tags --- subdomains/genome/assembly.yml | 56 +++------------------------------- 1 file changed, 4 insertions(+), 52 deletions(-) diff --git a/subdomains/genome/assembly.yml b/subdomains/genome/assembly.yml index 08b86a28..2fb323b8 100644 --- a/subdomains/genome/assembly.yml +++ b/subdomains/genome/assembly.yml @@ -8,18 +8,14 @@ tabs: content: - title_md: Hifiasm - assembly with PacBio HiFi data description_md: > -

A haplotype-resolved assembler for PacBio HiFi reads. -

inputs: - datatypes: - fasta button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fbgruening%2Fhifiasm%2Fhifiasm" - title_md: Flye - assembly with PacBio or Nanopore data description_md: > -

de novo assembly of single-molecule sequencing reads, designed for a wide range of datasets, from small bacterial projects to large mammalian-scale assemblies. -

inputs: - datatypes: - fasta @@ -27,18 +23,14 @@ tabs: button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fbgruening%2Fflye%2Fflye" - title_md: Unicycler - assembly with Illumina, PacBio or Nanopore data - bacteria only description_md: > -

Hybrid assembly pipeline for bacterial genomes, uses both Illumina reads and long reads (PacBio or Nanopore). -

inputs: - datatypes: - fastq button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Funicycler%2Funicycler" - title_md: YAHS - scaffold assembly with HiC data description_md: > -

YAHS is a scaffolding tool based on a computational method that exploits the genomic proximity information in Hi-C data sets for long-range scaffolding of de novo genome assemblies. Inputs are the primary assembly (or haplotype 1), and HiC reads mapped to the assembly. See this tutorial to learn how to create a suitable BAM file. -

inputs: - label: Primary assembly or Haplotype 1 genome.fasta datatypes: @@ -49,32 +41,26 @@ tabs: button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2yahs" - title_md: Quast - assess genome assembly quality description_md: > -

QUAST = QUality ASsessment Tool. The tool evaluates genome assemblies by computing various metrics. If you have one or multiple genome assemblies, you can assess their quality with Quast. It works with or without reference genome. -

inputs: - datatypes: - fasta button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fquast%2Fquast" - title_md: Busco - assess genome assembly quality description_md: > -

BUSCO: assessing genome assembly and annotation completeness with Benchmarking Universal Single-Copy Orthologs. The tool attempts to provide a quantitative assessment of the completeness in terms of the expected gene content of a genome assembly, transcriptome, or annotated gene set. -

inputs: - datatypes: - fasta button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fbusco%2Fbusco" - title_md: MitoHiFi - assemble mitochondrial genomes description_md: > -

Assemble mitochondrial genomes from PacBio HiFi reads. Run first to find a related mitogenome, then run to assemble the genome. Inputs are PacBio HiFi reads in fasta or fastq format, and a related mitogenome in both fasta and genbank formats. -

inputs: - datatypes: - - fasta - - fastq - - genbank + - fasta + - fastq + - genbank button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fbgruening%2Fmitohifi%2Fmitohfi" - id: workflows @@ -85,18 +71,14 @@ tabs: content: subsections: - id: pacbio - title: TSI assembly workflows - PacBio HiFi data + title: TSI assembly workflows - PacBio HiFi or Nanopore data content: - title_md: About these workflows description_md: > -

This How-to-Guide will describe the steps required to assemble your genome on the Galaxy Australia platform, using multiple workflows. There is also a guide about the Genome Assessment workflow, and the HiC Scaffolding workflow. -

- title_md: BAM to FASTQ + QC v1.0 description_md: > -

Convert a BAM file to FASTQ format to perform QC analysis (required if your data is in BAM format). -

inputs: - datatypes: - bam @@ -107,9 +89,7 @@ tabs: button_tip: Import to Galaxy Australia - title_md: PacBio HiFi genome assembly using hifiasm v2.1 description_md: > -

Assemble a genome using PacBio HiFi reads. -

inputs: - datatypes: - fastqsanger @@ -120,9 +100,7 @@ tabs: button_tip: Import to Galaxy Australia - title_md: Purge duplicates from hifiasm assembly v1.0 description_md: > -

Optional workflow to purge duplicates from the contig assembly. -

inputs: - datatypes: - fastqsanger @@ -148,9 +126,7 @@ tabs: button_tip: Import to Galaxy Australia - title_md: Genome assessment post-assembly description_md: > -

Evaluate the quality of your genome assembly with a comprehensive report including FASTA stats, BUSCO, QUAST, Meryl and Merqury. -

inputs: - datatypes: - fasta @@ -161,9 +137,7 @@ tabs: button_tip: Import to Galaxy Australia - title_md: Optional HiC scaffolding workflow description_md: > -

If you have HiC data, scaffold your assembly using YAHS. -

inputs: - datatypes: - fasta @@ -181,14 +155,10 @@ tabs: content: - title_md: About these workflows description_md: > -

This tutorial describes the steps required to assemble a genome on Galaxy with Nanopore and Illumina data. -

- title_md: Flye assembly with Nanopore data description_md: > -

Assemble Nanopore long reads. This workflow can be run alone or as part of a combined workflow for large genome assembly. -

inputs: - datatypes: - fastqsanger @@ -199,9 +169,7 @@ tabs: button_tip: Import to Galaxy Australia - title_md: Assembly polishing description_md: > -

Polishes (corrects) an assembly, using long reads (Racon and Medaka) and short reads (Racon). -

inputs: - datatypes: - fasta @@ -218,9 +186,7 @@ tabs: button_tip: Import to Galaxy Australia - title_md: Assess genome quality description_md: > -

Assesses the quality of the genome assembly. Generates statistics, determines if expected genes are present and align contigs to a reference genome. -

inputs: - datatypes: - fasta @@ -237,14 +203,10 @@ tabs: content: - title_md: About these workflows description_md: > -

These workflows have been developed as part of the global Vertebrate Genome Project (VGP). A guide to using these in Galaxy Australia can be found here. A complete guide to the individual workflows and sample results can be found here. There are many different ways that these workflows can be used in practice - for a comprehensive example, check out this Galaxy tutorial. -

- title_md: Kmer profiling description_md: > -

This workflow produces a Meryl database and Genomescope outputs that will be used to determine parameters for following workflows, and assess the quality of genome assemblies. Specifically, it provides information about the genomic complexity, such as the genome size and levels of heterozygosity and repeat content, as well about the data quality. -

inputs: - datatypes: - fastq @@ -284,9 +246,7 @@ tabs: - title_md: Hifi assembly without HiC data description_md: > -

