From 4d079c84616d935d9991ca6ebe5e07aea093f43e Mon Sep 17 00:00:00 2001
From: Martin Cech <cech.marten@gmail.com>
Date: Tue, 24 Jan 2017 11:23:41 -0500
Subject: [PATCH 01/40] migrate in kraken, vflib, samtools

---
 tool_collections/kraken/README.rst            |   92 ++
 tool_collections/kraken/database.idx          |  Bin 0 -> 8208 bytes
 tool_collections/kraken/database.kdb          |  Bin 0 -> 1055671 bytes
 tool_collections/kraken/kraken/.shed.yml      |   16 +
 tool_collections/kraken/kraken/README.rst     |    1 +
 tool_collections/kraken/kraken/kraken.xml     |  164 +++
 tool_collections/kraken/kraken/macros.xml     |    1 +
 .../kraken/kraken/test-data/kraken_test1.fa   |   70 +
 .../kraken/test-data/kraken_test1_output.tab  |    4 +
 .../kraken/kraken/test-data/test_database.loc |    1 +
 .../kraken/test-data/test_db/database.idx     |    1 +
 .../kraken/test-data/test_db/database.kdb     |    1 +
 .../test-data/test_db/taxonomy/names.dmp      |    1 +
 .../test-data/test_db/taxonomy/nodes.dmp      |    1 +
 .../tool-data/kraken_databases.loc.sample     |    1 +
 .../kraken/tool_data_table_conf.xml.sample    |    1 +
 .../kraken/tool_data_table_conf.xml.test      |    1 +
 .../kraken/kraken/tool_dependencies.xml       |    6 +
 .../kraken/kraken_databases.loc.sample        |    0
 .../kraken/kraken_filter/.shed.yml            |   16 +
 .../kraken/kraken_filter/README.rst           |    1 +
 .../kraken/kraken_filter/kraken-filter.xml    |   63 +
 .../kraken/kraken_filter/macros.xml           |    1 +
 .../test-data/kraken_filter_test1.tab         |    4 +
 .../test-data/kraken_filter_test1_output.tab  |    4 +
 .../kraken_filter/test-data/kraken_test1.fa   |   70 +
 .../kraken_filter/test-data/test_database.loc |    1 +
 .../test-data/test_db/database.idx            |    1 +
 .../test-data/test_db/database.kdb            |    1 +
 .../test-data/test_db/taxonomy/names.dmp      |    1 +
 .../test-data/test_db/taxonomy/nodes.dmp      |    1 +
 .../tool-data/kraken_databases.loc.sample     |    1 +
 .../tool_data_table_conf.xml.sample           |    1 +
 .../tool_data_table_conf.xml.test             |    1 +
 .../kraken_filter/tool_dependencies.xml       |    6 +
 .../kraken/kraken_report/.shed.yml            |   16 +
 .../kraken/kraken_report/README.rst           |    1 +
 .../kraken_report/kraken-mpa-report.xml       |   83 ++
 .../kraken/kraken_report/kraken-report.xml    |   58 +
 .../kraken/kraken_report/macros.xml           |    1 +
 .../test-data/kraken_mpa_report_input1.tab    |    4 +
 .../test-data/kraken_mpa_report_input2.tab    |    4 +
 .../kraken_mpa_report_test1_output.tab        |    8 +
 .../test-data/kraken_report_test1.tab         |    4 +
 .../test-data/kraken_report_test1_output.tab  |   11 +
 .../kraken_report/test-data/test_database.loc |    1 +
 .../test-data/test_db/database.idx            |    1 +
 .../test-data/test_db/database.kdb            |    1 +
 .../test-data/test_db/taxonomy/names.dmp      |    1 +
 .../test-data/test_db/taxonomy/nodes.dmp      |    1 +
 .../tool-data/kraken_databases.loc.sample     |    1 +
 .../tool_data_table_conf.xml.sample           |    1 +
 .../tool_data_table_conf.xml.test             |    1 +
 .../kraken_report/tool_dependencies.xml       |    6 +
 .../kraken/kraken_translate/.shed.yml         |   16 +
 .../kraken/kraken_translate/README.rst        |    1 +
 .../kraken_translate/kraken-translate.xml     |   60 +
 .../kraken/kraken_translate/macros.xml        |    1 +
 .../test-data/kraken_translate_test1.tab      |    4 +
 .../kraken_translate_test1_output.tab         |    4 +
 .../test-data/test_database.loc               |    1 +
 .../test-data/test_db/database.idx            |    1 +
 .../test-data/test_db/database.kdb            |    1 +
 .../test-data/test_db/taxonomy/names.dmp      |    1 +
 .../test-data/test_db/taxonomy/nodes.dmp      |    1 +
 .../tool-data/kraken_databases.loc.sample     |    1 +
 .../tool_data_table_conf.xml.sample           |    1 +
 .../tool_data_table_conf.xml.test             |    1 +
 .../kraken_translate/tool_dependencies.xml    |    6 +
 tool_collections/kraken/macros.xml            |   31 +
 tool_collections/kraken/names.dmp             |   74 ++
 tool_collections/kraken/nodes.dmp             |   10 +
 tool_collections/kraken/test_database.loc     |    1 +
 .../kraken/tool_data_table_conf.xml.sample    |    8 +
 .../kraken/tool_data_table_conf.xml.test      |    8 +
 .../samtools/bam_to_cram/.shed.yml            |   13 +
 .../samtools/bam_to_cram/macros.xml           |    1 +
 .../bam_to_cram/samtools_bam_to_cram.xml      |  125 ++
 .../samtools/bam_to_cram/test-data/test.bam   |  Bin 0 -> 857 bytes
 .../samtools/bam_to_cram/test-data/test.bed   |    1 +
 .../samtools/bam_to_cram/test-data/test.cram  |  Bin 0 -> 11571 bytes
 .../samtools/bam_to_cram/test-data/test.fa    |    3 +
 .../samtools/bam_to_cram/test-data/test.sam   |   15 +
 .../bam_to_cram/tool_dependencies.xml         |    1 +
 .../samtools/bam_to_sam/.shed.yml             |   11 +
 .../samtools/bam_to_sam/bam_to_sam.xml        |   54 +
 .../samtools/bam_to_sam/macros.xml            |    1 +
 .../bam_to_sam/test-data/bam_to_sam_in1.bam   |  Bin 0 -> 671 bytes
 .../bam_to_sam/test-data/bam_to_sam_in1.sam   |   14 +
 .../bam_to_sam/test-data/bam_to_sam_in2.bam   |  Bin 0 -> 351 bytes
 .../bam_to_sam/test-data/bam_to_sam_in2.sam   |   25 +
 .../bam_to_sam/test-data/bam_to_sam_out1.sam  |   14 +
 .../bam_to_sam/test-data/bam_to_sam_out2.sam  |    6 +
 .../bam_to_sam/test-data/bam_to_sam_out3.sam  |    8 +
 .../samtools/bam_to_sam/tool_dependencies.xml |    1 +
 .../samtools/cram_to_bam/.shed.yml            |   11 +
 .../samtools/cram_to_bam/macros.xml           |    1 +
 .../cram_to_bam/samtools_cram_to_bam.xml      |  116 ++
 .../samtools/cram_to_bam/test-data            |    1 +
 .../cram_to_bam/tool_dependencies.xml         |    1 +
 tool_collections/samtools/macros.xml          |   71 +
 .../samtools/sam_to_bam/.shed.yml             |   11 +
 .../samtools/sam_to_bam/macros.xml            |    1 +
 .../samtools/sam_to_bam/sam_to_bam.xml        |   89 ++
 .../test-data/cached_locally/all_fasta.loc    |    1 +
 .../test-data/cached_locally/chr_m.fasta      |    1 +
 .../test-data/cached_locally/chr_m.fasta.fai  |    1 +
 .../cached_locally/fasta_indexes.loc          |    1 +
 .../samtools/sam_to_bam/test-data/chr_m.fasta |  335 +++++
 .../sam_to_bam/test-data/chr_m.fasta.fai      |    1 +
 .../sam_to_bam/test-data/sam_to_bam_in1.sam   |   13 +
 .../test-data/sam_to_bam_noheader_in2.sam     |   10 +
 .../sam_to_bam/test-data/sam_to_bam_out1.bam  |  Bin 0 -> 610 bytes
 .../sam_to_bam/test-data/sam_to_bam_out2.bam  |  Bin 0 -> 610 bytes
 .../sam_to_bam/test-data/sam_to_bam_out3.bam  |  Bin 0 -> 593 bytes
 .../tool-data/fasta_indexes.loc.sample        |   29 +
 .../tool_data_table_conf.xml.sample           |    7 +
 .../sam_to_bam/tool_data_table_conf.xml.test  |   10 +
 .../samtools/sam_to_bam/tool_dependencies.xml |    1 +
 .../samtools/samtools_bedcov/.shed.yml        |   11 +
 .../samtools/samtools_bedcov/macros.xml       |    1 +
 .../samtools_bedcov/samtools_bedcov.xml       |   57 +
 .../samtools_bedcov/test-data/eboVir3.1.bed   |    3 +
 .../samtools_bedcov/test-data/eboVir3.2.bam   |  Bin 0 -> 120217 bytes
 .../samtools_bedcov/test-data/eboVir3.2.bed   |    3 +
 .../samtools_bedcov/test-data/eboVir3.bam     |  Bin 0 -> 82464 bytes
 .../test-data/samtools_bedcov_out1.tab        |    3 +
 .../test-data/samtools_bedcov_out2.tab        |    3 +
 .../samtools_bedcov/tool_dependencies.xml     |    1 +
 .../samtools/samtools_calmd/.shed.yml         |   10 +
 .../samtools/samtools_calmd/macros.xml        |    1 +
 .../samtools_calmd/samtools_calmd.xml         |   99 ++
 .../samtools_calmd/test-data/phiX.bam         |  Bin 0 -> 350391 bytes
 .../samtools_calmd/test-data/phiX.fasta       |   79 ++
 .../test-data/samtools_calmd_out_1.bam        |  Bin 0 -> 350305 bytes
 .../test-data/samtools_calmd_out_2.bam        |  Bin 0 -> 323543 bytes
 .../tool-data/fasta_indexes.loc.sample        |   29 +
 .../tool_data_table_conf.xml.sample           |    7 +
 .../samtools_calmd/tool_dependencies.xml      |    1 +
 .../samtools/samtools_flagstat/.shed.yml      |   11 +
 .../samtools/samtools_flagstat/macros.xml     |    1 +
 .../samtools_flagstat/samtools_flagstat.xml   |   45 +
 .../test-data/samtools_flagstat_input1.bam    |  Bin 0 -> 17406 bytes
 .../test-data/samtools_flagstat_out1.txt      |   13 +
 .../samtools_flagstat/tool_dependencies.xml   |    1 +
 .../samtools/samtools_idxstats/.shed.yml      |   12 +
 .../samtools/samtools_idxstats/macros.xml     |    1 +
 .../samtools_idxstats/samtools_idxstats.xml   |   72 ++
 .../samtools_idxstats/test-data/phiX.bam      |  Bin 0 -> 38376 bytes
 .../test-data/samtools_idxstats_out.tabular   |    2 +
 .../samtools_idxstats/tool_dependencies.xml   |    1 +
 .../samtools/samtools_mpileup/.shed.yml       |   13 +
 .../samtools/samtools_mpileup/macros.xml      |    1 +
 .../samtools_mpileup/samtools_mpileup.xml     |  372 ++++++
 .../samtools_mpileup/test-data/phiX.bam       |  Bin 0 -> 350391 bytes
 .../samtools_mpileup/test-data/phiX.fasta     |   79 ++
 .../samtools_mpileup/test-data/phiX_1.bam     |  Bin 0 -> 5319 bytes
 .../mpileup/samtools_mpileup_out_1.pileup     |   43 +
 .../mpileup/samtools_mpileup_out_2.bcf        |  Bin 0 -> 426 bytes
 .../test-data/samtools_mpileup_in_1.bam       |  Bin 0 -> 1417 bytes
 .../test-data/samtools_mpileup_out_1.pileup   |   43 +
 .../test-data/samtools_mpileup_out_2.vcf      |   22 +
 .../test-data/samtools_mpileup_out_4.vcf      |   22 +
 .../tool-data/fasta_indexes.loc.sample        |   29 +
 .../tool_data_table_conf.xml.sample           |    7 +
 .../samtools_mpileup/tool_dependencies.xml    |    1 +
 .../samtools/samtools_phase/.shed.yml         |    9 +
 .../samtools/samtools_phase/macros.xml        |    1 +
 .../samtools_phase/samtools_phase.xml         |  130 ++
 .../samtools_phase/test-data/empty_file.bam   |    0
 .../test-data/samtools_phase_in_1.bam         |  Bin 0 -> 2538 bytes
 .../test-data/samtools_phase_in_2.bam         |  Bin 0 -> 6498 bytes
 .../test-data/samtools_phase_out_1_log.txt    |   30 +
 .../test-data/samtools_phase_out_1_phase0.bam |  Bin 0 -> 1801 bytes
 .../test-data/samtools_phase_out_1_phase1.bam |  Bin 0 -> 1280 bytes
 .../test-data/samtools_phase_out_2_log.txt    |   65 +
 .../test-data/samtools_phase_out_2_phase0.bam |  Bin 0 -> 2875 bytes
 .../test-data/samtools_phase_out_2_phase1.bam |  Bin 0 -> 4250 bytes
 .../test-data/samtools_phase_out_3_log.txt    |   65 +
 .../test-data/samtools_phase_out_3_phase0.bam |  Bin 0 -> 2875 bytes
 .../test-data/samtools_phase_out_3_phase1.bam |  Bin 0 -> 4344 bytes
 .../samtools_phase/tool_dependencies.xml      |    1 +
 .../samtools/samtools_reheader/.shed.yml      |   10 +
 .../samtools/samtools_reheader/macros.xml     |    1 +
 .../samtools_reheader/samtools_reheader.xml   |   32 +
 .../test-data/reheader_input_file.bam         |  Bin 0 -> 17425 bytes
 .../test-data/reheader_input_header.bam       |  Bin 0 -> 802 bytes
 .../test-data/reheader_out.bam                |  Bin 0 -> 17452 bytes
 .../samtools_reheader/tool_dependencies.xml   |    1 +
 .../samtools/samtools_rmdup/.shed.yml         |   14 +
 .../samtools/samtools_rmdup/macros.xml        |    1 +
 .../samtools_rmdup/samtools_rmdup.xml         |   56 +
 .../test-data/samtools-rmdup-input1.bam       |  Bin 0 -> 17406 bytes
 .../test-data/samtools-rmdup-test1.bam        |  Bin 0 -> 17406 bytes
 .../samtools_rmdup/tool_dependencies.xml      |    6 +
 .../samtools/samtools_slice_bam/.shed.yml     |   11 +
 .../samtools/samtools_slice_bam/macros.xml    |    1 +
 .../samtools_slice_bam/samtools_slice_bam.xml |  123 ++
 .../test-data/bam-slice-input.bam             |  Bin 0 -> 17406 bytes
 .../test-data/bam-slice-test1.bam             |  Bin 0 -> 4454 bytes
 .../test-data/bam-slice-test2.bam             |  Bin 0 -> 6936 bytes
 .../test-data/bam-slice-test3.bam             |  Bin 0 -> 6936 bytes
 .../test-data/bam-slice.bed                   |   38 +
 .../samtools_slice_bam/tool_dependencies.xml  |    1 +
 .../samtools/samtools_sort/.shed.yml          |   11 +
 .../samtools/samtools_sort/macros.xml         |    1 +
 .../samtools/samtools_sort/samtools_sort.xml  |   40 +
 .../samtools/samtools_sort/test-data/1.bam    |  Bin 0 -> 3592 bytes
 .../samtools_sort/test-data/1_sort.bam        |  Bin 0 -> 3537 bytes
 .../test-data/1_sort_read_names.bam           |  Bin 0 -> 3539 bytes
 .../samtools_sort/tool_dependencies.xml       |    1 +
 .../samtools/samtools_split/.shed.yml         |   11 +
 .../samtools/samtools_split/macros.xml        |    1 +
 .../samtools_split/samtools_split.xml         |   47 +
 .../samtools_split/test-data/phiX.bam         |  Bin 0 -> 58479 bytes
 .../test-data/phiX_Elephant.bam               |  Bin 0 -> 38913 bytes
 .../samtools_split/test-data/phiX_Human.bam   |  Bin 0 -> 38922 bytes
 .../samtools_split/test-data/phiX_Mouse.bam   |  Bin 0 -> 10763 bytes
 .../test-data/samtools_split_out.bam          |  Bin 0 -> 297 bytes
 .../samtools_split/tool_dependencies.xml      |    1 +
 .../samtools/samtools_stats/.shed.yml         |   12 +
 .../samtools/samtools_stats/macros.xml        |    1 +
 .../samtools_stats/samtools_stats.xml         |  296 +++++
 .../samtools_stats_tool_test_output.html      |  372 ++++++
 .../samtools_stats/test-data/phiX.bam         |  Bin 0 -> 38376 bytes
 .../test-data/samtools_stats_input.bam        |  Bin 0 -> 17406 bytes
 .../test-data/samtools_stats_out1.tab         | 1137 +++++++++++++++++
 .../test-data/samtools_stats_out2.tab         | 1137 +++++++++++++++++
 .../test-data/samtools_stats_out2/gcc.tab     |  252 ++++
 .../test-data/samtools_stats_out2/mpc.tab     |  253 ++++
 .../test-data/samtools_stats_out2/sn.tab      |   33 +
 .../test-data/samtools_stats_ref.fa           |  239 ++++
 .../tool-data/fasta_indexes.loc.sample        |   29 +
 .../tool_data_table_conf.xml.sample           |    7 +
 .../samtools_stats/tool_dependencies.xml      |    1 +
 .../samtools/tool_dependencies.xml            |    6 +
 tool_collections/vcflib/LICENSE               |   21 +
 tool_collections/vcflib/README.rst            |    9 +
 tool_collections/vcflib/macros.xml            |   29 +
 .../vcflib/tool-data/fasta_indexes.loc.sample |   29 +
 .../tool-data/tool_data_table_conf.xml.sample |    8 +
 tool_collections/vcflib/vcf2tsv/.shed.yml     |   14 +
 tool_collections/vcflib/vcf2tsv/macros.xml    |    1 +
 .../vcf2tsv/test-data/vcf2tsv-test1.tab       |   28 +
 .../vcf2tsv/test-data/vcf2tsv-test2.tab       |   13 +
 .../vcflib/vcf2tsv/test-data/vcflib.vcf       |   31 +
 tool_collections/vcflib/vcf2tsv/vcf2tsv.xml   |   45 +
 tool_collections/vcflib/vcfaddinfo/.shed.yml  |   11 +
 tool_collections/vcflib/vcfaddinfo/macros.xml |    1 +
 .../test-data/vcfaddinfo-input1.vcf           |   24 +
 .../vcfaddinfo/test-data/vcfaddinfo-test1.vcf |   25 +
 .../vcflib/vcfaddinfo/test-data/vcflib.vcf    |   31 +
 .../vcflib/vcfaddinfo/vcfaddinfo.xml          |   32 +
 .../vcflib/vcfallelicprimitives/.shed.yml     |   14 +
 .../vcflib/vcfallelicprimitives/macros.xml    |    1 +
 .../test-data/vcfallelicprimitives-test1.vcf  |   35 +
 .../vcfallelicprimitives/test-data/vcflib.vcf |   31 +
 .../vcfallelicprimitives.xml                  |   62 +
 tool_collections/vcflib/vcfannotate/.shed.yml |   11 +
 .../vcflib/vcfannotate/macros.xml             |    1 +
 .../test-data/vcfannotate-test1.vcf           |   32 +
 .../vcfannotate/test-data/vcfannotate.bed     |   46 +
 .../vcflib/vcfannotate/test-data/vcflib.vcf   |   31 +
 .../vcflib/vcfannotate/vcfannotate.xml        |   51 +
 .../vcflib/vcfannotategenotypes/.shed.yml     |   13 +
 .../vcflib/vcfannotategenotypes/macros.xml    |    1 +
 .../test-data/vcfannotategenotypes-input2.vcf |   31 +
 .../test-data/vcfannotategenotypes-test1.vcf  |   33 +
 .../vcfannotategenotypes/test-data/vcflib.vcf |   31 +
 .../vcfannotategenotypes.xml                  |   34 +
 .../vcflib/vcfbedintersect/.shed.yml          |   12 +
 .../vcflib/vcfbedintersect/macros.xml         |    1 +
 .../vcfbedintersect/test-data/vcfannotate.bed |   46 +
 .../test-data/vcfbedintersect-test1.vcf       |   23 +
 .../test-data/vcfbedintersect-test2.vcf       |   24 +
 .../vcfbedintersect/test-data/vcflib.vcf      |   31 +
 .../vcfbedintersect/vcfbedintersect.xml       |   59 +
 .../vcflib/vcfbreakcreatemulti/.shed.yml      |   17 +
 .../vcflib/vcfbreakcreatemulti/macros.xml     |    1 +
 .../test-data/vcfbreakcreatemulti-test1.vcf   |   34 +
 .../vcfbreakcreatemulti-test2-input.vcf       |   34 +
 .../test-data/vcfbreakcreatemulti-test2.vcf   |   32 +
 .../vcfbreakcreatemulti/test-data/vcflib.vcf  |   31 +
 .../vcfbreakcreatemulti.xml                   |   48 +
 tool_collections/vcflib/vcfcheck/.shed.yml    |   10 +
 tool_collections/vcflib/vcfcheck/macros.xml   |    1 +
 .../vcfcheck/test-data/vcfcheck-test1.vcf     |  219 ++++
 .../vcflib/vcfcheck/test-data/vcflib-phix.vcf |  219 ++++
 .../test-data/vcflib-test-genome-phix.fa      |    2 +
 .../tool-data/fasta_indexes.loc.sample        |    1 +
 .../tool-data/tool_data_table_conf.xml.sample |    1 +
 tool_collections/vcflib/vcfcheck/vcfcheck.xml |   74 ++
 tool_collections/vcflib/vcfcombine/.shed.yml  |   14 +
 tool_collections/vcflib/vcfcombine/macros.xml |    1 +
 .../vcfcombine/test-data/vcfcombine-test1.vcf |   31 +
 .../vcflib/vcfcombine/test-data/vcflib.vcf    |   31 +
 .../vcflib/vcfcombine/vcfcombine.xml          |   39 +
 .../vcflib/vcfcommonsamples/.shed.yml         |   10 +
 .../vcflib/vcfcommonsamples/macros.xml        |    1 +
 .../vcfcommonsamples-test1-input2.vcf         |   31 +
 .../test-data/vcfcommonsamples-test1.vcf      |   31 +
 .../vcfcommonsamples/test-data/vcflib.vcf     |   31 +
 .../vcfcommonsamples/vcfcommonsamples.xml     |   32 +
 tool_collections/vcflib/vcfdistance/.shed.yml |   14 +
 .../vcflib/vcfdistance/macros.xml             |    1 +
 .../test-data/vcfdistance-test1.vcf           |   32 +
 .../vcflib/vcfdistance/test-data/vcflib.vcf   |   31 +
 .../vcflib/vcfdistance/vcfdistance.xml        |   34 +
 tool_collections/vcflib/vcffilter/.shed.yml   |   11 +
 tool_collections/vcflib/vcffilter/macros.xml  |    1 +
 .../vcffilter/test-data/vcffilter-test1.vcf   |   26 +
 .../vcflib/vcffilter/test-data/vcflib.vcf     |   31 +
 .../vcflib/vcffilter/vcffilter.xml            |   76 ++
 tool_collections/vcflib/vcffixup/.shed.yml    |   11 +
 tool_collections/vcflib/vcffixup/macros.xml   |    1 +
 .../vcffixup/test-data/vcffixup-test1.vcf     |   31 +
 .../vcflib/vcffixup/test-data/vcflib.vcf      |   31 +
 tool_collections/vcflib/vcffixup/vcffixup.xml |   30 +
 tool_collections/vcflib/vcfflatten/.shed.yml  |   12 +
 tool_collections/vcflib/vcfflatten/macros.xml |    1 +
 .../test-data/vcfflatten-input1.vcf           |  121 ++
 .../vcfflatten/test-data/vcfflatten-test1.vcf |  121 ++
 .../vcflib/vcfflatten/vcfflatten.xml          |   30 +
 .../vcflib/vcfgeno2haplo/.shed.yml            |   12 +
 .../vcflib/vcfgeno2haplo/macros.xml           |    1 +
 .../test-data/vcfgeno2haplo-test1.vcf         |   12 +
 .../vcfgeno2haplo/test-data/vcflib-phix.vcf   |  219 ++++
 .../test-data/vcflib-test-genome-phix.fa      |    2 +
 .../tool-data/fasta_indexes.loc.sample        |    1 +
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diff --git a/tool_collections/kraken/README.rst b/tool_collections/kraken/README.rst
new file mode 100644
index 00000000000..4acd6859923
--- /dev/null
+++ b/tool_collections/kraken/README.rst
@@ -0,0 +1,92 @@
+Introduction
+============
+
+`Kraken <http://ccb.jhu.edu/software/kraken/>`__ is a taxonomic sequence
+classifier that assigns taxonomic labels to short DNA reads. It does
+this by examining the :math:`k`-mers within a read and querying a
+database with those :math:`k`-mers. This database contains a mapping of
+every :math:`k`-mer in
+`Kraken <http://ccb.jhu.edu/software/kraken/>`__'s genomic library to
+the lowest common ancestor (LCA) in a taxonomic tree of all genomes that
+contain that :math:`k`-mer. The set of LCA taxa that correspond to the
+:math:`k`-mers in a read are then analyzed to create a single taxonomic
+label for the read; this label can be any of the nodes in the taxonomic
+tree. `Kraken <http://ccb.jhu.edu/software/kraken/>`__ is designed to be
+rapid, sensitive, and highly precise. Our tests on various real and
+simulated data have shown
+`Kraken <http://ccb.jhu.edu/software/kraken/>`__ to have sensitivity
+slightly lower than Megablast with precision being slightly higher. On a
+set of simulated 100 bp reads,
+`Kraken <http://ccb.jhu.edu/software/kraken/>`__ processed over 1.3
+million reads per minute on a single core in normal operation, and over
+4.1 million reads per minute in quick operation.
+
+The latest released version of Kraken will be available at the `Kraken
+website <http://ccb.jhu.edu/software/kraken/>`__, and the latest updates
+to the Kraken source code are available at the `Kraken GitHub
+repository <https://github.com/DerrickWood/kraken>`__.
+
+If you use `Kraken <http://ccb.jhu.edu/software/kraken/>`__ in your
+research, please cite the `Kraken
+paper <http://genomebiology.com/2014/15/3/R46>`__. Thank you!
+
+System Requirements
+===================
+
+Note: Users concerned about the disk or memory requirements should read
+the paragraph about MiniKraken, below.
+
+-  **Disk space**: Construction of Kraken's standard database will
+   require at least 160 GB of disk space. Customized databases may
+   require more or less space. Disk space used is linearly proportional
+   to the number of distinct :math:`k`-mers; as of Feb. 2015, Kraken's
+   default database contains just under 6 billion (6e9) distinct
+   :math:`k`-mers.
+
+   In addition, the disk used to store the database should be
+   locally-attached storage. Storing the database on a network
+   filesystem (NFS) partition can cause Kraken's operation to be very
+   slow, or to be stopped completely. As NFS accesses are much slower
+   than local disk accesses, both preloading and database building will
+   be slowed by use of NFS.
+
+-  **Memory**: To run efficiently, Kraken requires enough free memory to
+   hold the database in RAM. While this can be accomplished using a
+   ramdisk, Kraken supplies a utility for loading the database into RAM
+   via the OS cache. The default database size is 75 GB (as of Feb.
+   2015), and so you will need at least that much RAM if you want to
+   build or run with the default database.
+
+-  **Dependencies**: Kraken currently makes extensive use of Linux
+   utilities such as sed, find, and wget. Many scripts are written using
+   the Bash shell, and the main scripts are written using Perl. Core
+   programs needed to build the database and run the classifier are
+   written in C++, and need to be compiled using g++. Multithreading is
+   handled using OpenMP. Downloads of NCBI data are performed by wget
+   and in some cases, by rsync. Most Linux systems that have any sort of
+   development package installed will have all of the above listed
+   programs and libraries available.
+
+   Finally, if you want to build your own database, you will need to
+   install the
+   `Jellyfish <http://www.cbcb.umd.edu/software/jellyfish/>`__
+   :math:`k`-mer counter. Note that Kraken only supports use of
+   Jellyfish version 1. Jellyfish version 2 is not yet compatible with
+   Kraken.
+
+-  **Network connectivity**: Kraken's standard database build and
+   download commands expect unfettered FTP and rsync access to the NCBI
+   FTP server. If you're working behind a proxy, you may need to set
+   certain environment variables (such as ``ftp_proxy`` or
+   ``RSYNC_PROXY``) in order to get these commands to work properly.
+
+-  **MiniKraken**: To allow users with low-memory computing environments
+   to use Kraken, we supply a reduced standard database that can be
+   downloaded from the Kraken web site. When Kraken is run with a
+   reduced database, we call it MiniKraken.
+
+   The database we make available is only 4 GB in size, and should run
+   well on computers with as little as 8 GB of RAM. Disk space required
+   for this database is also only 4 GB.
+
+
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diff --git a/tool_collections/kraken/kraken/.shed.yml b/tool_collections/kraken/kraken/.shed.yml
new file mode 100644
index 00000000000..609a78b7e9b
--- /dev/null
+++ b/tool_collections/kraken/kraken/.shed.yml
@@ -0,0 +1,16 @@
+categories:
+- Metagenomics
+description: Kraken for taxonomic designation.
+homepage_url: http://ccb.jhu.edu/software/kraken/
+long_description: |
+  Kraken is a system for assigning taxonomic labels to short DNA
+  sequences, usually obtained through metagenomic studies. Previous attempts by other
+  bioinformatics software to accomplish this task have often used sequence alignment
+  or machine learning techniques that were quite slow, leading to the development
+  of less sensitive but much faster abundance estimation programs. Kraken aims to
+  achieve high sensitivity and high speed by utilizing exact alignments of k-mers
+  and a novel classification algorithm.
+name: kraken
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/blob/master/tool_collections/kraken/kraken/
+type: unrestricted
diff --git a/tool_collections/kraken/kraken/README.rst b/tool_collections/kraken/kraken/README.rst
new file mode 120000
index 00000000000..89a0106941f
--- /dev/null
+++ b/tool_collections/kraken/kraken/README.rst
@@ -0,0 +1 @@
+../README.rst
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken/kraken.xml b/tool_collections/kraken/kraken/kraken.xml
new file mode 100644
index 00000000000..6064c451809
--- /dev/null
+++ b/tool_collections/kraken/kraken/kraken.xml
@@ -0,0 +1,164 @@
+<?xml version="1.0"?>
+<tool id="kraken" name="Kraken" version="1.2.1">
+    <description>
+        assign taxonomic labels to sequencing reads
+    </description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements" />
+    <expand macro="stdio" />
+    <expand macro="version_command" />
+    <command>
+        <![CDATA[
+        @SET_DATABASE_PATH@ &&
+        kraken --threads \${GALAXY_SLOTS:-1} @INPUT_DATABASE@ 
+        
+        ${only_classified_output}
+
+        #if str( $quick_operation.quick ) == "yes":
+            --quick
+            --min-hits ${quick_operation.min_hits}
+
+        #end if
+
+        #if $single_paired.single_paired_selector == "yes":
+            #if $forward_input.is_of_type( 'fastq' ):
+                --fastq-input
+            #else:
+                --fasta-input
+            #end if
+            "$forward_input" "$reverse_input"
+            ${single_paired.check_names}
+        #elif $single_paired.single_paired_selector == "collection":
+            #if $single_paired.input_pair.forward.is_of_type( 'fastq' ):
+                --fastq-input
+            #else:
+                --fasta-input
+            #end if
+            "${single_paired.input_pair.forward}" "${single_paired.input_pair.reverse}"
+            ${single_paired.check_names}
+        #else:
+            #if $input_sequences.is_of_type( 'fastq' ):
+                --fastq-input
+            #else:
+                --fasta-input
+            #end if
+            "$input_sequences"
+        #end if
+
+        #if $split_reads:
+            --classified-out "${classified_out}" --unclassified-out "${unclassified_out}"
+        #end if
+
+        ## The --output option was changed to redirect as it does not work properly is some situations. For example, on test database the tool classifies 4 reads but does not write them into a file if --output is specified. It does however print correct output into STDOUT. This behavior can be re-created with test database provided in test-data/test_db/ folder. This is the reason for incrementing version number from 1.1.2 to 1.1.3
+
+        > "${output}"
+        ##kraken-translate --db ${kraken_database.fields.name} "${output}" > "${translated}"
+        ]]>
+    </command>
+    <inputs>
+        <conditional name="single_paired">
+            <param name="single_paired_selector" type="select" label="Single or paired reads" help="--paired">
+                <option value="collection">Collection</option>
+                <option value="yes">Paired</option>
+                <option selected="True" value="no">Single</option>
+            </param>
+            <when value="collection">
+                <param format="fasta,fastq" label="Collection of paired reads" name="input_pair" type="data_collection" collection_type="paired" help="FASTA or FASTQ datasets" />
+                <param name="check_names" type="boolean" checked="False" truevalue="--paired --check-names" falsevalue="--paired" label="Verify read names match" help="--check-names" />
+            </when>
+            <when value="yes">
+                <param format="fasta,fastq" label="Forward strand" name="forward_input" type="data" help="FASTA or FASTQ dataset"/>
+                <param format="fasta,fastq" label="Reverse strand" name="reverse_input" type="data" help="FASTA or FASTQ dataset"/>
+                <param name="check_names" type="boolean" checked="False" truevalue="--paired --check-names" falsevalue="--paired" label="Verify read names match" help="--check-names" />
+            </when>
+            <when value="no">
+                <param format="fasta,fastq" label="Input sequences" name="input_sequences" type="data" help="FASTA or FASTQ datasets"/>
+            </when>
+
+        </conditional>
+        <param label="Output classified and unclassified reads?" name="split_reads" type="boolean" help="Sets --unclassified-out and --classified-out"/>
+  
+        <conditional name="quick_operation">
+            <param name="quick" type="select" label="Enable quick operation?" help="--quick; Rather than searching all k-mers in a sequence, stop classification after a specified number of database hit">
+                <option value="yes">Yes</option>
+                <option selected="True" value="no">No</option>
+            </param>
+            <when value="yes">
+                <param name="min_hits" type="integer" value="1" label="Number of hits required for classification" help="--min-hits; min-hits will allow you to require multiple hits before declaring a sequence classified, which can be especially useful with custom databases when testing to see if sequences either do or do not belong to a particular genome; default=1"/>
+            </when>
+            <when value="no">
+                <!-- Do absolutely nothing -->
+            </when>
+        </conditional>
+
+        <param name="only_classified_output" type="boolean" checked="False" truevalue="--only-classified-output" falsevalue="" label="Print no Kraken output for unclassified sequences" help="--only-classified-output"/>
+
+        <expand macro="input_database" />
+    </inputs>
+    <outputs>
+        <data format_source="input_sequences" label="${tool.name} on ${on_string}: Classified reads" name="classified_out">
+            <filter>(split_reads)</filter>
+        </data>
+        <data format_source="input_sequences" label="${tool.name} on ${on_string}: Unclassified reads" name="unclassified_out">
+            <filter>(split_reads)</filter>
+        </data>
+        <data format="tabular" label="${tool.name} on ${on_string}: Classification" name="output" />
+        <!--<data format="tabular" label="${tool.name} on ${on_string}: Translated classification" name="translated" />-->
+    </outputs>
+
+    <tests>
+        <test>
+            <param name="single_paired_selector" value="no"/>
+            <param name="input_sequences" value="kraken_test1.fa" ftype="fasta"/>
+            <param name="split_reads" value="false"/>
+            <param name="quick" value="no"/>
+            <param name="only-classified-output" value="false"/>
+            <param name="kraken_database" value="test_db"/>
+            <output name="output" file="kraken_test1_output.tab" ftype="tabular"/>
+        </test>
+    </tests>
+    <help>
+        <![CDATA[
+**What it does**
+
+Kraken is a taxonomic sequence classifier that assigns taxonomic labels to short DNA reads. It does this by examining the k-mers within a read and querying a database with those k-mers. This database contains a mapping of every k-mer in Kraken's genomic library to the lowest common ancestor (LCA) in a taxonomic tree of all genomes that contain that k-mer. The set of LCA taxa that correspond to the k-mers in a read are then analyzed to create a single taxonomic label for the read; this label can be any of the nodes in the taxonomic tree. Kraken is designed to be rapid, sensitive, and highly precise.
+
+-----
+
+**Kraken options**
+
+The Galaxy version of Kraken implements the following options::
+
+        
+  --fasta-input             Input is FASTA format
+  --fastq-input             Input is FASTQ format
+  --quick                   Quick operation (use first hit or hits)
+  --min-hits NUM            In quick op., number of hits req'd for classification
+                            NOTE: this is ignored if --quick is not specified
+  --unclassified-out        Print unclassified sequences to filename
+  --classified-out          Print classified sequences to filename
+
+  --only-classified-output  Print no Kraken output for unclassified sequences
+                          
+------
+
+**Output Format**
+
+Each sequence classified by Kraken results in a single line of output. Output lines contain five tab-delimited fields; from left to right, they are::
+
+    1. "C"/"U": one letter code indicating that the sequence was either classified or unclassified.
+    2. The sequence ID, obtained from the FASTA/FASTQ header.
+    3. The taxonomy ID Kraken used to label the sequence; this is 0 if the sequence is unclassified.
+    4. The length of the sequence in bp.
+    5. A space-delimited list indicating the LCA mapping of each k-mer in the sequence. For example, "562:13 561:4 A:31 0:1 562:3" would indicate that:
+            a) the first 13 k-mers mapped to taxonomy ID #562
+            b) the next 4 k-mers mapped to taxonomy ID #561
+            c) the next 31 k-mers contained an ambiguous nucleotide
+            d) the next k-mer was not in the database
+            e) the last 3 k-mers mapped to taxonomy ID #562
+        ]]>
+    </help>
+    <expand macro="citations" />
+</tool>
diff --git a/tool_collections/kraken/kraken/macros.xml b/tool_collections/kraken/kraken/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/kraken/kraken/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken/test-data/kraken_test1.fa b/tool_collections/kraken/kraken/test-data/kraken_test1.fa
new file mode 100644
index 00000000000..dfc5125a6a6
--- /dev/null
+++ b/tool_collections/kraken/kraken/test-data/kraken_test1.fa
@@ -0,0 +1,70 @@
+>gi|145231|gb|M33724.1|ECOALPHOA Escherichia coli K-12 truncated PhoA (phoA) gene, partial cds; and transposon Mu dI, partial sequence
+CAAAGCTCCGGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAAC
+TCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCA
+ATGAAGCGGCGCACGAAAAACGCGAAAGCGT
+
+>gi|145232|gb|M33725.1|ECOALPHOB Escherichia coli K12 phoA pseudogene and transposon Mu dl-R, partial sequence
+CTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCTTTGTTTTTATTTTTTAATGTATTTGTACATGG
+AGAAAATAAAGTGAAACAAAGCACTATTGCACTGGCACTCTTACCGTTACTGTTTACCCCTGTGACAAAA
+GCCCGGACACCAGTGAAGCGGCGCACGAAAAACGCGAAAGCGT
+
+>gi|145234|gb|M33727.1|ECOALPHOE Escherichia coli K12 upstream sequence of psiA5::Mu dI. is identical to psiA30 upstream sequence; putative (phoA) pseudogene and transposon Mu dl-R, partial sequence
+TTGTTTTTATTTTTTAATGTATTTGTACATGGAGAAAATAAAGTGAAACAAAGCACTATTGCACTGGTGA
+AGCGGCGCACGAAAAACGCGAAAGCGT
+
+>gi|146195|gb|J01619.1|ECOGLTA Eschericia coli gltA gene, sdhCDAB operon and sucABCD operons, complete sequence
+GAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTC
+ACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCAAAATAATACTTATTTATAA
+TCCAGCACGTAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCA
+TCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACC
+TAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTG
+TTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAAACTTTGGATACC
+GGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTAT
+TGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTC
+TGGATGAATTTCACAAGGTGCTTCAATAACCTCCCCCTTAAAGTGAATTTCGCCAGAACCTTCATCAGCA
+GCATAAACAGGTGCAGTGAACAGCAGAGATACGGCCAGTGCGGCCAATGTTTTTTGTCCTTTAAACATAA
+CAGAGTCCTTTAAGGATATAGAATAGGGGTATAGCTACGCCAGAATATCGTATTTGATTATTGCTAGTTT
+TTAGTTTTGCTTAAAAAATATTGTTAGTTTTATTAAATTGGAAAACTAAATTATTGGTATCATGAATTGT
+TGTATGATGATAAATATAGGGGGGATATGATAGACGTCATTTTCATAGGGTTATAAAATGCGACTACCAT
+GAAGTTTTTAATTCAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATA
+GGTCTGTTAATGGATTTTATTTTTACAAGTTTTTTGTGTTTAGGCATATAAAAATCAAGCCCGCCATATG
+AACGGCGGGTTAAAATATTTACAACTTAGCAATCGAACCATTAACGCTTGATATCGCTTTTAAAGTCGCG
+TTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAG
+TGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCG
+CTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTT
+CAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACT
+TCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCA
+TCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAAAAAATTCCGGAATGTGTTTAACGGAGCTGAT
+TTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCT
+GCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCT
+GTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGG
+TTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACA
+AATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACG
+CGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGT
+AATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTCG
+TGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCAT
+TCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTG
+CAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCT
+GGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTT
+TCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGG
+TCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTAC
+CCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCA
+GGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTC
+TACCACTAATAACTGTCCCGAATGAATTGGTCAATACTCCACACTGTTACATAAGTTAATCTTAGGTGAA
+ATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGA
+TTGTCAAATTAGATGATTAAAAATTAAATAAATGTTGTTATCGTGACCTGGATCACTGTTCAGGATAAAA
+CCCGACAAACTATATGTAGGTTAATTGTAATGATTTTGTGAACAGCCTATACTGCCGCCAGTCTCCGGAA
+CACCCTGCAATCCCGAGCCACCCAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATG
+ACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCA
+GGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTC
+ATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGG
+CGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCT
+TCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTC
+AAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGA
+TGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTAC
+TGTCGTGCTTTCACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCG
+TACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTACATCATTTATATGGTCGGTTT
+TTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTG
+TTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACT
+ACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGAT
+TTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCAGTCAGAGAATTTGATGCAGTTGTGATTG
diff --git a/tool_collections/kraken/kraken/test-data/kraken_test1_output.tab b/tool_collections/kraken/kraken/test-data/kraken_test1_output.tab
new file mode 100644
index 00000000000..2aa9e1ea8d5
--- /dev/null
+++ b/tool_collections/kraken/kraken/test-data/kraken_test1_output.tab
@@ -0,0 +1,4 @@
+C	gi|145231|gb|M33724.1|ECOALPHOA	83333	171	83333:162
+C	gi|145232|gb|M33725.1|ECOALPHOB	83333	183	83333:174
+C	gi|145234|gb|M33727.1|ECOALPHOE	83333	97	83333:88
+C	gi|146195|gb|J01619.1|ECOGLTA	83333	3850	83333:3841
diff --git a/tool_collections/kraken/kraken/test-data/test_database.loc b/tool_collections/kraken/kraken/test-data/test_database.loc
new file mode 120000
index 00000000000..03ec4336e45
--- /dev/null
+++ b/tool_collections/kraken/kraken/test-data/test_database.loc
@@ -0,0 +1 @@
+../../test_database.loc
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken/test-data/test_db/database.idx b/tool_collections/kraken/kraken/test-data/test_db/database.idx
new file mode 120000
index 00000000000..9251273d383
--- /dev/null
+++ b/tool_collections/kraken/kraken/test-data/test_db/database.idx
@@ -0,0 +1 @@
+../../../database.idx
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken/test-data/test_db/database.kdb b/tool_collections/kraken/kraken/test-data/test_db/database.kdb
new file mode 120000
index 00000000000..64efac5ce08
--- /dev/null
+++ b/tool_collections/kraken/kraken/test-data/test_db/database.kdb
@@ -0,0 +1 @@
+../../../database.kdb
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken/test-data/test_db/taxonomy/names.dmp b/tool_collections/kraken/kraken/test-data/test_db/taxonomy/names.dmp
new file mode 120000
index 00000000000..f4585471fc2
--- /dev/null
+++ b/tool_collections/kraken/kraken/test-data/test_db/taxonomy/names.dmp
@@ -0,0 +1 @@
+../../../../names.dmp
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken/test-data/test_db/taxonomy/nodes.dmp b/tool_collections/kraken/kraken/test-data/test_db/taxonomy/nodes.dmp
new file mode 120000
index 00000000000..a65d7d41f87
--- /dev/null
+++ b/tool_collections/kraken/kraken/test-data/test_db/taxonomy/nodes.dmp
@@ -0,0 +1 @@
+../../../../nodes.dmp
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken/tool-data/kraken_databases.loc.sample b/tool_collections/kraken/kraken/tool-data/kraken_databases.loc.sample
new file mode 120000
index 00000000000..eba8983e9ea
--- /dev/null
+++ b/tool_collections/kraken/kraken/tool-data/kraken_databases.loc.sample
@@ -0,0 +1 @@
+../../kraken_databases.loc.sample
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken/tool_data_table_conf.xml.sample b/tool_collections/kraken/kraken/tool_data_table_conf.xml.sample
new file mode 120000
index 00000000000..332baf440e9
--- /dev/null
+++ b/tool_collections/kraken/kraken/tool_data_table_conf.xml.sample
@@ -0,0 +1 @@
+../tool_data_table_conf.xml.sample
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken/tool_data_table_conf.xml.test b/tool_collections/kraken/kraken/tool_data_table_conf.xml.test
new file mode 120000
index 00000000000..f8050b61701
--- /dev/null
+++ b/tool_collections/kraken/kraken/tool_data_table_conf.xml.test
@@ -0,0 +1 @@
+../tool_data_table_conf.xml.test
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken/tool_dependencies.xml b/tool_collections/kraken/kraken/tool_dependencies.xml
new file mode 100644
index 00000000000..04f55a93faf
--- /dev/null
+++ b/tool_collections/kraken/kraken/tool_dependencies.xml
@@ -0,0 +1,6 @@
+<?xml version="1.0"?>
+<tool_dependency>
+  <package name="kraken" version="0.10.6-eaf8fb68">
+      <repository name="package_kraken_0_10_6_eaf8fb68" owner="iuc" />
+    </package>
+</tool_dependency>
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_databases.loc.sample b/tool_collections/kraken/kraken_databases.loc.sample
new file mode 100644
index 00000000000..e69de29bb2d
diff --git a/tool_collections/kraken/kraken_filter/.shed.yml b/tool_collections/kraken/kraken_filter/.shed.yml
new file mode 100644
index 00000000000..89bc8f6636d
--- /dev/null
+++ b/tool_collections/kraken/kraken_filter/.shed.yml
@@ -0,0 +1,16 @@
+categories:
+- Metagenomics
+description: Kraken's confidence filter.
+homepage_url: http://ccb.jhu.edu/software/kraken/
+long_description: |
+  Kraken is a system for assigning taxonomic labels to short DNA
+  sequences, usually obtained through metagenomic studies. Previous attempts by other
+  bioinformatics software to accomplish this task have often used sequence alignment
+  or machine learning techniques that were quite slow, leading to the development
+  of less sensitive but much faster abundance estimation programs. Kraken aims to
+  achieve high sensitivity and high speed by utilizing exact alignments of k-mers
+  and a novel classification algorithm.
+name: kraken_filter
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/blob/master/tool_collections/kraken/kraken_filter/
+type: unrestricted
diff --git a/tool_collections/kraken/kraken_filter/README.rst b/tool_collections/kraken/kraken_filter/README.rst
new file mode 120000
index 00000000000..89a0106941f
--- /dev/null
+++ b/tool_collections/kraken/kraken_filter/README.rst
@@ -0,0 +1 @@
+../README.rst
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_filter/kraken-filter.xml b/tool_collections/kraken/kraken_filter/kraken-filter.xml
new file mode 100644
index 00000000000..99206efcbb7
--- /dev/null
+++ b/tool_collections/kraken/kraken_filter/kraken-filter.xml
@@ -0,0 +1,63 @@
+<tool id="kraken-filter" name="Kraken-filter" version="1.2.1">
+    <description>
+        filter classification by confidence score
+    </description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements" />
+    <expand macro="stdio" />
+    <expand macro="version_command" />
+    <command>
+        <![CDATA[
+        @SET_DATABASE_PATH@ &&
+        kraken-filter @INPUT_DATABASE@ --threshold $threshold "${input}" > "$filtered_output"
+        ]]>
+    </command>
+    <inputs>
+        <param format="tabular" label="Kraken output" name="input" type="data" help="Select taxonomy classification produced by kraken"/>
+        <param label="Confidence threshold" max="1" min="0" name="threshold" type="float" value="0" help="--threshold; A number between 0 and 1; default=0"/>
+        <expand macro="input_database" />
+    </inputs>
+    <outputs>
+        <data format="tabular" name="filtered_output" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="input" value="kraken_filter_test1.tab"/>
+            <param name="threshold" value="0"/>
+            <param name="kraken_database" value="test_db"/>
+            <output name="output" file="kraken_filter_test1_output.tab" ftype="tabular"/>
+        </test>
+    </tests>
+
+    <help>
+<![CDATA[
+
+.. class:: warningmark
+
+**Note**: the database used must be the same as the one used in the original Kraken run
+
+-----
+
+**What it does**
+
+At present, we have not yet developed a confidence score with a solid probabilistic interpretation for Kraken. However, we have developed a simple scoring scheme that has yielded good results for us, and we've made that available in the kraken-filter script. The approach we use allows a user to specify a threshold score in the [0,1] interval; the ``kraken-filter`` script then will adjust labels up the tree until the label's score (described below) meets or exceeds that threshold. If a label at the root of the taxonomic tree would not have a score exceeding the threshold, the sequence is called unclassified by ``kraken-filter``.
+
+A sequence label's score is a fraction C/Q, where C is the number of k-mers mapped to LCA values in the clade rooted at the label, and Q is the number of k-mers in the sequence that lack an ambiguous nucleotide (i.e., they were queried against the database). Consider the example of the LCA mappings in Kraken's output::
+
+ 562:13 561:4 A:31 0:1 562:3 
+
+would indicate that::
+
+ the first 13 k-mers mapped to taxonomy ID #562
+ the next 4 k-mers mapped to taxonomy ID #561
+ the next 31 k-mers contained an ambiguous nucleotide
+ the next k-mer was not in the database
+ the last 3 k-mers mapped to taxonomy ID #562
+
+In this case, ID #561 is the parent node of #562. Here, a label of #562 for this sequence would have a score of C/Q = (13+3)/(13+4+1+3) = 16/21. A label of #561 would have a score of C/Q = (13+4+3)/(13+4+1+3) = 20/21. If a user specified a threshold over 16/21, kraken-filter would adjust the original label from #562 to #561; if the threshold was greater than 20/21, the sequence would become unclassified.
+    ]]>
+    </help>
+    <expand macro="citations" />
+</tool>
diff --git a/tool_collections/kraken/kraken_filter/macros.xml b/tool_collections/kraken/kraken_filter/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/kraken/kraken_filter/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_filter/test-data/kraken_filter_test1.tab b/tool_collections/kraken/kraken_filter/test-data/kraken_filter_test1.tab
new file mode 100644
index 00000000000..2aa9e1ea8d5
--- /dev/null
+++ b/tool_collections/kraken/kraken_filter/test-data/kraken_filter_test1.tab
@@ -0,0 +1,4 @@
+C	gi|145231|gb|M33724.1|ECOALPHOA	83333	171	83333:162
+C	gi|145232|gb|M33725.1|ECOALPHOB	83333	183	83333:174
+C	gi|145234|gb|M33727.1|ECOALPHOE	83333	97	83333:88
+C	gi|146195|gb|J01619.1|ECOGLTA	83333	3850	83333:3841
diff --git a/tool_collections/kraken/kraken_filter/test-data/kraken_filter_test1_output.tab b/tool_collections/kraken/kraken_filter/test-data/kraken_filter_test1_output.tab
new file mode 100644
index 00000000000..9050d543951
--- /dev/null
+++ b/tool_collections/kraken/kraken_filter/test-data/kraken_filter_test1_output.tab
@@ -0,0 +1,4 @@
+C	gi|145231|gb|M33724.1|ECOALPHOA	83333	171	P=1.000	83333:162
+C	gi|145232|gb|M33725.1|ECOALPHOB	83333	183	P=1.000	83333:174
+C	gi|145234|gb|M33727.1|ECOALPHOE	83333	97	P=1.000	83333:88
+C	gi|146195|gb|J01619.1|ECOGLTA	83333	3850	P=1.000	83333:3841
diff --git a/tool_collections/kraken/kraken_filter/test-data/kraken_test1.fa b/tool_collections/kraken/kraken_filter/test-data/kraken_test1.fa
new file mode 100644
index 00000000000..dfc5125a6a6
--- /dev/null
+++ b/tool_collections/kraken/kraken_filter/test-data/kraken_test1.fa
@@ -0,0 +1,70 @@
+>gi|145231|gb|M33724.1|ECOALPHOA Escherichia coli K-12 truncated PhoA (phoA) gene, partial cds; and transposon Mu dI, partial sequence
+CAAAGCTCCGGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAAC
+TCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCA
+ATGAAGCGGCGCACGAAAAACGCGAAAGCGT
+
+>gi|145232|gb|M33725.1|ECOALPHOB Escherichia coli K12 phoA pseudogene and transposon Mu dl-R, partial sequence
+CTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCTTTGTTTTTATTTTTTAATGTATTTGTACATGG
+AGAAAATAAAGTGAAACAAAGCACTATTGCACTGGCACTCTTACCGTTACTGTTTACCCCTGTGACAAAA
+GCCCGGACACCAGTGAAGCGGCGCACGAAAAACGCGAAAGCGT
+
+>gi|145234|gb|M33727.1|ECOALPHOE Escherichia coli K12 upstream sequence of psiA5::Mu dI. is identical to psiA30 upstream sequence; putative (phoA) pseudogene and transposon Mu dl-R, partial sequence
+TTGTTTTTATTTTTTAATGTATTTGTACATGGAGAAAATAAAGTGAAACAAAGCACTATTGCACTGGTGA
+AGCGGCGCACGAAAAACGCGAAAGCGT
+
+>gi|146195|gb|J01619.1|ECOGLTA Eschericia coli gltA gene, sdhCDAB operon and sucABCD operons, complete sequence
+GAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTC
+ACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCAAAATAATACTTATTTATAA
+TCCAGCACGTAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCA
+TCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACC
+TAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTG
+TTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAAACTTTGGATACC
+GGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTAT
+TGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTC
+TGGATGAATTTCACAAGGTGCTTCAATAACCTCCCCCTTAAAGTGAATTTCGCCAGAACCTTCATCAGCA
+GCATAAACAGGTGCAGTGAACAGCAGAGATACGGCCAGTGCGGCCAATGTTTTTTGTCCTTTAAACATAA
+CAGAGTCCTTTAAGGATATAGAATAGGGGTATAGCTACGCCAGAATATCGTATTTGATTATTGCTAGTTT
+TTAGTTTTGCTTAAAAAATATTGTTAGTTTTATTAAATTGGAAAACTAAATTATTGGTATCATGAATTGT
+TGTATGATGATAAATATAGGGGGGATATGATAGACGTCATTTTCATAGGGTTATAAAATGCGACTACCAT
+GAAGTTTTTAATTCAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATA
+GGTCTGTTAATGGATTTTATTTTTACAAGTTTTTTGTGTTTAGGCATATAAAAATCAAGCCCGCCATATG
+AACGGCGGGTTAAAATATTTACAACTTAGCAATCGAACCATTAACGCTTGATATCGCTTTTAAAGTCGCG
+TTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAG
+TGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCG
+CTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTT
+CAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACT
+TCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCA
+TCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAAAAAATTCCGGAATGTGTTTAACGGAGCTGAT
+TTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCT
+GCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCT
+GTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGG
+TTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACA
+AATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACG
+CGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGT
+AATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTCG
+TGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCAT
+TCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTG
+CAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCT
+GGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTT
+TCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGG
+TCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTAC
+CCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCA
+GGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTC
+TACCACTAATAACTGTCCCGAATGAATTGGTCAATACTCCACACTGTTACATAAGTTAATCTTAGGTGAA
+ATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGA
+TTGTCAAATTAGATGATTAAAAATTAAATAAATGTTGTTATCGTGACCTGGATCACTGTTCAGGATAAAA
+CCCGACAAACTATATGTAGGTTAATTGTAATGATTTTGTGAACAGCCTATACTGCCGCCAGTCTCCGGAA
+CACCCTGCAATCCCGAGCCACCCAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATG
+ACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCA
+GGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTC
+ATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGG
+CGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCT
+TCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTC
+AAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGA
+TGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTAC
+TGTCGTGCTTTCACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCG
+TACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTACATCATTTATATGGTCGGTTT
+TTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTG
+TTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACT
+ACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGAT
+TTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCAGTCAGAGAATTTGATGCAGTTGTGATTG
diff --git a/tool_collections/kraken/kraken_filter/test-data/test_database.loc b/tool_collections/kraken/kraken_filter/test-data/test_database.loc
new file mode 120000
index 00000000000..03ec4336e45
--- /dev/null
+++ b/tool_collections/kraken/kraken_filter/test-data/test_database.loc
@@ -0,0 +1 @@
+../../test_database.loc
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_filter/test-data/test_db/database.idx b/tool_collections/kraken/kraken_filter/test-data/test_db/database.idx
new file mode 120000
index 00000000000..9251273d383
--- /dev/null
+++ b/tool_collections/kraken/kraken_filter/test-data/test_db/database.idx
@@ -0,0 +1 @@
+../../../database.idx
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_filter/test-data/test_db/database.kdb b/tool_collections/kraken/kraken_filter/test-data/test_db/database.kdb
new file mode 120000
index 00000000000..64efac5ce08
--- /dev/null
+++ b/tool_collections/kraken/kraken_filter/test-data/test_db/database.kdb
@@ -0,0 +1 @@
+../../../database.kdb
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_filter/test-data/test_db/taxonomy/names.dmp b/tool_collections/kraken/kraken_filter/test-data/test_db/taxonomy/names.dmp
new file mode 120000
index 00000000000..f4585471fc2
--- /dev/null
+++ b/tool_collections/kraken/kraken_filter/test-data/test_db/taxonomy/names.dmp
@@ -0,0 +1 @@
+../../../../names.dmp
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_filter/test-data/test_db/taxonomy/nodes.dmp b/tool_collections/kraken/kraken_filter/test-data/test_db/taxonomy/nodes.dmp
new file mode 120000
index 00000000000..a65d7d41f87
--- /dev/null
+++ b/tool_collections/kraken/kraken_filter/test-data/test_db/taxonomy/nodes.dmp
@@ -0,0 +1 @@
+../../../../nodes.dmp
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_filter/tool-data/kraken_databases.loc.sample b/tool_collections/kraken/kraken_filter/tool-data/kraken_databases.loc.sample
new file mode 120000
index 00000000000..eba8983e9ea
--- /dev/null
+++ b/tool_collections/kraken/kraken_filter/tool-data/kraken_databases.loc.sample
@@ -0,0 +1 @@
+../../kraken_databases.loc.sample
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_filter/tool_data_table_conf.xml.sample b/tool_collections/kraken/kraken_filter/tool_data_table_conf.xml.sample
new file mode 120000
index 00000000000..332baf440e9
--- /dev/null
+++ b/tool_collections/kraken/kraken_filter/tool_data_table_conf.xml.sample
@@ -0,0 +1 @@
+../tool_data_table_conf.xml.sample
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_filter/tool_data_table_conf.xml.test b/tool_collections/kraken/kraken_filter/tool_data_table_conf.xml.test
new file mode 120000
index 00000000000..f8050b61701
--- /dev/null
+++ b/tool_collections/kraken/kraken_filter/tool_data_table_conf.xml.test
@@ -0,0 +1 @@
+../tool_data_table_conf.xml.test
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_filter/tool_dependencies.xml b/tool_collections/kraken/kraken_filter/tool_dependencies.xml
new file mode 100644
index 00000000000..04f55a93faf
--- /dev/null
+++ b/tool_collections/kraken/kraken_filter/tool_dependencies.xml
@@ -0,0 +1,6 @@
+<?xml version="1.0"?>
+<tool_dependency>
+  <package name="kraken" version="0.10.6-eaf8fb68">
+      <repository name="package_kraken_0_10_6_eaf8fb68" owner="iuc" />
+    </package>
+</tool_dependency>
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_report/.shed.yml b/tool_collections/kraken/kraken_report/.shed.yml
new file mode 100644
index 00000000000..2dea471fdf4
--- /dev/null
+++ b/tool_collections/kraken/kraken_report/.shed.yml
@@ -0,0 +1,16 @@
+categories:
+- Metagenomics
+description: Kraken report.
+homepage_url: http://ccb.jhu.edu/software/kraken/
+long_description: |
+  Kraken is a system for assigning taxonomic labels to short DNA
+  sequences, usually obtained through metagenomic studies. Previous attempts by other
+  bioinformatics software to accomplish this task have often used sequence alignment
+  or machine learning techniques that were quite slow, leading to the development
+  of less sensitive but much faster abundance estimation programs. Kraken aims to
+  achieve high sensitivity and high speed by utilizing exact alignments of k-mers
+  and a novel classification algorithm.
+name: kraken_report
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/blob/master/tool_collections/kraken/kraken_report/
+type: unrestricted
diff --git a/tool_collections/kraken/kraken_report/README.rst b/tool_collections/kraken/kraken_report/README.rst
new file mode 120000
index 00000000000..89a0106941f
--- /dev/null
+++ b/tool_collections/kraken/kraken_report/README.rst
@@ -0,0 +1 @@
+../README.rst
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_report/kraken-mpa-report.xml b/tool_collections/kraken/kraken_report/kraken-mpa-report.xml
new file mode 100644
index 00000000000..2a2a57b8413
--- /dev/null
+++ b/tool_collections/kraken/kraken_report/kraken-mpa-report.xml
@@ -0,0 +1,83 @@
+<?xml version="1.0"?>
+<tool id="kraken-mpa-report" name="Kraken-mpa-report" version="1.2.2">
+    <description>view report of classification for multiple samples</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements" />
+    <expand macro="stdio" />
+    <expand macro="version_command" />
+    <command>
+<![CDATA[
+
+#set $names = []
+
+#for $input_count, $input_classification in enumerate( $classification ):
+    #set $name_base = str( getattr( $input_classification, 'element_identifier', 'sample' ) ).replace( "/", '-' ).replace( "\t", "-" )
+    #set $name = $name_base
+    #set $i = 1
+    #while $name in $names:
+        #set $name = "%s_%s" % ( $name_base, $i )
+        #set $i = $i + 1
+    #end while
+    #silent $names.append( $name )
+    ln -s "${input_classification}" "${name}" &&
+#end for
+
+@SET_DATABASE_PATH@ && kraken-mpa-report @INPUT_DATABASE@
+
+#for $name in $names:
+    "${name}"
+#end for
+
+${show_zeros}
+${header_line}
+
+> "$output_report"
+]]>
+    </command>
+    <inputs>
+        <param format="tabular" label="Kraken output" multiple="True" name="classification" type="data" />
+        <param checked="False" falsevalue="" help="--show-zeros" label="Display taxa even if they lack a read in any sample" name="show_zeros" truevalue="--show-zeros" type="boolean" />
+        <param checked="False" falsevalue="" help="--header-line" label="Display a header line indicating sample IDs" name="header_line" truevalue="--header-line" type="boolean" />
+        <expand macro="input_database" />
+    </inputs>
+    <outputs>
+        <data format="tabular" name="output_report" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="classification" value="kraken_mpa_report_input1.tab,kraken_mpa_report_input2.tab" ftype="tabular"/>
+            <param name="show_zeros" value="--show-zeros"/>
+            <param name="header_line" value="--header-line"/>
+            <param name="kraken_database" value="test_db"/>
+            <output name="output_report" file="kraken_mpa_report_test1_output.tab" ftype="tabular"/>
+        </test>
+    </tests>
+    <help>
+<![CDATA[
+
+.. class:: warningmark
+
+**Note**: the database used must be the same as the one used in the original Kraken run
+
+-----
+
+**What is Does**
+
+Kraken-mpa-report summarizes read counts across taxonomic ranks for multiple samples. This is convenient for comparing results across multiple experiments, conditions, locations, etc. It supports the following parameters::
+
+--show-zeros    Display taxa even if they lack a read in any sample
+--header-line   Display a header line indicating sample IDs
+                (sample IDs are the filenames)
+
+-----
+
+**Output**
+
+The output of kraken-mpa-report is tab-delimited, with one line per taxon.
+
+]]>
+    </help>
+    <expand macro="citations" />
+</tool>
diff --git a/tool_collections/kraken/kraken_report/kraken-report.xml b/tool_collections/kraken/kraken_report/kraken-report.xml
new file mode 100644
index 00000000000..c4cc134c408
--- /dev/null
+++ b/tool_collections/kraken/kraken_report/kraken-report.xml
@@ -0,0 +1,58 @@
+<tool id="kraken-report" name="Kraken-report" version="1.2.1">
+    <description>
+        view a sample report of your classification
+    </description>
+    
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements" />
+    <expand macro="stdio" />
+    <expand macro="version_command" />
+    <command>
+        <![CDATA[
+        @SET_DATABASE_PATH@ &&
+        kraken-report @INPUT_DATABASE@ "${kraken_output}" > "$output_report"
+        ]]>
+    </command>
+    <inputs>
+        <param format="tabular" label="Kraken output" name="kraken_output" type="data" help="Select taxonomy classification produced by kraken"/>
+        <expand macro="input_database" />
+    </inputs>
+    <outputs>
+        <data format="tabular" name="output_report" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="kraken_output" value="kraken_report_test1.tab" ftype="tabular"/>
+            <param name="kraken_database" value="test_db"/>
+            <output name="output_report" file="kraken_report_test1_output.tab" ftype="tabular"/>
+        </test>
+    </tests>
+
+    <help>
+<![CDATA[
+
+.. class:: warningmark
+
+**Note**: the database used must be the same as the one used in the original Kraken run
+
+-----
+
+**Output**
+
+The output of kraken-report is tab-delimited, with one line per taxon. The fields of the output, from left-to-right, are as follows::
+
+ 1. Percentage of reads covered by the clade rooted at this taxon
+ 2. Number of reads covered by the clade rooted at this taxon
+ 3. Number of reads assigned directly to this taxon
+ 4. A rank code, indicating (U)nclassified, (D)omain, (K)ingdom, (P)hylum, (C)lass, (O)rder, (F)amily, (G)enus, or (S)pecies. All other ranks are simply filled with a dash.
+ 5. NCBI taxonomy ID
+ 6. Indented scientific name
+
+The scientific names are indented using spaces, according to the tree
+structure specified by the taxonomy.
+    ]]>
+    </help>
+    <expand macro="citations" />
+</tool>
diff --git a/tool_collections/kraken/kraken_report/macros.xml b/tool_collections/kraken/kraken_report/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/kraken/kraken_report/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_report/test-data/kraken_mpa_report_input1.tab b/tool_collections/kraken/kraken_report/test-data/kraken_mpa_report_input1.tab
new file mode 100644
index 00000000000..2aa9e1ea8d5
--- /dev/null
+++ b/tool_collections/kraken/kraken_report/test-data/kraken_mpa_report_input1.tab
@@ -0,0 +1,4 @@
+C	gi|145231|gb|M33724.1|ECOALPHOA	83333	171	83333:162
+C	gi|145232|gb|M33725.1|ECOALPHOB	83333	183	83333:174
+C	gi|145234|gb|M33727.1|ECOALPHOE	83333	97	83333:88
+C	gi|146195|gb|J01619.1|ECOGLTA	83333	3850	83333:3841
diff --git a/tool_collections/kraken/kraken_report/test-data/kraken_mpa_report_input2.tab b/tool_collections/kraken/kraken_report/test-data/kraken_mpa_report_input2.tab
new file mode 100644
index 00000000000..2aa9e1ea8d5
--- /dev/null
+++ b/tool_collections/kraken/kraken_report/test-data/kraken_mpa_report_input2.tab
@@ -0,0 +1,4 @@
+C	gi|145231|gb|M33724.1|ECOALPHOA	83333	171	83333:162
+C	gi|145232|gb|M33725.1|ECOALPHOB	83333	183	83333:174
+C	gi|145234|gb|M33727.1|ECOALPHOE	83333	97	83333:88
+C	gi|146195|gb|J01619.1|ECOGLTA	83333	3850	83333:3841
diff --git a/tool_collections/kraken/kraken_report/test-data/kraken_mpa_report_test1_output.tab b/tool_collections/kraken/kraken_report/test-data/kraken_mpa_report_test1_output.tab
new file mode 100644
index 00000000000..ac03dc264ef
--- /dev/null
+++ b/tool_collections/kraken/kraken_report/test-data/kraken_mpa_report_test1_output.tab
@@ -0,0 +1,8 @@
+#Sample ID	kraken_mpa_report_input1.tab	kraken_mpa_report_input2.tab
+d__Bacteria	4	4
+d__Bacteria|p__Proteobacteria	4	4
+d__Bacteria|p__Proteobacteria|c__Gammaproteobacteria	4	4
+d__Bacteria|p__Proteobacteria|c__Gammaproteobacteria|o__Enterobacteriales	4	4
+d__Bacteria|p__Proteobacteria|c__Gammaproteobacteria|o__Enterobacteriales|f__Enterobacteriaceae	4	4
+d__Bacteria|p__Proteobacteria|c__Gammaproteobacteria|o__Enterobacteriales|f__Enterobacteriaceae|g__Escherichia	4	4
+d__Bacteria|p__Proteobacteria|c__Gammaproteobacteria|o__Enterobacteriales|f__Enterobacteriaceae|g__Escherichia|s__Escherichia_coli	4	4
diff --git a/tool_collections/kraken/kraken_report/test-data/kraken_report_test1.tab b/tool_collections/kraken/kraken_report/test-data/kraken_report_test1.tab
new file mode 100644
index 00000000000..2aa9e1ea8d5
--- /dev/null
+++ b/tool_collections/kraken/kraken_report/test-data/kraken_report_test1.tab
@@ -0,0 +1,4 @@
+C	gi|145231|gb|M33724.1|ECOALPHOA	83333	171	83333:162
+C	gi|145232|gb|M33725.1|ECOALPHOB	83333	183	83333:174
+C	gi|145234|gb|M33727.1|ECOALPHOE	83333	97	83333:88
+C	gi|146195|gb|J01619.1|ECOGLTA	83333	3850	83333:3841
diff --git a/tool_collections/kraken/kraken_report/test-data/kraken_report_test1_output.tab b/tool_collections/kraken/kraken_report/test-data/kraken_report_test1_output.tab
new file mode 100644
index 00000000000..1fcaa3e8cd7
--- /dev/null
+++ b/tool_collections/kraken/kraken_report/test-data/kraken_report_test1_output.tab
@@ -0,0 +1,11 @@
+  0.00	0	0	U	0	unclassified
+100.00	4	0	-	1	root
+100.00	4	0	-	131567	  cellular organisms
+100.00	4	0	D	2	    Bacteria
+100.00	4	0	P	1224	      Proteobacteria
+100.00	4	0	C	1236	        Gammaproteobacteria
+100.00	4	0	O	91347	          Enterobacteriales
+100.00	4	0	F	543	            Enterobacteriaceae
+100.00	4	0	G	561	              Escherichia
+100.00	4	0	S	562	                Escherichia coli
+100.00	4	4	-	83333	                  Escherichia coli K-12
diff --git a/tool_collections/kraken/kraken_report/test-data/test_database.loc b/tool_collections/kraken/kraken_report/test-data/test_database.loc
new file mode 120000
index 00000000000..03ec4336e45
--- /dev/null
+++ b/tool_collections/kraken/kraken_report/test-data/test_database.loc
@@ -0,0 +1 @@
+../../test_database.loc
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_report/test-data/test_db/database.idx b/tool_collections/kraken/kraken_report/test-data/test_db/database.idx
new file mode 120000
index 00000000000..9251273d383
--- /dev/null
+++ b/tool_collections/kraken/kraken_report/test-data/test_db/database.idx
@@ -0,0 +1 @@
+../../../database.idx
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_report/test-data/test_db/database.kdb b/tool_collections/kraken/kraken_report/test-data/test_db/database.kdb
new file mode 120000
index 00000000000..64efac5ce08
--- /dev/null
+++ b/tool_collections/kraken/kraken_report/test-data/test_db/database.kdb
@@ -0,0 +1 @@
+../../../database.kdb
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_report/test-data/test_db/taxonomy/names.dmp b/tool_collections/kraken/kraken_report/test-data/test_db/taxonomy/names.dmp
new file mode 120000
index 00000000000..f4585471fc2
--- /dev/null
+++ b/tool_collections/kraken/kraken_report/test-data/test_db/taxonomy/names.dmp
@@ -0,0 +1 @@
+../../../../names.dmp
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_report/test-data/test_db/taxonomy/nodes.dmp b/tool_collections/kraken/kraken_report/test-data/test_db/taxonomy/nodes.dmp
new file mode 120000
index 00000000000..a65d7d41f87
--- /dev/null
+++ b/tool_collections/kraken/kraken_report/test-data/test_db/taxonomy/nodes.dmp
@@ -0,0 +1 @@
+../../../../nodes.dmp
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_report/tool-data/kraken_databases.loc.sample b/tool_collections/kraken/kraken_report/tool-data/kraken_databases.loc.sample
new file mode 120000
index 00000000000..eba8983e9ea
--- /dev/null
+++ b/tool_collections/kraken/kraken_report/tool-data/kraken_databases.loc.sample
@@ -0,0 +1 @@
+../../kraken_databases.loc.sample
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_report/tool_data_table_conf.xml.sample b/tool_collections/kraken/kraken_report/tool_data_table_conf.xml.sample
new file mode 120000
index 00000000000..332baf440e9
--- /dev/null
+++ b/tool_collections/kraken/kraken_report/tool_data_table_conf.xml.sample
@@ -0,0 +1 @@
+../tool_data_table_conf.xml.sample
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_report/tool_data_table_conf.xml.test b/tool_collections/kraken/kraken_report/tool_data_table_conf.xml.test
new file mode 120000
index 00000000000..f8050b61701
--- /dev/null
+++ b/tool_collections/kraken/kraken_report/tool_data_table_conf.xml.test
@@ -0,0 +1 @@
+../tool_data_table_conf.xml.test
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_report/tool_dependencies.xml b/tool_collections/kraken/kraken_report/tool_dependencies.xml
new file mode 100644
index 00000000000..04f55a93faf
--- /dev/null
+++ b/tool_collections/kraken/kraken_report/tool_dependencies.xml
@@ -0,0 +1,6 @@
+<?xml version="1.0"?>
+<tool_dependency>
+  <package name="kraken" version="0.10.6-eaf8fb68">
+      <repository name="package_kraken_0_10_6_eaf8fb68" owner="iuc" />
+    </package>
+</tool_dependency>
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_translate/.shed.yml b/tool_collections/kraken/kraken_translate/.shed.yml
new file mode 100644
index 00000000000..37130f1d68e
--- /dev/null
+++ b/tool_collections/kraken/kraken_translate/.shed.yml
@@ -0,0 +1,16 @@
+homepage_url: http://ccb.jhu.edu/software/kraken/
+categories:
+- Metagenomics
+description: Kraken translate command.
+long_description: |
+  Kraken is a system for assigning taxonomic labels to short DNA
+  sequences, usually obtained through metagenomic studies. Previous attempts by other
+  bioinformatics software to accomplish this task have often used sequence alignment
+  or machine learning techniques that were quite slow, leading to the development
+  of less sensitive but much faster abundance estimation programs. Kraken aims to
+  achieve high sensitivity and high speed by utilizing exact alignments of k-mers
+  and a novel classification algorithm.
+name: kraken_translate
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/blob/master/tool_collections/kraken/kraken_translate/
+type: unrestricted
diff --git a/tool_collections/kraken/kraken_translate/README.rst b/tool_collections/kraken/kraken_translate/README.rst
new file mode 120000
index 00000000000..89a0106941f
--- /dev/null
+++ b/tool_collections/kraken/kraken_translate/README.rst
@@ -0,0 +1 @@
+../README.rst
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_translate/kraken-translate.xml b/tool_collections/kraken/kraken_translate/kraken-translate.xml
new file mode 100644
index 00000000000..e5a96b8db70
--- /dev/null
+++ b/tool_collections/kraken/kraken_translate/kraken-translate.xml
@@ -0,0 +1,60 @@
+<tool id="kraken-translate" name="Kraken-translate" version="1.2.1">
+    <description>
+        convert taxonomy IDs to names
+    </description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements" />
+    <expand macro="stdio" />
+    <expand macro="version_command" />
+    <command>
+        <![CDATA[
+        @SET_DATABASE_PATH@ &&
+        kraken-translate @INPUT_DATABASE@ $mpa_format "${input}" > "${translated}"
+        ]]>
+    </command>
+    <inputs>
+        <param format="tabular" label="Kraken output" name="input" type="data" help="Select taxonomy classification produced by kraken"/>
+        <param label="Restrict labels to standard rank assignments" name="mpa_format" truevalue="--mpa-format" falsevalue="" type="boolean" />
+        <expand macro="input_database" />
+    </inputs>
+    <outputs>
+        <data format="tabular" label="${tool.name} on ${on_string}: Translated classification" name="translated" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="input" value="kraken_translate_test1.tab" ftype="tabular"/>
+            <param name="mpa-format" value="false"/>
+            <param name="kraken_database" value="test_db"/>
+            <output name="translated" file="kraken_translate_test1_output.tab" ftype="tabular"/>
+        </test>
+    </tests>
+    <help>
+        <![CDATA[
+
+.. class:: warningmark
+
+**Note**: the database used must be the same as the one used in the original Kraken run
+
+-------
+
+**What it does**
+
+The file sequences.labels generated by the above example is a text file with two tab-delimited columns, and one line for each classified sequence in sequences.fa; unclassified sequences are not reported by kraken-translate. The first column of kraken-translate's output are the sequence IDs of the classified sequences, and the second column contains the taxonomy of the sequence. For example, an output line from kraken::
+
+ C     SEQ1    562     36      562:6
+
+Would result in a corresponding output line from kraken-translate::
+
+ SEQ1  root;cellular organisms;Bacteria;Proteobacteria;Gammaproteobacteria;Enterobacteriales;Enterobacteriaceae;Escherichia;Escherichia coli
+
+Alternatively, kraken-translate accepts the option ``--mpa-format`` which will report only levels of the taxonomy with standard rank assignments (superkingdom, kingdom, phylum, class, order, family, genus, species), and uses pipes to delimit the various levels of the taxonomy. For example, ``kraken-translate --mpa-format`` with the above example output from kraken would result in the following line of output::
+
+ SEQ1  d__Bacteria|p__Proteobacteria|c__Gammaproteobacteria|o__Enterobacteriales|f__Enterobacteriaceae|g__Escherichia|s__Escherichia_coli
+
+Taxonomy assignments above the superkingdom rank are represented as just "root" when using the ``--mpa-report`` option with kraken-translate.
+        ]]>
+    </help>
+    <expand macro="citations" />
+</tool>
diff --git a/tool_collections/kraken/kraken_translate/macros.xml b/tool_collections/kraken/kraken_translate/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/kraken/kraken_translate/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_translate/test-data/kraken_translate_test1.tab b/tool_collections/kraken/kraken_translate/test-data/kraken_translate_test1.tab
new file mode 100644
index 00000000000..2aa9e1ea8d5
--- /dev/null
+++ b/tool_collections/kraken/kraken_translate/test-data/kraken_translate_test1.tab
@@ -0,0 +1,4 @@
+C	gi|145231|gb|M33724.1|ECOALPHOA	83333	171	83333:162
+C	gi|145232|gb|M33725.1|ECOALPHOB	83333	183	83333:174
+C	gi|145234|gb|M33727.1|ECOALPHOE	83333	97	83333:88
+C	gi|146195|gb|J01619.1|ECOGLTA	83333	3850	83333:3841
diff --git a/tool_collections/kraken/kraken_translate/test-data/kraken_translate_test1_output.tab b/tool_collections/kraken/kraken_translate/test-data/kraken_translate_test1_output.tab
new file mode 100644
index 00000000000..e187cd5469c
--- /dev/null
+++ b/tool_collections/kraken/kraken_translate/test-data/kraken_translate_test1_output.tab
@@ -0,0 +1,4 @@
+gi|145231|gb|M33724.1|ECOALPHOA	root;cellular organisms;Bacteria;Proteobacteria;Gammaproteobacteria;Enterobacteriales;Enterobacteriaceae;Escherichia;Escherichia coli;Escherichia coli K-12
+gi|145232|gb|M33725.1|ECOALPHOB	root;cellular organisms;Bacteria;Proteobacteria;Gammaproteobacteria;Enterobacteriales;Enterobacteriaceae;Escherichia;Escherichia coli;Escherichia coli K-12
+gi|145234|gb|M33727.1|ECOALPHOE	root;cellular organisms;Bacteria;Proteobacteria;Gammaproteobacteria;Enterobacteriales;Enterobacteriaceae;Escherichia;Escherichia coli;Escherichia coli K-12
+gi|146195|gb|J01619.1|ECOGLTA	root;cellular organisms;Bacteria;Proteobacteria;Gammaproteobacteria;Enterobacteriales;Enterobacteriaceae;Escherichia;Escherichia coli;Escherichia coli K-12
diff --git a/tool_collections/kraken/kraken_translate/test-data/test_database.loc b/tool_collections/kraken/kraken_translate/test-data/test_database.loc
new file mode 120000
index 00000000000..03ec4336e45
--- /dev/null
+++ b/tool_collections/kraken/kraken_translate/test-data/test_database.loc
@@ -0,0 +1 @@
+../../test_database.loc
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_translate/test-data/test_db/database.idx b/tool_collections/kraken/kraken_translate/test-data/test_db/database.idx
new file mode 120000
index 00000000000..9251273d383
--- /dev/null
+++ b/tool_collections/kraken/kraken_translate/test-data/test_db/database.idx
@@ -0,0 +1 @@
+../../../database.idx
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_translate/test-data/test_db/database.kdb b/tool_collections/kraken/kraken_translate/test-data/test_db/database.kdb
new file mode 120000
index 00000000000..64efac5ce08
--- /dev/null
+++ b/tool_collections/kraken/kraken_translate/test-data/test_db/database.kdb
@@ -0,0 +1 @@
+../../../database.kdb
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_translate/test-data/test_db/taxonomy/names.dmp b/tool_collections/kraken/kraken_translate/test-data/test_db/taxonomy/names.dmp
new file mode 120000
index 00000000000..f4585471fc2
--- /dev/null
+++ b/tool_collections/kraken/kraken_translate/test-data/test_db/taxonomy/names.dmp
@@ -0,0 +1 @@
+../../../../names.dmp
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_translate/test-data/test_db/taxonomy/nodes.dmp b/tool_collections/kraken/kraken_translate/test-data/test_db/taxonomy/nodes.dmp
new file mode 120000
index 00000000000..a65d7d41f87
--- /dev/null
+++ b/tool_collections/kraken/kraken_translate/test-data/test_db/taxonomy/nodes.dmp
@@ -0,0 +1 @@
+../../../../nodes.dmp
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_translate/tool-data/kraken_databases.loc.sample b/tool_collections/kraken/kraken_translate/tool-data/kraken_databases.loc.sample
new file mode 120000
index 00000000000..eba8983e9ea
--- /dev/null
+++ b/tool_collections/kraken/kraken_translate/tool-data/kraken_databases.loc.sample
@@ -0,0 +1 @@
+../../kraken_databases.loc.sample
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_translate/tool_data_table_conf.xml.sample b/tool_collections/kraken/kraken_translate/tool_data_table_conf.xml.sample
new file mode 120000
index 00000000000..332baf440e9
--- /dev/null
+++ b/tool_collections/kraken/kraken_translate/tool_data_table_conf.xml.sample
@@ -0,0 +1 @@
+../tool_data_table_conf.xml.sample
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_translate/tool_data_table_conf.xml.test b/tool_collections/kraken/kraken_translate/tool_data_table_conf.xml.test
new file mode 120000
index 00000000000..f8050b61701
--- /dev/null
+++ b/tool_collections/kraken/kraken_translate/tool_data_table_conf.xml.test
@@ -0,0 +1 @@
+../tool_data_table_conf.xml.test
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_translate/tool_dependencies.xml b/tool_collections/kraken/kraken_translate/tool_dependencies.xml
new file mode 100644
index 00000000000..04f55a93faf
--- /dev/null
+++ b/tool_collections/kraken/kraken_translate/tool_dependencies.xml
@@ -0,0 +1,6 @@
+<?xml version="1.0"?>
+<tool_dependency>
+  <package name="kraken" version="0.10.6-eaf8fb68">
+      <repository name="package_kraken_0_10_6_eaf8fb68" owner="iuc" />
+    </package>
+</tool_dependency>
\ No newline at end of file
diff --git a/tool_collections/kraken/macros.xml b/tool_collections/kraken/macros.xml
new file mode 100644
index 00000000000..0943abbbe5e
--- /dev/null
+++ b/tool_collections/kraken/macros.xml
@@ -0,0 +1,31 @@
+<?xml version="1.0"?>
+<macros>
+    <xml name="requirements">
+        <requirements>
+            <requirement type="package" version="0.10.6_eaf8fb68">kraken</requirement>
+            <requirement type="package" version="0.10.6-eaf8fb68">kraken</requirement>
+        </requirements>
+    </xml>
+    <xml name="stdio">
+        <stdio>
+            <exit_code description="Tool exception" level="fatal" range="1:" />
+        </stdio>
+    </xml>
+    <xml name="version_command">
+        <version_command>export LC_ALL=C &amp;&amp; kraken --version</version_command>
+    </xml>
+    <xml name="input_database">
+        <param label="Select a Kraken database" name="kraken_database" type="select">
+            <options from_data_table="kraken_databases">
+                <validator message="No databases are available built-in" type="no_options" />
+            </options>
+        </param>
+    </xml>
+    <xml name="citations">
+        <citations>
+            <citation type="doi">10.1186/gb-2014-15-3-r46</citation>
+        </citations>
+    </xml>
+    <token name="@INPUT_DATABASE@">--db ${kraken_database.fields.name}</token>
+    <token name="@SET_DATABASE_PATH@">export KRAKEN_DB_PATH="${kraken_database.fields.path}"</token>
+</macros>
\ No newline at end of file
diff --git a/tool_collections/kraken/names.dmp b/tool_collections/kraken/names.dmp
new file mode 100644
index 00000000000..fa5e7ef33d4
--- /dev/null
+++ b/tool_collections/kraken/names.dmp
@@ -0,0 +1,74 @@
+83333	|	Escherichia coli K-12	|		|	scientific name	|
+83333	|	Escherichia coli K12	|		|	equivalent name	|
+562	|	"Bacillus coli" Migula 1895	|		|	authority	|
+562	|	"Bacterium coli commune" Escherich 1885	|		|	authority	|
+562	|	"Bacterium coli" (Migula 1895) Lehmann and Neumann 1896	|		|	authority	|
+562	|	ATCC 11775	|		|	type material	|
+562	|	Bacillus coli	|		|	synonym	|
+562	|	Bacterium coli	|		|	synonym	|
+562	|	Bacterium coli commune	|		|	synonym	|
+562	|	CCUG 24	|		|	type material	|
+562	|	CCUG 29300	|		|	type material	|
+562	|	CIP 54.8	|		|	type material	|
+562	|	DSM 30083	|		|	type material	|
+562	|	Enterococcus coli	|		|	synonym	|
+562	|	Escherchia coli	|		|	misspelling	|
+562	|	Escherichia coli	|		|	scientific name	|
+562	|	Escherichia coli (Migula 1895) Castellani and Chalmers 1919	|		|	authority	|
+562	|	Escherichia sp. MAR	|		|	includes	|
+562	|	Escherichia/Shigella coli	|		|	equivalent name	|
+562	|	Eschericia coli	|		|	misspelling	|
+562	|	JCM 1649	|		|	type material	|
+562	|	LMG 2092	|		|	type material	|
+562	|	NBRC 102203	|		|	type material	|
+562	|	NCCB 54008	|		|	type material	|
+562	|	NCTC 9001	|		|	type material	|
+562	|	bacterium 10a	|		|	includes	|
+562	|	bacterium E3	|		|	includes	|
+561	|	Escherchia	|		|	misspelling	|
+561	|	Escherichia	|		|	scientific name	|
+561	|	Escherichia Castellani and Chalmers 1919	|		|	authority	|
+543	|	Enterobacteraceae	|		|	synonym	|
+543	|	Enterobacteraceae (ex Lapage 1979) Lapage 1982, fam. nov., nom. rev.	|		|	synonym	|
+543	|	Enterobacteriaceae	|		|	scientific name	|
+543	|	Enterobacteriaceae (ex Rahn 1937) Ewing et al. 1980, fam. nov., nom. rev.	|		|	synonym	|
+543	|	Enterobacteriaceae Rahn 1937	|		|	synonym	|
+543	|	gamma-3 proteobacteria	|	gamma-3 proteobacteria <#1>	|	in-part	|
+91347	|	'Enterobacteriales'	|		|	synonym	|
+91347	|	Enterobacteriaceae and related endosymbionts	|		|	synonym	|
+91347	|	Enterobacteriaceae group	|		|	synonym	|
+91347	|	Enterobacteriales	|		|	scientific name	|
+91347	|	enterobacteria	|	enterobacteria<blast91347>	|	blast name	|
+91347	|	gamma-3 proteobacteria	|	gamma-3 proteobacteria <#5>	|	in-part	|
+1236	|	Gammaproteobacteria	|		|	scientific name	|
+1236	|	Gammaproteobacteria Garrity et al. 2005	|		|	synonym	|
+1236	|	Proteobacteria gamma subdivision	|		|	synonym	|
+1236	|	Purple bacteria, gamma subdivision	|		|	synonym	|
+1236	|	g-proteobacteria	|	gamma proteos<blast1236>	|	blast name	|
+1236	|	gamma proteobacteria	|		|	synonym	|
+1236	|	gamma subdivision	|		|	synonym	|
+1236	|	gamma subgroup	|		|	synonym	|
+1224	|	Proteobacteria	|		|	scientific name	|
+1224	|	Proteobacteria Garrity et al. 2005	|		|	authority	|
+1224	|	Proteobacteria [class] Stackebrandt et al. 1988	|		|	authority	|
+1224	|	not Proteobacteria Cavalier-Smith 2002	|		|	authority	|
+1224	|	proteobacteria	|	proteobacteria<blast1224>	|	blast name	|
+1224	|	purple bacteria	|		|	common name	|
+1224	|	purple bacteria and relatives	|		|	common name	|
+1224	|	purple non-sulfur bacteria	|		|	common name	|
+1224	|	purple photosynthetic bacteria	|		|	common name	|
+1224	|	purple photosynthetic bacteria and relatives	|		|	common name	|
+2	|	Bacteria	|	Bacteria <prokaryote>	|	scientific name	|
+2	|	Monera	|	Monera <Bacteria>	|	in-part	|
+2	|	Procaryotae	|	Procaryotae <Bacteria>	|	in-part	|
+2	|	Prokaryota	|	Prokaryota <Bacteria>	|	in-part	|
+2	|	Prokaryotae	|	Prokaryotae <Bacteria>	|	in-part	|
+2	|	bacteria	|	bacteria <blast2>	|	blast name	|
+2	|	eubacteria	|		|	genbank common name	|
+2	|	not Bacteria Haeckel 1894	|		|	synonym	|
+2	|	prokaryote	|	prokaryote <Bacteria>	|	in-part	|
+2	|	prokaryotes	|	prokaryotes <Bacteria>	|	in-part	|
+1	|	all	|		|	synonym	|
+1	|	root	|		|	scientific name	|
+131567	|	biota	|		|	synonym	|
+131567	|	cellular organisms	|		|	scientific name	|
diff --git a/tool_collections/kraken/nodes.dmp b/tool_collections/kraken/nodes.dmp
new file mode 100644
index 00000000000..4e292c27101
--- /dev/null
+++ b/tool_collections/kraken/nodes.dmp
@@ -0,0 +1,10 @@
+83333	|	562	|	no rank	|		|	0	|	1	|	11	|	1	|	0	|	1	|	1	|	0	|		|
+562	|	561	|	species	|	EC	|	0	|	1	|	11	|	1	|	0	|	1	|	1	|	0	|		|
+561	|	543	|	genus	|		|	0	|	1	|	11	|	1	|	0	|	1	|	0	|	0	|		|
+543	|	91347	|	family	|		|	0	|	1	|	11	|	1	|	0	|	1	|	0	|	0	|		|
+91347	|	1236	|	order	|		|	0	|	1	|	11	|	1	|	0	|	1	|	0	|	0	|		|
+1236	|	1224	|	class	|		|	0	|	1	|	11	|	1	|	0	|	1	|	0	|	0	|		|
+1224	|	2	|	phylum	|		|	0	|	1	|	11	|	1	|	0	|	1	|	0	|	0	|		|
+2	|	131567	|	superkingdom	|		|	0	|	0	|	11	|	0	|	0	|	0	|	0	|	0	|		|
+131567	|	1	|	no rank	|		|	8	|	1	|	1	|	1	|	0	|	1	|	1	|	0	|		|
+1	|	1	|	no rank	|		|	8	|	0	|	1	|	0	|	0	|	0	|	0	|	0	|		|
diff --git a/tool_collections/kraken/test_database.loc b/tool_collections/kraken/test_database.loc
new file mode 100644
index 00000000000..8441f152d69
--- /dev/null
+++ b/tool_collections/kraken/test_database.loc
@@ -0,0 +1 @@
+test_db	test_db	${__HERE__}
\ No newline at end of file
diff --git a/tool_collections/kraken/tool_data_table_conf.xml.sample b/tool_collections/kraken/tool_data_table_conf.xml.sample
new file mode 100644
index 00000000000..c7763ac28af
--- /dev/null
+++ b/tool_collections/kraken/tool_data_table_conf.xml.sample
@@ -0,0 +1,8 @@
+<?xml version="1.0"?>
+<tables>
+    <!-- Locations of Kraken database in the required format -->
+    <table name="kraken_databases" comment_char="#">
+        <columns>value, name, path</columns>
+        <file path="tool-data/kraken_databases.loc" />
+    </table>
+</tables>
diff --git a/tool_collections/kraken/tool_data_table_conf.xml.test b/tool_collections/kraken/tool_data_table_conf.xml.test
new file mode 100644
index 00000000000..690ae6b4748
--- /dev/null
+++ b/tool_collections/kraken/tool_data_table_conf.xml.test
@@ -0,0 +1,8 @@
+<?xml version="1.0"?>
+<tables>
+    <!-- Locations of Kraken database in the required format -->
+    <table name="kraken_databases" comment_char="#">
+        <columns>value, name, path</columns>
+        <file path="${__HERE__}/test-data/test_database.loc" />
+    </table>
+</tables>
diff --git a/tool_collections/samtools/bam_to_cram/.shed.yml b/tool_collections/samtools/bam_to_cram/.shed.yml
new file mode 100644
index 00000000000..ebd687fbc8e
--- /dev/null
+++ b/tool_collections/samtools/bam_to_cram/.shed.yml
@@ -0,0 +1,13 @@
+categories:
+- Convert Formats
+- SAM
+description: Converts alignments in BAM format to CRAM format.
+long_description: |
+  This tool uses the SAMtools toolkit to convert BAM to CRAM files. The CRAM
+  format does additional compression relative to the reference genome which
+  makes the compression more effictient in terms of file size.
+name: samtools_bam_to_cram
+owner: devteam
+homepage_url: https://samtools.github.io/
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/bam_to_cram
+type: unrestricted
diff --git a/tool_collections/samtools/bam_to_cram/macros.xml b/tool_collections/samtools/bam_to_cram/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/samtools/bam_to_cram/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/bam_to_cram/samtools_bam_to_cram.xml b/tool_collections/samtools/bam_to_cram/samtools_bam_to_cram.xml
new file mode 100644
index 00000000000..87838f8347c
--- /dev/null
+++ b/tool_collections/samtools/bam_to_cram/samtools_bam_to_cram.xml
@@ -0,0 +1,125 @@
+<tool id="samtools_bam_to_cram" name="samtools BAM to CRAM" version="1.2.0">
+    <description>convert BAM alignments to CRAM format</description>
+
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"></expand>
+    <expand macro="version_command"></expand>
+    <expand macro="stdio"></expand>
+
+    <command>
+    <![CDATA[
+        #if str( $input_alignment.metadata.bam_index ) != "None":
+            ln
+                -f
+                -s
+                "${input_alignment.metadata.bam_index}"
+                "${input_alignment}.bai"
+                &&
+        #end if
+
+        samtools view
+            #if $parameter_regions.target_region == "regions_bed_file"
+                -L "${parameter_regions.regions_bed_file}"
+            #end if
+            
+            -@\${GALAXY_SLOTS:-2}
+            -C
+            -T "${reference_source.input_reference}"
+            -o "$output_alignment"
+            "$input_alignment"
+            
+            #if $parameter_regions.target_region == "region"
+                "${parameter_regions.region_string}"
+            #end if
+    ]]>
+    </command>
+
+    <inputs>
+        <param name="input_alignment" type="data" format="bam,sam" label="BAM (or SAM) alignment file"/>
+        <conditional name="reference_source">
+            <param name="reference_source_selector" type="select" label="Load reference genome from">
+                <option value="cached">Local cache</option>
+                <option value="history">History</option>
+            </param>
+            <when value="cached">
+                <param name="input_reference" type="select" format="fasta" label="Genome reference FASTA file">
+                    <options from_data_table="all_fasta">
+                        <filter type="data_meta" ref="input_alignment" key="dbkey" column="1" />
+                    </options>
+                    <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/>
+                </param>
+            </when>
+            <when value="history">
+                <param name="input_reference" type="data" format="fasta" label="Genome reference FASTA file"/>
+            </when>
+        </conditional>
+        <conditional name="parameter_regions">
+            <param name="target_region" type="select" label="Choose conversion within specific genomic region(s)">
+                <option value="entire_input_file">Entire BAM alignment file</option>
+                <option value="region">Specific region</option>
+                <option value="regions_bed_file">List of specific regions (BED file)</option>
+            </param>
+            <when value="entire_input_file" />
+            <when value="region">
+                <param name="region_string" type="text" label="Samtools: region in which pileup is generated" help="e.g. chrX or chr:start-end" />
+            </when>
+            <when value="regions_bed_file">
+                <param name="regions_bed_file" type="data" format="bed" label="only include reads overlapping this BED FILE" help="-L"/>
+            </when>
+        </conditional>
+    </inputs>
+
+    <outputs>
+        <data format="cram" name="output_alignment" label="$tool.name on ${on_string}.cram"></data>
+    </outputs>
+
+    <tests>
+        <test>
+            <param name="input_alignment" value="test.bam" ftype="bam" />
+            <param name="reference_source_selector" value="history" />
+            <param name="input_reference" value="test.fa" />
+            <param name="target_region" value="entire_input_file" />
+
+            <output name="output_alignment" file="test.cram"  compare="sim_size" delta="250" />
+        </test>
+        <test>
+            <param name="input_alignment" value="test.sam" ftype="sam" />
+            <param name="reference_source_selector" value="history" />
+            <param name="input_reference" value="test.fa" />
+            <param name="target_region" value="entire_input_file" />
+
+            <output name="output_alignment" file="test.cram"  compare="sim_size" delta="250" />
+        </test>
+        <test>
+            <param name="input_alignment" value="test.bam" ftype="bam" />
+            <param name="reference_source_selector" value="history" />
+            <param name="input_reference" value="test.fa" />
+            <param name="target_region" value="region" />
+            <param name="region_string" value="CHROMOSOME_I" />
+
+            <output name="output_alignment" file="test.cram"  compare="sim_size" delta="250" />
+        </test>
+        <test>
+            <param name="input_alignment" value="test.bam" ftype="bam" />
+            <param name="reference_source_selector" value="history" />
+            <param name="input_reference" value="test.fa" />
+            <param name="target_region" value="regions_bed_file" />
+            <param name="regions_bed_file" value="test.bed" ftype="bed" />
+
+            <output name="output_alignment" file="test.cram"  compare="sim_size" delta="250" />
+        </test>
+    </tests>
+
+    <help>
+
+**What this tool does**
+
+Converts alignments from the BAM format to the CRAM format using the SAMTools_ toolkit. The CRAM format does additional compression relative to the reference genome which makes the compression in terms of file size more effictient.
+
+.. _SAMTools: http://samtools.sourceforge.net/samtools.shtml
+    </help>
+    
+    <expand macro="citations"></expand>
+</tool>
diff --git a/tool_collections/samtools/bam_to_cram/test-data/test.bam b/tool_collections/samtools/bam_to_cram/test-data/test.bam
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diff --git a/tool_collections/samtools/bam_to_cram/test-data/test.bed b/tool_collections/samtools/bam_to_cram/test-data/test.bed
new file mode 100644
index 00000000000..43dabf1de84
--- /dev/null
+++ b/tool_collections/samtools/bam_to_cram/test-data/test.bed
@@ -0,0 +1 @@
+CHROMOSOME_I	1	120
diff --git a/tool_collections/samtools/bam_to_cram/test-data/test.cram b/tool_collections/samtools/bam_to_cram/test-data/test.cram
new file mode 100644
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z1Q3D$Gl5<Lt{!K_Sks-yKtm@6u!gpVwig6Nd7JEd%5I|UX3B1%G&F0c*_Vp8VUb?S
zPE(whHpO#dGXsqk)L&hz)e%uR7K;Nw=?DQJ0A@47(Frh(m5TrvvFV`)HZ*Y=a5U3|
z2t0(c3hLI7uZUh8jo~VtnGg$bkw7Q_I6>$rLPTiu>;%AZot0QC22rep5D4`~hZqo0
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zhcN8HzIWHe!%QFm&t->;FoJcucJ7_o4-doSwOvu$>$y>GWU$%quKbzJr<3s;meVEC
zuAnp-C$KyH?A8r@T(Z5sZ#kj($HC~c3Xx#2D6S?U*cJWN!yWfpruX!X9{T2s_uTSN
zKfJ#1QO7uQ5sYYY>QrNmYLYw=ZTPI{Y1`C#`D$D1{mLyR@-NjB9jgxgl=*fa-$B{{
zn&!hT1jLcS$;!*txHk*Gx8&nP#`aq`dx!gH<_5ZG*#{?W1tsTq<UCW^RlaesxhLV=
zwPU||O~vksK(>2kVz;B?W8c2i^0%HmK+Xj&pULSR9__w4b-L-<+quO@dYVaZ%J1u{
z`sYV7&y@ewSv~&8?UCV6jvh30{;D(-OuNpf44w^^dX0x;I;Ugf=ZpW&Ib5|_+^Pp`
zz4wA~r*^3A=+C>W_Fg$YTla&x@rGh_<iyvk-1C0m^XKhPookDEoLs+d&Dh<<yDbkK
zv;DJA1ndjZ%DHdpAzf4E!tq~xCHD@zq0egG)^j1YBx~%()vST!f%_6g``GDuczY#~
W1%C{J`4%12;Z1_Ss3`7A0qh@NJ=y*M