This workflow uses hifiasm to generate primary and alternate pseudohaplotype assemblies. This workflow includes three tools for evaluating assembly quality: gfastats, BUSCO and Merqury. -

inputs: - datatypes: - fasta @@ -304,9 +264,7 @@ tabs: - title_md: HiC scaffolding description_md: > -

This workflow scaffolds the assembly contigs using information from HiC data. -

inputs: - datatypes: - gfa @@ -323,9 +281,7 @@ tabs: button_tip: Import to Galaxy Australia - title_md: Decontamination description_md: > -

This workflow identifies and removes contaminants from the assembly. -

inputs: - datatypes: - fasta @@ -409,15 +365,11 @@ tabs:

- title_md: How can I assess the quality of my genome assembly? description_md: > -

Once a genome has been assembled, it is important to assess the quality of the assembly, and in the first instance, this quality control (QC) can be achieved using the workflow described here. -

button_md: Workflow tutorial button_link: https://australianbiocommons.github.io/how-to-guides/genome_assembly/assembly_qc - title_md: Galaxy Australia support description_md: > -

Any user of Galaxy Australia can request support through an online form. -

button_md: Request support button_link: /request/support From cfc531d178a8a28f847251a5187fbeaeeb606ca8 Mon Sep 17 00:00:00 2001 From: Cameron Hyde Date: Mon, 9 Sep 2024 15:49:26 +1000 Subject: [PATCH 13/18] Format YAML --- subdomains/genome/assembly.yml | 56 +++++++++++++++++----------------- 1 file changed, 28 insertions(+), 28 deletions(-) diff --git a/subdomains/genome/assembly.yml b/subdomains/genome/assembly.yml index 2fb323b8..fcd13631 100644 --- a/subdomains/genome/assembly.yml +++ b/subdomains/genome/assembly.yml @@ -8,14 +8,14 @@ tabs: content: - title_md: Hifiasm - assembly with PacBio HiFi data description_md: > - A haplotype-resolved assembler for PacBio HiFi reads. + A haplotype-resolved assembler for PacBio HiFi reads. inputs: - datatypes: - fasta button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fbgruening%2Fhifiasm%2Fhifiasm" - title_md: Flye - assembly with PacBio or Nanopore data description_md: > - de novo assembly of single-molecule sequencing reads, designed for a wide range of datasets, from small bacterial projects to large mammalian-scale assemblies. + de novo assembly of single-molecule sequencing reads, designed for a wide range of datasets, from small bacterial projects to large mammalian-scale assemblies. inputs: - datatypes: - fasta @@ -23,14 +23,14 @@ tabs: button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fbgruening%2Fflye%2Fflye" - title_md: Unicycler - assembly with Illumina, PacBio or Nanopore data - bacteria only description_md: > - Hybrid assembly pipeline for bacterial genomes, uses both Illumina reads and long reads (PacBio or Nanopore). + Hybrid assembly pipeline for bacterial genomes, uses both Illumina reads and long reads (PacBio or Nanopore). inputs: - datatypes: - fastq button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Funicycler%2Funicycler" - title_md: YAHS - scaffold assembly with HiC data description_md: > - YAHS is a scaffolding tool based on a computational method that exploits the genomic proximity information in Hi-C data sets for long-range scaffolding of de novo genome assemblies. Inputs are the primary assembly (or haplotype 1), and HiC reads mapped to the assembly. See this tutorial to learn how to create a suitable BAM file. + YAHS is a scaffolding tool based on a computational method that exploits the genomic proximity information in Hi-C data sets for long-range scaffolding of de novo genome assemblies. Inputs are the primary assembly (or haplotype 1), and HiC reads mapped to the assembly. See this tutorial to learn how to create a suitable BAM file. inputs: - label: Primary assembly or Haplotype 1 genome.fasta datatypes: @@ -41,26 +41,26 @@ tabs: button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2yahs" - title_md: Quast - assess genome assembly quality description_md: > - QUAST = QUality ASsessment Tool. The tool evaluates genome assemblies by computing various metrics. If you have one or multiple genome assemblies, you can assess their quality with Quast. It works with or without reference genome. + QUAST = QUality ASsessment Tool. The tool evaluates genome assemblies by computing various metrics. If you have one or multiple genome assemblies, you can assess their quality with Quast. It works with or without reference genome. inputs: - datatypes: - fasta button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fquast%2Fquast" - title_md: Busco - assess genome assembly quality description_md: > - BUSCO: assessing genome assembly and annotation completeness with Benchmarking Universal Single-Copy Orthologs. The tool attempts to provide a quantitative assessment of the completeness in terms of the expected gene content of a genome assembly, transcriptome, or annotated gene set. + BUSCO: assessing genome assembly and annotation completeness with Benchmarking Universal Single-Copy Orthologs. The tool attempts to provide a quantitative assessment of the completeness in terms of the expected gene content of a genome assembly, transcriptome, or annotated gene set. inputs: - datatypes: - fasta button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fbusco%2Fbusco" - title_md: MitoHiFi - assemble mitochondrial genomes description_md: > - Assemble mitochondrial genomes from PacBio HiFi reads. Run first to find a related mitogenome, then run to assemble the genome. Inputs are PacBio HiFi reads in fasta or fastq format, and a related mitogenome in both fasta and genbank formats. + Assemble mitochondrial genomes from PacBio HiFi reads. Run first to find a related mitogenome, then run to assemble the genome. Inputs are PacBio HiFi reads in fasta or fastq format, and a related mitogenome in both fasta and genbank formats. inputs: - datatypes: - - fasta - - fastq - - genbank + - fasta + - fastq + - genbank button_link: "{{ galaxy_base_url }}/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fbgruening%2Fmitohifi%2Fmitohfi" - id: workflows @@ -75,10 +75,10 @@ tabs: content: - title_md: About these workflows description_md: > - This How-to-Guide will describe the steps required to assemble your genome on the Galaxy Australia platform, using multiple workflows. There is also a guide about the Genome Assessment workflow, and the HiC Scaffolding workflow. + This How-to-Guide will describe the steps required to assemble your genome on the Galaxy Australia platform, using multiple workflows. There is also a guide about the Genome Assessment workflow, and the HiC Scaffolding workflow. - title_md: BAM to FASTQ + QC v1.0 description_md: > - Convert a BAM file to FASTQ format to perform QC analysis (required if your data is in BAM format). + Convert a BAM file to FASTQ format to perform QC analysis (required if your data is in BAM format). inputs: - datatypes: - bam @@ -89,7 +89,7 @@ tabs: button_tip: Import to Galaxy Australia - title_md: PacBio HiFi genome assembly using hifiasm v2.1 description_md: > - Assemble a genome using PacBio HiFi reads. + Assemble a genome using PacBio HiFi reads. inputs: - datatypes: - fastqsanger @@ -100,7 +100,7 @@ tabs: button_tip: Import to Galaxy Australia - title_md: Purge duplicates from hifiasm assembly v1.0 description_md: > - Optional workflow to purge duplicates from the contig assembly. + Optional workflow to purge duplicates from the contig assembly. inputs: - datatypes: - fastqsanger @@ -114,7 +114,7 @@ tabs: button_tip: Import to Galaxy Australia - title_md: Nanopore genome assembly using Flye description_md: > - Assemble a genome using Nanopore reads. + Assemble a genome using Nanopore reads. inputs: - datatypes: - fastqsanger @@ -126,7 +126,7 @@ tabs: button_tip: Import to Galaxy Australia - title_md: Genome assessment post-assembly description_md: > - Evaluate the quality of your genome assembly with a comprehensive report including FASTA stats, BUSCO, QUAST, Meryl and Merqury. + Evaluate the quality of your genome assembly with a comprehensive report including FASTA stats, BUSCO, QUAST, Meryl and Merqury. inputs: - datatypes: - fasta @@ -137,7 +137,7 @@ tabs: button_tip: Import to Galaxy Australia - title_md: Optional HiC scaffolding workflow description_md: > - If you have HiC data, scaffold your assembly using YAHS. + If you have HiC data, scaffold your assembly using YAHS. inputs: - datatypes: - fasta @@ -155,10 +155,10 @@ tabs: content: - title_md: About these workflows description_md: > - This tutorial describes the steps required to assemble a genome on Galaxy with Nanopore and Illumina data. + This tutorial describes the steps required to assemble a genome on Galaxy with Nanopore and Illumina data. - title_md: Flye assembly with Nanopore data description_md: > - Assemble Nanopore long reads. This workflow can be run alone or as part of a combined workflow for large genome assembly. + Assemble Nanopore long reads. This workflow can be run alone or as part of a combined workflow for large genome assembly. inputs: - datatypes: - fastqsanger @@ -169,7 +169,7 @@ tabs: button_tip: Import to Galaxy Australia - title_md: Assembly polishing description_md: > - Polishes (corrects) an assembly, using long reads (Racon and Medaka) and short reads (Racon). + Polishes (corrects) an assembly, using long reads (Racon and Medaka) and short reads (Racon). inputs: - datatypes: - fasta @@ -186,7 +186,7 @@ tabs: button_tip: Import to Galaxy Australia - title_md: Assess genome quality description_md: > - Assesses the quality of the genome assembly. Generates statistics, determines if expected genes are present and align contigs to a reference genome. + Assesses the quality of the genome assembly. Generates statistics, determines if expected genes are present and align contigs to a reference genome. inputs: - datatypes: - fasta @@ -203,10 +203,10 @@ tabs: content: - title_md: About these workflows description_md: > - These workflows have been developed as part of the global Vertebrate Genome Project (VGP). A guide to using these in Galaxy Australia can be found here. A complete guide to the individual workflows and sample results can be found here. There are many different ways that these workflows can be used in practice - for a comprehensive example, check out this Galaxy tutorial. + These workflows have been developed as part of the global Vertebrate Genome Project (VGP). A guide to using these in Galaxy Australia can be found here. A complete guide to the individual workflows and sample results can be found here. There are many different ways that these workflows can be used in practice - for a comprehensive example, check out this Galaxy tutorial. - title_md: Kmer profiling description_md: > - This workflow produces a Meryl database and Genomescope outputs that will be used to determine parameters for following workflows, and assess the quality of genome assemblies. Specifically, it provides information about the genomic complexity, such as the genome size and levels of heterozygosity and repeat content, as well about the data quality. + This workflow produces a Meryl database and Genomescope outputs that will be used to determine parameters for following workflows, and assess the quality of genome assemblies. Specifically, it provides information about the genomic complexity, such as the genome size and levels of heterozygosity and repeat content, as well about the data quality. inputs: - datatypes: - fastq @@ -246,7 +246,7 @@ tabs: - title_md: Hifi assembly without HiC data description_md: > - This workflow uses hifiasm to generate primary and alternate pseudohaplotype assemblies. This workflow includes three tools for evaluating assembly quality: gfastats, BUSCO and Merqury. + This workflow uses hifiasm to generate primary and alternate pseudohaplotype assemblies. This workflow includes three tools for evaluating assembly quality: gfastats, BUSCO and Merqury. inputs: - datatypes: - fasta @@ -264,7 +264,7 @@ tabs: - title_md: HiC scaffolding description_md: > - This workflow scaffolds the assembly contigs using information from HiC data. + This workflow scaffolds the assembly contigs using information from HiC data. inputs: - datatypes: - gfa @@ -281,7 +281,7 @@ tabs: button_tip: Import to Galaxy Australia - title_md: Decontamination description_md: > - This workflow identifies and removes contaminants from the assembly. + This workflow identifies and removes contaminants from the assembly. inputs: - datatypes: - fasta @@ -365,11 +365,11 @@ tabs:

- title_md: How can I assess the quality of my genome assembly? description_md: > - Once a genome has been assembled, it is important to assess the quality of the assembly, and in the first instance, this quality control (QC) can be achieved using the workflow described here. + Once a genome has been assembled, it is important to assess the quality of the assembly, and in the first instance, this quality control (QC) can be achieved using the workflow described here. button_md: Workflow tutorial button_link: https://australianbiocommons.github.io/how-to-guides/genome_assembly/assembly_qc - title_md: Galaxy Australia support description_md: > - Any user of Galaxy Australia can request support through an online form. + Any user of Galaxy Australia can request support through an online form. button_md: Request support button_link: /request/support From 12615a5cfd58b9eec09262aa2ee069fa9a97de61 Mon Sep 17 00:00:00 2001 From: Cameron Hyde Date: Fri, 13 Sep 2024 14:41:34 +1000 Subject: [PATCH 14/18] Fix embedded RSS feeds for safari --- subdomains/singlecell/templates/conclusion.html | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/subdomains/singlecell/templates/conclusion.html b/subdomains/singlecell/templates/conclusion.html index b751908f..486a59f8 100644 --- a/subdomains/singlecell/templates/conclusion.html +++ b/subdomains/singlecell/templates/conclusion.html @@ -18,10 +18,10 @@