literal 0
HcmV?d00001

diff --git a/tool_collections/samtools/bam_to_cram/test-data/test.fa b/tool_collections/samtools/bam_to_cram/test-data/test.fa
new file mode 100644
index 00000000000..da7f291fc38
--- /dev/null
+++ b/tool_collections/samtools/bam_to_cram/test-data/test.fa
@@ -0,0 +1,3 @@
+>CHROMOSOME_I
+gcctaagcctaagcctaagcctaagcctaagcctaagcctaagcctaagc
+ctaagcctaagcctaagcctaagcctaagcctaagcctaagcctaagcct
diff --git a/tool_collections/samtools/bam_to_cram/test-data/test.sam b/tool_collections/samtools/bam_to_cram/test-data/test.sam
new file mode 100644
index 00000000000..055905b377e
--- /dev/null
+++ b/tool_collections/samtools/bam_to_cram/test-data/test.sam
@@ -0,0 +1,15 @@
+@HD	VN:1.4	SO:unsorted
+@SQ	SN:CHROMOSOME_I	LN:100
+@RG	ID:UNKNOWN	SM:UNKNOWN
+@PG	ID:bowtie2	PN:bowtie2	VN:2.0.0-beta5
+@PG	ID:0	CL:aaaaa/aaa/aaaaa/aaaaaa/aaaaaaaaa/aaa/iuc/package_aaaaaaaaa_x_y/aaaaaaaaaaaa/bin/aaaaaaaaaaaaaaaaa aaaaaaaaaa /aaaa/aaaaa/aaa/aaaaaaaaaaaaaaaaaaa/tools/aaaaaaaaa/test-data/test.cram aa /aaaa/aaaaa/aaa/aaaaaaaaaaaaaaaaaaa/tools/aaaaaaaaa/test-data/test.fa -O test	PN:samtools	VN:1.2
+SRR065390.14978392	16	CHROMOSOME_I	2	1	27M1D73M	*	0	0	CCTAGCCCTAACCCTAACCCTAACCCTAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAA	#############################@B?8B?BA@@DDBCDDCBC@CDCDCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC	RG:Z:UNKNOWN	XG:i:1	XM:i:5	XN:i:0	XO:i:1	AS:i:-18	XS:i:-18	YT:Z:UU
+SRR065390.921023	16	CHROMOSOME_I	3	12	100M	*	0	0	CTAAGCCTAAATCTAAGCCTAACCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAA	###############################################???88:;98768700000<>:BBA?BBAB?BBBBBBBB>B>BB::;?:00000	RG:Z:UNKNOWN	XG:i:0	XM:i:3	XN:i:0	XO:i:0	AS:i:-6	XS:i:-13	YT:Z:UU
+SRR065390.1871511	16	CHROMOSOME_I	3	1	100M	*	0	0	CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAA	<?@<@A8>0:BB@>B<=B@???@=8@B>BB@CA@DACDCBBCCCA@CCCCACCBCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC	RG:Z:UNKNOWN	XG:i:0	XM:i:0	XN:i:0	XO:i:0	AS:i:0	XS:i:0	YT:Z:UU
+SRR065390.3743423	16	CHROMOSOME_I	3	1	100M	*	0	0	CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAA	##################?6@:7<=@3=@ABAAB>BDBBABADABDDDBDDBCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC	RG:Z:UNKNOWN	XG:i:0	XM:i:0	XN:i:0	XO:i:0	AS:i:0	XS:i:0	YT:Z:UU
+SRR065390.4251890	16	CHROMOSOME_I	3	1	100M	*	0	0	CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAA	###########@BB=BCBBC?B>B;>B@@ADBBB@DBBBBDCCBBCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC	RG:Z:UNKNOWN	XG:i:0	XM:i:0	XN:i:0	XO:i:0	AS:i:0	XS:i:0	YT:Z:UU
+SRR065390.5238868	16	CHROMOSOME_I	3	1	100M	*	0	0	CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAA	@,=@@D8D;?BBB>;?BBB==BB@D;>D>BBB>BBDDB<DABADCACDCCBCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC	RG:Z:UNKNOWN	XG:i:0	XM:i:0	XN:i:0	XO:i:0	AS:i:0	XS:i:0	YT:Z:UU
+SRR065390.6023338	0	CHROMOSOME_I	3	1	100M	*	0	0	CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAAGCTAC	CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC@CCDDDBCCABB=DABBA?################	RG:Z:UNKNOWN	XG:i:0	XM:i:3	XN:i:0	XO:i:0	AS:i:-6	XS:i:-6	YT:Z:UU
+SRR065390.6815812	16	CHROMOSOME_I	3	1	100M	*	0	0	CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAA	###############?@.@?B;B0B=;<DADB@@BDDBBDDBCBCBD@CCDCCCCCCCDCCCCCCCCACCCCCCCCCCBCCCCCCDCCCCCCCCCCCBCC	RG:Z:UNKNOWN	XG:i:0	XM:i:0	XN:i:0	XO:i:0	AS:i:0	XS:i:0	YT:Z:UU
+SRR065390.6905811	16	CHROMOSOME_I	3	1	100M	*	0	0	CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAA	#######################BB@>A<BC>@@BCCB@=BACBCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC	RG:Z:UNKNOWN	XG:i:0	XM:i:0	XN:i:0	XO:i:0	AS:i:0	XS:i:0	YT:Z:UU
+SRR065390.8289592	16	CHROMOSOME_I	3	1	100M	*	0	0	CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAA	###############################A?@C9@@BC=AABDD@A@DC@CB=@BA?6@CCAAC@+CCCCCCCCCCCCCCC@CCCCCCCCCCCCCCCC	RG:Z:UNKNOWN	XG:i:0	XM:i:0	XN:i:0	XO:i:0	AS:i:0	XS:i:0	YT:Z:UU
diff --git a/tool_collections/samtools/bam_to_cram/tool_dependencies.xml b/tool_collections/samtools/bam_to_cram/tool_dependencies.xml
new file mode 120000
index 00000000000..b11b378d63c
--- /dev/null
+++ b/tool_collections/samtools/bam_to_cram/tool_dependencies.xml
@@ -0,0 +1 @@
+../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/bam_to_sam/.shed.yml b/tool_collections/samtools/bam_to_sam/.shed.yml
new file mode 100644
index 00000000000..cf0ac3af3a0
--- /dev/null
+++ b/tool_collections/samtools/bam_to_sam/.shed.yml
@@ -0,0 +1,11 @@
+categories:
+- SAM
+- Convert Formats
+description: Converts BAM format to SAM format.
+long_description: |
+  This tool uses the SAMtools toolkit to produce a SAM file from a BAM file.
+name: bam_to_sam
+owner: devteam
+homepage_url: http://www.htslib.org/
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/bam_to_sam
+type: unrestricted
diff --git a/tool_collections/samtools/bam_to_sam/bam_to_sam.xml b/tool_collections/samtools/bam_to_sam/bam_to_sam.xml
new file mode 100644
index 00000000000..faa8c97dbc1
--- /dev/null
+++ b/tool_collections/samtools/bam_to_sam/bam_to_sam.xml
@@ -0,0 +1,54 @@
+<tool id="bam_to_sam" name="BAM-to-SAM" version="2.0">
+  <macros>
+    <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"></expand>
+  <expand macro="version_command"></expand>
+  <expand macro="stdio"></expand>
+  <description>convert BAM to SAM</description>  
+    <command>
+<![CDATA[
+  samtools view -o "${output1}" ${header} "${input1}"
+]]>
+    </command>
+    <inputs>
+        <param format="bam" label="BAM File to Convert" name="input1" type="data" />
+        <param name="header" label="Header options" type="select" help="Allows to choose between seeing the entire dataset with the header, header only, or data only.">
+          <option value="-h">Include header in SAM output (-h)</option>
+          <option value="-H">Print header only (-H)</option>
+          <option value="">Exclude header</option>
+        </param>
+    </inputs>
+    <outputs>
+        <data format="sam" label="${tool.name} on ${on_string}: converted SAM" name="output1" />
+    </outputs>
+    <tests>
+        <test>
+            <param ftype="bam" name="input1" value="bam_to_sam_in1.bam" />
+            <param name="header" value="-h" />
+            <output file="bam_to_sam_out1.sam" name="output1" sorted="True" />
+        </test>
+        <test>
+            <param ftype="bam" name="input1" value="bam_to_sam_in1.bam" />
+            <param name="header" value="-H" />
+            <output file="bam_to_sam_out2.sam" name="output1" sorted="True" />
+        </test>
+        <test>
+            <param ftype="bam" name="input1" value="bam_to_sam_in1.bam" />
+            <param name="header" value="" />
+            <output file="bam_to_sam_out3.sam" name="output1" sorted="True" />
+        </test>
+    </tests>
+    <help>
+<![CDATA[
+
+**What it does**
+
+Converts BAM dataset to SAM using ``samtools view`` command::
+
+ samtools view -o [OUTPUT SAM] [-h|-H] [INPUT BAM] 
+
+]]>
+  </help>
+  <expand macro="citations"></expand>
+</tool>
diff --git a/tool_collections/samtools/bam_to_sam/macros.xml b/tool_collections/samtools/bam_to_sam/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/samtools/bam_to_sam/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/bam_to_sam/test-data/bam_to_sam_in1.bam b/tool_collections/samtools/bam_to_sam/test-data/bam_to_sam_in1.bam
new file mode 100644
index 0000000000000000000000000000000000000000..307e0c77d584b9bb59eae87f39f9c76beb3ea09f
GIT binary patch
literal 671
zcmV;Q0$}|giwFb&00000{{{d;LjnMT0@akyYuhjs$5l27ONkX*k!da)B)E{n)+M3P
zl2d8|bz%J(7;G2MOT0ELjf0a*E`yjJeBb_n!r1?@>-GoixWNuPuVshbvM0AoQ)pqw
z_G>>$@5#3Gv83m$Z!Nq<2sPX$eYRb%E>~!4r``_3sMGEHqkuJ9d$hG(Zy!cg`V=0h
zROdpgDzs8rnG3C{P_0s9jr|R}*{s9&w(4&8(N=g=WR2Z4R;9b!bvGCn)sVI23cCk0
zz+h?=6kIy1=>5XXSwml-@eK@OsUA!>#R6)jZ>ZQ~C_Y2|DjXepQQ&ump5N;p^u2bk
zJMfPD=uOZ;y9A-4kf!r{r%-m?1a5mhiObHhIo(PA*wc6lDwOO(@+2uavV2;KOXe?)
z=u~HBM$<4Rm~t(PX;#BJ7+e{i#aP!ho$DDWDaIMBV+@@b=fFD`7bef-jJ%bDw#8|V
za+8~jf^%UlT8kX-Z@j3WpL2vuDdF>!aC#whm2eOR!=OL%!hUb;z3xWC(K+<+_LF{{
zBeiZ=O9koGyipj!0`|w=fj_tdWZy3+a$pZrvUf8w33dY0yMCAY9e*$iqPv4AXNc_?
zqQZW8<3YSLi2rhp+KcD8=EDq8Y4yfcoXa(5PO})F0ap^+MQ0+)C0odX?MT~>U01r3
zq|lrc<HWU(ZRd2|DF_2|L+10+0!f3XwI!usCO7gNa|tp`CV-p(ZI)1`X_OE{Frg97
z86$)fP2+-LDjCx-F&So@V)!*w!&#jh#JG}c6rnnS_!`1%Pv8l33bm2J_P{>fzvZMq
z&}9Z)O4&YMAyxXXta_^{{{o&auI$eT001A02m}BC000301^_}s0stET0{{R300000
F003)%IVb=C

literal 0
HcmV?d00001

diff --git a/tool_collections/samtools/bam_to_sam/test-data/bam_to_sam_in1.sam b/tool_collections/samtools/bam_to_sam/test-data/bam_to_sam_in1.sam
new file mode 100644
index 00000000000..4a1a6a7d5a6
--- /dev/null
+++ b/tool_collections/samtools/bam_to_sam/test-data/bam_to_sam_in1.sam
@@ -0,0 +1,14 @@
+@HD	VN:1.0	SO:coordinate
+@SQ	SN:chr1	LN:101
+@SQ	SN:chr7	LN:404
+@SQ	SN:chr8	LN:202
+@RG	ID:0	SM:Hi,Mom!
+@PG	ID:1	PN:Hey!	VN:2.0
+both_reads_align_clip_marked	83	chr7	1	255	101M	=	302	201	CAACAGAAGCNGGNATCTGTGTTTGTGTTTCGGATTTCCTGCTGAANNGNTTNTCGNNTCNNNNNNNNATCCCGATTTCNTTCCGCAGCTNACCTCCCAAN	)'.*.+2,))&&'&*/)-&*-)&.-)&)&),/-&&..)./.,.).*&&,&.&&-)&&&0*&&&&&&&&/32/,01460&&/6/*0*/2/283//36868/&	RG:Z:0
+both_reads_present_only_first_aligns	89	chr7	1	255	101M	*	0	0	CAACAGAAGCNGGNATCTGTGTTTGTGTTTCGGATTTCCTGCTGAANNGNTTNTCGNNTCNNNNNNNNATCCCGATTTCNTTCCGCAGCTNACCTCCCAAN	)'.*.+2,))&&'&*/)-&*-)&.-)&)&),/-&&..)./.,.).*&&,&.&&-)&&&0*&&&&&&&&/32/,01460&&/6/*0*/2/283//36868/&	RG:Z:0
+read_2_too_many_gaps	83	chr7	1	255	101M	=	302	201	CAACAGAAGCNGGNATCTGTGTTTGTGTTTCGGATTTCCTGCTGAANNGNTTNTCGNNTCNNNNNNNNATCCCGATTTCNTTCCGCAGCTNACCTCCCAAN	)'.*.+2,))&&'&*/)-&*-)&.-)&)&),/-&&..)./.,.).*&&,&.&&-)&&&0*&&&&&&&&/32/,01460&&/6/*0*/2/283//36868/&	RG:Z:0
+both_reads_align_clip_adapter	147	chr7	16	255	101M	=	21	-96	CAACAGAAGCNGGNATCTGTGTTTGTGTTTCGGATTTCCTGCTGAANNGNTTNTCGNNTCNNNNNNNNATCCCGATTTCNTTCCGCAGCTNACCTCCCAAN	)'.*.+2,))&&'&*/)-&*-)&.-)&)&),/-&&..)./.,.).*&&,&.&&-)&&&0*&&&&&&&&/32/,01460&&/6/*0*/2/283//36868/&	RG:Z:0
+both_reads_align_clip_adapter	99	chr7	21	255	101M	=	16	96	CAACAGAAGCNGGNATCTGTGTTTGTGTTTCGGATTTCCTGCTGAANNGNTTNTCGNNTCNNNNNNNNATCCCGATTTCNTTCCGCAGCTNACCTCCCAAN	)'.*.+2,))&&'&*/)-&*-)&.-)&)&),/-&&..)./.,.).*&&,&.&&-)&&&0*&&&&&&&&/32/,01460&&/6/*0*/2/283//36868/&	RG:Z:0
+both_reads_align_clip_marked	163	chr7	302	255	101M	=	1	-201	NCGCGGCATCNCGATTTCTTTCCGCAGCTAACCTCCCGACAGATCGGCAGCGCGTCGTGTAGGTTATTATGGTACATCTTGTCGTGCGGCNAGAGCATACA	&/15445666651/566666553+2/14/&/555512+3/)-'/-&-'*+))*''13+3)'//++''/'))/3+&*5++)&'2+&+/*&-&&*)&-./1'1	RG:Z:0
+read_2_too_many_gaps	163	chr7	302	255	10M1D10M5I76M	=	1	-201	NCGCGGCATCNCGATTTCTTTCCGCAGCTAACCTCCCGACAGATCGGCAGCGCGTCGTGTAGGTTATTATGGTACATCTTGTCGTGCGGCNAGAGCATACA	&/15445666651/566666553+2/14/&/555512+3/)-'/-&-'*+))*''13+3)'//++''/'))/3+&*5++)&'2+&+/*&-&&*)&-./1'1	RG:Z:0
+both_reads_present_only_first_aligns	165	*	0	0	*	chr7	1	0	NCGCGGCATCNCGATTTCTTTCCGCAGCTAACCTCCCGACAGATCGGCAGCGCGTCGTGTAGGTTATTATGGTACATCTTGTCGTGCGGCNAGAGCATACA	&/15445666651/566666553+2/14/&/555512+3/)-'/-&-'*+))*''13+3)'//++''/'))/3+&*5++)&'2+&+/*&-&&*)&-./1'1	RG:Z:0
diff --git a/tool_collections/samtools/bam_to_sam/test-data/bam_to_sam_in2.bam b/tool_collections/samtools/bam_to_sam/test-data/bam_to_sam_in2.bam
new file mode 100644
index 0000000000000000000000000000000000000000..258f13214811cf6de3949b27ae1053236d248752
GIT binary patch
literal 351
zcmb2|=3rp}f&Xj_PR>jWPK;A0o%K5GAmWzaqqj^!B9{A-Z~3H|p8t>ePIZbl5S1=?
zxbE1ZJe?l9X6uiVHGAw@{Vth#9z9pHSKv^Mq~E!{cUGUi@mp!py$$Ez9qXFkuCp`n
zWz9PI__*coH%IC3Og$N~^!%*N*~N-H&T*Vat?qa{tq)%unz89*L+>vy`@(5QUab}C
zu$i#6c5z9<yf@oLOuCvEyywraop4oOP`750;?z=)#mS3q?CGovoBEVLc<mP*Q`uD=
zO?&#xvW#|^XYJk;bvtrVf?>~5LH!A;g6k(Yea-y7>UpVH$m{;_lbfox3a$_Cx#}3f
zyXH8@%Z(|KduMfsAKw#od+#)z{s(gf*8M#d@$}8wxI<63x__wiy#A&<p;SO@hp?~s
r>Er45OV8drUY(;HCoZu%JNYk@l;yq8>TC=Q@@PRQ&A<!}Q4j$D0g|3;

literal 0
HcmV?d00001

diff --git a/tool_collections/samtools/bam_to_sam/test-data/bam_to_sam_in2.sam b/tool_collections/samtools/bam_to_sam/test-data/bam_to_sam_in2.sam
new file mode 100644
index 00000000000..b16e2455824
--- /dev/null
+++ b/tool_collections/samtools/bam_to_sam/test-data/bam_to_sam_in2.sam
@@ -0,0 +1,25 @@
+@HD	VN:1.0	SO:coordinate
+@SQ	SN:chr1	LN:10001
+@SQ	SN:chr2	LN:100001
+@SQ	SN:chr3	LN:10001
+@SQ	SN:chr4	LN:1001
+@RG	ID:rg1	SM:s1
+@RG	ID:rg2	SM:s3
+bar:record:4	77	chr1	1	0	*	*	0	0	AAAAAAAAAAAAA	1111111111111	RG:Z:rg1
+bar:record:6	77	chr1	1	0	*	*	0	0	AAAAAAAAAAAAA	1111111111111	RG:Z:rg2
+bar:record:1	77	chr1	10	0	*	*	0	0	AAAAAAAAAAAAA	1111111111111	RG:Z:rg1
+bar:record:3	77	chr1	10	0	*	*	0	0	AAAAAAAAAAAAA	1111111111111	RG:Z:rg2
+bar:record:1	141	chr1	20	0	*	*	0	0	CCCCCCCCCCCCC	2222222222222	RG:Z:rg1
+bar:record:7	77	chr1	20	0	*	*	0	0	AAAAAAAAAAAAA	1111111111111	RG:Z:rg2
+bar:record:8	77	chr1	30	0	*	*	0	0	AAAAAAAAAAAAA	1111111111111	RG:Z:rg2
+bar:record:4	141	chr1	40	0	*	*	0	0	CCCCCCCCCCCCC	2222222222222	RG:Z:rg1
+bar:record:5	77	chr1	40	0	*	*	0	0	AAAAAAAAAAAAA	1111111111111	RG:Z:rg2
+bar:record:6	141	chr1	50	0	*	*	0	0	CCCCCCCCCCCCC	2222222222222	RG:Z:rg2
+bar:record:2	77	chr2	10	0	*	*	0	0	AAAAAAAAAAAAA	1111111111111	RG:Z:rg1
+bar:record:2	141	chr2	30	0	*	*	0	0	CCCCCCCCCCCCC	2222222222222	RG:Z:rg2
+bar:record:3	141	chr3	20	0	*	*	0	0	CCCCCCCCCCCCC	2222222222222	RG:Z:rg1
+bar:record:8	141	chr3	20	0	*	*	0	0	CCCCCCCCCCCCC	2222222222222	RG:Z:rg1
+bar:record:5	141	chr3	40	0	*	*	0	0	CCCCCCCCCCCCC	2222222222222	RG:Z:rg1
+bar:record:9	77	chr4	10	0	*	*	0	0	AAAAAAAAAAAAA	1111111111111	RG:Z:rg1
+bar:record:7	141	chr4	20	0	*	*	0	0	CCCCCCCCCCCCC	2222222222222	RG:Z:rg1
+bar:record:9	141	chr4	60	0	*	*	0	0	CCCCCCCCCCCCC	2222222222222	RG:Z:rg1
diff --git a/tool_collections/samtools/bam_to_sam/test-data/bam_to_sam_out1.sam b/tool_collections/samtools/bam_to_sam/test-data/bam_to_sam_out1.sam
new file mode 100644
index 00000000000..4a1a6a7d5a6
--- /dev/null
+++ b/tool_collections/samtools/bam_to_sam/test-data/bam_to_sam_out1.sam
@@ -0,0 +1,14 @@
+@HD	VN:1.0	SO:coordinate
+@SQ	SN:chr1	LN:101
+@SQ	SN:chr7	LN:404
+@SQ	SN:chr8	LN:202
+@RG	ID:0	SM:Hi,Mom!
+@PG	ID:1	PN:Hey!	VN:2.0
+both_reads_align_clip_marked	83	chr7	1	255	101M	=	302	201	CAACAGAAGCNGGNATCTGTGTTTGTGTTTCGGATTTCCTGCTGAANNGNTTNTCGNNTCNNNNNNNNATCCCGATTTCNTTCCGCAGCTNACCTCCCAAN	)'.*.+2,))&&'&*/)-&*-)&.-)&)&),/-&&..)./.,.).*&&,&.&&-)&&&0*&&&&&&&&/32/,01460&&/6/*0*/2/283//36868/&	RG:Z:0
+both_reads_present_only_first_aligns	89	chr7	1	255	101M	*	0	0	CAACAGAAGCNGGNATCTGTGTTTGTGTTTCGGATTTCCTGCTGAANNGNTTNTCGNNTCNNNNNNNNATCCCGATTTCNTTCCGCAGCTNACCTCCCAAN	)'.*.+2,))&&'&*/)-&*-)&.-)&)&),/-&&..)./.,.).*&&,&.&&-)&&&0*&&&&&&&&/32/,01460&&/6/*0*/2/283//36868/&	RG:Z:0
+read_2_too_many_gaps	83	chr7	1	255	101M	=	302	201	CAACAGAAGCNGGNATCTGTGTTTGTGTTTCGGATTTCCTGCTGAANNGNTTNTCGNNTCNNNNNNNNATCCCGATTTCNTTCCGCAGCTNACCTCCCAAN	)'.*.+2,))&&'&*/)-&*-)&.-)&)&),/-&&..)./.,.).*&&,&.&&-)&&&0*&&&&&&&&/32/,01460&&/6/*0*/2/283//36868/&	RG:Z:0
+both_reads_align_clip_adapter	147	chr7	16	255	101M	=	21	-96	CAACAGAAGCNGGNATCTGTGTTTGTGTTTCGGATTTCCTGCTGAANNGNTTNTCGNNTCNNNNNNNNATCCCGATTTCNTTCCGCAGCTNACCTCCCAAN	)'.*.+2,))&&'&*/)-&*-)&.-)&)&),/-&&..)./.,.).*&&,&.&&-)&&&0*&&&&&&&&/32/,01460&&/6/*0*/2/283//36868/&	RG:Z:0
+both_reads_align_clip_adapter	99	chr7	21	255	101M	=	16	96	CAACAGAAGCNGGNATCTGTGTTTGTGTTTCGGATTTCCTGCTGAANNGNTTNTCGNNTCNNNNNNNNATCCCGATTTCNTTCCGCAGCTNACCTCCCAAN	)'.*.+2,))&&'&*/)-&*-)&.-)&)&),/-&&..)./.,.).*&&,&.&&-)&&&0*&&&&&&&&/32/,01460&&/6/*0*/2/283//36868/&	RG:Z:0
+both_reads_align_clip_marked	163	chr7	302	255	101M	=	1	-201	NCGCGGCATCNCGATTTCTTTCCGCAGCTAACCTCCCGACAGATCGGCAGCGCGTCGTGTAGGTTATTATGGTACATCTTGTCGTGCGGCNAGAGCATACA	&/15445666651/566666553+2/14/&/555512+3/)-'/-&-'*+))*''13+3)'//++''/'))/3+&*5++)&'2+&+/*&-&&*)&-./1'1	RG:Z:0
+read_2_too_many_gaps	163	chr7	302	255	10M1D10M5I76M	=	1	-201	NCGCGGCATCNCGATTTCTTTCCGCAGCTAACCTCCCGACAGATCGGCAGCGCGTCGTGTAGGTTATTATGGTACATCTTGTCGTGCGGCNAGAGCATACA	&/15445666651/566666553+2/14/&/555512+3/)-'/-&-'*+))*''13+3)'//++''/'))/3+&*5++)&'2+&+/*&-&&*)&-./1'1	RG:Z:0
+both_reads_present_only_first_aligns	165	*	0	0	*	chr7	1	0	NCGCGGCATCNCGATTTCTTTCCGCAGCTAACCTCCCGACAGATCGGCAGCGCGTCGTGTAGGTTATTATGGTACATCTTGTCGTGCGGCNAGAGCATACA	&/15445666651/566666553+2/14/&/555512+3/)-'/-&-'*+))*''13+3)'//++''/'))/3+&*5++)&'2+&+/*&-&&*)&-./1'1	RG:Z:0
diff --git a/tool_collections/samtools/bam_to_sam/test-data/bam_to_sam_out2.sam b/tool_collections/samtools/bam_to_sam/test-data/bam_to_sam_out2.sam
new file mode 100644
index 00000000000..3f20214182b
--- /dev/null
+++ b/tool_collections/samtools/bam_to_sam/test-data/bam_to_sam_out2.sam
@@ -0,0 +1,6 @@
+@HD	VN:1.0	SO:coordinate
+@SQ	SN:chr1	LN:101
+@SQ	SN:chr7	LN:404
+@SQ	SN:chr8	LN:202
+@RG	ID:0	SM:Hi,Mom!
+@PG	ID:1	PN:Hey!	VN:2.0
diff --git a/tool_collections/samtools/bam_to_sam/test-data/bam_to_sam_out3.sam b/tool_collections/samtools/bam_to_sam/test-data/bam_to_sam_out3.sam
new file mode 100644
index 00000000000..a9629207b09
--- /dev/null
+++ b/tool_collections/samtools/bam_to_sam/test-data/bam_to_sam_out3.sam
@@ -0,0 +1,8 @@
+both_reads_align_clip_marked	83	chr7	1	255	101M	=	302	201	CAACAGAAGCNGGNATCTGTGTTTGTGTTTCGGATTTCCTGCTGAANNGNTTNTCGNNTCNNNNNNNNATCCCGATTTCNTTCCGCAGCTNACCTCCCAAN	)'.*.+2,))&&'&*/)-&*-)&.-)&)&),/-&&..)./.,.).*&&,&.&&-)&&&0*&&&&&&&&/32/,01460&&/6/*0*/2/283//36868/&	RG:Z:0
+both_reads_present_only_first_aligns	89	chr7	1	255	101M	*	0	0	CAACAGAAGCNGGNATCTGTGTTTGTGTTTCGGATTTCCTGCTGAANNGNTTNTCGNNTCNNNNNNNNATCCCGATTTCNTTCCGCAGCTNACCTCCCAAN	)'.*.+2,))&&'&*/)-&*-)&.-)&)&),/-&&..)./.,.).*&&,&.&&-)&&&0*&&&&&&&&/32/,01460&&/6/*0*/2/283//36868/&	RG:Z:0
+read_2_too_many_gaps	83	chr7	1	255	101M	=	302	201	CAACAGAAGCNGGNATCTGTGTTTGTGTTTCGGATTTCCTGCTGAANNGNTTNTCGNNTCNNNNNNNNATCCCGATTTCNTTCCGCAGCTNACCTCCCAAN	)'.*.+2,))&&'&*/)-&*-)&.-)&)&),/-&&..)./.,.).*&&,&.&&-)&&&0*&&&&&&&&/32/,01460&&/6/*0*/2/283//36868/&	RG:Z:0
+both_reads_align_clip_adapter	147	chr7	16	255	101M	=	21	-96	CAACAGAAGCNGGNATCTGTGTTTGTGTTTCGGATTTCCTGCTGAANNGNTTNTCGNNTCNNNNNNNNATCCCGATTTCNTTCCGCAGCTNACCTCCCAAN	)'.*.+2,))&&'&*/)-&*-)&.-)&)&),/-&&..)./.,.).*&&,&.&&-)&&&0*&&&&&&&&/32/,01460&&/6/*0*/2/283//36868/&	RG:Z:0
+both_reads_align_clip_adapter	99	chr7	21	255	101M	=	16	96	CAACAGAAGCNGGNATCTGTGTTTGTGTTTCGGATTTCCTGCTGAANNGNTTNTCGNNTCNNNNNNNNATCCCGATTTCNTTCCGCAGCTNACCTCCCAAN	)'.*.+2,))&&'&*/)-&*-)&.-)&)&),/-&&..)./.,.).*&&,&.&&-)&&&0*&&&&&&&&/32/,01460&&/6/*0*/2/283//36868/&	RG:Z:0
+both_reads_align_clip_marked	163	chr7	302	255	101M	=	1	-201	NCGCGGCATCNCGATTTCTTTCCGCAGCTAACCTCCCGACAGATCGGCAGCGCGTCGTGTAGGTTATTATGGTACATCTTGTCGTGCGGCNAGAGCATACA	&/15445666651/566666553+2/14/&/555512+3/)-'/-&-'*+))*''13+3)'//++''/'))/3+&*5++)&'2+&+/*&-&&*)&-./1'1	RG:Z:0
+read_2_too_many_gaps	163	chr7	302	255	10M1D10M5I76M	=	1	-201	NCGCGGCATCNCGATTTCTTTCCGCAGCTAACCTCCCGACAGATCGGCAGCGCGTCGTGTAGGTTATTATGGTACATCTTGTCGTGCGGCNAGAGCATACA	&/15445666651/566666553+2/14/&/555512+3/)-'/-&-'*+))*''13+3)'//++''/'))/3+&*5++)&'2+&+/*&-&&*)&-./1'1	RG:Z:0
+both_reads_present_only_first_aligns	165	*	0	0	*	chr7	1	0	NCGCGGCATCNCGATTTCTTTCCGCAGCTAACCTCCCGACAGATCGGCAGCGCGTCGTGTAGGTTATTATGGTACATCTTGTCGTGCGGCNAGAGCATACA	&/15445666651/566666553+2/14/&/555512+3/)-'/-&-'*+))*''13+3)'//++''/'))/3+&*5++)&'2+&+/*&-&&*)&-./1'1	RG:Z:0
diff --git a/tool_collections/samtools/bam_to_sam/tool_dependencies.xml b/tool_collections/samtools/bam_to_sam/tool_dependencies.xml
new file mode 120000
index 00000000000..b11b378d63c
--- /dev/null
+++ b/tool_collections/samtools/bam_to_sam/tool_dependencies.xml
@@ -0,0 +1 @@
+../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/cram_to_bam/.shed.yml b/tool_collections/samtools/cram_to_bam/.shed.yml
new file mode 100644
index 00000000000..a6c37c19b63
--- /dev/null
+++ b/tool_collections/samtools/cram_to_bam/.shed.yml
@@ -0,0 +1,11 @@
+categories:
+- Convert Formats
+- SAM
+description: Converts alignments in CRAM format to BAM format.
+long_description: |
+  This tool uses the SAMtools toolkit to convert CRAM to BAM files.
+name: samtools_cram_to_bam
+owner: devteam
+homepage_url: https://samtools.github.io/
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/cram_to_bam
+type: unrestricted
diff --git a/tool_collections/samtools/cram_to_bam/macros.xml b/tool_collections/samtools/cram_to_bam/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/samtools/cram_to_bam/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/cram_to_bam/samtools_cram_to_bam.xml b/tool_collections/samtools/cram_to_bam/samtools_cram_to_bam.xml
new file mode 100644
index 00000000000..16ad57a4e65
--- /dev/null
+++ b/tool_collections/samtools/cram_to_bam/samtools_cram_to_bam.xml
@@ -0,0 +1,116 @@
+<tool id="samtools_cram_to_bam" name="samtools CRAM to BAM" version="1.2.0">
+    <description>convert CRAM alignments to BAM format</description>
+
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"></expand>
+    <expand macro="version_command"></expand>
+    <expand macro="stdio"></expand>
+
+    <command>
+    <![CDATA[
+        #if str( $input_alignment.metadata.cram_index ) != "None":
+            ln
+                -f
+                -s
+                "${input_alignment.metadata.cram_index}"
+                "${input_alignment}.crai"
+                &&
+        #end if
+        
+        samtools view
+            #if $parameter_regions.target_region == "regions_bed_file"
+                -L "${parameter_regions.regions_bed_file}"
+            #end if
+            
+            -@ \${GALAXY_SLOTS:-2}
+            -b
+            -T "${reference_source.input_reference}"
+            -o "$output_alignment"
+            "$input_alignment"
+            
+            #if $parameter_regions.target_region == "region"
+                "${parameter_regions.region_string}"
+            #end if
+    ]]>
+    </command>
+
+    <inputs>
+        <param name="input_alignment" type="data" format="cram" label="CRAM alignment file"/>
+        <conditional name="reference_source">
+            <param name="reference_source_selector" type="select" label="Load reference genome from">
+                <option value="cached">Local cache</option>
+                <option value="history">History</option>
+            </param>
+            <when value="cached">
+                <param name="input_reference" type="select" format="fasta" label="Genome reference FASTA file">
+                    <options from_data_table="all_fasta">
+                        <filter type="data_meta" ref="input_alignment" key="dbkey" column="1" />
+                    </options>
+                    <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/>
+                </param>
+            </when>
+            <when value="history">
+                <param name="input_reference" type="data" format="fasta" label="Genome reference FASTA file"/>
+            </when>
+        </conditional>
+        <conditional name="parameter_regions">
+            <param name="target_region" type="select" label="Choose conversion within specific genomic region(s)">
+                <option value="entire_input_file">Entire BAM alignment file</option>
+                <option value="region">Specific region</option>
+                <option value="regions_bed_file">List of specific regions (BED file)</option>
+            </param>
+            <when value="entire_input_file" />
+            <when value="region">
+                <param name="region_string" type="text" label="Samtools: region in which pileup is generated" help="e.g. chrX or chr:start-end" />
+            </when>
+            <when value="regions_bed_file">
+                <param name="regions_bed_file" type="data" format="bed" label="only include reads overlapping this BED FILE" help="-L"/>
+            </when>
+        </conditional>
+    </inputs>
+
+    <outputs>
+        <data format="bam" name="output_alignment" label="$tool.name on ${on_string}.bam"></data>
+    </outputs>
+
+    <tests>
+        <test>
+            <param name="input_alignment" value="test.cram" ftype="cram" />
+            <param name="reference_source_selector" value="history" />
+            <param name="input_reference" value="test.fa" />
+
+            <output name="output_alignment" file="test.bam"  compare="sim_size" delta="250" />
+        </test>
+        <test>
+            <param name="input_alignment" value="test.cram" ftype="cram" />
+            <param name="reference_source_selector" value="history" />
+            <param name="input_reference" value="test.fa" />
+            <param name="target_region" value="region" />
+            <param name="region_string" value="CHROMOSOME_I" />
+
+            <output name="output_alignment" file="test.bam"  compare="sim_size" delta="250" />
+        </test>
+        <test>
+            <param name="input_alignment" value="test.cram" ftype="cram" />
+            <param name="reference_source_selector" value="history" />
+            <param name="input_reference" value="test.fa" />
+            <param name="target_region" value="regions_bed_file" />
+            <param name="regions_bed_file" value="test.bed" ftype="bed" />
+
+            <output name="output_alignment" file="test.bam"  compare="sim_size" delta="250" />
+        </test>
+    </tests>
+
+    <help>
+
+**What this tool does**
+
+Converts alignments from the CRAM format to the BAM format using the SAMTools_ toolkit.
+
+.. _SAMTools: http://samtools.sourceforge.net/samtools.shtml
+    </help>
+
+    <expand macro="citations"></expand>
+</tool>
diff --git a/tool_collections/samtools/cram_to_bam/test-data b/tool_collections/samtools/cram_to_bam/test-data
new file mode 120000
index 00000000000..f633e7fbd66
--- /dev/null
+++ b/tool_collections/samtools/cram_to_bam/test-data
@@ -0,0 +1 @@
+../bam_to_cram/test-data
\ No newline at end of file
diff --git a/tool_collections/samtools/cram_to_bam/tool_dependencies.xml b/tool_collections/samtools/cram_to_bam/tool_dependencies.xml
new file mode 120000
index 00000000000..b11b378d63c
--- /dev/null
+++ b/tool_collections/samtools/cram_to_bam/tool_dependencies.xml
@@ -0,0 +1 @@
+../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/macros.xml b/tool_collections/samtools/macros.xml
new file mode 100644
index 00000000000..649aa7bb054
--- /dev/null
+++ b/tool_collections/samtools/macros.xml
@@ -0,0 +1,71 @@
+<macros>
+    <xml name="requirements">
+        <requirements>
+            <requirement type="package" version="1.2">samtools</requirement>
+            <yield/>
+        </requirements>
+    </xml>
+
+    <xml name="citations">
+        <citations>
+            <citation type="bibtex">
+                @misc{SAM_def,
+                title={Definition of SAM/BAM format},
+                url = {https://samtools.github.io/hts-specs/SAMv1.pdf},}
+            </citation>
+            <citation type="doi">10.1093/bioinformatics/btp352</citation>
+            <citation type="doi">10.1093/bioinformatics/btr076</citation>
+            <citation type="doi">10.1093/bioinformatics/btr509</citation>
+            <citation type="bibtex">
+                @misc{Danecek_et_al,
+                Author={Danecek, P., Schiffels, S., Durbin, R.},
+                title={Multiallelic calling model in bcftools (-m)},
+                url = {http://samtools.github.io/bcftools/call-m.pdf},}
+            </citation>
+            <citation type="bibtex">
+                @misc{Durbin_VCQC,
+                Author={Durbin, R.},
+                title={Segregation based metric for variant call QC},
+                url = {http://samtools.github.io/bcftools/rd-SegBias.pdf},}
+            </citation>
+            <citation type="bibtex">
+                @misc{Li_SamMath,
+                Author={Li, H.},
+                title={Mathematical Notes on SAMtools Algorithms},
+                url = {http://www.broadinstitute.org/gatk/media/docs/Samtools.pdf},}
+            </citation>
+            <citation type="bibtex">
+                @misc{SamTools_github,
+                title={SAMTools GitHub page},
+                url = {https://github.com/samtools/samtools},}
+            </citation>
+        </citations>
+    </xml>
+    <xml name="version_command">
+        <version_command>echo $(samtools --version | head -n 1)", "$(samtools --version | grep -o -E "htslib.*?")</version_command>
+    </xml>
+    <xml name="stdio">
+        <stdio>
+            <exit_code range="1:" level="fatal" description="Error" />
+        </stdio>
+    </xml>
+    <token name="@no-chrom-options@">
+-----
+
+.. class:: warningmark
+
+**No options available? How to re-detect metadata**
+
+If you see a &quot;No options available&quot; within the &quot;**Select references (chromosomes and contigs) you would like to restrict bam to**&quot; drop down, you need to re-detect metadata for the dataset you are trying to process. To do this follow these steps:
+
+1. Click on the **pencil** icon adjacent to the dataset in the history
+2. A new menu will appear in the center pane of the interface
+3. Click **Datatype** tab
+4. Set **New Type** to **BAM**
+5. Click **Save**
+
+The medatada will be re-detected and you will be able to see the list of reference sequences in the &quot;**Select references (chromosomes and contigs) you would like to restrict bam to**&quot; drop-down.
+
+    </token>
+
+</macros>
diff --git a/tool_collections/samtools/sam_to_bam/.shed.yml b/tool_collections/samtools/sam_to_bam/.shed.yml
new file mode 100644
index 00000000000..6f0370f3b5f
--- /dev/null
+++ b/tool_collections/samtools/sam_to_bam/.shed.yml
@@ -0,0 +1,11 @@
+categories:
+- SAM
+- Convert Formats
+description: Convert SAM format to BAM format.
+long_description: |
+  This tool uses the SAMtools toolkit to produce an indexed BAM file based on a sorted input SAM file.
+name: sam_to_bam
+owner: devteam
+homepage_url: http://www.htslib.org/
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/sam_to_bam
+type: unrestricted
diff --git a/tool_collections/samtools/sam_to_bam/macros.xml b/tool_collections/samtools/sam_to_bam/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/samtools/sam_to_bam/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/sam_to_bam/sam_to_bam.xml b/tool_collections/samtools/sam_to_bam/sam_to_bam.xml
new file mode 100644
index 00000000000..de35dbe6998
--- /dev/null
+++ b/tool_collections/samtools/sam_to_bam/sam_to_bam.xml
@@ -0,0 +1,89 @@
+<tool id="sam_to_bam" name="SAM-to-BAM" version="2.1">
+  <description>convert SAM to BAM</description>
+  <macros>
+    <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"></expand>
+  <expand macro="stdio"></expand>
+  <expand macro="version_command"></expand>
+  <command>
+    <![CDATA[
+        #if $source.index_source == "history":
+          ln -s $source.ref_file input.fa &&
+          samtools faidx input.fa &&
+        #else
+          ln -s ${source.index.fields.path} input.fa &&
+          ln -s ${source.index.fields.path}.fai input.fa.fai &&
+        #end if
+        samtools view -b -@ \${GALAXY_SLOTS:-1} -t input.fa.fai "$input1" | samtools sort -O bam -@ \${GALAXY_SLOTS:-1} -o "$output1" -T temp
+    ]]>
+    </command>
+    <inputs>
+        <conditional name="source">
+            <param label="Choose the source for the reference genome" name="index_source" type="select">
+                <option value="cached">Use a built-in genome</option>
+                <option value="history">Use a genome from the history</option>
+            </param>
+            <when value="cached">
+                <param format="sam" label="SAM file to convert" metadata_name="dbkey" name="input1" type="data">
+                    <validator type="unspecified_build" />
+                    <validator message="Sequences are not currently available for the specified build." metadata_column="1" metadata_name="dbkey" table_name="fasta_indexes" type="dataset_metadata_in_data_table" />
+                </param>
+                <param label="Using reference genome" name="index" type="select">
+                    <options from_data_table="fasta_indexes">
+                        <filter column="1" key="dbkey" ref="input1" type="data_meta" />
+                        <validator message="No reference genome is available for the build associated with the selected input dataset" type="no_options" />
+                    </options>
+                </param>
+            </when>
+            <when value="history">
+                <param format="sam" label="SAM file to convert" name="input1" type="data" />
+                <param format="fasta" label="Using reference file" metadata_name="dbkey" name="ref_file" type="data" />
+            </when>
+        </conditional>
+    </inputs>
+    <outputs>
+        <data format="bam" label="${tool.name} on ${on_string}: converted BAM" name="output1">
+            <actions>
+                <conditional name="source.index_source">
+                    <when value="cached">
+                        <action name="dbkey" type="metadata">
+                            <option name="source.input1" param_attribute="dbkey" type="from_param" />
+                        </action>
+                    </when>
+                    <when value="history">
+                        <action name="dbkey" type="metadata">
+                            <option name="source.ref_file" param_attribute="dbkey" type="from_param" />
+                        </action>
+                    </when>
+                </conditional>
+            </actions>
+        </data>
+    </outputs>
+    <tests>
+        <test>
+            <param name="index_source" value="history" />
+            <param name="input1" ftype="sam" value="sam_to_bam_in1.sam" />
+            <param dbkey="equCab2" ftype="fasta" name="ref_file" value="chr_m.fasta" />
+            <output file="sam_to_bam_out1.bam" ftype="bam" name="output1" />
+        </test>
+        <test>
+            <param name="index_source" value="history" />
+            <param name="input1" ftype="sam" value="sam_to_bam_noheader_in2.sam" />
+            <param dbkey="equCab2" ftype="fasta" name="ref_file" value="chr_m.fasta" />
+            <output file="sam_to_bam_out3.bam" ftype="bam" name="output1" />
+        </test>
+    </tests>
+    <help>
+<![CDATA[
+**What it does**
+
+Converts SAM dataset into its binary, BAM, representation using ``samtools sort`` and ``view`` commands::
+
+  samtools sort -O bam -o sorted_input.bam [INPUT SAM] 
+  samtools view -b -h -o -T [REFERENCE GENOME] [OUTPUT BAM] sorted_input.bam
+
+]]>
+  </help>
+  <expand macro="citations"></expand>
+</tool>
diff --git a/tool_collections/samtools/sam_to_bam/test-data/cached_locally/all_fasta.loc b/tool_collections/samtools/sam_to_bam/test-data/cached_locally/all_fasta.loc
new file mode 100644
index 00000000000..525bdb3c42d
--- /dev/null
+++ b/tool_collections/samtools/sam_to_bam/test-data/cached_locally/all_fasta.loc
@@ -0,0 +1 @@
+chrM	chr_m	Horse (Equus caballus): equCab2 chrM	${__HERE__}/chrM.fasta
diff --git a/tool_collections/samtools/sam_to_bam/test-data/cached_locally/chr_m.fasta b/tool_collections/samtools/sam_to_bam/test-data/cached_locally/chr_m.fasta
new file mode 120000
index 00000000000..d467c4ae9d8
--- /dev/null
+++ b/tool_collections/samtools/sam_to_bam/test-data/cached_locally/chr_m.fasta
@@ -0,0 +1 @@
+../chr_m.fasta
\ No newline at end of file
diff --git a/tool_collections/samtools/sam_to_bam/test-data/cached_locally/chr_m.fasta.fai b/tool_collections/samtools/sam_to_bam/test-data/cached_locally/chr_m.fasta.fai
new file mode 120000
index 00000000000..9ec361ce008
--- /dev/null
+++ b/tool_collections/samtools/sam_to_bam/test-data/cached_locally/chr_m.fasta.fai
@@ -0,0 +1 @@
+../chr_m.fasta.fai
\ No newline at end of file
diff --git a/tool_collections/samtools/sam_to_bam/test-data/cached_locally/fasta_indexes.loc b/tool_collections/samtools/sam_to_bam/test-data/cached_locally/fasta_indexes.loc
new file mode 100644
index 00000000000..525bdb3c42d
--- /dev/null
+++ b/tool_collections/samtools/sam_to_bam/test-data/cached_locally/fasta_indexes.loc
@@ -0,0 +1 @@
+chrM	chr_m	Horse (Equus caballus): equCab2 chrM	${__HERE__}/chrM.fasta
diff --git a/tool_collections/samtools/sam_to_bam/test-data/chr_m.fasta b/tool_collections/samtools/sam_to_bam/test-data/chr_m.fasta
new file mode 100644
index 00000000000..0ebaea323f0
--- /dev/null
+++ b/tool_collections/samtools/sam_to_bam/test-data/chr_m.fasta
@@ -0,0 +1,335 @@
+>chrM
+GTTAATGTAGCTTAATAATATAAAGCAAGGCACTGAAAATGCCTAGATGA
+GTATTCTTACTCCATAAACACATAGGCTTGGTCCTAGCCTTTTTATTAGT
+TATTAATAGAATTACACATGCAAGTATCCGCACCCCAGTGAGAATGCCCT
+CTAAATCACGTCTCTACGATTAAAAGGAGCAGGTATCAAGCACACTAGAA
+AGTAGCTCATAACACCTTGCTCAGCCACACCCCCACGGGACACAGCAGTG
+ATAAAAATTAAGCTATGAACGAAAGTTCGACTAAGTCATATTAAATAAGG
+GTTGGTAAATTTCGTGCCAGCCACCGCGGTCATACGATTAACCCAAATTA
+ATAAATCTCCGGCGTAAAGCGTGTCAAAGACTAATACCAAAATAAAGTTA
+AAACCCAGTTAAGCCGTAAAAAGCTACAACCAAAGTAAAATAGACTACGA
+AAGTGACTTTAATACCTCTGACTACACGATAGCTAAGACCCAAACTGGGA
+TTAGATACCCCACTATGCTTAGCCCTAAACTAAAATAGCTTACCACAACA
+AAGCTATTCGCCAGAGTACTACTAGCAACAGCCTAAAACTCAAAGGACTT
+GGCGGTGCTTTACATCCCTCTAGAGGAGCCTGTTCCATAATCGATAAACC
+CCGATAAACCCCACCATCCCTTGCTAATTCAGCCTATATACCGCCATCTT
+CAGCAAACCCTAAACAAGGTACCGAAGTAAGCACAAATATCCAACATAAA
+AACGTTAGGTCAAGGTGTAGCCCATGGGATGGAGAGAAATGGGCTACATT
+TTCTACCCTAAGAACAAGAACTTTAACCCGGACGAAAGTCTCCATGAAAC
+TGGAGACTAAAGGAGGATTTAGCAGTAAATTAAGAATAGAGAGCTTAATT
+GAATCAGGCCATGAAGCGCGCACACACCGCCCGTCACCCTCCTTAAATAT
+CACAAATCATAACATAACATAAAACCGTGACCCAAACATATGAAAGGAGA
+CAAGTCGTAACAAGGTAAGTATACCGGAAGGTGTACTTGGATAACCAAAG
+TGTAGCTTAAACAAAGCATCCAGCTTACACCTAGAAGATTTCACTCAAAA
+TGAACACTTTGAACTAAAGCTAGCCCAAACAATACCTAATTCAATTACCC
+TTAGTCACTTAACTAAAACATTCACCAAACCATTAAAGTATAGGAGATAG
+AAATTTTAACTTGGCGCTATAGAGAAAGTACCGTAAGGGAACGATGAAAG
+ATGCATTAAAAGTACTAAACAGCAAAGCTTACCCCTTTTACCTTTTGCAT
+AATGATTTAACTAGAATAAACTTAGCAAAGAGAACTTAAGCTAAGCACCC
+CGAAACCAGACGAGCTACCTATGAACAGTTACAAATGAACCAACTCATCT
+ATGTCGCAAAATAGTGAGAAGATTCGTAGGTAGAGGTGAAAAGCCCAACG
+AGCCTGGTGATAGCTGGTTGTCCAGAAACAGAATTTCAGTTCAAATTTAA
+ATTTACCTAAAAACTACTCAATTCTAATGTAAATTTAAATTATAGTCTAA
+AAAGGTACAGCTTTTTAGATACAGGTTACAACCTTCATTAGAGAGTAAGA
+ACAAGATAAACCCATAGTTGGCTTAAAAGCAGCCATCAATTAAGAAAGCG
+TTCAAGCTCAACGACACATCTATCTTAATCCCAACAATCAACCCAAACTA
+ACTCCTAATCTCATACTGGACTATTCTATCAACACATAGAAGCAATAATG
+TTAATATGAGTAACAAGAATTATTTCTCCTTGCATAAGCTTATATCAGAA
+CGAATACTCACTGATAGTTAACAACAAGATAGGGATAATCCAAAAACTAA
+TCATCTATTTAAACCATTGTTAACCCAACACAGGCATGCATCTATAAGGA
+AAGATTAAAAGAAGTAAAAGGAACTCGGCAAACACAAACCCCGCCTGTTT
+ACCAAAAACATCACCTCTAGCATTTCCAGTATTAGAGGCACTGCCTGCCC
+AGTGACATCTGTTtaaacggccgcggtatcctaaccgtgcaaaggtagca
+taatcacttgttccctaaatagggacttgtatgaatggccacacgagggt
+tttactgtctcttacttccaatcagtgaaattgaccttcccgtgaagagg
+cgggaatgactaaataagacgagaagaccctatggagcttTAATTAACTG
+ATTCACAAAAAACAACACACAAACCTTAACCTTCAGGGACAACAAAACTT
+TTGATTGAATCAGCAATTTCGGTTGGGGTGACCTCGGAGAACAAAACAAC
+CTCCGAGTGATTTAAATCCAGACTAACCAGTCAAAATATATAATCACTTA
+TTGATCCAAACCATTGATCAACGGAACAAGTTACCCTAGGGATAACAGCG
+CAATCCTATTCCAGAGTCCATATCGACAATTAGGGTTTACGACCTCGATG
+TTGGATCAAGACATCCTAATGGTGCAACCGCTATTAAGGGTTCGTTTGTT
+CAACGATTAAAGTCTTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGT
+CGGTTTCTATCTATTCTATACTTTTCCCAGTACGAAAGGACAAGAAAAGT
+AGGGCCCACTTTACAAGAAGCGCCCTCAAACTAATAGATGACATAATCTA
+AATCTAACTAATTTATAACTTCTACCGCCCTAGAACAGGGCTCgttaggg
+tggcagagcccggaaattgcataaaacttaaacctttacactcagaggtt
+caactcctctccctaacaacaTGTTCATAATTAACGTCCTCCTCCTAATT
+GTCCCAATCTTGCTCGCCGTAGCATTCCTCACACTAGTTGAACGAAAAGT
+CTTAGGCTATATGCAACTTCGCAAAGGACCCAACATCGTAGGCCCCTATG
+GCCTACTACAACCTATTGCCGATGCCCTCAAACTATTTATCAAAGAGCCA
+CTACAACCACTAACATCATCGACATCCATATTCATCATCGCACCAATCCT
+AGCCCTAACCCTGGCCTTAACCATATGAATCCCTCTGCCCATACCATACC
+CACTAATCAACATAAACCTAGGAATTCTATTCATACTAGCCATGTCCAGC
+CTAGCTGTCTACTCAATCCTTTGATCAGGATGGGCCTCAAACTCAAAATA
+CGCCCTAATTGGAGCTCTACGAGCAGTAGCACAAACCATCTCATACGAAG
+TAACTCTAGCAATCATCCTACTCTCAGTCCTCCTAATAAGCGGATCATTC
+ACATTATCAACACTTATTATTACCCAAGAATACCTCTGATTAATCTTCCC
+ATCATGACCCTTAGCCATAATGTGATTCATCTCAACATTAGCCGAAACCA
+ACCGAGCTCCATTTGACCTAACAGAAGGAGAATCAGAACTCGTCTCTGGA
+TTCAACGTTGAATACGCAGCCGGCCCATTTGCTCTATTCTTCCTAGCAGA
+ATACGCAAACATCATCATGATAAACATCTTCACAACAACCCTATTTCTAG
+GAGCATTTCACAACCCCTACCTGCCAGAACTCTACTCAATTAATTTCACC
+ATTAAAGCTCTCCTTCTAACATGTTCCTTCCTATGAATCCGAGCATCCTA
+CCCACGATTCCGATATGACCAACTTATACACCTCCTATGAAAGAACTTCC
+TACCACTCACACTAGCCCTCTGCATATGACACGTCTCACTTCCAATCATA
+CTATCCAGCATCCCACCACAAACATAGGAAATATGTCTGACAAAAGAGTT
+ACTTTGATAGAGTAAAACATAGAGGCTCAAACCCTCTTATTTctagaact
+acaggaattgaacctgctcctgagaattcaaaatcctccgtgctaccgaa
+ttacaccatgtcctaCAAGTAAGGTCAGCTAAATAAGCTATCGGGCCCAT
+ACCCCGAAAATGTTGGATTACACCCTTCCCGTACTAATAAATCCCCTTAT
+CTTCACAACTATTCTAATAACAGTTCTTCTAGGAACTATAATCGTTATAA
+TAAGCTCACACTGACTAATAATCTGAATCGGATTTGAAATAAATCTACTA
+GCCATTATCCCTATCCTAATAAAAAAGTACAATCCCCGAACCATAGAAGC
+CTCCACCAAATATTTTCTAACCCAAGCCACCGCATCAATACTCCTCATAA
+TAGCGATCATCATTAACCTCATACACTCAGGCCAATGAACAATCACAAAA
+GTCTTCAACCCCACAGCGTCCATCATTATAACTTCAGCTCTCGCCATAAA
+ACTTGGACTCACACCATTCCACTTCTGAGTACCCGAAGTCACACAGGGCA
+TCTCATTAACATCAGGTCTCATCCTACTTACATGACAAAAACTAGCCCCA
+ATATCAATCCTATATCAAATCTCACCCTCAATTAACCTAAATATCTTATT
+AACTATAGCCGTACTGTCAATCCTAGTAGGAGGCTGAGGCGGTCTCAACC
+AAACCCAACTACGAAAAATCATAGCATACTCGTCAATCGCGCATATAGGA
+TGAATAACAGCTGTCCTAGTATATAACCCAACACTAACAATACTAAACAT
+ATTAATTTACATTATAATAACACTCACAATATTCATACTATTTATCCACA
+GCTCCTCTACTACAACACTATCACTCTCCCACACATGAAACAAAATACCT
+CTAACCACTACACTAATCTTAATTACCTTACTATCCATAGGAGGCCTCCC
+CCCACTATCAGGATTCATACCCAAATGAATAATCATTCAAGAGCTCACCA
+AAAATAGCAGCATCATCCTCCCCACACTAATAGCCATTATAGCACTACTC
+AACCTCTACTTCTACATACGACTAACCTATTCCACCTCACTGACCATATT
+CCCATCCACAAACAACATAAAAATAAAATGACAATTCGAAACCAAACGAA
+TTACTCTCTTACCCCCGTTAATTGTTATATCCTCCCTACTCCTCCCCCTA
+ACCCCCATACTATCAATTTTGGACTAGGAATTTAGGTTAACATCCCAGAC
+CAAGAGCCTTCAAAGCTCTAAGCAAGTGAATCCACTTAATTCCTGCATAC
+TAAGGACTGCGAGACTCTATCTCACATCAATTGAACGCAAATCAAACTCT
+TTTATTAAGCTAAGCCCTTACTAGATTGGTGGGCTACCATCCCACGAAAT
+TTTAGTTAACAGCTAAATACCCTAATCAACTGGCTTCAATCTACTTCTCC
+CGCCGCCTAGAAAAAAAGGCGGGAGAAGCCCCGGCAGAAATTGAAGCTGC
+TCCTTTGAATTTGCAATTCAATGTGAAAATTCACCACGGGACTTGATAAG
+AAGAGGATTCCAACCCCTGTCTTTAGATTTACAGTCTAATGCTTACTCAG
+CCATCTTACCTATGTTCATCAACCGCTGACTATTTTCAACTAACCACAAA
+GACATCGGCACTCTGTACCTCCTATTCGGCGCTTGAGCTGGAATAGTAGG
+AACTGCCCTAAGCCTCCTAATCCGTGCTGAATTAGGCCAACCTGGGACCC
+TACTAGGAGATGATCAGATCTACAATGTCATTGTAACCGCCCATGCATTC
+GTAATAATTTTCTTTATGGTCATACCCATTATAATCGGAGGATTCGGAAA
+CTGATTAGTCCCCCTGATAATTGGAGCACCTGATATAGCTTTCCCCCGAA
+TAAACAACATAAGCTTCTGATTACTTCCCCCATCATTCCTACTTCTTCTC
+GCTTCCTCAATAATTGAAGCAGGTGCCGGAACAGGCTGAACCGTATATCC
+TCCTCTAGCTGGAAATCTGGCGCATGCAGGAGCCTCTGTTGACTTAACCA
+TTTTCTCTCTCCACCTAGCTGGGGTGTCCTCGATTTTAGGTGCCATCAAC
+TTTATTACCACAATCATTAACATAAAACCACCAGCCCTATCCCAATATCA
+AACCCCCCTATTCGTTTGATCTGTCCTTATTACGGCAGTACTCCTTCTCC
+TAGCCCTCCCGGTCCTAGCAGCAGGCATTACCATGCTTCTCACAGACCGT
+AACCTGAACACTACTTTCTTCGACCCCGCAGGAGGAGGGGATCCAATCCT
+TTATCAACACCTATTCTGATTCTTCGGACACCCCGAAGTCTATATTCTTA
+TCCTACCAGGCTTCGGTATAATCTCACACATCGTCACATACTACTCAGGT
+AAAAAGGAACCTTTTGGCTACATGGGTATAGTGTGAGCTATAATATCCAT
+TGGCTTTCTAGGCTTCATCGTATGGGCTCACCACATGTTTACAGTAGGGA
+TAGACGTTGACACACGAGCATACTTCACATCAGCTACCATAATCATCGCT
+ATCCCTACTGGTGTAAAAGTATTCAGCTGACTAGCCACCCTGCACGGAGG
+AAATATCAAATGATCTCCAGCTATACTCTGAGCTCTAGGCTTCATCTTCT
+TATTCACAGTAGGAGGTCTAACAGGAATCGTCCTAGCTAACTCATCCCTA
+GATATTGTTCTCCACGATACTTATTATGTAGTAGCACATTTCCATTATGT
+CCTGTCTATAGGAGCAGTCTTCGCCATTATGGGGGGATTTGTACACTGAT
+TCCCTCTATTCTCAGGATACACACTCAACCAAACCTGAGCAAAAATCCAC
+TTTACAATTATATTCGTAGGGGTAAATATAACCTTCTTCCCACAACATTT
+CCTTGGCCTCTCAGGAATGCCACGACGCTATTCTGATTATCCAGACGCAT
+ATACAACATGAAATACCATCTCATCCATAGGATCTTTTATCTCACTTACA
+GCAGTGATACTAATAATTTTCATAATTTGAGAAGCGTTCGCATCCAAACG
+AGAAGTGTCTACAGTAGAATTAACCTCAACTAATCTGGAATGACTACACG
+GATGCCCCCCACCATACCACACATTTGAAGAACCCACCTACGTAAACCTA
+AAAtaagaaaggaaggaatcgaaccccctctaactggtttcaagccaata
+tcataaccactatgtctttctcCATCAATTGAGGTATTAGTAAAAATTAC
+ATGACTTTGTCAAAGTTAAATTATAGGTTAAACCCCTATATACCTCTATG
+GCCTACCCCTTCCAACTAGGATTCCAAGACGCAACATCCCCTATTATAGA
+AGAACTCCTACACTTCCACGACCACACACTAATAATCGTATTCCTAATTA
+GCTCTCTAGTATTATATATTATCTCATCAATACTAACAACTAAATTAACC
+CATACCAGCACCATAGATGCTCAAGAAGTAGAGACAATTTGAACGATTTT
+ACCAGCCATCATCCTTATTCTAATCGCCCTCCCATCCCTACGAATTCTAT
+ATATAATAGATGAAATCAATAATCCGTCCCTCACAGTCAAAACAATAGGC
+CACCAATGATACTGAAGCTACGAGTATACCGATTACGAAGACTTGACCTT
+TGACTCCTACATGATCCCCACATCAGACCTAAAACCAGGAGAATTACGTC
+TTCTAGAAGTCGACAATCGAGTGGTTCTCCCCATAGAAATAACCATCCGA
+ATGCTAATTTCATCCGAAGACGTCCTACACTCATGAGCTGTGCCCTCCCT
+AGGCCTAAAAACAGACGCTATCCCTGGGCGCCTAAATCAGACAACTCTCG
+TGGCCTCTCGACCAGGACTTTACTACGGTCAATGCTCAGAGATCTGCGGA
+TCAAACCACAGCTTTATACCAATTGTCCTTGAACTAGTTCCACTGAAACA
+CTTCGAAGAATGATCTGCATCAATATTATAAAGTCACTAAGAAGCTATTA
+TAGCATTAACCTTTTAAGTTAAAGATTGAGGGTTCAACCCCCTCCCTAGT
+GATATGCCACAGTTGGATACATCAACATGATTTATTAATATCGTCTCAAT
+AATCCTAACTCTATTTATTGTATTTCAACTAAAAATCTCAAAGCACTCCT
+ATCCGACACACCCAGAAGTAAAGACAACCAAAATAACAAAACACTCTGCC
+CCTTGAGAATCAAAATGAACGAAAATCTATTCGCCTCTTTCGCTACCCCA
+ACAATAGTAGGCCTCCCTATTGTAATTCTGATCATCATATTTCCCAGCAT
+CCTATTCCCCTCACCCAACCGACTAATCAACAATCGCCTAATCTCAATTC
+AACAATGGCTAGTCCAACTTACATCAAAACAAATAATAGCTATCCATAAC
+AGCAAAGGACAAACCTGAACTCTTATACTCATATCACTGATCCTATTCAT
+TGGCTCAACAAACTTATTAGGCCTACTACCTCACTCATTTACACCAACAA
+CACAACTATCAATAAACCTAGGCATAGCTATTCCCCTATGGGCAGGGACA
+GTATTCATAGGCTTTCGTCACAAAACAAAAGCAGCCCTAGCCCACTTTCT
+ACCTCAAGGGACGCCCATTTTCCTCATCCCCATACTAGTAATTATCGAGA
+CTATCAGCCTATTTATTCAACCTGTAGCCCTAGCCGTGCGGCTAACCGCT
+AACATTACCGCCGGACACCTCCTAATACACCTCATCGGAGGGGCAACACT
+AGCCCTCATAAGCATCAGCCCCTCAACAGCCCTTATTACGTTTATCATCC
+TAATTCTACTAACTATCCTCGAATTCGCAGTAGCTATAATCCAAGCCTAC
+GTATTCACTCTCCTGGTAAGCCTTTACTTACACGACAACACCTAATGACC
+CACCAAACCCACGCTTACCACATAGTAAACCCCAGCCCATGACCACTTAC
+AGGAGCCCTATCAGCCCTCCTGATAACATCAGGACTAGCCATGTGATTTC
+ACTTTAACTCAACCTTACTTCTAGCTATAGGGCTATTAACTAACATCCTT
+ACCATATATCAATGATGACGAGACATCATCCGAGAAAGCACATTCCAAGG
+CCATCACACATCAATCGTTCAAAAGGGACTCCGATATGGCATAATCCTTT
+TTATTATCTCAGAAGTCTTCTTCTTCTCTGGCTTCTTCTGAGCCTTTTAC
+CACTCAAGCCTAGCCCCCACACCCGAACTAGGCGGCTGCTGACCACCCAC
+AGGTATCCACCCCTTAAACCCCCTAGAAGTCCCCTTACTCAACACCTCAG
+TGCTCCTAGCATCTGGAGTCTCTATCACCTGAGCCCACCATAGCCTAATA
+GAAGGAAACCGTAAAAATATGCTCCAAGGCCTATTCATCACAATTTCACT
+AGGCGTATACTTCACCCTTCTCCAAGCCTCAGAATACTATGAAGCCTCAT
+TTACTATTTCAGATGGAGTATACGGATCAACATTTTTCGTAGCAACAGGG
+TTCCACGGACTACACGTAATTATCGGATCTACCTTCCTCATTGTATGTTT
+CCTACGCCAACTAAAATTCCACTTTACATCCAGCCACCACTTCGGATTCG
+AAGCAGCCGCTTGATACTGACACTTCGTCGACGTAGTCTGACTATTCTTG
+TACGTCTCTATTTATTGATGAGGATCCTATTCTTTTAGTATTGACCAGTA
+CAATTGACTTCCAATCAATCAGCTTCGGTATAACCCGAAAAAGAATAATA
+AACCTCATACTGACACTCCTCACTAACACATTACTAGCCTCGCTACTCGT
+ACTCATCGCATTCTGACTACCACAACTAAACATCTATGCAGAAAAAACCA
+GCCCATATGAATGCGGATTTGACCCTATAGGGTCAGCACGCCTCCCCTTC
+TCAATAAAATTTTTCTTAGTGGCCATTACATTTCTGCTATTCGACTTAGA
+AATTGCCCTCCTATTACCCCTTCCATGAGCATCCCAAACAACTAACCTAA
+ACACTATACTTATCATAGCACTAGTCCTAATCTCTCTTCTAGCCATCAGC
+CTAGCCTACGAATGAACCCAAAAAGGACTAGAATGAACTGAGTATGGTAA
+TTAGTTTAAACCAAAACAAATGATTTCGACTCATTAAACTATGATTAACT
+TCATAATTACCAACATGTCACTAGTCCATATTAATATCTTCCTAGCATTC
+ACAGTATCCCTCGTAGGCCTACTAATGTACCGATCCCACCTAATATCCTC
+ACTCCTATGCCTAGAAGGAATAATACTATCACTATTCGTCATAGCAACCA
+TAATAGTCCTAAACACCCACTTCACACTAGCTAGTATAATACCTATCATC
+TTACTAGTATTTGCTGCCTGCGAACGAGCTCTAGGATTATCCCTACTAGT
+CATAGTCTCCAATACTTATGGAGTAGACCACGTACAAAACCTTAACCTCC
+TCCAATGCTAAAAATTATCATTCCCACAATCATACTTATGCCCCTTACAT
+GACTATCAAAAAAGAATATAATCTGAATCAACACTACAACCTATAGTCTA
+TTAATCAGCCTTATCAGCCTATCCCTCCTAAACCAACCTAGCAACAATAG
+CCTAAACTTCTCACTAATATTCTTCTCCGATCCCCTATCAGCCCCACTTC
+TGGTGTTGACAACATGACTACTGCCACTAATACTCATAGCCAGCCAACAC
+CATCTATCTAAGGAACCACTAATCCGAAAAAAACTCTACATCACCATGCT
+AACCATACTTCAAACTTTCCTAATCATGACTTTTACCGCCACAGAACTAA
+TCTCCTTCTACATCCTATTTGAAGCCACATTAGTTCCAACACTAATTATC
+ATCACCCGCTGAGGCAACCAAACAGAACGCCTGAACGCAGGCCTCTACTT
+CCTATTCTACACACTAATAGGTTCCCTCCCACTCTTAGTTGCACTAATCT
+CTATCCAAAACCTAACAGGCTCACTAAACTTCCTATTAATTCAATACTGA
+AACCAAGCACTACCCGACTCTTGATCCAATATTTTCCTATGACTAGCATG
+TATAATAGCATTCATAGTCAAAATACCGGTATATGGTCTTCACCTCTGAC
+TCCCAAAAGCCCATGTAGAAGCCCCAATTGCCGGATCCATAGTGCTAGCA
+GCCATTCTACTAAAACTAGGAGGCTACGGAATACTACGAATTACAACAAT
+ACTAAACCCCCAAACTAGCTTTATAGCCTACCCCTTCCTCATACTATCCC
+TGTGAGGAATAATCATAACTAGTTCCATCTGCTTGCGACAAACCGATCTA
+AAATCACTTATTGCATACTCCTCTGTCAGCCACATAGCCCTAGTAATCGT
+AGCCGTCCTCATCCAAACACCATGAAGTTATATAGGAGCTACAGCCCTAA
+TAATCGCTCACGGCCTTACATCATCAATACTATTCTGCCTGGCAAACTCA
+AATTACGAACGTACCCATAGCCGAACTATAATCCTAGCCCGCGGGCTTCA
+AACACTTCTTCCCCTTATAGCAGCCTGATGACTATTAGCCAGCCTAACCA
+ACCTGGCCCTCCCTCCCAGCATTAACCTAATTGGAGAGCTATTCGTAGTA
+ATATCATCATTCTCATGATCAAATATTACCATTATCCTAATAGGAGCCAA
+TATCACCATCACCGCCCTCTACTCCCTATACATACTAATCACAACACAAC
+GAGGGAAATACACACACCATATCAACAGCATTAAACCTTCATTTACACGA
+GAAAACGCACTCATGGCCCTCCACATGACTCCCCTACTACTCCTATCACT
+TAACCCTAAAATTATCCTAGGCTTTACGTACTGTAAATATAGTTTAACAA
+AAACACTAGATTGTGGATCTAGAAACAGAAACTTAATATTTCTTATTTAC
+CGAGAAAGTATGCAAGAACTGCTAATTCATGCCCCCATGTCCAACAAACA
+TGGCTCTCTCAAACTTTTAAAGGATAGGAGCTATCCGTTGGTCTTAGGAA
+CCAAAAAATTGGTGCAACTCCAAATAAAAGTAATCAACATGTTCTCCTCC
+CTCATACTAGTTTCACTATTAGTACTAACCCTCCCAATCATATTATCAAT
+CTTCAATACCTACAAAAACAGCACGTTCCCGCATCATGTAAAAAACACTA
+TCTCATATGCCTTCATTACTAGCCTAATTCCCACTATAATATTTATTCAC
+TCTGGACAAGAAACAATTATCTCAAACTGACACTGAATAACCATACAAAC
+CCTCAAACTATCCCTAAGCTTCAAACTAGATTACTTCTCAATAATTTTCG
+TACCAGTAGCCCTATTCGTAACATGATCTATTATGGAATTCTCCCTATGA
+TACATGCACTCAGATCCTTACATTACTCGATTTTTTAAATACTTACTTAC
+ATTCCTCATCACTATAATAATTCTAGTCACAGCTAACAACCTTTTCCAAC
+TGTTCATCGGATGGGAGGGAGTAGGCATCATGTCATTCTTACTAATCGGA
+TGATGATACGGCCGAACAGATGCCAACACCGCGGCCCTTCAAGCAATCCT
+TTATAACCGCATCGGGGATATCGGCTTCATCATGGCCATAGCCTGATTCC
+TATTCAACACCAACACATGAGACCTCCAACAAATCTTCATACTCGACCCC
+AACCTTACCAACCTCCCGCTCCTAGGCCTCCTCCTAGCCGCAACTGGCAA
+ATCCGCTCAATTTGGACTCCACCCATGACTTCCTTCAGCCATAGAGGGCC
+CTACACCAGTCTCAGCCCTACTCCACTCCAGCACAATAGTTGTAGCAGGC
+GTCTTCCTGCTAATCCGCTTCCATCCACTAATAGAAAACAACAAAACAAT
+CCAGTCACTTACCCTATGCCTAGGAGCCATCACCACACTATTCACAGCAA
+TCTGCGCACTCACTCAAAACGATATCAAAAAAATCATTGCTTTCTCCACC
+TCCAGCCAACTAGGCCTGATAATCGTAACCATCGGTATCAATCAACCCTA
+CCTAGCATTCCTCCACATTTGCACTCACGCATTCTTCAAAGCTATACTAT
+TTATATGTTCCGGATCCATTATCCACAGCCTAAATGACGAGCAAGATATC
+CGAAAAATAGGCGGACTATTTAATGCAATACCCTTCACCACCACATCTCT
+AATTATTGGCAGCCTTGCACTCACCGGAATTCCTTTCCTCACAGGCTTCT
+ACTCCAAAGACCTCATCATCGAAACCGCCAACACATCGTACACCAACGCC
+TGAGCCCTACTAATAACTCTCATTGCCACATCCCTCACAGCTGTCTACAG
+TACCCGAATCATCTTCTTTGCACTCCTAGGGCAACCCCGCTTCCTCCCTC
+TGACCTCAATCAACGAAAATAACCCCTTTCTAATTAACTCCATCAAACGC
+CTCTTAATTGGCAGCATTTTTGCCGGATTCTTCATCTCCAACAATATCTA
+CCCCACAACCGTCCCAGAAATAACCATACCTACTTACATAAAACTCACCG
+CCCTCGCAGTAACCATCCTAGGATTTACACTAGCCCTAGAACTAAGCTTG
+ATAACCCATAACTTAAAACTAGAACACTCCACCAACGTATTCAAATTCTC
+CAACCTCCTAGGATACTACCCAACAATTATACACCGACTCCCACCGCTCG
+CTAACCTATCAATAAGCCAAAAATCAGCATCACTTCTACTAGACTCAATC
+TGACTAGAAAACATCCTGCCAAAATCTATCTCCCAGTTCCAAATAAAAAC
+CTCGATCCTAATTTCCACCCAAAAAGGACAAATCAAATTATATTTCCTCT
+CATTCCTCATCACCCTTACCCTAAGCATACTACTTTTTAATCTCCACGAG
+TAACCTCTAAAATTACCAAGACCCCAACAAGCAACGATCAACCAGTCACA
+ATCACAACCCAAGCCCCATAACTATACAATGCAGCAGCCCCTATAATTTC
+CTCACTAAACGCCCCAGAATCTCCAGTATCATAAATAGCTCAAGCCCCCA
+CACCACTAAACTTAAACACTACCCCCACTTCCTCACTCTTCAGAACATAT
+AAAACCAACATAACCTCCATCAACAACCCTAAAAGAAATACCCCCATAAC
+AGTCGTATTAGACACCCATACCTCAGGATACTGCTCAGTAGCCATAGCCG
+TTGTATAACCAAAAACAACCAACATTCCTCCCAAATAAATCAAAAACACC
+ATCAACCCCAAAAAGGACCCTCCAAAATTCATAATAATACCACAACCTAC
+CCCTCCACTTACAATCAGCACTAAACCCCCATAAATAGGTGAAGGTTTTG
+AAGAAAACCCCACAAAACTAACAACAAAAATAACACTCAAAATAAACACA
+ATATATGTCATCATTATTCCCACGTGGAATCTAACCACGACCAATGACAT
+GAAAAATCATCGTTGTATTTCAACTATAAGAACACCAATGACAAACATCC
+GGAAATCTCACCCACTAATTAAAATCATCAATCACTCTTTTATTGACCTA
+CCAGCCCCCTCAAACATTTCATCATGATGAAACTTCGGCTCCCTCCTAGG
+AATCTGCCTAATCCTCCAAATCTTAACAGGCCTATTCCTAGCCATACACT
+ACACATCAGACACGACAACTGCCTTCTCATCCGTCACTCACATCTGCCGA
+GACGTTAACTACGGATGAATTATTCGCTACCTCCATGCCAACGGAGCATC
+AATATTTTTTATCTGCCTCTTCATTCACGTAGGACGCGGCCTCTACTACG
+GCTCTTACACATTCCTAGAGACATGAAACATTGGAATCATCCTACTTTTC
+ACAGTTATAGCTACAGCATTCATGGGCTATGTCCTACCATGAGGCCAAAT
+ATCCTTTTGAGGAGCAACAGTCATCACGAACCTCCTATCAGCAATTCCCT
+ACATCGGTACTACCCTCGTCGAGTGAATCTGAGGTGGATTCTCAGTAGAC
+AAAGCCACCCTTACCCGATTTTTTGCTTTCCACTTCATCCTACCCTTCAT
+CATCACAGCCCTGGTAGTCGTACATTTACTATTTCTTCACGAAACAGGAT
+CTAATAACCCCTCAGGAATCCCATCCGATATGGACAAAATCCCATTCCAC
+CCATATTATACAATTAAAGACATCCTAGGACTCCTCCTCCTGATCTTGCT
+CCTACTAACTCTAGTATTATTCTCCCCCGACCTCCTAGGAGACCCAGACA
+ACTACACCCCAGCTAACCCTCTCAGCACTCCCCCTCATATTAAACCAGAA
+TGGTACTTCCTGTTTGCCTACGCCATCCTACGCTCCATTCCCAACAAACT
+AGGCGGCGTATTAGCCCTAATCCTCTCCATCCTGATCCTAGCACTCATCC
+CCACCCTCCACATATCAAAACAACGAAGCATAATATTCCGGCCTCTCAGC
+CAATGCGTATTCTGACTCTTAGTGGCAGACTTACTGACACTAACATGAAT
+CGGCGGACAGCCAGTGGAACACCCATACGTAATTATCGGCCAACTGGCCT
+CAATCCTCTACTTCTCCCTAATTCTCATTTTTATACCACTCGCAAGCACC
+ATCGAAAACAATCTTCTAAAATGAAGAGTCCCTGTAGTATATCGCACATT
+ACCCTGGTCTTGTAAACCAGAAAAGGGGGAAAACGTTTCCTCCCAAGGAC
+TATCAAGGAAGAAGCTCTAGCTCCACCATCAACACCCAAAGCTGAAATTC
+TACTTAAACTATTCCTTGATTTCTTCCCCTAAACGACAACAATTTACCCT
+CATGTGCTATGTCAGTATCAGATTATACCCCCACATAACACCATACCCAC
+CTGACATGCAATATCTTATGAATGGCCTATGTACGTCGTGCATTAAATTG
+TCTGCCCCATGAATAATAAGCATGTACATAATATCATTTATCTTACATAA
+GTACATTATATTATTGATCGTGCATACCCCATCCAAGTCAAATCATTTCC
+AGTCAACACGCATATCACAGCCCATGTTCCACGAGCTTAATCACCAAGCC
+GCGGGAAATCAGCAACCCTCCCAACTACGTGTCCCAATCCTCGCTCCGGG
+CCCATCCAAACGTGGGGGTTTCTACAATGAAACTATACCTGGCATCTGGT
+TCTTTCTTCAGGGCCATTCCCACCCAACCTCGCCCATTCTTTCCCCTTAA
+ATAAGACATCTCGATGGACTAATGACTAATCAGCCCATGCTCACACATAA
+CTGTGATTTCATGCATTTGGTATCTTTTTATATTTGGGGATGCTATGACT
+CAGCTATGGCCGTCAAAGGCCTCGACGCAGTCAATTAAATTGAAGCTGGA
+CTTAAATTGAACGTTATTCCTCCGCATCAGCAACCATAAGGTGTTATTCA
+GTCCATGGTAGCGGGACATAGGAAACAAgtgcacctgtgcacctgtgcac
+ctgtgcacctgtgcacctgtgcacctgtgcacctgtgcacctgtgcacct
+gtgcacctgtgcacctgtgcacctgtgcacctgtgcacctgtgcacctgt
+gcacctgtgcacctgtgcacctgtgcacctgtgcacctgtgcacctgtgc
+acctgtgcacctgtgcacctgtgcacctgtgcacctgtgcacctgtgcac
+ctgtgcacctACCCGCGCAGTAAGCAAGTAATATAGCTTTCTTAATCAAA
+CCCCCCCTACCCCCCATTAAACTCCACATATGTACATTCAACACAATCTT
+GCCAAACCCCAAAAACAAGACTAAACAATGCACAATACTTCATGAAGCTT
+AACCCTCGCATGCCAACCATAATAACTCAACACACCTAACAATCTTAACA
+GAACTTTCCCCCCGCCATTAATACCAACATGCTACTTTAATCAATAAAAT
+TTCCATAGACAGGCATCCCCCTAGATCTAATTTTCTAAATCTGTCAACCC
+TTCTTCCCCC
diff --git a/tool_collections/samtools/sam_to_bam/test-data/chr_m.fasta.fai b/tool_collections/samtools/sam_to_bam/test-data/chr_m.fasta.fai
new file mode 100644
index 00000000000..64c0d2c398a
--- /dev/null
+++ b/tool_collections/samtools/sam_to_bam/test-data/chr_m.fasta.fai
@@ -0,0 +1 @@
+chrM	16660	6	50	51
diff --git a/tool_collections/samtools/sam_to_bam/test-data/sam_to_bam_in1.sam b/tool_collections/samtools/sam_to_bam/test-data/sam_to_bam_in1.sam
new file mode 100644
index 00000000000..85ba34517bc
--- /dev/null
+++ b/tool_collections/samtools/sam_to_bam/test-data/sam_to_bam_in1.sam
@@ -0,0 +1,13 @@
+@HD	VN:1.0	SO:coordinate
+@SQ	SN:chrM	LN:100001
+@RG	ID:rg1	SM:s1
+HWI-EAS91_1_30788AAXX:1:1:1513:715	16	chrM	9563	25	36M	*	0	0	CTGACTACCACAACTAAACATCTATGCNNAAAAAAC	I+-II?IDIIIIIIIIIIIIIIIIIII""IIIIIII	NM:i:1	X1:i:1	MD:Z:7N0N27	RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1698:516	16	chrM	2735	25	36M	*	0	0	TTTACACTCAGAGGTTCAACTCCTCTCNNTAACAAC	I9IIIII5IIIIIIIIIIIIIIIIIII""IIIIIII	NM:i:1	X1:i:1	MD:Z:7N0N27	RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1491:637	16	chrM	10864	25	36M	*	0	0	TGTAGAAGCCCCAATTGCCGGATCCATNNTGCTAGC	DBAIIIIIIIIIIIFIIIIIIIIIIII""IIIIIII	NM:i:1	X1:i:1	MD:Z:7N0N27	RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1711:249	16	chrM	10617	25	36M	*	0	0	ACCAAACAGAACGCCTGAACGCAGGCCNNTACTTCC	IIIIIIIIIIIIIIIIIIIIIIIIIII""IIIIIII	NM:i:1	X1:i:1	MD:Z:7N0N27	RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1634:211	0	chrM	9350	25	36M	*	0	0	GAAGCAGNNGCTTGATACTGACACTTCGTCGACGTA	IIIIIII""IIIIIIIIIIIIIIIIIIIIII9IIDF	NM:i:1	X1:i:1	MD:Z:7N0N27	RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1218:141	16	chrM	14062	25	36M	*	0	0	ACAAAACTAACAACAAAAATAACACTCNNAATAAAC	I+IIII1IIIIIIIIIIIIIIIIIIII""IIIIIII	NM:i:1	X1:i:1	MD:Z:7N0N27	RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1398:854	16	chrM	3921	25	36M	*	0	0	CACCCTTCCCGTACTAATAAATCCCCTNNTCTTCAC	IIIII=AIIIIIIIIIIIIIIBIIIII""IIIIIII	NM:i:1	X1:i:1	MD:Z:7N0N27	RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1310:991	16	chrM	10002	25	36M	*	0	0	CTCCTATGCCTAGAAGGAATAATACTANNACTATTC	I:2IEI:IIDIIIIII4IIIIIIIIII""IIIIIII	NM:i:1	X1:i:1	MD:Z:7N0N27	RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1716:413	0	chrM	6040	25	36M	*	0	0	GATCCAANNCTTTATCAACACCTATTCTGATTCTTC	IIIIIII""IIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	X1:i:1	MD:Z:7N0N27	RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1630:59	16	chrM	12387	25	36M	*	0	0	TCATACTCGACCCCAACCTTACCAACCNNCCGCTCC	FIIHII;IIIIIIIIIIIIIIIIIIII""IIIIIII	NM:i:1	X1:i:1	MD:Z:7N0N27	RG:Z:rg1
diff --git a/tool_collections/samtools/sam_to_bam/test-data/sam_to_bam_noheader_in2.sam b/tool_collections/samtools/sam_to_bam/test-data/sam_to_bam_noheader_in2.sam
new file mode 100644
index 00000000000..87881711adc
--- /dev/null
+++ b/tool_collections/samtools/sam_to_bam/test-data/sam_to_bam_noheader_in2.sam
@@ -0,0 +1,10 @@
+HWI-EAS91_1_30788AAXX:1:1:1513:715	16	chrM	9563	25	36M	*	0	0	CTGACTACCACAACTAAACATCTATGCNNAAAAAAC	I+-II?IDIIIIIIIIIIIIIIIIIII""IIIIIII	NM:i:1	X1:i:1	MD:Z:7N0N27	RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1698:516	16	chrM	2735	25	36M	*	0	0	TTTACACTCAGAGGTTCAACTCCTCTCNNTAACAAC	I9IIIII5IIIIIIIIIIIIIIIIIII""IIIIIII	NM:i:1	X1:i:1	MD:Z:7N0N27	RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1491:637	16	chrM	10864	25	36M	*	0	0	TGTAGAAGCCCCAATTGCCGGATCCATNNTGCTAGC	DBAIIIIIIIIIIIFIIIIIIIIIIII""IIIIIII	NM:i:1	X1:i:1	MD:Z:7N0N27	RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1711:249	16	chrM	10617	25	36M	*	0	0	ACCAAACAGAACGCCTGAACGCAGGCCNNTACTTCC	IIIIIIIIIIIIIIIIIIIIIIIIIII""IIIIIII	NM:i:1	X1:i:1	MD:Z:7N0N27	RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1634:211	0	chrM	9350	25	36M	*	0	0	GAAGCAGNNGCTTGATACTGACACTTCGTCGACGTA	IIIIIII""IIIIIIIIIIIIIIIIIIIIII9IIDF	NM:i:1	X1:i:1	MD:Z:7N0N27	RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1218:141	16	chrM	14062	25	36M	*	0	0	ACAAAACTAACAACAAAAATAACACTCNNAATAAAC	I+IIII1IIIIIIIIIIIIIIIIIIII""IIIIIII	NM:i:1	X1:i:1	MD:Z:7N0N27	RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1398:854	16	chrM	3921	25	36M	*	0	0	CACCCTTCCCGTACTAATAAATCCCCTNNTCTTCAC	IIIII=AIIIIIIIIIIIIIIBIIIII""IIIIIII	NM:i:1	X1:i:1	MD:Z:7N0N27	RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1310:991	16	chrM	10002	25	36M	*	0	0	CTCCTATGCCTAGAAGGAATAATACTANNACTATTC	I:2IEI:IIDIIIIII4IIIIIIIIII""IIIIIII	NM:i:1	X1:i:1	MD:Z:7N0N27	RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1716:413	0	chrM	6040	25	36M	*	0	0	GATCCAANNCTTTATCAACACCTATTCTGATTCTTC	IIIIIII""IIIIIIIIIIIIIIIIIIIIIIIIIII	NM:i:1	X1:i:1	MD:Z:7N0N27	RG:Z:rg1
+HWI-EAS91_1_30788AAXX:1:1:1630:59	16	chrM	12387	25	36M	*	0	0	TCATACTCGACCCCAACCTTACCAACCNNCCGCTCC	FIIHII;IIIIIIIIIIIIIIIIIIII""IIIIIII	NM:i:1	X1:i:1	MD:Z:7N0N27	RG:Z:rg1
diff --git a/tool_collections/samtools/sam_to_bam/test-data/sam_to_bam_out1.bam b/tool_collections/samtools/sam_to_bam/test-data/sam_to_bam_out1.bam
new file mode 100644
index 0000000000000000000000000000000000000000..35992dfd291727ea4ff82bd2fb0a27ad06bf35dd
GIT binary patch
literal 610
zcmb2|=3rp}f&Xj_PR>jWxeUccUs6jF5)ukN6g=elyxHiA@dKW3+r7@6*Y`Sk##_hp
zX%PqC6P|CIz0PTTdisS?%YcE&S+8B8+|OTp^`a8za|!XQlbrS37D%pf;0ZduVMFgk
zMuzVzzNSS2Es;mF?K<PqE56wxh7#-_dX6jIIKsMF=~_zq%f$-DH!IJtoEetGAM47m
zSFKs`QKRD1ECZ)J+naUWb(`h3ec50>d*;oi{2h6}c$g)hO_2X@<9{w7UObwkx6NI}
z;kHJ}efzMh|E&KMeEBrBD!x;=aMBUiNG;RZo)_&ZLr?iU$#^g46lR?8>VQ!a!y?r*
z7L6^Y4ffg`Vcjo3))@%@KKSwAo*Bn3dp6$NJFoty%7vR=-@Y|Y*D2g&<ZUhaIN^ul
zMUmih{?ijYwtt<RzJJz@woRYiw;OHgTqCg4$Mw?5DT&tMVbW)IY+Il+<NoKL(QBvH
za_>#4oOG&FU~kTWdoj+=p3WV-y<A_VW?H|#Sg|(8?wi$FAC*l4k?PE>EryE>jdyj5
zNeJKA#s5LXt^Aeop4ZpYm8OMWO=pWSk6GQgDB|!rrWK8fZ31lt+0`$UfB(AU9mb)k
zo_X~o$6=p|Qg^IZELPq4NB-^C|C`S1?@7s2O_?V$cTHhsVPCH7u1Jg6YrEt&&VOFC
zXXcyVUf&GeI;!uad+KbqGji?>^gE`TsIrTz(fX9$fycXD%Vt))nh7pFy*A&t$#BPQ
k(c~^k7w%hp4!gf^oBxJQ?x8m$GaF{SN;5En6BUR60H{m~@&Et;

literal 0
HcmV?d00001

diff --git a/tool_collections/samtools/sam_to_bam/test-data/sam_to_bam_out2.bam b/tool_collections/samtools/sam_to_bam/test-data/sam_to_bam_out2.bam
new file mode 100644
index 0000000000000000000000000000000000000000..35992dfd291727ea4ff82bd2fb0a27ad06bf35dd
GIT binary patch
literal 610
zcmb2|=3rp}f&Xj_PR>jWxeUccUs6jF5)ukN6g=elyxHiA@dKW3+r7@6*Y`Sk##_hp
zX%PqC6P|CIz0PTTdisS?%YcE&S+8B8+|OTp^`a8za|!XQlbrS37D%pf;0ZduVMFgk
zMuzVzzNSS2Es;mF?K<PqE56wxh7#-_dX6jIIKsMF=~_zq%f$-DH!IJtoEetGAM47m
zSFKs`QKRD1ECZ)J+naUWb(`h3ec50>d*;oi{2h6}c$g)hO_2X@<9{w7UObwkx6NI}
z;kHJ}efzMh|E&KMeEBrBD!x;=aMBUiNG;RZo)_&ZLr?iU$#^g46lR?8>VQ!a!y?r*
z7L6^Y4ffg`Vcjo3))@%@KKSwAo*Bn3dp6$NJFoty%7vR=-@Y|Y*D2g&<ZUhaIN^ul
zMUmih{?ijYwtt<RzJJz@woRYiw;OHgTqCg4$Mw?5DT&tMVbW)IY+Il+<NoKL(QBvH
za_>#4oOG&FU~kTWdoj+=p3WV-y<A_VW?H|#Sg|(8?wi$FAC*l4k?PE>EryE>jdyj5
zNeJKA#s5LXt^Aeop4ZpYm8OMWO=pWSk6GQgDB|!rrWK8fZ31lt+0`$UfB(AU9mb)k
zo_X~o$6=p|Qg^IZELPq4NB-^C|C`S1?@7s2O_?V$cTHhsVPCH7u1Jg6YrEt&&VOFC
zXXcyVUf&GeI;!uad+KbqGji?>^gE`TsIrTz(fX9$fycXD%Vt))nh7pFy*A&t$#BPQ
k(c~^k7w%hp4!gf^oBxJQ?x8m$GaF{SN;5En6BUR60H{m~@&Et;

literal 0
HcmV?d00001

diff --git a/tool_collections/samtools/sam_to_bam/test-data/sam_to_bam_out3.bam b/tool_collections/samtools/sam_to_bam/test-data/sam_to_bam_out3.bam
new file mode 100644
index 0000000000000000000000000000000000000000..e47f62a31f871c5a3da3d1735165f55e8722cf84
GIT binary patch
literal 593
zcmb2|=3rp}f&Xj_PR>jWF$~2;UsA6mCnOYnD0s;8d9%?K<7YhIwtJmBukUs8jJJ;G
z(;^POCp_Oad!5tx^z;j(mVvRc@q;9mR|%|16EvDTl$n_sp5(V)b_bd%k7oFF#-&$$
zvqcOg*gy0fSGsY8b+gj7l=PR26^w6Io?kgLEQdeVm0zz~v*M#h#iv;YPI<OB>$>YU
z%WeCz!F=}2n@#yU@_z9!OFo+*|KG;{TtK{dG)HfnyNbhYjgtHJVORfI|0($LX=+t`
zr*Pq<Bd(EJrn5aS+Es?0@_CZ+Ud}1ZIN{X+qa=n!s%b15TTC15wK>ANUw*7J5dMAe
z<H0>Mj$QU_ytj8={ZEw(H@&`nYn-lAxXH-dTJmwi55<cj!RP#^CwOfCIyrs+tQ&2c
zKD%!>+S0j3V5g7krIS+<t;55l&+OQ?KxfAN&p)HrPOatMn^HOHRHwk+oCEh_oSi+L
zJ9vA!zDmusetofGZI0bHtFt~Tn*<`&nOR#57a1Dw>J*a@zOjq{gNR%CE8{({ucs?b
z3%#1o7GoZ>x^Yp&;d4wY8Wr0F+6uC(Unu|nb;mo5Ls32R>Pe2nJ`<(xSg%;Dy77<v
z+pqsOo!8%!lBt?9Ph{?z!pg$FT-jZb7O~fM$!(nfylBtNH^05U8M<{;-%0n>*=%Rz
z+!^S1OgB+w7gwY8DZK-ace|F&taddMTzYzKzHyV`j@zQiU6L-`xA+`(f8RF$4V&CU
SZ$@S|%y^Y%U<Ril5CH&$_5*?d

literal 0
HcmV?d00001

diff --git a/tool_collections/samtools/sam_to_bam/tool-data/fasta_indexes.loc.sample b/tool_collections/samtools/sam_to_bam/tool-data/fasta_indexes.loc.sample
new file mode 100644
index 00000000000..fd8fb70ac28
--- /dev/null
+++ b/tool_collections/samtools/sam_to_bam/tool-data/fasta_indexes.loc.sample
@@ -0,0 +1,29 @@
+#This is a sample file distributed with Galaxy that enables tools
+#to use a directory of Samtools indexed sequences data files.  You will need
+#to create these data files and then create a fasta_indexes.loc file
+#similar to this one (store it in this directory) that points to
+#the directories in which those files are stored. The fasta_indexes.loc
+#file has this format (white space characters are TAB characters):
+#
+# <unique_build_id>	<dbkey>	<display_name>	<file_base_path>
+#
+#So, for example, if you had hg19 Canonical indexed stored in
+#
+# /depot/data2/galaxy/hg19/sam/,
+#
+#then the fasta_indexes.loc entry would look like this:
+#
+#hg19canon	hg19	Human (Homo sapiens): hg19 Canonical	/depot/data2/galaxy/hg19/sam/hg19canon.fa
+#
+#and your /depot/data2/galaxy/hg19/sam/ directory
+#would contain hg19canon.fa and hg19canon.fa.fai files.
+#
+#Your fasta_indexes.loc file should include an entry per line for
+#each index set you have stored.  The file in the path does actually
+#exist, but it should never be directly used. Instead, the name serves
+#as a prefix for the index file.  For example:
+#
+#hg18canon	hg18	Human (Homo sapiens): hg18 Canonical	/depot/data2/galaxy/hg18/sam/hg18canon.fa
+#hg18full	hg18	Human (Homo sapiens): hg18 Full	/depot/data2/galaxy/hg18/sam/hg18full.fa
+#hg19canon	hg19	Human (Homo sapiens): hg19 Canonical	/depot/data2/galaxy/hg19/sam/hg19canon.fa
+#hg19full	hg19	Human (Homo sapiens): hg19 Full	/depot/data2/galaxy/hg19/sam/hg19full.fa
diff --git a/tool_collections/samtools/sam_to_bam/tool_data_table_conf.xml.sample b/tool_collections/samtools/sam_to_bam/tool_data_table_conf.xml.sample
new file mode 100644
index 00000000000..c34ade01890
--- /dev/null
+++ b/tool_collections/samtools/sam_to_bam/tool_data_table_conf.xml.sample
@@ -0,0 +1,7 @@
+<!-- Use the file tool_data_table_conf.xml.oldlocstyle if you don't want to update your loc files as changed in revision 4550:535d276c92bc-->
+<tables>
+    <table name="fasta_indexes" comment_char="#">
+        <columns>value, dbkey, name, path</columns>
+        <file path="tool-data/fasta_indexes.loc" />
+    </table>
+</tables>
diff --git a/tool_collections/samtools/sam_to_bam/tool_data_table_conf.xml.test b/tool_collections/samtools/sam_to_bam/tool_data_table_conf.xml.test
new file mode 100644
index 00000000000..a71a944e38c
--- /dev/null
+++ b/tool_collections/samtools/sam_to_bam/tool_data_table_conf.xml.test
@@ -0,0 +1,10 @@
+<tables>
+    <table name="fasta_indexes" comment_char="#">
+        <columns>value, dbkey, name, path</columns>
+        <file path="${__HERE__}/test-data/cached_locally/fasta_indexes.loc" />
+    </table>
+    <table name="all_fasta" comment_char="#">
+        <columns>value, dbkey, name, path</columns>
+        <file path="${__HERE__}/test-data/cached_locally/all_fasta.loc" />
+    </table>
+</tables>
\ No newline at end of file
diff --git a/tool_collections/samtools/sam_to_bam/tool_dependencies.xml b/tool_collections/samtools/sam_to_bam/tool_dependencies.xml
new file mode 120000
index 00000000000..b11b378d63c
--- /dev/null
+++ b/tool_collections/samtools/sam_to_bam/tool_dependencies.xml
@@ -0,0 +1 @@
+../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_bedcov/.shed.yml b/tool_collections/samtools/samtools_bedcov/.shed.yml
new file mode 100644
index 00000000000..cb1b2636a41
--- /dev/null
+++ b/tool_collections/samtools/samtools_bedcov/.shed.yml
@@ -0,0 +1,11 @@
+categories:
+- SAM
+- Sequence Analysis
+description: Calculate read depth on BAM files
+long_description: |
+  This tool uses the SAMtools toolkit to produce read depth per BED region.
+name: samtools_bedcov
+owner: devteam
+homepage_url: http://www.htslib.org/
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_bedcov
+type: unrestricted
diff --git a/tool_collections/samtools/samtools_bedcov/macros.xml b/tool_collections/samtools/samtools_bedcov/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/samtools/samtools_bedcov/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml b/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml
new file mode 100644
index 00000000000..db09bd0ebd6
--- /dev/null
+++ b/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml
@@ -0,0 +1,57 @@
+<tool id="samtools_bedcov" name="BedCov" version="2.0">
+  <description>calculate read depth for a set of genomic intervals</description>
+  <macros>
+    <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"></expand>
+    <expand macro="stdio"></expand>
+    <expand macro="version_command"></expand>
+    <command><![CDATA[
+        for bamfile in 
+        #for dataset in $input_bams:
+            "${dataset}"
+        #end for
+        ; do
+            if [ ! -f \$bamfile.bai ] ; then
+                ln -s \$bamfile && samtools index `basename \$bamfile` ;
+            else
+                ln -s \$bamfile && ln -s \$bamfile.bai ;
+            fi ;
+        done ;
+        samtools bedcov "${input_bed}"
+        #for dataset in $input_bams:
+            `basename "${dataset}"`
+        #end for
+        > "${output}"
+        ]]>
+    </command>
+    <inputs>
+        <param name="input_bed" type="data" format="bed" label="BED file" />
+        <param name="input_bams" type="data" format="bam" label="BAM file" multiple="true" />
+    </inputs>
+    <outputs>
+        <data format="tabular" name="output" label="${tool.name} on ${on_string}" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="input_bed" value="eboVir3.1.bed" ftype="bed" />
+            <param name="input_bams" value="eboVir3.bam" ftype="bam" />
+            <output name="output" file="samtools_bedcov_out1.tab" />
+        </test>
+        <test>
+            <param name="input_bed" value="eboVir3.1.bed" ftype="bed" />
+            <param name="input_bams" value="eboVir3.bam,eboVir3.2.bam" ftype="bam" />
+            <output name="output" file="samtools_bedcov_out2.tab" />
+        </test>
+    </tests>
+    <help>
+**What it does**
+
+Calculates read depth for regions listed in a BED dataset using ``samtools bedcov`` command::
+
+ samtools bedcov [INPUT BED] [INPUT BAM1] ... [INPUT BAMn] > [OUTPUT]
+
+    </help>
+    <expand macro="citations"></expand>
+</tool>
+
diff --git a/tool_collections/samtools/samtools_bedcov/test-data/eboVir3.1.bed b/tool_collections/samtools/samtools_bedcov/test-data/eboVir3.1.bed
new file mode 100644
index 00000000000..d25dd55f082
--- /dev/null
+++ b/tool_collections/samtools/samtools_bedcov/test-data/eboVir3.1.bed
@@ -0,0 +1,3 @@
+eboVir3	500	1500
+eboVir3	1500	2000
+eboVir3	1500	3000
diff --git a/tool_collections/samtools/samtools_bedcov/test-data/eboVir3.2.bam b/tool_collections/samtools/samtools_bedcov/test-data/eboVir3.2.bam
new file mode 100644
index 0000000000000000000000000000000000000000..2fdd7354b8b4a1202d17ec54d375b45025c66bc5
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diff --git a/tool_collections/samtools/samtools_bedcov/test-data/eboVir3.2.bed b/tool_collections/samtools/samtools_bedcov/test-data/eboVir3.2.bed
new file mode 100644
index 00000000000..d25dd55f082
--- /dev/null
+++ b/tool_collections/samtools/samtools_bedcov/test-data/eboVir3.2.bed
@@ -0,0 +1,3 @@
+eboVir3	500	1500
+eboVir3	1500	2000
+eboVir3	1500	3000
diff --git a/tool_collections/samtools/samtools_bedcov/test-data/eboVir3.bam b/tool_collections/samtools/samtools_bedcov/test-data/eboVir3.bam
new file mode 100644
index 0000000000000000000000000000000000000000..c0e64957d9229a43c6ac16640951bdceff05ed81
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diff --git a/tool_collections/samtools/samtools_bedcov/test-data/samtools_bedcov_out1.tab b/tool_collections/samtools/samtools_bedcov/test-data/samtools_bedcov_out1.tab
new file mode 100644
index 00000000000..ce32b596e0b
--- /dev/null
+++ b/tool_collections/samtools/samtools_bedcov/test-data/samtools_bedcov_out1.tab
@@ -0,0 +1,3 @@
+eboVir3	500	1500	7919
+eboVir3	1500	2000	3009
+eboVir3	1500	3000	9986
diff --git a/tool_collections/samtools/samtools_bedcov/test-data/samtools_bedcov_out2.tab b/tool_collections/samtools/samtools_bedcov/test-data/samtools_bedcov_out2.tab
new file mode 100644
index 00000000000..4a12f060dfe
--- /dev/null
+++ b/tool_collections/samtools/samtools_bedcov/test-data/samtools_bedcov_out2.tab
@@ -0,0 +1,3 @@
+eboVir3	500	1500	7919	2094
+eboVir3	1500	2000	3009	1064
+eboVir3	1500	3000	9986	3455
diff --git a/tool_collections/samtools/samtools_bedcov/tool_dependencies.xml b/tool_collections/samtools/samtools_bedcov/tool_dependencies.xml
new file mode 120000
index 00000000000..b11b378d63c
--- /dev/null
+++ b/tool_collections/samtools/samtools_bedcov/tool_dependencies.xml
@@ -0,0 +1 @@
+../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_calmd/.shed.yml b/tool_collections/samtools/samtools_calmd/.shed.yml
new file mode 100644
index 00000000000..73d004debcf
--- /dev/null
+++ b/tool_collections/samtools/samtools_calmd/.shed.yml
@@ -0,0 +1,10 @@
+categories:
+- SAM
+description: recalculate MD/NM tags
+long_description: |
+  Generates the MD tag using ``samtools calmd`` command.
+name: samtools_calmd
+owner: devteam
+homepage_url: http://www.htslib.org/
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_calmd
+type: unrestricted
diff --git a/tool_collections/samtools/samtools_calmd/macros.xml b/tool_collections/samtools/samtools_calmd/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/samtools/samtools_calmd/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_calmd/samtools_calmd.xml b/tool_collections/samtools/samtools_calmd/samtools_calmd.xml
new file mode 100644
index 00000000000..e0c7d0243c1
--- /dev/null
+++ b/tool_collections/samtools/samtools_calmd/samtools_calmd.xml
@@ -0,0 +1,99 @@
+<tool id="samtools_calmd" name="CalMD" version="2.0">
+  <description>recalculate MD/NM tags</description>
+  <macros>
+    <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"></expand>
+  <expand macro="stdio"></expand>
+  <expand macro="version_command"></expand>
+    <command><![CDATA[
+        #if str( $reference_source.reference_source_selector ) == "history":
+            #set ref_fa = 'ref.fa'
+            ln -s "${reference_source.ref_fasta}" ref.fa && samtools faidx ref.fa &&
+        #else:
+            #set ref_fa = str( $reference_source.ref_fasta.fields.path )
+        #end if
+        samtools calmd
+        #if str($option_set.option_sets) == 'advanced':
+            $option_set.change_identical $option_set.modify_quality $option_set.compute_cap $option_set.extended_baq
+        #end if
+        -b "$input_bam" "$ref_fa" > "$calmd_output" ]]>
+    </command>
+    <inputs>
+        <param format="bam" name="input_bam" type="data" label="BAM file to recalculate" />
+        <conditional name="reference_source">
+            <param name="reference_source_selector" type="select" label="Choose the source for the reference genome">
+                <option value="cached">Use a built-in genome</option>
+                <option value="history">Use a genome from the history</option>
+            </param>
+            <when value="cached">
+                <param name="ref_fasta" type="select" label="Using reference genome">
+                    <options from_data_table="fasta_indexes">
+                        <filter type="data_meta" column="0" key="dbkey" ref="input_bam" />
+                        <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file" />
+                    </options>
+                </param>
+            </when>
+            <when value="history">
+                <param name="ref_fasta" type="data" format="fasta" label="Using reference file" />
+            </when>
+        </conditional>
+        <conditional name="option_set">
+            <param name="option_sets" type="select" label="Options">
+                <option value="default">Use defaults</option>
+                <option value="advanced">Advanced options</option>
+            </param>
+            <when value="default" />
+            <when value="advanced">
+                <param name="change_identical" type="boolean" truevalue="-e" falsevalue="" checked="False" label="Change identical bases to '='" help="-e"/>
+                <param name="modify_quality" type="boolean" truevalue="-A" falsevalue="" checked="False" label="Modify the quality string" help="-A"/>
+                <param name="compute_cap" type="boolean" truevalue="-r" falsevalue="" checked="False" label="Compute BQ or cap baseQ by BAQ" help="-r"/>
+                <param name="extended_baq" type="boolean" truevalue="-E" falsevalue="" checked="False" label="Extended BAQ for better sensitivity" help="-E"/>
+            </when>
+        </conditional>
+    </inputs>
+    <outputs>
+        <data format="bam" name="calmd_output" label="${tool.name} on ${on_string}" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="option_sets" value="default" /> 
+            <param name="input_bam" value="phiX.bam"/>
+            <param name="reference_source_selector" value="history" />
+            <param name="ref_fasta" value="phiX.fasta" />
+            <output name="calmd_output" file="samtools_calmd_out_1.bam" ftype="bam" />
+        </test>
+        <test>
+            <param name="option_sets" value="advanced" /> 
+            <param name="change_identical" value="true" />
+            <param name="extended_baq" value="true" />
+            <param name="input_bam" value="phiX.bam"/>
+            <param name="reference_source_selector" value="history" />
+            <param name="ref_fasta" value="phiX.fasta" />
+            <output name="calmd_output" file="samtools_calmd_out_2.bam" ftype="bam" />
+        </test>
+    </tests>
+    <help>
+**What it does**
+
+Generates the MD tag using ``samtools calmd`` command. If the MD tag (see SAM format refernce below for explanation of SAM/BAM tags) is already present, this command will give a warning if the MD tag generated is different from the existing tag. Outputs a BAM file. The command has the following options::
+
+  -e       change identical bases to '='
+  -A       modify the quality string
+  -r       compute the BQ tag (without -A) or cap baseQ by BAQ (with -A)
+  -E       extended BAQ for better sensitivity but lower specificity
+  
+-----
+
+**NM and MD tags**
+
+From SAM format specification::
+
+  MD (string)  String for mismatching positions. Regex : [0-9]+(([A-Z]|\^[A-Z]+)[0-9]+)*7
+  NM (indeger) Edit distance to the reference, including ambiguous bases but excluding clipping
+  
+See refernces for more information about SAM format tags.
+
+    </help>
+    <expand macro="citations"></expand>
+</tool>
diff --git a/tool_collections/samtools/samtools_calmd/test-data/phiX.bam b/tool_collections/samtools/samtools_calmd/test-data/phiX.bam
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a00000{{{d;LjnLB00RI3000000001Wf+)KH

literal 0
HcmV?d00001

diff --git a/tool_collections/samtools/samtools_calmd/test-data/phiX.fasta b/tool_collections/samtools/samtools_calmd/test-data/phiX.fasta
new file mode 100644
index 00000000000..53df885dc48
--- /dev/null
+++ b/tool_collections/samtools/samtools_calmd/test-data/phiX.fasta
@@ -0,0 +1,79 @@
+>phiX174
+GAGTTTTATCGCTTCCATGACGCAGAAGTTAACACTTTCGGATATTTCTGATGAGTCGAAAAATTATCTT
+GATAAAGCAGGAATTACTACTGCTTGTTTACGAATTAAATCGAAGTGGACTGCTGGCGGAAAATGAGAAA
+ATTCGACCTATCCTTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATCAACTAACGATTCTG
+TCAAAAACTGACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGTTTA
+GATATGAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTGGATTACTATC
+TGAGTCCGATGCTGTTCAACCACTAATAGGTAAGAAATCATGAGTCAAGTTACTGAACAATCCGTACGTT
+TCCAGACCGCTTTGGCCTCTATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTAACCGAAGATGATTT
+CGATTTTCTGACGAGTAACAAAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCT
+TGCGTTTATGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTTTATTGCTGCCG
+TCATTGCTTATTATGTTCATCCCGTCAACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTAC
+GGAAAACATTATTAATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGTA
+CGCGCAGGAAACACTGACGTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAAATTACGTGCAGAAGGAG
+TGATGTAATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACT
+AAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGC
+CCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCA
+TCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATTTCAACTACTCCGGTTATCGCTGGCGAC
+TCCTTCGAGATGGACGCCGTTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTA
+CTGTAGACATTTTTACTTTTTATGTCCCTCATCGTCACGTTTATGGTGAACAGTGGATTAAGTTCATGAA
+GGATGGTGTTAATGCCACTCCTCTCCCGACTGTTAACACTACTGGTTATATTGACCATGCCGCTTTTCTT
+GGCACGATTAACCCTGATACCAATAAAATCCCTAAGCATTTGTTTCAGGGTTATTTGAATATCTATAACA
+ACTATTTTAAAGCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATCAAGATGATGC
+TCGTTATGGTTTCCGTTGCTGCCATCTCAAAAACATTTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTT
+TCTCGCCAAATGACGACTTCTACCACATCTATTGACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGC
+ATACTGACCAAGAACGTGATTACTTCATGCAGCGTTACCGTGATGTTATTTCTTCATTTGGAGGTAAAAC
+CTCTTATGACGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCTATGATGTT
+GATGGAACTGACCAAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAACATTCTGTGC
+CGCGTTTCTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGCGAC
+TAAAGAGATTCAGTACCTTAACGCTAAAGGTGCTTTGACTTATACCGATATTGCTGGCGACCCTGTTTTG
+TATGGCAACTTGCCGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGATTCGTCTAAGAAGT
+TTAAGATTGCTGAGGGTCAGTGGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCTTCTTGA
+AGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGAT
+TATGACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGAATAGTCAGGTTAAATTTAATGTGACCGTTT
+ATCGCAATCTGCCGACCACTCGCGATTCAATCATGACTTCGTGATAAAAGATTGAGTGTGAGGTTATAAC
+GCCGAAGCGGTAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACCAAGCGAAGCGCGGTAGGTTTTCTGC
+TTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTCGCCATAATTCAAACTTTTTTTCTGATAAGCTGGT
+TCTCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTTTACAGACACCTAAAGCTACATCGTCAACGTTA
+TATTTTGATAGTTTGACGGTTAATGCTGGTAATGGTGGTTTTCTTCATTGCATTCAGATGGATACATCTG
+TCAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGCCGACCCTAAATTTTTTGC
+CTGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTCCCGACTGCCTATGATGTTTATCCTTTG
+AATGGTCGCCATGATGGTGGTTATTATACCGTCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTACGC
+CGGGCAATAATGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTACTAAATGCCGCGGATTGGT
+TTCGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGGTGATTTATGTTTGGTG
+CTATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAA
+AGCCGCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACCGATAACAATACTGTAGGCATGGGTGATGCT
+GGTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCCCCTAGTTTTGTTTCTG
+GTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGGCACTTCTGCCGTTTCTGA
+TAAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGATTAT
+CTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGG
+TTGACGCCGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCAACTGGACAATCAGAAAGAGATTGCCGA
+GATGCAAAATGAGACTCAAAAAGAGATTGCTGGCATTCAGTCGGCGACTTCACGCCAGAATACGAAAGAC
+CAGGTATATGCACAAAATGAGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGCGTTGCGTCTATTA
+TGGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAAGCTCA
+AACGGCTGGTCAGTATTTTACCAATGACCAAATCAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGAC
+TTAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCTCTTCTCATATTGGCGCTACTGCAAAGGATATTT
+CTAATGTCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTTTTCATGGTATTGATAAAGCTGTTGCCGA
+TACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTCTAATTTGTCTAGGAAATAACCG
+TCAGGATTGACACCCTCCCAATTGTATGTTTTCATGCCTCCAAATCTTGGAGGCTTTTTTATGGTTCGTT
+CTTATTACCCTTCTGAATGTCACGCTGATTATTTTGACTTTGAGCGTATCGAGGCTCTTAAACCTGCTAT
+TGAGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAATGCTTGGCTTCCATAAGCAGATGGATAACCGC
+ATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAGGGCGTTGAGTTCGATAATGGTGATATGTATG
+TTGACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTACTGAGAAGTTAATGGATGA
+ATTGGCACAATGCTACAATGTGCTCCCCCAACTTGATATTAATAACACTATAGACCACCGCCCCGAAGGG
+GACGAAAAATGGTTTTTAGAGAACGAGAAGACGGTTACGCAGTTTTGCCGCAAGCTGGCTGCTGAACGCC
+CTCTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCAAGATT
+GCTGGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTACTATTCAGCGTTTGATGAATGCAATGCGACAG
+GCTCATGCTGATGGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCTGACGACCGATTAGAGGCGTTTT
+ATGATAATCCCAATGCTTTGCGTGACTATTTTCGTGATATTGGTCGTATGGTTCTTGCTGCCGAGGGTCG
+CAAGGCTAATGATTCACACGCCGACTGCTATCAGTATTTTTGTGTGCCTGAGTATGGTACAGCTAATGGC
+CGTCTTCATTTCCATGCGGTGCATTTTATGCGGACACTTCCTACAGGTAGCGTTGACCCTAATTTTGGTC
+GTCGGGTACGCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGTGGCCTTATGGTTACAGTATGCCCAT
+CGCAGTTCGCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAG
+CCGCTTAAAGCTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTAAATACGTTAACAAAAAGTCAGATA
+TGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCACTAAAAACCAAGCTGTCGCTACT
+TCCCAAGAAGCTGTTCAGAATCAGAATGAGCCGCAACTTCGGGATGAAAATGCTCACAATGACAAATCTG
+TCCACGGAGTGCTTAATCCAACTTACCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGATATTGAAGC
+AGAACGCAAAAAGAGAGATGAGATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTTTGGCGGCGCAACC
+TGTGACGACAAATCTGCTCAAATTTATGCGCGCTTCGATAAAAATGATTGGCGTATCCAACCTGCA
+
diff --git a/tool_collections/samtools/samtools_calmd/test-data/samtools_calmd_out_1.bam b/tool_collections/samtools/samtools_calmd/test-data/samtools_calmd_out_1.bam
new file mode 100644
index 0000000000000000000000000000000000000000..8a59449a5d17c642ff8a872042d6a8ceecfdb4ef
GIT binary patch
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diff --git a/tool_collections/samtools/samtools_calmd/test-data/samtools_calmd_out_2.bam b/tool_collections/samtools/samtools_calmd/test-data/samtools_calmd_out_2.bam
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diff --git a/tool_collections/samtools/samtools_calmd/tool-data/fasta_indexes.loc.sample b/tool_collections/samtools/samtools_calmd/tool-data/fasta_indexes.loc.sample
new file mode 100644
index 00000000000..fd8fb70ac28
--- /dev/null
+++ b/tool_collections/samtools/samtools_calmd/tool-data/fasta_indexes.loc.sample
@@ -0,0 +1,29 @@
+#This is a sample file distributed with Galaxy that enables tools
+#to use a directory of Samtools indexed sequences data files.  You will need
+#to create these data files and then create a fasta_indexes.loc file
+#similar to this one (store it in this directory) that points to
+#the directories in which those files are stored. The fasta_indexes.loc
+#file has this format (white space characters are TAB characters):
+#
+# <unique_build_id>	<dbkey>	<display_name>	<file_base_path>
+#
+#So, for example, if you had hg19 Canonical indexed stored in
+#
+# /depot/data2/galaxy/hg19/sam/,
+#
+#then the fasta_indexes.loc entry would look like this:
+#
+#hg19canon	hg19	Human (Homo sapiens): hg19 Canonical	/depot/data2/galaxy/hg19/sam/hg19canon.fa
+#
+#and your /depot/data2/galaxy/hg19/sam/ directory
+#would contain hg19canon.fa and hg19canon.fa.fai files.
+#
+#Your fasta_indexes.loc file should include an entry per line for
+#each index set you have stored.  The file in the path does actually
+#exist, but it should never be directly used. Instead, the name serves
+#as a prefix for the index file.  For example:
+#
+#hg18canon	hg18	Human (Homo sapiens): hg18 Canonical	/depot/data2/galaxy/hg18/sam/hg18canon.fa
+#hg18full	hg18	Human (Homo sapiens): hg18 Full	/depot/data2/galaxy/hg18/sam/hg18full.fa
+#hg19canon	hg19	Human (Homo sapiens): hg19 Canonical	/depot/data2/galaxy/hg19/sam/hg19canon.fa
+#hg19full	hg19	Human (Homo sapiens): hg19 Full	/depot/data2/galaxy/hg19/sam/hg19full.fa
diff --git a/tool_collections/samtools/samtools_calmd/tool_data_table_conf.xml.sample b/tool_collections/samtools/samtools_calmd/tool_data_table_conf.xml.sample
new file mode 100644
index 00000000000..a1c5e1ad88e
--- /dev/null
+++ b/tool_collections/samtools/samtools_calmd/tool_data_table_conf.xml.sample
@@ -0,0 +1,7 @@
+<?xml version="1.0" encoding="utf-8"?>
+<tables>
+    <table comment_char="#" name="fasta_indexes">
+        <columns>value, dbkey, name, path</columns>
+        <file path="tool-data/fasta_indexes.loc" />
+    </table>
+</tables>
diff --git a/tool_collections/samtools/samtools_calmd/tool_dependencies.xml b/tool_collections/samtools/samtools_calmd/tool_dependencies.xml
new file mode 120000
index 00000000000..b11b378d63c
--- /dev/null
+++ b/tool_collections/samtools/samtools_calmd/tool_dependencies.xml
@@ -0,0 +1 @@
+../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_flagstat/.shed.yml b/tool_collections/samtools/samtools_flagstat/.shed.yml
new file mode 100644
index 00000000000..9e42571d998
--- /dev/null
+++ b/tool_collections/samtools/samtools_flagstat/.shed.yml
@@ -0,0 +1,11 @@
+categories:
+- SAM
+- Statistics
+description: Provides simple stats on BAM files.
+long_description: |
+  This tool uses the SAMtools toolkit to produce simple stats on a BAM file.
+name: samtools_flagstat
+owner: devteam
+homepage_url: http://www.htslib.org/
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_flagstat
+type: unrestricted
diff --git a/tool_collections/samtools/samtools_flagstat/macros.xml b/tool_collections/samtools/samtools_flagstat/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/samtools/samtools_flagstat/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_flagstat/samtools_flagstat.xml b/tool_collections/samtools/samtools_flagstat/samtools_flagstat.xml
new file mode 100644
index 00000000000..1d53cec9a52
--- /dev/null
+++ b/tool_collections/samtools/samtools_flagstat/samtools_flagstat.xml
@@ -0,0 +1,45 @@
+<tool id="samtools_flagstat" name="Flagstat" version="2.0">
+  <description>tabulate descriptive stats for BAM datset</description>
+  <macros>
+    <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"></expand>
+  <expand macro="stdio"></expand>
+  <expand macro="version_command"></expand>
+  <command>samtools flagstat "$input1" > "$output1"
+  </command>
+  <inputs>
+    <param name="input1" type="data" format="bam" label="BAM File to Convert" />
+  </inputs>
+  <outputs>
+    <data name="output1" format="txt" />
+  </outputs>
+  <tests>
+    <test>
+      <param name="input1" value="samtools_flagstat_input1.bam" ftype="bam" />
+      <output name="output1" file="samtools_flagstat_out1.txt" />
+    </test>
+  </tests>
+  <help>
+
+**What it does**
+
+Uses ``samtools flagstat`` command to print descriptive information for a BAM dataset. Here is an example of such information::
+
+  200 + 0 in total (QC-passed reads + QC-failed reads)  
+  0 + 0 secondary
+  0 + 0 supplementary
+  0 + 0 duplicates
+  25 + 0 mapped (12.50%:nan%)
+  200 + 0 paired in sequencing
+  100 + 0 read1
+  100 + 0 read2
+  0 + 0 properly paired (0.00%:nan%)
+  0 + 0 with itself and mate mapped
+  25 + 0 singletons (12.50%:nan%)
+  0 + 0 with mate mapped to a different chr
+  0 + 0 with mate mapped to a different chr (mapQ>=5)
+  
+  </help>
+  <expand macro="citations"></expand>
+</tool>
diff --git a/tool_collections/samtools/samtools_flagstat/test-data/samtools_flagstat_input1.bam b/tool_collections/samtools/samtools_flagstat/test-data/samtools_flagstat_input1.bam
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diff --git a/tool_collections/samtools/samtools_flagstat/test-data/samtools_flagstat_out1.txt b/tool_collections/samtools/samtools_flagstat/test-data/samtools_flagstat_out1.txt
new file mode 100644
index 00000000000..086fe6c2dd3
--- /dev/null
+++ b/tool_collections/samtools/samtools_flagstat/test-data/samtools_flagstat_out1.txt
@@ -0,0 +1,13 @@
+200 + 0 in total (QC-passed reads + QC-failed reads)
+0 + 0 secondary
+0 + 0 supplementary
+0 + 0 duplicates
+25 + 0 mapped (12.50%:nan%)
+200 + 0 paired in sequencing
+100 + 0 read1
+100 + 0 read2
+0 + 0 properly paired (0.00%:nan%)
+0 + 0 with itself and mate mapped
+25 + 0 singletons (12.50%:nan%)
+0 + 0 with mate mapped to a different chr
+0 + 0 with mate mapped to a different chr (mapQ>=5)
diff --git a/tool_collections/samtools/samtools_flagstat/tool_dependencies.xml b/tool_collections/samtools/samtools_flagstat/tool_dependencies.xml
new file mode 120000
index 00000000000..b11b378d63c
--- /dev/null
+++ b/tool_collections/samtools/samtools_flagstat/tool_dependencies.xml
@@ -0,0 +1 @@
+../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_idxstats/.shed.yml b/tool_collections/samtools/samtools_idxstats/.shed.yml
new file mode 100644
index 00000000000..640eecf9ffd
--- /dev/null
+++ b/tool_collections/samtools/samtools_idxstats/.shed.yml
@@ -0,0 +1,12 @@
+categories:
+- SAM
+- Statistics
+description: BAM mapping statistics (using samtools idxstats)
+long_description: |
+  Runs "samtools idxstats" to get mapping statistics from a sorted
+  index BAM file as a tabular output file.
+name: samtools_idxstats
+owner: devteam
+homepage_url: http://www.htslib.org/
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_idxstats
+type: unrestricted
diff --git a/tool_collections/samtools/samtools_idxstats/macros.xml b/tool_collections/samtools/samtools_idxstats/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/samtools/samtools_idxstats/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_idxstats/samtools_idxstats.xml b/tool_collections/samtools/samtools_idxstats/samtools_idxstats.xml
new file mode 100644
index 00000000000..57bdb584a2d
--- /dev/null
+++ b/tool_collections/samtools/samtools_idxstats/samtools_idxstats.xml
@@ -0,0 +1,72 @@
+<tool id="samtools_idxstats" name="IdxStats" version="2.0">
+    <description>tabulate mapping statistics for BAM dataset</description>
+    <macros>
+      <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"></expand>
+    <expand macro="stdio"></expand>
+    <expand macro="version_command"></expand>
+    <command><![CDATA[
+if [ ! -f "${input}.bai" ] ; then
+    ln -s "${input}" input.bam ;
+    samtools sort -o sorted.bam -O bam -T sts "${input}" ;
+    samtools index sorted.bam ;
+    samtools idxstats sorted.bam > "${output}" ;
+else
+    samtools idxstats "${input}" > "${output}" ;
+fi]]>
+    </command>
+    <inputs>
+        <param name="input" type="data" format="bam" label="BAM file" />
+    </inputs>
+    <outputs>
+        <data format="tabular" name="output" label="${tool.name} on ${on_string}" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="input" value="phiX.bam" ftype="bam" />
+            <output name="output" file="samtools_idxstats_out.tabular" compare="diff" ftype="tabular" />
+        </test>
+    </tests>
+    <help>
+<![CDATA[
+**What it does**
+
+Runs the ``samtools idxstats`` command. It retrieves and prints stats in the index file.
+
+Input is a sorted and indexed BAM file, the output is tabular with
+four columns (one row per reference sequence plus a final line for
+unmapped reads)::
+
+ Column Description
+ ------ -----------------------------
+      1 Reference sequence identifier
+      2 Reference sequence length
+      3 Number of mapped reads
+      4 Number of placed but unmapped reads
+           (typically unmapped partners of mapped reads)
+
+------
+
+**Example** output from a *de novo* assembly::
+
+ contig_1   170035  98397     0
+ contig_2   403835 199564     0
+ contig_3   553102 288189     0
+ ...           ...    ...   ...
+ contig_603    653     50     0
+ contig_604    214      6     0
+ \*              0      0 50320
+
+
+In this example there were 604 contigs, each with one line in the output table,
+plus the final row (labelled with an asterisk) representing 50320 unmapped reads.
+In this BAM file, the final column was otherwise zero.
+
+------
+
+Peter J.A. Cock (2013), `Galaxy wrapper <https://github.com/peterjc/pico_galaxy/tree/master/tools/samtools_idxstats>`_ for the samtools idxstats command
+]]>
+    </help>
+    <expand macro="citations"></expand>
+</tool>
diff --git a/tool_collections/samtools/samtools_idxstats/test-data/phiX.bam b/tool_collections/samtools/samtools_idxstats/test-data/phiX.bam
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diff --git a/tool_collections/samtools/samtools_idxstats/test-data/samtools_idxstats_out.tabular b/tool_collections/samtools/samtools_idxstats/test-data/samtools_idxstats_out.tabular
new file mode 100644
index 00000000000..f51b037ac21
--- /dev/null
+++ b/tool_collections/samtools/samtools_idxstats/test-data/samtools_idxstats_out.tabular
@@ -0,0 +1,2 @@
+phiX174	5386	972	18
+*	0	0	10
diff --git a/tool_collections/samtools/samtools_idxstats/tool_dependencies.xml b/tool_collections/samtools/samtools_idxstats/tool_dependencies.xml
new file mode 120000
index 00000000000..b11b378d63c
--- /dev/null
+++ b/tool_collections/samtools/samtools_idxstats/tool_dependencies.xml
@@ -0,0 +1 @@
+../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_mpileup/.shed.yml b/tool_collections/samtools/samtools_mpileup/.shed.yml
new file mode 100644
index 00000000000..19d7b16c98c
--- /dev/null
+++ b/tool_collections/samtools/samtools_mpileup/.shed.yml
@@ -0,0 +1,13 @@
+categories:
+- SAM
+- Variant Analysis
+description: MPileup SNP and indel caller
+long_description: |
+  Generate BCF or pileup for one or multiple BAM files. Alignment
+  records are grouped by sample identifiers in @RG header lines. If sample identifiers
+  are absent, each input file is regarded as one sample.
+name: samtools_mpileup
+owner: devteam
+homepage_url: http://www.htslib.org/
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_mpileup
+type: unrestricted
diff --git a/tool_collections/samtools/samtools_mpileup/macros.xml b/tool_collections/samtools/samtools_mpileup/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/samtools/samtools_mpileup/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_mpileup/samtools_mpileup.xml b/tool_collections/samtools/samtools_mpileup/samtools_mpileup.xml
new file mode 100644
index 00000000000..35fecf8cf42
--- /dev/null
+++ b/tool_collections/samtools/samtools_mpileup/samtools_mpileup.xml
@@ -0,0 +1,372 @@
+<tool id="samtools_mpileup" name="MPileup" version="2.1.2">
+    <description>call variants</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements" />
+    <expand macro="stdio" />
+    <expand macro="version_command" />
+    <command>
+    <![CDATA[
+    #for $bam_count, $input_bam in enumerate( $reference_source.input_bam ):
+        ln -s "${input_bam}" "localbam_${bam_count}.bam" &&
+        ln -s "${input_bam.metadata.bam_index}" "localbam_${bam_count}.bam.bai" &&
+    #end for
+    #if $reference_source.reference_source_selector == "history":
+       ln -s "${reference_source.ref_file}" && samtools faidx `basename "${reference_source.ref_file}"` && samtools mpileup
+    #else:
+        samtools mpileup
+    #end if
+    #if $reference_source.reference_source_selector != "history":
+        -f "${reference_source.ref_file.fields.path}"
+    #else:
+        -f "${reference_source.ref_file}"
+    #end if
+    #for $bam_count, $input_bam in enumerate( $reference_source.input_bam ):
+        "localbam_${bam_count}.bam"
+    #end for
+    #if str( $advanced_options.advanced_options_selector ) == "advanced":
+        #if str( $advanced_options.filter_by_flags.filter_flags ) == "filter":
+            #if $advanced_options.filter_by_flags.require_flags:
+                --rf ${sum([int(flag) for flag in str($advanced_options.filter_by_flags.require_flags).split(',')])}
+            #end if
+            #if $advanced_options.filter_by_flags.exclude_flags:
+                --ff ${sum([int(flag) for flag in str($advanced_options.filter_by_flags.exclude_flags).split(',')])}
+            #end if
+        #end if
+        #if str( $advanced_options.limit_by_region.limit_by_regions ) == "paste":
+            -l "$pasted_regions"
+        #elif str( $advanced_options.limit_by_region.limit_by_regions ) == "history"
+            -l "$advanced_options.limit_by_region.bed_regions"
+        #end if
+        #if str( $advanced_options.exclude_read_group.exclude_read_groups ) == "paste":
+            -G "$excluded_read_groups"
+        #elif str( $advanced_options.exclude_read_group.exclude_read_groups ) == "history"
+            -G "$advanced_options.exclude_read_group.read_groups"
+        #end if
+        ${advanced_options.skip_anomalous_read_pairs}
+        ${advanced_options.disable_probabilistic_realignment}
+        -C "${advanced_options.coefficient_for_downgrading}"
+        -d "${advanced_options.max_reads_per_bam}"
+        ${advanced_options.extended_BAQ_computation}
+        -q "${advanced_options.minimum_mapping_quality}"
+        -Q "${advanced_options.minimum_base_quality}"
+        #if str( $advanced_options.region_string ):
+            -r "${advanced_options.region_string}"
+        #end if
+
+    #end if
+    #if str( $genotype_likelihood_computation_type.genotype_likelihood_computation_type_selector ) == 'perform_genotype_likelihood_computation':
+        ##
+
+        ${genotype_likelihood_computation_type.output_format}
+        ${genotype_likelihood_computation_type.compressed}
+
+        #if str( $genotype_likelihood_computation_type.output_tags ) != "None":
+            --output-tags "${genotype_likelihood_computation_type.output_tags}"
+        #end if
+
+        #if str( $genotype_likelihood_computation_type.perform_indel_calling.perform_indel_calling_selector ) == 'perform_indel_calling':
+            -o "${genotype_likelihood_computation_type.perform_indel_calling.gap_open_sequencing_error_probability}"
+            -e "${genotype_likelihood_computation_type.perform_indel_calling.gap_extension_sequencing_error_probability}"
+            -h "${genotype_likelihood_computation_type.perform_indel_calling.coefficient_for_modeling_homopolymer_errors}"
+            -L "${genotype_likelihood_computation_type.perform_indel_calling.skip_indel_calling_above_sample_depth}"
+            -m "${genotype_likelihood_computation_type.perform_indel_calling.minimum_gapped_reads_for_indel_candidates}"
+            --open-prob "${genotype_likelihood_computation_type.perform_indel_calling.open_seq_error_probability}"
+            -F "${genotype_likelihood_computation_type.perform_indel_calling.minimum_gapped_read_fraction}"
+            ${genotype_likelihood_computation_type.perform_indel_calling.gapped_read_per_sample}
+            #if len( $genotype_likelihood_computation_type.perform_indel_calling.platform_list_repeat ):
+                -P "${ ",".join( [ str( platform.platform_entry ) for platform in $genotype_likelihood_computation_type.perform_indel_calling.platform_list_repeat ] ) }"
+            #end if
+        #elif str( $genotype_likelihood_computation_type.perform_indel_calling.perform_indel_calling_selector ) == 'do_not_perform_indel_calling':
+            -I
+        #end if
+
+
+    #else:
+        ${genotype_likelihood_computation_type.base_position_on_reads}
+        ${genotype_likelihood_computation_type.output_mapping_quality}
+    #end if
+    --output "$output_mpileup" 2> "$output_log"
+    ]]>
+    </command>
+    <inputs>
+        <conditional name="reference_source">
+            <param label="Choose the source for the reference genome" name="reference_source_selector" type="select">
+                <option value="cached">Use a built-in genome</option>
+                <option value="history">Use a genome from the history</option>
+            </param>
+            <when value="cached">
+                <param format="bam" label="BAM file(s)" name="input_bam" type="data" min="1" multiple="True">
+                    <validator type="unspecified_build" />
+                    <validator message="Sequences are not currently available for the specified build." metadata_column="1" metadata_name="dbkey" table_name="fasta_indexes" type="dataset_metadata_in_data_table" />
+                </param>
+                <param label="Using reference genome" name="ref_file" type="select">
+                    <options from_data_table="fasta_indexes" />
+                </param>
+            </when>
+            <when value="history">
+                <param format="bam" label="BAM file(s)" name="input_bam" type="data" min="1" multiple="True">
+                    <validator check="bam_index" message="Metadata missing, click the pencil icon in the history item and use the auto-detect feature to correct this issue." type="metadata" />
+                </param>
+                <param format="fasta" label="Using reference genome" name="ref_file" type="data" />
+            </when>
+        </conditional>
+        <conditional name="genotype_likelihood_computation_type">
+            <param label="Genotype Likelihood Computation" name="genotype_likelihood_computation_type_selector" type="select">
+                <option selected="True" value="perform_genotype_likelihood_computation">Perform genotype likelihood computation (--VCF, --BCF options)</option>
+                <option value="do_not_perform_genotype_likelihood_computation">Do not perform genotype likelihood computation (output pileup)</option>
+            </param>
+            <when value="perform_genotype_likelihood_computation">
+                <param label="Choose the output format" name="output_format" type="select">
+                    <option value="--VCF">VCF</option>
+                    <option value="--BCF">BCF</option>
+                </param>
+                <param checked="False" falsevalue="--uncompressed" label="Compress output" name="compressed" truevalue="" type="boolean" help="--incompressed; default=False"/>
+                <param name="output_tags" optional="True" type="select" multiple="True" display="checkboxes" label="Optional tags to output" help="--output-tags">
+                    <option value="DP">DP (Number of high-quality bases)</option>
+                    <option value="DPR">DRP (Number of high-quality bases for each observed allele)</option>
+                    <option value="DV">DV (Number of high-quality non-reference bases)</option>
+                    <option value="DP4">DP4 (Number of high-quality ref-forward, ref-reverse, alt-forward and alt-reverse bases)</option>
+                    <option value="INFO/DPR">INFO/DPR (Number of high-quality bases for each observed allele)</option>
+                    <option value="SP">SP (Phred-scaled strand bias P-value)</option>
+                </param>
+                <conditional name="perform_indel_calling">
+                    <param label="Perform INDEL calling" name="perform_indel_calling_selector" type="select">
+                        <option selected="True" value="perform_indel_calling_def">Perform INDEL calling using default options</option>
+                        <option value="perform_indel_calling">Perform INDEL calling and set advanced options</option>
+                        <option value="do_not_perform_indel_calling">Do not perform INDEL calling</option>
+                    </param>
+                    <when value="perform_indel_calling_def" />
+                    <when value="perform_indel_calling">
+                        <param label="Phred-scaled gap open sequencing error probability" name="gap_open_sequencing_error_probability" type="integer" value="40" help="--open-prob; Reducing this value leads to more indel calls; default=40"/>
+                        <param label="Phred-scaled gap extension sequencing error probability" name="gap_extension_sequencing_error_probability" type="integer" value="20" help="--ext-prob;  Reducing this value leads to longer indels. default=20"/>
+                        <param label="Coefficient for modeling homopolymer errors." name="coefficient_for_modeling_homopolymer_errors" type="integer" value="100" help="--tandem-qual; default=100"/>
+                        <param label="Skip INDEL calling if the average per-sample depth is above" name="skip_indel_calling_above_sample_depth" type="integer" value="250" help="--max-idepth; default=250"/>
+                        <param label="Minimum gapped reads for indel candidates" name="minimum_gapped_reads_for_indel_candidates" type="integer" value="1" help="--min-ireads; default=1"/>
+                        <param label="Phred-scaled gap open sequencing error probability" name="open_seq_error_probability" type="integer" value="40" help="--open-prob; Reducing this value leads to more indel calls; default=40"/>
+                        <param label="Minimum fraction of gapped reads" name="minimum_gapped_read_fraction" type="float" value="0.002" help="--gap-frac; default=0.002"/>
+                        <param checked="False" falsevalue="" label="Apply --min-ireads and --gap-frac values on a per-sample basis" name="gapped_read_per_sample" truevalue="-p" type="boolean" help="--per-sample-mF;  by default both options are applied to reads pooled from all samples"/>
+                        <repeat name="platform_list_repeat" title="Platform for INDEL candidates">
+                            <param label="Platform to use for INDEL candidates" name="platform_entry" type="text" value="" help="It is recommended to collect indel candidates from sequencing technologies that have low indel error rate such as ILLUMINA"/>
+                        </repeat>
+                    </when>
+                    <when value="do_not_perform_indel_calling" />
+                </conditional>
+
+            </when>
+            <when value="do_not_perform_genotype_likelihood_computation">
+                <param checked="False" falsevalue="" label="Output base positions on reads" name="base_position_on_reads" truevalue="-O" type="boolean" help="--output-BP"/>
+                <param checked="False" falsevalue="" label="Output mapping quality" name="output_mapping_quality" truevalue="-s" type="boolean" help="--output-MQ"/>
+            </when>
+        </conditional>
+        <conditional name="advanced_options">
+            <param label="Set advanced options" name="advanced_options_selector" type="select">
+                <option selected="True" value="basic">Basic</option>
+                <option value="advanced">Advanced</option>
+            </param>
+            <when value="advanced">
+                <conditional name="filter_by_flags">
+                    <param label="Set filter by flags" name="filter_flags" type="select">
+                        <option selected="True" value="nofilter">Do not filter</option>
+                        <option value="filter">Filter by flags to exclude or require</option>
+                    </param>
+                    <when value="filter">
+                        <param display="checkboxes" label="Require" multiple="True" name="require_flags" type="select" help="--incl-flags">
+                            <option value="1">Read is paired</option>
+                            <option value="2">Read is mapped in a proper pair</option>
+                            <option value="4">The read is unmapped</option>
+                            <option value="8">The mate is unmapped</option>
+                            <option value="16">Read strand</option>
+                            <option value="32">Mate strand</option>
+                            <option value="64">Read is the first in a pair</option>
+                            <option value="128">Read is the second in a pair</option>
+                            <option value="256">The alignment or this read is not primary</option>
+                            <option value="512">The read fails platform/vendor quality checks</option>
+                            <option value="1024">The read is a PCR or optical duplicate</option>
+                        </param>
+                        <param display="checkboxes" label="Exclude" multiple="True" name="exclude_flags" type="select" help="--excl-flags">
+                            <option value="1">Read is paired</option>
+                            <option value="2">Read is mapped in a proper pair</option>
+                            <option value="4">The read is unmapped</option>
+                            <option value="8">The mate is unmapped</option>
+                            <option value="16">Read strand</option>
+                            <option value="32">Mate strand</option>
+                            <option value="64">Read is the first in a pair</option>
+                            <option value="128">Read is the second in a pair</option>
+                            <option value="256">The alignment or this read is not primary</option>
+                            <option value="512">The read fails platform/vendor quality checks</option>
+                            <option value="1024">The read is a PCR or optical duplicate</option>
+                        </param>
+                    </when>
+                    <when value="nofilter" />
+                </conditional>
+                <conditional name="limit_by_region">
+                    <param label="Select regions to call" name="limit_by_regions" type="select">
+                        <option selected="True" value="no_limit">Do not limit</option>
+                        <option value="history">From an uploaded BED file (--positions)</option>
+                        <option value="paste">Paste a list of regions or BED (--region)</option>
+                    </param>
+                    <when value="history">
+                        <param format="bed" label="BED file" name="bed_regions" type="data" help="--positions">
+                            <validator type="dataset_ok_validator" />
+                        </param>
+                    </when>
+                    <when value="paste">
+                        <param area="true" help="Paste a list of regions in BED format or as a list of chromosomes and positions" label="Regions" name="region_paste" size="10x35" type="text"/>
+                    </when>
+                    <when value="no_limit" />
+                </conditional>
+                <conditional name="exclude_read_group">
+                    <param label="Select read groups to exclude" name="exclude_read_groups" type="select" help="--exclude-RG">
+                        <option selected="True" value="no_limit">Do not exclude</option>
+                        <option value="history">From an uploaded text file</option>
+                        <option value="paste">Paste a list of read groups</option>
+                    </param>
+                    <when value="history">
+                        <param format="txt" label="Text file" name="read_groups" type="data">
+                            <validator type="dataset_ok_validator" />
+                        </param>
+                    </when>
+                    <when value="paste">
+                        <param area="true" help="Paste a list of read groups" label="Read groups" name="group_paste" size="10x35" type="text" />
+                    </when>
+                    <when value="no_limit" />
+                </conditional>
+                <param checked="False" falsevalue="" label="Disable read-pair overlap detection" name="ignore_overlaps" truevalue="-x" type="boolean" help="--ignore-overlaps"/>
+                <param checked="False" falsevalue="" label="Do not skip anomalous read pairs in variant calling" name="skip_anomalous_read_pairs" truevalue="-A" type="boolean" help="--count-orphans"/>
+                <param checked="False" falsevalue="" label="Disable probabilistic realignment for the computation of base alignment quality (BAQ)" name="disable_probabilistic_realignment" truevalue="-B" type="boolean" help="--no-BAQ; BAQ is the Phred-scaled probability of a read base being misaligned. Applying this option greatly helps to reduce false SNPs caused by misalignments"/>
+                <param label="Coefficient for downgrading mapping quality for reads containing excessive mismatches" name="coefficient_for_downgrading" type="integer" value="0" help="--adjust-MQ; Given a read with a phred-scaled probability q of being generated from the mapped position, the new mapping quality is about sqrt((INT-q)/INT)*INT. A zero value disables this functionality; if enabled, the recommended value for BWA is 50. default=0"/>
+                <param label="Max reads per BAM" max="1024" min="1" name="max_reads_per_bam" type="integer" value="250" help="--max-depth; default=250"/>
+                <param checked="False" falsevalue="" label="Redo BAQ computation" name="extended_BAQ_computation" truevalue="-E" type="boolean" help="--redo-BAQ; ignore existing BQ tags"/>
+                <param label="Minimum mapping quality for an alignment to be used" name="minimum_mapping_quality" type="integer" value="0" help="-min-MQ; default=0"/>
+                <param label="Minimum base quality for a base to be considered" name="minimum_base_quality" type="integer" value="13" help="--min-BQ; default=13"/>
+                <param label="Only generate pileup in region" name="region_string" type="text" value="" help="--region; If used in conjunction with --positions, then considers the intersection of the two requests. Defaults to all sites" />
+            </when>
+            <when value="basic" />
+        </conditional>
+    </inputs>
+    <outputs>
+        <data format="pileup" label="${tool.name} on ${on_string}" name="output_mpileup">
+            <change_format>
+                <when format="bcf" input="genotype_likelihood_computation_type.output_format" value="--BCF" />
+                <when format="vcf" input="genotype_likelihood_computation_type.output_format" value="--VCF" />
+            </change_format>
+        </data>
+        <data format="txt" label="${tool.name} on ${on_string} (log)" name="output_log" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="reference_source_selector" value="history" />
+            <param ftype="fasta" name="ref_file" value="phiX.fasta" />
+            <param ftype="bam" name="input_bam" value="samtools_mpileup_in_1.bam" />
+            <param name="genotype_likelihood_computation_type_selector" value="do_not_perform_genotype_likelihood_computation" />
+            <param name="advanced_options_selector" value="basic" />
+            <param name="base_position_on_reads" value="true" />
+            <param name="output_mapping_quality" value="true" />
+            <output file="samtools_mpileup_out_1.pileup" name="output_mpileup" />
+            <output file="samtools_mpileup_out_1.log" name="output_log" />
+        </test>
+        <test>
+            <param name="reference_source_selector" value="history" />
+            <param ftype="fasta" name="ref_file" value="phiX.fasta" />
+            <param ftype="bam" name="input_bam" value="phiX.bam" />
+            <param name="genotype_likelihood_computation_type_selector" value="perform_genotype_likelihood_computation" />
+            <param name="gap_extension_sequencing_error_probability" value="20" />
+            <param name="coefficient_for_modeling_homopolymer_errors" value="100" />
+            <param name="perform_indel_calling_selector" value="perform_indel_calling" />
+            <param name="skip_indel_calling_above_sample_depth" value="250" />
+            <param name="gap_open_sequencing_error_probability" value="40" />
+            <param name="platform_list_repeat" value="0" />
+            <param name="advanced_options_selector" value="basic" />
+            <param name="genotype_likelihood_computation_type|output_format" value="VCF" />
+            <output file="samtools_mpileup_out_2.vcf" ftype="vcf" lines_diff="8" name="output_mpileup" />
+            <output file="samtools_mpileup_out_2.log" name="output_log" />
+        </test>
+    </tests>
+    <help>
+<![CDATA[
+**What it does**
+
+Report variants for one or multiple BAM files. Alignment records are grouped by sample identifiers in @RG header lines. If sample identifiers are absent, each input file is regarded as one sample.
+
+------
+
+**Input options**::
+
+  -6, --illumina1.3+      quality is in the Illumina-1.3+ encoding
+  -A, --count-orphans     do not discard anomalous read pairs
+  -b, --bam-list FILE     list of input BAM filenames, one per line
+  -B, --no-BAQ            disable BAQ (per-Base Alignment Quality)
+  -C, --adjust-MQ INT     adjust mapping quality; recommended:50, disable:0 [0]
+  -d, --max-depth INT     max per-BAM depth; avoids excessive memory usage [250]
+  -E, --redo-BAQ          recalculate BAQ on the fly, ignore existing BQs
+  -f, --fasta-ref FILE    faidx indexed reference sequence file
+  -G, --exclude-RG FILE   exclude read groups listed in FILE
+  -l, --positions FILE    skip unlisted positions (chr pos) or regions (BED)
+  -q, --min-MQ INT        skip alignments with mapQ smaller than INT [0]
+  -Q, --min-BQ INT        skip bases with baseQ/BAQ smaller than INT [13]
+  -r, --region REG        region in which pileup is generated
+  -R, --ignore-RG         ignore RG tags (one BAM = one sample)
+  --rf, --incl-flags STR|INT  required flags: skip reads with mask bits unset []
+  --ff, --excl-flags STR|INT  filter flags: skip reads with mask bits set
+                                            [UNMAP,SECONDARY,QCFAIL,DUP]
+  -x, --ignore-overlaps   disable read-pair overlap detection
+
+**Output options**::
+
+  -o, --output FILE       write output to FILE [standard output]
+  -g, --BCF               generate genotype likelihoods in BCF format
+  -v, --VCF               generate genotype likelihoods in VCF format
+
+**Output options for mpileup format** (without -g/-v)::
+
+  -O, --output-BP         output base positions on reads
+  -s, --output-MQ         output mapping quality
+
+**Output options for genotype likelihoods** (when -g/-v is used)::
+
+  -t, --output-tags LIST  optional tags to output: DP,DPR,DV,DP4,INFO/DPR,SP []
+  -u, --uncompressed      generate uncompressed VCF/BCF output
+
+**SNP/INDEL genotype likelihoods options** (effective with -g/-v)::
+
+  -e, --ext-prob INT      Phred-scaled gap extension seq error probability [20]
+  -F, --gap-frac FLOAT    minimum fraction of gapped reads [0.002]
+  -h, --tandem-qual INT   coefficient for homopolymer errors [100]
+  -I, --skip-indels       do not perform indel calling
+  -L, --max-idepth INT    maximum per-sample depth for INDEL calling [250]
+  -m, --min-ireads INT    minimum number gapped reads for indel candidates [1]
+  -o, --open-prob INT     Phred-scaled gap open seq error probability [40]
+  -p, --per-sample-mF     apply -m and -F per-sample for increased sensitivity
+  -P, --platforms STR     comma separated list of platforms for indels [all]
+
+**Notes**: Assuming diploid individuals.
+]]>
+    </help>
+    <configfiles>
+        <configfile name="excluded_read_groups">
+<![CDATA[
+#set pasted_data = ''
+#if str( $advanced_options.advanced_options_selector ) == "advanced":
+    #if str( $advanced_options.exclude_read_group.exclude_read_groups ) == "paste":
+        #set pasted_data = '\t'.join( str( $advanced_options.exclude_read_group['read_groups'] ).split() )
+    #end if
+#end if
+${pasted_data}
+]]>
+        </configfile>
+        <configfile name="pasted_regions">
+<![CDATA[
+#set pasted_data = ''
+#if str( $advanced_options.advanced_options_selector ) == "advanced":
+    #if str( $advanced_options.limit_by_region.limit_by_regions ) == "paste":
+        #set pasted_data = '\t'.join( str( $advanced_options.limit_by_region['region_paste'] ).split() )
+    #end if
+#end if
+${pasted_data}
+]]>
+        </configfile>
+    </configfiles>
+    <expand macro="citations" />
+</tool>
diff --git a/tool_collections/samtools/samtools_mpileup/test-data/phiX.bam b/tool_collections/samtools/samtools_mpileup/test-data/phiX.bam
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a00000{{{d;LjnLB00RI3000000001Wf+)KH

literal 0
HcmV?d00001

diff --git a/tool_collections/samtools/samtools_mpileup/test-data/phiX.fasta b/tool_collections/samtools/samtools_mpileup/test-data/phiX.fasta
new file mode 100644
index 00000000000..53df885dc48
--- /dev/null
+++ b/tool_collections/samtools/samtools_mpileup/test-data/phiX.fasta
@@ -0,0 +1,79 @@
+>phiX174
+GAGTTTTATCGCTTCCATGACGCAGAAGTTAACACTTTCGGATATTTCTGATGAGTCGAAAAATTATCTT
+GATAAAGCAGGAATTACTACTGCTTGTTTACGAATTAAATCGAAGTGGACTGCTGGCGGAAAATGAGAAA
+ATTCGACCTATCCTTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATCAACTAACGATTCTG
+TCAAAAACTGACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGTTTA
+GATATGAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTGGATTACTATC
+TGAGTCCGATGCTGTTCAACCACTAATAGGTAAGAAATCATGAGTCAAGTTACTGAACAATCCGTACGTT
+TCCAGACCGCTTTGGCCTCTATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTAACCGAAGATGATTT
+CGATTTTCTGACGAGTAACAAAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCT
+TGCGTTTATGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTTTATTGCTGCCG
+TCATTGCTTATTATGTTCATCCCGTCAACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTAC
+GGAAAACATTATTAATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGTA
+CGCGCAGGAAACACTGACGTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAAATTACGTGCAGAAGGAG
+TGATGTAATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACT
+AAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGC
+CCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCA
+TCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATTTCAACTACTCCGGTTATCGCTGGCGAC
+TCCTTCGAGATGGACGCCGTTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTA
+CTGTAGACATTTTTACTTTTTATGTCCCTCATCGTCACGTTTATGGTGAACAGTGGATTAAGTTCATGAA
+GGATGGTGTTAATGCCACTCCTCTCCCGACTGTTAACACTACTGGTTATATTGACCATGCCGCTTTTCTT
+GGCACGATTAACCCTGATACCAATAAAATCCCTAAGCATTTGTTTCAGGGTTATTTGAATATCTATAACA
+ACTATTTTAAAGCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATCAAGATGATGC
+TCGTTATGGTTTCCGTTGCTGCCATCTCAAAAACATTTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTT
+TCTCGCCAAATGACGACTTCTACCACATCTATTGACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGC
+ATACTGACCAAGAACGTGATTACTTCATGCAGCGTTACCGTGATGTTATTTCTTCATTTGGAGGTAAAAC
+CTCTTATGACGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCTATGATGTT
+GATGGAACTGACCAAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAACATTCTGTGC
+CGCGTTTCTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGCGAC
+TAAAGAGATTCAGTACCTTAACGCTAAAGGTGCTTTGACTTATACCGATATTGCTGGCGACCCTGTTTTG
+TATGGCAACTTGCCGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGATTCGTCTAAGAAGT
+TTAAGATTGCTGAGGGTCAGTGGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCTTCTTGA
+AGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGAT
+TATGACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGAATAGTCAGGTTAAATTTAATGTGACCGTTT
+ATCGCAATCTGCCGACCACTCGCGATTCAATCATGACTTCGTGATAAAAGATTGAGTGTGAGGTTATAAC
+GCCGAAGCGGTAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACCAAGCGAAGCGCGGTAGGTTTTCTGC
+TTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTCGCCATAATTCAAACTTTTTTTCTGATAAGCTGGT
+TCTCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTTTACAGACACCTAAAGCTACATCGTCAACGTTA
+TATTTTGATAGTTTGACGGTTAATGCTGGTAATGGTGGTTTTCTTCATTGCATTCAGATGGATACATCTG
+TCAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGCCGACCCTAAATTTTTTGC
+CTGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTCCCGACTGCCTATGATGTTTATCCTTTG
+AATGGTCGCCATGATGGTGGTTATTATACCGTCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTACGC
+CGGGCAATAATGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTACTAAATGCCGCGGATTGGT
+TTCGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGGTGATTTATGTTTGGTG
+CTATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAA
+AGCCGCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACCGATAACAATACTGTAGGCATGGGTGATGCT
+GGTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCCCCTAGTTTTGTTTCTG
+GTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGGCACTTCTGCCGTTTCTGA
+TAAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGATTAT
+CTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGG
+TTGACGCCGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCAACTGGACAATCAGAAAGAGATTGCCGA
+GATGCAAAATGAGACTCAAAAAGAGATTGCTGGCATTCAGTCGGCGACTTCACGCCAGAATACGAAAGAC
+CAGGTATATGCACAAAATGAGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGCGTTGCGTCTATTA
+TGGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAAGCTCA
+AACGGCTGGTCAGTATTTTACCAATGACCAAATCAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGAC
+TTAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCTCTTCTCATATTGGCGCTACTGCAAAGGATATTT
+CTAATGTCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTTTTCATGGTATTGATAAAGCTGTTGCCGA
+TACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTCTAATTTGTCTAGGAAATAACCG
+TCAGGATTGACACCCTCCCAATTGTATGTTTTCATGCCTCCAAATCTTGGAGGCTTTTTTATGGTTCGTT
+CTTATTACCCTTCTGAATGTCACGCTGATTATTTTGACTTTGAGCGTATCGAGGCTCTTAAACCTGCTAT
+TGAGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAATGCTTGGCTTCCATAAGCAGATGGATAACCGC
+ATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAGGGCGTTGAGTTCGATAATGGTGATATGTATG
+TTGACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTACTGAGAAGTTAATGGATGA
+ATTGGCACAATGCTACAATGTGCTCCCCCAACTTGATATTAATAACACTATAGACCACCGCCCCGAAGGG
+GACGAAAAATGGTTTTTAGAGAACGAGAAGACGGTTACGCAGTTTTGCCGCAAGCTGGCTGCTGAACGCC
+CTCTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCAAGATT
+GCTGGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTACTATTCAGCGTTTGATGAATGCAATGCGACAG
+GCTCATGCTGATGGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCTGACGACCGATTAGAGGCGTTTT
+ATGATAATCCCAATGCTTTGCGTGACTATTTTCGTGATATTGGTCGTATGGTTCTTGCTGCCGAGGGTCG
+CAAGGCTAATGATTCACACGCCGACTGCTATCAGTATTTTTGTGTGCCTGAGTATGGTACAGCTAATGGC
+CGTCTTCATTTCCATGCGGTGCATTTTATGCGGACACTTCCTACAGGTAGCGTTGACCCTAATTTTGGTC
+GTCGGGTACGCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGTGGCCTTATGGTTACAGTATGCCCAT
+CGCAGTTCGCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAG
+CCGCTTAAAGCTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTAAATACGTTAACAAAAAGTCAGATA
+TGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCACTAAAAACCAAGCTGTCGCTACT
+TCCCAAGAAGCTGTTCAGAATCAGAATGAGCCGCAACTTCGGGATGAAAATGCTCACAATGACAAATCTG
+TCCACGGAGTGCTTAATCCAACTTACCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGATATTGAAGC
+AGAACGCAAAAAGAGAGATGAGATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTTTGGCGGCGCAACC
+TGTGACGACAAATCTGCTCAAATTTATGCGCGCTTCGATAAAAATGATTGGCGTATCCAACCTGCA
+
diff --git a/tool_collections/samtools/samtools_mpileup/test-data/phiX_1.bam b/tool_collections/samtools/samtools_mpileup/test-data/phiX_1.bam
new file mode 100644
index 0000000000000000000000000000000000000000..a1aae19efa4c9ce3805a7af169ae1c7a01fb4812
GIT binary patch
literal 5319
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z;bH_D$^pbstqfeE3=9A&>0<$H0000ViwFb&00000{{{d;LjnLY6vdoPtSrY_hUeaE
z`}*(NuA-t8A||d@5lWVjrvK*00;H<xFl8YdgU6EX&53P{AggR5Awd>Z6rzIo5sNI?
z7#Rt%LBe8{D1ik+GC^1Z3F0^j!Nx3%42dk+v0`y;zMAenr+TKleJu649Qn?@p4B|(
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diff --git a/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_1.pileup b/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_1.pileup
new file mode 100644
index 00000000000..b75cb580982
--- /dev/null
+++ b/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_1.pileup
@@ -0,0 +1,43 @@
+phiX174	1411	A	0				1
+phiX174	1412	G	0				2,1,1
+phiX174	1413	C	0				3,2,2,1,1
+phiX174	1414	G	0				4,3,3,2,2,1
+phiX174	1415	C	0				5,4,4,3,3,2,1
+phiX174	1416	C	0				6,5,5,4,4,3,2,1
+phiX174	1417	G	0				7,6,6,5,5,4,3,2,1
+phiX174	1418	T	0				8,7,7,6,6,5,4,3,2,1
+phiX174	1419	G	0				9,8,8,7,7,6,5,4,3,2
+phiX174	1420	G	0				10,9,9,8,8,7,6,5,4,3
+phiX174	1421	A	0				11,10,10,9,9,8,7,6,5,4
+phiX174	1422	T	0				12,11,11,10,10,9,8,7,6,5
+phiX174	1423	G	0				13,12,12,11,11,10,9,8,7,6
+phiX174	1424	C	0				14,13,13,12,12,11,10,9,8,7
+phiX174	1425	C	0				15,14,14,13,13,12,11,10,9,8
+phiX174	1426	T	0				16,15,15,14,14,13,12,11,10,9
+phiX174	1427	G	0				17,16,16,15,15,14,13,12,11,10
+phiX174	1428	A	0				18,17,17,16,16,15,14,13,12,11
+phiX174	1429	C	0				19,18,18,17,17,16,15,14,13,12
+phiX174	1430	C	0				20,19,19,18,18,17,16,15,14,13
+phiX174	1431	G	0				21,20,20,19,19,18,17,16,15,14
+phiX174	1432	T	0				22,21,21,20,20,19,18,17,16,15
+phiX174	1433	A	0				23,22,22,21,21,20,19,18,17,16
+phiX174	1434	C	0				24,23,23,22,22,21,20,19,18,17
+phiX174	1435	C	0				25,24,24,23,23,22,21,20,19,18
+phiX174	1436	G	0				26,25,25,24,24,23,22,21,20,19
+phiX174	1437	A	0				27,26,26,25,25,24,23,22,21,20
+phiX174	1438	G	0				28,27,27,26,26,25,24,23,22,21
+phiX174	1439	G	0				29,28,28,27,27,26,25,24,23,22
+phiX174	1440	C	0				30,29,29,28,28,27,26,25,24,23
+phiX174	1441	T	0				31,30,30,29,29,28,27,26,25,24
+phiX174	1442	A	0				32,31,31,30,30,29,28,27,26,25
+phiX174	1443	A	0				33,32,32,31,31,30,29,28,27,26
+phiX174	1444	C	0				34,33,33,32,32,31,30,29,28,27
+phiX174	1445	C	0				34,33,34,32,33,32,31,30,29,28
+phiX174	1446	C	0				35,34,35,33,34,33,32,31,30,29
+phiX174	1447	T	0				36,35,36,34,35,34,33,32,31,30
+phiX174	1448	A	0				36,35,36,35,34,33,32,31
+phiX174	1449	A	0				36,36,35,34,33,32
+phiX174	1450	T	0				36,35,34,33
+phiX174	1451	G	0				36,35,34
+phiX174	1452	A	0				36,35
+phiX174	1453	G	0				36
diff --git a/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_2.vcf b/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_2.vcf
new file mode 100644
index 00000000000..e9fdae00908
--- /dev/null
+++ b/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_2.vcf
@@ -0,0 +1,22 @@
+##fileformat=VCFv4.2
+##FILTER=<ID=PASS,Description="All filters passed">
+##samtoolsVersion=1.1+htslib-1.1
+##samtoolsCommand=samtools mpileup --VCF --uncompressed -f /tmp/tmpId8vOP/tmp3bubIE/database/files/000/dataset_735.dat -g -e 20 -h 100 -L 250 -m 1 --open-prob 40 -F 0.002 -e 40 --output /tmp/tmpId8vOP/tmp3bubIE/database/files/000/dataset_736.dat /tmp/tmpId8vOP/tmp3bubIE/database/files/000/dataset_734.dat
+##reference=file:///tmp/tmpId8vOP/tmp3bubIE/database/files/000/dataset_735.dat
+##contig=<ID=phiX174,length=5386>
+##ALT=<ID=X,Description="Represents allele(s) other than observed.">
+##INFO=<ID=INDEL,Number=0,Type=Flag,Description="Indicates that the variant is an INDEL.">
+##INFO=<ID=IDV,Number=1,Type=Integer,Description="Maximum number of reads supporting an indel">
+##INFO=<ID=IMF,Number=1,Type=Float,Description="Maximum fraction of reads supporting an indel">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Raw read depth">
+##INFO=<ID=VDB,Number=1,Type=Float,Description="Variant Distance Bias for filtering splice-site artefacts in RNA-seq data (bigger is better)",Version="3">
+##INFO=<ID=RPB,Number=1,Type=Float,Description="Mann-Whitney U test of Read Position Bias (bigger is better)">
+##INFO=<ID=MQB,Number=1,Type=Float,Description="Mann-Whitney U test of Mapping Quality Bias (bigger is better)">
+##INFO=<ID=BQB,Number=1,Type=Float,Description="Mann-Whitney U test of Base Quality Bias (bigger is better)">
+##INFO=<ID=MQSB,Number=1,Type=Float,Description="Mann-Whitney U test of Mapping Quality vs Strand Bias (bigger is better)">
+##INFO=<ID=SGB,Number=1,Type=Float,Description="Segregation based metric.">
+##INFO=<ID=MQ0F,Number=1,Type=Float,Description="Fraction of MQ0 reads (smaller is better)">
+##INFO=<ID=I16,Number=16,Type=Float,Description="Auxiliary tag used for calling, see description of bcf_callret1_t in bam2bcf.h">
+##INFO=<ID=QS,Number=R,Type=Float,Description="Auxiliary tag used for calling">
+##FORMAT=<ID=PL,Number=G,Type=Integer,Description="List of Phred-scaled genotype likelihoods">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	/tmp/tmpId8vOP/tmp3bubIE/database/files/000/dataset_734.dat
diff --git a/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_4.vcf b/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_4.vcf
new file mode 100644
index 00000000000..ddc2d30ad2e
--- /dev/null
+++ b/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_4.vcf
@@ -0,0 +1,22 @@
+##fileformat=VCFv4.2
+##FILTER=<ID=PASS,Description="All filters passed">
+##samtoolsVersion=1.1+htslib-1.1
+##samtoolsCommand=samtools mpileup --VCF --uncompressed -f /var/galaxy/workspace/CleanGalaxy/tool-data/phiX/sam_indexes/phiX/phiX.fa -C 0 -d 200 -E -q 0 -Q 43 -g -e 20 -h 100 -L 250 -m 1 --open-prob 40 -F 0.002 -e 40 --output /tmp/tmp5TzrZC/tmpDtGVov/database/files/000/dataset_756.dat /tmp/tmp5TzrZC/tmpDtGVov/database/files/000/dataset_755.dat
+##reference=file:///var/galaxy/workspace/CleanGalaxy/tool-data/phiX/sam_indexes/phiX/phiX.fa
+##contig=<ID=phiX174,length=5386>
+##ALT=<ID=X,Description="Represents allele(s) other than observed.">
+##INFO=<ID=INDEL,Number=0,Type=Flag,Description="Indicates that the variant is an INDEL.">
+##INFO=<ID=IDV,Number=1,Type=Integer,Description="Maximum number of reads supporting an indel">
+##INFO=<ID=IMF,Number=1,Type=Float,Description="Maximum fraction of reads supporting an indel">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Raw read depth">
+##INFO=<ID=VDB,Number=1,Type=Float,Description="Variant Distance Bias for filtering splice-site artefacts in RNA-seq data (bigger is better)",Version="3">
+##INFO=<ID=RPB,Number=1,Type=Float,Description="Mann-Whitney U test of Read Position Bias (bigger is better)">
+##INFO=<ID=MQB,Number=1,Type=Float,Description="Mann-Whitney U test of Mapping Quality Bias (bigger is better)">
+##INFO=<ID=BQB,Number=1,Type=Float,Description="Mann-Whitney U test of Base Quality Bias (bigger is better)">
+##INFO=<ID=MQSB,Number=1,Type=Float,Description="Mann-Whitney U test of Mapping Quality vs Strand Bias (bigger is better)">
+##INFO=<ID=SGB,Number=1,Type=Float,Description="Segregation based metric.">
+##INFO=<ID=MQ0F,Number=1,Type=Float,Description="Fraction of MQ0 reads (smaller is better)">
+##INFO=<ID=I16,Number=16,Type=Float,Description="Auxiliary tag used for calling, see description of bcf_callret1_t in bam2bcf.h">
+##INFO=<ID=QS,Number=R,Type=Float,Description="Auxiliary tag used for calling">
+##FORMAT=<ID=PL,Number=G,Type=Integer,Description="List of Phred-scaled genotype likelihoods">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	/tmp/tmp5TzrZC/tmpDtGVov/database/files/000/dataset_755.dat
diff --git a/tool_collections/samtools/samtools_mpileup/tool-data/fasta_indexes.loc.sample b/tool_collections/samtools/samtools_mpileup/tool-data/fasta_indexes.loc.sample
new file mode 100644
index 00000000000..fd8fb70ac28
--- /dev/null
+++ b/tool_collections/samtools/samtools_mpileup/tool-data/fasta_indexes.loc.sample
@@ -0,0 +1,29 @@
+#This is a sample file distributed with Galaxy that enables tools
+#to use a directory of Samtools indexed sequences data files.  You will need
+#to create these data files and then create a fasta_indexes.loc file
+#similar to this one (store it in this directory) that points to
+#the directories in which those files are stored. The fasta_indexes.loc
+#file has this format (white space characters are TAB characters):
+#
+# <unique_build_id>	<dbkey>	<display_name>	<file_base_path>
+#
+#So, for example, if you had hg19 Canonical indexed stored in
+#
+# /depot/data2/galaxy/hg19/sam/,
+#
+#then the fasta_indexes.loc entry would look like this:
+#
+#hg19canon	hg19	Human (Homo sapiens): hg19 Canonical	/depot/data2/galaxy/hg19/sam/hg19canon.fa
+#
+#and your /depot/data2/galaxy/hg19/sam/ directory
+#would contain hg19canon.fa and hg19canon.fa.fai files.
+#
+#Your fasta_indexes.loc file should include an entry per line for
+#each index set you have stored.  The file in the path does actually
+#exist, but it should never be directly used. Instead, the name serves
+#as a prefix for the index file.  For example:
+#
+#hg18canon	hg18	Human (Homo sapiens): hg18 Canonical	/depot/data2/galaxy/hg18/sam/hg18canon.fa
+#hg18full	hg18	Human (Homo sapiens): hg18 Full	/depot/data2/galaxy/hg18/sam/hg18full.fa
+#hg19canon	hg19	Human (Homo sapiens): hg19 Canonical	/depot/data2/galaxy/hg19/sam/hg19canon.fa
+#hg19full	hg19	Human (Homo sapiens): hg19 Full	/depot/data2/galaxy/hg19/sam/hg19full.fa
diff --git a/tool_collections/samtools/samtools_mpileup/tool_data_table_conf.xml.sample b/tool_collections/samtools/samtools_mpileup/tool_data_table_conf.xml.sample
new file mode 100644
index 00000000000..c34ade01890
--- /dev/null
+++ b/tool_collections/samtools/samtools_mpileup/tool_data_table_conf.xml.sample
@@ -0,0 +1,7 @@
+<!-- Use the file tool_data_table_conf.xml.oldlocstyle if you don't want to update your loc files as changed in revision 4550:535d276c92bc-->
+<tables>
+    <table name="fasta_indexes" comment_char="#">
+        <columns>value, dbkey, name, path</columns>
+        <file path="tool-data/fasta_indexes.loc" />
+    </table>
+</tables>
diff --git a/tool_collections/samtools/samtools_mpileup/tool_dependencies.xml b/tool_collections/samtools/samtools_mpileup/tool_dependencies.xml
new file mode 120000
index 00000000000..b11b378d63c
--- /dev/null
+++ b/tool_collections/samtools/samtools_mpileup/tool_dependencies.xml
@@ -0,0 +1 @@
+../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_phase/.shed.yml b/tool_collections/samtools/samtools_phase/.shed.yml
new file mode 100644
index 00000000000..da1b900371a
--- /dev/null
+++ b/tool_collections/samtools/samtools_phase/.shed.yml
@@ -0,0 +1,9 @@
+categories:
+- SAM
+- Variant Analysis
+description: Call and phase heterozygous SNPs
+name: samtools_phase
+owner: devteam
+homepage_url: http://www.htslib.org/
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_phase
+type: unrestricted
diff --git a/tool_collections/samtools/samtools_phase/macros.xml b/tool_collections/samtools/samtools_phase/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/samtools/samtools_phase/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_phase/samtools_phase.xml b/tool_collections/samtools/samtools_phase/samtools_phase.xml
new file mode 100644
index 00000000000..eb89119e03c
--- /dev/null
+++ b/tool_collections/samtools/samtools_phase/samtools_phase.xml
@@ -0,0 +1,130 @@
+<tool id="samtools_phase" name="Call and phase" version="2.0">
+    <description>heterozygous SNPs</description>
+    <macros>
+    <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"></expand>
+  <expand macro="stdio"></expand>
+  <expand macro="version_command"></expand>
+    <command>samtools phase -b "phase_wrapper"
+        #if str($option_set.option_sets) == 'advanced':
+            ${option_set.ignore_chimeras}
+            -k $option_set.block_length
+            -q $option_set.min_het
+            -Q $option_set.min_bq
+            -D $option_set.read_depth
+            ${option_set.drop_ambiguous}
+        #else
+            -k 13 -q 37 -Q 13 -D 256
+        #end if
+        "$input_bam" &gt; "$phase_sets"
+    </command>
+    <inputs>
+        <param format="bam" name="input_bam" type="data" label="Select dataset to phase"/>
+        <conditional name="option_set">
+            <param name="option_sets" type="select" label="Phase parameters">
+                <option value="default">Use defaults</option>
+                <option value="advanced">Advanced options</option>
+            </param>
+            <when value="default" />
+            <when value="advanced">
+                <param name="block_length" type="integer" value="13" label="Maximum length for local phasing" />
+                <param name="min_het" type="integer" value="37" label="Minimum Phred-scaled level of detail to call a heterozygote" />
+                <param name="min_bq" type="integer" value="13" label="Minimum base quality to be used in het calling" />
+                <param name="read_depth" type="integer" value="256" label="Read depth" />
+                <param name="ignore_chimeras" type="boolean" truevalue="-F" falsevalue="" checked="False" label="Do not attempt to fix chimeric reads" />
+                <param name="drop_ambiguous" type="boolean" truevalue="-A 1" falsevalue="" checked="False" label="Drop reads with ambiguous phase" />
+            </when>
+        </conditional>
+    </inputs>
+    <outputs>
+        <data format="txt" name="phase_sets" />
+        <data format="bam" from_work_dir="phase_wrapper.0.bam" name="phase0" label="${tool.name} on ${on_string}: Phase-0 reads" />
+        <data format="bam" from_work_dir="phase_wrapper.1.bam" name="phase1" label="${tool.name} on ${on_string}: Phase-1 reads" />
+        <data format="bam" from_work_dir="phase_wrapper.chimeras.bam" name="chimera" label="${tool.name} on ${on_string}: Chimeric reads" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="option_sets" value="default" /> 
+            <param name="input_bam" value="samtools_phase_in_1.bam" /> 
+            <output name="phase_sets" file="samtools_phase_out_1_log.txt" ftype="txt" />
+            <output name="phase0" file="samtools_phase_out_1_phase0.bam" ftype="bam" />
+            <output name="phase1" file="samtools_phase_out_1_phase1.bam" ftype="bam" />
+            <output name="chimera" file="empty_file.bam" compare="contains" />
+        </test>
+        <test>
+            <param name="input_bam" value="samtools_phase_in_2.bam" /> 
+            <param name="option_sets" value="advanced" /> 
+            <param name="option_set|block_length" value="13" />
+            <param name="option_set|min_het" value="37" />
+            <param name="option_set|min_bq" value="13" />
+            <param name="option_set|read_depth" value="256" />
+            <param name="option_set|ignore_chimeras" value="false" />
+            <param name="option_set|drop_ambiguous" value="true" />
+            <output name="phase_sets" file="samtools_phase_out_2_log.txt" ftype="txt" />
+            <output name="phase0" file="samtools_phase_out_2_phase0.bam" ftype="bam" />
+            <output name="phase1" file="samtools_phase_out_2_phase1.bam" ftype="bam" />
+            <output name="chimera" file="empty_file.bam" compare="contains" />
+        </test>
+        <test>
+            <param name="input_bam" value="samtools_phase_in_2.bam" /> 
+            <param name="option_sets" value="advanced" /> 
+            <param name="option_set|block_length" value="13" />
+            <param name="option_set|min_het" value="37" />
+            <param name="option_set|min_bq" value="13" />
+            <param name="option_set|read_depth" value="256" />
+            <param name="option_set|ignore_chimeras" value="true" />
+            <param name="option_set|drop_ambiguous" value="false" />
+            <output name="phase_sets" file="samtools_phase_out_3_log.txt" ftype="txt" />
+            <output name="phase0" file="samtools_phase_out_3_phase0.bam" ftype="bam" />
+            <output name="phase1" file="samtools_phase_out_3_phase1.bam" ftype="bam" />
+            <output name="chimera" file="empty_file.bam" compare="contains" />
+        </test>
+    </tests>
+    <help>
+**What it does**
+
+Call and phase heterozygous SNPs
+    
+------
+
+.. list-table:: **Options**
+   :widths: 5 5 40 10
+   :header-rows: 1
+
+   * - Flag
+     - Type
+     - Description
+     - Default
+   * - -k
+     - INT
+     - Block length
+     - 13
+   * - -b
+     - STR
+     - Prefix of BAM file output
+     - *null*
+   * - -q
+     - INT
+     - Minimum het phred-LOD
+     - 37
+   * - -Q
+     - INT
+     - Min base quality in het calling
+     - 13
+   * - -D
+     - INT
+     - Max read depth
+     - 256
+   * - -D
+     - BOOLEAN
+     - Do not attempt to fix chimeras
+     - *off*
+   * - -A
+     - BOOLEAN
+     - Drop reads with ambiguous phase
+     - *off*
+
+    </help>
+    <expand macro="citations"></expand>
+</tool>
diff --git a/tool_collections/samtools/samtools_phase/test-data/empty_file.bam b/tool_collections/samtools/samtools_phase/test-data/empty_file.bam
new file mode 100644
index 00000000000..e69de29bb2d
diff --git a/tool_collections/samtools/samtools_phase/test-data/samtools_phase_in_1.bam b/tool_collections/samtools/samtools_phase/test-data/samtools_phase_in_1.bam
new file mode 100644
index 0000000000000000000000000000000000000000..340e7823c569b869e62d95802a6161f0a4bea089
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diff --git a/tool_collections/samtools/samtools_phase/test-data/samtools_phase_in_2.bam b/tool_collections/samtools/samtools_phase/test-data/samtools_phase_in_2.bam
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literal 0
HcmV?d00001