News and Events

- +
- +
From 5be8e1d38927581e61cbf94b24ca2c154d3d2396 Mon Sep 17 00:00:00 2001 From: Cameron Hyde Date: Fri, 27 Sep 2024 13:40:22 +1000 Subject: [PATCH 15/18] Refactor labs with "communities" as root folder --- .../genome => communities/genome/lab}/CONTRIBUTORS | 0 .../genome/lab}/annotation.yml | 0 .../genome => communities/genome/lab}/assembly.yml | 0 .../genome => communities/genome/lab}/base.yml | 0 .../genome => communities/genome/lab}/data.yml | 0 .../genome/lab}/static/annotation-overview.png | Bin .../genome/lab}/static/assembly-overview.png | Bin .../genome/lab}/static/custom.css | 0 .../genome/lab}/static/logo.png | Bin .../genome/lab}/templates/conclusion.html | 0 .../genome/lab}/templates/footer.html | 0 .../genome/lab}/templates/intro.html | 2 +- .../genome/lab}/usegalaxy.org.au.yml | 0 .../lab}/usegalaxy.org.au/templates/conclusion.html | 0 .../lab}/usegalaxy.org.au/templates/footer.html | 0 .../lab}/usegalaxy.org.au/templates/intro.html | 0 .../proteomics/lab}/CONTRIBUTORS | 0 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rename {subdomains/genome => communities/genome/lab}/assembly.yml (100%) rename {subdomains/genome => communities/genome/lab}/base.yml (100%) rename {subdomains/genome => communities/genome/lab}/data.yml (100%) rename {subdomains/genome => communities/genome/lab}/static/annotation-overview.png (100%) rename {subdomains/genome => communities/genome/lab}/static/assembly-overview.png (100%) rename {subdomains/genome => communities/genome/lab}/static/custom.css (100%) rename {subdomains/genome => communities/genome/lab}/static/logo.png (100%) rename {subdomains/genome => communities/genome/lab}/templates/conclusion.html (100%) rename {subdomains/genome => communities/genome/lab}/templates/footer.html (100%) rename {subdomains/genome => communities/genome/lab}/templates/intro.html (94%) rename {subdomains/genome => communities/genome/lab}/usegalaxy.org.au.yml (100%) rename {subdomains/genome => communities/genome/lab}/usegalaxy.org.au/templates/conclusion.html (100%) rename {subdomains/genome => communities/genome/lab}/usegalaxy.org.au/templates/footer.html (100%) rename {subdomains/genome => communities/genome/lab}/usegalaxy.org.au/templates/intro.html (100%) rename {subdomains/proteomics => communities/proteomics/lab}/CONTRIBUTORS (100%) rename {subdomains/proteomics => communities/proteomics/lab}/base.yml (100%) rename {subdomains/proteomics => communities/proteomics/lab}/sections/conversion_modification.yml (100%) rename {subdomains/proteomics => communities/proteomics/lab}/sections/data.yml (100%) rename {subdomains/proteomics => communities/proteomics/lab}/sections/database_searching.yml (100%) rename {subdomains/proteomics => communities/proteomics/lab}/sections/dda_standardised_tools.yml (100%) rename {subdomains/proteomics => communities/proteomics/lab}/sections/dda_tmt.yml (100%) rename {subdomains/proteomics => communities/proteomics/lab}/sections/dia_standardised_tools.yml (100%) rename {subdomains/proteomics => communities/proteomics/lab}/static/custom.css (100%) rename {subdomains/proteomics => communities/proteomics/lab}/static/logo.png (100%) rename {subdomains/proteomics => communities/proteomics/lab}/templates/conclusion.html (100%) rename {subdomains/proteomics => communities/proteomics/lab}/templates/footer.html (100%) rename {subdomains/proteomics => communities/proteomics/lab}/templates/intro.html (97%) rename {subdomains/proteomics => communities/proteomics/lab}/usegalaxy.org.au.yml (100%) rename {subdomains/proteomics => communities/proteomics/lab}/usegalaxy.org.au/templates/conclusion.html (100%) rename {subdomains/proteomics => communities/proteomics/lab}/usegalaxy.org.au/templates/footer.html (100%) rename {subdomains => labs}/assets/site-switcher.png (100%) create mode 120000 labs/genome create mode 120000 labs/proteomics diff --git a/subdomains/genome/CONTRIBUTORS b/communities/genome/lab/CONTRIBUTORS similarity index 100% rename from subdomains/genome/CONTRIBUTORS rename to communities/genome/lab/CONTRIBUTORS diff --git a/subdomains/genome/annotation.yml b/communities/genome/lab/annotation.yml similarity index 100% rename from subdomains/genome/annotation.yml rename to communities/genome/lab/annotation.yml diff --git a/subdomains/genome/assembly.yml b/communities/genome/lab/assembly.yml similarity index 100% rename from subdomains/genome/assembly.yml rename to communities/genome/lab/assembly.yml diff --git a/subdomains/genome/base.yml b/communities/genome/lab/base.yml similarity index 100% rename from subdomains/genome/base.yml rename to communities/genome/lab/base.yml diff --git a/subdomains/genome/data.yml b/communities/genome/lab/data.yml similarity index 100% rename from subdomains/genome/data.yml rename to communities/genome/lab/data.yml diff --git a/subdomains/genome/static/annotation-overview.png b/communities/genome/lab/static/annotation-overview.png similarity index 100% rename from subdomains/genome/static/annotation-overview.png rename to communities/genome/lab/static/annotation-overview.png diff --git a/subdomains/genome/static/assembly-overview.png b/communities/genome/lab/static/assembly-overview.png similarity index 100% rename from subdomains/genome/static/assembly-overview.png rename to communities/genome/lab/static/assembly-overview.png diff --git a/subdomains/genome/static/custom.css b/communities/genome/lab/static/custom.css similarity index 100% rename from subdomains/genome/static/custom.css rename to communities/genome/lab/static/custom.css diff --git a/subdomains/genome/static/logo.png b/communities/genome/lab/static/logo.png similarity index 100% rename from subdomains/genome/static/logo.png rename to communities/genome/lab/static/logo.png diff --git a/subdomains/genome/templates/conclusion.html b/communities/genome/lab/templates/conclusion.html similarity index 100% rename from subdomains/genome/templates/conclusion.html rename to communities/genome/lab/templates/conclusion.html diff --git a/subdomains/genome/templates/footer.html b/communities/genome/lab/templates/footer.html similarity index 100% rename from subdomains/genome/templates/footer.html rename to communities/genome/lab/templates/footer.html diff --git a/subdomains/genome/templates/intro.html b/communities/genome/lab/templates/intro.html similarity index 94% rename from subdomains/genome/templates/intro.html rename to communities/genome/lab/templates/intro.html index 67c8467a..9460cb1b 100644 --- a/subdomains/genome/templates/intro.html +++ b/communities/genome/lab/templates/intro.html @@ -24,7 +24,7 @@
Galaxy {{ site_name }}
Switch between sites quickly using the dropdown in Galaxy {{ site_name }}'s main navigation bar (note, if your Galaxy server does not have this, please request that they install the "Site Switcher" plugin from Galaxy Australia): - Switch site button + Switch site button