diff --git a/tool_collections/samtools/samtools_phase/test-data/samtools_phase_out_1_log.txt b/tool_collections/samtools/samtools_phase/test-data/samtools_phase_out_1_log.txt
new file mode 100644
index 00000000000..a2f4cd4a214
--- /dev/null
+++ b/tool_collections/samtools/samtools_phase/test-data/samtools_phase_out_1_log.txt
@@ -0,0 +1,30 @@
+CC
+CC	Descriptions:
+CC
+CC	  CC      comments
+CC	  PS      start of a phase set
+CC	  FL      filtered region
+CC	  M[012]  markers; 0 for singletons, 1 for phased and 2 for filtered
+CC	  EV      supporting reads; SAM format
+CC	  //      end of a phase set
+CC
+CC	Formats of PS, FL and M[012] lines (1-based coordinates):
+CC
+CC	  PS  chr  phaseSetStart  phaseSetEnd
+CC	  FL  chr  filterStart    filterEnd
+CC	  M?  chr  PS  pos  allele0  allele1  hetIndex  #supports0  #errors0  #supp1  #err1
+CC
+CC
+PS	chrI	1187	1290
+M1	chrI	1187	1187	T	A	1	2	0	4	0
+M1	chrI	1187	1216	T	A	2	3	0	4	0
+M1	chrI	1187	1222	C	A	3	3	0	3	1
+M1	chrI	1187	1290	A	T	4	1	0	0	0
+EV	0	chrI	1	40	3M	*	0	0	AAA	*	YP:i:1	YF:i:0	YI:i:3	YO:i:0	YS:i:1145
+EV	0	chrI	1	40	3M	*	0	0	TTC	*	YP:i:0	YF:i:0	YI:i:3	YO:i:0	YS:i:1157
+EV	0	chrI	1	40	3M	*	0	0	TTC	*	YP:i:0	YF:i:0	YI:i:3	YO:i:0	YS:i:1141
+EV	0	chrI	1	40	3M	*	0	0	AAA	*	YP:i:1	YF:i:0	YI:i:3	YO:i:0	YS:i:1147
+EV	0	chrI	1	40	3M	*	0	0	AAA	*	YP:i:1	YF:i:0	YI:i:3	YO:i:0	YS:i:1176
+EV	0	chrI	1	40	3M	*	0	0	AAC	*	YP:i:1	YF:i:0	YI:i:2	YO:i:1	YS:i:1164
+EV	0	chrI	2	40	3M	*	0	0	TCA	*	YP:i:0	YF:i:0	YI:i:3	YO:i:0	YS:i:1215
+//
diff --git a/tool_collections/samtools/samtools_phase/test-data/samtools_phase_out_1_phase0.bam b/tool_collections/samtools/samtools_phase/test-data/samtools_phase_out_1_phase0.bam
new file mode 100644
index 0000000000000000000000000000000000000000..14d56ffce89f0e52f8dcc4609d43b7bc2ae52423
GIT binary patch
literal 1801
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literal 0
HcmV?d00001

diff --git a/tool_collections/samtools/samtools_phase/test-data/samtools_phase_out_1_phase1.bam b/tool_collections/samtools/samtools_phase/test-data/samtools_phase_out_1_phase1.bam
new file mode 100644
index 0000000000000000000000000000000000000000..557a4c50fbdec6a00d647c1bd98e183748331968
GIT binary patch
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literal 0
HcmV?d00001