Take me back to Galaxy {{ site_name }}

diff --git a/subdomains/genome/usegalaxy.org.au.yml b/communities/genome/lab/usegalaxy.org.au.yml similarity index 100% rename from subdomains/genome/usegalaxy.org.au.yml rename to communities/genome/lab/usegalaxy.org.au.yml diff --git a/subdomains/genome/usegalaxy.org.au/templates/conclusion.html b/communities/genome/lab/usegalaxy.org.au/templates/conclusion.html similarity index 100% rename from subdomains/genome/usegalaxy.org.au/templates/conclusion.html rename to communities/genome/lab/usegalaxy.org.au/templates/conclusion.html diff --git a/subdomains/genome/usegalaxy.org.au/templates/footer.html b/communities/genome/lab/usegalaxy.org.au/templates/footer.html similarity index 100% rename from subdomains/genome/usegalaxy.org.au/templates/footer.html rename to communities/genome/lab/usegalaxy.org.au/templates/footer.html diff --git a/subdomains/genome/usegalaxy.org.au/templates/intro.html b/communities/genome/lab/usegalaxy.org.au/templates/intro.html similarity index 100% rename from subdomains/genome/usegalaxy.org.au/templates/intro.html rename to communities/genome/lab/usegalaxy.org.au/templates/intro.html diff --git a/subdomains/proteomics/CONTRIBUTORS b/communities/proteomics/lab/CONTRIBUTORS similarity index 100% rename from subdomains/proteomics/CONTRIBUTORS rename to communities/proteomics/lab/CONTRIBUTORS diff --git a/subdomains/proteomics/base.yml b/communities/proteomics/lab/base.yml similarity index 100% rename from subdomains/proteomics/base.yml rename to communities/proteomics/lab/base.yml diff --git a/subdomains/proteomics/sections/conversion_modification.yml b/communities/proteomics/lab/sections/conversion_modification.yml similarity index 100% rename from subdomains/proteomics/sections/conversion_modification.yml rename to communities/proteomics/lab/sections/conversion_modification.yml diff --git a/subdomains/proteomics/sections/data.yml b/communities/proteomics/lab/sections/data.yml similarity index 100% rename from subdomains/proteomics/sections/data.yml rename to communities/proteomics/lab/sections/data.yml diff --git a/subdomains/proteomics/sections/database_searching.yml b/communities/proteomics/lab/sections/database_searching.yml similarity index 100% rename from subdomains/proteomics/sections/database_searching.yml rename to communities/proteomics/lab/sections/database_searching.yml diff --git a/subdomains/proteomics/sections/dda_standardised_tools.yml b/communities/proteomics/lab/sections/dda_standardised_tools.yml similarity index 100% rename from subdomains/proteomics/sections/dda_standardised_tools.yml rename to communities/proteomics/lab/sections/dda_standardised_tools.yml diff --git a/subdomains/proteomics/sections/dda_tmt.yml b/communities/proteomics/lab/sections/dda_tmt.yml similarity index 100% rename from subdomains/proteomics/sections/dda_tmt.yml rename to communities/proteomics/lab/sections/dda_tmt.yml diff --git a/subdomains/proteomics/sections/dia_standardised_tools.yml b/communities/proteomics/lab/sections/dia_standardised_tools.yml similarity index 100% rename from subdomains/proteomics/sections/dia_standardised_tools.yml rename to communities/proteomics/lab/sections/dia_standardised_tools.yml diff --git a/subdomains/proteomics/static/custom.css b/communities/proteomics/lab/static/custom.css similarity index 100% rename from subdomains/proteomics/static/custom.css rename to communities/proteomics/lab/static/custom.css diff --git a/subdomains/proteomics/static/logo.png b/communities/proteomics/lab/static/logo.png similarity index 100% rename from subdomains/proteomics/static/logo.png rename to communities/proteomics/lab/static/logo.png diff --git a/subdomains/proteomics/templates/conclusion.html b/communities/proteomics/lab/templates/conclusion.html similarity index 100% rename from subdomains/proteomics/templates/conclusion.html rename to communities/proteomics/lab/templates/conclusion.html diff --git a/subdomains/proteomics/templates/footer.html b/communities/proteomics/lab/templates/footer.html similarity index 100% rename from subdomains/proteomics/templates/footer.html rename to communities/proteomics/lab/templates/footer.html diff --git a/subdomains/proteomics/templates/intro.html b/communities/proteomics/lab/templates/intro.html similarity index 97% rename from subdomains/proteomics/templates/intro.html rename to communities/proteomics/lab/templates/intro.html index 89e30a5d..253193b1 100644 --- a/subdomains/proteomics/templates/intro.html +++ b/communities/proteomics/lab/templates/intro.html @@ -93,7 +93,7 @@
Galaxy {{ site_name }} {{ lab_name }}
and {{ lab_name }} ({{ subdomain }}.{{ root_domain }}) is easy - look for the icon in the top navigation bar to switch between sites: - Switch site button + Switch site button

{% if site_name == 'Australia' %} diff --git a/subdomains/proteomics/usegalaxy.org.au.yml b/communities/proteomics/lab/usegalaxy.org.au.yml similarity index 100% rename from subdomains/proteomics/usegalaxy.org.au.yml rename to communities/proteomics/lab/usegalaxy.org.au.yml diff --git a/subdomains/proteomics/usegalaxy.org.au/templates/conclusion.html b/communities/proteomics/lab/usegalaxy.org.au/templates/conclusion.html similarity index 100% rename from subdomains/proteomics/usegalaxy.org.au/templates/conclusion.html rename to communities/proteomics/lab/usegalaxy.org.au/templates/conclusion.html diff --git a/subdomains/proteomics/usegalaxy.org.au/templates/footer.html b/communities/proteomics/lab/usegalaxy.org.au/templates/footer.html similarity index 100% rename from subdomains/proteomics/usegalaxy.org.au/templates/footer.html rename to communities/proteomics/lab/usegalaxy.org.au/templates/footer.html diff --git a/subdomains/assets/site-switcher.png b/labs/assets/site-switcher.png similarity index 100% rename from subdomains/assets/site-switcher.png rename to labs/assets/site-switcher.png diff --git a/labs/genome b/labs/genome new file mode 120000 index 00000000..257b6474 --- /dev/null +++ b/labs/genome @@ -0,0 +1 @@ +../communities/genome/lab \ No newline at end of file diff --git a/labs/proteomics b/labs/proteomics new file mode 120000 index 00000000..c05c4e68 --- /dev/null +++ b/labs/proteomics @@ -0,0 +1 @@ +../communities/proteomics/lab \ No newline at end of file From 4724cf208863a47f741a7a2b4fa457f2dbbb1489 Mon Sep 17 00:00:00 2001 From: Cameron Hyde Date: Sun, 29 Sep 2024 13:18:52 +1000 Subject: [PATCH 16/18] Rename 'subdomain' -> 'lab' and add spoc lab link --- communities/spoc/{subdomain => lab}/CONTRIBUTORS | 0 communities/spoc/{subdomain => lab}/LICENSE | 0 communities/spoc/{subdomain => lab}/README.md | 0 communities/spoc/{subdomain => lab}/base.yml | 0 communities/spoc/{subdomain => lab}/sections/1_beginner.yml | 0 communities/spoc/{subdomain => lab}/sections/2_intermediate.yml | 0 communities/spoc/{subdomain => lab}/sections/3_advanced.yml | 0 communities/spoc/{subdomain => lab}/sections/4_community.yml | 0 communities/spoc/{subdomain => lab}/static/custom.css | 0 communities/spoc/{subdomain => lab}/static/logo_single_cell.svg | 0 communities/spoc/{subdomain => lab}/templates/conclusion.html | 0 communities/spoc/{subdomain => lab}/templates/footer.html | 0 communities/spoc/{subdomain => lab}/templates/intro.html | 0 communities/spoc/{subdomain => lab}/tool_panel/hca-scanpy.yml | 0 .../spoc/{subdomain => lab}/tool_panel/hca-scanpy.yml.lock | 0 communities/spoc/{subdomain => lab}/tool_panel/hicexplorer.yml | 0 .../spoc/{subdomain => lab}/tool_panel/hicexplorer.yml.lock | 0 communities/spoc/{subdomain => lab}/tool_panel/import.yml | 0 communities/spoc/{subdomain => lab}/tool_panel/import.yml.lock | 0 communities/spoc/{subdomain => lab}/tool_panel/multiomics.yml | 0 .../spoc/{subdomain => lab}/tool_panel/multiomics.yml.lock | 0 communities/spoc/{subdomain => lab}/tool_panel/sccaf.yml | 0 communities/spoc/{subdomain => lab}/tool_panel/sccaf.yml.lock | 0 communities/spoc/{subdomain => lab}/tool_panel/seurat.yml | 0 communities/spoc/{subdomain => lab}/tool_panel/seurat.yml.lock | 0 communities/spoc/{subdomain => lab}/tool_panel/single_cell.yml | 0 .../spoc/{subdomain => lab}/tool_panel/single_cell.yml.lock | 0 communities/spoc/{subdomain => lab}/tool_panel/spatial.yml | 0 communities/spoc/{subdomain => lab}/tool_panel/spatial.yml.lock | 0 .../spoc/{subdomain => lab}/tool_panel/tool_panel_view.yml | 0 .../spoc/{subdomain => lab}/tool_panel/usegalaxy.org.au.yml | 0 communities/spoc/{subdomain => lab}/usegalaxy.eu.yml | 0 .../spoc/{subdomain => lab}/usegalaxy.eu/static/custom.css | 0 .../spoc/{subdomain => lab}/usegalaxy.eu/templates/footer.html | 0 communities/spoc/{subdomain => lab}/usegalaxy.org.au.yml | 0 .../{subdomain => lab}/usegalaxy.org.au/templates/footer.html | 0 communities/spoc/{subdomain => lab}/usegalaxy.org.yml | 0 labs/spoc | 1 + 38 files changed, 1 insertion(+) rename communities/spoc/{subdomain => lab}/CONTRIBUTORS (100%) rename communities/spoc/{subdomain => lab}/LICENSE (100%) rename communities/spoc/{subdomain => lab}/README.md (100%) rename communities/spoc/{subdomain => lab}/base.yml (100%) rename communities/spoc/{subdomain => lab}/sections/1_beginner.yml (100%) rename communities/spoc/{subdomain => lab}/sections/2_intermediate.yml (100%) rename communities/spoc/{subdomain => lab}/sections/3_advanced.yml (100%) rename communities/spoc/{subdomain => lab}/sections/4_community.yml (100%) rename communities/spoc/{subdomain => lab}/static/custom.css (100%) rename communities/spoc/{subdomain => lab}/static/logo_single_cell.svg (100%) rename communities/spoc/{subdomain => lab}/templates/conclusion.html (100%) rename communities/spoc/{subdomain => lab}/templates/footer.html (100%) rename communities/spoc/{subdomain => lab}/templates/intro.html (100%) rename communities/spoc/{subdomain => lab}/tool_panel/hca-scanpy.yml (100%) rename communities/spoc/{subdomain => lab}/tool_panel/hca-scanpy.yml.lock (100%) rename communities/spoc/{subdomain => lab}/tool_panel/hicexplorer.yml (100%) rename communities/spoc/{subdomain => lab}/tool_panel/hicexplorer.yml.lock (100%) rename communities/spoc/{subdomain => lab}/tool_panel/import.yml (100%) rename communities/spoc/{subdomain => lab}/tool_panel/import.yml.lock (100%) rename communities/spoc/{subdomain => lab}/tool_panel/multiomics.yml (100%) rename communities/spoc/{subdomain => lab}/tool_panel/multiomics.yml.lock (100%) rename communities/spoc/{subdomain => lab}/tool_panel/sccaf.yml (100%) rename communities/spoc/{subdomain => lab}/tool_panel/sccaf.yml.lock (100%) rename communities/spoc/{subdomain => lab}/tool_panel/seurat.yml (100%) rename communities/spoc/{subdomain => lab}/tool_panel/seurat.yml.lock (100%) rename communities/spoc/{subdomain => lab}/tool_panel/single_cell.yml (100%) rename communities/spoc/{subdomain => lab}/tool_panel/single_cell.yml.lock (100%) rename communities/spoc/{subdomain => lab}/tool_panel/spatial.yml (100%) rename communities/spoc/{subdomain => lab}/tool_panel/spatial.yml.lock (100%) rename communities/spoc/{subdomain => lab}/tool_panel/tool_panel_view.yml (100%) rename communities/spoc/{subdomain => lab}/tool_panel/usegalaxy.org.au.yml (100%) rename communities/spoc/{subdomain => lab}/usegalaxy.eu.yml (100%) rename communities/spoc/{subdomain => lab}/usegalaxy.eu/static/custom.css (100%) rename communities/spoc/{subdomain => lab}/usegalaxy.eu/templates/footer.html (100%) rename communities/spoc/{subdomain => lab}/usegalaxy.org.au.yml (100%) rename communities/spoc/{subdomain => lab}/usegalaxy.org.au/templates/footer.html (100%) rename communities/spoc/{subdomain => lab}/usegalaxy.org.yml (100%) create mode 120000 labs/spoc diff --git a/communities/spoc/subdomain/CONTRIBUTORS b/communities/spoc/lab/CONTRIBUTORS similarity index 100% rename from communities/spoc/subdomain/CONTRIBUTORS rename to communities/spoc/lab/CONTRIBUTORS diff --git a/communities/spoc/subdomain/LICENSE b/communities/spoc/lab/LICENSE similarity index 100% rename from communities/spoc/subdomain/LICENSE rename to communities/spoc/lab/LICENSE diff --git a/communities/spoc/subdomain/README.md b/communities/spoc/lab/README.md similarity index 100% rename from communities/spoc/subdomain/README.md rename to communities/spoc/lab/README.md diff --git a/communities/spoc/subdomain/base.yml b/communities/spoc/lab/base.yml similarity index 100% rename from communities/spoc/subdomain/base.yml rename to communities/spoc/lab/base.yml diff --git a/communities/spoc/subdomain/sections/1_beginner.yml b/communities/spoc/lab/sections/1_beginner.yml similarity index 100% rename from communities/spoc/subdomain/sections/1_beginner.yml rename to communities/spoc/lab/sections/1_beginner.yml diff --git a/communities/spoc/subdomain/sections/2_intermediate.yml b/communities/spoc/lab/sections/2_intermediate.yml similarity index 100% rename from communities/spoc/subdomain/sections/2_intermediate.yml rename to communities/spoc/lab/sections/2_intermediate.yml diff --git a/communities/spoc/subdomain/sections/3_advanced.yml b/communities/spoc/lab/sections/3_advanced.yml similarity index 100% rename from communities/spoc/subdomain/sections/3_advanced.yml rename to communities/spoc/lab/sections/3_advanced.yml diff --git a/communities/spoc/subdomain/sections/4_community.yml b/communities/spoc/lab/sections/4_community.yml similarity index 100% rename from communities/spoc/subdomain/sections/4_community.yml rename to communities/spoc/lab/sections/4_community.yml diff --git a/communities/spoc/subdomain/static/custom.css b/communities/spoc/lab/static/custom.css similarity index 100% rename from communities/spoc/subdomain/static/custom.css rename to communities/spoc/lab/static/custom.css diff --git a/communities/spoc/subdomain/static/logo_single_cell.svg b/communities/spoc/lab/static/logo_single_cell.svg similarity index 100% rename from communities/spoc/subdomain/static/logo_single_cell.svg rename to communities/spoc/lab/static/logo_single_cell.svg diff --git a/communities/spoc/subdomain/templates/conclusion.html b/communities/spoc/lab/templates/conclusion.html similarity index 100% rename from communities/spoc/subdomain/templates/conclusion.html rename to communities/spoc/lab/templates/conclusion.html diff --git a/communities/spoc/subdomain/templates/footer.html b/communities/spoc/lab/templates/footer.html similarity index 100% rename from communities/spoc/subdomain/templates/footer.html rename to communities/spoc/lab/templates/footer.html diff --git a/communities/spoc/subdomain/templates/intro.html b/communities/spoc/lab/templates/intro.html similarity index 100% rename from communities/spoc/subdomain/templates/intro.html rename to communities/spoc/lab/templates/intro.html diff --git a/communities/spoc/subdomain/tool_panel/hca-scanpy.yml b/communities/spoc/lab/tool_panel/hca-scanpy.yml similarity index 100% rename from communities/spoc/subdomain/tool_panel/hca-scanpy.yml rename to communities/spoc/lab/tool_panel/hca-scanpy.yml diff --git a/communities/spoc/subdomain/tool_panel/hca-scanpy.yml.lock b/communities/spoc/lab/tool_panel/hca-scanpy.yml.lock similarity index 100% rename from communities/spoc/subdomain/tool_panel/hca-scanpy.yml.lock rename to communities/spoc/lab/tool_panel/hca-scanpy.yml.lock diff --git a/communities/spoc/subdomain/tool_panel/hicexplorer.yml b/communities/spoc/lab/tool_panel/hicexplorer.yml similarity index 100% rename from communities/spoc/subdomain/tool_panel/hicexplorer.yml rename to communities/spoc/lab/tool_panel/hicexplorer.yml diff --git a/communities/spoc/subdomain/tool_panel/hicexplorer.yml.lock b/communities/spoc/lab/tool_panel/hicexplorer.yml.lock similarity index 100% rename from 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a/communities/spoc/subdomain/tool_panel/seurat.yml.lock b/communities/spoc/lab/tool_panel/seurat.yml.lock similarity index 100% rename from communities/spoc/subdomain/tool_panel/seurat.yml.lock rename to communities/spoc/lab/tool_panel/seurat.yml.lock diff --git a/communities/spoc/subdomain/tool_panel/single_cell.yml b/communities/spoc/lab/tool_panel/single_cell.yml similarity index 100% rename from communities/spoc/subdomain/tool_panel/single_cell.yml rename to communities/spoc/lab/tool_panel/single_cell.yml diff --git a/communities/spoc/subdomain/tool_panel/single_cell.yml.lock b/communities/spoc/lab/tool_panel/single_cell.yml.lock similarity index 100% rename from communities/spoc/subdomain/tool_panel/single_cell.yml.lock rename to communities/spoc/lab/tool_panel/single_cell.yml.lock diff --git a/communities/spoc/subdomain/tool_panel/spatial.yml b/communities/spoc/lab/tool_panel/spatial.yml similarity index 100% rename from communities/spoc/subdomain/tool_panel/spatial.yml rename to communities/spoc/lab/tool_panel/spatial.yml diff --git a/communities/spoc/subdomain/tool_panel/spatial.yml.lock b/communities/spoc/lab/tool_panel/spatial.yml.lock similarity index 100% rename from communities/spoc/subdomain/tool_panel/spatial.yml.lock