diff --git a/tool_collections/samtools/samtools_phase/test-data/samtools_phase_out_2_log.txt b/tool_collections/samtools/samtools_phase/test-data/samtools_phase_out_2_log.txt
new file mode 100644
index 00000000000..f307b6c0346
--- /dev/null
+++ b/tool_collections/samtools/samtools_phase/test-data/samtools_phase_out_2_log.txt
@@ -0,0 +1,65 @@
+CC
+CC	Descriptions:
+CC
+CC	  CC      comments
+CC	  PS      start of a phase set
+CC	  FL      filtered region
+CC	  M[012]  markers; 0 for singletons, 1 for phased and 2 for filtered
+CC	  EV      supporting reads; SAM format
+CC	  //      end of a phase set
+CC
+CC	Formats of PS, FL and M[012] lines (1-based coordinates):
+CC
+CC	  PS  chr  phaseSetStart  phaseSetEnd
+CC	  FL  chr  filterStart    filterEnd
+CC	  M?  chr  PS  pos  allele0  allele1  hetIndex  #supports0  #errors0  #supp1  #err1
+CC
+CC
+PS	chrI	1412	1542
+M1	chrI	1412	1412	T	C	1	7	0	9	0
+M1	chrI	1412	1414	G	A	2	7	0	9	0
+M1	chrI	1412	1421	G	A	3	7	0	9	0
+M1	chrI	1412	1471	T	C	4	5	0	6	0
+M1	chrI	1412	1542	C	T	5	3	0	2	0
+EV	0	chrI	1	40	3M	*	0	0	TGG	*	YP:i:0	YF:i:0	YI:i:3	YO:i:0	YS:i:1366
+EV	0	chrI	1	40	3M	*	0	0	CAA	*	YP:i:1	YF:i:0	YI:i:3	YO:i:0	YS:i:1343
+EV	0	chrI	1	40	4M	*	0	0	TGGT	*	YP:i:0	YF:i:0	YI:i:4	YO:i:0	YS:i:1409
+EV	0	chrI	1	40	3M	*	0	0	CAA	*	YP:i:1	YF:i:0	YI:i:3	YO:i:0	YS:i:1353
+EV	0	chrI	1	40	3M	*	0	0	TGG	*	YP:i:0	YF:i:0	YI:i:3	YO:i:0	YS:i:1358
+EV	0	chrI	1	40	4M	*	0	0	CAAC	*	YP:i:1	YF:i:0	YI:i:4	YO:i:0	YS:i:1374
+EV	0	chrI	1	40	3M	*	0	0	CAA	*	YP:i:1	YF:i:0	YI:i:3	YO:i:0	YS:i:1350
+EV	0	chrI	1	40	4M	*	0	0	CAAC	*	YP:i:1	YF:i:0	YI:i:4	YO:i:0	YS:i:1379
+EV	0	chrI	1	40	4M	*	0	0	CAAC	*	YP:i:1	YF:i:0	YI:i:4	YO:i:0	YS:i:1389
+EV	0	chrI	1	40	3M	*	0	0	TGG	*	YP:i:0	YF:i:0	YI:i:3	YO:i:0	YS:i:1341
+EV	0	chrI	1	40	3M	*	0	0	TGG	*	YP:i:0	YF:i:0	YI:i:3	YO:i:0	YS:i:1340
+EV	0	chrI	1	40	3M	*	0	0	CAA	*	YP:i:1	YF:i:0	YI:i:3	YO:i:0	YS:i:1359
+EV	0	chrI	1	40	4M	*	0	0	CAAC	*	YP:i:1	YF:i:0	YI:i:4	YO:i:0	YS:i:1392
+EV	0	chrI	1	40	4M	*	0	0	TGGT	*	YP:i:0	YF:i:0	YI:i:4	YO:i:0	YS:i:1407
+EV	0	chrI	1	40	3M	*	0	0	TGG	*	YP:i:0	YF:i:0	YI:i:3	YO:i:0	YS:i:1346
+EV	0	chrI	1	40	3M	*	0	0	CAA	*	YP:i:1	YF:i:0	YI:i:3	YO:i:0	YS:i:1348
+EV	0	chrI	4	40	2M	*	0	0	TC	*	YP:i:0	YF:i:0	YI:i:2	YO:i:0	YS:i:1444
+EV	0	chrI	4	40	2M	*	0	0	CT	*	YP:i:1	YF:i:0	YI:i:2	YO:i:0	YS:i:1462
+EV	0	chrI	4	40	2M	*	0	0	CT	*	YP:i:1	YF:i:0	YI:i:2	YO:i:0	YS:i:1457
+EV	0	chrI	4	40	2M	*	0	0	TC	*	YP:i:0	YF:i:0	YI:i:2	YO:i:0	YS:i:1468
+EV	0	chrI	4	40	2M	*	0	0	TC	*	YP:i:0	YF:i:0	YI:i:2	YO:i:0	YS:i:1461
+//
+PS	chrI	3611	3731
+M1	chrI	3611	3611	T	A	6	5	0	4	0
+M1	chrI	3611	3659	C	T	7	9	0	6	0
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diff --git a/tool_collections/samtools/samtools_phase/test-data/samtools_phase_out_3_log.txt b/tool_collections/samtools/samtools_phase/test-data/samtools_phase_out_3_log.txt
new file mode 100644
index 00000000000..f307b6c0346
--- /dev/null
+++ b/tool_collections/samtools/samtools_phase/test-data/samtools_phase_out_3_log.txt
@@ -0,0 +1,65 @@
+CC
+CC	Descriptions:
+CC
+CC	  CC      comments
+CC	  PS      start of a phase set
+CC	  FL      filtered region
+CC	  M[012]  markers; 0 for singletons, 1 for phased and 2 for filtered
+CC	  EV      supporting reads; SAM format
+CC	  //      end of a phase set
+CC
+CC	Formats of PS, FL and M[012] lines (1-based coordinates):
+CC
+CC	  PS  chr  phaseSetStart  phaseSetEnd
+CC	  FL  chr  filterStart    filterEnd
+CC	  M?  chr  PS  pos  allele0  allele1  hetIndex  #supports0  #errors0  #supp1  #err1
+CC
+CC
+PS	chrI	1412	1542
+M1	chrI	1412	1412	T	C	1	7	0	9	0
+M1	chrI	1412	1414	G	A	2	7	0	9	0
+M1	chrI	1412	1421	G	A	3	7	0	9	0
+M1	chrI	1412	1471	T	C	4	5	0	6	0
+M1	chrI	1412	1542	C	T	5	3	0	2	0
+EV	0	chrI	1	40	3M	*	0	0	TGG	*	YP:i:0	YF:i:0	YI:i:3	YO:i:0	YS:i:1366
+EV	0	chrI	1	40	3M	*	0	0	CAA	*	YP:i:1	YF:i:0	YI:i:3	YO:i:0	YS:i:1343
+EV	0	chrI	1	40	4M	*	0	0	TGGT	*	YP:i:0	YF:i:0	YI:i:4	YO:i:0	YS:i:1409
+EV	0	chrI	1	40	3M	*	0	0	CAA	*	YP:i:1	YF:i:0	YI:i:3	YO:i:0	YS:i:1353
+EV	0	chrI	1	40	3M	*	0	0	TGG	*	YP:i:0	YF:i:0	YI:i:3	YO:i:0	YS:i:1358
+EV	0	chrI	1	40	4M	*	0	0	CAAC	*	YP:i:1	YF:i:0	YI:i:4	YO:i:0	YS:i:1374
+EV	0	chrI	1	40	3M	*	0	0	CAA	*	YP:i:1	YF:i:0	YI:i:3	YO:i:0	YS:i:1350
+EV	0	chrI	1	40	4M	*	0	0	CAAC	*	YP:i:1	YF:i:0	YI:i:4	YO:i:0	YS:i:1379
+EV	0	chrI	1	40	4M	*	0	0	CAAC	*	YP:i:1	YF:i:0	YI:i:4	YO:i:0	YS:i:1389
+EV	0	chrI	1	40	3M	*	0	0	TGG	*	YP:i:0	YF:i:0	YI:i:3	YO:i:0	YS:i:1341
+EV	0	chrI	1	40	3M	*	0	0	TGG	*	YP:i:0	YF:i:0	YI:i:3	YO:i:0	YS:i:1340
+EV	0	chrI	1	40	3M	*	0	0	CAA	*	YP:i:1	YF:i:0	YI:i:3	YO:i:0	YS:i:1359
+EV	0	chrI	1	40	4M	*	0	0	CAAC	*	YP:i:1	YF:i:0	YI:i:4	YO:i:0	YS:i:1392
+EV	0	chrI	1	40	4M	*	0	0	TGGT	*	YP:i:0	YF:i:0	YI:i:4	YO:i:0	YS:i:1407
+EV	0	chrI	1	40	3M	*	0	0	TGG	*	YP:i:0	YF:i:0	YI:i:3	YO:i:0	YS:i:1346
+EV	0	chrI	1	40	3M	*	0	0	CAA	*	YP:i:1	YF:i:0	YI:i:3	YO:i:0	YS:i:1348
+EV	0	chrI	4	40	2M	*	0	0	TC	*	YP:i:0	YF:i:0	YI:i:2	YO:i:0	YS:i:1444
+EV	0	chrI	4	40	2M	*	0	0	CT	*	YP:i:1	YF:i:0	YI:i:2	YO:i:0	YS:i:1462
+EV	0	chrI	4	40	2M	*	0	0	CT	*	YP:i:1	YF:i:0	YI:i:2	YO:i:0	YS:i:1457
+EV	0	chrI	4	40	2M	*	0	0	TC	*	YP:i:0	YF:i:0	YI:i:2	YO:i:0	YS:i:1468
+EV	0	chrI	4	40	2M	*	0	0	TC	*	YP:i:0	YF:i:0	YI:i:2	YO:i:0	YS:i:1461
+//
+PS	chrI	3611	3731
+M1	chrI	3611	3611	T	A	6	5	0	4	0
+M1	chrI	3611	3659	C	T	7	9	0	6	0
+M1	chrI	3611	3731	T	A	8	4	0	1	1
+EV	0	chrI	6	40	2M	*	0	0	TC	*	YP:i:0	YF:i:0	YI:i:2	YO:i:0	YS:i:3569
+EV	0	chrI	6	40	2M	*	0	0	TC	*	YP:i:0	YF:i:0	YI:i:2	YO:i:0	YS:i:3569
+EV	0	chrI	6	40	2M	*	0	0	AT	*	YP:i:1	YF:i:0	YI:i:2	YO:i:0	YS:i:3576
+EV	0	chrI	6	40	2M	*	0	0	TC	*	YP:i:0	YF:i:0	YI:i:2	YO:i:0	YS:i:3571
+EV	0	chrI	6	40	2M	*	0	0	AT	*	YP:i:1	YF:i:0	YI:i:2	YO:i:0	YS:i:3568
+EV	0	chrI	6	40	2M	*	0	0	AT	*	YP:i:1	YF:i:0	YI:i:2	YO:i:0	YS:i:3578
+EV	0	chrI	6	40	2M	*	0	0	AT	*	YP:i:1	YF:i:0	YI:i:2	YO:i:0	YS:i:3590
+EV	0	chrI	6	40	2M	*	0	0	TC	*	YP:i:0	YF:i:0	YI:i:2	YO:i:0	YS:i:3559
+EV	0	chrI	6	40	2M	*	0	0	TC	*	YP:i:0	YF:i:0	YI:i:2	YO:i:0	YS:i:3609
+EV	0	chrI	7	40	2M	*	0	0	TA	*	YP:i:1	YF:i:0	YI:i:2	YO:i:0	YS:i:3644
+EV	0	chrI	7	40	2M	*	0	0	CT	*	YP:i:0	YF:i:0	YI:i:2	YO:i:0	YS:i:3651
+EV	0	chrI	7	40	2M	*	0	0	CT	*	YP:i:0	YF:i:0	YI:i:2	YO:i:0	YS:i:3637
+EV	0	chrI	7	40	2M	*	0	0	CT	*	YP:i:0	YF:i:0	YI:i:2	YO:i:0	YS:i:3647
+EV	0	chrI	7	40	2M	*	0	0	TT	*	YP:i:1	YF:i:0	YI:i:1	YO:i:1	YS:i:3657
+EV	0	chrI	7	40	2M	*	0	0	CT	*	YP:i:0	YF:i:0	YI:i:2	YO:i:0	YS:i:3644
+//
diff --git a/tool_collections/samtools/samtools_phase/test-data/samtools_phase_out_3_phase0.bam b/tool_collections/samtools/samtools_phase/test-data/samtools_phase_out_3_phase0.bam
new file mode 100644
index 0000000000000000000000000000000000000000..1f1aa1a17f2ed848bfda8ace99064c3a0048c16a
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diff --git a/tool_collections/samtools/samtools_phase/tool_dependencies.xml b/tool_collections/samtools/samtools_phase/tool_dependencies.xml
new file mode 120000
index 00000000000..b11b378d63c
--- /dev/null
+++ b/tool_collections/samtools/samtools_phase/tool_dependencies.xml
@@ -0,0 +1 @@
+../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_reheader/.shed.yml b/tool_collections/samtools/samtools_reheader/.shed.yml
new file mode 100644
index 00000000000..4d7a5323f2c
--- /dev/null
+++ b/tool_collections/samtools/samtools_reheader/.shed.yml
@@ -0,0 +1,10 @@
+categories:
+- SAM
+description: Replace SAM header.
+long_description: |
+  Add or replace the header in a SAM or BAM file with a new header.
+name: samtools_reheader
+owner: devteam
+homepage_url: http://www.htslib.org/
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_reheader
+type: unrestricted
diff --git a/tool_collections/samtools/samtools_reheader/macros.xml b/tool_collections/samtools/samtools_reheader/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/samtools/samtools_reheader/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_reheader/samtools_reheader.xml b/tool_collections/samtools/samtools_reheader/samtools_reheader.xml
new file mode 100644
index 00000000000..ac8959dd371
--- /dev/null
+++ b/tool_collections/samtools/samtools_reheader/samtools_reheader.xml
@@ -0,0 +1,32 @@
+<tool id="samtools_reheader" name="Reheader" version="2.0">
+    <description>copy SAM/BAM header between datasets</description>
+    <macros>
+    <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"></expand>
+  <expand macro="stdio"></expand>
+  <expand macro="version_command"></expand>
+    <command><![CDATA[samtools reheader "${input_header}" "${input_file}" > "${output}"]]></command>
+    <inputs>
+        <param name="input_header" type="data" format="sam,bam" label="Select source dataset" help="Header from this dataset will be used to replace header in the target dataset. May be either SAM or BAM dataset."/>
+        <param name="input_file" type="data" format="bam" label="Select target dataset" help="Header from the source dataset will be inserted here. Should be a BAM dataset."/>
+    </inputs>
+    <outputs>
+        <data format="bam" name="output" label="${tool.name} on ${on_string}" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="input_header" ftype="bam" value="reheader_input_header.bam"/>
+            <param name="input_file" ftype="bam" value="reheader_input_file.bam"/>
+            <output name="output" ftype="bam" file="reheader_out.bam"/>
+        </test>
+    </tests>
+    <help>
+**What it does**
+
+Copies header from *source* dataset into *target* dataset using ``samtools reheader`` command.
+
+    </help>
+    <expand macro="citations"></expand>
+</tool>
+
diff --git a/tool_collections/samtools/samtools_reheader/test-data/reheader_input_file.bam b/tool_collections/samtools/samtools_reheader/test-data/reheader_input_file.bam
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diff --git a/tool_collections/samtools/samtools_reheader/test-data/reheader_input_header.bam b/tool_collections/samtools/samtools_reheader/test-data/reheader_input_header.bam
new file mode 100644
index 0000000000000000000000000000000000000000..5522416c11aff28490121d95c9f65fe904e23b13
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diff --git a/tool_collections/samtools/samtools_reheader/test-data/reheader_out.bam b/tool_collections/samtools/samtools_reheader/test-data/reheader_out.bam
new file mode 100644
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diff --git a/tool_collections/samtools/samtools_reheader/tool_dependencies.xml b/tool_collections/samtools/samtools_reheader/tool_dependencies.xml
new file mode 120000
index 00000000000..b11b378d63c
--- /dev/null
+++ b/tool_collections/samtools/samtools_reheader/tool_dependencies.xml
@@ -0,0 +1 @@
+../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_rmdup/.shed.yml b/tool_collections/samtools/samtools_rmdup/.shed.yml
new file mode 100644
index 00000000000..90dd377e794
--- /dev/null
+++ b/tool_collections/samtools/samtools_rmdup/.shed.yml
@@ -0,0 +1,14 @@
+categories:
+- SAM
+description: Remove PCR duplicates
+long_description: |
+  This tool uses the SAMtools toolkit to remove potential PCR duplicates:
+  if multiple read pairs have identical external coordinates, only retain the pair
+  with highest mapping quality. In the paired-end mode, this command ONLY works with
+  FR orientation and requires ISIZE is correctly set. It does not work for unpaired
+  reads (e.g. two ends mapped to different chromosomes or orphan reads).
+name: samtools_rmdup
+owner: devteam
+homepage_url: http://www.htslib.org/
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_rmdup
+type: unrestricted
diff --git a/tool_collections/samtools/samtools_rmdup/macros.xml b/tool_collections/samtools/samtools_rmdup/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/samtools/samtools_rmdup/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_rmdup/samtools_rmdup.xml b/tool_collections/samtools/samtools_rmdup/samtools_rmdup.xml
new file mode 100644
index 00000000000..dae1fce3976
--- /dev/null
+++ b/tool_collections/samtools/samtools_rmdup/samtools_rmdup.xml
@@ -0,0 +1,56 @@
+<tool id="samtools_rmdup" name="RmDup" version="2.0">
+  <description>remove PCR duplicates</description>
+  <macros>
+    <import>macros.xml</import>
+  </macros>
+  <requirements>
+    <requirement type="package" version="0.1.19">samtools</requirement>
+  </requirements>
+  <expand macro="stdio"></expand>
+  <expand macro="version_command"></expand>
+  <command>samtools rmdup 
+  #if str( $bam_paired_end_type.bam_paired_end_type_selector ) == "PE"
+      ${bam_paired_end_type.force_se}
+  #else:
+      -s
+  #end if
+  "$input1" "$output1"
+  </command>
+  <inputs>
+    <param name="input1" type="data" format="bam" label="BAM File" />
+    
+    <conditional name="bam_paired_end_type">
+      <param name="bam_paired_end_type_selector" type="select" label="Is this paired-end or single end data">
+        <option value="PE" selected="True">BAM is paired-end</option>
+        <option value="SE">BAM is single-end (-s)</option>
+      </param>
+      <when value="PE">
+        <param name="force_se" type="boolean" label="Treat as single-end" help="-S" truevalue="-S" falsevalue="" checked="False"/>
+      </when>
+      <when value="SE" /> <!-- No extra parameters here -->
+    </conditional>
+    
+  </inputs>
+  <outputs>
+    <data name="output1" format="bam" />
+  </outputs>
+  <tests>
+    <test>
+      <param name="input1" value="samtools-rmdup-input1.bam" ftype="bam" />
+      <param name="bam_paired_end_type_selector" value="PE" />
+      <param name="force_se" />
+      <output name="output1" file="samtools-rmdup-test1.bam" ftype="bam" sort="True" />
+    </test>
+  </tests>
+  <help>
+
+**What it does**
+
+Remove potential PCR duplicates: if multiple read pairs have identical external coordinates, only retain the pair with highest mapping quality. In the paired-end mode, this command ONLY works with FR orientation and requires ISIZE is correctly set. It does not work for unpaired reads (e.g. two ends mapped to different chromosomes or orphan reads). This tool has the following parameters::
+
+  -s    rmdup for SE reads
+  -S    treat PE reads as SE in rmdup (force -s)
+
+  </help>
+    <expand macro="citations"></expand>
+</tool>
diff --git a/tool_collections/samtools/samtools_rmdup/test-data/samtools-rmdup-input1.bam b/tool_collections/samtools/samtools_rmdup/test-data/samtools-rmdup-input1.bam
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diff --git a/tool_collections/samtools/samtools_rmdup/test-data/samtools-rmdup-test1.bam b/tool_collections/samtools/samtools_rmdup/test-data/samtools-rmdup-test1.bam
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diff --git a/tool_collections/samtools/samtools_rmdup/tool_dependencies.xml b/tool_collections/samtools/samtools_rmdup/tool_dependencies.xml
new file mode 100644
index 00000000000..03998a77628
--- /dev/null
+++ b/tool_collections/samtools/samtools_rmdup/tool_dependencies.xml
@@ -0,0 +1,6 @@
+<?xml version="1.0"?>
+<tool_dependency>
+    <package name="samtools" version="0.1.19">
+        <repository name="package_samtools_0_1_19" owner="iuc" />
+    </package>
+</tool_dependency>
diff --git a/tool_collections/samtools/samtools_slice_bam/.shed.yml b/tool_collections/samtools/samtools_slice_bam/.shed.yml
new file mode 100644
index 00000000000..9b7fa53e074
--- /dev/null
+++ b/tool_collections/samtools/samtools_slice_bam/.shed.yml
@@ -0,0 +1,11 @@
+categories:
+- SAM
+description: Slice BAM by provided regions
+long_description: |
+  Accepts an input BAM file and an input BED file and creates an
+  output BAM file containing only those alignments that overlap the provided BED intervals.
+name: samtools_slice_bam
+owner: devteam
+homepage_url: http://www.htslib.org/
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_slice_bam
+type: unrestricted
diff --git a/tool_collections/samtools/samtools_slice_bam/macros.xml b/tool_collections/samtools/samtools_slice_bam/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/samtools/samtools_slice_bam/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_slice_bam/samtools_slice_bam.xml b/tool_collections/samtools/samtools_slice_bam/samtools_slice_bam.xml
new file mode 100644
index 00000000000..c95c19b74c8
--- /dev/null
+++ b/tool_collections/samtools/samtools_slice_bam/samtools_slice_bam.xml
@@ -0,0 +1,123 @@
+<tool id="samtools_slice_bam" name="Slice" version="2.0">
+  <description>BAM by genomic regions</description>
+  <macros>
+    <import>macros.xml</import>
+  </macros>
+  <!-- <code file="samtools_slice_options.py"/> -->
+  <expand macro="requirements"></expand>
+  <expand macro="stdio"></expand>
+  <expand macro="version_command"></expand>
+    <command>
+<![CDATA[
+    ln -s "${input_bam}" temp_input.bam &&
+    ln -s "${input_bam.metadata.bam_index}" temp_input.bam.bai &&
+
+    #if str($slice_method.slice_method_selector) == "bed":
+    
+        samtools view -@ \${GALAXY_SLOTS:-1} -b -L "${input_interval}" -o unsorted_output.bam temp_input.bam && 
+
+    #elif str($slice_method.slice_method_selector) == "chr":
+
+        samtools view -@ \${GALAXY_SLOTS:-1} -b -o unsorted_output.bam temp_input.bam 
+        ${ ' '.join( map( lambda x:'"%s"' % ( x ), str( $slice_method.refs ).split(",") ) ) } &&
+
+    #elif str($slice_method.slice_method_selector) == "man":
+
+        samtools view -@ \${GALAXY_SLOTS:-1} -b -o unsorted_output.bam temp_input.bam 
+
+        #for $region in $slice_method.regions:
+            "${region.chrom}:${region.start}-${region.end}"
+        #end for
+
+        &&
+
+    #end if
+
+    samtools sort -O bam -T sorted -@ \${GALAXY_SLOTS:-1} -o "${output_bam}" unsorted_output.bam
+]]>
+    </command>
+    <inputs>
+        <param name="input_bam" format="bam" label="Select BAM dataset to slice" type="data" />
+        <conditional name="slice_method">
+            <param name="slice_method_selector" type="select" label="How do you want to slice your dataset?">
+                <option value="bed">using a list of intervals from a BED dataset</option>
+                <option value="chr">by chromosomes/contigs present in the BAM dataset</option>
+                <option value="man">by chromosomes/contigs and coordinates</option>
+            </param>
+            <when value="bed">
+                <param format="bed" label="BED file" name="input_interval" type="data" help="BED datasets can be obtained using &quot;Get Data -> UCSC Main&quot; datasource."/>
+            </when>
+            <when value="chr">
+                <param name="refs" type="select" optional="False" multiple="True" label="Select references (chromosomes and contigs) you would like to restrict bam to" help="Click and type in the box above to see options. You can select multiple entries. If &quot;No options available&quot; is displayed, you need to re-detect metadata on the input dataset. See help section below.">
+
+                    <!-- The options tagset below extracts reference names from bam file metadata -->
+                    <!-- This will not work with bed files with old style metadata. However this  -->
+                    <!-- Can be easily fixed by re-deceting metadata on a bam dataset by clicking -->
+                    <!-- The pencil icon and settind datatype to "bam"                            -->
+                    <!-- This change has been commited in the following pull request:             -->
+                    <!-- https://github.com/galaxyproject/galaxy/pull/107                         -->
+
+                    <options>
+                        <filter type="data_meta" ref="input_bam" key="reference_names" />
+                    </options>
+                </param>
+            </when>
+            <when value="man">
+                <repeat name="regions" title="Regions" min="1">
+                    <param name="chrom" type="select" optional="False" label="Select references (chromosomes and contigs) you would like to restrict bam to" help="Select chromosome/contig from the list. If &quot;No options available&quot; is displayed, you need to re-detect metadata on the input dataset. See help section below.">
+
+                        <!-- See comments above -->
+
+                        <options>
+                            <filter type="data_meta" ref="input_bam" key="reference_names" />
+                        </options>
+                    </param>
+                    <param name="start" type="integer" min="1" value="0" label="Enter START coordinate (1-based)"/>
+                    <param name="end" type="integer" min="1" value="100" label="Enter END coordinate"/>
+                </repeat>
+
+
+            </when>
+        </conditional>
+
+    </inputs>
+    <outputs>
+        <data format="bam" name="output_bam" />
+    </outputs>
+    <tests>
+        <test>
+            <param ftype="bam" name="input_bam" value="bam-slice-input.bam" />
+            <param name="slice_method_selector" value="bed"/>
+            <param ftype="bed" name="input_interval" value="bam-slice.bed" />
+            <output file="bam-slice-test1.bam" ftype="bam" name="output_bam" />
+        </test>
+        <test>
+            <param ftype="bam" name="input_bam" value="bam-slice-input.bam" />
+            <param name="slice_method_selector" value="chr"/>
+            <param name="refs" value="chrM" />
+            <output file="bam-slice-test2.bam" ftype="bam" name="output_bam" />
+        </test>
+        <test>
+            <param ftype="bam" name="input_bam" value="bam-slice-input.bam" />
+            <param name="slice_method_selector" value="man"/>
+            <param name="chrom" value="chrM" />
+            <param name="start" value="1" />
+            <param name="end" value="1000" />
+            <output file="bam-slice-test3.bam" ftype="bam" name="output_bam" />
+        </test>
+    </tests>
+    <help>
+<![CDATA[
+
+**What it does**
+
+Allows to restrict (slice) input BAM dataset to a list of intervals defined in a BED file, individual chromosomes, or manually set list of coordinates. BED datasets can be obtained from **Get Data -> UCSC Main**.
+
+This tool is based on ``samtools view`` command. 
+
+@no-chrom-options@
+
+]]>
+  </help>
+    <expand macro="citations"></expand>
+</tool>
diff --git a/tool_collections/samtools/samtools_slice_bam/test-data/bam-slice-input.bam b/tool_collections/samtools/samtools_slice_bam/test-data/bam-slice-input.bam
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diff --git a/tool_collections/samtools/samtools_slice_bam/test-data/bam-slice-test1.bam b/tool_collections/samtools/samtools_slice_bam/test-data/bam-slice-test1.bam
new file mode 100644
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diff --git a/tool_collections/samtools/samtools_slice_bam/test-data/bam-slice-test2.bam b/tool_collections/samtools/samtools_slice_bam/test-data/bam-slice-test2.bam
new file mode 100644
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diff --git a/tool_collections/samtools/samtools_slice_bam/test-data/bam-slice.bed b/tool_collections/samtools/samtools_slice_bam/test-data/bam-slice.bed
new file mode 100644
index 00000000000..3dc32e5ce49
--- /dev/null
+++ b/tool_collections/samtools/samtools_slice_bam/test-data/bam-slice.bed
@@ -0,0 +1,38 @@
+chrM	5	1000	myInterval	0	+
+chrM	577	647	TRNF	0	+
+chrM	648	1601	RNR1	0	+
+chrM	1602	1670	TRNV	0	+
+chrM	1671	3229	RNR2	0	+
+chrM	3230	3304	TRNL1	0	+
+chrM	3307	4262	ND1	0	+
+chrM	4263	4331	TRNI	0	+
+chrM	4329	4400	TRNQ	0	-
+chrM	4402	4469	TRNM	0	+
+chrM	4470	5511	ND2	0	+
+chrM	5512	5579	TRNW	0	+
+chrM	5587	5655	TRNA	0	-
+chrM	5657	5729	TRNN	0	-
+chrM	5761	5826	TRNC	0	-
+chrM	5826	5891	TRNY	0	-
+chrM	5904	7445	COX1	0	+
+chrM	7446	7514	TRNS1	0	-
+chrM	7518	7585	TRND	0	+
+chrM	7586	8269	COX2	0	+
+chrM	8295	8364	TRNK	0	+
+chrM	8366	8572	ATP8	0	+
+chrM	8527	9207	ATP6	0	+
+chrM	9207	9990	COX3	0	+
+chrM	9991	10058	TRNG	0	+
+chrM	10059	10404	ND3	0	+
+chrM	10405	10469	TRNR	0	+
+chrM	10470	10766	ND4L	0	+
+chrM	10760	12137	ND4	0	+
+chrM	12138	12206	TRNH	0	+
+chrM	12207	12265	TRNS2	0	+
+chrM	12266	12336	TRNL2	0	+
+chrM	12337	14148	ND5	0	+
+chrM	14149	14673	ND6	0	-
+chrM	14674	14742	TRNE	0	-
+chrM	14747	15887	CYTB	0	+
+chrM	15888	15953	TRNT	0	+
+chrM	15956	16023	TRNP	0	-
diff --git a/tool_collections/samtools/samtools_slice_bam/tool_dependencies.xml b/tool_collections/samtools/samtools_slice_bam/tool_dependencies.xml
new file mode 120000
index 00000000000..b11b378d63c
--- /dev/null
+++ b/tool_collections/samtools/samtools_slice_bam/tool_dependencies.xml
@@ -0,0 +1 @@
+../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_sort/.shed.yml b/tool_collections/samtools/samtools_sort/.shed.yml
new file mode 100644
index 00000000000..1ad7582aae7
--- /dev/null
+++ b/tool_collections/samtools/samtools_sort/.shed.yml
@@ -0,0 +1,11 @@
+categories:
+- SAM
+description: Sort alignments by leftmost coordinates or read name.
+long_description: |
+  This tool uses the SAMtools toolkit to sort alignments by leftmost
+  coordinates or read names.
+name: samtools_sort
+owner: devteam
+homepage_url: http://www.htslib.org/
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_sort
+type: unrestricted
diff --git a/tool_collections/samtools/samtools_sort/macros.xml b/tool_collections/samtools/samtools_sort/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/samtools/samtools_sort/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_sort/samtools_sort.xml b/tool_collections/samtools/samtools_sort/samtools_sort.xml
new file mode 100644
index 00000000000..96b2ad857db
--- /dev/null
+++ b/tool_collections/samtools/samtools_sort/samtools_sort.xml
@@ -0,0 +1,40 @@
+<tool id="samtools_sort" name="Sort" version="2.0">
+    <description>BAM dataset</description>
+    <macros>
+    <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"></expand>
+    <expand macro="stdio"></expand>
+    <expand macro="version_command"></expand>
+    <command>samtools sort $sort_mode -@ \${GALAXY_SLOTS:-1} -o "${output1}" -O bam -T dataset "${input1}"</command>
+    <inputs>
+        <param name="input1" type="data" format="bam" label="BAM File" />
+        <param name="sort_mode" type="select" label="Sort by ">
+            <option value="" selected="True">Chromosomal coordinates</option>
+            <option value="-n">Read names (-n)</option>
+        </param>
+    </inputs>
+    <outputs>
+        <data name="output1" format="bam" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="input1" value="1.bam" ftype="bam" />
+            <output name="output1" file="1_sort.bam" ftype="bam" sort="True"/>
+        </test>
+        <test>
+            <param name="input1" value="1.bam" ftype="bam" />
+            <param name="sort_mode" value="-n"/>
+            <output name="output1" file="1_sort_read_names.bam" ftype="bam" sort="True"/>
+        </test>
+    </tests>
+    <help>
+
+**What it does**
+
+This tool uses ``samtools sort`` command to sort BAM datasets in coordinate or read name order.
+
+
+    </help>
+    <expand macro="citations"></expand>
+</tool>
diff --git a/tool_collections/samtools/samtools_sort/test-data/1.bam b/tool_collections/samtools/samtools_sort/test-data/1.bam
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diff --git a/tool_collections/samtools/samtools_sort/tool_dependencies.xml b/tool_collections/samtools/samtools_sort/tool_dependencies.xml
new file mode 120000
index 00000000000..b11b378d63c
--- /dev/null
+++ b/tool_collections/samtools/samtools_sort/tool_dependencies.xml
@@ -0,0 +1 @@
+../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_split/.shed.yml b/tool_collections/samtools/samtools_split/.shed.yml
new file mode 100644
index 00000000000..67d0210b75e
--- /dev/null
+++ b/tool_collections/samtools/samtools_split/.shed.yml
@@ -0,0 +1,11 @@
+categories:
+- SAM
+description: 'Split a BAM file by read group'
+long_description: |
+  This tool runs the samtools split command in the SAMtools toolkit
+  to split BAM files by read group.
+name: samtools_split
+owner: devteam
+homepage_url: http://www.htslib.org/
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_split
+type: unrestricted
diff --git a/tool_collections/samtools/samtools_split/macros.xml b/tool_collections/samtools/samtools_split/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/samtools/samtools_split/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_split/samtools_split.xml b/tool_collections/samtools/samtools_split/samtools_split.xml
new file mode 100644
index 00000000000..bfd2e884970
--- /dev/null
+++ b/tool_collections/samtools/samtools_split/samtools_split.xml
@@ -0,0 +1,47 @@
+<tool id="samtools_split" name="Split" version="1.1">
+    <description>BAM dataset on readgroups</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"></expand>
+    <expand macro="stdio"></expand>
+    <expand macro="version_command"></expand>
+    <command><![CDATA[
+        samtools split -f 'Read_Group_%!.bam'
+        #if $header:
+            -u "${output}":"${header}"
+        #else:
+            -u "${output}"
+        #end if
+        "${input_bam}"
+        ]]></command>
+    <inputs>
+        <param name="input_bam" type="data" format="bam" label="BAM file" />
+        <param name="header" type="data" format="bam,sam" label="Replace header in output file" optional="True" />
+     </inputs>
+    <outputs>
+        <data format="bam" name="output" label="${tool.name} on ${on_string}">
+            <discover_datasets pattern="Read_Group_(?P&lt;designation&gt;.+)\.bam" ext="bam" visible="true" directory="" />
+        </data>
+    </outputs>
+    <tests>
+        <test>
+            <param name="input_bam" value="phiX.bam" ftype="bam" />
+            <output name="output" file="samtools_split_out.bam">
+                <discovered_dataset designation="Mouse" ftype="bam" file="phiX_Mouse.bam" />
+                <discovered_dataset designation="Elephant" ftype="bam" file="phiX_Elephant.bam" />
+                <discovered_dataset designation="Human" ftype="bam" file="phiX_Human.bam" />
+            </output>
+        </test>
+    </tests>
+    <help>
+**What it does**
+
+Splits BAM files on readgroups.
+
+This tool is based on ``samtools split`` command. It will generate multiple output datasets for each redagroup from the input dataset. 
+
+    </help>
+    <expand macro="citations"></expand>
+</tool>
+
diff --git a/tool_collections/samtools/samtools_split/test-data/phiX.bam b/tool_collections/samtools/samtools_split/test-data/phiX.bam
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diff --git a/tool_collections/samtools/samtools_split/test-data/phiX_Human.bam b/tool_collections/samtools/samtools_split/test-data/phiX_Human.bam
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diff --git a/tool_collections/samtools/samtools_split/test-data/phiX_Mouse.bam b/tool_collections/samtools/samtools_split/test-data/phiX_Mouse.bam
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diff --git a/tool_collections/samtools/samtools_split/test-data/samtools_split_out.bam b/tool_collections/samtools/samtools_split/test-data/samtools_split_out.bam
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diff --git a/tool_collections/samtools/samtools_split/tool_dependencies.xml b/tool_collections/samtools/samtools_split/tool_dependencies.xml
new file mode 120000
index 00000000000..b11b378d63c
--- /dev/null
+++ b/tool_collections/samtools/samtools_split/tool_dependencies.xml
@@ -0,0 +1 @@
+../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_stats/.shed.yml b/tool_collections/samtools/samtools_stats/.shed.yml
new file mode 100644
index 00000000000..200ac5f35ee
--- /dev/null
+++ b/tool_collections/samtools/samtools_stats/.shed.yml
@@ -0,0 +1,12 @@
+categories:
+- SAM
+- Statistics
+description: Generate statistics for a BAM or SAM file
+long_description: |
+  This tool runs the samtools stats command in the SAMtools toolkit,
+  collecting statistics from BAM files. The output can be visualized using plot-bamstats.
+name: samtools_stats
+owner: devteam
+homepage_url: http://www.htslib.org/
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_stats
+type: unrestricted
diff --git a/tool_collections/samtools/samtools_stats/macros.xml b/tool_collections/samtools/samtools_stats/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/samtools/samtools_stats/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_stats/samtools_stats.xml b/tool_collections/samtools/samtools_stats/samtools_stats.xml
new file mode 100644
index 00000000000..ab360820353
--- /dev/null
+++ b/tool_collections/samtools/samtools_stats/samtools_stats.xml
@@ -0,0 +1,296 @@
+<tool id="samtools_stats" name="Stats" version="2.0">
+    <description>generate statistics for BAM dataset</description>
+    <macros>
+    <import>macros.xml</import>
+  </macros>
+    <expand macro="requirements"></expand>
+    <expand macro="stdio"></expand>
+    <expand macro="version_command"></expand>
+    <command><![CDATA[
+        #if $use_reference.use_ref_selector == "yes":
+            #if $use_reference.reference_source.reference_source_selector == "history":
+               ln -s "${use_reference.reference_source.ref_file}" && samtools faidx `basename "${use_reference.reference_source.ref_file}"` && samtools stats
+            #else:
+                samtools stats
+            #end if
+        #else:
+            samtools stats
+        #end if
+        "${input_file}"
+        --coverage ${coverage_min},${coverage_max},${coverage_step}
+        ${remove_dups}
+        #if str( $filter_by_flags.filter_flags ) == "filter":
+            #if $filter_by_flags.require_flags:
+                --required-flag ${sum([int(flag) for flag in str($filter_by_flags.require_flags).split(',')])}
+            #end if
+            #if $filter_by_flags.exclude_flags:
+                --filtering-flag ${sum([int(flag) for flag in str($filter_by_flags.exclude_flags).split(',')])}
+            #end if
+        #end if
+        --GC-depth ${gc_depth}
+        --insert-size ${insert_size}
+
+        ## The code below is commented out because using -I/--id options causes the following exception
+        ## Samtools-htslib: init_group_id() header parsing not yet implemented
+
+        ##if str($read_group) != "":
+        ##    -I "${read_group}"
+        ##end if
+
+        #if str($read_length) != "0":
+            --read-length "${read_length}"
+        #end if
+        --most-inserts ${most_inserts}
+        --trim-quality ${trim_quality}
+        #if $use_reference.use_ref_selector == "yes":
+            #if $use_reference.reference_source.reference_source_selector != "history":
+                --ref-seq "${use_reference.reference_source.ref_file.fields.path}"
+            #else:
+                --ref-seq "${use_reference.reference_source.ref_file}"
+            #end if
+        #end if
+        > "${output}"
+        #if $split_output.split_output_selector == "yes":
+            #set outputs_to_split = str($split_output.generate_tables).split(',')
+            && mkdir split && echo ${split_output.generate_tables} &&
+
+            #if 'sn' in $outputs_to_split:
+                echo "# Summary Numbers\n"  > "split/Summary numbers.tab" &&
+                grep -q ^SN  "${output}" ; if [ $? = 0 ] ; then grep ^SN  "${output}" | cut -f 2- >> "split/Summary numbers.tab"  ; fi &&
+            #end if
+
+            #if 'ffq' in $outputs_to_split:
+                echo "# Columns correspond to qualities and rows to cycles. First column is the cycle number\n" > "split/First Fragment Qualities.tab" &&
+                grep -q ^FFQ "${output}" ; if [ $? = 0 ] ; then grep ^FFQ "${output}" | cut -f 2- >> "split/First Fragment Qualities.tab" ; fi &&
+            #end if
+
+            #if 'lfq' in $outputs_to_split:
+                echo "# Columns correspond to qualities and rows to cycles. First column is the cycle number" > "split/Last Fragment Qualities.tab" &&
+                grep -q ^LFQ "${output}" ; if [ $? = 0 ] ; then grep ^LFQ "${output}" | cut -f 2- >> "split/Last Fragment Qualities.tab" ; fi &&
+            #end if
+
+            #if 'mpc' in $outputs_to_split:
+                echo "# Columns correspond to qualities, rows to cycles. First column is the cycle number, second is the number of N's and the rest is the number of mismatches" > "split/Mismatches per cycle.tab" &&
+                grep -q ^MPC "${output}" ; if [ $? = 0 ] ; then grep ^MPC "${output}" | cut -f 2- >> "split/Mismatches per cycle.tab" ; fi &&
+            #end if
+
+            #if 'gcf' in $outputs_to_split:
+                echo "# GC Content of first fragments" > "split/GC Content of first fragments.tab" &&
+                grep -q ^GCF "${output}" ; if [ $? = 0 ] ; then grep ^GCF "${output}" | cut -f 2- >> "split/GC Content of first fragments.tab" ; fi &&
+            #end if
+
+            #if 'gcl' in $outputs_to_split:
+                echo "# GC Content of last fragments" > "split/GC Content of last fragments.tab" &&
+                grep -q ^GCL "${output}" ; if [ $? = 0 ] ; then grep ^GCL "${output}" | cut -f 2- >> "split/GC Content of last fragments.tab" ; fi &&
+            #end if
+
+            #if 'gcc' in $outputs_to_split:
+                echo "# ACGT content per cycle. The columns are: cycle, and A,C,G,T counts (percent)" > "split/ACGT content per cycle.tab" &&
+                grep -q ^GCC "${output}" ; if [ $? = 0 ] ; then grep ^GCC "${output}" | cut -f 2- >> "split/ACGT content per cycle.tab" ; fi &&
+            #end if
+
+            #if 'is' in $outputs_to_split:
+                echo "# Insert sizes. The columns are: insert size, pairs total, inward oriented pairs, outward oriented pairs, other pairs" > "split/Insert sizes.tab" &&
+                grep -q ^IS  "${output}" ; if [ $? = 0 ] ; then grep ^IS  "${output}" | cut -f 2- >> "split/Insert sizes.tab"  ; fi &&
+            #end if
+
+            #if 'rl' in $outputs_to_split:
+                echo "# Read lengths. The columns are: read length, count" > "split/Read lengths.tab" &&
+                grep -q ^RL  "${output}" ; if [ $? = 0 ] ; then grep ^RL  "${output}" | cut -f 2- >> "split/Read lengths.tab"  ; fi &&
+            #end if
+
+            #if 'id' in $outputs_to_split:
+                echo "# Indel distribution. The columns are: length, number of insertions, number of deletions" > "split/Indel distribution.tab" &&
+                grep -q ^ID  "${output}" ; if [ $? = 0 ] ; then grep ^ID  "${output}" | cut -f 2- >> "split/Indel distribution.tab"  ; fi &&
+            #end if
+
+            #if 'ic' in $outputs_to_split:
+                echo "# Indels per cycle. The columns are: cycle, number of insertions (fwd), .. (rev) , number of deletions (fwd), .. (rev)" >  "split/Indels per cycle.tab"  &&
+                grep -q ^IC  "${output}" ; if [ $? = 0 ] ; then grep ^IC  "${output}" | cut -f 2- >> "split/Indels per cycle.tab"  ; fi &&
+            #end if
+
+            #if 'cov' in $outputs_to_split:
+                echo "# Coverage distribution" > "split/Coverage distribution.tab" &&
+                grep -q ^COV "${output}" ; if [ $? = 0 ] ; then grep ^COV "${output}" | cut -f 2- >> "split/Coverage distribution.tab" ; fi &&
+            #end if
+
+            #if 'gcd' in $outputs_to_split:
+                echo "# GC-depth. The columns are: GC%, unique sequence percentiles, 10th, 25th, 50th, 75th and 90th depth percentile" > "split/GC depth.tab" &&
+                grep -q ^GCD "${output}" ; if [ $? = 0 ] ; then grep ^GCD "${output}" | cut -f 2- >> "split/GC depth.tab" ; fi &&
+            #end if
+
+            ## Unix true command below
+
+            true
+
+        #end if
+        ]]></command>
+    <inputs>
+        <param name="input_file" type="data" format="sam,bam" label="BAM file" />
+        <param name="coverage_min" type="integer" value="1" label="Minimum coverage" help="minimum coverage value for --coverage option"/>
+        <param name="coverage_max" type="integer" value="1000" label="Maximum coverage" help="maximum coverage value for --coverage option"/>
+        <param name="coverage_step" type="integer" value="1" label="Coverage step" help="step value for --coverage option"/>
+        <param name="remove_dups" type="boolean" truevalue="--remove-dups" falsevalue="" checked="False" label="Exclude reads marked as duplicates" help="--remove-dups; default = False"/>
+        <conditional name="split_output">
+            <param name="split_output_selector" type="select" label="Output" help="Select between a single output or separate outputs for each statistics">
+                <option value="no" selected="True">a single summary file</option>
+                <option value="yes">separate datasets for each statistics</option>
+            </param>
+            <when value="no" />
+            <when value="yes">
+                <param name="generate_tables" type="select" display="checkboxes" multiple="True" label="Statistics to extract">
+                    <option value="sn">Summary numbers</option>
+                    <option value="ffq">First Fragment Qualities</option>
+                    <option value="lfq">Last Fragment Qualities</option>
+                    <option value="mpc">Mismatches per cycle</option>
+                    <option value="gcf">GC Content of first fragments</option>
+                    <option value="gcl">GC Content of last fragments</option>
+                    <option value="gcc">ACGT content per cycle</option>
+                    <option value="is">Insert sizes</option>
+                    <option value="rl">Read lengths</option>
+                    <option value="id">Indel distribution</option>
+                    <option value="ic">Indels per cycle</option>
+                    <option value="cov">Coverage distribution</option>
+                    <option value="gcd">GC depth</option>
+                </param>
+            </when>
+        </conditional>
+        <conditional name="filter_by_flags">
+            <param name="filter_flags" type="select" label="Set filter by flags" help="-f and -F options">
+                <option value="nofilter" selected="True">Do not filter</option>
+                <option value="filter">Filter by flags to exclude or require</option>
+            </param>
+            <when value="filter">
+                <param name="require_flags" type="select" display="checkboxes" multiple="True" label="Require" help="-f">
+                    <option value="1">Read is paired</option>
+                    <option value="2">Read is mapped in a proper pair</option>
+                    <option value="4">The read is unmapped</option>
+                    <option value="8">The mate is unmapped</option>
+                    <option value="16">Read strand</option>
+                    <option value="32">Mate strand</option>
+                    <option value="64">Read is the first in a pair</option>
+                    <option value="128">Read is the second in a pair</option>
+                    <option value="256">The alignment or this read is not primary</option>
+                    <option value="512">The read fails platform/vendor quality checks</option>
+                    <option value="1024">The read is a PCR or optical duplicate</option>
+                </param>
+                <param name="exclude_flags" type="select" display="checkboxes" multiple="True" label="Exclude" help="-F">
+                    <option value="1">Read is paired</option>
+                    <option value="2">Read is mapped in a proper pair</option>
+                    <option value="4">The read is unmapped</option>
+                    <option value="8">The mate is unmapped</option>
+                    <option value="16">Read strand</option>
+                    <option value="32">Mate strand</option>
+                    <option value="64">Read is the first in a pair</option>
+                    <option value="128">Read is the second in a pair</option>
+                    <option value="256">The alignment or this read is not primary</option>
+                    <option value="512">The read fails platform/vendor quality checks</option>
+                    <option value="1024">The read is a PCR or optical duplicate</option>
+                </param>
+            </when>
+            <when value="nofilter" />
+
+        </conditional>
+        <param name="gc_depth" type="float" value="20000" label="GC-depth bin size" help="--GC-depth; decreasing bin size increases memory requirement; default = 20000.0"/>
+        <param name="insert_size" type="integer" value="8000" label="Maximum insert size" help="--insert-size; default = 8000"/>
+
+        <!--
+            
+            The -I option of samtools stats returns the following message in version 1.2:
+
+            Samtools-htslib: init_group_id() header parsing not yet implemented
+            Abort trap: 6
+    
+            Because of this the section below is commented out until this stats bug is fixed
+
+            <param name="read_group" type="select" optional="true" label="Limit to a specific read group name" > 
+                <options>
+                    <filter type="data_meta" ref="input_file" key="read_groups" />
+                </options>
+            </param>
+
+        -->
+
+        <param name="read_length" type="integer" value="0" label="Minimum read length to generate statistics for" help="--read-length; default = no cutoff"/>
+        <param name="most_inserts" type="float" value="0.99" label="Report only the main part of inserts" help="--most-inserts; default = 0.99"/>
+        <param name="trim_quality" type="integer" value="0" label="BWA trim parameter" help="--trim-quality; default = 0"/>
+        
+        <conditional name="use_reference">
+            <param name="use_ref_selector" type="select" label="Use reference sequence" help="--ref-seq; required for GC-depth and mismatches-per-cycle calculation">
+                <option value="yes">Use reference</option>
+                <option selected="True" value="no">Do not use reference</option>
+            </param>
+            <when value="yes">
+                <conditional name="reference_source">
+                    <param name="reference_source_selector" type="select" label="Choose a reference sequence for GC depth">
+                        <option value="cached">Locally cached</option>
+                        <option value="history">History</option>
+                    </param>
+                    <when value="cached">
+                        <param name="ref_file" type="select" label="Using genome">
+                            <options from_data_table="fasta_indexes" />
+                            <filter type="data_meta" ref="input_file" key="dbkey" column="1" />
+                        </param>
+                    </when>
+                    <when value="history">
+                        <param name="ref_file" type="data" format="fasta" label="Using file" />
+                    </when>
+                </conditional>
+            </when>
+            <when value="no" />
+        </conditional>
+
+    </inputs>
+
+    <outputs>
+        <data format="tabular" name="output" label="${tool.name} on ${on_string}">
+            <discover_datasets pattern="(?P&lt;designation&gt;.+)\.tab" ext="tabular" visible="true" directory="split" />
+        </data>
+    </outputs>
+    <tests>
+        <test>
+            <param name="input_file" value="samtools_stats_input.bam" ftype="bam" />
+            <param name="use_ref_selector" value="yes" />
+            <param name="reference_source_selector" value="history" />
+            <param name="ref_file" value="samtools_stats_ref.fa" ftype="fasta" />
+            <output name="output" file="samtools_stats_out1.tab" ftype="tabular" lines_diff="4" />
+        </test>
+        <test>
+            <param name="input_file" value="samtools_stats_input.bam" ftype="bam" />
+            <param name="use_ref_selector" value="yes" />
+            <param name="reference_source_selector" value="history" />
+            <param name="ref_file" value="samtools_stats_ref.fa" ftype="fasta" />
+            <param name="split_output_selector" value="yes" />
+            <param name="generate_tables" value="sn,mpc,gcc" />
+            <output name="output" file="samtools_stats_out2.tab" lines_diff="4">
+                <discovered_dataset designation="Summary numbers" ftype="tabular" file="samtools_stats_out2/sn.tab" />
+                <discovered_dataset designation="ACGT content per cycle" ftype="tabular" file="samtools_stats_out2/gcc.tab" />
+                <discovered_dataset designation="Mismatches per cycle" ftype="tabular" file="samtools_stats_out2/mpc.tab" />
+            </output>
+        </test>
+    </tests>
+    <help><![CDATA[
+**What it does**
+
+This tool runs the ``samtools stats`` command. It has the following options::
+
+    -c, --coverage <int>,<int>,<int>    Coverage distribution min,max,step [1,1000,1]
+    -d, --remove-dups                   Exclude from statistics reads marked as duplicates
+    -f, --required-flag  <str|int>      Required flag, 0 for unset. See also `samtools flags` [0]
+    -F, --filtering-flag <str|int>      Filtering flag, 0 for unset. See also `samtools flags` [0]
+        --GC-depth <float>              the size of GC-depth bins (decreasing bin size increases memory requirement) [2e4]
+    -h, --help                          This help message
+    -i, --insert-size <int>             Maximum insert size [8000]
+    -I, --id <string>                   Include only listed read group or sample name
+    -l, --read-length <int>             Include in the statistics only reads with the given read length []
+    -m, --most-inserts <float>          Report only the main part of inserts [0.99]
+    -q, --trim-quality <int>            The BWA trimming parameter [0]
+    -r, --ref-seq <file>                Reference sequence (required for GC-depth and mismatches-per-cycle calculation). Galaxy
+                                        will provide options for selecting a reference cached as this Galaxy instance or choosing
+                                        one from history.
+   
+
+    ]]></help>
+    <expand macro="citations"></expand>
+</tool>
+
diff --git a/tool_collections/samtools/samtools_stats/samtools_stats_tool_test_output.html b/tool_collections/samtools/samtools_stats/samtools_stats_tool_test_output.html
new file mode 100644
index 00000000000..fbba3d7b786
--- /dev/null
+++ b/tool_collections/samtools/samtools_stats/samtools_stats_tool_test_output.html
@@ -0,0 +1,372 @@
+<!DOCTYPE html>
+<html lang="en">
+  <head>
+    <meta charset="utf-8">
+    <meta http-equiv="X-UA-Compatible" content="IE=edge">
+    <meta name="viewport" content="width=device-width, initial-scale=1">
+    <title>Tool Test Results (powered by Planemo)</title>
+
+    <!-- Bootstrap -->
+    <style>/*!
+ * Bootstrap v3.3.1 (http://getbootstrap.com)
+ * Copyright 2011-2014 Twitter, Inc.
+ * Licensed under MIT (https://github.com/twbs/bootstrap/blob/master/LICENSE)
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+    <style>/* bootstrap custom style stuff - taken from demo template. */
+/*
+ * Base structure
+ */
+
+/* Move down content because we have a fixed navbar that is 50px tall */
+body {
+  padding-top: 50px;
+}
+
+
+/*
+ * Global add-ons
+ */
+
+.sub-header {
+  padding-bottom: 10px;
+  border-bottom: 1px solid #eee;
+}
+
+/*
+ * Top navigation
+ * Hide default border to remove 1px line.
+ */
+.navbar-fixed-top {
+  border: 0;
+}
+
+/*
+ * Sidebar
+ */
+
+/* Hide for mobile, show later */
+.sidebar {
+  display: none;
+}
+@media (min-width: 768px) {
+  .sidebar {
+    position: fixed;
+    top: 51px;
+    bottom: 0;
+    left: 0;
+    z-index: 1000;
+    display: block;
+    padding: 20px;
+    overflow-x: hidden;
+    overflow-y: auto; /* Scrollable contents if viewport is shorter than content. */
+    background-color: #f5f5f5;
+    border-right: 1px solid #eee;
+  }
+}
+
+/* Sidebar navigation */
+.nav-sidebar {
+  margin-right: -21px; /* 20px padding + 1px border */
+  margin-bottom: 20px;
+  margin-left: -20px;
+}
+.nav-sidebar > li > a {
+  padding-right: 20px;
+  padding-left: 20px;
+}
+.nav-sidebar > .active > a,
+.nav-sidebar > .active > a:hover,
+.nav-sidebar > .active > a:focus {
+  color: #fff;
+  background-color: #428bca;
+}
+
+
+/*
+ * Main content
+ */
+
+.main {
+  padding: 20px;
+}
+@media (min-width: 768px) {
+  .main {
+    padding-right: 40px;
+    padding-left: 40px;
+  }
+}
+.main .page-header {
+  margin-top: 0;
+}
+
+
+/*
+ * Placeholder dashboard ideas
+ */
+
+.placeholders {
+  margin-bottom: 30px;
+  text-align: center;
+}
+.placeholders h4 {
+  margin-bottom: 0;
+}
+.placeholder {
+  margin-bottom: 20px;
+}
+.placeholder img {
+  display: inline-block;
+  border-radius: 50%;
+}
+
+.text-success-custom{
+    color: rgba(6, 108, 8, 1);
+}
+.text-danger-custom{
+    font-weight: bold;
+    color: rgba(213, 24, 20, 1);
+}
+.panel-danger-custom .panel-title{
+    font-weight: bold;
+    color: rgba(213, 24, 20, 1);
+}t status
+
+.panel-success-custom .panel-title{
+    color: rgba(6, 108, 8, 1);
+}t status
+</style>
+
+    <!-- HTML5 shim and Respond.js for IE8 support of HTML5 elements and media queries -->
+    <!-- WARNING: Respond.js doesn't work if you view the page via file:// -->
+    <!--[if lt IE 9]>
+      <script src="https://oss.maxcdn.com/html5shiv/3.7.2/html5shiv.min.js"></script>
+      <script src="https://oss.maxcdn.com/respond/1.4.2/respond.min.js"></script>
+    <![endif]-->
+
+  </head>
+  <body>
+
+    <nav class="navbar navbar-inverse navbar-fixed-top" role="navigation">
+      <div class="container-fluid">
+        <div class="navbar-header">
+
+          <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target="#navbar" aria-expanded="false" aria-controls="navbar">
+            <span class="sr-only">Toggle navigation</span>
+            <span class="icon-bar"></span>
+            <span class="icon-bar"></span>
+            <span class="icon-bar"></span>
+          </button>
+          <a class="navbar-brand" href="#">Tool Test Results (powered by Planemo)</a>
+        </div>
+        <div id="navbar" class="navbar-collapse collapse">
+          <ul class="nav navbar-nav navbar-right">
+            
+    <li><a href="https://galaxyproject.org">Galaxy</a></li>
+    <li><a href="https://planemo.readthedocs.org">Planemo</a></li>
+
+          </ul>
+          <div class="navbar-form navbar-right">
+          </div>
+        </div>
+      </div>
+    </nav>
+
+    <div class="container-fluid">
+      <div class="row">
+        <div class="col-sm-3 col-md-2 sidebar">
+          <ul class="nav nav-sidebar">
+            <li><a href="#overview" class="text-success"><strong>Overview</strong></a></li>
+          </ul>
+          <ul class="nav nav-sidebar" id="nav-sidebar-tests">
+          </ul>
+        </div>
+        <div class="col-sm-9 col-sm-offset-3 col-md-10 col-md-offset-2 main">
+          <!-- <h1 class="page-header">Tests</h1> -->
+          <h2 id="overview">Overview</h2>
+          <div id="overview-content"></div>
+          <div class="progress">
+          </div>
+          <h2 id="tests">Tests</h2>
+          <p>The remainder of this contains a description for each test executed to run these jobs.</p>
+        </div>
+      </div>
+    </div>
+
+    <script>/*! jQuery v2.1.1 | (c) 2005, 2014 jQuery Foundation, Inc. | jquery.org/license */
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+    <script>
+var renderTestResults = function(testData) {
+	var summary = testData["summary"];
+	var numTests = summary["num_tests"];
+	var numProblems = summary["num_errors"] + summary["num_failures"] + summary["num_skips"];
+	var $overview = $("#overview-content");
+	var $progress = $(".progress");
+	if(numTests == 0) {
+		$overview.addClass("alert").addClass("alert-danger").text("No tests were executed.");
+		$progress.append($('<div class="progress-bar progress-bar-warning" role="progressbar" style="width: 100%" />'));
+	} else if(numProblems > 0) {
+		$overview.addClass("alert").addClass("alert-danger").text("There were problems with " + numProblems + " test(s) out of " + numTests + ".");
+		var problemPercent = (numProblems/(1.0 * numTests)) * 100.0;
+		var successPercent = 100.0 - problemPercent;
+		$progress.append($('<div class="progress-bar progress-bar-success" role="progressbar" style="width: ' + successPercent  +  '%" />'));
+		$progress.append($('<div class="progress-bar progress-bar-danger" role="progressbar" style="width: ' + problemPercent  +  '%" />'));
+	} else {
+		$overview.addClass("alert").addClass("alert-success").text("All " + numTests + " test(s) successfully executed.");
+		$progress.append($('<div class="progress-bar progress-bar-success" role="progressbar" style="width: 100%" />'));
+	}
+
+	var $sidebar = $("#nav-sidebar-tests");
+	for(var index in testData["tests"]) {
+		var test = testData["tests"][index];
+		var testResult = new TestResult(test);
+		var rawId = testResult.rawId;
+
+		var panelType = testResult.passed ? "panel-success panel-success-custom" : "panel-danger panel-danger-custom";
+		var $panel = $('<div class="panel">');
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+
+		var $panelHeading = $('<div class="panel-heading">');
+		var $panelTitle = $('<div class="panel-title">');
+		var $a = $('<a class="collapsed" data-toggle="collapse">');
+		$a.attr("id", rawId);
+		$a.attr("data-target", "#collapse"  + index);
+		var testName = testResult.toolName + " (Test #" + (testResult.testIndex + 1) + (testResult.passed ? "" : ", Failed") + ")";
+		$a.text(testName);
+		var $navLink = $('<a>').attr('href', '#' + rawId).text(testName)
+		if(!testResult.passed) {
+			$navLink.addClass("text-danger text-danger-custom");
+		} else {
+			$navLink.addClass("text-success text-success-custom");
+		}
+		$sidebar.append($('<li>').append( $navLink ) );
+		$panelTitle.append($a)
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+		var $panelBody = $('<div class="panel-body panel-collapse collapse" >');
+		$panelBody.attr("id", "collapse" + index);
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+		var $status = $('<div>').text("status: " + testResult.status);
+		$panelBody.append($status);
+		if(testResult.problems.length > 0) {
+			var $problemsLabel = $('<div>').text("problems: ");
+			var $problemsDiv = $('<div style="margin-left:10px;">');
+			var $problemsUl = $('<ul>');
+			for(var problemIndex in testResult.problems) {
+				$problemsUl.append($('<li>').append($('<pre>').text(testResult.problems[problemIndex])));
+			}
+			$problemsDiv.append($problemsUl);
+			$panelBody.append($problemsLabel).append($problemsDiv);
+		}
+		var $commandLabel = $('<div>command:</div>');
+		var $stdoutLabel = $('<div>job standard output:</div>');
+		var $stderrLabel = $('<div>job standard error:</div>');
+		var $command;
+		if(testResult.command !== null) {
+			$command = $('<pre class="pre-scrollable" style="margin-left:10px;">').text(testResult.command);
+		} else {
+			$command = $('<div class="alert alert-warning" style="margin-left:10px;">').text("No command recorded.");
+		}
+		var $stdout;
+		if(testResult.stdout !== null) {
+			$stdout = $('<pre class="pre-scrollable" style="margin-left:10px;">').text(testResult.stdout);
+		} else {
+			$stdout = $('<div class="alert alert-warning" style="margin-left:10px;">').text("No standard output recorded.");
+		}
+		var $stderr;
+		if(testResult.stderr !== null) {
+			$stderr = $('<pre class="pre-scrollable" style="margin-left:10px;">').text(testResult.stderr);
+		} else {
+			$stderr = $('<div class="alert alert-warning" style="margin-left:10px;">').text("No standard error recorded.");
+		}
+		$panelBody
+			.append($commandLabel)
+			.append($command)
+			.append($stdoutLabel)
+			.append($stdout)
+			.append($stderrLabel)
+			.append($stderr);
+		if(!testResult.passed) {
+			var $logLabel = $('<div>log:</div>');
+			var $log = $('<pre class="pre-scrollable" style="margin-left: 10px;">').text(testResult.problemLog);
+			$panelBody.append($logLabel).append($log);
+		}
+
+		$panel.append($panelHeading).append($panelBody);
+		$(".main").append($panel);
+	}
+}
+
+var TestResult = function(data) {
+	this.rawId = data["id"];
+
+	var testMethod = this.rawId.split("TestForTool_")[1];
+	var toolName = testMethod.split(".test_tool_")[0];
+	var testIndex = testMethod.split(".test_tool_")[1];
+	this.toolName = toolName;
+	this.testIndex = parseInt(testIndex);
+	console.log(data);
+	this.status = data["data"]["status"];
+	var job = data["data"]["job"];
+	if(job) {
+		this.stdout = data["data"]["job"]["stdout"];
+		this.stderr = data["data"]["job"]["stderr"];
+		this.command = data["data"]["job"]["command_line"];
+	} else {
+		this.stdout = null;
+		this.stderr = null;
+		this.command = null;
+	}
+	this.problems = [];
+	var outputProblems = data["data"]["output_problems"] || [];
+	var executionProblem = data["data"]["execution_problem"];
+	this.problems.push.apply(this.problems, outputProblems);
+	if(executionProblem) {
+		this.problems.push(executionProblem);
+	}
+	this.problemLog = data["data"]["problem_log"];
+	this.passed = (this.status == "success");
+}
+
+
+// http://stackoverflow.com/questions/19491336/get-url-parameter-jquery
+function getUrlParameter(sParam)
+{
+    var sPageURL = window.location.search.substring(1);
+    var sURLVariables = sPageURL.split('&');
+    for (var i = 0; i < sURLVariables.length; i++)
+    {
+        var sParameterName = sURLVariables[i].split('=');
+        if (sParameterName[0] == sParam)
+        {
+            return sParameterName[1];
+        }
+    }
+}
+</script>
+    <script>
+      var testDataUrl = getUrlParameter("test_data_url");
+      if(testDataUrl) {
+      $.ajax(
+        {'url': testDataUrl,
+         'type': 'GET',
+        }
+        )
+        .success(function(content) { renderTestResults( $.parseJSON(content) ); })
+        .failure(function() { alert("Failed to load test data.")} );
+      } else {
+        var test_data = {"tests": [{"data": {"status": "failure", "inputs": {"use_reference|reference_source|ref_file": {"src": "hda", "id": "5729865256bc2525"}, "use_reference|reference_source|reference_source_selector": "history", "use_reference|use_ref_selector": "yes", "input_file": {"src": "hda", "id": "2891970512fa2d5a"}}, "output_problems": ["History item  different than expected, difference (using diff):\n( /Users/marten/devel/git/tools-devteam/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out1.tab v. /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpVPmlQNsamtools_stats_out1.tab )\n--- local_file\n+++ history_data\n@@ -1,5 +1,5 @@\n-# This file was produced by samtools stats (1.2+htslib-1.2.1) and can be plotted using plot-bamstats\n-# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /Users/anton/galaxy-git/database/files/000/dataset_9.dat /Users/anton/galaxy-git/database/files/000/dataset_10.dat\n+# This file was produced by samtools stats (1.2+htslib-1.2) and can be plotted using plot-bamstats\n+# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_2.dat /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_1.dat\n # CHK, Checksum\t[2]Read Names\t[3]Sequences\t[4]Qualities\n # CHK, CRC32 of reads which passed filtering followed by addition (32bit overflow)\n CHK\t1bd20fd8\t58ad2167\t29883386\n"], "job": {"inputs": {"ref_file": {"src": "hda", "id": "5729865256bc2525"}, "input_file": {"src": "hda", "id": "2891970512fa2d5a"}}, "update_time": "2015-04-21T21:45:40.695226", "tool_id": "samtools_stats", "outputs": {"output": {"src": "hda", "id": "54f2a3a23292eb07"}}, "stdout": "", "command_line": "ln -s \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_2.dat\" && samtools faidx `basename \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_2.dat\"` && samtools stats \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_1.dat\" --coverage 1,1000,1  --GC-depth 20000.0 --insert-size 8000    --most-inserts 0.99 --trim-quality 0 --ref-seq \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_2.dat\" > \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_3.dat\"", "exit_code": 0, "state": "ok", "create_time": "2015-04-21T21:45:29.298433", "params": {"coverage_max": "\"1000\"", "gc_depth": "\"20000.0\"", "insert_size": "\"8000\"", "most_inserts": "\"0.99\"", "coverage_step": "\"1\"", "dbkey": "\"hg17\"", "coverage_min": "\"1\"", "read_length": "\"0\"", "trim_quality": "\"0\"", "filter_by_flags": "{\"filter_flags\": \"nofilter\", \"__current_case__\": 1}", "split_output": "{\"split_output_selector\": \"no\", \"__current_case__\": 0}", "use_reference": "{\"reference_source\": {\"ref_file\": 2, \"reference_source_selector\": \"history\", \"__current_case__\": 1}, \"use_ref_selector\": \"yes\", \"__current_case__\": 0}", "chromInfo": "\"/Users/marten/devel/git/galaxy/tool-data/shared/ucsc/chrom/hg17.len\"", "remove_dups": "\"False\""}, "stderr": "[fai_load] build FASTA index.\n", "job_metrics": [], "model_class": "Job", "external_id": "64545", "id": "54f2a3a23292eb07", "user_email": "test@bx.psu.edu"}, "problem_log": "Traceback (most recent call last):\n  File \"/System/Library/Frameworks/Python.framework/Versions/2.7/lib/python2.7/unittest/case.py\", line 331, in run\n    testMethod()\n  File \"/Users/marten/devel/git/galaxy/test/functional/test_toolbox.py\", line 268, in test_tool\n    self.do_it( td )\n  File \"/Users/marten/devel/git/galaxy/test/functional/test_toolbox.py\", line 67, in do_it\n    raise e\nJobOutputsError: History item  different than expected, difference (using diff):\n( /Users/marten/devel/git/tools-devteam/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out1.tab v. /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpVPmlQNsamtools_stats_out1.tab )\n--- local_file\n+++ history_data\n@@ -1,5 +1,5 @@\n-# This file was produced by samtools stats (1.2+htslib-1.2.1) and can be plotted using plot-bamstats\n-# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /Users/anton/galaxy-git/database/files/000/dataset_9.dat /Users/anton/galaxy-git/database/files/000/dataset_10.dat\n+# This file was produced by samtools stats (1.2+htslib-1.2) and can be plotted using plot-bamstats\n+# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_2.dat /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_1.dat\n # CHK, Checksum\t[2]Read Names\t[3]Sequences\t[4]Qualities\n # CHK, CRC32 of reads which passed filtering followed by addition (32bit overflow)\n CHK\t1bd20fd8\t58ad2167\t29883386\n\n-------------------- >> begin captured logging << --------------------\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.web.framework.webapp: INFO: Session authenticated using Galaxy master api key\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/users?key=test_key HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.web.framework.webapp: INFO: Session authenticated using Galaxy master api key\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/users HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.web.framework.webapp: INFO: Session authenticated using Galaxy master api key\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/users/2891970512fa2d5a/api_key HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/histories HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.tools.actions.upload_common: INFO: tool upload1 created job id 1\ngalaxy.tools.execute: DEBUG: Tool [upload1] created job [1] (721.369 ms)\ngalaxy.jobs: DEBUG: (1) Working directory for job is: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1\ngalaxy.jobs.handler: DEBUG: (1) Dispatching to local runner\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/tools HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs: DEBUG: (1) Persisting job destination (destination id: local:///)\ngalaxy.jobs.runners: DEBUG: Job [1] queued (3701.848 ms)\ngalaxy.jobs.handler: INFO: (1) Job dispatched\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs.runners: DEBUG: (1) command is: python /Users/marten/devel/git/galaxy/tools/data_source/upload.py /Users/marten/devel/git/galaxy /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpcOV73W /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpzTLxF3 1:/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1/dataset_1_files:/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_1.dat; return_code=$?; python /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1/set_metadata_aC_Epn.py /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpcOV73W /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1/galaxy.json /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1/metadata_in_HistoryDatasetAssociation_1_2YHWg0,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1/metadata_kwds_HistoryDatasetAssociation_1_xxSWvK,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1/metadata_out_HistoryDatasetAssociation_1_gpHI_B,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1/metadata_results_HistoryDatasetAssociation_1_bOBqlz,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_1.dat,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1/metadata_override_HistoryDatasetAssociation_1_chT4qY; sh -c \"exit $return_code\"\ngalaxy.jobs.runners.local: DEBUG: (1) executing job script: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1/galaxy_1.sh\ngalaxy.jobs: DEBUG: (1) Persisting job destination (destination id: local:///)\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\ngalaxy.jobs.runners.local: DEBUG: execution finished: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1/galaxy_1.sh\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs: DEBUG: job 1 ended (finish() executed in (3319.682 ms))\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.tools.actions.upload_common: INFO: tool upload1 created job id 2\ngalaxy.tools.execute: DEBUG: Tool [upload1] created job [2] (1436.682 ms)\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/tools HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs: DEBUG: (2) Working directory for job is: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2\ngalaxy.jobs.handler: DEBUG: (2) Dispatching to local runner\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs: DEBUG: (2) Persisting job destination (destination id: local:///)\ngalaxy.jobs.runners: DEBUG: Job [2] queued (2933.918 ms)\ngalaxy.jobs.handler: INFO: (2) Job dispatched\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs.runners: DEBUG: (2) command is: python /Users/marten/devel/git/galaxy/tools/data_source/upload.py /Users/marten/devel/git/galaxy /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpcOV73W /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpQj7Vtj 2:/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2/dataset_2_files:/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_2.dat; return_code=$?; python /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2/set_metadata_EnSKJC.py /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpcOV73W /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2/galaxy.json /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2/metadata_in_HistoryDatasetAssociation_2_EbkTIH,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2/metadata_kwds_HistoryDatasetAssociation_2_A_ZJxl,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2/metadata_out_HistoryDatasetAssociation_2_rYAxQD,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2/metadata_results_HistoryDatasetAssociation_2_1V3qgk,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_2.dat,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2/metadata_override_HistoryDatasetAssociation_2_LI0aVu; sh -c \"exit $return_code\"\ngalaxy.jobs.runners.local: DEBUG: (2) executing job script: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2/galaxy_2.sh\ngalaxy.jobs: DEBUG: (2) Persisting job destination (destination id: local:///)\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs.runners.local: DEBUG: execution finished: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2/galaxy_2.sh\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs: DEBUG: job 2 ended (finish() executed in (5509.688 ms))\ngalaxy.tools.actions: INFO: Handled output (77.015 ms)\ngalaxy.tools.actions: INFO: Verified access to datasets (55.049 ms)\ngalaxy.tools.execute: DEBUG: Tool [samtools_stats] created job [3] (665.725 ms)\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/tools HTTP/1.1\" 200 None\ngalaxy.jobs: DEBUG: (3) Working directory for job is: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/3\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs.handler: DEBUG: (3) Dispatching to local runner\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/54f2a3a23292eb07?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\ngalaxy.jobs: DEBUG: (3) Persisting job destination (destination id: local:///)\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs.runners: DEBUG: Job [3] queued (1882.584 ms)\ngalaxy.jobs.handler: INFO: (3) Job dispatched\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/54f2a3a23292eb07?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/54f2a3a23292eb07?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\ngalaxy.jobs.runners: DEBUG: (3) command is: samtools --version | head -n 1 | awk '{ print $2 }' > /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/GALAXY_VERSION_STRING_3 2>&1; ln -s \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_2.dat\" && samtools faidx `basename \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_2.dat\"` && samtools stats \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_1.dat\" --coverage 1,1000,1  --GC-depth 20000.0 --insert-size 8000    --most-inserts 0.99 --trim-quality 0 --ref-seq \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_2.dat\" > \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_3.dat\"; return_code=$?; python /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/3/set_metadata_Zey9XK.py /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpcOV73W /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/3/galaxy.json /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/3/metadata_in_HistoryDatasetAssociation_3_c241v6,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/3/metadata_kwds_HistoryDatasetAssociation_3_TUAqpX,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/3/metadata_out_HistoryDatasetAssociation_3_fh3HTD,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/3/metadata_results_HistoryDatasetAssociation_3_y4t5DA,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_3.dat,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/3/metadata_override_HistoryDatasetAssociation_3_3rhcZs; sh -c \"exit $return_code\"\ngalaxy.jobs.runners.local: DEBUG: (3) executing job script: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/3/galaxy_3.sh\ngalaxy.jobs: DEBUG: (3) Persisting job destination (destination id: local:///)\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/54f2a3a23292eb07?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\ngalaxy.jobs.runners.local: DEBUG: execution finished: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/3/galaxy_3.sh\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/54f2a3a23292eb07?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\ngalaxy.jobs: DEBUG: job 3 ended (finish() executed in (2080.708 ms))\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/54f2a3a23292eb07?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/54f2a3a23292eb07?full=true&key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/54f2a3a23292eb07?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a/contents/54f2a3a23292eb07/display?raw=true&key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nbase.twilltestcase: DEBUG: keepoutdir: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/jobfiles, ofn: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/jobfiles/samtools_stats_out1.tab\nbase.twilltestcase: DEBUG: ## GALAXY_TEST_SAVE=/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/jobfiles. saved /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/jobfiles/samtools_stats_out1.tab\nbase.twilltestcase: INFO: ## files diff on /Users/marten/devel/git/tools-devteam/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out1.tab and /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpVPmlQNsamtools_stats_out1.tab lines_diff=2, found diff = 4\n--------------------- >> end captured logging << ---------------------\n", "problem_type": "functional.test_toolbox.JobOutputsError"}, "id": "functional.test_toolbox.TestForTool_samtools_stats.test_tool_000000", "has_data": true}, {"data": {"status": "failure", "inputs": {"use_reference|reference_source|reference_source_selector": "history", "input_file": {"src": "hda", "id": "8155e4b4bf1581ff"}, "split_output|split_output_selector": "yes", "split_output|generate_tables": ["sn", "mpc", "gcc"], "use_reference|use_ref_selector": "yes", "use_reference|reference_source|ref_file": {"src": "hda", "id": "7b55dbb89df8f4e5"}}, "output_problems": ["History item  different than expected, difference (using diff):\n( /Users/marten/devel/git/tools-devteam/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2.tab v. /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpYr1M8Csamtools_stats_out2.tab )\n--- local_file\n+++ history_data\n@@ -1,5 +1,5 @@\n-# This file was produced by samtools stats (1.2+htslib-1.2.1) and can be plotted using plot-bamstats\n-# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /Users/anton/galaxy-git/database/files/000/dataset_9.dat /Users/anton/galaxy-git/database/files/000/dataset_10.dat\n+# This file was produced by samtools stats (1.2+htslib-1.2) and can be plotted using plot-bamstats\n+# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_5.dat /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_4.dat\n # CHK, Checksum\t[2]Read Names\t[3]Sequences\t[4]Qualities\n # CHK, CRC32 of reads which passed filtering followed by addition (32bit overflow)\n CHK\t1bd20fd8\t58ad2167\t29883386\n"], "job": {"inputs": {"ref_file": {"src": "hda", "id": "7b55dbb89df8f4e5"}, "input_file": {"src": "hda", "id": "8155e4b4bf1581ff"}}, "update_time": "2015-04-21T21:46:44.081337", "tool_id": "samtools_stats", "outputs": {"__new_primary_file_output|Mismatches per cycle__": {"src": "hda", "id": "a90a30fafe298e1e"}, "__new_primary_file_output|Summary numbers__": {"src": "hda", "id": "b842d972534ccb3e"}, "__new_primary_file_output|ACGT content per cycle__": {"src": "hda", "id": "683bc220e21425bb"}, "output": {"src": "hda", "id": "fa6d20d0fb68383f"}}, "stdout": "sn,mpc,gcc\n", "command_line": "ln -s \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_5.dat\" && samtools faidx `basename \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_5.dat\"` && samtools stats \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_4.dat\" --coverage 1,1000,1  --GC-depth 20000.0 --insert-size 8000    --most-inserts 0.99 --trim-quality 0 --ref-seq \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_5.dat\" > \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" && mkdir split && echo sn,mpc,gcc &&  echo \"# Summary Numbers\\n\"  > \"split/Summary numbers.tab\" && grep -q ^SN  \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" ; if [ $? = 0 ] ; then grep ^SN  \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" | cut -f 2- >> \"split/Summary numbers.tab\"  ; fi &&    echo \"# Columns correspond to qualities, rows to cycles. First column is the cycle number, second is the number of N's and the rest is the number of mismatches\" > \"split/Mismatches per cycle.tab\" && grep -q ^MPC \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" ; if [ $? = 0 ] ; then grep ^MPC \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" | cut -f 2- >> \"split/Mismatches per cycle.tab\" ; fi &&    echo \"# ACGT content per cycle. The columns are: cycle, and A,C,G,T counts (percent)\" > \"split/ACGT content per cycle.tab\" && grep -q ^GCC \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" ; if [ $? = 0 ] ; then grep ^GCC \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" | cut -f 2- >> \"split/ACGT content per cycle.tab\" ; fi &&         true", "exit_code": 0, "state": "ok", "create_time": "2015-04-21T21:46:23.415078", "params": {"coverage_max": "\"1000\"", "gc_depth": "\"20000.0\"", "insert_size": "\"8000\"", "most_inserts": "\"0.99\"", "coverage_step": "\"1\"", "dbkey": "\"hg17\"", "coverage_min": "\"1\"", "read_length": "\"0\"", "trim_quality": "\"0\"", "filter_by_flags": "{\"filter_flags\": \"nofilter\", \"__current_case__\": 1}", "split_output": "{\"generate_tables\": [\"sn\", \"mpc\", \"gcc\"], \"split_output_selector\": \"yes\", \"__current_case__\": 1}", "use_reference": "{\"reference_source\": {\"ref_file\": 5, \"reference_source_selector\": \"history\", \"__current_case__\": 1}, \"use_ref_selector\": \"yes\", \"__current_case__\": 0}", "chromInfo": "\"/Users/marten/devel/git/galaxy/tool-data/shared/ucsc/chrom/hg17.len\"", "remove_dups": "\"False\""}, "stderr": "[fai_load] build FASTA index.\n", "job_metrics": [], "model_class": "Job", "external_id": "64605", "id": "fa6d20d0fb68383f", "user_email": "test@bx.psu.edu"}, "problem_log": "Traceback (most recent call last):\n  File \"/System/Library/Frameworks/Python.framework/Versions/2.7/lib/python2.7/unittest/case.py\", line 331, in run\n    testMethod()\n  File \"/Users/marten/devel/git/galaxy/test/functional/test_toolbox.py\", line 268, in test_tool\n    self.do_it( td )\n  File \"/Users/marten/devel/git/galaxy/test/functional/test_toolbox.py\", line 67, in do_it\n    raise e\nJobOutputsError: History item  different than expected, difference (using diff):\n( /Users/marten/devel/git/tools-devteam/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2.tab v. /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpYr1M8Csamtools_stats_out2.tab )\n--- local_file\n+++ history_data\n@@ -1,5 +1,5 @@\n-# This file was produced by samtools stats (1.2+htslib-1.2.1) and can be plotted using plot-bamstats\n-# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /Users/anton/galaxy-git/database/files/000/dataset_9.dat /Users/anton/galaxy-git/database/files/000/dataset_10.dat\n+# This file was produced by samtools stats (1.2+htslib-1.2) and can be plotted using plot-bamstats\n+# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_5.dat /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_4.dat\n # CHK, Checksum\t[2]Read Names\t[3]Sequences\t[4]Qualities\n # CHK, CRC32 of reads which passed filtering followed by addition (32bit overflow)\n CHK\t1bd20fd8\t58ad2167\t29883386\n\n-------------------- >> begin captured logging << --------------------\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.web.framework.webapp: INFO: Session authenticated using Galaxy master api key\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/users?key=test_key HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.web.framework.webapp: INFO: Session authenticated using Galaxy master api key\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/users/2891970512fa2d5a/api_key HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/histories HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.tools.actions.upload_common: INFO: tool upload1 created job id 4\ngalaxy.tools.execute: DEBUG: Tool [upload1] created job [4] (488.979 ms)\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/tools HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs: DEBUG: (4) Working directory for job is: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs.handler: DEBUG: (4) Dispatching to local runner\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs: DEBUG: (4) Persisting job destination (destination id: local:///)\ngalaxy.jobs.runners: DEBUG: Job [4] queued (3770.035 ms)\ngalaxy.jobs.handler: INFO: (4) Job dispatched\ngalaxy.jobs.runners: DEBUG: (4) command is: python /Users/marten/devel/git/galaxy/tools/data_source/upload.py /Users/marten/devel/git/galaxy /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpcOV73W /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpovV4Rg 4:/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4/dataset_4_files:/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_4.dat; return_code=$?; python /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4/set_metadata_pDUWU1.py /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpcOV73W /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4/galaxy.json /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4/metadata_in_HistoryDatasetAssociation_4_KJFVUK,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4/metadata_kwds_HistoryDatasetAssociation_4_eC_v98,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4/metadata_out_HistoryDatasetAssociation_4_PVfJHp,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4/metadata_results_HistoryDatasetAssociation_4_JUuyR2,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_4.dat,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4/metadata_override_HistoryDatasetAssociation_4_OMLQxN; sh -c \"exit $return_code\"\ngalaxy.jobs.runners.local: DEBUG: (4) executing job script: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4/galaxy_4.sh\ngalaxy.jobs: DEBUG: (4) Persisting job destination (destination id: local:///)\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\ngalaxy.jobs.runners.local: DEBUG: execution finished: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4/galaxy_4.sh\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs: DEBUG: job 4 ended (finish() executed in (7314.557 ms))\ngalaxy.tools.actions.upload_common: INFO: tool upload1 created job id 5\ngalaxy.tools.execute: DEBUG: Tool [upload1] created job [5] (380.201 ms)\ngalaxy.jobs: DEBUG: (5) Working directory for job is: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/tools HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs.handler: DEBUG: (5) Dispatching to local runner\ngalaxy.jobs: DEBUG: (5) Persisting job destination (destination id: local:///)\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs.runners: DEBUG: Job [5] queued (2761.054 ms)\ngalaxy.jobs.handler: INFO: (5) Job dispatched\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs.runners: DEBUG: (5) command is: python /Users/marten/devel/git/galaxy/tools/data_source/upload.py /Users/marten/devel/git/galaxy /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpcOV73W /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpx_FyDt 5:/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5/dataset_5_files:/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_5.dat; return_code=$?; python /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5/set_metadata_ErwZWr.py /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpcOV73W /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5/galaxy.json /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5/metadata_in_HistoryDatasetAssociation_5_bf6GWj,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5/metadata_kwds_HistoryDatasetAssociation_5_qdTYxt,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5/metadata_out_HistoryDatasetAssociation_5_nILjxU,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5/metadata_results_HistoryDatasetAssociation_5_nF9gHk,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_5.dat,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5/metadata_override_HistoryDatasetAssociation_5_xELsea; sh -c \"exit $return_code\"\ngalaxy.jobs.runners.local: DEBUG: (5) executing job script: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5/galaxy_5.sh\ngalaxy.jobs: DEBUG: (5) Persisting job destination (destination id: local:///)\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\ngalaxy.jobs.runners.local: DEBUG: execution finished: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5/galaxy_5.sh\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.tools.actions: INFO: Handled output (619.072 ms)\nsqlalchemy.pool.NullPool: ERROR: Exception during reset or similar\nTraceback (most recent call last):\n  File \"build/bdist.macosx-10.6-intel/egg/sqlalchemy/pool.py\", line 567, in _finalize_fairy\n    fairy._reset(pool)\n  File \"build/bdist.macosx-10.6-intel/egg/sqlalchemy/pool.py\", line 701, in _reset\n    pool._dialect.do_rollback(self)\n  File \"build/bdist.macosx-10.6-intel/egg/sqlalchemy/engine/default.py\", line 412, in do_rollback\n    dbapi_connection.rollback()\nProgrammingError: SQLite objects created in a thread can only be used in that same thread.The object was created in thread id 4568608768 and this is thread id 4471201792\nsqlalchemy.pool.NullPool: ERROR: Exception closing connection <pysqlite2.dbapi2.Connection object at 0x111207ce0>\nTraceback (most recent call last):\n  File \"build/bdist.macosx-10.6-intel/egg/sqlalchemy/pool.py\", line 251, in _close_connection\n    self._dialect.do_close(connection)\n  File \"build/bdist.macosx-10.6-intel/egg/sqlalchemy/engine/default.py\", line 418, in do_close\n    dbapi_connection.close()\nProgrammingError: SQLite objects created in a thread can only be used in that same thread.The object was created in thread id 4568608768 and this is thread id 4471201792\ngalaxy.jobs: DEBUG: job 5 ended (finish() executed in (6572.427 ms))\ngalaxy.tools.actions: INFO: Verified access to datasets (340.159 ms)\ngalaxy.tools.execute: DEBUG: Tool [samtools_stats] created job [6] (4823.769 ms)\ngalaxy.jobs: DEBUG: (6) Working directory for job is: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/6\ngalaxy.jobs.handler: DEBUG: (6) Dispatching to local runner\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/tools HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs: DEBUG: (6) Persisting job destination (destination id: local:///)\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/fa6d20d0fb68383f?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\ngalaxy.jobs.runners: DEBUG: Job [6] queued (3581.802 ms)\ngalaxy.jobs.handler: INFO: (6) Job dispatched\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/fa6d20d0fb68383f?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/fa6d20d0fb68383f?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/fa6d20d0fb68383f?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\ngalaxy.jobs.runners: DEBUG: (6) command is: samtools --version | head -n 1 | awk '{ print $2 }' > /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/GALAXY_VERSION_STRING_6 2>&1; ln -s \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_5.dat\" && samtools faidx `basename \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_5.dat\"` && samtools stats \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_4.dat\" --coverage 1,1000,1  --GC-depth 20000.0 --insert-size 8000    --most-inserts 0.99 --trim-quality 0 --ref-seq \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_5.dat\" > \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" && mkdir split && echo sn,mpc,gcc &&  echo \"# Summary Numbers\\n\"  > \"split/Summary numbers.tab\" && grep -q ^SN  \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" ; if [ $? = 0 ] ; then grep ^SN  \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" | cut -f 2- >> \"split/Summary numbers.tab\"  ; fi &&    echo \"# Columns correspond to qualities, rows to cycles. First column is the cycle number, second is the number of N's and the rest is the number of mismatches\" > \"split/Mismatches per cycle.tab\" && grep -q ^MPC \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" ; if [ $? = 0 ] ; then grep ^MPC \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" | cut -f 2- >> \"split/Mismatches per cycle.tab\" ; fi &&    echo \"# ACGT content per cycle. The columns are: cycle, and A,C,G,T counts (percent)\" > \"split/ACGT content per cycle.tab\" && grep -q ^GCC \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" ; if [ $? = 0 ] ; then grep ^GCC \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" | cut -f 2- >> \"split/ACGT content per cycle.tab\" ; fi &&         true; return_code=$?; python /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/6/set_metadata_LSTtun.py /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpcOV73W /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/6/galaxy.json /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/6/metadata_in_HistoryDatasetAssociation_6_uh9zmy,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/6/metadata_kwds_HistoryDatasetAssociation_6_6GLt0N,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/6/metadata_out_HistoryDatasetAssociation_6_OK7DHx,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/6/metadata_results_HistoryDatasetAssociation_6_GeXlkW,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/6/metadata_override_HistoryDatasetAssociation_6_ZyuaJN; sh -c \"exit $return_code\"\ngalaxy.jobs.runners.local: DEBUG: (6) executing job script: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/6/galaxy_6.sh\ngalaxy.jobs: DEBUG: (6) Persisting job destination (destination id: local:///)\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/fa6d20d0fb68383f?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\ngalaxy.jobs.runners.local: DEBUG: execution finished: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/6/galaxy_6.sh\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/fa6d20d0fb68383f?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\ngalaxy.jobs: DEBUG: job 6 ended (finish() executed in (6680.679 ms))\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/fa6d20d0fb68383f?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/fa6d20d0fb68383f?full=true&key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/fa6d20d0fb68383f?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525/contents/fa6d20d0fb68383f/display?raw=true&key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nbase.twilltestcase: DEBUG: keepoutdir: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/jobfiles, ofn: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/jobfiles/samtools_stats_out2.tab\nbase.twilltestcase: DEBUG: ## GALAXY_TEST_SAVE=/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/jobfiles. saved /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/jobfiles/samtools_stats_out2.tab\nbase.twilltestcase: INFO: ## files diff on /Users/marten/devel/git/tools-devteam/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2.tab and /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpYr1M8Csamtools_stats_out2.tab lines_diff=2, found diff = 4\n--------------------- >> end captured logging << ---------------------\n", "problem_type": "functional.test_toolbox.JobOutputsError"}, "id": "functional.test_toolbox.TestForTool_samtools_stats.test_tool_000001", "has_data": true}], "version": "0.1", "summary": {"num_skips": 0, "num_errors": 0, "num_failures": 2, "num_tests": 2}};
+        renderTestResults(test_data);
+      }
+    </script>
+  </body>
+</html>
diff --git a/tool_collections/samtools/samtools_stats/test-data/phiX.bam b/tool_collections/samtools/samtools_stats/test-data/phiX.bam
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diff --git a/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out1.tab b/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out1.tab
new file mode 100644
index 00000000000..7867a751100
--- /dev/null
+++ b/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out1.tab
@@ -0,0 +1,1137 @@
+# This file was produced by samtools stats (1.2+htslib-1.2.1) and can be plotted using plot-bamstats
+# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /Users/anton/galaxy-git/database/files/000/dataset_9.dat /Users/anton/galaxy-git/database/files/000/dataset_10.dat
+# CHK, Checksum	[2]Read Names	[3]Sequences	[4]Qualities
+# CHK, CRC32 of reads which passed filtering followed by addition (32bit overflow)
+CHK	1bd20fd8	58ad2167	29883386
+# Summary Numbers. Use `grep ^SN | cut -f 2-` to extract this part.
+SN	raw total sequences:	200
+SN	filtered sequences:	0
+SN	sequences:	200
+SN	is sorted:	1
+SN	1st fragments:	100
+SN	last fragments:	100
+SN	reads mapped:	25
+SN	reads mapped and paired:	0	# paired-end technology bit set + both mates mapped
+SN	reads unmapped:	175
+SN	reads properly paired:	0	# proper-pair bit set
+SN	reads paired:	200	# paired-end technology bit set
+SN	reads duplicated:	0	# PCR or optical duplicate bit set
+SN	reads MQ0:	6	# mapped and MQ=0
+SN	reads QC failed:	0
+SN	non-primary alignments:	0
+SN	total length:	50200	# ignores clipping
+SN	bases mapped:	6275	# ignores clipping
+SN	bases mapped (cigar):	6275	# more accurate
+SN	bases trimmed:	0
+SN	bases duplicated:	0
+SN	mismatches:	591	# from NM fields
+SN	error rate:	9.418327e-02	# mismatches / bases mapped (cigar)
+SN	average length:	251
+SN	maximum length:	251
+SN	average quality:	34.7
+SN	insert size average:	0.0
+SN	insert size standard deviation:	0.0
+SN	inward oriented pairs:	0
+SN	outward oriented pairs:	0
+SN	pairs with other orientation:	0
+SN	pairs on different chromosomes:	0
+# First Fragment Qualitites. Use `grep ^FFQ | cut -f 2-` to extract this part.
+# Columns correspond to qualities and rows to cycles. First column is the cycle number.
+FFQ	1	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	3	2	0	20	38	32	1	0	0	0	0	0	0
+FFQ	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	3	0	0	18	36	36	6	0	0	0	0	0	0
+FFQ	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	2	1	0	14	31	46	5	0	0	0	0	0	0
+FFQ	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	8	36	42	11	0	0	0	0	0	0
+FFQ	5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	2	0	0	0	0	0	0	0	0	0	0	1	0	0	12	35	43	6	0	0	0	0	0	0
+FFQ	6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	1	7	8	0	0	81	0	0	0	0
+FFQ	7	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	9	13	1	0	76	0	0	0	0
+FFQ	8	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	11	15	4	0	69	0	0	0	0