rename to communities/spoc/lab/tool_panel/spatial.yml.lock diff --git a/communities/spoc/subdomain/tool_panel/tool_panel_view.yml b/communities/spoc/lab/tool_panel/tool_panel_view.yml similarity index 100% rename from communities/spoc/subdomain/tool_panel/tool_panel_view.yml rename to communities/spoc/lab/tool_panel/tool_panel_view.yml diff --git a/communities/spoc/subdomain/tool_panel/usegalaxy.org.au.yml b/communities/spoc/lab/tool_panel/usegalaxy.org.au.yml similarity index 100% rename from communities/spoc/subdomain/tool_panel/usegalaxy.org.au.yml rename to communities/spoc/lab/tool_panel/usegalaxy.org.au.yml diff --git a/communities/spoc/subdomain/usegalaxy.eu.yml b/communities/spoc/lab/usegalaxy.eu.yml similarity index 100% rename from communities/spoc/subdomain/usegalaxy.eu.yml rename to communities/spoc/lab/usegalaxy.eu.yml diff --git a/communities/spoc/subdomain/usegalaxy.eu/static/custom.css b/communities/spoc/lab/usegalaxy.eu/static/custom.css similarity index 100% rename from communities/spoc/subdomain/usegalaxy.eu/static/custom.css rename to communities/spoc/lab/usegalaxy.eu/static/custom.css diff --git a/communities/spoc/subdomain/usegalaxy.eu/templates/footer.html b/communities/spoc/lab/usegalaxy.eu/templates/footer.html similarity index 100% rename from communities/spoc/subdomain/usegalaxy.eu/templates/footer.html rename to communities/spoc/lab/usegalaxy.eu/templates/footer.html diff --git a/communities/spoc/subdomain/usegalaxy.org.au.yml b/communities/spoc/lab/usegalaxy.org.au.yml similarity index 100% rename from communities/spoc/subdomain/usegalaxy.org.au.yml rename to communities/spoc/lab/usegalaxy.org.au.yml diff --git a/communities/spoc/subdomain/usegalaxy.org.au/templates/footer.html b/communities/spoc/lab/usegalaxy.org.au/templates/footer.html similarity index 100% rename from communities/spoc/subdomain/usegalaxy.org.au/templates/footer.html rename to communities/spoc/lab/usegalaxy.org.au/templates/footer.html diff --git a/communities/spoc/subdomain/usegalaxy.org.yml b/communities/spoc/lab/usegalaxy.org.yml similarity index 100% rename from communities/spoc/subdomain/usegalaxy.org.yml rename to communities/spoc/lab/usegalaxy.org.yml diff --git a/labs/spoc b/labs/spoc new file mode 120000 index 00000000..ada10927 --- /dev/null +++ b/labs/spoc @@ -0,0 +1 @@ +../communities/spoc/lab/ \ No newline at end of file From 37a1235598b12e4d510a2e6ee27c6843e6873f47 Mon Sep 17 00:00:00 2001 From: Cameron Hyde Date: Sun, 29 Sep 2024 13:42:58 +1000 Subject: [PATCH 17/18] Update single cell lab README --- communities/spoc/lab/README.md | 15 +++++++++++++-- 1 file changed, 13 insertions(+), 2 deletions(-) diff --git a/communities/spoc/lab/README.md b/communities/spoc/lab/README.md index 0c71ba4d..a067b8eb 100644 --- a/communities/spoc/lab/README.md +++ b/communities/spoc/lab/README.md @@ -1,2 +1,13 @@ -# Lab_testing -Testing of configurations of Galaxy Lab yamls +# Single Cell Lab + +This Galaxy Lab was developed by Wendi Bacon (@nomadscientist) and Cameron Hyde +(@neoformit). + +Render this Galaxy Lab webpage: + +https://labs.usegalaxy.org.au/?content_root=https://raw.githubusercontent.com/galaxyproject/galaxy_codex/refs/heads/main/communities/spoc/lab/base.yml + + +Render the EU version of Galaxy Lab webpage: + +https://labs.usegalaxy.org.au/?content_root=https://raw.githubusercontent.com/galaxyproject/galaxy_codex/refs/heads/main/communities/spoc/lab/usegalaxy.eu.yml From 5785d99371d427e6ad2c3477658b9faf2099ad15 Mon Sep 17 00:00:00 2001 From: Cameron Hyde Date: Tue, 1 Oct 2024 10:57:26 +1000 Subject: [PATCH 18/18] Move global labs files to communities/all/labs/ --- .../all/labs}/assets/site-switcher.png | Bin communities/all/labs/genome | 1 + communities/all/labs/proteomics | 1 + communities/all/labs/single-cell | 1 + communities/all/labs/spoc | 1 + labs/genome | 1 - labs/proteomics | 1 - labs/spoc | 1 - 8 files changed, 4 insertions(+), 3 deletions(-) rename {labs => communities/all/labs}/assets/site-switcher.png (100%) create mode 120000 communities/all/labs/genome create mode 120000 communities/all/labs/proteomics create mode 120000 communities/all/labs/single-cell create mode 120000 communities/all/labs/spoc delete mode 120000 labs/genome delete mode 120000 labs/proteomics delete mode 120000 labs/spoc diff --git a/labs/assets/site-switcher.png b/communities/all/labs/assets/site-switcher.png similarity index 100% rename from labs/assets/site-switcher.png rename to communities/all/labs/assets/site-switcher.png diff --git a/communities/all/labs/genome b/communities/all/labs/genome new file mode 120000 index 00000000..27bc63d1 --- /dev/null +++ b/communities/all/labs/genome @@ -0,0 +1 @@ +../../genome/lab/ \ No newline at end of file diff --git a/communities/all/labs/proteomics b/communities/all/labs/proteomics new file mode 120000 index 00000000..fd3243f1 --- /dev/null +++ b/communities/all/labs/proteomics @@ -0,0 +1 @@ +../../proteomics/lab/ \ No newline at end of file diff --git a/communities/all/labs/single-cell b/communities/all/labs/single-cell new file mode 120000 index 00000000..67ecc16e --- /dev/null +++ b/communities/all/labs/single-cell @@ -0,0 +1 @@ +../../spoc/lab/ \ No newline at end of file diff --git a/communities/all/labs/spoc b/communities/all/labs/spoc new file mode 120000 index 00000000..67ecc16e --- /dev/null +++ b/communities/all/labs/spoc @@ -0,0 +1 @@ +../../spoc/lab/ \ No newline at end of file diff --git a/labs/genome b/labs/genome deleted file mode 120000 index 257b6474..00000000 --- a/labs/genome +++ /dev/null @@ -1 +0,0 @@ -../communities/genome/lab \ No newline at end of file diff --git a/labs/proteomics b/labs/proteomics deleted file mode 120000 index c05c4e68..00000000 --- a/labs/proteomics +++ /dev/null @@ -1 +0,0 @@ -../communities/proteomics/lab \ No newline at end of file diff --git a/labs/spoc b/labs/spoc deleted file mode 120000 index ada10927..00000000 --- a/labs/spoc +++ /dev/null @@ -1 +0,0 @@ -../communities/spoc/lab/ \ No newline at end of file

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