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+FFQ	187	0	0	0	0	0	0	0	0	0	0	0	0	4	1	3	3	0	0	0	0	0	0	0	0	5	1	1	0	2	0	1	1	6	10	3	2	3	41	10	3	0	0
+FFQ	188	0	0	0	0	0	0	0	0	0	0	0	0	0	5	1	3	0	0	0	0	0	0	0	0	2	4	7	0	1	0	3	2	7	7	6	3	4	34	9	2	0	0
+FFQ	189	0	0	0	0	0	0	0	0	0	0	0	0	3	4	3	4	0	0	0	0	0	0	0	0	4	0	5	2	0	0	5	0	6	8	3	8	8	30	6	1	0	0
+FFQ	190	0	0	0	0	0	0	0	0	0	0	0	0	5	5	3	6	0	0	0	0	0	0	0	0	2	1	3	0	1	0	2	2	7	11	1	8	3	34	6	0	0	0
+FFQ	191	0	0	0	0	0	0	0	0	0	0	0	0	5	7	2	4	0	0	0	0	0	0	0	0	3	0	3	0	0	0	1	0	4	5	9	6	12	33	6	0	0	0
+FFQ	192	0	0	0	0	0	0	0	0	0	0	0	0	3	3	2	6	0	0	0	0	0	0	0	0	3	3	6	0	1	0	0	1	5	9	4	4	5	38	7	0	0	0
+FFQ	193	0	0	0	0	0	0	0	0	0	0	0	0	1	1	2	7	0	0	0	0	0	0	0	0	4	3	3	0	0	0	0	0	5	10	4	2	11	43	4	0	0	0
+FFQ	194	0	0	0	0	0	0	0	0	0	0	0	0	1	1	1	6	0	0	0	0	0	0	0	0	5	1	8	0	1	0	1	0	4	8	2	7	3	43	8	0	0	0
+FFQ	195	0	0	0	0	0	0	0	0	0	0	0	0	4	1	2	8	0	0	0	0	0	0	0	0	0	0	4	0	1	0	3	0	2	13	1	4	8	39	10	0	0	0
+FFQ	196	0	0	0	0	0	0	0	0	0	0	0	0	1	6	1	3	0	0	0	0	0	0	0	0	1	1	3	0	2	0	1	1	4	11	2	1	9	43	10	0	0	0
+FFQ	197	0	0	0	0	0	0	0	0	0	0	0	0	2	0	2	10	0	0	0	0	0	0	0	0	3	0	1	0	0	0	2	1	6	10	2	0	4	47	10	0	0	0
+FFQ	198	0	0	0	0	0	0	0	0	0	0	0	0	2	4	4	7	0	0	0	0	0	0	0	0	1	0	5	1	2	1	0	0	1	7	4	2	4	47	8	0	0	0
+FFQ	199	0	0	0	0	0	0	0	0	0	0	0	0	1	4	5	6	0	0	0	0	0	0	0	0	3	2	7	0	0	0	1	0	3	7	3	3	7	39	9	0	0	0
+FFQ	200	0	0	0	0	0	0	0	0	0	0	0	0	0	5	2	8	0	0	0	0	0	0	0	0	5	1	1	0	1	0	2	0	5	12	5	5	3	40	5	0	0	0
+FFQ	201	0	0	0	0	0	0	0	0	0	0	0	0	2	1	6	7	0	0	0	0	0	0	0	0	5	0	4	0	0	0	1	1	7	8	1	0	4	47	6	0	0	0
+FFQ	202	0	0	0	0	0	0	0	0	0	0	0	0	6	7	3	8	0	0	0	0	0	0	0	0	3	3	1	0	0	0	1	0	4	12	2	3	2	34	11	0	0	0
+FFQ	203	0	0	0	0	0	0	0	0	0	0	0	0	8	5	2	5	0	0	0	0	0	0	0	0	2	0	4	0	1	0	1	1	4	9	7	2	2	37	10	0	0	0
+FFQ	204	0	0	0	0	0	0	0	0	0	0	0	0	2	5	1	6	0	0	0	0	0	0	0	0	4	3	5	0	0	0	0	0	5	11	3	3	5	41	6	0	0	0
+FFQ	205	0	0	0	0	0	0	0	0	0	0	0	0	5	11	1	5	0	0	0	0	0	0	0	0	3	0	3	0	0	0	2	0	4	18	2	4	4	33	5	0	0	0
+FFQ	206	0	0	0	0	0	0	0	0	0	0	0	0	3	7	2	5	0	0	0	0	0	0	0	0	4	5	7	0	0	0	0	0	2	13	0	3	4	37	8	0	0	0
+FFQ	207	0	0	0	0	0	0	0	0	0	0	0	0	1	6	5	6	0	0	0	0	0	0	0	0	4	2	4	0	2	0	4	1	3	10	4	2	3	36	7	0	0	0
+FFQ	208	0	0	0	0	0	0	0	0	0	0	0	0	1	4	3	4	0	0	0	0	0	0	0	0	6	4	4	0	0	0	1	0	9	11	2	5	3	36	7	0	0	0
+FFQ	209	0	0	0	0	0	0	0	0	0	0	0	0	2	7	4	8	0	0	0	0	0	0	0	0	3	2	4	0	0	0	3	0	8	14	2	1	2	33	7	0	0	0
+FFQ	210	0	0	0	0	0	0	0	0	0	0	0	0	1	5	6	7	0	0	0	0	0	0	0	0	4	3	3	0	0	0	3	0	6	6	0	3	5	43	5	0	0	0
+FFQ	211	0	0	0	0	0	0	0	0	0	0	0	0	2	5	6	4	0	0	0	0	0	0	0	0	6	3	5	0	0	0	0	2	5	11	4	3	3	35	6	0	0	0
+FFQ	212	0	0	0	0	0	0	0	0	0	0	0	0	0	3	6	7	0	0	0	0	0	0	0	0	3	4	8	0	0	0	0	0	5	17	1	1	4	35	6	0	0	0
+FFQ	213	0	0	0	0	0	0	0	0	0	0	0	0	6	6	10	5	0	0	0	0	0	0	0	0	5	3	1	0	0	0	0	1	4	7	1	5	2	43	1	0	0	0
+FFQ	214	0	0	0	0	0	0	0	0	0	0	0	0	4	9	4	10	0	0	0	0	0	0	0	0	4	1	6	0	2	0	1	1	3	9	0	2	3	39	2	0	0	0
+FFQ	215	0	0	0	0	0	0	0	0	0	0	0	0	5	6	5	6	0	0	0	0	0	0	0	0	5	5	8	0	0	0	1	2	5	12	0	0	4	34	2	0	0	0
+FFQ	216	0	0	0	0	0	0	0	0	0	0	0	0	3	3	6	5	0	0	0	0	0	0	0	0	3	6	5	1	3	0	1	0	5	15	1	3	2	37	1	0	0	0
+FFQ	217	0	0	0	0	0	0	0	0	0	0	0	0	3	7	6	4	0	0	0	0	0	0	0	0	4	1	2	0	0	1	1	0	10	15	1	3	2	39	1	0	0	0
+FFQ	218	0	0	0	0	0	0	0	0	0	0	0	0	2	4	7	2	0	0	0	0	0	0	0	0	6	4	10	0	0	0	1	0	3	13	1	5	3	35	4	0	0	0
+FFQ	219	0	0	0	0	0	0	0	0	0	0	0	0	2	5	4	4	0	0	0	0	0	0	0	0	5	1	5	0	0	1	0	0	4	13	1	6	3	45	1	0	0	0
+FFQ	220	0	0	0	0	0	0	0	0	0	0	0	0	2	4	9	4	0	0	0	0	0	0	0	0	4	2	2	0	0	0	0	2	5	14	1	1	2	47	1	0	0	0
+FFQ	221	0	0	0	0	0	0	0	0	0	0	0	0	3	4	6	4	0	0	0	0	0	0	0	0	1	4	6	0	0	0	0	0	2	10	1	3	3	53	0	0	0	0
+FFQ	222	0	0	0	0	0	0	0	0	0	0	0	0	3	5	8	6	0	0	0	0	0	0	0	0	5	2	6	0	0	0	0	0	2	6	1	3	4	49	0	0	0	0
+FFQ	223	0	0	0	0	0	0	0	0	0	0	0	0	5	8	6	8	0	0	0	0	0	0	0	0	3	3	5	1	1	0	1	0	0	13	1	0	2	43	0	0	0	0
+FFQ	224	0	0	0	0	0	0	0	0	0	0	0	0	2	9	11	6	0	0	0	0	0	0	0	0	4	3	3	0	0	0	0	1	2	15	1	0	1	42	0	0	0	0
+FFQ	225	0	0	0	0	0	0	0	0	0	0	0	0	4	8	2	4	0	0	0	0	0	0	0	0	7	3	10	0	2	0	0	0	3	10	1	2	5	39	0	0	0	0
+FFQ	226	0	0	0	0	0	0	0	0	0	0	0	0	5	9	3	7	0	0	0	0	0	0	0	0	8	5	5	0	1	0	2	1	3	13	0	2	3	33	0	0	0	0
+FFQ	227	0	0	0	0	0	0	0	0	0	0	0	0	10	8	4	5	0	0	0	0	0	0	0	0	3	2	3	0	0	0	2	3	5	17	0	3	2	33	0	0	0	0
+FFQ	228	0	0	0	0	0	0	0	0	0	0	0	0	10	8	6	2	0	0	0	0	0	0	0	0	6	1	6	0	1	2	1	1	4	13	1	2	1	35	0	0	0	0
+FFQ	229	0	0	0	0	0	0	0	0	0	0	0	0	4	11	6	6	0	0	0	0	0	0	0	0	1	3	5	0	0	0	0	2	6	13	0	3	5	35	0	0	0	0
+FFQ	230	0	0	0	0	0	0	0	0	0	0	0	0	4	11	10	7	0	0	0	0	0	0	0	0	2	3	4	0	0	0	1	2	9	10	0	1	1	35	0	0	0	0
+FFQ	231	0	0	0	0	0	0	0	0	0	0	0	0	6	7	6	6	0	0	0	0	0	0	0	0	4	5	4	0	1	0	1	0	4	13	1	5	5	32	0	0	0	0
+FFQ	232	0	0	0	0	0	0	0	0	0	0	0	0	3	7	10	4	0	0	0	0	0	0	0	0	6	4	7	0	0	0	0	0	3	12	0	1	3	40	0	0	0	0
+FFQ	233	0	0	0	0	0	0	0	0	0	0	0	0	7	7	9	8	0	0	0	0	0	0	0	0	5	5	3	1	1	0	0	0	6	10	1	1	4	32	0	0	0	0
+FFQ	234	0	0	0	0	0	0	0	0	0	0	0	0	9	6	10	5	0	0	0	0	0	0	0	0	5	5	3	1	0	0	0	1	5	11	0	1	4	34	0	0	0	0
+FFQ	235	0	0	0	0	0	0	0	0	0	0	0	0	4	10	13	7	0	0	0	0	0	0	0	0	5	1	4	0	0	0	2	1	5	7	0	0	4	37	0	0	0	0
+FFQ	236	0	0	0	0	0	0	0	0	0	0	0	0	7	8	15	6	0	0	0	0	0	0	0	0	7	3	5	1	0	0	0	1	6	8	0	1	1	31	0	0	0	0
+FFQ	237	0	0	0	0	0	0	0	0	0	0	0	0	2	9	13	6	0	0	0	0	0	0	0	0	6	4	7	1	0	0	0	1	3	12	0	1	1	34	0	0	0	0
+FFQ	238	0	0	0	0	0	0	0	0	0	0	0	0	2	10	16	6	0	0	0	0	0	0	0	0	9	2	3	0	0	0	0	0	5	9	0	2	3	33	0	0	0	0
+FFQ	239	0	0	0	0	0	0	0	0	0	0	0	0	4	5	17	9	0	0	0	0	0	0	0	0	7	3	1	0	0	0	1	1	2	15	0	1	3	31	0	0	0	0
+FFQ	240	0	0	0	0	0	0	0	0	0	0	0	0	0	10	19	6	0	0	0	0	0	0	0	0	6	4	4	0	0	1	3	0	2	13	0	1	1	30	0	0	0	0
+FFQ	241	0	0	0	0	0	0	0	0	0	0	0	0	1	4	23	9	0	0	0	0	0	0	0	0	7	5	8	1	0	0	2	1	1	8	0	1	3	26	0	0	0	0
+FFQ	242	0	0	0	0	0	0	0	0	0	0	0	0	3	10	19	10	0	0	0	0	0	0	0	0	2	5	4	1	0	0	0	1	2	10	0	0	2	31	0	0	0	0
+FFQ	243	0	0	0	0	0	0	0	0	0	0	0	0	2	2	17	14	0	0	0	0	0	0	0	0	6	5	5	0	0	0	0	0	6	13	0	2	3	25	0	0	0	0
+FFQ	244	0	0	0	0	0	0	0	0	0	0	0	0	1	3	18	11	0	0	0	0	0	0	0	0	6	5	5	1	0	0	1	0	2	12	0	5	1	29	0	0	0	0
+FFQ	245	0	0	0	0	0	0	0	0	0	0	0	0	1	8	14	11	0	0	0	0	0	0	0	0	8	5	3	1	0	0	1	0	1	15	1	1	3	27	0	0	0	0
+FFQ	246	0	0	0	0	0	0	0	0	0	0	0	0	4	6	15	12	0	0	0	0	0	0	0	0	5	2	8	1	0	1	1	0	1	13	0	2	1	28	0	0	0	0
+FFQ	247	0	0	0	0	0	0	0	0	0	0	0	0	8	3	16	14	0	0	0	0	0	0	0	0	10	4	6	0	0	0	0	0	2	15	1	2	2	17	0	0	0	0
+FFQ	248	0	0	0	0	0	0	0	0	0	0	0	0	5	6	21	8	0	0	0	0	0	0	0	0	7	8	6	2	0	0	0	1	1	14	0	2	2	17	0	0	0	0
+FFQ	249	0	0	0	0	0	0	0	0	0	0	0	0	5	6	20	8	0	0	0	0	0	0	0	0	5	7	10	0	1	0	0	0	1	15	0	2	0	20	0	0	0	0
+FFQ	250	0	0	0	0	0	0	0	0	0	0	0	0	2	10	15	10	0	0	0	0	0	0	0	0	9	5	8	1	0	0	0	2	6	10	0	0	2	20	0	0	0	0
+FFQ	251	0	0	0	0	0	0	0	0	0	0	0	0	7	20	21	17	0	0	0	0	0	0	0	0	7	7	6	0	0	0	0	1	2	4	0	0	0	8	0	0	0	0
+FFQ	252	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+# Last Fragment Qualitites. Use `grep ^LFQ | cut -f 2-` to extract this part.
+# Columns correspond to qualities and rows to cycles. First column is the cycle number.
+LFQ	1	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	3	2	0	20	38	32	1	0	0	0	0	0	0
+LFQ	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	3	0	0	18	36	36	6	0	0	0	0	0	0
+LFQ	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	2	1	0	14	31	46	5	0	0	0	0	0	0
+LFQ	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	8	36	42	11	0	0	0	0	0	0
+LFQ	5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	2	0	0	0	0	0	0	0	0	0	0	1	0	0	12	35	43	6	0	0	0	0	0	0
+LFQ	6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	1	7	8	0	0	81	0	0	0	0
+LFQ	7	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	9	13	1	0	76	0	0	0	0
+LFQ	8	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	11	15	4	0	69	0	0	0	0
+LFQ	9	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	6	20	3	0	68	0	0	0	0
+LFQ	10	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	7	11	5	0	75	0	0	0	0
+LFQ	11	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	6	16	6	0	70	0	0	0	0
+LFQ	12	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	2	8	2	0	86	0	0	0	0
+LFQ	13	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	3	92	0	0	0
+LFQ	14	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	2	2	1	0	3	89	0	0	0
+LFQ	15	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	2	4	91	0	0	0
+LFQ	16	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	2	96	0	0	0
+LFQ	17	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	3	94	0	0	0
+LFQ	18	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	1	0	2	0	0	2	93	0	0	0
+LFQ	19	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	4	93	0	0	0
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+LFQ	198	0	0	0	0	0	0	0	0	0	0	0	0	2	4	4	7	0	0	0	0	0	0	0	0	1	0	5	1	2	1	0	0	1	7	4	2	4	47	8	0	0	0
+LFQ	199	0	0	0	0	0	0	0	0	0	0	0	0	1	4	5	6	0	0	0	0	0	0	0	0	3	2	7	0	0	0	1	0	3	7	3	3	7	39	9	0	0	0
+LFQ	200	0	0	0	0	0	0	0	0	0	0	0	0	0	5	2	8	0	0	0	0	0	0	0	0	5	1	1	0	1	0	2	0	5	12	5	5	3	40	5	0	0	0
+LFQ	201	0	0	0	0	0	0	0	0	0	0	0	0	2	1	6	7	0	0	0	0	0	0	0	0	5	0	4	0	0	0	1	1	7	8	1	0	4	47	6	0	0	0
+LFQ	202	0	0	0	0	0	0	0	0	0	0	0	0	6	7	3	8	0	0	0	0	0	0	0	0	3	3	1	0	0	0	1	0	4	12	2	3	2	34	11	0	0	0
+LFQ	203	0	0	0	0	0	0	0	0	0	0	0	0	8	5	2	5	0	0	0	0	0	0	0	0	2	0	4	0	1	0	1	1	4	9	7	2	2	37	10	0	0	0
+LFQ	204	0	0	0	0	0	0	0	0	0	0	0	0	2	5	1	6	0	0	0	0	0	0	0	0	4	3	5	0	0	0	0	0	5	11	3	3	5	41	6	0	0	0
+LFQ	205	0	0	0	0	0	0	0	0	0	0	0	0	5	11	1	5	0	0	0	0	0	0	0	0	3	0	3	0	0	0	2	0	4	18	2	4	4	33	5	0	0	0
+LFQ	206	0	0	0	0	0	0	0	0	0	0	0	0	3	7	2	5	0	0	0	0	0	0	0	0	4	5	7	0	0	0	0	0	2	13	0	3	4	37	8	0	0	0
+LFQ	207	0	0	0	0	0	0	0	0	0	0	0	0	1	6	5	6	0	0	0	0	0	0	0	0	4	2	4	0	2	0	4	1	3	10	4	2	3	36	7	0	0	0
+LFQ	208	0	0	0	0	0	0	0	0	0	0	0	0	1	4	3	4	0	0	0	0	0	0	0	0	6	4	4	0	0	0	1	0	9	11	2	5	3	36	7	0	0	0
+LFQ	209	0	0	0	0	0	0	0	0	0	0	0	0	2	7	4	8	0	0	0	0	0	0	0	0	3	2	4	0	0	0	3	0	8	14	2	1	2	33	7	0	0	0
+LFQ	210	0	0	0	0	0	0	0	0	0	0	0	0	1	5	6	7	0	0	0	0	0	0	0	0	4	3	3	0	0	0	3	0	6	6	0	3	5	43	5	0	0	0
+LFQ	211	0	0	0	0	0	0	0	0	0	0	0	0	2	5	6	4	0	0	0	0	0	0	0	0	6	3	5	0	0	0	0	2	5	11	4	3	3	35	6	0	0	0
+LFQ	212	0	0	0	0	0	0	0	0	0	0	0	0	0	3	6	7	0	0	0	0	0	0	0	0	3	4	8	0	0	0	0	0	5	17	1	1	4	35	6	0	0	0
+LFQ	213	0	0	0	0	0	0	0	0	0	0	0	0	6	6	10	5	0	0	0	0	0	0	0	0	5	3	1	0	0	0	0	1	4	7	1	5	2	43	1	0	0	0
+LFQ	214	0	0	0	0	0	0	0	0	0	0	0	0	4	9	4	10	0	0	0	0	0	0	0	0	4	1	6	0	2	0	1	1	3	9	0	2	3	39	2	0	0	0
+LFQ	215	0	0	0	0	0	0	0	0	0	0	0	0	5	6	5	6	0	0	0	0	0	0	0	0	5	5	8	0	0	0	1	2	5	12	0	0	4	34	2	0	0	0
+LFQ	216	0	0	0	0	0	0	0	0	0	0	0	0	3	3	6	5	0	0	0	0	0	0	0	0	3	6	5	1	3	0	1	0	5	15	1	3	2	37	1	0	0	0
+LFQ	217	0	0	0	0	0	0	0	0	0	0	0	0	3	7	6	4	0	0	0	0	0	0	0	0	4	1	2	0	0	1	1	0	10	15	1	3	2	39	1	0	0	0
+LFQ	218	0	0	0	0	0	0	0	0	0	0	0	0	2	4	7	2	0	0	0	0	0	0	0	0	6	4	10	0	0	0	1	0	3	13	1	5	3	35	4	0	0	0
+LFQ	219	0	0	0	0	0	0	0	0	0	0	0	0	2	5	4	4	0	0	0	0	0	0	0	0	5	1	5	0	0	1	0	0	4	13	1	6	3	45	1	0	0	0
+LFQ	220	0	0	0	0	0	0	0	0	0	0	0	0	2	4	9	4	0	0	0	0	0	0	0	0	4	2	2	0	0	0	0	2	5	14	1	1	2	47	1	0	0	0
+LFQ	221	0	0	0	0	0	0	0	0	0	0	0	0	3	4	6	4	0	0	0	0	0	0	0	0	1	4	6	0	0	0	0	0	2	10	1	3	3	53	0	0	0	0
+LFQ	222	0	0	0	0	0	0	0	0	0	0	0	0	3	5	8	6	0	0	0	0	0	0	0	0	5	2	6	0	0	0	0	0	2	6	1	3	4	49	0	0	0	0
+LFQ	223	0	0	0	0	0	0	0	0	0	0	0	0	5	8	6	8	0	0	0	0	0	0	0	0	3	3	5	1	1	0	1	0	0	13	1	0	2	43	0	0	0	0
+LFQ	224	0	0	0	0	0	0	0	0	0	0	0	0	2	9	11	6	0	0	0	0	0	0	0	0	4	3	3	0	0	0	0	1	2	15	1	0	1	42	0	0	0	0
+LFQ	225	0	0	0	0	0	0	0	0	0	0	0	0	4	8	2	4	0	0	0	0	0	0	0	0	7	3	10	0	2	0	0	0	3	10	1	2	5	39	0	0	0	0
+LFQ	226	0	0	0	0	0	0	0	0	0	0	0	0	5	9	3	7	0	0	0	0	0	0	0	0	8	5	5	0	1	0	2	1	3	13	0	2	3	33	0	0	0	0
+LFQ	227	0	0	0	0	0	0	0	0	0	0	0	0	10	8	4	5	0	0	0	0	0	0	0	0	3	2	3	0	0	0	2	3	5	17	0	3	2	33	0	0	0	0
+LFQ	228	0	0	0	0	0	0	0	0	0	0	0	0	10	8	6	2	0	0	0	0	0	0	0	0	6	1	6	0	1	2	1	1	4	13	1	2	1	35	0	0	0	0
+LFQ	229	0	0	0	0	0	0	0	0	0	0	0	0	4	11	6	6	0	0	0	0	0	0	0	0	1	3	5	0	0	0	0	2	6	13	0	3	5	35	0	0	0	0
+LFQ	230	0	0	0	0	0	0	0	0	0	0	0	0	4	11	10	7	0	0	0	0	0	0	0	0	2	3	4	0	0	0	1	2	9	10	0	1	1	35	0	0	0	0
+LFQ	231	0	0	0	0	0	0	0	0	0	0	0	0	6	7	6	6	0	0	0	0	0	0	0	0	4	5	4	0	1	0	1	0	4	13	1	5	5	32	0	0	0	0
+LFQ	232	0	0	0	0	0	0	0	0	0	0	0	0	3	7	10	4	0	0	0	0	0	0	0	0	6	4	7	0	0	0	0	0	3	12	0	1	3	40	0	0	0	0
+LFQ	233	0	0	0	0	0	0	0	0	0	0	0	0	7	7	9	8	0	0	0	0	0	0	0	0	5	5	3	1	1	0	0	0	6	10	1	1	4	32	0	0	0	0
+LFQ	234	0	0	0	0	0	0	0	0	0	0	0	0	9	6	10	5	0	0	0	0	0	0	0	0	5	5	3	1	0	0	0	1	5	11	0	1	4	34	0	0	0	0
+LFQ	235	0	0	0	0	0	0	0	0	0	0	0	0	4	10	13	7	0	0	0	0	0	0	0	0	5	1	4	0	0	0	2	1	5	7	0	0	4	37	0	0	0	0
+LFQ	236	0	0	0	0	0	0	0	0	0	0	0	0	7	8	15	6	0	0	0	0	0	0	0	0	7	3	5	1	0	0	0	1	6	8	0	1	1	31	0	0	0	0
+LFQ	237	0	0	0	0	0	0	0	0	0	0	0	0	2	9	13	6	0	0	0	0	0	0	0	0	6	4	7	1	0	0	0	1	3	12	0	1	1	34	0	0	0	0
+LFQ	238	0	0	0	0	0	0	0	0	0	0	0	0	2	10	16	6	0	0	0	0	0	0	0	0	9	2	3	0	0	0	0	0	5	9	0	2	3	33	0	0	0	0
+LFQ	239	0	0	0	0	0	0	0	0	0	0	0	0	4	5	17	9	0	0	0	0	0	0	0	0	7	3	1	0	0	0	1	1	2	15	0	1	3	31	0	0	0	0
+LFQ	240	0	0	0	0	0	0	0	0	0	0	0	0	0	10	19	6	0	0	0	0	0	0	0	0	6	4	4	0	0	1	3	0	2	13	0	1	1	30	0	0	0	0
+LFQ	241	0	0	0	0	0	0	0	0	0	0	0	0	1	4	23	9	0	0	0	0	0	0	0	0	7	5	8	1	0	0	2	1	1	8	0	1	3	26	0	0	0	0
+LFQ	242	0	0	0	0	0	0	0	0	0	0	0	0	3	10	19	10	0	0	0	0	0	0	0	0	2	5	4	1	0	0	0	1	2	10	0	0	2	31	0	0	0	0
+LFQ	243	0	0	0	0	0	0	0	0	0	0	0	0	2	2	17	14	0	0	0	0	0	0	0	0	6	5	5	0	0	0	0	0	6	13	0	2	3	25	0	0	0	0
+LFQ	244	0	0	0	0	0	0	0	0	0	0	0	0	1	3	18	11	0	0	0	0	0	0	0	0	6	5	5	1	0	0	1	0	2	12	0	5	1	29	0	0	0	0
+LFQ	245	0	0	0	0	0	0	0	0	0	0	0	0	1	8	14	11	0	0	0	0	0	0	0	0	8	5	3	1	0	0	1	0	1	15	1	1	3	27	0	0	0	0
+LFQ	246	0	0	0	0	0	0	0	0	0	0	0	0	4	6	15	12	0	0	0	0	0	0	0	0	5	2	8	1	0	1	1	0	1	13	0	2	1	28	0	0	0	0
+LFQ	247	0	0	0	0	0	0	0	0	0	0	0	0	8	3	16	14	0	0	0	0	0	0	0	0	10	4	6	0	0	0	0	0	2	15	1	2	2	17	0	0	0	0
+LFQ	248	0	0	0	0	0	0	0	0	0	0	0	0	5	6	21	8	0	0	0	0	0	0	0	0	7	8	6	2	0	0	0	1	1	14	0	2	2	17	0	0	0	0
+LFQ	249	0	0	0	0	0	0	0	0	0	0	0	0	5	6	20	8	0	0	0	0	0	0	0	0	5	7	10	0	1	0	0	0	1	15	0	2	0	20	0	0	0	0
+LFQ	250	0	0	0	0	0	0	0	0	0	0	0	0	2	10	15	10	0	0	0	0	0	0	0	0	9	5	8	1	0	0	0	2	6	10	0	0	2	20	0	0	0	0
+LFQ	251	0	0	0	0	0	0	0	0	0	0	0	0	7	20	21	17	0	0	0	0	0	0	0	0	7	7	6	0	0	0	0	1	2	4	0	0	0	8	0	0	0	0
+LFQ	252	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+# Mismatches per cycle and quality. Use `grep ^MPC | cut -f 2-` to extract this part.
+# Columns correspond to qualities, rows to cycles. First column is the cycle number, second
+# is the number of N's and the rest is the number of mismatches
+MPC	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	2	0	0	0	0	0	0
+MPC	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	1	0	0	0	0	0	0
+MPC	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	2	0	0	0	0	0	0
+MPC	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	2	0	0	0	0	0	0
+MPC	5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	7	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	8	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	9	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	10	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	11	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	12	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	13	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	14	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	15	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	16	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	17	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	18	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	19	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	20	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	21	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	22	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	23	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	24	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	25	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	26	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	27	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	28	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	29	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	30	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	31	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	32	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
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+MPC	208	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	209	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	210	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	211	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	212	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	213	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	214	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	215	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	216	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	217	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	218	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	219	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	220	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	221	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	222	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	223	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	224	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	225	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	226	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	227	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	228	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	229	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	230	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	231	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	232	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0
+MPC	233	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
+MPC	234	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
+MPC	235	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	236	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
+MPC	237	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
+MPC	238	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	239	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
+MPC	240	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	241	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	242	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
+MPC	243	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	244	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	245	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	246	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	247	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	1	0	0	0
+MPC	248	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	3	2	0	0	0	0	0	0	0	0	2	1	0	0	0	0	0	0	0	2	0	0	0	3	0	0	0
+MPC	249	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	1	1	0	0	0	0	0	0	0	0	2	0	5	0	0	0	0	0	0	2	0	0	0	0	0	0	0
+MPC	250	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	2	2	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	1	2	0	0	0	2	0	0	0
+MPC	251	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	2	2	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	2	0	0	0	0	3	0	0	0
+MPC	252	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+# GC Content of first fragments. Use `grep ^GCF | cut -f 2-` to extract this part.
+GCF	21.86	0
+GCF	44.22	2
+GCF	44.97	8
+GCF	45.48	4
+GCF	45.98	3
+GCF	46.48	5
+GCF	46.98	2
+GCF	47.49	3
+GCF	47.99	12
+GCF	48.49	13
+GCF	48.99	9
+GCF	49.50	2
+GCF	50.25	3
+GCF	51.01	6
+GCF	51.51	4
+GCF	52.01	1
+GCF	52.51	0
+GCF	53.02	1
+# GC Content of last fragments. Use `grep ^GCL | cut -f 2-` to extract this part.
+GCL	21.86	0
+GCL	44.22	2
+GCL	44.97	8
+GCL	45.48	4
+GCL	45.98	3
+GCL	46.48	5
+GCL	46.98	2
+GCL	47.49	3
+GCL	47.99	12
+GCL	48.49	13
+GCL	48.99	9
+GCL	49.50	2
+GCL	50.25	3
+GCL	51.01	6
+GCL	51.51	4
+GCL	52.01	1
+GCL	52.51	0
+GCL	53.02	1
+# ACGT content per cycle. Use `grep ^GCC | cut -f 2-` to extract this part. The columns are: cycle, and A,C,G,T counts [%]
+GCC	1	21.50	29.50	33.50	15.50
+GCC	2	30.00	16.00	11.00	43.00
+GCC	3	33.00	25.00	9.00	33.00
+GCC	4	17.00	29.00	13.00	41.00
+GCC	5	37.00	22.00	12.00	29.00
+GCC	6	36.00	26.00	17.00	21.00
+GCC	7	29.50	13.50	31.50	25.50
+GCC	8	50.50	14.50	19.50	15.50
+GCC	9	17.00	33.00	18.00	32.00
+GCC	10	37.00	14.00	21.00	28.00
+GCC	11	20.50	14.50	29.50	35.50
+GCC	12	30.00	24.00	22.00	24.00
+GCC	13	27.00	15.00	21.00	37.00
+GCC	14	24.00	22.00	26.00	28.00
+GCC	15	25.50	20.50	24.50	29.50
+GCC	16	31.00	15.00	20.00	34.00
+GCC	17	28.00	25.00	14.00	33.00
+GCC	18	30.50	28.50	19.50	21.50
+GCC	19	29.00	26.00	21.00	24.00
+GCC	20	22.50	23.50	17.50	36.50
+GCC	21	35.50	17.50	19.50	27.50
+GCC	22	37.50	28.50	15.50	18.50
+GCC	23	31.00	19.00	13.00	37.00
+GCC	24	37.00	12.00	22.00	29.00
+GCC	25	35.50	22.50	17.50	24.50
+GCC	26	33.50	18.50	15.50	32.50
+GCC	27	34.50	14.50	25.50	25.50
+GCC	28	31.00	14.00	24.00	31.00
+GCC	29	30.00	27.00	24.00	19.00
+GCC	30	31.00	20.00	14.00	35.00
+GCC	31	33.50	29.50	13.50	23.50
+GCC	32	42.50	20.50	19.50	17.50
+GCC	33	25.50	23.50	14.50	36.50
+GCC	34	39.50	16.50	20.50	23.50
+GCC	35	32.50	23.50	21.50	22.50
+GCC	36	42.00	25.00	16.00	17.00
+GCC	37	38.00	17.00	19.00	26.00
+GCC	38	24.00	26.00	25.00	25.00
+GCC	39	22.50	41.50	18.50	17.50
+GCC	40	32.00	16.00	21.00	31.00
+GCC	41	33.00	28.00	19.00	20.00
+GCC	42	30.50	25.50	19.50	24.50
+GCC	43	35.00	29.00	15.00	21.00
+GCC	44	20.00	27.00	22.00	31.00
+GCC	45	40.50	21.50	21.50	16.50
+GCC	46	26.50	20.50	22.50	30.50
+GCC	47	38.50	29.50	16.50	15.50
+GCC	48	27.50	24.50	17.50	30.50
+GCC	49	28.50	32.50	10.50	28.50
+GCC	50	46.50	20.50	9.50	23.50
+GCC	51	34.50	28.50	13.50	23.50
+GCC	52	41.50	23.50	20.50	14.50
+GCC	53	20.00	28.00	26.00	26.00
+GCC	54	31.50	18.50	24.50	25.50
+GCC	55	30.50	22.50	16.50	30.50
+GCC	56	33.50	22.50	13.50	30.50
+GCC	57	23.00	24.00	23.00	30.00
+GCC	58	25.00	37.00	19.00	19.00
+GCC	59	34.00	23.00	24.00	19.00
+GCC	60	29.00	28.00	17.00	26.00
+GCC	61	25.50	23.50	24.50	26.50
+GCC	62	31.50	22.50	16.50	29.50
+GCC	63	27.50	28.50	25.50	18.50
+GCC	64	33.50	21.50	25.50	19.50
+GCC	65	35.50	19.50	18.50	26.50
+GCC	66	34.00	25.00	15.00	26.00
+GCC	67	37.00	23.00	19.00	21.00
+GCC	68	36.50	29.50	13.50	20.50
+GCC	69	38.50	19.50	20.50	21.50
+GCC	70	38.50	16.50	18.50	26.50
+GCC	71	25.50	38.50	21.50	14.50
+GCC	72	29.00	29.00	25.00	17.00
+GCC	73	32.50	20.50	21.50	25.50
+GCC	74	28.50	32.50	12.50	26.50
+GCC	75	41.50	12.50	18.50	27.50
+GCC	76	24.50	29.50	23.50	22.50
+GCC	77	36.00	21.00	18.00	25.00
+GCC	78	27.00	34.00	22.00	17.00
+GCC	79	21.50	26.50	25.50	26.50
+GCC	80	34.00	19.00	28.00	19.00
+GCC	81	17.00	26.00	26.00	31.00
+GCC	82	31.00	30.00	23.00	16.00
+GCC	83	31.50	26.50	12.50	29.50
+GCC	84	19.00	41.00	21.00	19.00
+GCC	85	37.50	24.50	16.50	21.50
+GCC	86	15.00	48.00	15.00	22.00
+GCC	87	41.00	16.00	18.00	25.00
+GCC	88	23.50	27.50	27.50	21.50
+GCC	89	26.50	27.50	26.50	19.50
+GCC	90	18.50	23.50	24.50	33.50
+GCC	91	27.00	32.00	22.00	19.00
+GCC	92	23.50	17.50	27.50	31.50
+GCC	93	25.50	37.50	15.50	21.50
+GCC	94	27.00	17.00	24.00	32.00
+GCC	95	26.50	37.50	14.50	21.50
+GCC	96	29.50	25.50	16.50	28.50
+GCC	97	29.00	31.00	21.00	19.00
+GCC	98	18.00	33.00	22.00	27.00
+GCC	99	24.50	33.50	24.50	17.50
+GCC	100	24.50	16.50	24.50	34.50
+GCC	101	25.00	40.00	19.00	16.00
+GCC	102	17.50	17.50	32.50	32.50
+GCC	103	31.00	26.00	16.00	27.00
+GCC	104	26.50	29.50	20.50	23.50
+GCC	105	34.00	33.00	21.00	12.00
+GCC	106	23.00	31.00	26.00	20.00
+GCC	107	17.50	35.50	23.50	23.50
+GCC	108	24.50	30.50	23.50	21.50
+GCC	109	17.00	31.00	22.00	30.00
+GCC	110	16.00	35.00	24.00	25.00
+GCC	111	24.00	32.00	23.00	21.00
+GCC	112	37.00	28.00	16.00	19.00
+GCC	113	19.50	22.50	32.50	25.50
+GCC	114	17.00	31.00	35.00	17.00
+GCC	115	29.50	24.50	23.50	22.50
+GCC	116	22.00	30.00	34.00	14.00
+GCC	117	27.00	23.00	19.00	31.00
+GCC	118	25.50	14.50	34.50	25.50
+GCC	119	22.50	34.50	20.50	22.50
+GCC	120	17.50	24.50	26.50	31.50
+GCC	121	27.50	33.50	22.50	16.50
+GCC	122	17.00	23.00	25.00	35.00
+GCC	123	23.50	46.50	11.50	18.50
+GCC	124	9.00	32.00	34.00	25.00
+GCC	125	24.00	27.00	19.00	30.00
+GCC	126	26.00	17.00	28.00	29.00
+GCC	127	26.50	16.50	21.50	35.50
+GCC	128	18.00	34.00	31.00	17.00
+GCC	129	25.50	25.50	27.50	21.50
+GCC	130	25.00	20.00	22.00	33.00
+GCC	131	17.50	39.50	24.50	18.50
+GCC	132	21.00	28.00	23.00	28.00
+GCC	133	13.50	31.50	35.50	19.50
+GCC	134	24.50	19.50	30.50	25.50
+GCC	135	16.50	23.50	30.50	29.50
+GCC	136	28.00	32.00	15.00	25.00
+GCC	137	22.50	21.50	30.50	25.50
+GCC	138	14.50	34.50	24.50	26.50
+GCC	139	20.50	29.50	24.50	25.50
+GCC	140	17.00	23.00	30.00	30.00
+GCC	141	20.50	23.50	25.50	30.50
+GCC	142	18.00	29.00	38.00	15.00
+GCC	143	22.00	24.00	27.00	27.00
+GCC	144	21.50	30.50	26.50	21.50
+GCC	145	22.00	21.00	29.00	28.00
+GCC	146	25.00	16.00	39.00	20.00
+GCC	147	26.50	22.50	30.50	20.50
+GCC	148	12.50	28.50	36.50	22.50
+GCC	149	26.50	23.50	23.50	26.50
+GCC	150	14.00	29.00	24.00	33.00
+GCC	151	19.50	30.50	32.50	17.50
+GCC	152	18.50	17.50	29.50	34.50
+GCC	153	22.50	22.50	31.50	23.50
+GCC	154	22.00	21.00	29.00	28.00
+GCC	155	21.00	26.00	19.00	34.00
+GCC	156	14.50	23.50	35.50	26.50
+GCC	157	22.00	31.00	23.00	24.00
+GCC	158	22.50	29.50	24.50	23.50
+GCC	159	17.50	12.50	46.50	23.50
+GCC	160	24.50	26.50	26.50	22.50
+GCC	161	13.00	23.00	45.00	19.00
+GCC	162	31.50	16.50	22.50	29.50
+GCC	163	19.50	21.50	35.50	23.50
+GCC	164	29.00	18.00	21.00	32.00
+GCC	165	14.50	17.50	35.50	32.50
+GCC	166	17.50	37.50	28.50	16.50
+GCC	167	16.50	21.50	31.50	30.50
+GCC	168	14.00	25.00	30.00	31.00
+GCC	169	18.50	20.50	23.50	37.50
+GCC	170	19.00	23.00	28.00	30.00
+GCC	171	20.00	28.00	33.00	19.00
+GCC	172	19.00	20.00	32.00	29.00
+GCC	173	24.50	16.50	33.50	25.50
+GCC	174	17.50	23.50	33.50	25.50
+GCC	175	33.50	17.50	35.50	13.50
+GCC	176	16.50	32.50	28.50	22.50
+GCC	177	19.00	22.00	27.00	32.00
+GCC	178	16.00	26.00	30.00	28.00
+GCC	179	18.00	18.00	22.00	42.00
+GCC	180	21.00	22.00	34.00	23.00
+GCC	181	20.50	19.50	35.50	24.50
+GCC	182	32.50	18.50	22.50	26.50
+GCC	183	24.50	13.50	28.50	33.50
+GCC	184	15.00	29.00	30.00	26.00
+GCC	185	15.00	32.00	33.00	20.00
+GCC	186	22.50	23.50	34.50	19.50
+GCC	187	19.00	14.00	40.00	27.00
+GCC	188	27.50	21.50	27.50	23.50
+GCC	189	17.00	22.00	34.00	27.00
+GCC	190	23.00	30.00	23.00	24.00
+GCC	191	25.00	22.00	28.00	25.00
+GCC	192	34.50	24.50	13.50	27.50
+GCC	193	18.50	25.50	25.50	30.50
+GCC	194	18.50	33.50	24.50	23.50
+GCC	195	16.00	26.00	23.00	35.00
+GCC	196	21.50	25.50	24.50	28.50
+GCC	197	20.00	21.00	23.00	36.00
+GCC	198	17.00	21.00	37.00	25.00
+GCC	199	20.50	18.50	25.50	35.50
+GCC	200	21.00	29.00	21.00	29.00
+GCC	201	27.00	21.00	23.00	29.00
+GCC	202	21.50	24.50	19.50	34.50
+GCC	203	21.50	24.50	26.50	27.50
+GCC	204	27.00	29.00	24.00	20.00
+GCC	205	19.50	21.50	22.50	36.50
+GCC	206	26.50	24.50	21.50	27.50
+GCC	207	22.50	21.50	19.50	36.50
+GCC	208	14.00	35.00	29.00	22.00
+GCC	209	16.00	23.00	12.00	49.00
+GCC	210	18.50	19.50	40.50	21.50
+GCC	211	26.00	20.00	22.00	32.00
+GCC	212	21.00	31.00	18.00	30.00
+GCC	213	24.00	15.00	31.00	30.00
+GCC	214	17.50	24.50	25.50	32.50
+GCC	215	26.00	24.00	23.00	27.00
+GCC	216	21.50	17.50	25.50	35.50
+GCC	217	26.00	29.00	17.00	28.00
+GCC	218	20.00	27.00	21.00	32.00
+GCC	219	17.00	21.00	21.00	41.00
+GCC	220	25.50	23.50	23.50	27.50
+GCC	221	21.50	23.50	20.50	34.50
+GCC	222	21.50	21.50	18.50	38.50
+GCC	223	20.00	27.00	28.00	25.00
+GCC	224	22.50	22.50	24.50	30.50
+GCC	225	14.50	35.50	30.50	19.50
+GCC	226	20.00	23.00	26.00	31.00
+GCC	227	20.50	24.50	23.50	31.50
+GCC	228	33.00	19.00	26.00	22.00
+GCC	229	22.50	24.50	18.50	34.50
+GCC	230	21.00	32.00	16.00	31.00
+GCC	231	23.00	28.00	30.00	19.00
+GCC	232	23.50	21.50	12.50	42.50
+GCC	233	21.00	27.00	25.00	27.00
+GCC	234	16.50	27.50	22.50	33.50
+GCC	235	20.00	15.00	28.00	37.00
+GCC	236	28.00	23.00	21.00	28.00
+GCC	237	20.50	19.50	22.50	37.50
+GCC	238	21.50	29.50	24.50	24.50
+GCC	239	20.00	8.00	17.00	55.00
+GCC	240	28.00	24.00	16.00	32.00
+GCC	241	22.50	22.50	16.50	38.50
+GCC	242	29.00	25.00	13.00	33.00
+GCC	243	22.50	15.50	23.50	38.50
+GCC	244	20.50	23.50	16.50	39.50
+GCC	245	28.00	23.00	19.00	30.00
+GCC	246	21.00	29.00	25.00	25.00
+GCC	247	32.00	14.00	13.00	41.00
+GCC	248	18.00	18.00	25.00	39.00
+GCC	249	25.00	23.00	21.00	31.00
+GCC	250	27.50	22.50	17.50	32.50
+GCC	251	13.50	20.50	36.50	29.50
+# Insert sizes. Use `grep ^IS | cut -f 2-` to extract this part. The columns are: insert size, pairs total, inward oriented pairs, outward oriented pairs, other pairs
+# Read lengths. Use `grep ^RL | cut -f 2-` to extract this part. The columns are: read length, count
+RL	251	200
+# Indel distribution. Use `grep ^ID | cut -f 2-` to extract this part. The columns are: length, number of insertions, number of deletions
+ID	1	1	0
+ID	2	1	0
+ID	4	2	0
+ID	10	5	0
+ID	13	1	0
+ID	14	1	0
+ID	15	1	0
+ID	18	1	0
+ID	21	1	0
+ID	22	1	0
+ID	23	2	0
+ID	24	3	0
+ID	29	1	0
+ID	35	2	0
+ID	38	2	0
+# Indels per cycle. Use `grep ^IC | cut -f 2-` to extract this part. The columns are: cycle, number of insertions (fwd), .. (rev) , number of deletions (fwd), .. (rev)
+IC	2	1	0	0	0
+IC	4	2	0	0	0
+IC	5	3	0	0	0
+IC	210	2	0	0	0
+IC	219	1	0	0	0
+IC	220	1	0	0	0
+IC	224	2	0	0	0
+IC	225	2	0	0	0
+IC	226	1	0	0	0
+IC	228	1	0	0	0
+IC	230	1	0	0	0
+IC	233	1	0	0	0
+IC	234	1	0	0	0
+IC	235	1	0	0	0
+IC	236	1	0	0	0
+IC	239	1	0	0	0
+IC	240	1	0	0	0
+IC	241	1	0	0	0
+IC	247	1	0	0	0
+# Coverage distribution. Use `grep ^COV | cut -f 2-` to extract this part.
+COV	[1-1]	1	1
+COV	[7-7]	7	1
+COV	[18-18]	18	1
+COV	[24-24]	24	1
+COV	[25-25]	25	249
+# GC-depth. Use `grep ^GCD | cut -f 2-` to extract this part. The columns are: GC%, unique sequence percentiles, 10th, 25th, 50th, 75th and 90th depth percentile
+GCD	0.0	100.000	0.000	0.000	0.000	0.000	0.000
diff --git a/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2.tab b/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2.tab
new file mode 100644
index 00000000000..7867a751100
--- /dev/null
+++ b/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2.tab
@@ -0,0 +1,1137 @@
+# This file was produced by samtools stats (1.2+htslib-1.2.1) and can be plotted using plot-bamstats
+# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /Users/anton/galaxy-git/database/files/000/dataset_9.dat /Users/anton/galaxy-git/database/files/000/dataset_10.dat
+# CHK, Checksum	[2]Read Names	[3]Sequences	[4]Qualities
+# CHK, CRC32 of reads which passed filtering followed by addition (32bit overflow)
+CHK	1bd20fd8	58ad2167	29883386
+# Summary Numbers. Use `grep ^SN | cut -f 2-` to extract this part.
+SN	raw total sequences:	200
+SN	filtered sequences:	0
+SN	sequences:	200
+SN	is sorted:	1
+SN	1st fragments:	100
+SN	last fragments:	100
+SN	reads mapped:	25
+SN	reads mapped and paired:	0	# paired-end technology bit set + both mates mapped
+SN	reads unmapped:	175
+SN	reads properly paired:	0	# proper-pair bit set
+SN	reads paired:	200	# paired-end technology bit set
+SN	reads duplicated:	0	# PCR or optical duplicate bit set
+SN	reads MQ0:	6	# mapped and MQ=0
+SN	reads QC failed:	0
+SN	non-primary alignments:	0
+SN	total length:	50200	# ignores clipping
+SN	bases mapped:	6275	# ignores clipping
+SN	bases mapped (cigar):	6275	# more accurate
+SN	bases trimmed:	0
+SN	bases duplicated:	0
+SN	mismatches:	591	# from NM fields
+SN	error rate:	9.418327e-02	# mismatches / bases mapped (cigar)
+SN	average length:	251
+SN	maximum length:	251
+SN	average quality:	34.7
+SN	insert size average:	0.0
+SN	insert size standard deviation:	0.0
+SN	inward oriented pairs:	0
+SN	outward oriented pairs:	0
+SN	pairs with other orientation:	0
+SN	pairs on different chromosomes:	0
+# First Fragment Qualitites. Use `grep ^FFQ | cut -f 2-` to extract this part.
+# Columns correspond to qualities and rows to cycles. First column is the cycle number.
+FFQ	1	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	3	2	0	20	38	32	1	0	0	0	0	0	0
+FFQ	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	3	0	0	18	36	36	6	0	0	0	0	0	0
+FFQ	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	2	1	0	14	31	46	5	0	0	0	0	0	0
+FFQ	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	8	36	42	11	0	0	0	0	0	0
+FFQ	5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	2	0	0	0	0	0	0	0	0	0	0	1	0	0	12	35	43	6	0	0	0	0	0	0
+FFQ	6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	1	7	8	0	0	81	0	0	0	0
+FFQ	7	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	9	13	1	0	76	0	0	0	0
+FFQ	8	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	11	15	4	0	69	0	0	0	0
+FFQ	9	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	6	20	3	0	68	0	0	0	0
+FFQ	10	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	7	11	5	0	75	0	0	0	0
+FFQ	11	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	6	16	6	0	70	0	0	0	0
+FFQ	12	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	2	8	2	0	86	0	0	0	0
+FFQ	13	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	3	92	0	0	0
+FFQ	14	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	2	2	1	0	3	89	0	0	0
+FFQ	15	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	2	4	91	0	0	0
+FFQ	16	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	2	96	0	0	0
+FFQ	17	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	3	94	0	0	0
+FFQ	18	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	1	0	2	0	0	2	93	0	0	0
+FFQ	19	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	4	93	0	0	0
+FFQ	20	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	2	1	4	91	0	0	0
+FFQ	21	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	3	94	0	0	0
+FFQ	22	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	97	0	0	0
+FFQ	23	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	4	18	74	0	0
+FFQ	24	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	1	1	19	76	0	0
+FFQ	25	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	1	0	0	4	15	76	0	0
+FFQ	26	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	2	2	18	75	0	0
+FFQ	27	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	12	84	0	0
+FFQ	28	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	2	5	16	74	0	0
+FFQ	29	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	6	19	73	0	0
+FFQ	30	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	1	0	12	17	67	0	0
+FFQ	31	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	1	1	0	1	2	15	77	0	0
+FFQ	32	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	7	22	68	0	0
+FFQ	33	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	2	0	0	1	3	18	73	0	0
+FFQ	34	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	7	19	72	0	0
+FFQ	35	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	2	0	0	2	23	71	0	0
+FFQ	36	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	2	26	69	0	0
+FFQ	37	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	3	0	6	23	65	0	0
+FFQ	38	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	3	31	63	0	0
+FFQ	39	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	4	5	33	56	0	0
+FFQ	40	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	1	0	0	0	0	0	0	0	0	1	0	0	0	0	3	1	1	7	25	60	0	0
+FFQ	41	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	1	0	0	0	0	0	0	0	0	1	0	0	1	0	1	0	1	7	19	67	0	0
+FFQ	42	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	1	7	21	68	0	0
+FFQ	43	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	1	4	5	28	56	0	0
+FFQ	44	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	1	1	1	2	13	29	49	0	0
+FFQ	45	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	1	1	0	0	0	7	31	57	0	0
+FFQ	46	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	4	2	8	34	49	0	0
+FFQ	47	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	3	1	1	0	8	22	63	0	0
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+FFQ	226	0	0	0	0	0	0	0	0	0	0	0	0	5	9	3	7	0	0	0	0	0	0	0	0	8	5	5	0	1	0	2	1	3	13	0	2	3	33	0	0	0	0
+FFQ	227	0	0	0	0	0	0	0	0	0	0	0	0	10	8	4	5	0	0	0	0	0	0	0	0	3	2	3	0	0	0	2	3	5	17	0	3	2	33	0	0	0	0
+FFQ	228	0	0	0	0	0	0	0	0	0	0	0	0	10	8	6	2	0	0	0	0	0	0	0	0	6	1	6	0	1	2	1	1	4	13	1	2	1	35	0	0	0	0
+FFQ	229	0	0	0	0	0	0	0	0	0	0	0	0	4	11	6	6	0	0	0	0	0	0	0	0	1	3	5	0	0	0	0	2	6	13	0	3	5	35	0	0	0	0
+FFQ	230	0	0	0	0	0	0	0	0	0	0	0	0	4	11	10	7	0	0	0	0	0	0	0	0	2	3	4	0	0	0	1	2	9	10	0	1	1	35	0	0	0	0
+FFQ	231	0	0	0	0	0	0	0	0	0	0	0	0	6	7	6	6	0	0	0	0	0	0	0	0	4	5	4	0	1	0	1	0	4	13	1	5	5	32	0	0	0	0
+FFQ	232	0	0	0	0	0	0	0	0	0	0	0	0	3	7	10	4	0	0	0	0	0	0	0	0	6	4	7	0	0	0	0	0	3	12	0	1	3	40	0	0	0	0
+FFQ	233	0	0	0	0	0	0	0	0	0	0	0	0	7	7	9	8	0	0	0	0	0	0	0	0	5	5	3	1	1	0	0	0	6	10	1	1	4	32	0	0	0	0
+FFQ	234	0	0	0	0	0	0	0	0	0	0	0	0	9	6	10	5	0	0	0	0	0	0	0	0	5	5	3	1	0	0	0	1	5	11	0	1	4	34	0	0	0	0
+FFQ	235	0	0	0	0	0	0	0	0	0	0	0	0	4	10	13	7	0	0	0	0	0	0	0	0	5	1	4	0	0	0	2	1	5	7	0	0	4	37	0	0	0	0
+FFQ	236	0	0	0	0	0	0	0	0	0	0	0	0	7	8	15	6	0	0	0	0	0	0	0	0	7	3	5	1	0	0	0	1	6	8	0	1	1	31	0	0	0	0
+FFQ	237	0	0	0	0	0	0	0	0	0	0	0	0	2	9	13	6	0	0	0	0	0	0	0	0	6	4	7	1	0	0	0	1	3	12	0	1	1	34	0	0	0	0
+FFQ	238	0	0	0	0	0	0	0	0	0	0	0	0	2	10	16	6	0	0	0	0	0	0	0	0	9	2	3	0	0	0	0	0	5	9	0	2	3	33	0	0	0	0
+FFQ	239	0	0	0	0	0	0	0	0	0	0	0	0	4	5	17	9	0	0	0	0	0	0	0	0	7	3	1	0	0	0	1	1	2	15	0	1	3	31	0	0	0	0
+FFQ	240	0	0	0	0	0	0	0	0	0	0	0	0	0	10	19	6	0	0	0	0	0	0	0	0	6	4	4	0	0	1	3	0	2	13	0	1	1	30	0	0	0	0
+FFQ	241	0	0	0	0	0	0	0	0	0	0	0	0	1	4	23	9	0	0	0	0	0	0	0	0	7	5	8	1	0	0	2	1	1	8	0	1	3	26	0	0	0	0
+FFQ	242	0	0	0	0	0	0	0	0	0	0	0	0	3	10	19	10	0	0	0	0	0	0	0	0	2	5	4	1	0	0	0	1	2	10	0	0	2	31	0	0	0	0
+FFQ	243	0	0	0	0	0	0	0	0	0	0	0	0	2	2	17	14	0	0	0	0	0	0	0	0	6	5	5	0	0	0	0	0	6	13	0	2	3	25	0	0	0	0
+FFQ	244	0	0	0	0	0	0	0	0	0	0	0	0	1	3	18	11	0	0	0	0	0	0	0	0	6	5	5	1	0	0	1	0	2	12	0	5	1	29	0	0	0	0
+FFQ	245	0	0	0	0	0	0	0	0	0	0	0	0	1	8	14	11	0	0	0	0	0	0	0	0	8	5	3	1	0	0	1	0	1	15	1	1	3	27	0	0	0	0
+FFQ	246	0	0	0	0	0	0	0	0	0	0	0	0	4	6	15	12	0	0	0	0	0	0	0	0	5	2	8	1	0	1	1	0	1	13	0	2	1	28	0	0	0	0
+FFQ	247	0	0	0	0	0	0	0	0	0	0	0	0	8	3	16	14	0	0	0	0	0	0	0	0	10	4	6	0	0	0	0	0	2	15	1	2	2	17	0	0	0	0
+FFQ	248	0	0	0	0	0	0	0	0	0	0	0	0	5	6	21	8	0	0	0	0	0	0	0	0	7	8	6	2	0	0	0	1	1	14	0	2	2	17	0	0	0	0
+FFQ	249	0	0	0	0	0	0	0	0	0	0	0	0	5	6	20	8	0	0	0	0	0	0	0	0	5	7	10	0	1	0	0	0	1	15	0	2	0	20	0	0	0	0
+FFQ	250	0	0	0	0	0	0	0	0	0	0	0	0	2	10	15	10	0	0	0	0	0	0	0	0	9	5	8	1	0	0	0	2	6	10	0	0	2	20	0	0	0	0
+FFQ	251	0	0	0	0	0	0	0	0	0	0	0	0	7	20	21	17	0	0	0	0	0	0	0	0	7	7	6	0	0	0	0	1	2	4	0	0	0	8	0	0	0	0
+FFQ	252	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+# Last Fragment Qualitites. Use `grep ^LFQ | cut -f 2-` to extract this part.
+# Columns correspond to qualities and rows to cycles. First column is the cycle number.
+LFQ	1	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	3	2	0	20	38	32	1	0	0	0	0	0	0
+LFQ	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	3	0	0	18	36	36	6	0	0	0	0	0	0
+LFQ	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	2	1	0	14	31	46	5	0	0	0	0	0	0
+LFQ	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	8	36	42	11	0	0	0	0	0	0
+LFQ	5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	2	0	0	0	0	0	0	0	0	0	0	1	0	0	12	35	43	6	0	0	0	0	0	0
+LFQ	6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	1	7	8	0	0	81	0	0	0	0
+LFQ	7	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	9	13	1	0	76	0	0	0	0
+LFQ	8	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	11	15	4	0	69	0	0	0	0
+LFQ	9	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	6	20	3	0	68	0	0	0	0
+LFQ	10	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	7	11	5	0	75	0	0	0	0
+LFQ	11	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	6	16	6	0	70	0	0	0	0
+LFQ	12	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	2	8	2	0	86	0	0	0	0
+LFQ	13	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	3	92	0	0	0
+LFQ	14	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	2	2	1	0	3	89	0	0	0
+LFQ	15	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	2	4	91	0	0	0
+LFQ	16	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	2	96	0	0	0
+LFQ	17	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	3	94	0	0	0
+LFQ	18	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	1	0	2	0	0	2	93	0	0	0
+LFQ	19	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	4	93	0	0	0
+LFQ	20	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	2	1	4	91	0	0	0
+LFQ	21	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	3	94	0	0	0
+LFQ	22	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	97	0	0	0
+LFQ	23	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	4	18	74	0	0
+LFQ	24	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	1	1	19	76	0	0
+LFQ	25	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	1	0	0	4	15	76	0	0
+LFQ	26	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	2	2	18	75	0	0
+LFQ	27	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	12	84	0	0
+LFQ	28	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	2	5	16	74	0	0
+LFQ	29	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	6	19	73	0	0
+LFQ	30	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	1	0	12	17	67	0	0
+LFQ	31	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	1	1	0	1	2	15	77	0	0
+LFQ	32	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	7	22	68	0	0
+LFQ	33	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	2	0	0	1	3	18	73	0	0
+LFQ	34	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	7	19	72	0	0
+LFQ	35	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	2	0	0	2	23	71	0	0
+LFQ	36	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	2	26	69	0	0
+LFQ	37	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	3	0	6	23	65	0	0
+LFQ	38	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	3	31	63	0	0
+LFQ	39	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	4	5	33	56	0	0
+LFQ	40	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	1	0	0	0	0	0	0	0	0	1	0	0	0	0	3	1	1	7	25	60	0	0
+LFQ	41	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	1	0	0	0	0	0	0	0	0	1	0	0	1	0	1	0	1	7	19	67	0	0
+LFQ	42	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	1	7	21	68	0	0
+LFQ	43	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	1	4	5	28	56	0	0
+LFQ	44	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	1	1	1	2	13	29	49	0	0
+LFQ	45	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	1	1	0	0	0	7	31	57	0	0
+LFQ	46	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	4	2	8	34	49	0	0
+LFQ	47	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	3	1	1	0	8	22	63	0	0
+LFQ	48	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	1	7	24	64	0	0
+LFQ	49	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	9	28	58	0	0
+LFQ	50	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	2	0	1	1	6	12	76	0	0
+LFQ	51	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	4	2	28	63	0	0
+LFQ	52	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	6	27	62	0	0
+LFQ	53	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	2	8	27	58	0	0
+LFQ	54	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	2	21	73	0	0
+LFQ	55	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	4	8	18	68	0	0
+LFQ	56	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	6	9	18	63	0	0
+LFQ	57	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	0	0	1	0	1	9	18	69	0	0
+LFQ	58	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	4	6	17	69	2	0
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+LFQ	237	0	0	0	0	0	0	0	0	0	0	0	0	2	9	13	6	0	0	0	0	0	0	0	0	6	4	7	1	0	0	0	1	3	12	0	1	1	34	0	0	0	0
+LFQ	238	0	0	0	0	0	0	0	0	0	0	0	0	2	10	16	6	0	0	0	0	0	0	0	0	9	2	3	0	0	0	0	0	5	9	0	2	3	33	0	0	0	0
+LFQ	239	0	0	0	0	0	0	0	0	0	0	0	0	4	5	17	9	0	0	0	0	0	0	0	0	7	3	1	0	0	0	1	1	2	15	0	1	3	31	0	0	0	0
+LFQ	240	0	0	0	0	0	0	0	0	0	0	0	0	0	10	19	6	0	0	0	0	0	0	0	0	6	4	4	0	0	1	3	0	2	13	0	1	1	30	0	0	0	0
+LFQ	241	0	0	0	0	0	0	0	0	0	0	0	0	1	4	23	9	0	0	0	0	0	0	0	0	7	5	8	1	0	0	2	1	1	8	0	1	3	26	0	0	0	0
+LFQ	242	0	0	0	0	0	0	0	0	0	0	0	0	3	10	19	10	0	0	0	0	0	0	0	0	2	5	4	1	0	0	0	1	2	10	0	0	2	31	0	0	0	0
+LFQ	243	0	0	0	0	0	0	0	0	0	0	0	0	2	2	17	14	0	0	0	0	0	0	0	0	6	5	5	0	0	0	0	0	6	13	0	2	3	25	0	0	0	0
+LFQ	244	0	0	0	0	0	0	0	0	0	0	0	0	1	3	18	11	0	0	0	0	0	0	0	0	6	5	5	1	0	0	1	0	2	12	0	5	1	29	0	0	0	0
+LFQ	245	0	0	0	0	0	0	0	0	0	0	0	0	1	8	14	11	0	0	0	0	0	0	0	0	8	5	3	1	0	0	1	0	1	15	1	1	3	27	0	0	0	0
+LFQ	246	0	0	0	0	0	0	0	0	0	0	0	0	4	6	15	12	0	0	0	0	0	0	0	0	5	2	8	1	0	1	1	0	1	13	0	2	1	28	0	0	0	0
+LFQ	247	0	0	0	0	0	0	0	0	0	0	0	0	8	3	16	14	0	0	0	0	0	0	0	0	10	4	6	0	0	0	0	0	2	15	1	2	2	17	0	0	0	0
+LFQ	248	0	0	0	0	0	0	0	0	0	0	0	0	5	6	21	8	0	0	0	0	0	0	0	0	7	8	6	2	0	0	0	1	1	14	0	2	2	17	0	0	0	0
+LFQ	249	0	0	0	0	0	0	0	0	0	0	0	0	5	6	20	8	0	0	0	0	0	0	0	0	5	7	10	0	1	0	0	0	1	15	0	2	0	20	0	0	0	0
+LFQ	250	0	0	0	0	0	0	0	0	0	0	0	0	2	10	15	10	0	0	0	0	0	0	0	0	9	5	8	1	0	0	0	2	6	10	0	0	2	20	0	0	0	0
+LFQ	251	0	0	0	0	0	0	0	0	0	0	0	0	7	20	21	17	0	0	0	0	0	0	0	0	7	7	6	0	0	0	0	1	2	4	0	0	0	8	0	0	0	0
+LFQ	252	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+# Mismatches per cycle and quality. Use `grep ^MPC | cut -f 2-` to extract this part.
+# Columns correspond to qualities, rows to cycles. First column is the cycle number, second
+# is the number of N's and the rest is the number of mismatches
+MPC	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	2	0	0	0	0	0	0
+MPC	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	1	0	0	0	0	0	0
+MPC	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	2	0	0	0	0	0	0
+MPC	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	2	0	0	0	0	0	0
+MPC	5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	7	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	8	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	9	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	10	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	11	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	12	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	13	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	14	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	15	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	16	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	17	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	18	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	19	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	20	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	21	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	22	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	23	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	24	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	25	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	26	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	27	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	28	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	29	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	30	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	31	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	32	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	33	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	34	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	35	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	36	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	37	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	38	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	39	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	40	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	41	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	42	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	43	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	44	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	45	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	46	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	47	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	48	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	49	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	50	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	51	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	52	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	53	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	54	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	55	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	56	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	57	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	58	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	59	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	60	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	61	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	62	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	63	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	64	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	65	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	66	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	67	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	68	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	69	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
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+MPC	246	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+MPC	247	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	1	0	0	0
+MPC	248	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	3	2	0	0	0	0	0	0	0	0	2	1	0	0	0	0	0	0	0	2	0	0	0	3	0	0	0
+MPC	249	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	1	1	0	0	0	0	0	0	0	0	2	0	5	0	0	0	0	0	0	2	0	0	0	0	0	0	0
+MPC	250	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	2	2	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	1	2	0	0	0	2	0	0	0
+MPC	251	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	2	2	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	2	0	0	0	0	3	0	0	0
+MPC	252	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+# GC Content of first fragments. Use `grep ^GCF | cut -f 2-` to extract this part.
+GCF	21.86	0
+GCF	44.22	2
+GCF	44.97	8
+GCF	45.48	4
+GCF	45.98	3
+GCF	46.48	5
+GCF	46.98	2
+GCF	47.49	3
+GCF	47.99	12
+GCF	48.49	13
+GCF	48.99	9
+GCF	49.50	2
+GCF	50.25	3
+GCF	51.01	6
+GCF	51.51	4
+GCF	52.01	1
+GCF	52.51	0
+GCF	53.02	1
+# GC Content of last fragments. Use `grep ^GCL | cut -f 2-` to extract this part.
+GCL	21.86	0
+GCL	44.22	2
+GCL	44.97	8
+GCL	45.48	4
+GCL	45.98	3
+GCL	46.48	5
+GCL	46.98	2
+GCL	47.49	3
+GCL	47.99	12
+GCL	48.49	13
+GCL	48.99	9
+GCL	49.50	2
+GCL	50.25	3
+GCL	51.01	6
+GCL	51.51	4
+GCL	52.01	1
+GCL	52.51	0
+GCL	53.02	1
+# ACGT content per cycle. Use `grep ^GCC | cut -f 2-` to extract this part. The columns are: cycle, and A,C,G,T counts [%]
+GCC	1	21.50	29.50	33.50	15.50
+GCC	2	30.00	16.00	11.00	43.00
+GCC	3	33.00	25.00	9.00	33.00
+GCC	4	17.00	29.00	13.00	41.00
+GCC	5	37.00	22.00	12.00	29.00
+GCC	6	36.00	26.00	17.00	21.00
+GCC	7	29.50	13.50	31.50	25.50
+GCC	8	50.50	14.50	19.50	15.50
+GCC	9	17.00	33.00	18.00	32.00
+GCC	10	37.00	14.00	21.00	28.00
+GCC	11	20.50	14.50	29.50	35.50
+GCC	12	30.00	24.00	22.00	24.00
+GCC	13	27.00	15.00	21.00	37.00
+GCC	14	24.00	22.00	26.00	28.00
+GCC	15	25.50	20.50	24.50	29.50
+GCC	16	31.00	15.00	20.00	34.00
+GCC	17	28.00	25.00	14.00	33.00
+GCC	18	30.50	28.50	19.50	21.50
+GCC	19	29.00	26.00	21.00	24.00
+GCC	20	22.50	23.50	17.50	36.50
+GCC	21	35.50	17.50	19.50	27.50
+GCC	22	37.50	28.50	15.50	18.50
+GCC	23	31.00	19.00	13.00	37.00
+GCC	24	37.00	12.00	22.00	29.00
+GCC	25	35.50	22.50	17.50	24.50
+GCC	26	33.50	18.50	15.50	32.50
+GCC	27	34.50	14.50	25.50	25.50
+GCC	28	31.00	14.00	24.00	31.00
+GCC	29	30.00	27.00	24.00	19.00
+GCC	30	31.00	20.00	14.00	35.00
+GCC	31	33.50	29.50	13.50	23.50
+GCC	32	42.50	20.50	19.50	17.50
+GCC	33	25.50	23.50	14.50	36.50
+GCC	34	39.50	16.50	20.50	23.50
+GCC	35	32.50	23.50	21.50	22.50
+GCC	36	42.00	25.00	16.00	17.00
+GCC	37	38.00	17.00	19.00	26.00
+GCC	38	24.00	26.00	25.00	25.00
+GCC	39	22.50	41.50	18.50	17.50
+GCC	40	32.00	16.00	21.00	31.00
+GCC	41	33.00	28.00	19.00	20.00
+GCC	42	30.50	25.50	19.50	24.50
+GCC	43	35.00	29.00	15.00	21.00
+GCC	44	20.00	27.00	22.00	31.00
+GCC	45	40.50	21.50	21.50	16.50
+GCC	46	26.50	20.50	22.50	30.50
+GCC	47	38.50	29.50	16.50	15.50
+GCC	48	27.50	24.50	17.50	30.50
+GCC	49	28.50	32.50	10.50	28.50
+GCC	50	46.50	20.50	9.50	23.50
+GCC	51	34.50	28.50	13.50	23.50
+GCC	52	41.50	23.50	20.50	14.50
+GCC	53	20.00	28.00	26.00	26.00
+GCC	54	31.50	18.50	24.50	25.50
+GCC	55	30.50	22.50	16.50	30.50
+GCC	56	33.50	22.50	13.50	30.50
+GCC	57	23.00	24.00	23.00	30.00
+GCC	58	25.00	37.00	19.00	19.00
+GCC	59	34.00	23.00	24.00	19.00
+GCC	60	29.00	28.00	17.00	26.00
+GCC	61	25.50	23.50	24.50	26.50
+GCC	62	31.50	22.50	16.50	29.50
+GCC	63	27.50	28.50	25.50	18.50
+GCC	64	33.50	21.50	25.50	19.50
+GCC	65	35.50	19.50	18.50	26.50
+GCC	66	34.00	25.00	15.00	26.00
+GCC	67	37.00	23.00	19.00	21.00
+GCC	68	36.50	29.50	13.50	20.50
+GCC	69	38.50	19.50	20.50	21.50
+GCC	70	38.50	16.50	18.50	26.50
+GCC	71	25.50	38.50	21.50	14.50
+GCC	72	29.00	29.00	25.00	17.00
+GCC	73	32.50	20.50	21.50	25.50
+GCC	74	28.50	32.50	12.50	26.50
+GCC	75	41.50	12.50	18.50	27.50
+GCC	76	24.50	29.50	23.50	22.50
+GCC	77	36.00	21.00	18.00	25.00
+GCC	78	27.00	34.00	22.00	17.00
+GCC	79	21.50	26.50	25.50	26.50
+GCC	80	34.00	19.00	28.00	19.00
+GCC	81	17.00	26.00	26.00	31.00
+GCC	82	31.00	30.00	23.00	16.00
+GCC	83	31.50	26.50	12.50	29.50
+GCC	84	19.00	41.00	21.00	19.00
+GCC	85	37.50	24.50	16.50	21.50
+GCC	86	15.00	48.00	15.00	22.00
+GCC	87	41.00	16.00	18.00	25.00
+GCC	88	23.50	27.50	27.50	21.50
+GCC	89	26.50	27.50	26.50	19.50
+GCC	90	18.50	23.50	24.50	33.50
+GCC	91	27.00	32.00	22.00	19.00
+GCC	92	23.50	17.50	27.50	31.50
+GCC	93	25.50	37.50	15.50	21.50
+GCC	94	27.00	17.00	24.00	32.00
+GCC	95	26.50	37.50	14.50	21.50
+GCC	96	29.50	25.50	16.50	28.50
+GCC	97	29.00	31.00	21.00	19.00
+GCC	98	18.00	33.00	22.00	27.00
+GCC	99	24.50	33.50	24.50	17.50
+GCC	100	24.50	16.50	24.50	34.50
+GCC	101	25.00	40.00	19.00	16.00
+GCC	102	17.50	17.50	32.50	32.50
+GCC	103	31.00	26.00	16.00	27.00
+GCC	104	26.50	29.50	20.50	23.50
+GCC	105	34.00	33.00	21.00	12.00
+GCC	106	23.00	31.00	26.00	20.00
+GCC	107	17.50	35.50	23.50	23.50
+GCC	108	24.50	30.50	23.50	21.50
+GCC	109	17.00	31.00	22.00	30.00
+GCC	110	16.00	35.00	24.00	25.00
+GCC	111	24.00	32.00	23.00	21.00
+GCC	112	37.00	28.00	16.00	19.00
+GCC	113	19.50	22.50	32.50	25.50
+GCC	114	17.00	31.00	35.00	17.00
+GCC	115	29.50	24.50	23.50	22.50
+GCC	116	22.00	30.00	34.00	14.00
+GCC	117	27.00	23.00	19.00	31.00
+GCC	118	25.50	14.50	34.50	25.50
+GCC	119	22.50	34.50	20.50	22.50
+GCC	120	17.50	24.50	26.50	31.50
+GCC	121	27.50	33.50	22.50	16.50
+GCC	122	17.00	23.00	25.00	35.00
+GCC	123	23.50	46.50	11.50	18.50
+GCC	124	9.00	32.00	34.00	25.00
+GCC	125	24.00	27.00	19.00	30.00
+GCC	126	26.00	17.00	28.00	29.00
+GCC	127	26.50	16.50	21.50	35.50
+GCC	128	18.00	34.00	31.00	17.00
+GCC	129	25.50	25.50	27.50	21.50
+GCC	130	25.00	20.00	22.00	33.00
+GCC	131	17.50	39.50	24.50	18.50
+GCC	132	21.00	28.00	23.00	28.00
+GCC	133	13.50	31.50	35.50	19.50
+GCC	134	24.50	19.50	30.50	25.50
+GCC	135	16.50	23.50	30.50	29.50
+GCC	136	28.00	32.00	15.00	25.00
+GCC	137	22.50	21.50	30.50	25.50
+GCC	138	14.50	34.50	24.50	26.50
+GCC	139	20.50	29.50	24.50	25.50
+GCC	140	17.00	23.00	30.00	30.00
+GCC	141	20.50	23.50	25.50	30.50
+GCC	142	18.00	29.00	38.00	15.00
+GCC	143	22.00	24.00	27.00	27.00
+GCC	144	21.50	30.50	26.50	21.50
+GCC	145	22.00	21.00	29.00	28.00
+GCC	146	25.00	16.00	39.00	20.00
+GCC	147	26.50	22.50	30.50	20.50
+GCC	148	12.50	28.50	36.50	22.50
+GCC	149	26.50	23.50	23.50	26.50
+GCC	150	14.00	29.00	24.00	33.00
+GCC	151	19.50	30.50	32.50	17.50
+GCC	152	18.50	17.50	29.50	34.50
+GCC	153	22.50	22.50	31.50	23.50
+GCC	154	22.00	21.00	29.00	28.00
+GCC	155	21.00	26.00	19.00	34.00
+GCC	156	14.50	23.50	35.50	26.50
+GCC	157	22.00	31.00	23.00	24.00
+GCC	158	22.50	29.50	24.50	23.50
+GCC	159	17.50	12.50	46.50	23.50
+GCC	160	24.50	26.50	26.50	22.50
+GCC	161	13.00	23.00	45.00	19.00
+GCC	162	31.50	16.50	22.50	29.50
+GCC	163	19.50	21.50	35.50	23.50
+GCC	164	29.00	18.00	21.00	32.00
+GCC	165	14.50	17.50	35.50	32.50
+GCC	166	17.50	37.50	28.50	16.50
+GCC	167	16.50	21.50	31.50	30.50
+GCC	168	14.00	25.00	30.00	31.00
+GCC	169	18.50	20.50	23.50	37.50
+GCC	170	19.00	23.00	28.00	30.00
+GCC	171	20.00	28.00	33.00	19.00
+GCC	172	19.00	20.00	32.00	29.00
+GCC	173	24.50	16.50	33.50	25.50
+GCC	174	17.50	23.50	33.50	25.50
+GCC	175	33.50	17.50	35.50	13.50
+GCC	176	16.50	32.50	28.50	22.50
+GCC	177	19.00	22.00	27.00	32.00
+GCC	178	16.00	26.00	30.00	28.00
+GCC	179	18.00	18.00	22.00	42.00
+GCC	180	21.00	22.00	34.00	23.00
+GCC	181	20.50	19.50	35.50	24.50
+GCC	182	32.50	18.50	22.50	26.50
+GCC	183	24.50	13.50	28.50	33.50
+GCC	184	15.00	29.00	30.00	26.00
+GCC	185	15.00	32.00	33.00	20.00
+GCC	186	22.50	23.50	34.50	19.50
+GCC	187	19.00	14.00	40.00	27.00
+GCC	188	27.50	21.50	27.50	23.50
+GCC	189	17.00	22.00	34.00	27.00
+GCC	190	23.00	30.00	23.00	24.00
+GCC	191	25.00	22.00	28.00	25.00
+GCC	192	34.50	24.50	13.50	27.50
+GCC	193	18.50	25.50	25.50	30.50
+GCC	194	18.50	33.50	24.50	23.50
+GCC	195	16.00	26.00	23.00	35.00
+GCC	196	21.50	25.50	24.50	28.50
+GCC	197	20.00	21.00	23.00	36.00
+GCC	198	17.00	21.00	37.00	25.00
+GCC	199	20.50	18.50	25.50	35.50
+GCC	200	21.00	29.00	21.00	29.00
+GCC	201	27.00	21.00	23.00	29.00
+GCC	202	21.50	24.50	19.50	34.50
+GCC	203	21.50	24.50	26.50	27.50
+GCC	204	27.00	29.00	24.00	20.00
+GCC	205	19.50	21.50	22.50	36.50
+GCC	206	26.50	24.50	21.50	27.50
+GCC	207	22.50	21.50	19.50	36.50
+GCC	208	14.00	35.00	29.00	22.00
+GCC	209	16.00	23.00	12.00	49.00
+GCC	210	18.50	19.50	40.50	21.50
+GCC	211	26.00	20.00	22.00	32.00
+GCC	212	21.00	31.00	18.00	30.00
+GCC	213	24.00	15.00	31.00	30.00
+GCC	214	17.50	24.50	25.50	32.50
+GCC	215	26.00	24.00	23.00	27.00
+GCC	216	21.50	17.50	25.50	35.50
+GCC	217	26.00	29.00	17.00	28.00
+GCC	218	20.00	27.00	21.00	32.00
+GCC	219	17.00	21.00	21.00	41.00
+GCC	220	25.50	23.50	23.50	27.50
+GCC	221	21.50	23.50	20.50	34.50
+GCC	222	21.50	21.50	18.50	38.50
+GCC	223	20.00	27.00	28.00	25.00
+GCC	224	22.50	22.50	24.50	30.50
+GCC	225	14.50	35.50	30.50	19.50
+GCC	226	20.00	23.00	26.00	31.00
+GCC	227	20.50	24.50	23.50	31.50
+GCC	228	33.00	19.00	26.00	22.00
+GCC	229	22.50	24.50	18.50	34.50
+GCC	230	21.00	32.00	16.00	31.00
+GCC	231	23.00	28.00	30.00	19.00
+GCC	232	23.50	21.50	12.50	42.50
+GCC	233	21.00	27.00	25.00	27.00
+GCC	234	16.50	27.50	22.50	33.50
+GCC	235	20.00	15.00	28.00	37.00
+GCC	236	28.00	23.00	21.00	28.00
+GCC	237	20.50	19.50	22.50	37.50
+GCC	238	21.50	29.50	24.50	24.50
+GCC	239	20.00	8.00	17.00	55.00
+GCC	240	28.00	24.00	16.00	32.00
+GCC	241	22.50	22.50	16.50	38.50
+GCC	242	29.00	25.00	13.00	33.00
+GCC	243	22.50	15.50	23.50	38.50
+GCC	244	20.50	23.50	16.50	39.50
+GCC	245	28.00	23.00	19.00	30.00
+GCC	246	21.00	29.00	25.00	25.00
+GCC	247	32.00	14.00	13.00	41.00
+GCC	248	18.00	18.00	25.00	39.00
+GCC	249	25.00	23.00	21.00	31.00
+GCC	250	27.50	22.50	17.50	32.50
+GCC	251	13.50	20.50	36.50	29.50
+# Insert sizes. Use `grep ^IS | cut -f 2-` to extract this part. The columns are: insert size, pairs total, inward oriented pairs, outward oriented pairs, other pairs
+# Read lengths. Use `grep ^RL | cut -f 2-` to extract this part. The columns are: read length, count
+RL	251	200
+# Indel distribution. Use `grep ^ID | cut -f 2-` to extract this part. The columns are: length, number of insertions, number of deletions
+ID	1	1	0
+ID	2	1	0
+ID	4	2	0
+ID	10	5	0
+ID	13	1	0
+ID	14	1	0
+ID	15	1	0
+ID	18	1	0
+ID	21	1	0
+ID	22	1	0
+ID	23	2	0
+ID	24	3	0
+ID	29	1	0
+ID	35	2	0
+ID	38	2	0
+# Indels per cycle. Use `grep ^IC | cut -f 2-` to extract this part. The columns are: cycle, number of insertions (fwd), .. (rev) , number of deletions (fwd), .. (rev)
+IC	2	1	0	0	0
+IC	4	2	0	0	0
+IC	5	3	0	0	0
+IC	210	2	0	0	0
+IC	219	1	0	0	0
+IC	220	1	0	0	0
+IC	224	2	0	0	0
+IC	225	2	0	0	0
+IC	226	1	0	0	0
+IC	228	1	0	0	0
+IC	230	1	0	0	0
+IC	233	1	0	0	0
+IC	234	1	0	0	0
+IC	235	1	0	0	0
+IC	236	1	0	0	0
+IC	239	1	0	0	0
+IC	240	1	0	0	0
+IC	241	1	0	0	0
+IC	247	1	0	0	0
+# Coverage distribution. Use `grep ^COV | cut -f 2-` to extract this part.
+COV	[1-1]	1	1
+COV	[7-7]	7	1
+COV	[18-18]	18	1
+COV	[24-24]	24	1
+COV	[25-25]	25	249
+# GC-depth. Use `grep ^GCD | cut -f 2-` to extract this part. The columns are: GC%, unique sequence percentiles, 10th, 25th, 50th, 75th and 90th depth percentile
+GCD	0.0	100.000	0.000	0.000	0.000	0.000	0.000
diff --git a/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2/gcc.tab b/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2/gcc.tab
new file mode 100644
index 00000000000..e36f8f21ca3
--- /dev/null
+++ b/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2/gcc.tab
@@ -0,0 +1,252 @@
+# ACGT content per cycle. The columns are: cycle, and A,C,G,T counts (percent)
+1	21.50	29.50	33.50	15.50
+2	30.00	16.00	11.00	43.00
+3	33.00	25.00	9.00	33.00
+4	17.00	29.00	13.00	41.00
+5	37.00	22.00	12.00	29.00
+6	36.00	26.00	17.00	21.00
+7	29.50	13.50	31.50	25.50
+8	50.50	14.50	19.50	15.50
+9	17.00	33.00	18.00	32.00
+10	37.00	14.00	21.00	28.00
+11	20.50	14.50	29.50	35.50
+12	30.00	24.00	22.00	24.00
+13	27.00	15.00	21.00	37.00
+14	24.00	22.00	26.00	28.00
+15	25.50	20.50	24.50	29.50
+16	31.00	15.00	20.00	34.00
+17	28.00	25.00	14.00	33.00
+18	30.50	28.50	19.50	21.50
+19	29.00	26.00	21.00	24.00
+20	22.50	23.50	17.50	36.50
+21	35.50	17.50	19.50	27.50
+22	37.50	28.50	15.50	18.50
+23	31.00	19.00	13.00	37.00
+24	37.00	12.00	22.00	29.00
+25	35.50	22.50	17.50	24.50
+26	33.50	18.50	15.50	32.50
+27	34.50	14.50	25.50	25.50
+28	31.00	14.00	24.00	31.00
+29	30.00	27.00	24.00	19.00
+30	31.00	20.00	14.00	35.00
+31	33.50	29.50	13.50	23.50
+32	42.50	20.50	19.50	17.50
+33	25.50	23.50	14.50	36.50
+34	39.50	16.50	20.50	23.50
+35	32.50	23.50	21.50	22.50
+36	42.00	25.00	16.00	17.00
+37	38.00	17.00	19.00	26.00
+38	24.00	26.00	25.00	25.00
+39	22.50	41.50	18.50	17.50
+40	32.00	16.00	21.00	31.00
+41	33.00	28.00	19.00	20.00
+42	30.50	25.50	19.50	24.50
+43	35.00	29.00	15.00	21.00
+44	20.00	27.00	22.00	31.00
+45	40.50	21.50	21.50	16.50
+46	26.50	20.50	22.50	30.50
+47	38.50	29.50	16.50	15.50
+48	27.50	24.50	17.50	30.50
+49	28.50	32.50	10.50	28.50
+50	46.50	20.50	9.50	23.50
+51	34.50	28.50	13.50	23.50
+52	41.50	23.50	20.50	14.50
+53	20.00	28.00	26.00	26.00
+54	31.50	18.50	24.50	25.50
+55	30.50	22.50	16.50	30.50
+56	33.50	22.50	13.50	30.50
+57	23.00	24.00	23.00	30.00
+58	25.00	37.00	19.00	19.00
+59	34.00	23.00	24.00	19.00
+60	29.00	28.00	17.00	26.00
+61	25.50	23.50	24.50	26.50
+62	31.50	22.50	16.50	29.50
+63	27.50	28.50	25.50	18.50
+64	33.50	21.50	25.50	19.50
+65	35.50	19.50	18.50	26.50
+66	34.00	25.00	15.00	26.00
+67	37.00	23.00	19.00	21.00
+68	36.50	29.50	13.50	20.50
+69	38.50	19.50	20.50	21.50
+70	38.50	16.50	18.50	26.50
+71	25.50	38.50	21.50	14.50
+72	29.00	29.00	25.00	17.00
+73	32.50	20.50	21.50	25.50
+74	28.50	32.50	12.50	26.50
+75	41.50	12.50	18.50	27.50
+76	24.50	29.50	23.50	22.50
+77	36.00	21.00	18.00	25.00
+78	27.00	34.00	22.00	17.00
+79	21.50	26.50	25.50	26.50
+80	34.00	19.00	28.00	19.00
+81	17.00	26.00	26.00	31.00
+82	31.00	30.00	23.00	16.00
+83	31.50	26.50	12.50	29.50
+84	19.00	41.00	21.00	19.00
+85	37.50	24.50	16.50	21.50
+86	15.00	48.00	15.00	22.00
+87	41.00	16.00	18.00	25.00
+88	23.50	27.50	27.50	21.50
+89	26.50	27.50	26.50	19.50
+90	18.50	23.50	24.50	33.50
+91	27.00	32.00	22.00	19.00
+92	23.50	17.50	27.50	31.50
+93	25.50	37.50	15.50	21.50
+94	27.00	17.00	24.00	32.00
+95	26.50	37.50	14.50	21.50
+96	29.50	25.50	16.50	28.50
+97	29.00	31.00	21.00	19.00
+98	18.00	33.00	22.00	27.00
+99	24.50	33.50	24.50	17.50
+100	24.50	16.50	24.50	34.50
+101	25.00	40.00	19.00	16.00
+102	17.50	17.50	32.50	32.50
+103	31.00	26.00	16.00	27.00
+104	26.50	29.50	20.50	23.50
+105	34.00	33.00	21.00	12.00
+106	23.00	31.00	26.00	20.00
+107	17.50	35.50	23.50	23.50
+108	24.50	30.50	23.50	21.50
+109	17.00	31.00	22.00	30.00
+110	16.00	35.00	24.00	25.00
+111	24.00	32.00	23.00	21.00
+112	37.00	28.00	16.00	19.00
+113	19.50	22.50	32.50	25.50
+114	17.00	31.00	35.00	17.00
+115	29.50	24.50	23.50	22.50
+116	22.00	30.00	34.00	14.00
+117	27.00	23.00	19.00	31.00
+118	25.50	14.50	34.50	25.50
+119	22.50	34.50	20.50	22.50
+120	17.50	24.50	26.50	31.50
+121	27.50	33.50	22.50	16.50
+122	17.00	23.00	25.00	35.00
+123	23.50	46.50	11.50	18.50
+124	9.00	32.00	34.00	25.00
+125	24.00	27.00	19.00	30.00
+126	26.00	17.00	28.00	29.00
+127	26.50	16.50	21.50	35.50
+128	18.00	34.00	31.00	17.00
+129	25.50	25.50	27.50	21.50
+130	25.00	20.00	22.00	33.00
+131	17.50	39.50	24.50	18.50
+132	21.00	28.00	23.00	28.00
+133	13.50	31.50	35.50	19.50
+134	24.50	19.50	30.50	25.50
+135	16.50	23.50	30.50	29.50
+136	28.00	32.00	15.00	25.00
+137	22.50	21.50	30.50	25.50
+138	14.50	34.50	24.50	26.50
+139	20.50	29.50	24.50	25.50
+140	17.00	23.00	30.00	30.00
+141	20.50	23.50	25.50	30.50
+142	18.00	29.00	38.00	15.00
+143	22.00	24.00	27.00	27.00
+144	21.50	30.50	26.50	21.50
+145	22.00	21.00	29.00	28.00
+146	25.00	16.00	39.00	20.00
+147	26.50	22.50	30.50	20.50
+148	12.50	28.50	36.50	22.50
+149	26.50	23.50	23.50	26.50
+150	14.00	29.00	24.00	33.00
+151	19.50	30.50	32.50	17.50
+152	18.50	17.50	29.50	34.50
+153	22.50	22.50	31.50	23.50
+154	22.00	21.00	29.00	28.00
+155	21.00	26.00	19.00	34.00
+156	14.50	23.50	35.50	26.50
+157	22.00	31.00	23.00	24.00
+158	22.50	29.50	24.50	23.50
+159	17.50	12.50	46.50	23.50
+160	24.50	26.50	26.50	22.50
+161	13.00	23.00	45.00	19.00
+162	31.50	16.50	22.50	29.50
+163	19.50	21.50	35.50	23.50
+164	29.00	18.00	21.00	32.00
+165	14.50	17.50	35.50	32.50
+166	17.50	37.50	28.50	16.50
+167	16.50	21.50	31.50	30.50
+168	14.00	25.00	30.00	31.00
+169	18.50	20.50	23.50	37.50
+170	19.00	23.00	28.00	30.00
+171	20.00	28.00	33.00	19.00
+172	19.00	20.00	32.00	29.00
+173	24.50	16.50	33.50	25.50
+174	17.50	23.50	33.50	25.50
+175	33.50	17.50	35.50	13.50
+176	16.50	32.50	28.50	22.50
+177	19.00	22.00	27.00	32.00
+178	16.00	26.00	30.00	28.00
+179	18.00	18.00	22.00	42.00
+180	21.00	22.00	34.00	23.00
+181	20.50	19.50	35.50	24.50
+182	32.50	18.50	22.50	26.50
+183	24.50	13.50	28.50	33.50
+184	15.00	29.00	30.00	26.00
+185	15.00	32.00	33.00	20.00
+186	22.50	23.50	34.50	19.50
+187	19.00	14.00	40.00	27.00
+188	27.50	21.50	27.50	23.50
+189	17.00	22.00	34.00	27.00
+190	23.00	30.00	23.00	24.00
+191	25.00	22.00	28.00	25.00
+192	34.50	24.50	13.50	27.50
+193	18.50	25.50	25.50	30.50
+194	18.50	33.50	24.50	23.50
+195	16.00	26.00	23.00	35.00
+196	21.50	25.50	24.50	28.50
+197	20.00	21.00	23.00	36.00
+198	17.00	21.00	37.00	25.00
+199	20.50	18.50	25.50	35.50
+200	21.00	29.00	21.00	29.00
+201	27.00	21.00	23.00	29.00
+202	21.50	24.50	19.50	34.50
+203	21.50	24.50	26.50	27.50
+204	27.00	29.00	24.00	20.00
+205	19.50	21.50	22.50	36.50
+206	26.50	24.50	21.50	27.50
+207	22.50	21.50	19.50	36.50
+208	14.00	35.00	29.00	22.00
+209	16.00	23.00	12.00	49.00
+210	18.50	19.50	40.50	21.50
+211	26.00	20.00	22.00	32.00
+212	21.00	31.00	18.00	30.00
+213	24.00	15.00	31.00	30.00
+214	17.50	24.50	25.50	32.50
+215	26.00	24.00	23.00	27.00
+216	21.50	17.50	25.50	35.50
+217	26.00	29.00	17.00	28.00
+218	20.00	27.00	21.00	32.00
+219	17.00	21.00	21.00	41.00
+220	25.50	23.50	23.50	27.50
+221	21.50	23.50	20.50	34.50
+222	21.50	21.50	18.50	38.50
+223	20.00	27.00	28.00	25.00
+224	22.50	22.50	24.50	30.50
+225	14.50	35.50	30.50	19.50
+226	20.00	23.00	26.00	31.00
+227	20.50	24.50	23.50	31.50
+228	33.00	19.00	26.00	22.00
+229	22.50	24.50	18.50	34.50
+230	21.00	32.00	16.00	31.00
+231	23.00	28.00	30.00	19.00
+232	23.50	21.50	12.50	42.50
+233	21.00	27.00	25.00	27.00
+234	16.50	27.50	22.50	33.50
+235	20.00	15.00	28.00	37.00
+236	28.00	23.00	21.00	28.00
+237	20.50	19.50	22.50	37.50
+238	21.50	29.50	24.50	24.50
+239	20.00	8.00	17.00	55.00
+240	28.00	24.00	16.00	32.00
+241	22.50	22.50	16.50	38.50
+242	29.00	25.00	13.00	33.00
+243	22.50	15.50	23.50	38.50
+244	20.50	23.50	16.50	39.50
+245	28.00	23.00	19.00	30.00
+246	21.00	29.00	25.00	25.00
+247	32.00	14.00	13.00	41.00
+248	18.00	18.00	25.00	39.00
+249	25.00	23.00	21.00	31.00
+250	27.50	22.50	17.50	32.50
+251	13.50	20.50	36.50	29.50
diff --git a/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2/mpc.tab b/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2/mpc.tab
new file mode 100644
index 00000000000..f0b6be70b2d
--- /dev/null
+++ b/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2/mpc.tab
@@ -0,0 +1,253 @@
+# Columns correspond to qualities, rows to cycles. First column is the cycle number, second is the number of N's and the rest is the number of mismatches
+1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	2	0	0	0	0	0	0
+2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	1	0	0	0	0	0	0
+3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	2	0	0	0	0	0	0
+4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	2	0	0	0	0	0	0
+5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+7	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+8	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+9	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+10	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+11	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+12	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+13	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+14	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+15	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+16	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+17	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+18	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+19	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+20	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+21	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+22	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+23	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+24	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
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+30	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
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+32	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+33	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+34	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
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+222	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+223	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+224	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+225	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+226	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+227	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+228	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+229	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+230	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+231	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+232	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0
+233	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
+234	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
+235	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+236	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
+237	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
+238	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+239	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
+240	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+241	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+242	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
+243	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+244	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+245	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+246	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0
+247	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	1	0	0	0
+248	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	3	2	0	0	0	0	0	0	0	0	2	1	0	0	0	0	0	0	0	2	0	0	0	3	0	0	0
+249	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	1	1	0	0	0	0	0	0	0	0	2	0	5	0	0	0	0	0	0	2	0	0	0	0	0	0	0
+250	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	2	2	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	1	2	0	0	0	2	0	0	0
+251	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	2	2	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	2	0	0	0	0	3	0	0	0
+252	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
diff --git a/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2/sn.tab b/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2/sn.tab
new file mode 100644
index 00000000000..605df46e7a1
--- /dev/null
+++ b/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2/sn.tab
@@ -0,0 +1,33 @@
+# Summary Numbers
+
+raw total sequences:	200
+filtered sequences:	0
+sequences:	200
+is sorted:	1
+1st fragments:	100
+last fragments:	100
+reads mapped:	25
+reads mapped and paired:	0	# paired-end technology bit set + both mates mapped
+reads unmapped:	175
+reads properly paired:	0	# proper-pair bit set
+reads paired:	200	# paired-end technology bit set
+reads duplicated:	0	# PCR or optical duplicate bit set
+reads MQ0:	6	# mapped and MQ=0
+reads QC failed:	0
+non-primary alignments:	0
+total length:	50200	# ignores clipping
+bases mapped:	6275	# ignores clipping
+bases mapped (cigar):	6275	# more accurate
+bases trimmed:	0
+bases duplicated:	0
+mismatches:	591	# from NM fields
+error rate:	9.418327e-02	# mismatches / bases mapped (cigar)
+average length:	251
+maximum length:	251
+average quality:	34.7
+insert size average:	0.0
+insert size standard deviation:	0.0
+inward oriented pairs:	0
+outward oriented pairs:	0
+pairs with other orientation:	0
+pairs on different chromosomes:	0
diff --git a/tool_collections/samtools/samtools_stats/test-data/samtools_stats_ref.fa b/tool_collections/samtools/samtools_stats/test-data/samtools_stats_ref.fa
new file mode 100644
index 00000000000..a4b24136e8e
--- /dev/null
+++ b/tool_collections/samtools/samtools_stats/test-data/samtools_stats_ref.fa
@@ -0,0 +1,239 @@
+>chrM
+GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGG
+GTATGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTC
+CTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTA
+ATTAATTAATGCTTGTAGGACATAATAATAACAATTGAATGTCTGCACAGCCACTTTCCACACAGACATC
+ATAACAAAAAATTTCCACCAAACCCCCCCTCCCCCGCTTCTGGCCACAGCACTTAAACACATCTCTGCCA
+AACCCCAAAAACAAAGAACCCTAACACCAGCCTAACCAGATTTCAAATTTTATCTTTTGGCGGTATGCAC
+TTTTAACAGTCACCCCCCAACTAACACATTATTTTCCCCTCCCACTCCCATACTACTAATCTCATCAATA
+CAACCCCCGCCCATCCTACCCAGCACACACACACCGCTGCTAACCCCATACCCCGAACCAACCAAACCCC
+AAAGACACCCCCCACAGTTTATGTAGCTTACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTC
+ACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAA
+GCATCCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGAACAAGCATCAAGCACGCAGC
+AATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAA
+ACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGA
+TTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACT
+CACCTGAGTTGTAAAAAACTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACAC
+ACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATC
+AACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATC
+CCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACCTCTTGCTCAGCCTATATA
+CCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTC
+AAGGTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCTTAT
+GAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAACTAAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGA
+AGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCA
+TTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTTGGACGAACCAGAGTGTA
+GCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCTGAGCTAAACCTA
+GCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCG
+ATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATA
+ATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCC
+AAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCA
+AAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGAT
+AGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTC
+CAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAG
+TAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAAC
+ATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG
+TAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCC
+AATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGA
+AAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGC
+ATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAA
+AGGTAGCATAATCACTTGTTCCTTAAATAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCT
+TACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGAAGACCCTA
+TGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT
+AAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCACCAG
+TCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACA
+GCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCG
+ATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGG
+AGTAATCCAGGTCGGTTTCTATCTACNTTCAAATTCCTCCCTGTACGAAAGGACAAGAGAAATAAGGCCT
+ACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATACCCACACCCACCCAAGA
+ACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAAT
+TCCTCTTCTTAACAACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCA
+TTCCTAATGCTTACCGAACGAAAAATTCTAGGCTATATACAACTACGCAAAGGCCCCAACGTTGTAGGCC
+CCTACGGGCTACTACAACCCTTCGCTGACGCCATAAAACTCTTCACCAAAGAGCCCCTAAAACCCGCCAC
+ATCTACCATCACCCTCTACATCACCGCCCCGACCTTAGCTCTCACCATCGCTCTTCTACTATGAACCCCC
+CTCCCCATACCCAACCCCCTGGTCAACCTCAACCTAGGCCTCCTATTTATTCTAGCCACCTCTAGCCTAG
+CCGTTTACTCAATCCTCTGATCAGGGTGAGCATCAAACTCAAACTACGCCCTGATCGGCGCACTGCGAGC
+AGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCTACTATCAACATTACTAATAAGTGGC
+TCCTTTAACCTCTCCACCCTTATCACAACACAAGAACACCTCTGATTACTCCTGCCATCATGACCCTTGG
+CCATAATATGATTTATCTCCACACTAGCAGAGACCAACCGAACCCCCTTCGACCTTGCCGAAGGGGAGTC
+CGAACTAGTCTCAGGCTTCAACATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTCATAGCCGAATAC
+ACAAACATTATTATAATAAACACCCTCACCACTACAATCTTCCTAGGAACAACATATGACGCACTCTCCC
+CTGAACTCTACACAACATATTTTGTCACCAAGACCCTACTTCTAACCTCCCTGTTCTTATGAATTCGAAC
+AGCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTA
+GCATTACTTATATGATATGTCTCCATACCCATTACAATCTCCAGCATTCCCCCTCAAACCTAAGAAATAT
+GTCTGATAAAAGAGTTACTTTGATAGAGTAAATAATAGGAGCTTAAACCCCCTTATTTCTAGGACTATGA
+GAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTC
+AGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGTTATACCCTTCCCGTACTAATTAATCCCCT
+GGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTT
+TTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTCCAGTTCTAACCAAAAAAATAAACCCTC
+GTTCCACAGAAGCTGCCATCAAGTATTTCCTCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTAT
+CCTCTTCAACAATATACTCTCCGGACAATGAACCATAACCAATACTACCAATCAATACTCATCATTAATA
+ATCATAATAGCTATAGCAATAAAACTAGGAATAGCCCCCTTTCACTTCTGAGTCCCAGAGGTTACCCAAG
+GCACCCCTCTGACATCCGGCCTGCTTCTTCTCACATGACAAAAACTAGCCCCCATCTCAATCATATACCA
+AATCTCTCCCTCACTAAACGTAAGCCTTCTCCTCACTCTCTCAATCTTATCCATCATAGCAGGCAGTTGA
+GGTGGATTAAACCAAACCCAGCTACGCAAAATCTTAGCATACTCCTCAATTACCCACATAGGATGAATAA
+TAGCAGTTCTACCGTACAACCCTAACATAACCATTCTTAATTTAACTATTTATATTATCCTAACTACTAC
+CGCATTCCTACTACTCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTA
+ACATGACTAACACCCTTAATTCCATCCACCCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTT
+TGCCCAAATGGGCCATTATCGAAGAATTCACAAAAAACAATAGCCTCATCATCCCCACCATCATAGCCAC
+CATCACCCTCCTTAACCTCTACTTCTACCTACGCCTAATCTACTCCACCTCAATCACACTACTCCCCATA
+TCTAACAACGTAAAAATAAAATGACAGTTTGAACATACAAAACCCACCCCATTCCTCCCCACACTCATCG
+CCCTTACCACGCTACTCCTACCTATCTCCCCTTTTATACTAATAATCTTATAGAAATTTAGGTTAAATAC
+AGACCAAGAGCCTTCAAAGCCCTCAGTAAGTTGCAATACTTAATTTCTGTAACAGCTAAGGACTGCAAAA
+CCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACCAATGGGA
+CTTAAACCCACAAACACTTAGTTAACAGCTAAGCACCCTAATCAACTGGCTTCAATCTACTTCTCCCGCC
+GCCGGGAAAAAAGGCGGGAGAAGCCCCGGCAGGTTTGAAGCTGCTTCTTCGAATTTGCAATTCAATATGA
+AAATCACCTCGGAGCTGGTAAAAAGAGGCCTAACCCCTGTCTTTAGATTTACAGTCCAATGCTTCACTCA
+GCCATTTTACCTCACCCCCACTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG
+AACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCC
+GAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCCATGCAT
+TTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAAT
+AATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGACTCTTACCTCCCTCTCTC
+CTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTAG
+CAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGTGTCTC
+CTCTATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCCTGCCATAACCCAATAC
+CAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAG
+CTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGG
+AGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACCA
+GGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTTGGATACATAGGTA
+TGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGG
+AATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAA
+GTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAG
+GATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGT
+ACTACACGACACGTACTACGTTGTAGCCCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATC
+ATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCC
+ATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAAT
+GCCCCGACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTC
+ATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCC
+TAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTCGA
+AGAACCCGTATACATAAAATCTAGACAAAAAAGGAAGGAATCGAACCCCCCAAAGCTGGTTTCAAGCCAA
+CCCCATGGCCTCCATGACTTTTTCAAAAAGGTATTAGAAAAACCATTTCATAACTTTGTCAAAGTTAAAT
+TATAGGCTAAATCCTATATATCTTAATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT
+ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCC
+TGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGA
+AACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTAC
+ATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACG
+AGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGA
+CCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACA
+TCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTC
+TAAACCAAACCACTTTCACCGCTACACGACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGC
+AAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTT
+ACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACCTTTTAAGTT
+AAAGATTAAGAGAACCAACACCTCTTTACAGTGAAATGCCCCAACTAAATACTACCGTATGGCCCACCAT
+AATTACCCCCATACTCCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACCACCTA
+CCTCCCTCACCAAAGCCCATAAAAATAAAAAATTATAACAAACCCTGAGAACCAAAATGAACGAAAATCT
+GTTCGCTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCT
+CTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATGACTAATCAAAC
+TAACCTCAAAACAAATGATAACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTT
+AATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACTA
+TCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCACAGTGATTATAGGCTTTCGCTCTAAGATTA
+AAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGA
+AACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCAC
+CTACTCATGCACCTAATTGGAAGCGCCACCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTATCA
+TCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGTTTTCAC
+ACTTCTAGTAAGCCTCTACCTGCACGACAACACATAATGACCCACCAATCACATGCCTATCATATAGTAA
+AACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCCTAGCCATGTGATT
+TCACTTCCACTCCATAACGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGG
+CGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATACG
+GGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCTGAGCCTTTTACCACTCCAG
+CCTAGCCCCTACCCCCCAATTAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAA
+GTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAA
+TAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCT
+CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTT
+GTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCC
+AACTAATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTGATACTGGCATTTTGT
+AGATGTGGTTTGACTATTTCTGTATGTCTCCATCTATTGATGAGGGTCTTACTCTTTTAGTATAAATAGT
+ACCGTTAACTTCCAATTAACTAGTTTTGACAACATTCAAAAAAGAGTAATAAACTTCGCCTTAATTTTAA
+TAATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAACGGCTACAT
+AGAAAAATCCACCCCTTACGAGTGCGGCTTCGACCCTATATCCCCCGCCCGCGTCCCTTTCTCCATAAAA
+TTCTTCTTAGTAGCTATTACCTTCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCTACCATGAG
+CCCTACAAACAACTAACCTGCCACTAATAGTTATGTCATCCCTCTTATTAATCATCATCCTAGCCCTAAG
+TCTGGCCTATGAGTGACTACAAAAAGGATTAGACTGAACCGAATTGGTATATAGTTTAAACAAAACGAAT
+GATTTCGACTCATTAAATTATGATAATCATATTTACCAAATGCCCCTCATTTACATAAATATTATACTAG
+CATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCTCATATCCTCCCTACTATGCCTAGA
+AGGAATAATACTATCGCTGTTCATTATAGCTACTCTCATAACCCTCAACACCCACTCCCTCTTAGCCAAT
+ATTGTGCCTATTGCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACTAGTCTCAA
+TCTCCAACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCA
+ACAATTATATTACTACCACTGACATGACTTTCCAAAAAACACATAATTTGAATCAACACAACCACCCACA
+GCCTAATTATTAGCATCATCCCTCTACTATTTTTTAACCAAATCAACAACAACCTATTTAGCTGTTCCCC
+AACCTTTTCCTCCGACCCCCTAACAACCCCCCTCCTAATACTAACTACCTGACTCCTACCCCTCACAATC
+ATGGCAAGCCAACGCCACTTATCCAGTGAACCACTATCACGAAAAAAACTCTACCTCTCTATACTAATCT
+CCCTACAAATCTCCTTAATTATAACATTCACAGCCACAGAACTAATCATATTTTATATCTTCTTCGAAAC
+CACACTTATCCCCACCTTGGCTATCATCACCCGATGAGGCAACCAGCCAGAACGCCTGAACGCAGGCACA
+TACTTCCTATTCTACACCCTAGTAGGCTCCCTTCCCCTACTCATCGCACTAATTTACACTCACAACACCC
+TAGGCTCACTAAACATTCTACTACTCACTCTCACTGCCCAAGAACTATCAAACTCCTGAGCCAACAACTT
+AATATGACTAGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACTTATGACTCCCT
+AAAGCCCATGTCGAAGCCCCCATCGCTGGGTCAATAGTACTTGCCGCAGTACTCTTAAAACTAGGCGGCT
+ATGGTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCCTACCCCTTCCTTGTACT
+ATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCA
+TACTCTTCAATCAGCCACATAGCCCTCGTAGTAACAGCCATTCTCATCCAAACCCCCTGAAGCTTCACCG
+GCGCAGTCATTCTCATAATCGCCCACGGGCTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACTA
+CGAACGCACTCACAGTCGCATCATAATCCTCTCTCAAGGACTTCAAACTCTACTCCCACTAATAGCTTTT
+TGATGACTTCTAGCAAGCCTCGCTAACCTCGCCTTACCCCCCACTATTAACCTACTGGGAGAACTCTCTG
+TGCTAGTAACCACGTTCTCCTGATCAAATATCACTCTCCTACTTACAGGACTCAACATACTAGTCACAGC
+CCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAAA
+CCCTCATTCACACGAGAAAACACCCTCATGTTCATACACCTATCCCCCATTCTCCTCCTATCCCTCAACC
+CCGACATCATTACCGGGTTTTCCTCTTGTAAATATAGTTTAACCAAAACATCAGATTGTGAATCTGACAA
+CAGAGGCTTACGACCCCTTATTTACCGAGAAAGCTCACAAGAACTGCTAACTCATGCCCCCATGTCTAAC
+AACATGGCTTTCTCAACTTTTAAAGGATAACAGCTATCCATTGGTCTTAGGCCCCAAAAATTTTGGTGCA
+ACTCCAAATAAAAGTAATAACCATGCACACTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCC
+ATCCTTACCACCCTCGTTAACCCTAACAAAAAAAACTCATACCCCCATTATGTAAAATCCATTGTCGCAT
+CCACCTTTATTATCAGTCTCTTCCCCACAACAATATTCATGTGCCTAGACCAAGAAGTTATTATCTCGAA
+CTGACACTGAGCCACAACCCAAACAACCCAGCTCTCCCTAAGCTTCAAACTAGACTACTTCTCCATAATA
+TTCATCCCTGTAGCATTGTTCGTTACATGGTCCATCATAGAATTCTCACTGTGATATATAAACTCAGACC
+CAAACATTAATCAGTTCTTCAAATATCTACTCATCTTCCTAATTACCATACTAATCTTAGTTACCGCTAA
+CAACCTATTCCAACTGTTCATCGGCTGAGAGGGCGTAGGAATTATATCCTTCTTGCTCATCAGTTGATGA
+TACGCCCGAGCAGATGCCAACACAGCAGCCATTCAAGCAATCCTATACAACCGTATCGGCGATATCGGTT
+TCATCCTCGCCTTAGCATGATTTATCCTACACTCCAACTCATGAGACCCACAACAAATAGCCCTTCTAAA
+CGCTAATCCAAGCCTCACCCCACTACTAGGCCTCCTCCTAGCAGCAGCAGGCAAATCAGCCCAATTAGGT
+CTCCACCCCTGACTCCCCTCAGCCATAGAAGGCCCCACCCCAGTCTCAGCCCTACTCCACTCAAGCACTA
+TAGTTGTAGCAGGAATCTTCTTACTCATCCGCTTCCACCCCCTAGCAGAAAATAGCCCACTAATCCAAAC
+TCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGCGCCCTTACACAAAATGACATC
+AAAAAAATCGTAGCCTTCTCCACTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCATCAACCAAC
+CACACCTAGCATTCCTGCACATCTGTACCCACGCCTTCTTCAAAGCCATACTATTTATGTGCTCCGGGTC
+CATCATCCACAACCTTAACAATGAACAAGATATTCGAAAAATAGGAGGACTACTCAAAACCATACCTCTC
+ACTTCAACCTCCCTCACCATTGGCAGCCTAGCATTAGCAGGAATACCTTTCCTCACAGGTTTCTACTCCA
+AAGACCACATCATCGAAACCGCAAACATATCATACACAAACGCCTGAGCCCTATCTATTACTCTCATCGC
+TACCTCCCTGACAAGCGCCTATAGCACTCGAATAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCC
+ACCCTTACTAACATTAACGAAAATAACCCCACCCTACTAAACCCCATTAAACGCCTGGCAGCCGGAAGCC
+TATTCGCAGGATTTCTCATTACTAACAACATTTCCCCCGCATCCCCCTTCCAAACAACAATCCCCCTCTA
+CCTAAAACTCACAGCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTACCTAACC
+AACAAACTTAAAATAAAATCCCCACTATGCACATTTTATTTCTCCAACATACTCGGATTCTACCCTAGCA
+TCACACACCGCACAATCCCCTATCTAGGCCTTCTTACGAGCCAAAACCTGCCCCTACTCCTCCTAGACCT
+AACCTGACTAGAAAAGCTATTACCTAAAACAATTTCACAGCACCAAATCTCCACCTCCATCATCACCTCA
+ACCCAAAAAGGCATAATTAAACTTTACTTCCTCTCTTTCTTCTTCCCACTCATCCTAACCCTACTCCTAA
+TCACATAACCTATTCCCCCGAGCAATCTCAATTACAATATATACACCAACAAACAATGTTCAACCAGTAA
+CTACTACTAATCAACGCCCATAATCATACAAAGCCCCCGCACCAATAGGATCCTCCCGAATCAACCCTGA
+CCCCTCTCCTTCATAAATTATTCAGCTTCCTACACTATTAAAGTTTACCACAACCACCACCCCATCATAC
+TCTTTCACCCACAGCACCAATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAACCC
+CTGACCCCCATGCCTCAGGATACTCCTCAATAGCCATCGCTGTAGTATATCCAAAGACAACCATCATTCC
+CCCTAAATAAATTAAAAAAACTATTAAACCCATATAACCTCCCCCAAAATTCAGAATAATAACACACCCG
+ACCACACCGCTAACAATCAATACTAAACCCCCATAAATAGGAGAAGGCTTAGAAGAAAACCCCACAAACC
+CCATTACTAAACCCACACTCAACAGAAACAAAGCATACATCATTATTCTCGCACGGACTACAACCACGAC
+CAATGATATGAAAAACCATCGTTGTATTTCAACTACAAGAACACCAATGACCCCAATACGCAAAACTAAC
+CCCCTAATAAAATTAATTAACCACTCATTCATCGACCTCCCCACCCCATCCAACATCTCCGCATGATGAA
+ACTTCGGCTCACTCCTTGGCGCCTGCCTGATCCTCCAAATCACCACAGGACTATTCCTAGCCATGCACTA
+CTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCACATCACTCGAGACGTAAATTATGGCTGAATC
+ATCCGCTACCTTCACGCCAATGGCGCCTCAATATTCTTTATCTGCCTCTTCCTACACATCGGGCGAGGCC
+TATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCATTATCCTCCTGCTTGCAACTATAGC
+AACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATCATTCTGAGGGGCCACAGTAATTACAAAC
+TTACTATCCGCCATCCCATACATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA
+GTCCCACCCTCACACGATTCTTTACCTTTCACTTCATCTTGCCCTTCATTATTGCAGCCCTAGCAACACT
+CCACCTCCTATTCTTGCACGAAACGGGATCAAACAACCCCCTAGGAATCACCTCCCATTCCGATAAAATC
+ACCTTCCACCCTTACTACACAATCAAAGACGCCCTCGGCTTACTTCTCTTCCTTCTCTCCTTAATGACAT
+TAACACTATTCTCACCAGACCTCCTAGGCGACCCAGACAATTATACCCTAGCCAACCCCTTAAACACCCC
+TCCCCACATCAAGCCCGAATGATATTTCCTATTCGCCTACACAATTCTCCGATCCGTCCCTAACAAACTA
+GGAGGCGTCCTTGCCCTATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCAAAC
+AACAAAGCATAATATTTCGCCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCAGACCTCCTCATTCT
+AACCTGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGTAGCATCCGTACTATAC
+TTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAATTGAAAACAAAATACTCAAATGGGCCTGTC
+CTTGTAGTATAAACTAATACACCAGTCTTGTAAACCGGAGATGAAAACCTTTTTCCAAGGACAAATCAGA
+GAAAAAGTCTTTAACTCCACCATTAGCACCCAAAGCTAAGATTCTAATTTAAACTATTCTCTGTTCTTTC
+ATGGGGAAGCAGATTTGGGTACCACCCAAGTATTGACTCACCCATCAACAACCGCTATGTATTTCGTACA
+TTACTGCCAGCCACCATGAATATTGTACGGTACCATAAATACTTGACCACCTGTAGTACATAAAAACCCA
+ATCCACATCAAAACCCCCTCCCCATGCTTACAAGCAAGTACAGCAATCAACCCTCAACTATCACACATCA
+ACTGCAACTCCAAAGCCACCCCTCACCCACTAGGATACCAACAAACCTACCCACCCTTAACAGTACATAG
+TACATAAAGCCATTTACCGTACATAGCACATTACAGTCAAATCCCTTCTCGTCCCCATGGATGACCCCCC
+TCAGATAGGGGTCCCTTGACCACCATCCTCCGTGAAATCAATATCCCGCACAAGAGTGCTACTCTCCTCG
+CTCCGGGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTC
+ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATG
+
diff --git a/tool_collections/samtools/samtools_stats/tool-data/fasta_indexes.loc.sample b/tool_collections/samtools/samtools_stats/tool-data/fasta_indexes.loc.sample
new file mode 100644
index 00000000000..fd8fb70ac28
--- /dev/null
+++ b/tool_collections/samtools/samtools_stats/tool-data/fasta_indexes.loc.sample
@@ -0,0 +1,29 @@
+#This is a sample file distributed with Galaxy that enables tools
+#to use a directory of Samtools indexed sequences data files.  You will need
+#to create these data files and then create a fasta_indexes.loc file
+#similar to this one (store it in this directory) that points to
+#the directories in which those files are stored. The fasta_indexes.loc
+#file has this format (white space characters are TAB characters):
+#
+# <unique_build_id>	<dbkey>	<display_name>	<file_base_path>
+#
+#So, for example, if you had hg19 Canonical indexed stored in
+#
+# /depot/data2/galaxy/hg19/sam/,
+#
+#then the fasta_indexes.loc entry would look like this:
+#
+#hg19canon	hg19	Human (Homo sapiens): hg19 Canonical	/depot/data2/galaxy/hg19/sam/hg19canon.fa
+#
+#and your /depot/data2/galaxy/hg19/sam/ directory
+#would contain hg19canon.fa and hg19canon.fa.fai files.
+#
+#Your fasta_indexes.loc file should include an entry per line for
+#each index set you have stored.  The file in the path does actually
+#exist, but it should never be directly used. Instead, the name serves
+#as a prefix for the index file.  For example:
+#
+#hg18canon	hg18	Human (Homo sapiens): hg18 Canonical	/depot/data2/galaxy/hg18/sam/hg18canon.fa
+#hg18full	hg18	Human (Homo sapiens): hg18 Full	/depot/data2/galaxy/hg18/sam/hg18full.fa
+#hg19canon	hg19	Human (Homo sapiens): hg19 Canonical	/depot/data2/galaxy/hg19/sam/hg19canon.fa
+#hg19full	hg19	Human (Homo sapiens): hg19 Full	/depot/data2/galaxy/hg19/sam/hg19full.fa
diff --git a/tool_collections/samtools/samtools_stats/tool_data_table_conf.xml.sample b/tool_collections/samtools/samtools_stats/tool_data_table_conf.xml.sample
new file mode 100644
index 00000000000..c34ade01890
--- /dev/null
+++ b/tool_collections/samtools/samtools_stats/tool_data_table_conf.xml.sample
@@ -0,0 +1,7 @@
+<!-- Use the file tool_data_table_conf.xml.oldlocstyle if you don't want to update your loc files as changed in revision 4550:535d276c92bc-->
+<tables>
+    <table name="fasta_indexes" comment_char="#">
+        <columns>value, dbkey, name, path</columns>
+        <file path="tool-data/fasta_indexes.loc" />
+    </table>
+</tables>
diff --git a/tool_collections/samtools/samtools_stats/tool_dependencies.xml b/tool_collections/samtools/samtools_stats/tool_dependencies.xml
new file mode 120000
index 00000000000..b11b378d63c
--- /dev/null
+++ b/tool_collections/samtools/samtools_stats/tool_dependencies.xml
@@ -0,0 +1 @@
+../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/tool_dependencies.xml b/tool_collections/samtools/tool_dependencies.xml
new file mode 100644
index 00000000000..8995249fe5f
--- /dev/null
+++ b/tool_collections/samtools/tool_dependencies.xml
@@ -0,0 +1,6 @@
+<?xml version="1.0"?>
+<tool_dependency>
+    <package name="samtools" version="1.2">
+        <repository name="package_samtools_1_2" owner="iuc" />
+    </package>
+</tool_dependency>
diff --git a/tool_collections/vcflib/LICENSE b/tool_collections/vcflib/LICENSE
new file mode 100644
index 00000000000..d176cde6c50
--- /dev/null
+++ b/tool_collections/vcflib/LICENSE
@@ -0,0 +1,21 @@
+The MIT License (MIT)
+
+Copyright (c) 2014 Anton Nekrutenko
+
+Permission is hereby granted, free of charge, to any person obtaining a copy
+of this software and associated documentation files (the "Software"), to deal
+in the Software without restriction, including without limitation the rights
+to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
+copies of the Software, and to permit persons to whom the Software is
+furnished to do so, subject to the following conditions:
+
+The above copyright notice and this permission notice shall be included in all
+copies or substantial portions of the Software.
+
+THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
+IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
+FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
+AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
+LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
+OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
+SOFTWARE.
\ No newline at end of file
diff --git a/tool_collections/vcflib/README.rst b/tool_collections/vcflib/README.rst
new file mode 100644
index 00000000000..d80c24d0e94
--- /dev/null
+++ b/tool_collections/vcflib/README.rst
@@ -0,0 +1,9 @@
+vcflib-tools
+============
+
+A collection of Galaxy wrappers for vcflib utilities. This is a
+development copy of the tools that exist in the Galaxy Tool Shed
+(http://usegalaxy.org/toolshed).
+
+Because vcflib can only be reliably compiled on Linux platforms at this
+time these wrappers make this assumption.
diff --git a/tool_collections/vcflib/macros.xml b/tool_collections/vcflib/macros.xml
new file mode 100644
index 00000000000..f5de643d57c
--- /dev/null
+++ b/tool_collections/vcflib/macros.xml
@@ -0,0 +1,29 @@
+<macros>
+    <xml name="requirements">
+        <requirements>
+            <requirement type="package" version="1.0.0_rc1">vcflib</requirement>
+            <yield/>
+        </requirements>
+    </xml>
+    <xml name="stdio">
+        <stdio>
+            <exit_code range="1:" level="fatal" />
+        </stdio>
+    </xml>
+   <token name="@WRAPPER_VERSION@">1.0.0_rc1</token>
+   	<xml name="citations">
+   	     <citations>
+             <citation type="bibtex">
+@misc{Garrison2015,
+  author = {Garrison, Erik},
+  year = {2015},
+  title = {vcflib},
+  publisher = {GitHub},
+  journal = {GitHub repository},
+  url = {https://github.com/ekg/vcflib},
+}
+             </citation>
+   	     </citations>
+   	</xml>
+    <token name="@IS_PART_OF_VCFLIB@">is a part of VCFlib toolkit developed by Erik Garrison (https://github.com/ekg/vcflib).</token>
+</macros>
diff --git a/tool_collections/vcflib/tool-data/fasta_indexes.loc.sample b/tool_collections/vcflib/tool-data/fasta_indexes.loc.sample
new file mode 100644
index 00000000000..fd8fb70ac28
--- /dev/null
+++ b/tool_collections/vcflib/tool-data/fasta_indexes.loc.sample
@@ -0,0 +1,29 @@
+#This is a sample file distributed with Galaxy that enables tools
+#to use a directory of Samtools indexed sequences data files.  You will need
+#to create these data files and then create a fasta_indexes.loc file
+#similar to this one (store it in this directory) that points to
+#the directories in which those files are stored. The fasta_indexes.loc
+#file has this format (white space characters are TAB characters):
+#
+# <unique_build_id>	<dbkey>	<display_name>	<file_base_path>
+#
+#So, for example, if you had hg19 Canonical indexed stored in
+#
+# /depot/data2/galaxy/hg19/sam/,
+#
+#then the fasta_indexes.loc entry would look like this:
+#
+#hg19canon	hg19	Human (Homo sapiens): hg19 Canonical	/depot/data2/galaxy/hg19/sam/hg19canon.fa
+#
+#and your /depot/data2/galaxy/hg19/sam/ directory
+#would contain hg19canon.fa and hg19canon.fa.fai files.
+#
+#Your fasta_indexes.loc file should include an entry per line for
+#each index set you have stored.  The file in the path does actually
+#exist, but it should never be directly used. Instead, the name serves
+#as a prefix for the index file.  For example:
+#
+#hg18canon	hg18	Human (Homo sapiens): hg18 Canonical	/depot/data2/galaxy/hg18/sam/hg18canon.fa
+#hg18full	hg18	Human (Homo sapiens): hg18 Full	/depot/data2/galaxy/hg18/sam/hg18full.fa
+#hg19canon	hg19	Human (Homo sapiens): hg19 Canonical	/depot/data2/galaxy/hg19/sam/hg19canon.fa
+#hg19full	hg19	Human (Homo sapiens): hg19 Full	/depot/data2/galaxy/hg19/sam/hg19full.fa
diff --git a/tool_collections/vcflib/tool-data/tool_data_table_conf.xml.sample b/tool_collections/vcflib/tool-data/tool_data_table_conf.xml.sample
new file mode 100644
index 00000000000..9111f18a24b
--- /dev/null
+++ b/tool_collections/vcflib/tool-data/tool_data_table_conf.xml.sample
@@ -0,0 +1,8 @@
+<!-- Use the file tool_data_table_conf.xml.oldlocstyle if you don't want to update your loc files as changed in revision 4550:535d276c92bc-->
+<tables>
+    <!-- Location of SAMTools indexes and other files -->
+    <table name="fasta_indexes" comment_char="#">
+        <columns>value, dbkey, name, path</columns>
+        <file path="tool-data/fasta_indexes.loc" />
+    </table>
+</tables>
diff --git a/tool_collections/vcflib/vcf2tsv/.shed.yml b/tool_collections/vcflib/vcf2tsv/.shed.yml
new file mode 100644
index 00000000000..5cc2e89eb48
--- /dev/null
+++ b/tool_collections/vcflib/vcf2tsv/.shed.yml
@@ -0,0 +1,14 @@
+categories:
+- Variant Analysis
+- Convert Formats
+description: Converts VCF files into tab-delimited format
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  Converts stdin or given VCF file to tab-delimited format, using
+  null string to replace empty values in the table. Specifying -g will output one
+  line per sample with genotype information. A part of the vcflib utilities developed
+  by Erik Garrison (https://github.com/ekg/vcflib).
+name: vcf2tsv
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcf2tsv
+type: unrestricted
diff --git a/tool_collections/vcflib/vcf2tsv/macros.xml b/tool_collections/vcflib/vcf2tsv/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcf2tsv/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcf2tsv/test-data/vcf2tsv-test1.tab b/tool_collections/vcflib/vcf2tsv/test-data/vcf2tsv-test1.tab
new file mode 100644
index 00000000000..30a213d7fe2
--- /dev/null
+++ b/tool_collections/vcflib/vcf2tsv/test-data/vcf2tsv-test1.tab
@@ -0,0 +1,28 @@
+CHROM	POS	ID	REF	ALT	QUAL	FILTER	AA	AC	AF	AN	DP	NS	DB	H2	SAMPLE	DP	GQ	GT	HQ
+19	111	.	A	C	9.6	.	.	.	.	.	.	.	0	0	NA00001	.	.	0|0	10,10
+19	111	.	A	C	9.6	.	.	.	.	.	.	.	0	0	NA00002	.	.	0|0	10,10
+19	111	.	A	C	9.6	.	.	.	.	.	.	.	0	0	NA00003	.	.	0/1	3,3
+19	112	.	A	G	10	.	.	.	.	.	.	.	0	0	NA00001	.	.	0|0	10,10
+19	112	.	A	G	10	.	.	.	.	.	.	.	0	0	NA00002	.	.	0|0	10,10
+19	112	.	A	G	10	.	.	.	.	.	.	.	0	0	NA00003	.	.	0/1	3,3
+20	14370	rs6054257	G	A	29	PASS	.	.	0.5	.	14	3	1	1	NA00001	1	48	0|0	51,51
+20	14370	rs6054257	G	A	29	PASS	.	.	0.5	.	14	3	1	1	NA00002	8	48	1|0	51,51
+20	14370	rs6054257	G	A	29	PASS	.	.	0.5	.	14	3	1	1	NA00003	5	43	1/1	.,.
+20	17330	.	T	A	3	q10	.	.	0.017	.	11	3	0	0	NA00001	3	49	0|0	58,50
+20	17330	.	T	A	3	q10	.	.	0.017	.	11	3	0	0	NA00002	5	3	0|1	65,3
+20	17330	.	T	A	3	q10	.	.	0.017	.	11	3	0	0	NA00003	3	41	0/0	.,.
+20	1110696	rs6040355	A	G,T	67	PASS	T	.	0.333,0.667	.	10	2	1	0	NA00001	6	21	1|2	23,27
+20	1110696	rs6040355	A	G,T	67	PASS	T	.	0.333,0.667	.	10	2	1	0	NA00002	0	2	2|1	18,2
+20	1110696	rs6040355	A	G,T	67	PASS	T	.	0.333,0.667	.	10	2	1	0	NA00003	4	35	2/2	.,.
+20	1230237	.	T	.	47	PASS	T	.	.	.	13	3	0	0	NA00001	.	54	0|0	56,60
+20	1230237	.	T	.	47	PASS	T	.	.	.	13	3	0	0	NA00002	4	48	0|0	51,51
+20	1230237	.	T	.	47	PASS	T	.	.	.	13	3	0	0	NA00003	2	61	0/0	.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	G	3,1	.	6	9	3	0	0	NA00001	4	.	0/1	.
+20	1234567	microsat1	G	GA,GAC	50	PASS	G	3,1	.	6	9	3	0	0	NA00002	2	17	0/2	.
+20	1234567	microsat1	G	GA,GAC	50	PASS	G	3,1	.	6	9	3	0	0	NA00003	3	40	1/1	.
+20	1235237	.	T	.	0	.	.	.	.	.	.	.	0	0	NA00001	.	.	0/0	.
+20	1235237	.	T	.	0	.	.	.	.	.	.	.	0	0	NA00002	.	.	0|0	.
+20	1235237	.	T	.	0	.	.	.	.	.	.	.	0	0	NA00003	.	.	./.	.
+X	10	rsTest	AC	A,ATG	10	PASS	.	.	.	.	.	.	0	0	NA00001	.	.	0	.
+X	10	rsTest	AC	A,ATG	10	PASS	.	.	.	.	.	.	0	0	NA00002	.	.	0/1	.
+X	10	rsTest	AC	A,ATG	10	PASS	.	.	.	.	.	.	0	0	NA00003	.	.	0|2	.
diff --git a/tool_collections/vcflib/vcf2tsv/test-data/vcf2tsv-test2.tab b/tool_collections/vcflib/vcf2tsv/test-data/vcf2tsv-test2.tab
new file mode 100644
index 00000000000..2169204f24d
--- /dev/null
+++ b/tool_collections/vcflib/vcf2tsv/test-data/vcf2tsv-test2.tab
@@ -0,0 +1,13 @@
+CHROM	POS	ID	REF	ALT	QUAL	FILTER	AA	AC	AF	AN	DP	NS	DB	H2
+19	111	.	A	C	9.6	.	.	.	.	.	.	.	0	0
+19	112	.	A	G	10	.	.	.	.	.	.	.	0	0
+20	14370	rs6054257	G	A	29	PASS	.	.	0.5	.	14	3	1	1
+20	17330	.	T	A	3	q10	.	.	0.017	.	11	3	0	0
+20	1110696	rs6040355	A	G	67	PASS	T	.	0.333	.	10	2	1	0
+20	1110696	rs6040355	A	T	67	PASS	T	.	0.667	.	10	2	1	0
+20	1230237	.	T	.	47	PASS	T	.	.	.	13	3	0	0
+20	1234567	microsat1	G	GA	50	PASS	G	3	.	6	9	3	0	0
+20	1234567	microsat1	G	GAC	50	PASS	G	1	.	6	9	3	0	0
+20	1235237	.	T	.	0	.	.	.	.	.	.	.	0	0
+X	10	rsTest	AC	A	10	PASS	.	.	.	.	.	.	0	0
+X	10	rsTest	AC	ATG	10	PASS	.	.	.	.	.	.	0	0
diff --git a/tool_collections/vcflib/vcf2tsv/test-data/vcflib.vcf b/tool_collections/vcflib/vcf2tsv/test-data/vcflib.vcf
new file mode 100644
index 00000000000..e8dd794d9bd
--- /dev/null
+++ b/tool_collections/vcflib/vcf2tsv/test-data/vcflib.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	NS=3;DP=14;AF=0.5;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	NS=3;DP=11;AF=0.017	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	NS=2;DP=10;AF=0.333,0.667;AA=T;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	NS=3;DP=13;AA=T	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	NS=3;DP=9;AA=G;AN=6;AC=3,1	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	.	.	.	GT	0/0	0|0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcf2tsv/vcf2tsv.xml b/tool_collections/vcflib/vcf2tsv/vcf2tsv.xml
new file mode 100644
index 00000000000..90aa4c5206e
--- /dev/null
+++ b/tool_collections/vcflib/vcf2tsv/vcf2tsv.xml
@@ -0,0 +1,45 @@
+<tool id="vcf2tsv" name="VCFtoTab-delimited:" version="@WRAPPER_VERSION@.0">
+  <description>Convert VCF data into TAB-delimited format</description>
+  <macros>
+      <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"/>
+  <expand macro="stdio" />
+  <command>vcf2tsv ${g_option} -n '${null_filler}' '${input}' > '${out_file1}'</command>
+  <inputs>
+    <param format="vcf" name="input" type="data" label="Select VCF dataset to convert"/>
+    <param name="g_option" type="boolean" checked="true" truevalue="-g" falsevalue="" label="Report data per sample" help="-g option"/>
+    <param name="null_filler" type="select" label="Fill empty fields with" help="-n option" >
+      <option value="">Nothing</option>
+      <option value=".">.</option>
+      <option value="*">*</option>
+      <option value="NULL">NULL</option>
+    </param>
+  </inputs>
+  <outputs>
+    <data format="tabular" name="out_file1" />
+  </outputs>
+  <tests>
+    <test>
+      <param name="g_option" value="true"/>
+      <param name="null_filler" value="."/>
+      <param name="input" value="vcflib.vcf"/>
+      <output name="out_file1" file="vcf2tsv-test1.tab"/>
+    </test>
+   <test>
+      <param name="g_option" value="false"/>
+      <param name="null_filler" value="."/>
+      <param name="input" value="vcflib.vcf"/>
+      <output name="out_file1" file="vcf2tsv-test2.tab"/>
+    </test>
+    </tests>
+  <help>
+Converts VCF dataset to tab-delimited format, using null string to replace empty values in the table.
+Specifying "**Report data per sample**" (-g) will output one line per sample with genotype information.
+
+----
+
+Vcf2Tsv @IS_PART_OF_VCFLIB@
+  </help>
+  <expand macro="citations" />
+</tool>
diff --git a/tool_collections/vcflib/vcfaddinfo/.shed.yml b/tool_collections/vcflib/vcfaddinfo/.shed.yml
new file mode 100644
index 00000000000..fd27f3c964c
--- /dev/null
+++ b/tool_collections/vcflib/vcfaddinfo/.shed.yml
@@ -0,0 +1,11 @@
+categories:
+- Variant Analysis
+description: Adds info fields from the second dataset which are not present in the
+  first dataset.
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  Adds info fields from the second dataset which are not present in the first dataset.
+name: vcfaddinfo
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfaddinfo
+type: unrestricted
diff --git a/tool_collections/vcflib/vcfaddinfo/macros.xml b/tool_collections/vcflib/vcfaddinfo/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcfaddinfo/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfaddinfo/test-data/vcfaddinfo-input1.vcf b/tool_collections/vcflib/vcfaddinfo/test-data/vcfaddinfo-input1.vcf
new file mode 100644
index 00000000000..f0aa4c60979
--- /dev/null
+++ b/tool_collections/vcflib/vcfaddinfo/test-data/vcfaddinfo-input1.vcf
@@ -0,0 +1,24 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+20	14370	rs6054257	G	A	29	PASS	NS=3;AF=0.5;DB;H2	GT:GQ:HQ	0|0:48:51,51	1|0:48:51,51	1/1:43:.,.
+20	17330	.	T	A	3	q10	NS=3;AF=0.017	GT:GQ:HQ	0|0:49:58,50	0|1:3:65,3	0/0:41:.,.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcfaddinfo/test-data/vcfaddinfo-test1.vcf b/tool_collections/vcflib/vcfaddinfo/test-data/vcfaddinfo-test1.vcf
new file mode 100644
index 00000000000..c782c8750e7
--- /dev/null
+++ b/tool_collections/vcflib/vcfaddinfo/test-data/vcfaddinfo-test1.vcf
@@ -0,0 +1,25 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+20	14370	rs6054257	G	A	29	PASS	AF=0.5;DP=14;NS=3;DB;H2	GT:GQ:HQ	0|0:48:51,51	1|0:48:51,51	1/1:43:.,.
+20	17330	.	T	A	3	q10	AF=0.017;DP=11;NS=3	GT:GQ:HQ	0|0:49:58,50	0|1:3:65,3	0/0:41:.,.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcfaddinfo/test-data/vcflib.vcf b/tool_collections/vcflib/vcfaddinfo/test-data/vcflib.vcf
new file mode 100644
index 00000000000..e8dd794d9bd
--- /dev/null
+++ b/tool_collections/vcflib/vcfaddinfo/test-data/vcflib.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	NS=3;DP=14;AF=0.5;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	NS=3;DP=11;AF=0.017	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	NS=2;DP=10;AF=0.333,0.667;AA=T;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	NS=3;DP=13;AA=T	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	NS=3;DP=9;AA=G;AN=6;AC=3,1	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	.	.	.	GT	0/0	0|0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcfaddinfo/vcfaddinfo.xml b/tool_collections/vcflib/vcfaddinfo/vcfaddinfo.xml
new file mode 100644
index 00000000000..8d4c52912a4
--- /dev/null
+++ b/tool_collections/vcflib/vcfaddinfo/vcfaddinfo.xml
@@ -0,0 +1,32 @@
+<tool id="vcfaddinfo" name="VCFaddinfo:" version="@WRAPPER_VERSION@.0">
+  <description>Adds info fields from the second dataset which are not present in the first dataset</description>
+  <macros>
+    <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"></expand>
+  <expand macro="stdio" />
+  <command>vcfaddinfo "${input1}" "${input2}" > "${out_file1}"</command>
+  <inputs>
+    <param format="vcf" name="input1" type="data" label="Add INFO fields to" help="First VCF dataset"/>
+    <param format="vcf" name="input2" type="data" label="using INFO fields from" help="Second VCF dataset"/>
+  </inputs>
+  <outputs>
+    <data format="vcf" name="out_file1" />
+  </outputs>
+ <tests>
+    <test>
+      <param name="input1" value="vcfaddinfo-input1.vcf"/>
+      <param name="input2" value="vcflib.vcf"/>
+      <output name="out_file1" file="vcfaddinfo-test1.vcf" />
+    </test>
+    </tests>
+<help>
+
+Adds info fields from the second dataset which are not present in the first dataset.
+
+-----
+
+Vcfaddinfo @IS_PART_OF_VCFLIB@
+</help>
+  <expand macro="citations" />
+</tool>
diff --git a/tool_collections/vcflib/vcfallelicprimitives/.shed.yml b/tool_collections/vcflib/vcfallelicprimitives/.shed.yml
new file mode 100644
index 00000000000..22105f331c2
--- /dev/null
+++ b/tool_collections/vcflib/vcfallelicprimitives/.shed.yml
@@ -0,0 +1,14 @@
+categories:
+- Variant Analysis
+description: Splits alleleic primitives (gaps or mismatches) into multiple VCF lines
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  If multiple alleleic primitives (gaps or mismatches) are specified
+  in a single VCF record, this tools splits the record into multiple lines, but drops
+  all INFO fields.  "Pure" MNPs are split into multiple SNPs unless the -m flag is
+  provided.  Genotypes are phased where complex alleles have been decomposed, provided
+  genotypes in the input.
+name: vcfallelicprimitives
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfallelicprimitives
+type: unrestricted
diff --git a/tool_collections/vcflib/vcfallelicprimitives/macros.xml b/tool_collections/vcflib/vcfallelicprimitives/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcfallelicprimitives/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfallelicprimitives/test-data/vcfallelicprimitives-test1.vcf b/tool_collections/vcflib/vcfallelicprimitives/test-data/vcfallelicprimitives-test1.vcf
new file mode 100644
index 00000000000..21a10473514
--- /dev/null
+++ b/tool_collections/vcflib/vcfallelicprimitives/test-data/vcfallelicprimitives-test1.vcf
@@ -0,0 +1,35 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+##INFO=<ID=TYPE,Number=A,Type=String,Description="The type of allele, either snp, mnp, ins, del, or complex.">
+##INFO=<ID=LEN,Number=A,Type=Integer,Description="allele length">
+##INFO=<ID=Split primitives,Number=0,Type=Flag,Description="The allele was parsed using vcfallelicprimitives.">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	AF=0.5;DP=14;NS=3;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	AF=0.017;DP=11;NS=3	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	.	A	G,T	67	PASS	AF=0.333,0.667;LEN=1,1;TYPE=snp,snp;Split primitives	GT	1|2	2|1	2|2
+20	1230237	.	T	.	47	PASS	AA=T;DP=13;NS=3	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	.	G	GA,GAC	50	PASS	AC=3,1;LEN=1,2;TYPE=ins,ins;Split primitives	GT	0|1	0|2	1|1
+20	1235237	.	T	.	0	.	.	GT	0/0	0|0	./.
+X	10	.	AC	ATC,A	10	PASS	LEN=1,1;TYPE=ins,del;Split primitives	GT	0	0|2	0|1
+X	11	.	C	G	10	PASS	LEN=1;TYPE=snp;Split primitives	GT	0	0|0	0|1
diff --git a/tool_collections/vcflib/vcfallelicprimitives/test-data/vcflib.vcf b/tool_collections/vcflib/vcfallelicprimitives/test-data/vcflib.vcf
new file mode 100644
index 00000000000..e8dd794d9bd
--- /dev/null
+++ b/tool_collections/vcflib/vcfallelicprimitives/test-data/vcflib.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	NS=3;DP=14;AF=0.5;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	NS=3;DP=11;AF=0.017	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	NS=2;DP=10;AF=0.333,0.667;AA=T;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	NS=3;DP=13;AA=T	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	NS=3;DP=9;AA=G;AN=6;AC=3,1	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	.	.	.	GT	0/0	0|0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcfallelicprimitives/vcfallelicprimitives.xml b/tool_collections/vcflib/vcfallelicprimitives/vcfallelicprimitives.xml
new file mode 100644
index 00000000000..c5498fbbc1a
--- /dev/null
+++ b/tool_collections/vcflib/vcfallelicprimitives/vcfallelicprimitives.xml
@@ -0,0 +1,62 @@
+<?xml version="1.0" encoding="utf-8"?>
+<tool id="vcfallelicprimitives" name="VcfAllelicPrimitives:" version="@WRAPPER_VERSION@.0">
+    <description>Split alleleic primitives (gaps or mismatches) into multiple VCF lines</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"></expand>
+    <expand macro="stdio" />
+    <command>
+        cat "${input}" | vcfallelicprimitives 
+            ${m_option} 
+            -t "${t_option}"
+            -L "${max_length}" 
+            $keep_info 
+            $keep_geno > "${out_file1}"
+    </command>
+    <inputs>
+        <param format="vcf" name="input" type="data" label="Select VCF dataset"/>
+        <param name="m_option" type="boolean" checked="false" truevalue="--use-mnps" falsevalue="" label="Retain MNPs as separate events" help="--use-mnps option"/>
+        <param name="t_option" type="text" value="Split primitives" label="Tag records which are split apart of a complex allele with this flag. " help="--tag-parsed option"/>
+        <param name="max_length" type="integer" value="200" label="Do not manipulate records in which either the ALT or REF is longer than (bp)" help="--max-length option"/>
+        <param name="keep_info" type="boolean" truevalue="--keep-info" falsevalue="" checked="False" 
+            label="Maintain site and allele-level annotations when decomposing" 
+            help="Note that in many cases, such as multisample VCFs, these won't be valid post-decomposition.  For biallelic loci in single-sample VCFs, they should be usable with caution. (--keep-info)"/>
+        <param name="keep_geno" type="boolean" truevalue="--keep-geno" falsevalue="" checked="False" 
+            label="Maintain genotype-level annotations when decomposing" 
+            help="Similar caution should be used for this as for --keep-info. (--keep-geno)"/>
+    </inputs>
+    <outputs>
+        <data format="vcf" name="out_file1" label="${tool.name} on ${on_string}" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="input" value="vcflib.vcf"/>
+            <output name="out_file1" file="vcfallelicprimitives-test1.vcf"/>
+        </test>
+    </tests>
+    <help>
+
+If multiple alleleic primitives (gaps or mismatches) are specified in a single VCF record, this tools splits the record into multiple lines, but drops all INFO fields.  
+"Pure" MNPs are split into multiple SNPs unless the -m flag is provided. 
+Genotypes are phased where complex alleles have been decomposed, provided genotypes in the input.
+
+The options are::
+
+    -m, --use-mnps          Retain MNPs as separate events (default: false).
+    -t, --tag-parsed FLAG   Tag records which are split apart of a complex allele with this flag.
+    -L, --max-length LEN    Do not manipulate records in which either the ALT or
+                            REF is longer than LEN (default: 200).
+    -k, --keep-info         Maintain site and allele-level annotations when decomposing.
+                            Note that in many cases, such as multisample VCFs, these won't
+                            be valid post-decomposition.  For biallelic loci in single-sample
+                            VCFs, they should be usable with caution.
+    -g, --keep-geno         Maintain genotype-level annotations when decomposing.  Similar
+                            caution should be used for this as for --keep-info.
+
+----
+
+Vcfallelicprimitives @IS_PART_OF_VCFLIB@
+    </help>
+    <expand macro="citations" />
+</tool>
diff --git a/tool_collections/vcflib/vcfannotate/.shed.yml b/tool_collections/vcflib/vcfannotate/.shed.yml
new file mode 100644
index 00000000000..a5339a1d867
--- /dev/null
+++ b/tool_collections/vcflib/vcfannotate/.shed.yml
@@ -0,0 +1,11 @@
+categories:
+- Variant Analysis
+description: Intersect VCF records with BED annotations
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  Intersects the records in the VCF file with targets provided in
+  a BED file. Intersections are done on the reference sequences in the VCF file.
+name: vcfannotate
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfannotate
+type: unrestricted
diff --git a/tool_collections/vcflib/vcfannotate/macros.xml b/tool_collections/vcflib/vcfannotate/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcfannotate/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfannotate/test-data/vcfannotate-test1.vcf b/tool_collections/vcflib/vcfannotate/test-data/vcfannotate-test1.vcf
new file mode 100644
index 00000000000..91655f5a2c1
--- /dev/null
+++ b/tool_collections/vcflib/vcfannotate/test-data/vcfannotate-test1.vcf
@@ -0,0 +1,32 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+##INFO=<ID=BED-features,Number=1,Type=String,Description="Annotation from /space/anton/galaxy-central/database/files/000/dataset_50.dat delimited by ':'">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	AF=0.5;DP=14;NS=3;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	AF=0.017;DP=11;NS=3	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	AA=T;AF=0.333,0.667;BED-features=uc010zpo.2:uc002wel.4:uc010zpp.2:uc002wen.4;DP=10;NS=2;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	AA=T;DP=13;NS=3	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	AA=G;AC=3,1;AN=6;DP=9;NS=3	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	0	.	.	GT	0/0	0|0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcfannotate/test-data/vcfannotate.bed b/tool_collections/vcflib/vcfannotate/test-data/vcfannotate.bed
new file mode 100644
index 00000000000..aa9c41604bf
--- /dev/null
+++ b/tool_collections/vcflib/vcfannotate/test-data/vcfannotate.bed
@@ -0,0 +1,46 @@
+20	123251	126392	uc002wcx.3	0	+	123269	126333	0	2	76,337,	0,2804,
+20	138110	139804	uc002wcy.2	0	+	138185	139665	0	2	124,390,	0,1304,
+20	168526	170264	uc002wcz.1	0	-	168526	170264	0	2	233,49,	0,1689,
+20	207898	210527	uc002wda.3	0	+	207929	210412	0	2	89,609,	0,2020,
+20	238376	241736	uc002wdb.3	0	+	238419	239947	0	2	101,2019,	0,1341,
+20	251503	271419	uc021vzl.1	0	-	251847	271244	0	11	405,119,87,102,158,100,159,119,118,49,192,	0,5105,5930,6181,6363,7467,8309,13100,18696,19396,19724,
+20	251503	271419	uc002wde.2	0	-	251847	271251	0	11	405,119,87,102,158,100,159,119,118,49,188,	0,5105,5930,6181,6363,7467,8309,13100,18696,19396,19728,
+20	251503	271419	uc010zpi.2	0	-	251847	270227	0	10	405,119,87,102,158,100,159,119,118,188,	0,5105,5930,6181,6363,7467,8309,13100,18696,19728,
+20	256608	271079	uc010zpj.1	0	-	257400	270981	0	7	912,102,158,100,159,119,180,	0,1076,1258,2362,3204,7995,14291,
+20	256608	271419	uc010zpk.2	0	-	257400	271232	0	7	912,102,158,100,159,119,188,	0,1076,1258,2362,3204,7995,14623,
+20	278203	280963	uc002wdf.3	0	+	278227	279442	0	1	2760,	0,
+20	306214	310872	uc002wdh.4	0	+	306568	307516	0	1	4658,	0,
+20	327369	335512	uc002wdi.4	0	+	330287	334279	0	4	417,115,195,1659,	0,2523,2912,6484,
+20	361307	378203	uc002wdm.3	0	+	368654	377334	0	4	506,291,293,1362,	0,7347,10623,15534,
+20	361940	378203	uc002wdn.3	0	+	363189	377334	0	5	204,194,291,293,1362,	0,1136,6714,9990,14901,
+20	388708	391408	uc002wdo.3	0	+	388708	388708	0	3	715,145,353,	0,1816,2347,
+20	388708	400504	uc010zpl.1	0	+	389401	400429	0	6	715,145,94,199,122,303,	0,1816,9461,9667,11282,11493,
+20	388708	409233	uc010zpm.1	0	+	388708	388708	0	9	715,145,94,199,122,174,112,180,99,	0,1816,9461,9667,11282,11493,14062,19248,20426,
+20	388708	411610	uc002wdp.4	0	+	389401	411074	0	12	715,145,94,199,122,174,161,112,180,99,144,617,	0,1816,9461,9667,11282,11493,12806,14062,19248,20426,20886,22285,
+20	388708	411610	uc002wdq.4	0	+	389382	411074	0	11	715,94,199,122,174,161,112,180,99,144,617,	0,9461,9667,11282,11493,12806,14062,19248,20426,20886,22285,
+20	388708	411610	uc010fzy.3	0	+	388708	388708	0	12	715,145,94,199,122,174,136,112,180,99,144,617,	0,1816,9461,9667,11282,11493,12806,14062,19248,20426,20886,22285,
+20	388708	411610	uc002wdr.4	0	+	398463	411074	0	10	715,94,199,122,174,112,180,99,144,617,	0,9461,9667,11282,11493,14062,19248,20426,20886,22285,
+20	416123	443187	uc002wds.3	0	-	419229	443049	0	8	3362,188,142,102,187,81,186,208,	0,3628,4768,6108,6377,9570,12409,26856,
+20	416123	443187	uc002wdt.3	0	-	416123	416123	0	10	491,159,292,188,142,102,187,81,186,208,	0,1960,3070,3628,4768,6108,6377,9570,12409,26856,
+20	416123	443187	uc002wdv.3	0	-	419229	422326	0	7	3362,188,142,456,81,186,208,	0,3628,4768,6108,9570,12409,26856,
+20	416125	417600	uc021vzm.1	0	-	416125	416125	0	1	1475,	0,
+20	463337	524482	uc002wdw.1	0	-	464604	489195	0	14	1383,87,149,101,102,111,84,60,51,102,112,210,117,167,	0,3682,4733,5984,7086,9560,13025,15027,16561,17139,22424,25757,45239,60978,
+20	463337	524482	uc002wdx.1	0	-	464604	489195	0	13	1383,87,149,101,102,111,84,60,51,102,112,210,167,	0,3682,4733,5984,7086,9560,13025,15027,16561,17139,22424,25757,60978,
+20	463337	524482	uc002wdy.1	0	-	464604	478382	0	12	1383,87,149,101,102,111,84,60,51,102,112,167,	0,3682,4733,5984,7086,9560,13025,15027,16561,17139,22424,60978,
+20	584636	590910	uc002wdz.3	0	-	585234	590881	0	2	673,554,	0,5720,
+20	627267	634014	uc002wea.4	0	-	629357	633829	0	2	2294,395,	0,6352,
+20	627267	656823	uc002web.4	0	-	629499	656245	0	3	2294,90,711,	0,7607,28845,
+20	642239	656823	uc002wec.3	0	-	644314	656245	0	2	2866,711,	0,13873,
+20	740723	749228	uc002wed.4	0	-	741669	746418	0	5	1159,124,506,618,288,	0,1621,3418,5128,8217,
+20	740724	749228	uc002wee.2	0	-	742293	746418	0	4	1744,506,618,288,	0,3417,5127,8216,
+20	814355	826922	uc002wef.1	0	+	825447	826335	0	2	239,1572,	0,10995,
+20	816710	826922	uc002weg.1	0	+	825447	826335	0	2	47,1572,	0,8640,
+20	825284	826922	uc002weh.1	0	+	825447	826335	0	1	1638,	0,
+20	853296	896960	uc002wei.3	0	-	853602	896857	0	9	467,131,167,102,116,248,122,156,412,	0,1630,5507,7093,8517,12424,15664,17559,43252,
+20	853296	896960	uc010zpn.2	0	-	853750	896839	0	8	467,167,102,116,248,122,156,412,	0,5507,7093,8517,12424,15664,17559,43252,
+20	939095	982907	uc002wej.3	0	-	940999	982807	0	5	2014,186,141,189,179,	0,5482,8721,9497,43633,
+20	939095	982907	uc002wek.3	0	-	940999	982807	0	4	2014,141,189,179,	0,8721,9497,43633,
+20	1093905	1147970	uc010zpo.2	0	+	1106275	1146898	0	7	147,153,83,186,54,159,1100,	0,12235,14163,21858,49868,51056,52965,
+20	1093905	1148426	uc002wel.4	0	+	1099416	1145724	0	8	147,150,153,83,186,54,159,2754,	0,5490,12235,14163,21858,49868,51056,51767,
+20	1099239	1147970	uc010zpp.2	0	+	1099416	1146898	0	6	306,153,83,54,159,1100,	0,6901,8829,44534,45722,47631,
+20	1099239	1148426	uc002wen.4	0	+	1099416	1145724	0	7	306,153,83,186,54,159,2754,	0,6901,8829,16524,44534,45722,46433,
diff --git a/tool_collections/vcflib/vcfannotate/test-data/vcflib.vcf b/tool_collections/vcflib/vcfannotate/test-data/vcflib.vcf
new file mode 100644
index 00000000000..e8dd794d9bd
--- /dev/null
+++ b/tool_collections/vcflib/vcfannotate/test-data/vcflib.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	NS=3;DP=14;AF=0.5;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	NS=3;DP=11;AF=0.017	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	NS=2;DP=10;AF=0.333,0.667;AA=T;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	NS=3;DP=13;AA=T	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	NS=3;DP=9;AA=G;AN=6;AC=3,1	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	.	.	.	GT	0/0	0|0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcfannotate/vcfannotate.xml b/tool_collections/vcflib/vcfannotate/vcfannotate.xml
new file mode 100644
index 00000000000..f1876f42e1a
--- /dev/null
+++ b/tool_collections/vcflib/vcfannotate/vcfannotate.xml
@@ -0,0 +1,51 @@
+<tool id="vcfannotate" name="VCFannotate:" version="@WRAPPER_VERSION@.0">
+  <description>Intersect VCF records with BED annotations</description>
+  <macros>
+    <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"/>
+  <expand macro="stdio" />
+  <command>vcfannotate --key "${key_option}" -b "${bed_data}" "${input}" > "${out_file1}"</command>
+  <inputs>
+    <param format="vcf" name="input" type="data" label="Select VCF dataset"/>
+    <param name="bed_data" type="data" format="bed" label="Select BED dataset to itersect with"/>
+    <param name="key_option" type="text" value="BED-features" label="Intersecting VCF records will be prefixed with this TAG within the INFO field" help="--key option"/>
+  </inputs>
+  <outputs>
+    <data format="vcf" name="out_file1" />
+  </outputs>
+  <tests>
+    <test>
+      <param name="bed_data" value="vcfannotate.bed" ftype="bed"/>
+      <param name="key_option" value="BED-features"/>
+      <param name="input" value="vcflib.vcf"/>
+      <output name="out_file1" file="vcfannotate-test1.vcf" lines_diff="2" />
+    </test>
+    </tests>
+  <help>
+
+Intersect the records in the VCF file with intervals (features) provided in a BED file. Intersections are done on the reference sequences in the VCF file. 
+
+-----
+
+.. class:: infomark
+
+
+**Example**:
+
+The following VCF line::
+
+ #CHROM POS     ID        REF ALT QUAL FILTER INFO                              FORMAT      NA00001        NA00002      NA00003
+ 20     1110696 rs6040355 A   G,T 67   PASS   NS=2;DP=10;AF=0.333,0.667;AA=T;DB GT:GQ:DP:HQ 1|2:21:6:23,27 2|1:2:0:18,2 2/2:35:4:.,.
+
+will appear as the follwing after intersectuion with BED records uc010zpo.2, uc002wel.4, uc010zpp.2, and uc002wen::
+
+ #CHROM POS     ID        REF ALT QUAL FILTER INFO                                                                                       FORMAT      NA00001        NA00002      NA00003                                                  
+ 20     1110696 rs6040355 A   G,T 67   PASS   AA=T;AF=0.333,0.667;BED-features=uc010zpo.2:uc002wel.4:uc010zpp.2:uc002wen.4;DP=10;NS=2;DB GT:GQ:DP:HQ 1|2:21:6:23,27 2|1:2:0:18,2 2/2:35:4:.,.
+
+----
+
+Vcfannotate @IS_PART_OF_VCFLIB@
+    </help>
+    <expand macro="citations" />
+</tool>
diff --git a/tool_collections/vcflib/vcfannotategenotypes/.shed.yml b/tool_collections/vcflib/vcfannotategenotypes/.shed.yml
new file mode 100644
index 00000000000..8853abfb1b5
--- /dev/null
+++ b/tool_collections/vcflib/vcfannotategenotypes/.shed.yml
@@ -0,0 +1,13 @@
+categories:
+- Variant Analysis
+description: 'Annotate genotypes in a VCF dataset using genotypes from another VCF dataset.'
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  Annotates genotypes in the first file with genotypes in the second
+  adding the genotype as another flag to each sample filed in the first file. Annotation-tag
+  is the name of the sample flag which is added to store the annotation.  also adds
+  a ''has\_variant'' flag for sites where the second file has a variant.
+name: vcfannotategenotypes
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfannotategenotypes
+type: unrestricted
diff --git a/tool_collections/vcflib/vcfannotategenotypes/macros.xml b/tool_collections/vcflib/vcfannotategenotypes/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcfannotategenotypes/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfannotategenotypes/test-data/vcfannotategenotypes-input2.vcf b/tool_collections/vcflib/vcfannotategenotypes/test-data/vcfannotategenotypes-input2.vcf
new file mode 100644
index 00000000000..ebfcec99b4b
--- /dev/null
+++ b/tool_collections/vcflib/vcfannotategenotypes/test-data/vcfannotategenotypes-input2.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	AF=0.5;DP=14;NS=3;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	AF=0.017;DP=11;NS=3	GT:GQ:DP:HQ	0|0:49:3:58,50	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	AA=T;AF=0.333,0.667;DP=10;NS=2;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	AA=T;DP=13;NS=3	GT:GQ:DP:HQ	0|0:54:.:56,60	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	AA=G;AC=3,1;AN=6;DP=9;NS=3	GT:GQ:DP	0/1:.:4	1/1:40:3
+20	1235237	.	T	.	0	.	.	GT	0/0	.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0|2
diff --git a/tool_collections/vcflib/vcfannotategenotypes/test-data/vcfannotategenotypes-test1.vcf b/tool_collections/vcflib/vcfannotategenotypes/test-data/vcfannotategenotypes-test1.vcf
new file mode 100644
index 00000000000..f867da394e7
--- /dev/null
+++ b/tool_collections/vcflib/vcfannotategenotypes/test-data/vcfannotategenotypes-test1.vcf
@@ -0,0 +1,33 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+##INFO=<ID=added-genotypes.has_variant,Number=0,Type=Flag,Description="True if added-genotypes has a called alternate among samples under comparison.">
+##FORMAT=<ID=added-genotypes,Number=1,Type=String,Description="Genotype from added-genotypes.">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	added-genotypes.has_variant	GT:HQ:added-genotypes	0|0:10,10:0/0	0|0:10,10:./.	0/1:3,3:0/1
+19	112	.	A	G	10	.	added-genotypes.has_variant	GT:HQ:added-genotypes	0|0:10,10:0/0	0|0:10,10:./.	0/1:3,3:0/1
+20	14370	rs6054257	G	A	29	PASS	AF=0.5;DP=14;NS=3;DB;H2;added-genotypes.has_variant	GT:GQ:DP:HQ:added-genotypes	0|0:48:1:51,51:0/0	1|0:48:8:51,51:./.	1/1:43:5:.,.:1/1
+20	17330	.	T	A	3	q10	AF=0.017;DP=11;NS=3;added-genotypes.has_variant	GT:GQ:DP:HQ:added-genotypes	0|0:49:3:58,50:0/0	0|1:3:5:65,3:./.	0/0:41:3:.,.:0/0
+20	1110696	rs6040355	A	G,T	67	PASS	AA=T;AF=0.333,0.667;DP=10;NS=2;DB;added-genotypes.has_variant	GT:GQ:DP:HQ:added-genotypes	1|2:21:6:23,27:1/2	2|1:2:0:18,2:./.	2/2:35:4:.,.:2/2
+20	1230237	.	T	.	47	PASS	AA=T;DP=13;NS=3;added-genotypes.has_variant	GT:GQ:DP:HQ:added-genotypes	0|0:54:.:56,60:0/0	0|0:48:4:51,51:./.	0/0:61:2:.,.:0/0
+20	1234567	microsat1	G	GA,GAC	50	PASS	AA=G;AC=3,1;AN=6;DP=9;NS=3;added-genotypes.has_variant	GT:GQ:DP:added-genotypes	0/1:.:4:0/1	0/2:17:2:./.	1/1:40:3:1/1
+20	1235237	.	T	.	0	.	added-genotypes.has_variant	GT:added-genotypes	0/0:0/0	0|0:./.	./.:.
+X	10	rsTest	AC	A,ATG	10	PASS	added-genotypes.has_variant	GT:added-genotypes	0:0	0/1:./.	0|2:0/2
diff --git a/tool_collections/vcflib/vcfannotategenotypes/test-data/vcflib.vcf b/tool_collections/vcflib/vcfannotategenotypes/test-data/vcflib.vcf
new file mode 100644
index 00000000000..e8dd794d9bd
--- /dev/null
+++ b/tool_collections/vcflib/vcfannotategenotypes/test-data/vcflib.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	NS=3;DP=14;AF=0.5;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	NS=3;DP=11;AF=0.017	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	NS=2;DP=10;AF=0.333,0.667;AA=T;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	NS=3;DP=13;AA=T	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	NS=3;DP=9;AA=G;AN=6;AC=3,1	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	.	.	.	GT	0/0	0|0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcfannotategenotypes/vcfannotategenotypes.xml b/tool_collections/vcflib/vcfannotategenotypes/vcfannotategenotypes.xml
new file mode 100644
index 00000000000..b9a363d5b89
--- /dev/null
+++ b/tool_collections/vcflib/vcfannotategenotypes/vcfannotategenotypes.xml
@@ -0,0 +1,34 @@
+<tool id="vcfannotategenotypes" name="VCFannotateGenotypes:" version="@WRAPPER_VERSION@.0">
+  <description>Annotate genotypes in a VCF dataset using genotypes from another VCF dataset</description>
+  <macros>
+    <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"/>
+  <expand macro="stdio" />
+  <command>vcfannotategenotypes "${tag_option}" "${input1}" "${input2}" > "${out_file1}"</command>
+  <inputs>
+    <param format="vcf" name="input1" type="data" label="Annotate genotypes in" help="First VCF dataset"/>
+    <param format="vcf" name="input2" type="data" label="using genotypes from" help="Second VCF dataset"/>
+    <param name="tag_option" type="text" value="added-genotypes" label="Mark genotypes added to the first dataset with this tag" help="Annotation tag"/>
+  </inputs>
+  <outputs>
+    <data format="vcf" name="out_file1" />
+  </outputs>
+ <tests>
+    <test>
+      <param name="tag_option" value="added-genotypes"/>
+      <param name="input1" value="vcflib.vcf"/>
+      <param name="input2" value="vcfannotategenotypes-input2.vcf"/>
+      <output name="out_file1" file="vcfannotategenotypes-test1.vcf" />
+    </test>
+    </tests>
+<help>
+
+Annotates genotypes in the **First** dataset with genotypes from the **Second** adding the genotype as another flag to each sample filled in the first file. **Annotation-tag** is the name of the sample flag which is added to store the annotation.  Also adds a 'has\_variant' flag for sites where the second file has a variant.
+
+-----
+
+Vcfannotate @IS_PART_OF_VCFLIB@
+</help>
+  <expand macro="citations" />
+</tool>
diff --git a/tool_collections/vcflib/vcfbedintersect/.shed.yml b/tool_collections/vcflib/vcfbedintersect/.shed.yml
new file mode 100644
index 00000000000..fe813c93942
--- /dev/null
+++ b/tool_collections/vcflib/vcfbedintersect/.shed.yml
@@ -0,0 +1,12 @@
+categories:
+- Variant Analysis
+description: Intersect VCF and BED datasets
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  Computes intersection between a VCF dataset and a set of genomic
+  intervals defined as either a BED dataset (http://genome.ucsc.edu/FAQ/FAQformat.html#format1)
+  or a manually typed interval (in the form of chr:start-end).
+name: vcfbedintersect
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfbedintersect
+type: unrestricted
diff --git a/tool_collections/vcflib/vcfbedintersect/macros.xml b/tool_collections/vcflib/vcfbedintersect/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcfbedintersect/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfbedintersect/test-data/vcfannotate.bed b/tool_collections/vcflib/vcfbedintersect/test-data/vcfannotate.bed
new file mode 100644
index 00000000000..aa9c41604bf
--- /dev/null
+++ b/tool_collections/vcflib/vcfbedintersect/test-data/vcfannotate.bed
@@ -0,0 +1,46 @@
+20	123251	126392	uc002wcx.3	0	+	123269	126333	0	2	76,337,	0,2804,
+20	138110	139804	uc002wcy.2	0	+	138185	139665	0	2	124,390,	0,1304,
+20	168526	170264	uc002wcz.1	0	-	168526	170264	0	2	233,49,	0,1689,
+20	207898	210527	uc002wda.3	0	+	207929	210412	0	2	89,609,	0,2020,
+20	238376	241736	uc002wdb.3	0	+	238419	239947	0	2	101,2019,	0,1341,
+20	251503	271419	uc021vzl.1	0	-	251847	271244	0	11	405,119,87,102,158,100,159,119,118,49,192,	0,5105,5930,6181,6363,7467,8309,13100,18696,19396,19724,
+20	251503	271419	uc002wde.2	0	-	251847	271251	0	11	405,119,87,102,158,100,159,119,118,49,188,	0,5105,5930,6181,6363,7467,8309,13100,18696,19396,19728,
+20	251503	271419	uc010zpi.2	0	-	251847	270227	0	10	405,119,87,102,158,100,159,119,118,188,	0,5105,5930,6181,6363,7467,8309,13100,18696,19728,
+20	256608	271079	uc010zpj.1	0	-	257400	270981	0	7	912,102,158,100,159,119,180,	0,1076,1258,2362,3204,7995,14291,
+20	256608	271419	uc010zpk.2	0	-	257400	271232	0	7	912,102,158,100,159,119,188,	0,1076,1258,2362,3204,7995,14623,
+20	278203	280963	uc002wdf.3	0	+	278227	279442	0	1	2760,	0,
+20	306214	310872	uc002wdh.4	0	+	306568	307516	0	1	4658,	0,
+20	327369	335512	uc002wdi.4	0	+	330287	334279	0	4	417,115,195,1659,	0,2523,2912,6484,
+20	361307	378203	uc002wdm.3	0	+	368654	377334	0	4	506,291,293,1362,	0,7347,10623,15534,
+20	361940	378203	uc002wdn.3	0	+	363189	377334	0	5	204,194,291,293,1362,	0,1136,6714,9990,14901,
+20	388708	391408	uc002wdo.3	0	+	388708	388708	0	3	715,145,353,	0,1816,2347,
+20	388708	400504	uc010zpl.1	0	+	389401	400429	0	6	715,145,94,199,122,303,	0,1816,9461,9667,11282,11493,
+20	388708	409233	uc010zpm.1	0	+	388708	388708	0	9	715,145,94,199,122,174,112,180,99,	0,1816,9461,9667,11282,11493,14062,19248,20426,
+20	388708	411610	uc002wdp.4	0	+	389401	411074	0	12	715,145,94,199,122,174,161,112,180,99,144,617,	0,1816,9461,9667,11282,11493,12806,14062,19248,20426,20886,22285,
+20	388708	411610	uc002wdq.4	0	+	389382	411074	0	11	715,94,199,122,174,161,112,180,99,144,617,	0,9461,9667,11282,11493,12806,14062,19248,20426,20886,22285,
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+20	388708	411610	uc002wdr.4	0	+	398463	411074	0	10	715,94,199,122,174,112,180,99,144,617,	0,9461,9667,11282,11493,14062,19248,20426,20886,22285,
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+20	416123	443187	uc002wdt.3	0	-	416123	416123	0	10	491,159,292,188,142,102,187,81,186,208,	0,1960,3070,3628,4768,6108,6377,9570,12409,26856,
+20	416123	443187	uc002wdv.3	0	-	419229	422326	0	7	3362,188,142,456,81,186,208,	0,3628,4768,6108,9570,12409,26856,
+20	416125	417600	uc021vzm.1	0	-	416125	416125	0	1	1475,	0,
+20	463337	524482	uc002wdw.1	0	-	464604	489195	0	14	1383,87,149,101,102,111,84,60,51,102,112,210,117,167,	0,3682,4733,5984,7086,9560,13025,15027,16561,17139,22424,25757,45239,60978,
+20	463337	524482	uc002wdx.1	0	-	464604	489195	0	13	1383,87,149,101,102,111,84,60,51,102,112,210,167,	0,3682,4733,5984,7086,9560,13025,15027,16561,17139,22424,25757,60978,
+20	463337	524482	uc002wdy.1	0	-	464604	478382	0	12	1383,87,149,101,102,111,84,60,51,102,112,167,	0,3682,4733,5984,7086,9560,13025,15027,16561,17139,22424,60978,
+20	584636	590910	uc002wdz.3	0	-	585234	590881	0	2	673,554,	0,5720,
+20	627267	634014	uc002wea.4	0	-	629357	633829	0	2	2294,395,	0,6352,
+20	627267	656823	uc002web.4	0	-	629499	656245	0	3	2294,90,711,	0,7607,28845,
+20	642239	656823	uc002wec.3	0	-	644314	656245	0	2	2866,711,	0,13873,
+20	740723	749228	uc002wed.4	0	-	741669	746418	0	5	1159,124,506,618,288,	0,1621,3418,5128,8217,
+20	740724	749228	uc002wee.2	0	-	742293	746418	0	4	1744,506,618,288,	0,3417,5127,8216,
+20	814355	826922	uc002wef.1	0	+	825447	826335	0	2	239,1572,	0,10995,
+20	816710	826922	uc002weg.1	0	+	825447	826335	0	2	47,1572,	0,8640,
+20	825284	826922	uc002weh.1	0	+	825447	826335	0	1	1638,	0,
+20	853296	896960	uc002wei.3	0	-	853602	896857	0	9	467,131,167,102,116,248,122,156,412,	0,1630,5507,7093,8517,12424,15664,17559,43252,
+20	853296	896960	uc010zpn.2	0	-	853750	896839	0	8	467,167,102,116,248,122,156,412,	0,5507,7093,8517,12424,15664,17559,43252,
+20	939095	982907	uc002wej.3	0	-	940999	982807	0	5	2014,186,141,189,179,	0,5482,8721,9497,43633,
+20	939095	982907	uc002wek.3	0	-	940999	982807	0	4	2014,141,189,179,	0,8721,9497,43633,
+20	1093905	1147970	uc010zpo.2	0	+	1106275	1146898	0	7	147,153,83,186,54,159,1100,	0,12235,14163,21858,49868,51056,52965,
+20	1093905	1148426	uc002wel.4	0	+	1099416	1145724	0	8	147,150,153,83,186,54,159,2754,	0,5490,12235,14163,21858,49868,51056,51767,
+20	1099239	1147970	uc010zpp.2	0	+	1099416	1146898	0	6	306,153,83,54,159,1100,	0,6901,8829,44534,45722,47631,
+20	1099239	1148426	uc002wen.4	0	+	1099416	1145724	0	7	306,153,83,186,54,159,2754,	0,6901,8829,16524,44534,45722,46433,
diff --git a/tool_collections/vcflib/vcfbedintersect/test-data/vcfbedintersect-test1.vcf b/tool_collections/vcflib/vcfbedintersect/test-data/vcfbedintersect-test1.vcf
new file mode 100644
index 00000000000..78d89f7d1be
--- /dev/null
+++ b/tool_collections/vcflib/vcfbedintersect/test-data/vcfbedintersect-test1.vcf
@@ -0,0 +1,23 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+20	1110696	rs6040355	A	G,T	67	PASS	NS=2;DP=10;AF=0.333,0.667;AA=T;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
diff --git a/tool_collections/vcflib/vcfbedintersect/test-data/vcfbedintersect-test2.vcf b/tool_collections/vcflib/vcfbedintersect/test-data/vcfbedintersect-test2.vcf
new file mode 100644
index 00000000000..06c9cd65726
--- /dev/null
+++ b/tool_collections/vcflib/vcfbedintersect/test-data/vcfbedintersect-test2.vcf
@@ -0,0 +1,24 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+20	14370	rs6054257	G	A	29	PASS	NS=3;DP=14;AF=0.5;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	NS=3;DP=11;AF=0.017	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
diff --git a/tool_collections/vcflib/vcfbedintersect/test-data/vcflib.vcf b/tool_collections/vcflib/vcfbedintersect/test-data/vcflib.vcf
new file mode 100644
index 00000000000..e8dd794d9bd
--- /dev/null
+++ b/tool_collections/vcflib/vcfbedintersect/test-data/vcflib.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	NS=3;DP=14;AF=0.5;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	NS=3;DP=11;AF=0.017	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	NS=2;DP=10;AF=0.333,0.667;AA=T;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	NS=3;DP=13;AA=T	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	NS=3;DP=9;AA=G;AN=6;AC=3,1	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	.	.	.	GT	0/0	0|0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcfbedintersect/vcfbedintersect.xml b/tool_collections/vcflib/vcfbedintersect/vcfbedintersect.xml
new file mode 100644
index 00000000000..0530de37eb9
--- /dev/null
+++ b/tool_collections/vcflib/vcfbedintersect/vcfbedintersect.xml
@@ -0,0 +1,59 @@
+<tool id="vcfbedintersect" name="VCF-BEDintersect:" version="@WRAPPER_VERSION@.0">
+    <description>Intersect VCF and BED datasets</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"/>
+    <expand macro="stdio" />
+    <command>
+        #if str($bed_vs_interval.bed_vs_interval_selector) == "bed":
+            vcfintersect -b "${bed_vs_interval.bed_input}" ${invert} "${vcf_input}" > "${out_file1}"
+        #else:
+            vcfintersect -R "${bed_vs_interval.int_input}" ${invert} "${vcf_input}" > "${out_file1}"
+        #end if
+    </command>
+    <inputs>
+        <!-- selecting refernce source -->
+        <param name="vcf_input" type="data" format="vcf" label="Select VCF dataset" />
+        <conditional name="bed_vs_interval">
+            <param name="bed_vs_interval_selector" type="select" label="BED dataset or an interval to intersect with">
+                <option value="bed">BED</option>
+                <option value="interval">Interval</option>
+            </param>
+            <when value="bed">
+                <param name="bed_input" type="data" format="bed" label="Select BED dataset" />
+            </when>
+            <when value="interval">
+                <param name="int_input" type="text" value="chr20:1-30" label="Enter interval string" help="use chr:start-end format" />
+            </when>
+        </conditional>
+        <param name="invert" type="boolean" truevalue="-v" falsevalue="" label="Invert selection?" help="-v, --invert. Print entries that DO NOT intersect." />
+    </inputs>
+    <outputs>
+        <data format="vcf" name="out_file1" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="bed_vs_interval_selector" value="bed" />
+            <param name="bed_input" value="vcfannotate.bed" ftype="bed" />
+            <param name="invert" value="False" />
+            <param name="vcf_input" value="vcflib.vcf"/>
+            <output name="out_file1" file="vcfbedintersect-test1.vcf"/>
+        </test>
+        <test>
+            <param name="bed_vs_interval_selector" value="interval" />
+            <param name="int_input" value="20:1-30000" />
+            <param name="invert" value="False" />
+            <param name="vcf_input" value="vcflib.vcf"/>
+            <output name="out_file1" file="vcfbedintersect-test2.vcf"/>
+        </test>
+    </tests>
+    <help>
+Computes intersection between a VCF dataset and a set of genomic intervals defined as either a BED dataset (http://genome.ucsc.edu/FAQ/FAQformat.html#format1) or a manually typed interval (in the form of chr:start-end).
+
+----
+
+VCFBEDintersect is based on vcfintersect utility of VCFlib toolkit developed by Erik Garrison (https://github.com/ekg/vcflib).
+    </help>
+    <expand macro="citations" />
+</tool>
diff --git a/tool_collections/vcflib/vcfbreakcreatemulti/.shed.yml b/tool_collections/vcflib/vcfbreakcreatemulti/.shed.yml
new file mode 100644
index 00000000000..4f46813a267
--- /dev/null
+++ b/tool_collections/vcflib/vcfbreakcreatemulti/.shed.yml
@@ -0,0 +1,17 @@
+categories:
+- Variant Analysis
+description: Break multiple alleles into multiple records, or combine overallpoing
+  alleles into a single record
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  This tool breaks or creates multiallelic VCF records based on user
+  selection.
+
+  - Breaking = If multiple alleles are specified in a single record, break the record
+  into multiple lines, preserving allele-specific INFO fields.
+  - Creation = If overlapping alleles are represented across multiple records, merge
+  them into a single record.
+name: vcfbreakcreatemulti
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfbreakcreatemulti
+type: unrestricted
diff --git a/tool_collections/vcflib/vcfbreakcreatemulti/macros.xml b/tool_collections/vcflib/vcfbreakcreatemulti/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcfbreakcreatemulti/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfbreakcreatemulti/test-data/vcfbreakcreatemulti-test1.vcf b/tool_collections/vcflib/vcfbreakcreatemulti/test-data/vcfbreakcreatemulti-test1.vcf
new file mode 100644
index 00000000000..9c4104947be
--- /dev/null
+++ b/tool_collections/vcflib/vcfbreakcreatemulti/test-data/vcfbreakcreatemulti-test1.vcf
@@ -0,0 +1,34 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	AF=0.5;DP=14;NS=3;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	AF=0.017;DP=11;NS=3	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G	67	PASS	AA=T;AF=0.333,0.667;DP=10;NS=2;DB	GT:GQ:DP:HQ	1|.:21:6:23,27	.|1:2:0:18,2	./.:35:4:.,.
+20	1110696	rs6040355	A	T	67	PASS	AA=T;AF=0.333,0.667;DP=10;NS=2;DB	GT:GQ:DP:HQ	.|1:21:6:23,27	1|.:2:0:18,2	1/1:35:4:.,.
+20	1230237	.	T	.	47	PASS	AA=T;DP=13;NS=3	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA	50	PASS	AA=G;AC=3,1;AN=6;DP=9;NS=3	GT:GQ:DP	0/1:.:4	./0:17:2	1/1:40:3
+20	1234567	microsat1	G	GAC	50	PASS	AA=G;AC=3,1;AN=6;DP=9;NS=3	GT:GQ:DP	./0:.:4	0/1:17:2	./.:40:3
+20	1235237	.	T	.	0	.	.	GT	0/0	0|0	./.
+X	10	rsTest	AC	A	10	PASS	.	GT	0	0/1	0|.
+X	10	rsTest	AC	ATG	10	PASS	.	GT	0	./0	0|1
diff --git a/tool_collections/vcflib/vcfbreakcreatemulti/test-data/vcfbreakcreatemulti-test2-input.vcf b/tool_collections/vcflib/vcfbreakcreatemulti/test-data/vcfbreakcreatemulti-test2-input.vcf
new file mode 100644
index 00000000000..acc549c6e0d
--- /dev/null
+++ b/tool_collections/vcflib/vcfbreakcreatemulti/test-data/vcfbreakcreatemulti-test2-input.vcf
@@ -0,0 +1,34 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	AF=0.5;DP=14;NS=3;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	AF=0.017;DP=11;NS=3	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G	67	PASS	AA=T;AF=0.333,0.667;DP=10;NS=2;DB	GT:GQ:DP:HQ	./1:21:6:23,27	./1:2:0:18,2	./.:35:4:.,.
+20	1110696	rs6040355	A	T	67	PASS	AA=T;AF=0.333,0.667;DP=10;NS=2;DB	GT:GQ:DP:HQ	./1:21:6:23,27	./1:2:0:18,2	1/1:35:4:.,.
+20	1230237	.	T	.	47	PASS	AA=T;DP=13;NS=3	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA	50	PASS	AA=G;AC=3,1;AN=6;DP=9;NS=3	GT:GQ:DP	0/1:.:4	./0:17:2	1/1:40:3
+20	1234567	microsat1	G	GAC	50	PASS	AA=G;AC=3,1;AN=6;DP=9;NS=3	GT:GQ:DP	./0:.:4	0/1:17:2	./.:40:3
+20	1235237	.	T	.	0	.	.	GT	0/0	0|0	.
+X	10	rsTest	AC	A	10	PASS	.	GT	0	0/1	./0
+X	10	rsTest	AC	ATG	10	PASS	.	GT	0	./0	0/1
diff --git a/tool_collections/vcflib/vcfbreakcreatemulti/test-data/vcfbreakcreatemulti-test2.vcf b/tool_collections/vcflib/vcfbreakcreatemulti/test-data/vcfbreakcreatemulti-test2.vcf
new file mode 100644
index 00000000000..c98893f361c
--- /dev/null
+++ b/tool_collections/vcflib/vcfbreakcreatemulti/test-data/vcfbreakcreatemulti-test2.vcf
@@ -0,0 +1,32 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+##INFO=<ID=combined,Number=1,Type=String,Description="Range of overlapping variants which were combined into this one using vcfcreatemulti.">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	AF=0.5;DP=14;NS=3;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	AF=0.017;DP=11;NS=3	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	AA=T;AF=0.333,0.667;DP=10;NS=2;combined=1110696-1110696;DB	GT:GQ:DP:HQ	./1:21:6:23,27	./1:2:0:18,2	./.:35:4:.,.
+20	1230237	.	T	.	47	PASS	AA=T;DP=13;NS=3	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	AA=G;AC=3,1;AN=6;DP=9;NS=3;combined=1234567-1234567	GT:GQ:DP	0/1:.:4	./0:17:2	1/1:40:3
+20	1235237	.	T	.	0	.	.	GT	0/0	0|0	.
+X	10	rsTest	AC	A,ATG	10	PASS	combined=10-10	GT	0	0/1	./0
diff --git a/tool_collections/vcflib/vcfbreakcreatemulti/test-data/vcflib.vcf b/tool_collections/vcflib/vcfbreakcreatemulti/test-data/vcflib.vcf
new file mode 100644
index 00000000000..e8dd794d9bd
--- /dev/null
+++ b/tool_collections/vcflib/vcfbreakcreatemulti/test-data/vcflib.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	NS=3;DP=14;AF=0.5;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	NS=3;DP=11;AF=0.017	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	NS=2;DP=10;AF=0.333,0.667;AA=T;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	NS=3;DP=13;AA=T	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	NS=3;DP=9;AA=G;AN=6;AC=3,1	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	.	.	.	GT	0/0	0|0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcfbreakcreatemulti/vcfbreakcreatemulti.xml b/tool_collections/vcflib/vcfbreakcreatemulti/vcfbreakcreatemulti.xml
new file mode 100644
index 00000000000..58eaf85afcb
--- /dev/null
+++ b/tool_collections/vcflib/vcfbreakcreatemulti/vcfbreakcreatemulti.xml
@@ -0,0 +1,48 @@
+<tool id="vcfbreakcreatemulti" name="VCFbreakCreateMulti:" version="@WRAPPER_VERSION@.0">
+    <description>Break multiple alleles into multiple records, or combine overallpoing alleles into a single record</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"/>
+    <expand macro="stdio" />
+    <command>
+        #if str($break_or_create) == "break":
+            vcfbreakmulti "${input1}" > "${out_file1}"
+        #elif str($break_or_create) == "create":
+            vcfcreatemulti "${input1}" > "${out_file1}"
+        #end if
+    </command>
+    <inputs>
+        <param format="vcf" name="input1" type="data" label="Select VCF dataset"/>
+        <param name="break_or_create" type="select" display="radio" label="Break or Create?">
+            <option value="break">Break</option>
+            <option value="create">Create</option>
+        </param>
+    </inputs>
+    <outputs>
+        <data format="vcf" name="out_file1" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="break_or_create" value="break"/>
+            <param name="input1" value="vcflib.vcf"/>
+            <output name="out_file1" file="vcfbreakcreatemulti-test1.vcf"/>
+        </test>
+        <test>
+            <param name="break_or_create" value="create"/>
+            <param name="input1" value="vcfbreakcreatemulti-test2-input.vcf"/>
+            <output name="out_file1" file="vcfbreakcreatemulti-test2.vcf"/>
+        </test>
+    </tests>
+    <help>
+This tool breaks or creates multiallelic VCF records based on user selection (**Break** or **Create**, respectively):
+
+ - **Break** = If multiple alleles are specified in a single record, break the record into multiple lines, preserving allele-specific INFO fields.
+ - **Create** = If overlapping alleles are represented across multiple records, merge them into a single record.
+
+----
+
+This tools is based on vcfbreakmulti and vcfcreatemulti utilities from the VCFlib toolkit developed by Erik Garrison (https://github.com/ekg/vcflib).
+    </help>
+    <expand macro="citations" />
+</tool>
diff --git a/tool_collections/vcflib/vcfcheck/.shed.yml b/tool_collections/vcflib/vcfcheck/.shed.yml
new file mode 100644
index 00000000000..32686dc3ed5
--- /dev/null
+++ b/tool_collections/vcflib/vcfcheck/.shed.yml
@@ -0,0 +1,10 @@
+categories:
+- Variant Analysis
+description: Verify that the reference allele matches the reference genome
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  Verifies that the VCF REF field matches the reference as described
+name: vcfcheck
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfcheck
+type: unrestricted
diff --git a/tool_collections/vcflib/vcfcheck/macros.xml b/tool_collections/vcflib/vcfcheck/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcfcheck/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfcheck/test-data/vcfcheck-test1.vcf b/tool_collections/vcflib/vcfcheck/test-data/vcfcheck-test1.vcf
new file mode 100644
index 00000000000..362e6be2088
--- /dev/null
+++ b/tool_collections/vcflib/vcfcheck/test-data/vcfcheck-test1.vcf
@@ -0,0 +1,219 @@
+##fileformat=VCFv4.1
+##fileDate=20140610
+##source=Dan
+##reference=file:///galaxy/data/phiX/sam_index/phiX.fa
+##filter="AF > .001"
+##INFO=<ID=AC,Number=A,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=AF,Number=A,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=AF,Number=.,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=NC,Number=.,Type=String,Description="Nucleotide and indel counts">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	groupA	groupB
+phiX174	1015	.	A	C	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:0,2:0,0.2:A=8,C=2,G=1,	0:0,0:0,0:A=26,G=1,
+phiX174	1016	.	A	T	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:0:0:A=10,	0:2:0.0769230769231:A=24,T=2,
+phiX174	1038	.	A	C	0	.	AC=2;AF=0.046511627907	GT:AC:AF:NC	0:2:0.153846153846:A=11,C=2,	0:0:0:A=30,
+phiX174	1045	.	T	G	0	.	AC=2;AF=0.0425531914894	GT:AC:AF:NC	0:0:0:T=15,G=1,	0:2:0.0625:T=30,G=2,
+phiX174	1048	.	C	A	0	.	AC=2;AF=0.0434782608696	GT:AC:AF:NC	0:2:0.133333333333:A=2,C=13,	0:0:0:C=31,G=1,
+phiX174	1050	.	A	C	0	.	AC=3;AF=0.0714285714286	GT:AC:AF:NC	0:0:0:A=12,	0:3:0.1:A=27,C=3,
+phiX174	1051	.	T	G	0	.	AC=2;AF=0.0487804878049	GT:AC:AF:NC	0:2:0.166666666667:C=1,T=10,G=2,	0:0:0:T=29,G=1,
+phiX174	1054	.	T	G	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:2:0.153846153846:T=11,G=2,	0:0:0:T=31,G=1,
+phiX174	1076	.	G	C	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=2,G=17,	0:0:0:G=36,
+phiX174	1080	.	G	C	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=2,T=1,G=17,	0:0:0:C=1,G=39,
+phiX174	1092	.	T	G	0	.	AC=2;AF=0.0263157894737	GT:AC:AF:NC	0:2:0.0833333333333:C=1,T=22,G=2,	0:0:0:T=52,G=1,
+phiX174	1093	.	T	G	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.08:T=23,G=2,	0:0:0:T=52,
+phiX174	1102	.	T	C	0	.	AC=6;AF=0.0779220779221	GT:AC:AF:NC	0:2:0.0869565217391:C=2,T=21,	0:4:0.0740740740741:C=4,T=50,
+phiX174	1107	.	T	G	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:0:0:T=22,G=1,	0:2:0.04:T=48,G=2,
+phiX174	1110	.	T	C	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:2:0.125:C=2,T=14,	0:0:0:C=1,T=56,G=1,
+phiX174	1121	.	T	G	0	.	AC=3;AF=0.041095890411	GT:AC:AF:NC	0:0:0:T=19,	0:3:0.0555555555556:T=51,G=3,
+phiX174	1124	.	T	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0,2:0,0.117647058824:C=2,T=15,G=1,	0:0,0:0,0:T=48,G=1,
+phiX174	1125	.	T	G	0	.	AC=3;AF=0.0454545454545	GT:AC:AF:NC	0:3:0.176470588235:T=14,G=3,	0:0:0:T=49,G=1,
+phiX174	1131	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0952380952381:T=19,G=2,	0:0:0:T=57,
+phiX174	1141	.	T	G	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0:0:T=20,G=1,	0:2:0.0338983050847:T=57,G=2,
+phiX174	1145	.	G	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.0952380952381:C=2,G=19,	0:0:0:G=52,
+phiX174	1147	.	T	C	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0952380952381:C=2,T=19,	0:0:0:T=50,
+phiX174	1149	.	T	G,C	0	.	AC=2,2;AF=0.027027027027,0.027027027027	GT:AC:AF:NC	0:2,2:0.1,0.1:C=2,T=16,G=2,	0:0,0:0,0:T=54,
+phiX174	1156	.	T	G	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:0:0:C=1,T=15,	0:2:0.0416666666667:T=46,G=2,
+phiX174	1159	.	T	C,G	0	.	AC=2,2;AF=0.0298507462687,0.0298507462687	GT:AC:AF:NC	0:2,0:0.111111111111,0:C=2,T=16,	0:0,2:0,0.0408163265306:C=1,T=47,G=2,
+phiX174	1164	.	T	G	0	.	AC=4;AF=0.0666666666667	GT:AC:AF:NC	0:0:0:C=1,T=16,G=1,	0:4:0.0909090909091:T=40,G=4,
+phiX174	1168	.	T	G	0	.	AC=4;AF=0.0666666666667	GT:AC:AF:NC	0:2:0.0952380952381:C=1,T=19,G=2,	0:2:0.0512820512821:T=37,G=2,
+phiX174	1171	.	T	G	0	.	AC=2;AF=0.0327868852459	GT:AC:AF:NC	0:2:0.0869565217391:T=21,G=2,	0:0:0:T=38,G=1,
+phiX174	1174	.	C	A	0	.	AC=2;AF=0.0327868852459	GT:AC:AF:NC	0:2:0.0869565217391:A=2,C=21,	0:0:0:A=1,C=38,
+phiX174	1178	.	G	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:2:0.0740740740741:C=2,T=1,G=25,	0:0:0:G=38,
+phiX174	1187	.	T	G	0	.	AC=3;AF=0.04	GT:AC:AF:NC	0:3:0.0882352941176:C=1,T=31,G=3,	0:0:0:T=41,
+phiX174	1189	.	T	C	0	.	AC=3;AF=0.0379746835443	GT:AC:AF:NC	0:3:0.0857142857143:C=3,T=32,G=1,	0:0:0:T=44,
+phiX174	1200	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0882352941176:T=31,G=3,	0:0:0:T=48,G=1,
+phiX174	1202	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:0:0:T=32,	0:3:0.06:T=47,G=3,
+phiX174	1205	.	A	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.0588235294118:A=32,C=2,	0:0:0:A=47,C=1,G=1,
+phiX174	1207	.	T	G	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:A=1,T=34,	0:2:0.0425531914894:T=45,G=2,
+phiX174	1214	.	G	A	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:T=1,G=34,	0:2:0.0425531914894:A=2,G=45,
+phiX174	1216	.	C	A	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:C=34,	0:2:0.0425531914894:A=2,C=45,
+phiX174	1217	.	C	A	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.0588235294118:A=2,C=32,	0:0:0:C=46,
+phiX174	1219	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:0:0:C=1,T=33,G=1,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1223	.	C	T	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.0555555555556:C=34,T=2,	0:0:0:C=44,
+phiX174	1227	.	A	C	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0588235294118:A=32,C=2,	0:0:0:A=40,
+phiX174	1232	.	T	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:T=34,	0:2:0.0606060606061:C=1,T=31,G=2,
+phiX174	1236	.	G	T	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0606060606061:A=1,T=2,G=31,	0:0:0:G=35,
+phiX174	1237	.	T	G	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=1,T=33,G=2,	0:0:0:T=33,G=1,
+phiX174	1240	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:0,0:0,0:A=32,T=1,	0:0,2:0,0.0555555555556:A=34,C=2,T=1,
+phiX174	1245	.	G	T	0	.	AC=4;AF=0.0625	GT:AC:AF:NC	0:2:0.0666666666667:C=1,T=2,G=28,	0:2:0.0588235294118:T=2,G=32,
+phiX174	1247	.	A	T	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:2:0.0714285714286:A=26,C=1,T=2,G=1,	0:0:0:A=35,
+phiX174	1250	.	A	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:2:0.0769230769231:A=24,C=2,	0:0:0:A=39,
+phiX174	1251	.	A	G	0	.	AC=3;AF=0.044776119403	GT:AC:AF:NC	0:0:0:A=29,	0:3:0.0789473684211:A=35,C=1,G=3,
+phiX174	1253	.	T	G	0	.	AC=4;AF=0.0526315789474	GT:AC:AF:NC	0:2:0.0645161290323:T=29,G=2,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1257	.	T	G	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:0:0:T=26,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1259	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:A=28,T=1,	0:2:0.0444444444444:A=43,C=2,G=1,
+phiX174	1274	.	G	C	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.0512820512821:C=2,G=37,	0:0:0:G=44,
+phiX174	1277	.	A	C	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=40,G=1,
+phiX174	1279	.	T	G	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0:0:T=39,G=1,	0:2:0.05:C=1,T=38,G=2,
+phiX174	1284	.	T	G,C	0	.	AC=3,2;AF=0.038961038961,0.025974025974	GT:AC:AF:NC	0:3,2:0.075,0.05:C=2,T=35,G=3,	0:0,0:0,0:A=1,T=37,
+phiX174	1289	.	A	C	0	.	AC=3;AF=0.0454545454545	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=28,G=1,
+phiX174	1296	.	A	C	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:0:0:A=38,	0:2:0.0769230769231:A=24,C=2,
+phiX174	1298	.	A	C	0	.	AC=3;AF=0.0491803278689	GT:AC:AF:NC	0:3,0:0.0810810810811,0:A=34,C=3,G=1,	0:0,0:0,0:A=24,C=1,T=1,G=1,
+phiX174	1306	.	T	G	0	.	AC=5;AF=0.0943396226415	GT:AC:AF:NC	0:5:0.178571428571:T=23,G=5,	0:0:0:C=1,T=25,
+phiX174	1307	.	T	G	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.107142857143:T=25,G=3,	0:0:0:C=1,T=27,
+phiX174	1314	.	A	G	0	.	AC=5;AF=0.0877192982456	GT:AC:AF:NC	0:2,0:0.0740740740741,0:A=25,C=1,G=2,	0:3,0:0.1,0:A=27,C=1,G=3,
+phiX174	1317	.	A	C	0	.	AC=2;AF=0.0322580645161	GT:AC:AF:NC	0:2:0.0740740740741:A=25,C=2,	0:0:0:A=35,
+phiX174	1320	.	C	G	0	.	AC=2;AF=0.0322580645161	GT:AC:AF:NC	0:2:0.0769230769231:C=24,G=2,	0:0:0:C=36,
+phiX174	1322	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.107142857143:C=3,G=25,	0:0:0:G=40,
+phiX174	1323	.	C	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:C=28,	0:2:0.0512820512821:C=37,T=1,G=2,
+phiX174	1329	.	T	C	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:2:0.0666666666667:C=2,T=28,	0:0:0:T=42,G=1,
+phiX174	1335	.	C	A	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.0625:A=2,C=30,	0:0:0:C=45,
+phiX174	1338	.	T	A	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:0:0:T=32,	0:2:0.047619047619:A=2,T=40,
+phiX174	1345	.	T	C	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0,0:0,0:T=36,G=1,	0:0,2:0,0.046511627907:C=2,T=41,G=1,
+phiX174	1347	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0512820512821:A=37,C=2,G=1,	0:0:0:A=43,
+phiX174	1348	.	T	G	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.05:T=38,G=2,	0:0:0:T=43,
+phiX174	1352	.	A	T	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:A=34,G=1,	0:2:0.0512820512821:A=37,T=2,
+phiX174	1354	.	T	G,A	0	.	AC=2,2;AF=0.027027027027,0.027027027027	GT:AC:AF:NC	0:2,0:0.0526315789474,0:T=36,G=2,	0:0,2:0,0.0555555555556:A=2,T=34,G=1,
+phiX174	1359	.	T	G	0	.	AC=3;AF=0.0405405405405	GT:AC:AF:NC	0:3:0.0714285714286:T=39,G=3,	0:0:0:T=32,G=1,
+phiX174	1366	.	G	C	0	.	AC=3;AF=0.0428571428571	GT:AC:AF:NC	0:3:0.0714285714286:C=3,T=1,G=39,	0:0:0:G=28,
+phiX174	1370	.	T	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:0:0:C=1,T=45,G=1,	0:2:0.0833333333333:A=1,T=22,G=2,
+phiX174	1373	.	T	G	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0408163265306:T=47,G=2,	0:0:0:T=25,
+phiX174	1380	.	G	C	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:0,2:0,0.0384615384615:C=2,T=1,G=50,	0:0,0:0,0:T=1,G=32,
+phiX174	1381	.	T	G	0	.	AC=3;AF=0.0361445783133	GT:AC:AF:NC	0:3:0.0576923076923:A=1,T=49,G=3,	0:0:0:T=31,
+phiX174	1384	.	T	G	0	.	AC=4;AF=0.0512820512821	GT:AC:AF:NC	0:4:0.0816326530612:T=45,G=4,	0:0:0:T=29,G=1,
+phiX174	1385	.	T	G	0	.	AC=4;AF=0.0512820512821	GT:AC:AF:NC	0:4:0.0816326530612:T=45,G=4,	0:0:0:C=1,T=29,
+phiX174	1391	.	A	C	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:2:0.0408163265306:A=47,C=2,	0:0:0:A=30,C=1,
+phiX174	1393	.	C	A	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:2:0.0425531914894:A=2,C=45,	0:0:0:C=32,
+phiX174	1396	.	T	G	0	.	AC=3;AF=0.04	GT:AC:AF:NC	0:3:0.0697674418605:T=40,G=3,	0:0:0:T=32,
+phiX174	1397	.	A	C	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:0:0:A=42,T=1,	0:2:0.0625:A=30,C=2,
+phiX174	1400	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.047619047619:A=40,C=2,G=1,	0:0:0:A=31,
+phiX174	1401	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.047619047619:A=40,C=2,	0:0:0:A=31,
+phiX174	1434	.	C	T	0	.	AC=2;AF=0.0571428571429	GT:AC:AF:NC	0:2:0.142857142857:C=12,T=2,	0:0:0:C=21,
+phiX174	1438	.	G	C	0	.	AC=2;AF=0.0526315789474	GT:AC:AF:NC	0:0:0:G=15,	0:2:0.0869565217391:C=2,G=21,
+phiX174	1450	.	T	G	0	.	AC=3;AF=0.0789473684211	GT:AC:AF:NC	0:3:0.176470588235:A=1,C=1,T=14,G=3,	0:0:0:T=21,G=1,
+phiX174	1459	.	T	G	0	.	AC=3;AF=0.075	GT:AC:AF:NC	0:3:0.142857142857:C=1,T=18,G=3,	0:0:0:T=19,
+phiX174	1470	.	C	A	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:0:0:C=29,	0:2:0.0869565217391:A=2,C=21,
+phiX174	1484	.	C	G	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:2:0.0740740740741:A=1,C=25,G=2,	0:0:0:C=25,
+phiX174	1487	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0689655172414:T=27,G=2,	0:0:0:T=26,
+phiX174	1491	.	G	T	0	.	AC=2;AF=0.037037037037	GT:AC:AF:NC	0:0:0:T=1,G=26,	0:2:0.0714285714286:T=2,G=26,
+phiX174	1503	.	A	C	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:0:0:A=20,C=1,	0:2:0.0833333333333:A=22,C=2,
+phiX174	1536	.	A	C	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.111111111111:A=24,C=3,	0:0:0:A=28,C=1,
+phiX174	1541	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0740740740741:T=25,G=2,	0:0:0:T=28,
+phiX174	1553	.	A	G	0	.	AC=2;AF=0.0408163265306	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=1,G=2,	0:0:0:A=28,
+phiX174	1566	.	C	G	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0:0:C=30,T=1,	0:2:0.0571428571429:C=33,G=2,
+phiX174	1572	.	T	G	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:2:0.0625:T=30,G=2,	0:0:0:T=32,
+phiX174	1574	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0882352941176:C=3,G=31,	0:0:0:G=34,
+phiX174	1575	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=33,T=1,
+phiX174	1577	.	A	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:0:0:A=35,	0:3:0.0909090909091:A=30,C=3,
+phiX174	1581	.	T	G	0	.	AC=2;AF=0.0285714285714	GT:AC:AF:NC	0:2:0.0555555555556:C=1,T=34,G=2,	0:0:0:T=34,G=1,
+phiX174	1591	.	A	C	0	.	AC=3;AF=0.037037037037	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=43,C=1,
+phiX174	1597	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0606060606061:T=31,G=2,	0:0:0:C=1,T=45,
+phiX174	1603	.	T	G	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0625:T=30,G=2,	0:0:0:T=50,
+phiX174	1606	.	T	G	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0666666666667:A=1,C=1,T=28,G=2,	0:0:0:T=52,
+phiX174	1611	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0769230769231:A=24,C=2,	0:0:0:A=56,C=1,
+phiX174	1613	.	A	G	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:0:0:A=25,C=1,G=1,	0:2:0.0344827586207:A=56,G=2,
+phiX174	1618	.	C	A	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0666666666667:A=2,C=28,G=1,	0:0:0:A=1,C=57,
+phiX174	1619	.	C	A	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0666666666667:A=2,C=28,	0:0:0:C=57,
+phiX174	1621	.	A	C	0	.	AC=3;AF=0.0352941176471	GT:AC:AF:NC	0:0:0:A=31,T=1,G=1,	0:3:0.0555555555556:A=51,C=3,
+phiX174	1622	.	G	T	0	.	AC=2;AF=0.0224719101124	GT:AC:AF:NC	0:0:0:A=1,G=35,	0:2:0.037037037037:T=2,G=52,
+phiX174	1624	.	A	G	0	.	AC=2;AF=0.0235294117647	GT:AC:AF:NC	0:2:0.0555555555556:A=34,T=1,G=2,	0:0:0:A=49,
+phiX174	1627	.	T	G,C	0	.	AC=2,2;AF=0.0246913580247,0.0246913580247	GT:AC:AF:NC	0:0,0:0,0:T=37,G=1,	0:2,2:0.0454545454545,0.0454545454545:C=2,T=40,G=2,
+phiX174	1630	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0526315789474:C=1,T=36,G=2,	0:0:0:T=40,
+phiX174	1632	.	A	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=41,
+phiX174	1634	.	T	G	0	.	AC=4;AF=0.0493827160494	GT:AC:AF:NC	0:2:0.047619047619:C=1,T=40,G=2,	0:2:0.0512820512821:T=37,G=2,
+phiX174	1635	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0714285714286:T=39,G=3,	0:0:0:T=40,
+phiX174	1639	.	G	A	0	.	AC=3;AF=0.0405405405405	GT:AC:AF:NC	0:3:0.075:A=3,T=1,G=37,	0:0:0:G=34,
+phiX174	1645	.	T	G	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0425531914894:T=45,G=2,	0:0:0:T=27,G=1,
+phiX174	1647	.	A	T,G,C	0	.	AC=2,2,2;AF=0.0253164556962,0.0253164556962,0.0253164556962	GT:AC:AF:NC	0:0,2,2:0,0.0384615384615,0.0384615384615:A=48,C=2,G=2,	0:2,0,0:0.0740740740741,0,0:A=25,T=2,
+phiX174	1652	.	G	A	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.037037037037:A=2,G=52,	0:0:0:G=29,
+phiX174	1656	.	T	G	0	.	AC=3;AF=0.0348837209302	GT:AC:AF:NC	0:3:0.0555555555556:T=51,G=3,	0:0:0:T=32,G=1,
+phiX174	1658	.	A	C	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.04:A=48,C=2,	0:0:0:A=33,
+phiX174	1659	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0625:C=1,T=45,G=3,	0:0:0:T=34,
+phiX174	1663	.	T	G	0	.	AC=4;AF=0.0454545454545	GT:AC:AF:NC	0:0:0:A=1,T=53,	0:4:0.114285714286:T=31,G=4,
+phiX174	1665	.	C	A	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0384615384615:A=2,C=50,G=1,	0:0:0:C=42,
+phiX174	1670	.	G	C	0	.	AC=2;AF=0.0224719101124	GT:AC:AF:NC	0:0,2:0,0.0408163265306:A=1,C=2,T=1,G=47,	0:0,0:0,0:T=1,G=40,
+phiX174	1671	.	G	T	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0384615384615:C=1,T=2,G=50,	0:0:0:G=42,
+phiX174	1672	.	A	G	0	.	AC=3;AF=0.0315789473684	GT:AC:AF:NC	0:3:0.0545454545455:A=52,G=3,	0:0:0:A=40,T=1,
+phiX174	1673	.	G	T	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0377358490566:C=1,T=2,G=51,	0:0:0:G=41,
+phiX174	1674	.	G	T	0	.	AC=4;AF=0.04	GT:AC:AF:NC	0:4:0.0714285714286:T=4,G=52,	0:0:0:G=44,
+phiX174	1677	.	A	C	0	.	AC=3;AF=0.031914893617	GT:AC:AF:NC	0:3:0.0566037735849:A=50,C=3,N=1,	0:0:0:A=41,
+phiX174	1678	.	A	C	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:0:0:A=54,C=1,G=1,	0:2:0.05:A=38,C=2,
+phiX174	1682	.	T	C	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0408163265306:C=2,T=47,G=1,	0:0:0:C=1,T=38,
+phiX174	1685	.	T	G	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:2:0.046511627907:A=1,T=41,G=2,	0:0:0:T=41,G=1,
+phiX174	1686	.	A	C	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:0:0:A=39,C=1,T=1,G=1,	0:2:0.0512820512821:A=37,C=2,
+phiX174	1687	.	T	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.047619047619:C=2,T=40,	0:0:0:A=1,T=40,
+phiX174	1688	.	G	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.05:C=2,T=1,G=38,	0:0:0:G=41,
+phiX174	1689	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0487804878049:A=39,C=2,T=1,G=1,	0:0:0:A=41,C=1,
+phiX174	1691	.	G	C	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.05:C=2,T=1,G=38,	0:0:0:G=37,
+phiX174	1693	.	T	G	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:0:0:T=42,G=1,	0:2:0.047619047619:T=40,G=2,
+phiX174	1695	.	A	C	0	.	AC=2;AF=0.0232558139535	GT:AC:AF:NC	0:2:0.046511627907:A=41,C=2,	0:0:0:A=43,C=1,
+phiX174	1702	.	T	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.0512820512821:A=1,C=2,T=37,	0:0:0:C=1,T=42,
+phiX174	1707	.	T	C	0	.	AC=2;AF=0.0232558139535	GT:AC:AF:NC	0:2:0.05:C=2,T=38,	0:0:0:T=46,G=1,
+phiX174	1708	.	A	C	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:2:0.0526315789474:A=36,C=2,	0:0:0:A=46,C=1,G=1,
+phiX174	1710	.	T	C	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:0:0:C=1,T=34,	0:2:0.0434782608696:C=2,T=44,
+phiX174	1723	.	T	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0588235294118:C=2,T=32,	0:0:0:T=48,
+phiX174	1724	.	A	C	0	.	AC=4;AF=0.0481927710843	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:2:0.0416666666667:A=46,C=2,
+phiX174	1725	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0606060606061:A=31,C=2,	0:0:0:A=49,
+phiX174	1726	.	T	G	0	.	AC=3;AF=0.037037037037	GT:AC:AF:NC	0:3:0.0882352941176:T=31,G=3,	0:0:0:C=1,T=47,
+phiX174	1730	.	T	G	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:C=1,T=28,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1739	.	G	T	0	.	AC=3;AF=0.0434782608696	GT:AC:AF:NC	0:3:0.09375:T=3,G=29,	0:0:0:G=37,
+phiX174	1746	.	A	C	0	.	AC=2;AF=0.0338983050847	GT:AC:AF:NC	0:2:0.0689655172414:A=27,C=2,	0:0:0:A=30,C=1,G=1,
+phiX174	1749	.	T	G,C	0	.	AC=3,2;AF=0.044776119403,0.0298507462687	GT:AC:AF:NC	0:3,2:0.0857142857143,0.0571428571429:C=2,T=30,G=3,	0:0,0:0,0:T=32,G=1,
+phiX174	1755	.	G	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.05:C=2,G=38,	0:0:0:C=1,G=33,
+phiX174	1756	.	A	G,C	0	.	AC=2,2;AF=0.0281690140845,0.0281690140845	GT:AC:AF:NC	0:0,2:0,0.0526315789474:A=36,C=2,T=1,G=1,	0:2,0:0.0606060606061,0:A=31,C=1,G=2,
+phiX174	1761	.	A	G	0	.	AC=2;AF=0.0285714285714	GT:AC:AF:NC	0:2:0.0512820512821:A=37,C=1,G=2,	0:0:0:A=31,
+phiX174	1764	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=28,
+phiX174	1766	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0487804878049:A=39,C=2,	0:0:0:A=27,
+phiX174	1769	.	T	G	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:2:0.0526315789474:A=1,C=1,T=36,G=2,	0:0:0:T=26,G=1,
+phiX174	1771	.	G	C	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:2:0.0526315789474:C=2,G=36,	0:0:0:G=29,
+phiX174	1772	.	T	G	0	.	AC=4;AF=0.0597014925373	GT:AC:AF:NC	0:4:0.108108108108:C=1,T=33,G=4,	0:0:0:T=30,
+phiX174	1774	.	A	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0810810810811:A=34,G=3,	0:0:0:A=31,
+phiX174	1775	.	G	A	0	.	AC=2;AF=0.030303030303	GT:AC:AF:NC	0:2:0.0571428571429:A=2,G=33,	0:0:0:G=31,
+phiX174	1776	.	G	T	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0540540540541:T=2,G=35,	0:0:0:G=32,
+phiX174	1778	.	C	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0526315789474:C=36,G=2,	0:0:0:C=31,
+phiX174	1779	.	A	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.075:A=37,C=3,	0:0:0:A=28,
+phiX174	1781	.	T	G	0	.	AC=5;AF=0.0714285714286	GT:AC:AF:NC	0:2:0.0487804878049:C=1,T=39,G=2,	0:3:0.103448275862:T=26,G=3,
+phiX174	1782	.	T	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.075:T=37,G=3,	0:0:0:A=1,T=28,
+phiX174	1783	.	T	C	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:2:0.0512820512821:C=2,T=37,	0:0:0:T=28,G=1,
+phiX174	1788	.	G	T	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:0:0:A=1,T=1,G=29,	0:2:0.0689655172414:T=2,G=27,
+phiX174	1792	.	T	G	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:2:0.0606060606061:T=31,G=2,	0:0:0:T=25,G=1,
+phiX174	1802	.	A	C	0	.	AC=2;AF=0.030303030303	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=31,C=1,
+phiX174	1804	.	C	T	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0588235294118:A=1,C=32,T=2,	0:0:0:C=34,
+phiX174	1805	.	T	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0857142857143:T=32,G=3,	0:0:0:T=33,G=1,
+phiX174	1806	.	A	C	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=34,G=1,
+phiX174	1824	.	G	C	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0714285714286:C=2,T=1,G=26,	0:0:0:G=43,
+phiX174	1825	.	T	G	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0689655172414:T=27,G=2,	0:0:0:T=42,G=1,
+phiX174	1833	.	T	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:0:0:A=1,T=24,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1843	.	T	G	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:2:0.08:A=1,T=23,G=2,	0:0:0:T=38,
+phiX174	1847	.	A	C	0	.	AC=2;AF=0.0333333333333	GT:AC:AF:NC	0:2:0.0833333333333:A=22,C=2,T=1,G=1,	0:0:0:A=36,
+phiX174	1854	.	T	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:T=26,	0:2:0.0487804878049:T=39,G=2,
+phiX174	1860	.	T	G	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:0:0:A=1,T=20,	0:2:0.0606060606061:T=31,G=2,
+phiX174	1863	.	C	G	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=17,G=2,	0:0:0:C=33,
+phiX174	1866	.	T	G	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:0:0:T=19,G=1,	0:2:0.0645161290323:T=29,G=2,
+phiX174	1875	.	T	A	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:2:0.0952380952381:A=2,T=19,	0:0:0:T=32,G=1,
+phiX174	1876	.	T	G	0	.	AC=3;AF=0.0588235294118	GT:AC:AF:NC	0:3:0.157894736842:C=1,T=16,G=3,	0:0:0:T=32,G=1,
+phiX174	1882	.	T	G	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:2:0.105263157895:T=17,G=2,	0:0:0:T=34,
+phiX174	1889	.	A	C	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:2:0.0869565217391:A=21,C=2,	0:0:0:A=27,
+phiX174	1913	.	G	C	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:0:0:G=27,	0:2:0.0869565217391:C=2,G=21,
+phiX174	1915	.	T	G	0	.	AC=2;AF=0.0425531914894	GT:AC:AF:NC	0:0:0:A=1,T=24,	0:2:0.0869565217391:T=21,G=2,
+phiX174	1917	.	A	T	0	.	AC=2;AF=0.0444444444444	GT:AC:AF:NC	0:2,0:0.0833333333333,0:A=22,T=2,G=1,	0:0,0:0,0:A=21,G=1,
+phiX174	1930	.	T	A	0	.	AC=2;AF=0.0526315789474	GT:AC:AF:NC	0:2:0.0909090909091:A=2,T=20,	0:0:0:T=16,
+phiX174	1934	.	A	C	0	.	AC=2;AF=0.0512820512821	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=2,T=1,	0:0:0:A=18,C=1,
+phiX174	1940	.	A	C	0	.	AC=2;AF=0.046511627907	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=2,	0:0:0:A=22,C=1,
+phiX174	1942	.	T	G	0	.	AC=2;AF=0.047619047619	GT:AC:AF:NC	0:0:0:T=19,	0:2:0.0869565217391:T=21,G=2,
+phiX174	1945	.	T	G	0	.	AC=3;AF=0.0769230769231	GT:AC:AF:NC	0:0:0:T=15,G=1,	0:3:0.125:T=21,G=3,
+phiX174	1954	.	T	C	0	.	AC=2;AF=0.0625	GT:AC:AF:NC	0:0,0:0,0:T=12,G=1,	0:0,2:0,0.1:C=2,T=18,G=1,
+phiX174	1959	.	T	G	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:2:0.153846153846:T=11,G=2,	0:0:0:T=23,
+phiX174	1994	.	A	C	0	.	AC=2;AF=0.0512820512821	GT:AC:AF:NC	0:2:0.153846153846:A=11,C=2,	0:0:0:A=26,
+phiX174	1998	.	A	G	0	.	AC=2;AF=0.05	GT:AC:AF:NC	0:2:0.142857142857:A=12,G=2,	0:0:0:A=26,
diff --git a/tool_collections/vcflib/vcfcheck/test-data/vcflib-phix.vcf b/tool_collections/vcflib/vcfcheck/test-data/vcflib-phix.vcf
new file mode 100644
index 00000000000..362e6be2088
--- /dev/null
+++ b/tool_collections/vcflib/vcfcheck/test-data/vcflib-phix.vcf
@@ -0,0 +1,219 @@
+##fileformat=VCFv4.1
+##fileDate=20140610
+##source=Dan
+##reference=file:///galaxy/data/phiX/sam_index/phiX.fa
+##filter="AF > .001"
+##INFO=<ID=AC,Number=A,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=AF,Number=A,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=AF,Number=.,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=NC,Number=.,Type=String,Description="Nucleotide and indel counts">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	groupA	groupB
+phiX174	1015	.	A	C	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:0,2:0,0.2:A=8,C=2,G=1,	0:0,0:0,0:A=26,G=1,
+phiX174	1016	.	A	T	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:0:0:A=10,	0:2:0.0769230769231:A=24,T=2,
+phiX174	1038	.	A	C	0	.	AC=2;AF=0.046511627907	GT:AC:AF:NC	0:2:0.153846153846:A=11,C=2,	0:0:0:A=30,
+phiX174	1045	.	T	G	0	.	AC=2;AF=0.0425531914894	GT:AC:AF:NC	0:0:0:T=15,G=1,	0:2:0.0625:T=30,G=2,
+phiX174	1048	.	C	A	0	.	AC=2;AF=0.0434782608696	GT:AC:AF:NC	0:2:0.133333333333:A=2,C=13,	0:0:0:C=31,G=1,
+phiX174	1050	.	A	C	0	.	AC=3;AF=0.0714285714286	GT:AC:AF:NC	0:0:0:A=12,	0:3:0.1:A=27,C=3,
+phiX174	1051	.	T	G	0	.	AC=2;AF=0.0487804878049	GT:AC:AF:NC	0:2:0.166666666667:C=1,T=10,G=2,	0:0:0:T=29,G=1,
+phiX174	1054	.	T	G	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:2:0.153846153846:T=11,G=2,	0:0:0:T=31,G=1,
+phiX174	1076	.	G	C	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=2,G=17,	0:0:0:G=36,
+phiX174	1080	.	G	C	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=2,T=1,G=17,	0:0:0:C=1,G=39,
+phiX174	1092	.	T	G	0	.	AC=2;AF=0.0263157894737	GT:AC:AF:NC	0:2:0.0833333333333:C=1,T=22,G=2,	0:0:0:T=52,G=1,
+phiX174	1093	.	T	G	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.08:T=23,G=2,	0:0:0:T=52,
+phiX174	1102	.	T	C	0	.	AC=6;AF=0.0779220779221	GT:AC:AF:NC	0:2:0.0869565217391:C=2,T=21,	0:4:0.0740740740741:C=4,T=50,
+phiX174	1107	.	T	G	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:0:0:T=22,G=1,	0:2:0.04:T=48,G=2,
+phiX174	1110	.	T	C	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:2:0.125:C=2,T=14,	0:0:0:C=1,T=56,G=1,
+phiX174	1121	.	T	G	0	.	AC=3;AF=0.041095890411	GT:AC:AF:NC	0:0:0:T=19,	0:3:0.0555555555556:T=51,G=3,
+phiX174	1124	.	T	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0,2:0,0.117647058824:C=2,T=15,G=1,	0:0,0:0,0:T=48,G=1,
+phiX174	1125	.	T	G	0	.	AC=3;AF=0.0454545454545	GT:AC:AF:NC	0:3:0.176470588235:T=14,G=3,	0:0:0:T=49,G=1,
+phiX174	1131	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0952380952381:T=19,G=2,	0:0:0:T=57,
+phiX174	1141	.	T	G	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0:0:T=20,G=1,	0:2:0.0338983050847:T=57,G=2,
+phiX174	1145	.	G	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.0952380952381:C=2,G=19,	0:0:0:G=52,
+phiX174	1147	.	T	C	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0952380952381:C=2,T=19,	0:0:0:T=50,
+phiX174	1149	.	T	G,C	0	.	AC=2,2;AF=0.027027027027,0.027027027027	GT:AC:AF:NC	0:2,2:0.1,0.1:C=2,T=16,G=2,	0:0,0:0,0:T=54,
+phiX174	1156	.	T	G	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:0:0:C=1,T=15,	0:2:0.0416666666667:T=46,G=2,
+phiX174	1159	.	T	C,G	0	.	AC=2,2;AF=0.0298507462687,0.0298507462687	GT:AC:AF:NC	0:2,0:0.111111111111,0:C=2,T=16,	0:0,2:0,0.0408163265306:C=1,T=47,G=2,
+phiX174	1164	.	T	G	0	.	AC=4;AF=0.0666666666667	GT:AC:AF:NC	0:0:0:C=1,T=16,G=1,	0:4:0.0909090909091:T=40,G=4,
+phiX174	1168	.	T	G	0	.	AC=4;AF=0.0666666666667	GT:AC:AF:NC	0:2:0.0952380952381:C=1,T=19,G=2,	0:2:0.0512820512821:T=37,G=2,
+phiX174	1171	.	T	G	0	.	AC=2;AF=0.0327868852459	GT:AC:AF:NC	0:2:0.0869565217391:T=21,G=2,	0:0:0:T=38,G=1,
+phiX174	1174	.	C	A	0	.	AC=2;AF=0.0327868852459	GT:AC:AF:NC	0:2:0.0869565217391:A=2,C=21,	0:0:0:A=1,C=38,
+phiX174	1178	.	G	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:2:0.0740740740741:C=2,T=1,G=25,	0:0:0:G=38,
+phiX174	1187	.	T	G	0	.	AC=3;AF=0.04	GT:AC:AF:NC	0:3:0.0882352941176:C=1,T=31,G=3,	0:0:0:T=41,
+phiX174	1189	.	T	C	0	.	AC=3;AF=0.0379746835443	GT:AC:AF:NC	0:3:0.0857142857143:C=3,T=32,G=1,	0:0:0:T=44,
+phiX174	1200	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0882352941176:T=31,G=3,	0:0:0:T=48,G=1,
+phiX174	1202	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:0:0:T=32,	0:3:0.06:T=47,G=3,
+phiX174	1205	.	A	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.0588235294118:A=32,C=2,	0:0:0:A=47,C=1,G=1,
+phiX174	1207	.	T	G	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:A=1,T=34,	0:2:0.0425531914894:T=45,G=2,
+phiX174	1214	.	G	A	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:T=1,G=34,	0:2:0.0425531914894:A=2,G=45,
+phiX174	1216	.	C	A	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:C=34,	0:2:0.0425531914894:A=2,C=45,
+phiX174	1217	.	C	A	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.0588235294118:A=2,C=32,	0:0:0:C=46,
+phiX174	1219	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:0:0:C=1,T=33,G=1,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1223	.	C	T	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.0555555555556:C=34,T=2,	0:0:0:C=44,
+phiX174	1227	.	A	C	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0588235294118:A=32,C=2,	0:0:0:A=40,
+phiX174	1232	.	T	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:T=34,	0:2:0.0606060606061:C=1,T=31,G=2,
+phiX174	1236	.	G	T	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0606060606061:A=1,T=2,G=31,	0:0:0:G=35,
+phiX174	1237	.	T	G	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=1,T=33,G=2,	0:0:0:T=33,G=1,
+phiX174	1240	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:0,0:0,0:A=32,T=1,	0:0,2:0,0.0555555555556:A=34,C=2,T=1,
+phiX174	1245	.	G	T	0	.	AC=4;AF=0.0625	GT:AC:AF:NC	0:2:0.0666666666667:C=1,T=2,G=28,	0:2:0.0588235294118:T=2,G=32,
+phiX174	1247	.	A	T	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:2:0.0714285714286:A=26,C=1,T=2,G=1,	0:0:0:A=35,
+phiX174	1250	.	A	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:2:0.0769230769231:A=24,C=2,	0:0:0:A=39,
+phiX174	1251	.	A	G	0	.	AC=3;AF=0.044776119403	GT:AC:AF:NC	0:0:0:A=29,	0:3:0.0789473684211:A=35,C=1,G=3,
+phiX174	1253	.	T	G	0	.	AC=4;AF=0.0526315789474	GT:AC:AF:NC	0:2:0.0645161290323:T=29,G=2,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1257	.	T	G	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:0:0:T=26,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1259	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:A=28,T=1,	0:2:0.0444444444444:A=43,C=2,G=1,
+phiX174	1274	.	G	C	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.0512820512821:C=2,G=37,	0:0:0:G=44,
+phiX174	1277	.	A	C	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=40,G=1,
+phiX174	1279	.	T	G	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0:0:T=39,G=1,	0:2:0.05:C=1,T=38,G=2,
+phiX174	1284	.	T	G,C	0	.	AC=3,2;AF=0.038961038961,0.025974025974	GT:AC:AF:NC	0:3,2:0.075,0.05:C=2,T=35,G=3,	0:0,0:0,0:A=1,T=37,
+phiX174	1289	.	A	C	0	.	AC=3;AF=0.0454545454545	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=28,G=1,
+phiX174	1296	.	A	C	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:0:0:A=38,	0:2:0.0769230769231:A=24,C=2,
+phiX174	1298	.	A	C	0	.	AC=3;AF=0.0491803278689	GT:AC:AF:NC	0:3,0:0.0810810810811,0:A=34,C=3,G=1,	0:0,0:0,0:A=24,C=1,T=1,G=1,
+phiX174	1306	.	T	G	0	.	AC=5;AF=0.0943396226415	GT:AC:AF:NC	0:5:0.178571428571:T=23,G=5,	0:0:0:C=1,T=25,
+phiX174	1307	.	T	G	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.107142857143:T=25,G=3,	0:0:0:C=1,T=27,
+phiX174	1314	.	A	G	0	.	AC=5;AF=0.0877192982456	GT:AC:AF:NC	0:2,0:0.0740740740741,0:A=25,C=1,G=2,	0:3,0:0.1,0:A=27,C=1,G=3,
+phiX174	1317	.	A	C	0	.	AC=2;AF=0.0322580645161	GT:AC:AF:NC	0:2:0.0740740740741:A=25,C=2,	0:0:0:A=35,
+phiX174	1320	.	C	G	0	.	AC=2;AF=0.0322580645161	GT:AC:AF:NC	0:2:0.0769230769231:C=24,G=2,	0:0:0:C=36,
+phiX174	1322	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.107142857143:C=3,G=25,	0:0:0:G=40,
+phiX174	1323	.	C	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:C=28,	0:2:0.0512820512821:C=37,T=1,G=2,
+phiX174	1329	.	T	C	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:2:0.0666666666667:C=2,T=28,	0:0:0:T=42,G=1,
+phiX174	1335	.	C	A	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.0625:A=2,C=30,	0:0:0:C=45,
+phiX174	1338	.	T	A	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:0:0:T=32,	0:2:0.047619047619:A=2,T=40,
+phiX174	1345	.	T	C	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0,0:0,0:T=36,G=1,	0:0,2:0,0.046511627907:C=2,T=41,G=1,
+phiX174	1347	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0512820512821:A=37,C=2,G=1,	0:0:0:A=43,
+phiX174	1348	.	T	G	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.05:T=38,G=2,	0:0:0:T=43,
+phiX174	1352	.	A	T	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:A=34,G=1,	0:2:0.0512820512821:A=37,T=2,
+phiX174	1354	.	T	G,A	0	.	AC=2,2;AF=0.027027027027,0.027027027027	GT:AC:AF:NC	0:2,0:0.0526315789474,0:T=36,G=2,	0:0,2:0,0.0555555555556:A=2,T=34,G=1,
+phiX174	1359	.	T	G	0	.	AC=3;AF=0.0405405405405	GT:AC:AF:NC	0:3:0.0714285714286:T=39,G=3,	0:0:0:T=32,G=1,
+phiX174	1366	.	G	C	0	.	AC=3;AF=0.0428571428571	GT:AC:AF:NC	0:3:0.0714285714286:C=3,T=1,G=39,	0:0:0:G=28,
+phiX174	1370	.	T	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:0:0:C=1,T=45,G=1,	0:2:0.0833333333333:A=1,T=22,G=2,
+phiX174	1373	.	T	G	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0408163265306:T=47,G=2,	0:0:0:T=25,
+phiX174	1380	.	G	C	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:0,2:0,0.0384615384615:C=2,T=1,G=50,	0:0,0:0,0:T=1,G=32,
+phiX174	1381	.	T	G	0	.	AC=3;AF=0.0361445783133	GT:AC:AF:NC	0:3:0.0576923076923:A=1,T=49,G=3,	0:0:0:T=31,
+phiX174	1384	.	T	G	0	.	AC=4;AF=0.0512820512821	GT:AC:AF:NC	0:4:0.0816326530612:T=45,G=4,	0:0:0:T=29,G=1,
+phiX174	1385	.	T	G	0	.	AC=4;AF=0.0512820512821	GT:AC:AF:NC	0:4:0.0816326530612:T=45,G=4,	0:0:0:C=1,T=29,
+phiX174	1391	.	A	C	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:2:0.0408163265306:A=47,C=2,	0:0:0:A=30,C=1,
+phiX174	1393	.	C	A	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:2:0.0425531914894:A=2,C=45,	0:0:0:C=32,
+phiX174	1396	.	T	G	0	.	AC=3;AF=0.04	GT:AC:AF:NC	0:3:0.0697674418605:T=40,G=3,	0:0:0:T=32,
+phiX174	1397	.	A	C	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:0:0:A=42,T=1,	0:2:0.0625:A=30,C=2,
+phiX174	1400	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.047619047619:A=40,C=2,G=1,	0:0:0:A=31,
+phiX174	1401	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.047619047619:A=40,C=2,	0:0:0:A=31,
+phiX174	1434	.	C	T	0	.	AC=2;AF=0.0571428571429	GT:AC:AF:NC	0:2:0.142857142857:C=12,T=2,	0:0:0:C=21,
+phiX174	1438	.	G	C	0	.	AC=2;AF=0.0526315789474	GT:AC:AF:NC	0:0:0:G=15,	0:2:0.0869565217391:C=2,G=21,
+phiX174	1450	.	T	G	0	.	AC=3;AF=0.0789473684211	GT:AC:AF:NC	0:3:0.176470588235:A=1,C=1,T=14,G=3,	0:0:0:T=21,G=1,
+phiX174	1459	.	T	G	0	.	AC=3;AF=0.075	GT:AC:AF:NC	0:3:0.142857142857:C=1,T=18,G=3,	0:0:0:T=19,
+phiX174	1470	.	C	A	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:0:0:C=29,	0:2:0.0869565217391:A=2,C=21,
+phiX174	1484	.	C	G	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:2:0.0740740740741:A=1,C=25,G=2,	0:0:0:C=25,
+phiX174	1487	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0689655172414:T=27,G=2,	0:0:0:T=26,
+phiX174	1491	.	G	T	0	.	AC=2;AF=0.037037037037	GT:AC:AF:NC	0:0:0:T=1,G=26,	0:2:0.0714285714286:T=2,G=26,
+phiX174	1503	.	A	C	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:0:0:A=20,C=1,	0:2:0.0833333333333:A=22,C=2,
+phiX174	1536	.	A	C	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.111111111111:A=24,C=3,	0:0:0:A=28,C=1,
+phiX174	1541	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0740740740741:T=25,G=2,	0:0:0:T=28,
+phiX174	1553	.	A	G	0	.	AC=2;AF=0.0408163265306	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=1,G=2,	0:0:0:A=28,
+phiX174	1566	.	C	G	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0:0:C=30,T=1,	0:2:0.0571428571429:C=33,G=2,
+phiX174	1572	.	T	G	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:2:0.0625:T=30,G=2,	0:0:0:T=32,
+phiX174	1574	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0882352941176:C=3,G=31,	0:0:0:G=34,
+phiX174	1575	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=33,T=1,
+phiX174	1577	.	A	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:0:0:A=35,	0:3:0.0909090909091:A=30,C=3,
+phiX174	1581	.	T	G	0	.	AC=2;AF=0.0285714285714	GT:AC:AF:NC	0:2:0.0555555555556:C=1,T=34,G=2,	0:0:0:T=34,G=1,
+phiX174	1591	.	A	C	0	.	AC=3;AF=0.037037037037	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=43,C=1,
+phiX174	1597	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0606060606061:T=31,G=2,	0:0:0:C=1,T=45,
+phiX174	1603	.	T	G	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0625:T=30,G=2,	0:0:0:T=50,
+phiX174	1606	.	T	G	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0666666666667:A=1,C=1,T=28,G=2,	0:0:0:T=52,
+phiX174	1611	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0769230769231:A=24,C=2,	0:0:0:A=56,C=1,
+phiX174	1613	.	A	G	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:0:0:A=25,C=1,G=1,	0:2:0.0344827586207:A=56,G=2,
+phiX174	1618	.	C	A	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0666666666667:A=2,C=28,G=1,	0:0:0:A=1,C=57,
+phiX174	1619	.	C	A	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0666666666667:A=2,C=28,	0:0:0:C=57,
+phiX174	1621	.	A	C	0	.	AC=3;AF=0.0352941176471	GT:AC:AF:NC	0:0:0:A=31,T=1,G=1,	0:3:0.0555555555556:A=51,C=3,
+phiX174	1622	.	G	T	0	.	AC=2;AF=0.0224719101124	GT:AC:AF:NC	0:0:0:A=1,G=35,	0:2:0.037037037037:T=2,G=52,
+phiX174	1624	.	A	G	0	.	AC=2;AF=0.0235294117647	GT:AC:AF:NC	0:2:0.0555555555556:A=34,T=1,G=2,	0:0:0:A=49,
+phiX174	1627	.	T	G,C	0	.	AC=2,2;AF=0.0246913580247,0.0246913580247	GT:AC:AF:NC	0:0,0:0,0:T=37,G=1,	0:2,2:0.0454545454545,0.0454545454545:C=2,T=40,G=2,
+phiX174	1630	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0526315789474:C=1,T=36,G=2,	0:0:0:T=40,
+phiX174	1632	.	A	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=41,
+phiX174	1634	.	T	G	0	.	AC=4;AF=0.0493827160494	GT:AC:AF:NC	0:2:0.047619047619:C=1,T=40,G=2,	0:2:0.0512820512821:T=37,G=2,
+phiX174	1635	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0714285714286:T=39,G=3,	0:0:0:T=40,
+phiX174	1639	.	G	A	0	.	AC=3;AF=0.0405405405405	GT:AC:AF:NC	0:3:0.075:A=3,T=1,G=37,	0:0:0:G=34,
+phiX174	1645	.	T	G	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0425531914894:T=45,G=2,	0:0:0:T=27,G=1,
+phiX174	1647	.	A	T,G,C	0	.	AC=2,2,2;AF=0.0253164556962,0.0253164556962,0.0253164556962	GT:AC:AF:NC	0:0,2,2:0,0.0384615384615,0.0384615384615:A=48,C=2,G=2,	0:2,0,0:0.0740740740741,0,0:A=25,T=2,
+phiX174	1652	.	G	A	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.037037037037:A=2,G=52,	0:0:0:G=29,
+phiX174	1656	.	T	G	0	.	AC=3;AF=0.0348837209302	GT:AC:AF:NC	0:3:0.0555555555556:T=51,G=3,	0:0:0:T=32,G=1,
+phiX174	1658	.	A	C	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.04:A=48,C=2,	0:0:0:A=33,
+phiX174	1659	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0625:C=1,T=45,G=3,	0:0:0:T=34,
+phiX174	1663	.	T	G	0	.	AC=4;AF=0.0454545454545	GT:AC:AF:NC	0:0:0:A=1,T=53,	0:4:0.114285714286:T=31,G=4,
+phiX174	1665	.	C	A	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0384615384615:A=2,C=50,G=1,	0:0:0:C=42,
+phiX174	1670	.	G	C	0	.	AC=2;AF=0.0224719101124	GT:AC:AF:NC	0:0,2:0,0.0408163265306:A=1,C=2,T=1,G=47,	0:0,0:0,0:T=1,G=40,
+phiX174	1671	.	G	T	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0384615384615:C=1,T=2,G=50,	0:0:0:G=42,
+phiX174	1672	.	A	G	0	.	AC=3;AF=0.0315789473684	GT:AC:AF:NC	0:3:0.0545454545455:A=52,G=3,	0:0:0:A=40,T=1,
+phiX174	1673	.	G	T	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0377358490566:C=1,T=2,G=51,	0:0:0:G=41,
+phiX174	1674	.	G	T	0	.	AC=4;AF=0.04	GT:AC:AF:NC	0:4:0.0714285714286:T=4,G=52,	0:0:0:G=44,
+phiX174	1677	.	A	C	0	.	AC=3;AF=0.031914893617	GT:AC:AF:NC	0:3:0.0566037735849:A=50,C=3,N=1,	0:0:0:A=41,
+phiX174	1678	.	A	C	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:0:0:A=54,C=1,G=1,	0:2:0.05:A=38,C=2,
+phiX174	1682	.	T	C	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0408163265306:C=2,T=47,G=1,	0:0:0:C=1,T=38,
+phiX174	1685	.	T	G	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:2:0.046511627907:A=1,T=41,G=2,	0:0:0:T=41,G=1,
+phiX174	1686	.	A	C	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:0:0:A=39,C=1,T=1,G=1,	0:2:0.0512820512821:A=37,C=2,
+phiX174	1687	.	T	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.047619047619:C=2,T=40,	0:0:0:A=1,T=40,
+phiX174	1688	.	G	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.05:C=2,T=1,G=38,	0:0:0:G=41,
+phiX174	1689	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0487804878049:A=39,C=2,T=1,G=1,	0:0:0:A=41,C=1,
+phiX174	1691	.	G	C	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.05:C=2,T=1,G=38,	0:0:0:G=37,
+phiX174	1693	.	T	G	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:0:0:T=42,G=1,	0:2:0.047619047619:T=40,G=2,
+phiX174	1695	.	A	C	0	.	AC=2;AF=0.0232558139535	GT:AC:AF:NC	0:2:0.046511627907:A=41,C=2,	0:0:0:A=43,C=1,
+phiX174	1702	.	T	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.0512820512821:A=1,C=2,T=37,	0:0:0:C=1,T=42,
+phiX174	1707	.	T	C	0	.	AC=2;AF=0.0232558139535	GT:AC:AF:NC	0:2:0.05:C=2,T=38,	0:0:0:T=46,G=1,
+phiX174	1708	.	A	C	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:2:0.0526315789474:A=36,C=2,	0:0:0:A=46,C=1,G=1,
+phiX174	1710	.	T	C	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:0:0:C=1,T=34,	0:2:0.0434782608696:C=2,T=44,
+phiX174	1723	.	T	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0588235294118:C=2,T=32,	0:0:0:T=48,
+phiX174	1724	.	A	C	0	.	AC=4;AF=0.0481927710843	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:2:0.0416666666667:A=46,C=2,
+phiX174	1725	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0606060606061:A=31,C=2,	0:0:0:A=49,
+phiX174	1726	.	T	G	0	.	AC=3;AF=0.037037037037	GT:AC:AF:NC	0:3:0.0882352941176:T=31,G=3,	0:0:0:C=1,T=47,
+phiX174	1730	.	T	G	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:C=1,T=28,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1739	.	G	T	0	.	AC=3;AF=0.0434782608696	GT:AC:AF:NC	0:3:0.09375:T=3,G=29,	0:0:0:G=37,
+phiX174	1746	.	A	C	0	.	AC=2;AF=0.0338983050847	GT:AC:AF:NC	0:2:0.0689655172414:A=27,C=2,	0:0:0:A=30,C=1,G=1,
+phiX174	1749	.	T	G,C	0	.	AC=3,2;AF=0.044776119403,0.0298507462687	GT:AC:AF:NC	0:3,2:0.0857142857143,0.0571428571429:C=2,T=30,G=3,	0:0,0:0,0:T=32,G=1,
+phiX174	1755	.	G	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.05:C=2,G=38,	0:0:0:C=1,G=33,
+phiX174	1756	.	A	G,C	0	.	AC=2,2;AF=0.0281690140845,0.0281690140845	GT:AC:AF:NC	0:0,2:0,0.0526315789474:A=36,C=2,T=1,G=1,	0:2,0:0.0606060606061,0:A=31,C=1,G=2,
+phiX174	1761	.	A	G	0	.	AC=2;AF=0.0285714285714	GT:AC:AF:NC	0:2:0.0512820512821:A=37,C=1,G=2,	0:0:0:A=31,
+phiX174	1764	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=28,
+phiX174	1766	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0487804878049:A=39,C=2,	0:0:0:A=27,
+phiX174	1769	.	T	G	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:2:0.0526315789474:A=1,C=1,T=36,G=2,	0:0:0:T=26,G=1,
+phiX174	1771	.	G	C	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:2:0.0526315789474:C=2,G=36,	0:0:0:G=29,
+phiX174	1772	.	T	G	0	.	AC=4;AF=0.0597014925373	GT:AC:AF:NC	0:4:0.108108108108:C=1,T=33,G=4,	0:0:0:T=30,
+phiX174	1774	.	A	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0810810810811:A=34,G=3,	0:0:0:A=31,
+phiX174	1775	.	G	A	0	.	AC=2;AF=0.030303030303	GT:AC:AF:NC	0:2:0.0571428571429:A=2,G=33,	0:0:0:G=31,
+phiX174	1776	.	G	T	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0540540540541:T=2,G=35,	0:0:0:G=32,
+phiX174	1778	.	C	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0526315789474:C=36,G=2,	0:0:0:C=31,
+phiX174	1779	.	A	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.075:A=37,C=3,	0:0:0:A=28,
+phiX174	1781	.	T	G	0	.	AC=5;AF=0.0714285714286	GT:AC:AF:NC	0:2:0.0487804878049:C=1,T=39,G=2,	0:3:0.103448275862:T=26,G=3,
+phiX174	1782	.	T	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.075:T=37,G=3,	0:0:0:A=1,T=28,
+phiX174	1783	.	T	C	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:2:0.0512820512821:C=2,T=37,	0:0:0:T=28,G=1,
+phiX174	1788	.	G	T	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:0:0:A=1,T=1,G=29,	0:2:0.0689655172414:T=2,G=27,
+phiX174	1792	.	T	G	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:2:0.0606060606061:T=31,G=2,	0:0:0:T=25,G=1,
+phiX174	1802	.	A	C	0	.	AC=2;AF=0.030303030303	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=31,C=1,
+phiX174	1804	.	C	T	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0588235294118:A=1,C=32,T=2,	0:0:0:C=34,
+phiX174	1805	.	T	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0857142857143:T=32,G=3,	0:0:0:T=33,G=1,
+phiX174	1806	.	A	C	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=34,G=1,
+phiX174	1824	.	G	C	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0714285714286:C=2,T=1,G=26,	0:0:0:G=43,
+phiX174	1825	.	T	G	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0689655172414:T=27,G=2,	0:0:0:T=42,G=1,
+phiX174	1833	.	T	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:0:0:A=1,T=24,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1843	.	T	G	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:2:0.08:A=1,T=23,G=2,	0:0:0:T=38,
+phiX174	1847	.	A	C	0	.	AC=2;AF=0.0333333333333	GT:AC:AF:NC	0:2:0.0833333333333:A=22,C=2,T=1,G=1,	0:0:0:A=36,
+phiX174	1854	.	T	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:T=26,	0:2:0.0487804878049:T=39,G=2,
+phiX174	1860	.	T	G	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:0:0:A=1,T=20,	0:2:0.0606060606061:T=31,G=2,
+phiX174	1863	.	C	G	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=17,G=2,	0:0:0:C=33,
+phiX174	1866	.	T	G	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:0:0:T=19,G=1,	0:2:0.0645161290323:T=29,G=2,
+phiX174	1875	.	T	A	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:2:0.0952380952381:A=2,T=19,	0:0:0:T=32,G=1,
+phiX174	1876	.	T	G	0	.	AC=3;AF=0.0588235294118	GT:AC:AF:NC	0:3:0.157894736842:C=1,T=16,G=3,	0:0:0:T=32,G=1,
+phiX174	1882	.	T	G	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:2:0.105263157895:T=17,G=2,	0:0:0:T=34,
+phiX174	1889	.	A	C	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:2:0.0869565217391:A=21,C=2,	0:0:0:A=27,
+phiX174	1913	.	G	C	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:0:0:G=27,	0:2:0.0869565217391:C=2,G=21,
+phiX174	1915	.	T	G	0	.	AC=2;AF=0.0425531914894	GT:AC:AF:NC	0:0:0:A=1,T=24,	0:2:0.0869565217391:T=21,G=2,
+phiX174	1917	.	A	T	0	.	AC=2;AF=0.0444444444444	GT:AC:AF:NC	0:2,0:0.0833333333333,0:A=22,T=2,G=1,	0:0,0:0,0:A=21,G=1,
+phiX174	1930	.	T	A	0	.	AC=2;AF=0.0526315789474	GT:AC:AF:NC	0:2:0.0909090909091:A=2,T=20,	0:0:0:T=16,
+phiX174	1934	.	A	C	0	.	AC=2;AF=0.0512820512821	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=2,T=1,	0:0:0:A=18,C=1,
+phiX174	1940	.	A	C	0	.	AC=2;AF=0.046511627907	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=2,	0:0:0:A=22,C=1,
+phiX174	1942	.	T	G	0	.	AC=2;AF=0.047619047619	GT:AC:AF:NC	0:0:0:T=19,	0:2:0.0869565217391:T=21,G=2,
+phiX174	1945	.	T	G	0	.	AC=3;AF=0.0769230769231	GT:AC:AF:NC	0:0:0:T=15,G=1,	0:3:0.125:T=21,G=3,
+phiX174	1954	.	T	C	0	.	AC=2;AF=0.0625	GT:AC:AF:NC	0:0,0:0,0:T=12,G=1,	0:0,2:0,0.1:C=2,T=18,G=1,
+phiX174	1959	.	T	G	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:2:0.153846153846:T=11,G=2,	0:0:0:T=23,
+phiX174	1994	.	A	C	0	.	AC=2;AF=0.0512820512821	GT:AC:AF:NC	0:2:0.153846153846:A=11,C=2,	0:0:0:A=26,
+phiX174	1998	.	A	G	0	.	AC=2;AF=0.05	GT:AC:AF:NC	0:2:0.142857142857:A=12,G=2,	0:0:0:A=26,
diff --git a/tool_collections/vcflib/vcfcheck/test-data/vcflib-test-genome-phix.fa b/tool_collections/vcflib/vcfcheck/test-data/vcflib-test-genome-phix.fa
new file mode 100644
index 00000000000..48a6b46678a
--- /dev/null
+++ b/tool_collections/vcflib/vcfcheck/test-data/vcflib-test-genome-phix.fa
@@ -0,0 +1,2 @@
+>phiX174
+GAGTTTTATCGCTTCCATGACGCAGAAGTTAACACTTTCGGATATTTCTGATGAGTCGAAAAATTATCTTGATAAAGCAGGAATTACTACTGCTTGTTTACGAATTAAATCGAAGTGGACTGCTGGCGGAAAATGAGAAAATTCGACCTATCCTTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGTTTAGATATGAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTGGATTACTATCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAGAAATCATGAGTCAAGTTACTGAACAATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTAACCGAAGATGATTTCGATTTTCTGACGAGTAACAAAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCTTGCGTTTATGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTTTATTGCTGCCGTCATTGCTTATTATGTTCATCCCGTCAACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTACGGAAAACATTATTAATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAACACTGACGTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAAATTACGTGCaGAAGGAGTGATGTAATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATTTCAACTACTCCGGTTATCGCTGGCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTTTTTATGTCCCTCATCGTCACGTTTATGGTGAACAGTGGATTAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCCGACTGTTAACACTACTGGTTATATTGACCATGCCGCTTTTCTTGGCACGATTAACCCTGATACCAATAAAATCCCTAAGCATTTGTTTCAGGGTTATTTGAATATCTATAACAACTATTTTAAAGCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATCAAGATGATGCTCGTTATGGTTTCCGTTGCTGCCATCTCAAAAACATTTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGACTTCTACCACATCTATTGACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGCATACTGACCAAGAACGTGATTACTTCATGCAGCGTTACCgTGATGTTATTTCTTCATTTGGAGGTAAAACCTCTTATGACGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCTATGATGTTGATGGAACTGACCAAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAACATTCTGTGCCGCGTTTCTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGCGACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGCTTTGACTTATACCGATATTGCTGGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTGCTGAGGGTCAGTGGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGATTATGACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGAATAGTCAGGTTAAATTTAATGTGACCGTTTATCGCAATCTGCCGACCACTCGCGATTCAATCATGACTTCGTGATAAAAGATTGAGTGTGAGGTTATAACGCCGAAGCGGTAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACCAAGCGAAGCGCGGTAGGTTTTCTGCTTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTCGCCATAATTCAAACTTTTTTTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTTTACAGACACCTAAAGCTACATCGTCAACGTTATATTTTGATAGTTTGACGGTTAATGCTGGTAATGGTGGTTTTCTTCATTGCATTCAGATGGATACATCTGTCAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGCCGACCCTAAATTTTTTGCCTGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTCCCGACTGCCTATGATGTTTATCCTTTGAATGGTCGCCATGATGGTGGTTATTATACCGTCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTACGCCGGGCAATAAtGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGGTGATTTATGTTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAAAGCCGCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACCGATAACAATACTGTAGGCATGGGTGATGCTGGTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCAACTGGACAATCAGAAAGAGATTGCCGAGATGCAAAATGAGACTCAAAAAGAGATTGCTGGCATTCAGTCGGCGACTTCACGCCAGAATACGAAAGACCAGGTATATGCACAAAATGAGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGCGTTGCGTCTATTATGGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAAGCTCAAACGGCTGGTCAGTATTTTACCAATGACCAAATCAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCTCTTCTCATATTGGCGCTACTGCAAAGGATATTTCTAATGTCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTTTTCATGGTATTGATAAAGCTGTTGCCGATACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTCTAATTTGTCTAGGAAATAACCGTCAGGATTGACACCCTCCCAATTGTATGTTTTCATGCCTCCAAATCTTGGAGGCTTTTTTATGGTTCGTTCTTATTACCCTTCTGAATGTCACGCTGATTATTTTGACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAATGCTTGGCTTCCATAAGCAGATGGATAACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAGGGCGTTGAGTTCGATAATGGTGATATGTATGTTGACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTACTGAGAAGTTAATGGATGAATTGGCACAATGCTACAATGTGCTCCCCCAACTTGATATTAATAACACTATAGACCACCGCCCCGAAGGGGACGAAAAATGGTTTTTAGAGAACGAGAAGACGGTTACGCAGTTTTGCCGCAAGCTGGCTGCTGAACGCCCTCTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTaCTATTCAGCGTTTGATGAATGCAATGCGACAGGCTCATGCTGATGGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCTGACGACCGATTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGTGACTATTTTCGTGATATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATGATTCACACGCCGACTGCTATCAGTATTTTTGTGTGCCTGAGTATGGTACAGCTAATGGCCGTCTTCATTTCCATGCGGTGCAtTTTATGCGGACACTTCCTACAGGTAGCGTTGACCCTAATTTTGGTCGTCGGGTACGCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGTGGCCTTATGGTTACAGTATGCCCATCGCAGTTCGCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTAAATACGTTAACAAAAAGTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCACTAAAAACCAAGCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAGAATGAGCCGCAACTTCGGGATGAAAATGCTCACAATGACAAATCTGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGATATTGAAGCAGAACGCAAAAAGAGAGATGAGATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTTTGGCGGCGCAACCTGTGACGACAAATCTGCTCAAATTTATGCGCGCTTCGATAAAAATGATTGGCGTATCCAACCTGCA
diff --git a/tool_collections/vcflib/vcfcheck/tool-data/fasta_indexes.loc.sample b/tool_collections/vcflib/vcfcheck/tool-data/fasta_indexes.loc.sample
new file mode 120000
index 00000000000..6d7aec6c449
--- /dev/null
+++ b/tool_collections/vcflib/vcfcheck/tool-data/fasta_indexes.loc.sample
@@ -0,0 +1 @@
+../../tool-data/fasta_indexes.loc.sample
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfcheck/tool-data/tool_data_table_conf.xml.sample b/tool_collections/vcflib/vcfcheck/tool-data/tool_data_table_conf.xml.sample
new file mode 120000
index 00000000000..7bfcf015ca6
--- /dev/null
+++ b/tool_collections/vcflib/vcfcheck/tool-data/tool_data_table_conf.xml.sample
@@ -0,0 +1 @@
+../../tool-data/tool_data_table_conf.xml.sample
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfcheck/vcfcheck.xml b/tool_collections/vcflib/vcfcheck/vcfcheck.xml
new file mode 100644
index 00000000000..7f78e5ec741
--- /dev/null
+++ b/tool_collections/vcflib/vcfcheck/vcfcheck.xml
@@ -0,0 +1,74 @@
+<tool id="vcfcheck" name="VCFcheck:" version="@WRAPPER_VERSION@.0">
+    <description>Verify that the reference allele matches the reference genome</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"></expand>
+    <stdio>
+        <regex match="index file" source="stderr" level="warning"/>
+    </stdio>
+    <command>
+        #set $reference_fasta_filename = "localref.fa"
+        #if str( $reference_source.reference_source_selector ) == "history":
+            ln -s "${reference_source.ref_file}" "${reference_fasta_filename}" &amp;&amp;
+        #else:
+            #set $reference_fasta_filename = str( $reference_source.ref_file.fields.path )
+        #end if
+        vcfcheck $failure_selector -f "${reference_fasta_filename}" "${input_vcf}" > "${out_file1}"</command>
+    <inputs>
+        <conditional name="reference_source">
+            <param name="reference_source_selector" type="select" label="Choose the source for the reference genome">
+                <option value="cached">Locally cached</option>
+                <option value="history">History</option>
+            </param>
+            <when value="cached">
+                <param name="ref_file" type="select" label="Select reference genome">
+                    <options from_data_table="fasta_indexes">
+                    <!--<filter type="data_meta" key="dbkey" ref="input_bam" column="value"/>-->
+                    </options>
+                    <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/>
+                </param>
+                <param name="input_vcf" type="data" format="vcf" label="Select VCF dataset">
+                <!-- Validators are commented to allow users apply too to any build. May need to be revised in the future
+                <validator type="unspecified_build" />
+                <validator type="dataset_metadata_in_data_table" table_name="fasta_indexes" metadata_name="dbkey" metadata_column="1" message="Sequences are not currently available for the specified build." />
+                -->
+                </param>
+            </when>
+            <when value="history"> <!-- FIX ME!!!! -->
+                <param name="ref_file" type="data" format="fasta" label="Using reference file" />
+                <param name="input_vcf" type="data" format="vcf" label="Select VCF dataset" />
+            </when>
+        </conditional>
+        <param name="failure_selector" type="select" display="radio" label="Exclude or include failed sites">
+            <option value="-x">Exculde failures (-x option)</option>
+            <option value="-k">Keep failures (-k option)</option>
+        </param>
+    </inputs>
+    <outputs>
+        <data format="vcf" name="out_file1" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="reference_source_selector" value="history" />
+            <param name="failure_selection" value="-x" />
+            <param name="input_vcf" value="vcflib-phix.vcf"/>
+            <param name="ref_file" value="vcflib-test-genome-phix.fa" />
+            <output name="out_file1" file="vcfcheck-test1.vcf"/>
+        </test>
+    </tests>
+    <help>
+
+Verifies that the VCF REF field matches the reference as described.  
+
+The options are::
+
+    -x, --exclude-failures If a record fails, don't print it.  Otherwise do.
+    -k, --keep-failures    Print if the record fails, otherwise not.
+
+----
+
+Vcfcheck @IS_PART_OF_VCFLIB@
+    </help>
+    <expand macro="citations" />
+</tool>
diff --git a/tool_collections/vcflib/vcfcombine/.shed.yml b/tool_collections/vcflib/vcfcombine/.shed.yml
new file mode 100644
index 00000000000..f7b169b5f25
--- /dev/null
+++ b/tool_collections/vcflib/vcfcombine/.shed.yml
@@ -0,0 +1,14 @@
+categories:
+- Variant Analysis
+description: Combine multiple VCF datasets
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  Combines VCF files positionally, combining samples when sites and
+  alleles are identical. Any number of VCF files may be combined. The INFO field and
+  other columns are taken from one of the files which are combined when records in
+  multiple files match. Alleles must have identical ordering to be combined into one
+  record. If they do not, multiple records will be emitted.
+name: vcfcombine
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfcombine
+type: unrestricted
diff --git a/tool_collections/vcflib/vcfcombine/macros.xml b/tool_collections/vcflib/vcfcombine/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcfcombine/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfcombine/test-data/vcfcombine-test1.vcf b/tool_collections/vcflib/vcfcombine/test-data/vcfcombine-test1.vcf
new file mode 100644
index 00000000000..31d09fb21b5
--- /dev/null
+++ b/tool_collections/vcflib/vcfcombine/test-data/vcfcombine-test1.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	AF=0.5;DP=14;NS=3	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	AF=0.017;DP=11;NS=3	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	AA=T;AF=0.333,0.667;DP=10;NS=2	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	AA=T;DP=13;NS=3	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	AA=G;AC=3,1;AN=6;DP=9;NS=3	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	0	.	.	GT	0/0	0|0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcfcombine/test-data/vcflib.vcf b/tool_collections/vcflib/vcfcombine/test-data/vcflib.vcf
new file mode 100644
index 00000000000..e8dd794d9bd
--- /dev/null
+++ b/tool_collections/vcflib/vcfcombine/test-data/vcflib.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	NS=3;DP=14;AF=0.5;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	NS=3;DP=11;AF=0.017	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	NS=2;DP=10;AF=0.333,0.667;AA=T;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	NS=3;DP=13;AA=T	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	NS=3;DP=9;AA=G;AN=6;AC=3,1	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	.	.	.	GT	0/0	0|0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcfcombine/vcfcombine.xml b/tool_collections/vcflib/vcfcombine/vcfcombine.xml
new file mode 100644
index 00000000000..2a564eec975
--- /dev/null
+++ b/tool_collections/vcflib/vcfcombine/vcfcombine.xml
@@ -0,0 +1,39 @@
+<tool id="vcfcombine" name="VCFcombine:" version="@WRAPPER_VERSION@.0">
+    <description>Combine multiple VCF datasets</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"/>
+    <expand macro="stdio" />
+    <command>
+        vcfcombine
+
+        #for $input_vcf in $input_vcfs:
+            "${input_vcf}"
+        #end for
+
+        > "${out_file1}"
+
+    </command>
+    <inputs>
+        <param name="input_vcfs" type="data" format="vcf" label="Select VCF Datasets" min="2" multiple="True"/>
+    </inputs>
+    <outputs>
+        <data format="vcf" name="out_file1" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="input_vcfs" value="vcflib.vcf,vcflib.vcf" ftype="vcf"/>
+            <output name="out_file1" file="vcfcombine-test1.vcf"/>
+        </test>
+        </tests>
+    <help>
+
+Combines VCF files positionally, combining samples when sites and alleles are identical. Any number of VCF files may be combined. The INFO field and other columns are taken from one of the files, which are combined when records in multiple files match. Alleles must have identical ordering to be combined into one record. If they do not, multiple records will be emitted.
+
+-----
+
+Vcfcombine @IS_PART_OF_VCFLIB@
+    </help>
+    <expand macro="citations" />
+</tool>
diff --git a/tool_collections/vcflib/vcfcommonsamples/.shed.yml b/tool_collections/vcflib/vcfcommonsamples/.shed.yml
new file mode 100644
index 00000000000..f00bce1692c
--- /dev/null
+++ b/tool_collections/vcflib/vcfcommonsamples/.shed.yml
@@ -0,0 +1,10 @@
+categories:
+- Variant Analysis
+description: Output records belonging to samples commong between two datasets.
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  Outputs each record in the first file, removing samples not present in the second.
+name: vcfcommonsamples
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfcommonsamples
+type: unrestricted
diff --git a/tool_collections/vcflib/vcfcommonsamples/macros.xml b/tool_collections/vcflib/vcfcommonsamples/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcfcommonsamples/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfcommonsamples/test-data/vcfcommonsamples-test1-input2.vcf b/tool_collections/vcflib/vcfcommonsamples/test-data/vcfcommonsamples-test1-input2.vcf
new file mode 100644
index 00000000000..ebfcec99b4b
--- /dev/null
+++ b/tool_collections/vcflib/vcfcommonsamples/test-data/vcfcommonsamples-test1-input2.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	AF=0.5;DP=14;NS=3;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	AF=0.017;DP=11;NS=3	GT:GQ:DP:HQ	0|0:49:3:58,50	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	AA=T;AF=0.333,0.667;DP=10;NS=2;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	AA=T;DP=13;NS=3	GT:GQ:DP:HQ	0|0:54:.:56,60	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	AA=G;AC=3,1;AN=6;DP=9;NS=3	GT:GQ:DP	0/1:.:4	1/1:40:3
+20	1235237	.	T	.	0	.	.	GT	0/0	.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0|2
diff --git a/tool_collections/vcflib/vcfcommonsamples/test-data/vcfcommonsamples-test1.vcf b/tool_collections/vcflib/vcfcommonsamples/test-data/vcfcommonsamples-test1.vcf
new file mode 100644
index 00000000000..01f2a43aeaa
--- /dev/null
+++ b/tool_collections/vcflib/vcfcommonsamples/test-data/vcfcommonsamples-test1.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	AF=0.5;DP=14;NS=3;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	AF=0.017;DP=11;NS=3	GT:GQ:DP:HQ	0|0:49:3:58,50	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	AA=T;AF=0.333,0.667;DP=10;NS=2;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	AA=T;DP=13;NS=3	GT:GQ:DP:HQ	0|0:54:.:56,60	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	AA=G;AC=3,1;AN=6;DP=9;NS=3	GT:GQ:DP	0/1:.:4	1/1:40:3
+20	1235237	.	T	.	0	.	.	GT	0/0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0|2
diff --git a/tool_collections/vcflib/vcfcommonsamples/test-data/vcflib.vcf b/tool_collections/vcflib/vcfcommonsamples/test-data/vcflib.vcf
new file mode 100644
index 00000000000..e8dd794d9bd
--- /dev/null
+++ b/tool_collections/vcflib/vcfcommonsamples/test-data/vcflib.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	NS=3;DP=14;AF=0.5;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	NS=3;DP=11;AF=0.017	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	NS=2;DP=10;AF=0.333,0.667;AA=T;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	NS=3;DP=13;AA=T	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	NS=3;DP=9;AA=G;AN=6;AC=3,1	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	.	.	.	GT	0/0	0|0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcfcommonsamples/vcfcommonsamples.xml b/tool_collections/vcflib/vcfcommonsamples/vcfcommonsamples.xml
new file mode 100644
index 00000000000..655c5dcb61d
--- /dev/null
+++ b/tool_collections/vcflib/vcfcommonsamples/vcfcommonsamples.xml
@@ -0,0 +1,32 @@
+<tool id="vcfcommonsamples" name="VCFcommonSamples:" version="@WRAPPER_VERSION@.0">
+  <description>Output records belonging to samples common between two datasets</description>
+  <macros>
+    <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"/>
+  <expand macro="stdio" />
+  <command>vcfcommonsamples "${input1}" "${input2}" > "${out_file1}"</command>
+  <inputs>
+    <param format="vcf" name="input1" type="data" label="First dataset"/>
+    <param format="vcf" name="input2" type="data" label="Second dataset"/>
+  </inputs>
+  <outputs>
+    <data format="vcf" name="out_file1" />
+  </outputs>
+  <tests>
+    <test>
+      <param name="input1" value="vcflib.vcf"/>
+      <param name="input2" value="vcfcommonsamples-test1-input2.vcf"/>
+      <output name="out_file1" file="vcfcommonsamples-test1.vcf"/>
+    </test>
+    </tests>
+  <help>
+
+Outputs each record in the first file, removing samples not present in the second.
+
+----
+
+Vcfcommonsamples @IS_PART_OF_VCFLIB@
+</help>
+  <expand macro="citations" />
+</tool>
diff --git a/tool_collections/vcflib/vcfdistance/.shed.yml b/tool_collections/vcflib/vcfdistance/.shed.yml
new file mode 100644
index 00000000000..533bc22d874
--- /dev/null
+++ b/tool_collections/vcflib/vcfdistance/.shed.yml
@@ -0,0 +1,14 @@
+categories:
+- Variant Analysis
+description: Calculate distance to the nearest variant.
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  Adds a value to each VCF record indicating the distance to the
+  nearest variant in the file.     The dataset used as input to this tool must be
+  coordinate sorted. This can be achieved by either using the VCFsort utility or Galaxy''s
+  general purpose sort tool (in this case sort on the first and the second column
+  in ascending order).
+name: vcfdistance
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfdistance
+type: unrestricted
diff --git a/tool_collections/vcflib/vcfdistance/macros.xml b/tool_collections/vcflib/vcfdistance/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcfdistance/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfdistance/test-data/vcfdistance-test1.vcf b/tool_collections/vcflib/vcfdistance/test-data/vcfdistance-test1.vcf
new file mode 100644
index 00000000000..007aaac14be
--- /dev/null
+++ b/tool_collections/vcflib/vcfdistance/test-data/vcfdistance-test1.vcf
@@ -0,0 +1,32 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+##INFO=<ID=BasesToClosestVariant,Number=1,Type=Integer,Description="Number of bases to the closest variant in the file.">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	BasesToClosestVariant=1	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	BasesToClosestVariant=1	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	AF=0.5;BasesToClosestVariant=2960;DP=14;NS=3;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	AF=0.017;BasesToClosestVariant=2960;DP=11;NS=3	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	AA=T;AF=0.333,0.667;BasesToClosestVariant=119541;DP=10;NS=2;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	AA=T;BasesToClosestVariant=4330;DP=13;NS=3	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	AA=G;AC=3,1;AN=6;BasesToClosestVariant=670;DP=9;NS=3	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	0	.	BasesToClosestVariant=670	GT	0/0	0|0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcfdistance/test-data/vcflib.vcf b/tool_collections/vcflib/vcfdistance/test-data/vcflib.vcf
new file mode 100644
index 00000000000..e8dd794d9bd
--- /dev/null
+++ b/tool_collections/vcflib/vcfdistance/test-data/vcflib.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	NS=3;DP=14;AF=0.5;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	NS=3;DP=11;AF=0.017	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	NS=2;DP=10;AF=0.333,0.667;AA=T;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	NS=3;DP=13;AA=T	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	NS=3;DP=9;AA=G;AN=6;AC=3,1	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	.	.	.	GT	0/0	0|0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcfdistance/vcfdistance.xml b/tool_collections/vcflib/vcfdistance/vcfdistance.xml
new file mode 100644
index 00000000000..baf5b72304c
--- /dev/null
+++ b/tool_collections/vcflib/vcfdistance/vcfdistance.xml
@@ -0,0 +1,34 @@
+<tool id="vcfdistance" name="VCFdistance:" version="@WRAPPER_VERSION@.0">
+  <description>Calculate distance to the nearest variant</description>
+  <macros>
+    <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"/>
+  <expand macro="stdio" />
+  <command>cat "${input1}" | vcfdistance > "${out_file1}"</command>
+  <inputs>
+    <param format="vcf" name="input1" type="data" label="Select VCF dataset"/>
+  </inputs>
+  <outputs>
+    <data format="vcf" name="out_file1" />
+  </outputs>
+  <tests>
+    <test>
+      <param name="input1" value="vcflib.vcf"/>
+      <output name="out_file1" file="vcfdistance-test1.vcf"/>
+    </test>
+    </tests>
+  <help>
+
+Adds a value to each VCF record indicating the distance to the nearest variant in the file.
+
+.. class:: infomark
+
+The dataset used as input to this tool must be coordinate sorted. This can be achieved by either using the VCFsort utility or Galaxy's general purpose sort tool (in this case sort on the first and the second column in ascending order).
+
+----
+
+Vcfdistance @IS_PART_OF_VCFLIB@
+</help>
+  <expand macro="citations" />
+</tool>
diff --git a/tool_collections/vcflib/vcffilter/.shed.yml b/tool_collections/vcflib/vcffilter/.shed.yml
new file mode 100644
index 00000000000..07e608f54ea
--- /dev/null
+++ b/tool_collections/vcflib/vcffilter/.shed.yml
@@ -0,0 +1,11 @@
+categories:
+- Variant Analysis
+description: Tool for filtering VCF files
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  A vcflib-based tool for flexible filtering of VCF datasets on a
+  variety of tags. This is a galaxy wrapper for vcffilter utility from vcflib package.
+name: vcffilter
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcffilter
+type: unrestricted
diff --git a/tool_collections/vcflib/vcffilter/macros.xml b/tool_collections/vcflib/vcffilter/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcffilter/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcffilter/test-data/vcffilter-test1.vcf b/tool_collections/vcflib/vcffilter/test-data/vcffilter-test1.vcf
new file mode 100644
index 00000000000..fa6d831c5d6
--- /dev/null
+++ b/tool_collections/vcflib/vcffilter/test-data/vcffilter-test1.vcf
@@ -0,0 +1,26 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##filter="DP > 10"
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+20	14370	rs6054257	G	A	29	PASS	AF=0.5;DP=14;NS=3;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	AF=0.017;DP=11;NS=3	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1230237	.	T	.	47	PASS	AA=T;DP=13;NS=3	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
diff --git a/tool_collections/vcflib/vcffilter/test-data/vcflib.vcf b/tool_collections/vcflib/vcffilter/test-data/vcflib.vcf
new file mode 100644
index 00000000000..e8dd794d9bd
--- /dev/null
+++ b/tool_collections/vcflib/vcffilter/test-data/vcflib.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	NS=3;DP=14;AF=0.5;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	NS=3;DP=11;AF=0.017	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	NS=2;DP=10;AF=0.333,0.667;AA=T;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	NS=3;DP=13;AA=T	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	NS=3;DP=9;AA=G;AN=6;AC=3,1	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	.	.	.	GT	0/0	0|0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcffilter/vcffilter.xml b/tool_collections/vcflib/vcffilter/vcffilter.xml
new file mode 100644
index 00000000000..a0f19c22475
--- /dev/null
+++ b/tool_collections/vcflib/vcffilter/vcffilter.xml
@@ -0,0 +1,76 @@
+<tool id="vcffilter2" name="VCFfilter:" version="@WRAPPER_VERSION@.0">
+    <description>filter VCF data in a variety of attributes</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements">
+        <requirement type="package" version="1.3">htslib</requirement>
+    </expand>
+    <expand macro="stdio" />
+    <command>
+        <!-- This tools depends on tabix functionality, which is currently distributed with Galaxy itself via a pysam egg -->
+        ln -s "${input1}" input1.vcf &amp;&amp;
+        bgzip input1.vcf &amp;&amp;
+        tabix -p vcf input1.vcf.gz &amp;&amp;
+        vcffilter ${filterList} input1.vcf.gz  > "${out_file1}"
+    </command>
+
+    <inputs>
+        <param name="filterList" type="text" value="-f &quot;DP &gt; 10&quot;" label="Specify filterting expression" help="See explanation of filtering options below">
+            <sanitizer>
+                <valid initial="string.printable">
+                    <remove value="&apos;"/>
+                </valid>
+                <mapping initial="none">
+                    <add source="&apos;" target="__sq__"/>
+                </mapping>
+            </sanitizer>
+        </param>
+        <param format="vcf" name="input1" type="data" label="VCF dataset to filter"/>
+    </inputs>
+    <outputs>
+        <data format="vcf" name="out_file1" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="filterList" value="-f &quot;DP &gt; 10&quot;"/>
+            <param name="input1" value="vcflib.vcf"/>
+            <output name="out_file1" file="vcffilter-test1.vcf"/>
+        </test>
+    </tests>
+    <help>
+You can specify the following options within the **Specify filtering expression** box in any combination::
+
+    -f, --info-filter     specifies a filter to apply to the info fields of records, removes alleles which do not pass the filter
+    -g, --genotype-filter specifies a filter to apply to the genotype fields of records
+    -s, --filter-sites    filter entire records, not just alleles
+    -t, --tag-pass        tag vcf records as positively filtered with this tag, print all records
+    -F, --tag-fail        tag vcf records as negatively filtered with this tag, print all records
+    -A, --append-filter   append the existing filter tag, don't just replace it
+    -a, --allele-tag      apply -t on a per-allele basis.  adds or sets the corresponding INFO field tag
+    -v, --invert          inverts the filter, e.g. grep -v
+    -o, --or              use logical OR instead of AND to combine filters
+    -r, --region          specify a region on which to target the filtering (must be used in conjunction with -f or -g)
+
+Filters are specified in the form {ID} {operator} {value}::
+
+ -f "DP > 10"          # for info fields
+ -g "GT = 1|1"         # for genotype fields
+ -f "CpG"              # for 'flag' fields
+
+Any number of filters may be specified.  They are combined via logical AND unless the --or option is specified. For convenience, you can specify "QUAL" to refer to the quality of the site, even though it does not appear in the INFO fields.
+
+Operators can be any of: =, !, &lt;, &gt;, pipe, &amp;
+
+
+To restrict output to a specific location use the -r option (must be used in conjunction with -g or -f)::
+
+ -r chr20:14000-15000  # only output calls between positions 14,000 and 15,000 on chromosome 20
+ -r chrX               # only output call on chromosome X
+
+-----
+
+Vcffilter @IS_PART_OF_VCFLIB@
+    </help>
+    <expand macro="citations" />
+</tool>
diff --git a/tool_collections/vcflib/vcffixup/.shed.yml b/tool_collections/vcflib/vcffixup/.shed.yml
new file mode 100644
index 00000000000..85de4398f40
--- /dev/null
+++ b/tool_collections/vcflib/vcffixup/.shed.yml
@@ -0,0 +1,11 @@
+categories:
+- Variant Analysis
+description: 'Count the allele frequencies across alleles present in each record in the VCF file.'
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  Uses genotypes from the VCF file to correct AC (alternate allele
+  count), AF (alternate allele frequency), NS (number of called), in the VCF records.
+name: vcffixup
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcffixup
+type: unrestricted
diff --git a/tool_collections/vcflib/vcffixup/macros.xml b/tool_collections/vcflib/vcffixup/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcffixup/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcffixup/test-data/vcffixup-test1.vcf b/tool_collections/vcflib/vcffixup/test-data/vcffixup-test1.vcf
new file mode 100644
index 00000000000..8f76832065a
--- /dev/null
+++ b/tool_collections/vcflib/vcffixup/test-data/vcffixup-test1.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+##INFO=<ID=AC,Number=A,Type=Integer,Description="Total number of alternate alleles in called genotypes">
+##INFO=<ID=AF,Number=A,Type=Float,Description="Estimated allele frequency in the range (0,1]">
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of samples with data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	AC=1;AF=0.166667;AN=6;NS=3	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	AC=1;AF=0.166667;AN=6;NS=3	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	AC=3;AF=0.5;AN=6;DP=14;NS=3;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	AC=1;AF=0.166667;AN=6;DP=11;NS=3	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	AA=T;AC=2,4;AF=0.333333,0.666667;AN=6;DP=10;NS=3;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	AA=T;AC=0;AF=0;AN=6;DP=13;NS=3	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	AA=G;AC=3,1;AF=0.5,0.166667;AN=6;DP=9;NS=3	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	0	.	AC=0;AF=0;AN=4;NS=3	GT	0/0	0|0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	AC=1,1;AF=0.2,0.2;AN=5;NS=3	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcffixup/test-data/vcflib.vcf b/tool_collections/vcflib/vcffixup/test-data/vcflib.vcf
new file mode 100644
index 00000000000..e8dd794d9bd
--- /dev/null
+++ b/tool_collections/vcflib/vcffixup/test-data/vcflib.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	NS=3;DP=14;AF=0.5;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	NS=3;DP=11;AF=0.017	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	NS=2;DP=10;AF=0.333,0.667;AA=T;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	NS=3;DP=13;AA=T	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	NS=3;DP=9;AA=G;AN=6;AC=3,1	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	.	.	.	GT	0/0	0|0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcffixup/vcffixup.xml b/tool_collections/vcflib/vcffixup/vcffixup.xml
new file mode 100644
index 00000000000..754510c59b8
--- /dev/null
+++ b/tool_collections/vcflib/vcffixup/vcffixup.xml
@@ -0,0 +1,30 @@
+<tool id="vcffixup" name="VCFfixup:" version="@WRAPPER_VERSION@.0">
+  <description>Count the allele frequencies across alleles present in each record in the VCF file</description>
+  <macros>
+    <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"/>
+  <expand macro="stdio" />
+  <command>vcffixup "${input1}" > "${out_file1}"</command>
+  <inputs>
+    <param format="vcf" name="input1" type="data" label="Select VCF dataset"/>
+  </inputs>
+  <outputs>
+    <data format="vcf" name="out_file1" />
+  </outputs>
+  <tests>
+    <test>
+      <param name="input1" value="vcflib.vcf"/>
+      <output name="out_file1" file="vcffixup-test1.vcf"/>
+    </test>
+    </tests>
+  <help>
+
+Uses genotypes from the selected VCF dataset to correct AC (alternate allele count), AF (alternate allele frequency), NS (number of called), in the VCF records.
+
+----
+
+Vcffixup @IS_PART_OF_VCFLIB@
+</help>
+  <expand macro="citations" />
+</tool>
diff --git a/tool_collections/vcflib/vcfflatten/.shed.yml b/tool_collections/vcflib/vcfflatten/.shed.yml
new file mode 100644
index 00000000000..7f9e1128291
--- /dev/null
+++ b/tool_collections/vcflib/vcfflatten/.shed.yml
@@ -0,0 +1,12 @@
+categories:
+- Variant Analysis
+description: Removes multi-allelic sites by picking the most common alternate
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  Removes multi-allelic sites by picking the most common alternate.
+  Requires allele frequency specification ''AF'' and use of ''G'' and ''A'' to specify
+  the fields which vary according to the Allele or Genotype.
+name: vcfflatten
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfflatten
+type: unrestricted
diff --git a/tool_collections/vcflib/vcfflatten/macros.xml b/tool_collections/vcflib/vcfflatten/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcfflatten/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfflatten/test-data/vcfflatten-input1.vcf b/tool_collections/vcflib/vcfflatten/test-data/vcfflatten-input1.vcf
new file mode 100644
index 00000000000..ff8bfd3b023
--- /dev/null
+++ b/tool_collections/vcflib/vcfflatten/test-data/vcfflatten-input1.vcf
@@ -0,0 +1,121 @@
+##fileformat=VCFv4.1
+##fileDate=20140610
+##source=Dan
+##reference=file:///galaxy/data/phiX/sam_index/phiX.fa
+##filter="AF > .001"
+##INFO=<ID=AC,Number=A,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=AF,Number=A,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=AF,Number=.,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=NC,Number=.,Type=String,Description="Nucleotide and indel counts">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	groupA	groupB
+phiX174	1015	.	A	C	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:0,2:0,0.2:A=8,C=2,G=1,	0:0,0:0,0:A=26,G=1,
+phiX174	1016	.	A	T	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:0:0:A=10,	0:2:0.0769230769231:A=24,T=2,
+phiX174	1038	.	A	C	0	.	AC=2;AF=0.046511627907	GT:AC:AF:NC	0:2:0.153846153846:A=11,C=2,	0:0:0:A=30,
+phiX174	1045	.	T	G	0	.	AC=2;AF=0.0425531914894	GT:AC:AF:NC	0:0:0:T=15,G=1,	0:2:0.0625:T=30,G=2,
+phiX174	1048	.	C	A	0	.	AC=2;AF=0.0434782608696	GT:AC:AF:NC	0:2:0.133333333333:A=2,C=13,	0:0:0:C=31,G=1,
+phiX174	1050	.	A	C	0	.	AC=3;AF=0.0714285714286	GT:AC:AF:NC	0:0:0:A=12,	0:3:0.1:A=27,C=3,
+phiX174	1051	.	T	G	0	.	AC=2;AF=0.0487804878049	GT:AC:AF:NC	0:2:0.166666666667:C=1,T=10,G=2,	0:0:0:T=29,G=1,
+phiX174	1054	.	T	G	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:2:0.153846153846:T=11,G=2,	0:0:0:T=31,G=1,
+phiX174	1076	.	G	C	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=2,G=17,	0:0:0:G=36,
+phiX174	1080	.	G	C	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=2,T=1,G=17,	0:0:0:C=1,G=39,
+phiX174	1092	.	T	G	0	.	AC=2;AF=0.0263157894737	GT:AC:AF:NC	0:2:0.0833333333333:C=1,T=22,G=2,	0:0:0:T=52,G=1,
+phiX174	1093	.	T	G	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.08:T=23,G=2,	0:0:0:T=52,
+phiX174	1102	.	T	C	0	.	AC=6;AF=0.0779220779221	GT:AC:AF:NC	0:2:0.0869565217391:C=2,T=21,	0:4:0.0740740740741:C=4,T=50,
+phiX174	1107	.	T	G	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:0:0:T=22,G=1,	0:2:0.04:T=48,G=2,
+phiX174	1110	.	T	C	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:2:0.125:C=2,T=14,	0:0:0:C=1,T=56,G=1,
+phiX174	1121	.	T	G	0	.	AC=3;AF=0.041095890411	GT:AC:AF:NC	0:0:0:T=19,	0:3:0.0555555555556:T=51,G=3,
+phiX174	1124	.	T	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0,2:0,0.117647058824:C=2,T=15,G=1,	0:0,0:0,0:T=48,G=1,
+phiX174	1125	.	T	G	0	.	AC=3;AF=0.0454545454545	GT:AC:AF:NC	0:3:0.176470588235:T=14,G=3,	0:0:0:T=49,G=1,
+phiX174	1131	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0952380952381:T=19,G=2,	0:0:0:T=57,
+phiX174	1141	.	T	G	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0:0:T=20,G=1,	0:2:0.0338983050847:T=57,G=2,
+phiX174	1145	.	G	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.0952380952381:C=2,G=19,	0:0:0:G=52,
+phiX174	1147	.	T	C	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0952380952381:C=2,T=19,	0:0:0:T=50,
+phiX174	1149	.	T	G,C	0	.	AC=2,2;AF=0.027027027027,0.027027027027	GT:AC:AF:NC	0:2,2:0.1,0.1:C=2,T=16,G=2,	0:0,0:0,0:T=54,
+phiX174	1156	.	T	G	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:0:0:C=1,T=15,	0:2:0.0416666666667:T=46,G=2,
+phiX174	1159	.	T	C,G	0	.	AC=2,2;AF=0.0298507462687,0.0298507462687	GT:AC:AF:NC	0:2,0:0.111111111111,0:C=2,T=16,	0:0,2:0,0.0408163265306:C=1,T=47,G=2,
+phiX174	1164	.	T	G	0	.	AC=4;AF=0.0666666666667	GT:AC:AF:NC	0:0:0:C=1,T=16,G=1,	0:4:0.0909090909091:T=40,G=4,
+phiX174	1168	.	T	G	0	.	AC=4;AF=0.0666666666667	GT:AC:AF:NC	0:2:0.0952380952381:C=1,T=19,G=2,	0:2:0.0512820512821:T=37,G=2,
+phiX174	1171	.	T	G	0	.	AC=2;AF=0.0327868852459	GT:AC:AF:NC	0:2:0.0869565217391:T=21,G=2,	0:0:0:T=38,G=1,
+phiX174	1174	.	C	A	0	.	AC=2;AF=0.0327868852459	GT:AC:AF:NC	0:2:0.0869565217391:A=2,C=21,	0:0:0:A=1,C=38,
+phiX174	1178	.	G	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:2:0.0740740740741:C=2,T=1,G=25,	0:0:0:G=38,
+phiX174	1187	.	T	G	0	.	AC=3;AF=0.04	GT:AC:AF:NC	0:3:0.0882352941176:C=1,T=31,G=3,	0:0:0:T=41,
+phiX174	1189	.	T	C	0	.	AC=3;AF=0.0379746835443	GT:AC:AF:NC	0:3:0.0857142857143:C=3,T=32,G=1,	0:0:0:T=44,
+phiX174	1200	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0882352941176:T=31,G=3,	0:0:0:T=48,G=1,
+phiX174	1202	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:0:0:T=32,	0:3:0.06:T=47,G=3,
+phiX174	1205	.	A	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.0588235294118:A=32,C=2,	0:0:0:A=47,C=1,G=1,
+phiX174	1207	.	T	G	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:A=1,T=34,	0:2:0.0425531914894:T=45,G=2,
+phiX174	1214	.	G	A	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:T=1,G=34,	0:2:0.0425531914894:A=2,G=45,
+phiX174	1216	.	C	A	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:C=34,	0:2:0.0425531914894:A=2,C=45,
+phiX174	1217	.	C	A	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.0588235294118:A=2,C=32,	0:0:0:C=46,
+phiX174	1219	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:0:0:C=1,T=33,G=1,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1223	.	C	T	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.0555555555556:C=34,T=2,	0:0:0:C=44,
+phiX174	1227	.	A	C	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0588235294118:A=32,C=2,	0:0:0:A=40,
+phiX174	1232	.	T	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:T=34,	0:2:0.0606060606061:C=1,T=31,G=2,
+phiX174	1236	.	G	T	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0606060606061:A=1,T=2,G=31,	0:0:0:G=35,
+phiX174	1237	.	T	G	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=1,T=33,G=2,	0:0:0:T=33,G=1,
+phiX174	1240	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:0,0:0,0:A=32,T=1,	0:0,2:0,0.0555555555556:A=34,C=2,T=1,
+phiX174	1245	.	G	T	0	.	AC=4;AF=0.0625	GT:AC:AF:NC	0:2:0.0666666666667:C=1,T=2,G=28,	0:2:0.0588235294118:T=2,G=32,
+phiX174	1247	.	A	T	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:2:0.0714285714286:A=26,C=1,T=2,G=1,	0:0:0:A=35,
+phiX174	1250	.	A	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:2:0.0769230769231:A=24,C=2,	0:0:0:A=39,
+phiX174	1251	.	A	G	0	.	AC=3;AF=0.044776119403	GT:AC:AF:NC	0:0:0:A=29,	0:3:0.0789473684211:A=35,C=1,G=3,
+phiX174	1253	.	T	G	0	.	AC=4;AF=0.0526315789474	GT:AC:AF:NC	0:2:0.0645161290323:T=29,G=2,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1257	.	T	G	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:0:0:T=26,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1259	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:A=28,T=1,	0:2:0.0444444444444:A=43,C=2,G=1,
+phiX174	1274	.	G	C	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.0512820512821:C=2,G=37,	0:0:0:G=44,
+phiX174	1277	.	A	C	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=40,G=1,
+phiX174	1279	.	T	G	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0:0:T=39,G=1,	0:2:0.05:C=1,T=38,G=2,
+phiX174	1284	.	T	G,C	0	.	AC=3,2;AF=0.038961038961,0.025974025974	GT:AC:AF:NC	0:3,2:0.075,0.05:C=2,T=35,G=3,	0:0,0:0,0:A=1,T=37,
+phiX174	1289	.	A	C	0	.	AC=3;AF=0.0454545454545	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=28,G=1,
+phiX174	1296	.	A	C	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:0:0:A=38,	0:2:0.0769230769231:A=24,C=2,
+phiX174	1298	.	A	C	0	.	AC=3;AF=0.0491803278689	GT:AC:AF:NC	0:3,0:0.0810810810811,0:A=34,C=3,G=1,	0:0,0:0,0:A=24,C=1,T=1,G=1,
+phiX174	1306	.	T	G	0	.	AC=5;AF=0.0943396226415	GT:AC:AF:NC	0:5:0.178571428571:T=23,G=5,	0:0:0:C=1,T=25,
+phiX174	1307	.	T	G	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.107142857143:T=25,G=3,	0:0:0:C=1,T=27,
+phiX174	1314	.	A	G	0	.	AC=5;AF=0.0877192982456	GT:AC:AF:NC	0:2,0:0.0740740740741,0:A=25,C=1,G=2,	0:3,0:0.1,0:A=27,C=1,G=3,
+phiX174	1317	.	A	C	0	.	AC=2;AF=0.0322580645161	GT:AC:AF:NC	0:2:0.0740740740741:A=25,C=2,	0:0:0:A=35,
+phiX174	1320	.	C	G	0	.	AC=2;AF=0.0322580645161	GT:AC:AF:NC	0:2:0.0769230769231:C=24,G=2,	0:0:0:C=36,
+phiX174	1322	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.107142857143:C=3,G=25,	0:0:0:G=40,
+phiX174	1323	.	C	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:C=28,	0:2:0.0512820512821:C=37,T=1,G=2,
+phiX174	1329	.	T	C	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:2:0.0666666666667:C=2,T=28,	0:0:0:T=42,G=1,
+phiX174	1335	.	C	A	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.0625:A=2,C=30,	0:0:0:C=45,
+phiX174	1338	.	T	A	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:0:0:T=32,	0:2:0.047619047619:A=2,T=40,
+phiX174	1345	.	T	C	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0,0:0,0:T=36,G=1,	0:0,2:0,0.046511627907:C=2,T=41,G=1,
+phiX174	1347	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0512820512821:A=37,C=2,G=1,	0:0:0:A=43,
+phiX174	1348	.	T	G	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.05:T=38,G=2,	0:0:0:T=43,
+phiX174	1352	.	A	T	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:A=34,G=1,	0:2:0.0512820512821:A=37,T=2,
+phiX174	1354	.	T	G,A	0	.	AC=2,2;AF=0.027027027027,0.027027027027	GT:AC:AF:NC	0:2,0:0.0526315789474,0:T=36,G=2,	0:0,2:0,0.0555555555556:A=2,T=34,G=1,
+phiX174	1359	.	T	G	0	.	AC=3;AF=0.0405405405405	GT:AC:AF:NC	0:3:0.0714285714286:T=39,G=3,	0:0:0:T=32,G=1,
+phiX174	1366	.	G	C	0	.	AC=3;AF=0.0428571428571	GT:AC:AF:NC	0:3:0.0714285714286:C=3,T=1,G=39,	0:0:0:G=28,
+phiX174	1370	.	T	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:0:0:C=1,T=45,G=1,	0:2:0.0833333333333:A=1,T=22,G=2,
+phiX174	1373	.	T	G	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0408163265306:T=47,G=2,	0:0:0:T=25,
+phiX174	1380	.	G	C	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:0,2:0,0.0384615384615:C=2,T=1,G=50,	0:0,0:0,0:T=1,G=32,
+phiX174	1381	.	T	G	0	.	AC=3;AF=0.0361445783133	GT:AC:AF:NC	0:3:0.0576923076923:A=1,T=49,G=3,	0:0:0:T=31,
+phiX174	1384	.	T	G	0	.	AC=4;AF=0.0512820512821	GT:AC:AF:NC	0:4:0.0816326530612:T=45,G=4,	0:0:0:T=29,G=1,
+phiX174	1385	.	T	G	0	.	AC=4;AF=0.0512820512821	GT:AC:AF:NC	0:4:0.0816326530612:T=45,G=4,	0:0:0:C=1,T=29,
+phiX174	1391	.	A	C	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:2:0.0408163265306:A=47,C=2,	0:0:0:A=30,C=1,
+phiX174	1393	.	C	A	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:2:0.0425531914894:A=2,C=45,	0:0:0:C=32,
+phiX174	1396	.	T	G	0	.	AC=3;AF=0.04	GT:AC:AF:NC	0:3:0.0697674418605:T=40,G=3,	0:0:0:T=32,
+phiX174	1397	.	A	C	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:0:0:A=42,T=1,	0:2:0.0625:A=30,C=2,
+phiX174	1400	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.047619047619:A=40,C=2,G=1,	0:0:0:A=31,
+phiX174	1401	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.047619047619:A=40,C=2,	0:0:0:A=31,
+phiX174	1434	.	C	T	0	.	AC=2;AF=0.0571428571429	GT:AC:AF:NC	0:2:0.142857142857:C=12,T=2,	0:0:0:C=21,
+phiX174	1438	.	G	C	0	.	AC=2;AF=0.0526315789474	GT:AC:AF:NC	0:0:0:G=15,	0:2:0.0869565217391:C=2,G=21,
+phiX174	1450	.	T	G	0	.	AC=3;AF=0.0789473684211	GT:AC:AF:NC	0:3:0.176470588235:A=1,C=1,T=14,G=3,	0:0:0:T=21,G=1,
+phiX174	1459	.	T	G	0	.	AC=3;AF=0.075	GT:AC:AF:NC	0:3:0.142857142857:C=1,T=18,G=3,	0:0:0:T=19,
+phiX174	1470	.	C	A	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:0:0:C=29,	0:2:0.0869565217391:A=2,C=21,
+phiX174	1484	.	C	G	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:2:0.0740740740741:A=1,C=25,G=2,	0:0:0:C=25,
+phiX174	1487	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0689655172414:T=27,G=2,	0:0:0:T=26,
+phiX174	1491	.	G	T	0	.	AC=2;AF=0.037037037037	GT:AC:AF:NC	0:0:0:T=1,G=26,	0:2:0.0714285714286:T=2,G=26,
+phiX174	1503	.	A	C	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:0:0:A=20,C=1,	0:2:0.0833333333333:A=22,C=2,
+phiX174	1536	.	A	C	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.111111111111:A=24,C=3,	0:0:0:A=28,C=1,
+phiX174	1541	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0740740740741:T=25,G=2,	0:0:0:T=28,
+phiX174	1553	.	A	G	0	.	AC=2;AF=0.0408163265306	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=1,G=2,	0:0:0:A=28,
+phiX174	1566	.	C	G	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0:0:C=30,T=1,	0:2:0.0571428571429:C=33,G=2,
+phiX174	1572	.	T	G	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:2:0.0625:T=30,G=2,	0:0:0:T=32,
+phiX174	1574	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0882352941176:C=3,G=31,	0:0:0:G=34,
+phiX174	1575	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=33,T=1,
+phiX174	1577	.	A	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:0:0:A=35,	0:3:0.0909090909091:A=30,C=3,
+phiX174	1581	.	T	G	0	.	AC=2;AF=0.0285714285714	GT:AC:AF:NC	0:2:0.0555555555556:C=1,T=34,G=2,	0:0:0:T=34,G=1,
+phiX174	1591	.	A	C	0	.	AC=3;AF=0.037037037037	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=43,C=1,
+phiX174	1597	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0606060606061:T=31,G=2,	0:0:0:C=1,T=45,
diff --git a/tool_collections/vcflib/vcfflatten/test-data/vcfflatten-test1.vcf b/tool_collections/vcflib/vcfflatten/test-data/vcfflatten-test1.vcf
new file mode 100644
index 00000000000..8b4fdcbc451
--- /dev/null
+++ b/tool_collections/vcflib/vcfflatten/test-data/vcfflatten-test1.vcf
@@ -0,0 +1,121 @@
+##fileformat=VCFv4.1
+##fileDate=20140610
+##source=Dan
+##reference=file:///galaxy/data/phiX/sam_index/phiX.fa
+##filter="AF > .001"
+##INFO=<ID=AC,Number=A,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=AF,Number=A,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=AF,Number=.,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=NC,Number=.,Type=String,Description="Nucleotide and indel counts">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	groupA	groupB
+phiX174	1015	.	A	C	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:0,2:0,0.2:A=8,C=2,G=1,	0:0,0:0,0:A=26,G=1,
+phiX174	1016	.	A	T	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:0:0:A=10,	0:2:0.0769230769231:A=24,T=2,
+phiX174	1038	.	A	C	0	.	AC=2;AF=0.046511627907	GT:AC:AF:NC	0:2:0.153846153846:A=11,C=2,	0:0:0:A=30,
+phiX174	1045	.	T	G	0	.	AC=2;AF=0.0425531914894	GT:AC:AF:NC	0:0:0:T=15,G=1,	0:2:0.0625:T=30,G=2,
+phiX174	1048	.	C	A	0	.	AC=2;AF=0.0434782608696	GT:AC:AF:NC	0:2:0.133333333333:A=2,C=13,	0:0:0:C=31,G=1,
+phiX174	1050	.	A	C	0	.	AC=3;AF=0.0714285714286	GT:AC:AF:NC	0:0:0:A=12,	0:3:0.1:A=27,C=3,
+phiX174	1051	.	T	G	0	.	AC=2;AF=0.0487804878049	GT:AC:AF:NC	0:2:0.166666666667:C=1,T=10,G=2,	0:0:0:T=29,G=1,
+phiX174	1054	.	T	G	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:2:0.153846153846:T=11,G=2,	0:0:0:T=31,G=1,
+phiX174	1076	.	G	C	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=2,G=17,	0:0:0:G=36,
+phiX174	1080	.	G	C	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=2,T=1,G=17,	0:0:0:C=1,G=39,
+phiX174	1092	.	T	G	0	.	AC=2;AF=0.0263157894737	GT:AC:AF:NC	0:2:0.0833333333333:C=1,T=22,G=2,	0:0:0:T=52,G=1,
+phiX174	1093	.	T	G	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.08:T=23,G=2,	0:0:0:T=52,
+phiX174	1102	.	T	C	0	.	AC=6;AF=0.0779220779221	GT:AC:AF:NC	0:2:0.0869565217391:C=2,T=21,	0:4:0.0740740740741:C=4,T=50,
+phiX174	1107	.	T	G	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:0:0:T=22,G=1,	0:2:0.04:T=48,G=2,
+phiX174	1110	.	T	C	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:2:0.125:C=2,T=14,	0:0:0:C=1,T=56,G=1,
+phiX174	1121	.	T	G	0	.	AC=3;AF=0.041095890411	GT:AC:AF:NC	0:0:0:T=19,	0:3:0.0555555555556:T=51,G=3,
+phiX174	1124	.	T	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0,2:0,0.117647058824:C=2,T=15,G=1,	0:0,0:0,0:T=48,G=1,
+phiX174	1125	.	T	G	0	.	AC=3;AF=0.0454545454545	GT:AC:AF:NC	0:3:0.176470588235:T=14,G=3,	0:0:0:T=49,G=1,
+phiX174	1131	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0952380952381:T=19,G=2,	0:0:0:T=57,
+phiX174	1141	.	T	G	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0:0:T=20,G=1,	0:2:0.0338983050847:T=57,G=2,
+phiX174	1145	.	G	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.0952380952381:C=2,G=19,	0:0:0:G=52,
+phiX174	1147	.	T	C	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0952380952381:C=2,T=19,	0:0:0:T=50,
+phiX174	1149	.	T	C	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2,2:0.1,0.1:C=2,T=16,G=2,	0:0,0:0,0:T=54,
+phiX174	1156	.	T	G	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:0:0:C=1,T=15,	0:2:0.0416666666667:T=46,G=2,
+phiX174	1159	.	T	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:2,0:0.111111111111,0:C=2,T=16,	0:0,2:0,0.0408163265306:C=1,T=47,G=2,
+phiX174	1164	.	T	G	0	.	AC=4;AF=0.0666666666667	GT:AC:AF:NC	0:0:0:C=1,T=16,G=1,	0:4:0.0909090909091:T=40,G=4,
+phiX174	1168	.	T	G	0	.	AC=4;AF=0.0666666666667	GT:AC:AF:NC	0:2:0.0952380952381:C=1,T=19,G=2,	0:2:0.0512820512821:T=37,G=2,
+phiX174	1171	.	T	G	0	.	AC=2;AF=0.0327868852459	GT:AC:AF:NC	0:2:0.0869565217391:T=21,G=2,	0:0:0:T=38,G=1,
+phiX174	1174	.	C	A	0	.	AC=2;AF=0.0327868852459	GT:AC:AF:NC	0:2:0.0869565217391:A=2,C=21,	0:0:0:A=1,C=38,
+phiX174	1178	.	G	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:2:0.0740740740741:C=2,T=1,G=25,	0:0:0:G=38,
+phiX174	1187	.	T	G	0	.	AC=3;AF=0.04	GT:AC:AF:NC	0:3:0.0882352941176:C=1,T=31,G=3,	0:0:0:T=41,
+phiX174	1189	.	T	C	0	.	AC=3;AF=0.0379746835443	GT:AC:AF:NC	0:3:0.0857142857143:C=3,T=32,G=1,	0:0:0:T=44,
+phiX174	1200	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0882352941176:T=31,G=3,	0:0:0:T=48,G=1,
+phiX174	1202	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:0:0:T=32,	0:3:0.06:T=47,G=3,
+phiX174	1205	.	A	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.0588235294118:A=32,C=2,	0:0:0:A=47,C=1,G=1,
+phiX174	1207	.	T	G	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:A=1,T=34,	0:2:0.0425531914894:T=45,G=2,
+phiX174	1214	.	G	A	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:T=1,G=34,	0:2:0.0425531914894:A=2,G=45,
+phiX174	1216	.	C	A	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:C=34,	0:2:0.0425531914894:A=2,C=45,
+phiX174	1217	.	C	A	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.0588235294118:A=2,C=32,	0:0:0:C=46,
+phiX174	1219	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:0:0:C=1,T=33,G=1,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1223	.	C	T	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.0555555555556:C=34,T=2,	0:0:0:C=44,
+phiX174	1227	.	A	C	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0588235294118:A=32,C=2,	0:0:0:A=40,
+phiX174	1232	.	T	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:T=34,	0:2:0.0606060606061:C=1,T=31,G=2,
+phiX174	1236	.	G	T	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0606060606061:A=1,T=2,G=31,	0:0:0:G=35,
+phiX174	1237	.	T	G	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=1,T=33,G=2,	0:0:0:T=33,G=1,
+phiX174	1240	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:0,0:0,0:A=32,T=1,	0:0,2:0,0.0555555555556:A=34,C=2,T=1,
+phiX174	1245	.	G	T	0	.	AC=4;AF=0.0625	GT:AC:AF:NC	0:2:0.0666666666667:C=1,T=2,G=28,	0:2:0.0588235294118:T=2,G=32,
+phiX174	1247	.	A	T	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:2:0.0714285714286:A=26,C=1,T=2,G=1,	0:0:0:A=35,
+phiX174	1250	.	A	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:2:0.0769230769231:A=24,C=2,	0:0:0:A=39,
+phiX174	1251	.	A	G	0	.	AC=3;AF=0.044776119403	GT:AC:AF:NC	0:0:0:A=29,	0:3:0.0789473684211:A=35,C=1,G=3,
+phiX174	1253	.	T	G	0	.	AC=4;AF=0.0526315789474	GT:AC:AF:NC	0:2:0.0645161290323:T=29,G=2,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1257	.	T	G	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:0:0:T=26,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1259	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:A=28,T=1,	0:2:0.0444444444444:A=43,C=2,G=1,
+phiX174	1274	.	G	C	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.0512820512821:C=2,G=37,	0:0:0:G=44,
+phiX174	1277	.	A	C	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=40,G=1,
+phiX174	1279	.	T	G	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0:0:T=39,G=1,	0:2:0.05:C=1,T=38,G=2,
+phiX174	1284	.	T	G	0	.	AC=3;AF=0.038961038961	GT:AC:AF:NC	0:3,2:0.075,0.05:C=2,T=35,G=3,	0:0,0:0,0:A=1,T=37,
+phiX174	1289	.	A	C	0	.	AC=3;AF=0.0454545454545	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=28,G=1,
+phiX174	1296	.	A	C	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:0:0:A=38,	0:2:0.0769230769231:A=24,C=2,
+phiX174	1298	.	A	C	0	.	AC=3;AF=0.0491803278689	GT:AC:AF:NC	0:3,0:0.0810810810811,0:A=34,C=3,G=1,	0:0,0:0,0:A=24,C=1,T=1,G=1,
+phiX174	1306	.	T	G	0	.	AC=5;AF=0.0943396226415	GT:AC:AF:NC	0:5:0.178571428571:T=23,G=5,	0:0:0:C=1,T=25,
+phiX174	1307	.	T	G	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.107142857143:T=25,G=3,	0:0:0:C=1,T=27,
+phiX174	1314	.	A	G	0	.	AC=5;AF=0.0877192982456	GT:AC:AF:NC	0:2,0:0.0740740740741,0:A=25,C=1,G=2,	0:3,0:0.1,0:A=27,C=1,G=3,
+phiX174	1317	.	A	C	0	.	AC=2;AF=0.0322580645161	GT:AC:AF:NC	0:2:0.0740740740741:A=25,C=2,	0:0:0:A=35,
+phiX174	1320	.	C	G	0	.	AC=2;AF=0.0322580645161	GT:AC:AF:NC	0:2:0.0769230769231:C=24,G=2,	0:0:0:C=36,
+phiX174	1322	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.107142857143:C=3,G=25,	0:0:0:G=40,
+phiX174	1323	.	C	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:C=28,	0:2:0.0512820512821:C=37,T=1,G=2,
+phiX174	1329	.	T	C	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:2:0.0666666666667:C=2,T=28,	0:0:0:T=42,G=1,
+phiX174	1335	.	C	A	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.0625:A=2,C=30,	0:0:0:C=45,
+phiX174	1338	.	T	A	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:0:0:T=32,	0:2:0.047619047619:A=2,T=40,
+phiX174	1345	.	T	C	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0,0:0,0:T=36,G=1,	0:0,2:0,0.046511627907:C=2,T=41,G=1,
+phiX174	1347	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0512820512821:A=37,C=2,G=1,	0:0:0:A=43,
+phiX174	1348	.	T	G	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.05:T=38,G=2,	0:0:0:T=43,
+phiX174	1352	.	A	T	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:A=34,G=1,	0:2:0.0512820512821:A=37,T=2,
+phiX174	1354	.	T	A	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2,0:0.0526315789474,0:T=36,G=2,	0:0,2:0,0.0555555555556:A=2,T=34,G=1,
+phiX174	1359	.	T	G	0	.	AC=3;AF=0.0405405405405	GT:AC:AF:NC	0:3:0.0714285714286:T=39,G=3,	0:0:0:T=32,G=1,
+phiX174	1366	.	G	C	0	.	AC=3;AF=0.0428571428571	GT:AC:AF:NC	0:3:0.0714285714286:C=3,T=1,G=39,	0:0:0:G=28,
+phiX174	1370	.	T	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:0:0:C=1,T=45,G=1,	0:2:0.0833333333333:A=1,T=22,G=2,
+phiX174	1373	.	T	G	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0408163265306:T=47,G=2,	0:0:0:T=25,
+phiX174	1380	.	G	C	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:0,2:0,0.0384615384615:C=2,T=1,G=50,	0:0,0:0,0:T=1,G=32,
+phiX174	1381	.	T	G	0	.	AC=3;AF=0.0361445783133	GT:AC:AF:NC	0:3:0.0576923076923:A=1,T=49,G=3,	0:0:0:T=31,
+phiX174	1384	.	T	G	0	.	AC=4;AF=0.0512820512821	GT:AC:AF:NC	0:4:0.0816326530612:T=45,G=4,	0:0:0:T=29,G=1,
+phiX174	1385	.	T	G	0	.	AC=4;AF=0.0512820512821	GT:AC:AF:NC	0:4:0.0816326530612:T=45,G=4,	0:0:0:C=1,T=29,
+phiX174	1391	.	A	C	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:2:0.0408163265306:A=47,C=2,	0:0:0:A=30,C=1,
+phiX174	1393	.	C	A	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:2:0.0425531914894:A=2,C=45,	0:0:0:C=32,
+phiX174	1396	.	T	G	0	.	AC=3;AF=0.04	GT:AC:AF:NC	0:3:0.0697674418605:T=40,G=3,	0:0:0:T=32,
+phiX174	1397	.	A	C	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:0:0:A=42,T=1,	0:2:0.0625:A=30,C=2,
+phiX174	1400	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.047619047619:A=40,C=2,G=1,	0:0:0:A=31,
+phiX174	1401	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.047619047619:A=40,C=2,	0:0:0:A=31,
+phiX174	1434	.	C	T	0	.	AC=2;AF=0.0571428571429	GT:AC:AF:NC	0:2:0.142857142857:C=12,T=2,	0:0:0:C=21,
+phiX174	1438	.	G	C	0	.	AC=2;AF=0.0526315789474	GT:AC:AF:NC	0:0:0:G=15,	0:2:0.0869565217391:C=2,G=21,
+phiX174	1450	.	T	G	0	.	AC=3;AF=0.0789473684211	GT:AC:AF:NC	0:3:0.176470588235:A=1,C=1,T=14,G=3,	0:0:0:T=21,G=1,
+phiX174	1459	.	T	G	0	.	AC=3;AF=0.075	GT:AC:AF:NC	0:3:0.142857142857:C=1,T=18,G=3,	0:0:0:T=19,
+phiX174	1470	.	C	A	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:0:0:C=29,	0:2:0.0869565217391:A=2,C=21,
+phiX174	1484	.	C	G	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:2:0.0740740740741:A=1,C=25,G=2,	0:0:0:C=25,
+phiX174	1487	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0689655172414:T=27,G=2,	0:0:0:T=26,
+phiX174	1491	.	G	T	0	.	AC=2;AF=0.037037037037	GT:AC:AF:NC	0:0:0:T=1,G=26,	0:2:0.0714285714286:T=2,G=26,
+phiX174	1503	.	A	C	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:0:0:A=20,C=1,	0:2:0.0833333333333:A=22,C=2,
+phiX174	1536	.	A	C	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.111111111111:A=24,C=3,	0:0:0:A=28,C=1,
+phiX174	1541	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0740740740741:T=25,G=2,	0:0:0:T=28,
+phiX174	1553	.	A	G	0	.	AC=2;AF=0.0408163265306	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=1,G=2,	0:0:0:A=28,
+phiX174	1566	.	C	G	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0:0:C=30,T=1,	0:2:0.0571428571429:C=33,G=2,
+phiX174	1572	.	T	G	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:2:0.0625:T=30,G=2,	0:0:0:T=32,
+phiX174	1574	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0882352941176:C=3,G=31,	0:0:0:G=34,
+phiX174	1575	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=33,T=1,
+phiX174	1577	.	A	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:0:0:A=35,	0:3:0.0909090909091:A=30,C=3,
+phiX174	1581	.	T	G	0	.	AC=2;AF=0.0285714285714	GT:AC:AF:NC	0:2:0.0555555555556:C=1,T=34,G=2,	0:0:0:T=34,G=1,
+phiX174	1591	.	A	C	0	.	AC=3;AF=0.037037037037	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=43,C=1,
+phiX174	1597	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0606060606061:T=31,G=2,	0:0:0:C=1,T=45,
diff --git a/tool_collections/vcflib/vcfflatten/vcfflatten.xml b/tool_collections/vcflib/vcfflatten/vcfflatten.xml
new file mode 100644
index 00000000000..4ac8d6d6548
--- /dev/null
+++ b/tool_collections/vcflib/vcfflatten/vcfflatten.xml
@@ -0,0 +1,30 @@
+<tool id="vcfflatten2" name="VCFflatten:" version="@WRAPPER_VERSION@.0">
+  <description>Removes multi-allelic sites by picking the most common alternate</description>
+  <macros>
+    <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"/>
+  <expand macro="stdio" />
+  <command>vcfflatten "${input1}" > "${out_file1}"</command>
+  <inputs>
+    <param format="vcf" name="input1" type="data" label="Select VCF dataset"/>
+  </inputs>
+  <outputs>
+    <data format="vcf" name="out_file1" />
+  </outputs>
+  <tests>
+    <test>
+      <param name="input1" value="vcfflatten-input1.vcf"/>
+      <output name="out_file1" file="vcfflatten-test1.vcf"/>
+    </test>
+    </tests>
+  <help>
+
+Removes multi-allelic sites by picking the most common alternate.  Requires allele frequency specification 'AF' and use of 'G' and 'A' to specify the fields which vary according to the Allele or Genotype.
+
+----
+
+Vcfflatten @IS_PART_OF_VCFLIB@
+</help>
+  <expand macro="citations" />
+</tool>
diff --git a/tool_collections/vcflib/vcfgeno2haplo/.shed.yml b/tool_collections/vcflib/vcfgeno2haplo/.shed.yml
new file mode 100644
index 00000000000..f996dd20e7a
--- /dev/null
+++ b/tool_collections/vcflib/vcfgeno2haplo/.shed.yml
@@ -0,0 +1,12 @@
+categories:
+- Variant Analysis
+description: Convert genotype-based phased alleles into haplotype alleles
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  Convert genotype-based phased alleles within a window size specified
+  by -w option into haplotype alleles. Will break haplotype construction when encountering
+  non-phased genotypes on input.
+name: vcfgeno2haplo
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfgeno2haplo
+type: unrestricted
diff --git a/tool_collections/vcflib/vcfgeno2haplo/macros.xml b/tool_collections/vcflib/vcfgeno2haplo/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcfgeno2haplo/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfgeno2haplo/test-data/vcfgeno2haplo-test1.vcf b/tool_collections/vcflib/vcfgeno2haplo/test-data/vcfgeno2haplo-test1.vcf
new file mode 100644
index 00000000000..64e79fd753d
--- /dev/null
+++ b/tool_collections/vcflib/vcfgeno2haplo/test-data/vcfgeno2haplo-test1.vcf
@@ -0,0 +1,12 @@
+##fileformat=VCFv4.1
+##fileDate=20140610
+##source=Dan
+##reference=file:///galaxy/data/phiX/sam_index/phiX.fa
+##filter="AF > .001"
+##INFO=<ID=AC,Number=A,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=AF,Number=A,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=AF,Number=.,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=NC,Number=.,Type=String,Description="Nucleotide and indel counts">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	groupA	groupB
diff --git a/tool_collections/vcflib/vcfgeno2haplo/test-data/vcflib-phix.vcf b/tool_collections/vcflib/vcfgeno2haplo/test-data/vcflib-phix.vcf
new file mode 100644
index 00000000000..362e6be2088
--- /dev/null
+++ b/tool_collections/vcflib/vcfgeno2haplo/test-data/vcflib-phix.vcf
@@ -0,0 +1,219 @@
+##fileformat=VCFv4.1
+##fileDate=20140610
+##source=Dan
+##reference=file:///galaxy/data/phiX/sam_index/phiX.fa
+##filter="AF > .001"
+##INFO=<ID=AC,Number=A,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=AF,Number=A,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=AF,Number=.,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=NC,Number=.,Type=String,Description="Nucleotide and indel counts">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	groupA	groupB
+phiX174	1015	.	A	C	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:0,2:0,0.2:A=8,C=2,G=1,	0:0,0:0,0:A=26,G=1,
+phiX174	1016	.	A	T	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:0:0:A=10,	0:2:0.0769230769231:A=24,T=2,
+phiX174	1038	.	A	C	0	.	AC=2;AF=0.046511627907	GT:AC:AF:NC	0:2:0.153846153846:A=11,C=2,	0:0:0:A=30,
+phiX174	1045	.	T	G	0	.	AC=2;AF=0.0425531914894	GT:AC:AF:NC	0:0:0:T=15,G=1,	0:2:0.0625:T=30,G=2,
+phiX174	1048	.	C	A	0	.	AC=2;AF=0.0434782608696	GT:AC:AF:NC	0:2:0.133333333333:A=2,C=13,	0:0:0:C=31,G=1,
+phiX174	1050	.	A	C	0	.	AC=3;AF=0.0714285714286	GT:AC:AF:NC	0:0:0:A=12,	0:3:0.1:A=27,C=3,
+phiX174	1051	.	T	G	0	.	AC=2;AF=0.0487804878049	GT:AC:AF:NC	0:2:0.166666666667:C=1,T=10,G=2,	0:0:0:T=29,G=1,
+phiX174	1054	.	T	G	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:2:0.153846153846:T=11,G=2,	0:0:0:T=31,G=1,
+phiX174	1076	.	G	C	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=2,G=17,	0:0:0:G=36,
+phiX174	1080	.	G	C	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=2,T=1,G=17,	0:0:0:C=1,G=39,
+phiX174	1092	.	T	G	0	.	AC=2;AF=0.0263157894737	GT:AC:AF:NC	0:2:0.0833333333333:C=1,T=22,G=2,	0:0:0:T=52,G=1,
+phiX174	1093	.	T	G	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.08:T=23,G=2,	0:0:0:T=52,
+phiX174	1102	.	T	C	0	.	AC=6;AF=0.0779220779221	GT:AC:AF:NC	0:2:0.0869565217391:C=2,T=21,	0:4:0.0740740740741:C=4,T=50,
+phiX174	1107	.	T	G	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:0:0:T=22,G=1,	0:2:0.04:T=48,G=2,
+phiX174	1110	.	T	C	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:2:0.125:C=2,T=14,	0:0:0:C=1,T=56,G=1,
+phiX174	1121	.	T	G	0	.	AC=3;AF=0.041095890411	GT:AC:AF:NC	0:0:0:T=19,	0:3:0.0555555555556:T=51,G=3,
+phiX174	1124	.	T	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0,2:0,0.117647058824:C=2,T=15,G=1,	0:0,0:0,0:T=48,G=1,
+phiX174	1125	.	T	G	0	.	AC=3;AF=0.0454545454545	GT:AC:AF:NC	0:3:0.176470588235:T=14,G=3,	0:0:0:T=49,G=1,
+phiX174	1131	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0952380952381:T=19,G=2,	0:0:0:T=57,
+phiX174	1141	.	T	G	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0:0:T=20,G=1,	0:2:0.0338983050847:T=57,G=2,
+phiX174	1145	.	G	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.0952380952381:C=2,G=19,	0:0:0:G=52,
+phiX174	1147	.	T	C	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0952380952381:C=2,T=19,	0:0:0:T=50,
+phiX174	1149	.	T	G,C	0	.	AC=2,2;AF=0.027027027027,0.027027027027	GT:AC:AF:NC	0:2,2:0.1,0.1:C=2,T=16,G=2,	0:0,0:0,0:T=54,
+phiX174	1156	.	T	G	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:0:0:C=1,T=15,	0:2:0.0416666666667:T=46,G=2,
+phiX174	1159	.	T	C,G	0	.	AC=2,2;AF=0.0298507462687,0.0298507462687	GT:AC:AF:NC	0:2,0:0.111111111111,0:C=2,T=16,	0:0,2:0,0.0408163265306:C=1,T=47,G=2,
+phiX174	1164	.	T	G	0	.	AC=4;AF=0.0666666666667	GT:AC:AF:NC	0:0:0:C=1,T=16,G=1,	0:4:0.0909090909091:T=40,G=4,
+phiX174	1168	.	T	G	0	.	AC=4;AF=0.0666666666667	GT:AC:AF:NC	0:2:0.0952380952381:C=1,T=19,G=2,	0:2:0.0512820512821:T=37,G=2,
+phiX174	1171	.	T	G	0	.	AC=2;AF=0.0327868852459	GT:AC:AF:NC	0:2:0.0869565217391:T=21,G=2,	0:0:0:T=38,G=1,
+phiX174	1174	.	C	A	0	.	AC=2;AF=0.0327868852459	GT:AC:AF:NC	0:2:0.0869565217391:A=2,C=21,	0:0:0:A=1,C=38,
+phiX174	1178	.	G	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:2:0.0740740740741:C=2,T=1,G=25,	0:0:0:G=38,
+phiX174	1187	.	T	G	0	.	AC=3;AF=0.04	GT:AC:AF:NC	0:3:0.0882352941176:C=1,T=31,G=3,	0:0:0:T=41,
+phiX174	1189	.	T	C	0	.	AC=3;AF=0.0379746835443	GT:AC:AF:NC	0:3:0.0857142857143:C=3,T=32,G=1,	0:0:0:T=44,
+phiX174	1200	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0882352941176:T=31,G=3,	0:0:0:T=48,G=1,
+phiX174	1202	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:0:0:T=32,	0:3:0.06:T=47,G=3,
+phiX174	1205	.	A	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.0588235294118:A=32,C=2,	0:0:0:A=47,C=1,G=1,
+phiX174	1207	.	T	G	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:A=1,T=34,	0:2:0.0425531914894:T=45,G=2,
+phiX174	1214	.	G	A	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:T=1,G=34,	0:2:0.0425531914894:A=2,G=45,
+phiX174	1216	.	C	A	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:C=34,	0:2:0.0425531914894:A=2,C=45,
+phiX174	1217	.	C	A	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.0588235294118:A=2,C=32,	0:0:0:C=46,
+phiX174	1219	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:0:0:C=1,T=33,G=1,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1223	.	C	T	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.0555555555556:C=34,T=2,	0:0:0:C=44,
+phiX174	1227	.	A	C	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0588235294118:A=32,C=2,	0:0:0:A=40,
+phiX174	1232	.	T	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:T=34,	0:2:0.0606060606061:C=1,T=31,G=2,
+phiX174	1236	.	G	T	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0606060606061:A=1,T=2,G=31,	0:0:0:G=35,
+phiX174	1237	.	T	G	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=1,T=33,G=2,	0:0:0:T=33,G=1,
+phiX174	1240	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:0,0:0,0:A=32,T=1,	0:0,2:0,0.0555555555556:A=34,C=2,T=1,
+phiX174	1245	.	G	T	0	.	AC=4;AF=0.0625	GT:AC:AF:NC	0:2:0.0666666666667:C=1,T=2,G=28,	0:2:0.0588235294118:T=2,G=32,
+phiX174	1247	.	A	T	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:2:0.0714285714286:A=26,C=1,T=2,G=1,	0:0:0:A=35,
+phiX174	1250	.	A	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:2:0.0769230769231:A=24,C=2,	0:0:0:A=39,
+phiX174	1251	.	A	G	0	.	AC=3;AF=0.044776119403	GT:AC:AF:NC	0:0:0:A=29,	0:3:0.0789473684211:A=35,C=1,G=3,
+phiX174	1253	.	T	G	0	.	AC=4;AF=0.0526315789474	GT:AC:AF:NC	0:2:0.0645161290323:T=29,G=2,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1257	.	T	G	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:0:0:T=26,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1259	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:A=28,T=1,	0:2:0.0444444444444:A=43,C=2,G=1,
+phiX174	1274	.	G	C	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.0512820512821:C=2,G=37,	0:0:0:G=44,
+phiX174	1277	.	A	C	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=40,G=1,
+phiX174	1279	.	T	G	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0:0:T=39,G=1,	0:2:0.05:C=1,T=38,G=2,
+phiX174	1284	.	T	G,C	0	.	AC=3,2;AF=0.038961038961,0.025974025974	GT:AC:AF:NC	0:3,2:0.075,0.05:C=2,T=35,G=3,	0:0,0:0,0:A=1,T=37,
+phiX174	1289	.	A	C	0	.	AC=3;AF=0.0454545454545	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=28,G=1,
+phiX174	1296	.	A	C	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:0:0:A=38,	0:2:0.0769230769231:A=24,C=2,
+phiX174	1298	.	A	C	0	.	AC=3;AF=0.0491803278689	GT:AC:AF:NC	0:3,0:0.0810810810811,0:A=34,C=3,G=1,	0:0,0:0,0:A=24,C=1,T=1,G=1,
+phiX174	1306	.	T	G	0	.	AC=5;AF=0.0943396226415	GT:AC:AF:NC	0:5:0.178571428571:T=23,G=5,	0:0:0:C=1,T=25,
+phiX174	1307	.	T	G	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.107142857143:T=25,G=3,	0:0:0:C=1,T=27,
+phiX174	1314	.	A	G	0	.	AC=5;AF=0.0877192982456	GT:AC:AF:NC	0:2,0:0.0740740740741,0:A=25,C=1,G=2,	0:3,0:0.1,0:A=27,C=1,G=3,
+phiX174	1317	.	A	C	0	.	AC=2;AF=0.0322580645161	GT:AC:AF:NC	0:2:0.0740740740741:A=25,C=2,	0:0:0:A=35,
+phiX174	1320	.	C	G	0	.	AC=2;AF=0.0322580645161	GT:AC:AF:NC	0:2:0.0769230769231:C=24,G=2,	0:0:0:C=36,
+phiX174	1322	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.107142857143:C=3,G=25,	0:0:0:G=40,
+phiX174	1323	.	C	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:C=28,	0:2:0.0512820512821:C=37,T=1,G=2,
+phiX174	1329	.	T	C	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:2:0.0666666666667:C=2,T=28,	0:0:0:T=42,G=1,
+phiX174	1335	.	C	A	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.0625:A=2,C=30,	0:0:0:C=45,
+phiX174	1338	.	T	A	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:0:0:T=32,	0:2:0.047619047619:A=2,T=40,
+phiX174	1345	.	T	C	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0,0:0,0:T=36,G=1,	0:0,2:0,0.046511627907:C=2,T=41,G=1,
+phiX174	1347	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0512820512821:A=37,C=2,G=1,	0:0:0:A=43,
+phiX174	1348	.	T	G	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.05:T=38,G=2,	0:0:0:T=43,
+phiX174	1352	.	A	T	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:A=34,G=1,	0:2:0.0512820512821:A=37,T=2,
+phiX174	1354	.	T	G,A	0	.	AC=2,2;AF=0.027027027027,0.027027027027	GT:AC:AF:NC	0:2,0:0.0526315789474,0:T=36,G=2,	0:0,2:0,0.0555555555556:A=2,T=34,G=1,
+phiX174	1359	.	T	G	0	.	AC=3;AF=0.0405405405405	GT:AC:AF:NC	0:3:0.0714285714286:T=39,G=3,	0:0:0:T=32,G=1,
+phiX174	1366	.	G	C	0	.	AC=3;AF=0.0428571428571	GT:AC:AF:NC	0:3:0.0714285714286:C=3,T=1,G=39,	0:0:0:G=28,
+phiX174	1370	.	T	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:0:0:C=1,T=45,G=1,	0:2:0.0833333333333:A=1,T=22,G=2,
+phiX174	1373	.	T	G	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0408163265306:T=47,G=2,	0:0:0:T=25,
+phiX174	1380	.	G	C	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:0,2:0,0.0384615384615:C=2,T=1,G=50,	0:0,0:0,0:T=1,G=32,
+phiX174	1381	.	T	G	0	.	AC=3;AF=0.0361445783133	GT:AC:AF:NC	0:3:0.0576923076923:A=1,T=49,G=3,	0:0:0:T=31,
+phiX174	1384	.	T	G	0	.	AC=4;AF=0.0512820512821	GT:AC:AF:NC	0:4:0.0816326530612:T=45,G=4,	0:0:0:T=29,G=1,
+phiX174	1385	.	T	G	0	.	AC=4;AF=0.0512820512821	GT:AC:AF:NC	0:4:0.0816326530612:T=45,G=4,	0:0:0:C=1,T=29,
+phiX174	1391	.	A	C	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:2:0.0408163265306:A=47,C=2,	0:0:0:A=30,C=1,
+phiX174	1393	.	C	A	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:2:0.0425531914894:A=2,C=45,	0:0:0:C=32,
+phiX174	1396	.	T	G	0	.	AC=3;AF=0.04	GT:AC:AF:NC	0:3:0.0697674418605:T=40,G=3,	0:0:0:T=32,
+phiX174	1397	.	A	C	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:0:0:A=42,T=1,	0:2:0.0625:A=30,C=2,
+phiX174	1400	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.047619047619:A=40,C=2,G=1,	0:0:0:A=31,
+phiX174	1401	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.047619047619:A=40,C=2,	0:0:0:A=31,
+phiX174	1434	.	C	T	0	.	AC=2;AF=0.0571428571429	GT:AC:AF:NC	0:2:0.142857142857:C=12,T=2,	0:0:0:C=21,
+phiX174	1438	.	G	C	0	.	AC=2;AF=0.0526315789474	GT:AC:AF:NC	0:0:0:G=15,	0:2:0.0869565217391:C=2,G=21,
+phiX174	1450	.	T	G	0	.	AC=3;AF=0.0789473684211	GT:AC:AF:NC	0:3:0.176470588235:A=1,C=1,T=14,G=3,	0:0:0:T=21,G=1,
+phiX174	1459	.	T	G	0	.	AC=3;AF=0.075	GT:AC:AF:NC	0:3:0.142857142857:C=1,T=18,G=3,	0:0:0:T=19,
+phiX174	1470	.	C	A	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:0:0:C=29,	0:2:0.0869565217391:A=2,C=21,
+phiX174	1484	.	C	G	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:2:0.0740740740741:A=1,C=25,G=2,	0:0:0:C=25,
+phiX174	1487	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0689655172414:T=27,G=2,	0:0:0:T=26,
+phiX174	1491	.	G	T	0	.	AC=2;AF=0.037037037037	GT:AC:AF:NC	0:0:0:T=1,G=26,	0:2:0.0714285714286:T=2,G=26,
+phiX174	1503	.	A	C	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:0:0:A=20,C=1,	0:2:0.0833333333333:A=22,C=2,
+phiX174	1536	.	A	C	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.111111111111:A=24,C=3,	0:0:0:A=28,C=1,
+phiX174	1541	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0740740740741:T=25,G=2,	0:0:0:T=28,
+phiX174	1553	.	A	G	0	.	AC=2;AF=0.0408163265306	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=1,G=2,	0:0:0:A=28,
+phiX174	1566	.	C	G	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0:0:C=30,T=1,	0:2:0.0571428571429:C=33,G=2,
+phiX174	1572	.	T	G	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:2:0.0625:T=30,G=2,	0:0:0:T=32,
+phiX174	1574	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0882352941176:C=3,G=31,	0:0:0:G=34,
+phiX174	1575	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=33,T=1,
+phiX174	1577	.	A	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:0:0:A=35,	0:3:0.0909090909091:A=30,C=3,
+phiX174	1581	.	T	G	0	.	AC=2;AF=0.0285714285714	GT:AC:AF:NC	0:2:0.0555555555556:C=1,T=34,G=2,	0:0:0:T=34,G=1,
+phiX174	1591	.	A	C	0	.	AC=3;AF=0.037037037037	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=43,C=1,
+phiX174	1597	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0606060606061:T=31,G=2,	0:0:0:C=1,T=45,
+phiX174	1603	.	T	G	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0625:T=30,G=2,	0:0:0:T=50,
+phiX174	1606	.	T	G	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0666666666667:A=1,C=1,T=28,G=2,	0:0:0:T=52,
+phiX174	1611	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0769230769231:A=24,C=2,	0:0:0:A=56,C=1,
+phiX174	1613	.	A	G	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:0:0:A=25,C=1,G=1,	0:2:0.0344827586207:A=56,G=2,
+phiX174	1618	.	C	A	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0666666666667:A=2,C=28,G=1,	0:0:0:A=1,C=57,
+phiX174	1619	.	C	A	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0666666666667:A=2,C=28,	0:0:0:C=57,
+phiX174	1621	.	A	C	0	.	AC=3;AF=0.0352941176471	GT:AC:AF:NC	0:0:0:A=31,T=1,G=1,	0:3:0.0555555555556:A=51,C=3,
+phiX174	1622	.	G	T	0	.	AC=2;AF=0.0224719101124	GT:AC:AF:NC	0:0:0:A=1,G=35,	0:2:0.037037037037:T=2,G=52,
+phiX174	1624	.	A	G	0	.	AC=2;AF=0.0235294117647	GT:AC:AF:NC	0:2:0.0555555555556:A=34,T=1,G=2,	0:0:0:A=49,
+phiX174	1627	.	T	G,C	0	.	AC=2,2;AF=0.0246913580247,0.0246913580247	GT:AC:AF:NC	0:0,0:0,0:T=37,G=1,	0:2,2:0.0454545454545,0.0454545454545:C=2,T=40,G=2,
+phiX174	1630	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0526315789474:C=1,T=36,G=2,	0:0:0:T=40,
+phiX174	1632	.	A	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=41,
+phiX174	1634	.	T	G	0	.	AC=4;AF=0.0493827160494	GT:AC:AF:NC	0:2:0.047619047619:C=1,T=40,G=2,	0:2:0.0512820512821:T=37,G=2,
+phiX174	1635	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0714285714286:T=39,G=3,	0:0:0:T=40,
+phiX174	1639	.	G	A	0	.	AC=3;AF=0.0405405405405	GT:AC:AF:NC	0:3:0.075:A=3,T=1,G=37,	0:0:0:G=34,
+phiX174	1645	.	T	G	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0425531914894:T=45,G=2,	0:0:0:T=27,G=1,
+phiX174	1647	.	A	T,G,C	0	.	AC=2,2,2;AF=0.0253164556962,0.0253164556962,0.0253164556962	GT:AC:AF:NC	0:0,2,2:0,0.0384615384615,0.0384615384615:A=48,C=2,G=2,	0:2,0,0:0.0740740740741,0,0:A=25,T=2,
+phiX174	1652	.	G	A	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.037037037037:A=2,G=52,	0:0:0:G=29,
+phiX174	1656	.	T	G	0	.	AC=3;AF=0.0348837209302	GT:AC:AF:NC	0:3:0.0555555555556:T=51,G=3,	0:0:0:T=32,G=1,
+phiX174	1658	.	A	C	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.04:A=48,C=2,	0:0:0:A=33,
+phiX174	1659	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0625:C=1,T=45,G=3,	0:0:0:T=34,
+phiX174	1663	.	T	G	0	.	AC=4;AF=0.0454545454545	GT:AC:AF:NC	0:0:0:A=1,T=53,	0:4:0.114285714286:T=31,G=4,
+phiX174	1665	.	C	A	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0384615384615:A=2,C=50,G=1,	0:0:0:C=42,
+phiX174	1670	.	G	C	0	.	AC=2;AF=0.0224719101124	GT:AC:AF:NC	0:0,2:0,0.0408163265306:A=1,C=2,T=1,G=47,	0:0,0:0,0:T=1,G=40,
+phiX174	1671	.	G	T	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0384615384615:C=1,T=2,G=50,	0:0:0:G=42,
+phiX174	1672	.	A	G	0	.	AC=3;AF=0.0315789473684	GT:AC:AF:NC	0:3:0.0545454545455:A=52,G=3,	0:0:0:A=40,T=1,
+phiX174	1673	.	G	T	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0377358490566:C=1,T=2,G=51,	0:0:0:G=41,
+phiX174	1674	.	G	T	0	.	AC=4;AF=0.04	GT:AC:AF:NC	0:4:0.0714285714286:T=4,G=52,	0:0:0:G=44,
+phiX174	1677	.	A	C	0	.	AC=3;AF=0.031914893617	GT:AC:AF:NC	0:3:0.0566037735849:A=50,C=3,N=1,	0:0:0:A=41,
+phiX174	1678	.	A	C	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:0:0:A=54,C=1,G=1,	0:2:0.05:A=38,C=2,
+phiX174	1682	.	T	C	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0408163265306:C=2,T=47,G=1,	0:0:0:C=1,T=38,
+phiX174	1685	.	T	G	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:2:0.046511627907:A=1,T=41,G=2,	0:0:0:T=41,G=1,
+phiX174	1686	.	A	C	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:0:0:A=39,C=1,T=1,G=1,	0:2:0.0512820512821:A=37,C=2,
+phiX174	1687	.	T	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.047619047619:C=2,T=40,	0:0:0:A=1,T=40,
+phiX174	1688	.	G	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.05:C=2,T=1,G=38,	0:0:0:G=41,
+phiX174	1689	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0487804878049:A=39,C=2,T=1,G=1,	0:0:0:A=41,C=1,
+phiX174	1691	.	G	C	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.05:C=2,T=1,G=38,	0:0:0:G=37,
+phiX174	1693	.	T	G	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:0:0:T=42,G=1,	0:2:0.047619047619:T=40,G=2,
+phiX174	1695	.	A	C	0	.	AC=2;AF=0.0232558139535	GT:AC:AF:NC	0:2:0.046511627907:A=41,C=2,	0:0:0:A=43,C=1,
+phiX174	1702	.	T	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.0512820512821:A=1,C=2,T=37,	0:0:0:C=1,T=42,
+phiX174	1707	.	T	C	0	.	AC=2;AF=0.0232558139535	GT:AC:AF:NC	0:2:0.05:C=2,T=38,	0:0:0:T=46,G=1,
+phiX174	1708	.	A	C	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:2:0.0526315789474:A=36,C=2,	0:0:0:A=46,C=1,G=1,
+phiX174	1710	.	T	C	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:0:0:C=1,T=34,	0:2:0.0434782608696:C=2,T=44,
+phiX174	1723	.	T	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0588235294118:C=2,T=32,	0:0:0:T=48,
+phiX174	1724	.	A	C	0	.	AC=4;AF=0.0481927710843	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:2:0.0416666666667:A=46,C=2,
+phiX174	1725	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0606060606061:A=31,C=2,	0:0:0:A=49,
+phiX174	1726	.	T	G	0	.	AC=3;AF=0.037037037037	GT:AC:AF:NC	0:3:0.0882352941176:T=31,G=3,	0:0:0:C=1,T=47,
+phiX174	1730	.	T	G	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:C=1,T=28,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1739	.	G	T	0	.	AC=3;AF=0.0434782608696	GT:AC:AF:NC	0:3:0.09375:T=3,G=29,	0:0:0:G=37,
+phiX174	1746	.	A	C	0	.	AC=2;AF=0.0338983050847	GT:AC:AF:NC	0:2:0.0689655172414:A=27,C=2,	0:0:0:A=30,C=1,G=1,
+phiX174	1749	.	T	G,C	0	.	AC=3,2;AF=0.044776119403,0.0298507462687	GT:AC:AF:NC	0:3,2:0.0857142857143,0.0571428571429:C=2,T=30,G=3,	0:0,0:0,0:T=32,G=1,
+phiX174	1755	.	G	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.05:C=2,G=38,	0:0:0:C=1,G=33,
+phiX174	1756	.	A	G,C	0	.	AC=2,2;AF=0.0281690140845,0.0281690140845	GT:AC:AF:NC	0:0,2:0,0.0526315789474:A=36,C=2,T=1,G=1,	0:2,0:0.0606060606061,0:A=31,C=1,G=2,
+phiX174	1761	.	A	G	0	.	AC=2;AF=0.0285714285714	GT:AC:AF:NC	0:2:0.0512820512821:A=37,C=1,G=2,	0:0:0:A=31,
+phiX174	1764	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=28,
+phiX174	1766	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0487804878049:A=39,C=2,	0:0:0:A=27,
+phiX174	1769	.	T	G	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:2:0.0526315789474:A=1,C=1,T=36,G=2,	0:0:0:T=26,G=1,
+phiX174	1771	.	G	C	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:2:0.0526315789474:C=2,G=36,	0:0:0:G=29,
+phiX174	1772	.	T	G	0	.	AC=4;AF=0.0597014925373	GT:AC:AF:NC	0:4:0.108108108108:C=1,T=33,G=4,	0:0:0:T=30,
+phiX174	1774	.	A	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0810810810811:A=34,G=3,	0:0:0:A=31,
+phiX174	1775	.	G	A	0	.	AC=2;AF=0.030303030303	GT:AC:AF:NC	0:2:0.0571428571429:A=2,G=33,	0:0:0:G=31,
+phiX174	1776	.	G	T	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0540540540541:T=2,G=35,	0:0:0:G=32,
+phiX174	1778	.	C	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0526315789474:C=36,G=2,	0:0:0:C=31,
+phiX174	1779	.	A	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.075:A=37,C=3,	0:0:0:A=28,
+phiX174	1781	.	T	G	0	.	AC=5;AF=0.0714285714286	GT:AC:AF:NC	0:2:0.0487804878049:C=1,T=39,G=2,	0:3:0.103448275862:T=26,G=3,
+phiX174	1782	.	T	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.075:T=37,G=3,	0:0:0:A=1,T=28,
+phiX174	1783	.	T	C	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:2:0.0512820512821:C=2,T=37,	0:0:0:T=28,G=1,
+phiX174	1788	.	G	T	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:0:0:A=1,T=1,G=29,	0:2:0.0689655172414:T=2,G=27,
+phiX174	1792	.	T	G	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:2:0.0606060606061:T=31,G=2,	0:0:0:T=25,G=1,
+phiX174	1802	.	A	C	0	.	AC=2;AF=0.030303030303	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=31,C=1,
+phiX174	1804	.	C	T	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0588235294118:A=1,C=32,T=2,	0:0:0:C=34,
+phiX174	1805	.	T	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0857142857143:T=32,G=3,	0:0:0:T=33,G=1,
+phiX174	1806	.	A	C	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=34,G=1,
+phiX174	1824	.	G	C	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0714285714286:C=2,T=1,G=26,	0:0:0:G=43,
+phiX174	1825	.	T	G	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0689655172414:T=27,G=2,	0:0:0:T=42,G=1,
+phiX174	1833	.	T	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:0:0:A=1,T=24,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1843	.	T	G	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:2:0.08:A=1,T=23,G=2,	0:0:0:T=38,
+phiX174	1847	.	A	C	0	.	AC=2;AF=0.0333333333333	GT:AC:AF:NC	0:2:0.0833333333333:A=22,C=2,T=1,G=1,	0:0:0:A=36,
+phiX174	1854	.	T	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:T=26,	0:2:0.0487804878049:T=39,G=2,
+phiX174	1860	.	T	G	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:0:0:A=1,T=20,	0:2:0.0606060606061:T=31,G=2,
+phiX174	1863	.	C	G	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=17,G=2,	0:0:0:C=33,
+phiX174	1866	.	T	G	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:0:0:T=19,G=1,	0:2:0.0645161290323:T=29,G=2,
+phiX174	1875	.	T	A	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:2:0.0952380952381:A=2,T=19,	0:0:0:T=32,G=1,
+phiX174	1876	.	T	G	0	.	AC=3;AF=0.0588235294118	GT:AC:AF:NC	0:3:0.157894736842:C=1,T=16,G=3,	0:0:0:T=32,G=1,
+phiX174	1882	.	T	G	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:2:0.105263157895:T=17,G=2,	0:0:0:T=34,
+phiX174	1889	.	A	C	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:2:0.0869565217391:A=21,C=2,	0:0:0:A=27,
+phiX174	1913	.	G	C	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:0:0:G=27,	0:2:0.0869565217391:C=2,G=21,
+phiX174	1915	.	T	G	0	.	AC=2;AF=0.0425531914894	GT:AC:AF:NC	0:0:0:A=1,T=24,	0:2:0.0869565217391:T=21,G=2,
+phiX174	1917	.	A	T	0	.	AC=2;AF=0.0444444444444	GT:AC:AF:NC	0:2,0:0.0833333333333,0:A=22,T=2,G=1,	0:0,0:0,0:A=21,G=1,
+phiX174	1930	.	T	A	0	.	AC=2;AF=0.0526315789474	GT:AC:AF:NC	0:2:0.0909090909091:A=2,T=20,	0:0:0:T=16,
+phiX174	1934	.	A	C	0	.	AC=2;AF=0.0512820512821	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=2,T=1,	0:0:0:A=18,C=1,
+phiX174	1940	.	A	C	0	.	AC=2;AF=0.046511627907	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=2,	0:0:0:A=22,C=1,
+phiX174	1942	.	T	G	0	.	AC=2;AF=0.047619047619	GT:AC:AF:NC	0:0:0:T=19,	0:2:0.0869565217391:T=21,G=2,
+phiX174	1945	.	T	G	0	.	AC=3;AF=0.0769230769231	GT:AC:AF:NC	0:0:0:T=15,G=1,	0:3:0.125:T=21,G=3,
+phiX174	1954	.	T	C	0	.	AC=2;AF=0.0625	GT:AC:AF:NC	0:0,0:0,0:T=12,G=1,	0:0,2:0,0.1:C=2,T=18,G=1,
+phiX174	1959	.	T	G	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:2:0.153846153846:T=11,G=2,	0:0:0:T=23,
+phiX174	1994	.	A	C	0	.	AC=2;AF=0.0512820512821	GT:AC:AF:NC	0:2:0.153846153846:A=11,C=2,	0:0:0:A=26,
+phiX174	1998	.	A	G	0	.	AC=2;AF=0.05	GT:AC:AF:NC	0:2:0.142857142857:A=12,G=2,	0:0:0:A=26,
diff --git a/tool_collections/vcflib/vcfgeno2haplo/test-data/vcflib-test-genome-phix.fa b/tool_collections/vcflib/vcfgeno2haplo/test-data/vcflib-test-genome-phix.fa
new file mode 100644
index 00000000000..48a6b46678a
--- /dev/null
+++ b/tool_collections/vcflib/vcfgeno2haplo/test-data/vcflib-test-genome-phix.fa
@@ -0,0 +1,2 @@
+>phiX174
+GAGTTTTATCGCTTCCATGACGCAGAAGTTAACACTTTCGGATATTTCTGATGAGTCGAAAAATTATCTTGATAAAGCAGGAATTACTACTGCTTGTTTACGAATTAAATCGAAGTGGACTGCTGGCGGAAAATGAGAAAATTCGACCTATCCTTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGTTTAGATATGAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTGGATTACTATCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAGAAATCATGAGTCAAGTTACTGAACAATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTAACCGAAGATGATTTCGATTTTCTGACGAGTAACAAAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCTTGCGTTTATGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTTTATTGCTGCCGTCATTGCTTATTATGTTCATCCCGTCAACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTACGGAAAACATTATTAATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAACACTGACGTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAAATTACGTGCaGAAGGAGTGATGTAATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATTTCAACTACTCCGGTTATCGCTGGCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTTTTTATGTCCCTCATCGTCACGTTTATGGTGAACAGTGGATTAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCCGACTGTTAACACTACTGGTTATATTGACCATGCCGCTTTTCTTGGCACGATTAACCCTGATACCAATAAAATCCCTAAGCATTTGTTTCAGGGTTATTTGAATATCTATAACAACTATTTTAAAGCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATCAAGATGATGCTCGTTATGGTTTCCGTTGCTGCCATCTCAAAAACATTTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGACTTCTACCACATCTATTGACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGCATACTGACCAAGAACGTGATTACTTCATGCAGCGTTACCgTGATGTTATTTCTTCATTTGGAGGTAAAACCTCTTATGACGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCTATGATGTTGATGGAACTGACCAAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAACATTCTGTGCCGCGTTTCTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGCGACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGCTTTGACTTATACCGATATTGCTGGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTGCTGAGGGTCAGTGGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGATTATGACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGAATAGTCAGGTTAAATTTAATGTGACCGTTTATCGCAATCTGCCGACCACTCGCGATTCAATCATGACTTCGTGATAAAAGATTGAGTGTGAGGTTATAACGCCGAAGCGGTAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACCAAGCGAAGCGCGGTAGGTTTTCTGCTTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTCGCCATAATTCAAACTTTTTTTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTTTACAGACACCTAAAGCTACATCGTCAACGTTATATTTTGATAGTTTGACGGTTAATGCTGGTAATGGTGGTTTTCTTCATTGCATTCAGATGGATACATCTGTCAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGCCGACCCTAAATTTTTTGCCTGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTCCCGACTGCCTATGATGTTTATCCTTTGAATGGTCGCCATGATGGTGGTTATTATACCGTCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTACGCCGGGCAATAAtGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGGTGATTTATGTTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAAAGCCGCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACCGATAACAATACTGTAGGCATGGGTGATGCTGGTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCAACTGGACAATCAGAAAGAGATTGCCGAGATGCAAAATGAGACTCAAAAAGAGATTGCTGGCATTCAGTCGGCGACTTCACGCCAGAATACGAAAGACCAGGTATATGCACAAAATGAGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGCGTTGCGTCTATTATGGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAAGCTCAAACGGCTGGTCAGTATTTTACCAATGACCAAATCAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCTCTTCTCATATTGGCGCTACTGCAAAGGATATTTCTAATGTCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTTTTCATGGTATTGATAAAGCTGTTGCCGATACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTCTAATTTGTCTAGGAAATAACCGTCAGGATTGACACCCTCCCAATTGTATGTTTTCATGCCTCCAAATCTTGGAGGCTTTTTTATGGTTCGTTCTTATTACCCTTCTGAATGTCACGCTGATTATTTTGACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAATGCTTGGCTTCCATAAGCAGATGGATAACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAGGGCGTTGAGTTCGATAATGGTGATATGTATGTTGACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTACTGAGAAGTTAATGGATGAATTGGCACAATGCTACAATGTGCTCCCCCAACTTGATATTAATAACACTATAGACCACCGCCCCGAAGGGGACGAAAAATGGTTTTTAGAGAACGAGAAGACGGTTACGCAGTTTTGCCGCAAGCTGGCTGCTGAACGCCCTCTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTaCTATTCAGCGTTTGATGAATGCAATGCGACAGGCTCATGCTGATGGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCTGACGACCGATTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGTGACTATTTTCGTGATATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATGATTCACACGCCGACTGCTATCAGTATTTTTGTGTGCCTGAGTATGGTACAGCTAATGGCCGTCTTCATTTCCATGCGGTGCAtTTTATGCGGACACTTCCTACAGGTAGCGTTGACCCTAATTTTGGTCGTCGGGTACGCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGTGGCCTTATGGTTACAGTATGCCCATCGCAGTTCGCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTAAATACGTTAACAAAAAGTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCACTAAAAACCAAGCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAGAATGAGCCGCAACTTCGGGATGAAAATGCTCACAATGACAAATCTGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGATATTGAAGCAGAACGCAAAAAGAGAGATGAGATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTTTGGCGGCGCAACCTGTGACGACAAATCTGCTCAAATTTATGCGCGCTTCGATAAAAATGATTGGCGTATCCAACCTGCA
diff --git a/tool_collections/vcflib/vcfgeno2haplo/tool-data/fasta_indexes.loc.sample b/tool_collections/vcflib/vcfgeno2haplo/tool-data/fasta_indexes.loc.sample
new file mode 120000
index 00000000000..6d7aec6c449
--- /dev/null
+++ b/tool_collections/vcflib/vcfgeno2haplo/tool-data/fasta_indexes.loc.sample
@@ -0,0 +1 @@
+../../tool-data/fasta_indexes.loc.sample
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfgeno2haplo/tool-data/tool_data_table_conf.xml.sample b/tool_collections/vcflib/vcfgeno2haplo/tool-data/tool_data_table_conf.xml.sample
new file mode 120000
index 00000000000..7bfcf015ca6
--- /dev/null
+++ b/tool_collections/vcflib/vcfgeno2haplo/tool-data/tool_data_table_conf.xml.sample
@@ -0,0 +1 @@
+../../tool-data/tool_data_table_conf.xml.sample
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfgeno2haplo/vcfgeno2haplo.xml b/tool_collections/vcflib/vcfgeno2haplo/vcfgeno2haplo.xml
new file mode 100644
index 00000000000..a2c2d1d8c7d
--- /dev/null
+++ b/tool_collections/vcflib/vcfgeno2haplo/vcfgeno2haplo.xml
@@ -0,0 +1,75 @@
+<tool id="vcfgeno2haplo" name="VCFgenotype-to-haplotype:" version="@WRAPPER_VERSION@.0">
+  <description>Convert genotype-based phased alleles into haplotype alleles</description>
+  <macros>
+    <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"/>
+  <expand macro="stdio" />
+  <command>
+    #set $reference_fasta_filename = "localref.fa"
+    #if str( $reference_source.reference_source_selector ) == "history":
+       ln -s "${reference_source.ref_file}" "${reference_fasta_filename}" &amp;&amp;
+    #else:
+       #set $reference_fasta_filename = str( $reference_source.ref_file.fields.path )
+    #end if    
+  vcfgeno2haplo ${output_option} -w ${window_size} -r "${reference_fasta_filename}" "${input_vcf}" > "${out_file1}"</command>
+  <inputs>
+    <conditional name="reference_source">
+       <param name="reference_source_selector" type="select" label="Choose the source for the reference genome">
+         <option value="cached">Locally cached</option>
+         <option value="history">History</option>
+       </param>
+       <when value="cached">
+         <param name="ref_file" type="select" label="Select reference genome">
+           <options from_data_table="fasta_indexes">
+             <!--<filter type="data_meta" key="dbkey" ref="input_bam" column="value"/>-->
+           </options>
+	   <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/>
+         </param>
+	 <param name="input_vcf" type="data" format="vcf" label="Select VCF dataset">
+	   <!-- Validators are commented to allow users apply too to any build. May need to be revised in the future
+		<validator type="unspecified_build" />
+		<validator type="dataset_metadata_in_data_table" table_name="fasta_indexes" metadata_name="dbkey" metadata_column="1" message="Sequences are not currently available for the specified build." />
+	   -->
+	 </param>
+       </when>
+       <when value="history"> <!-- FIX ME!!!! -->
+         <param name="ref_file" type="data" format="fasta" label="Using reference file" />
+	 <param name="input_vcf" type="data" format="vcf" label="Select VCF dataset" />
+       </when>
+     </conditional>
+     <param name="window_size" type="text" value="30" label="compare records up to this many bp away" help="--window-size option (default = 30)" />
+     <param name="output_option" type="boolean" truevalue="-o" falsevalue="" label="What to report?" help="--only-variants option" />
+<!--       <option value=" ">Output entire haplotype</option>
+       <option value="-o">Don't output the entire haplotype, just concatenate REF/ALT strings (delimited by &quot;:&quot;)</option>
+     </param> -->
+  </inputs>
+  <outputs>
+    <data format="vcf" name="out_file1" />
+  </outputs>
+  <tests>
+    <test>
+      <param name="reference_source_selector" value="history" />
+      <param name="output_option" value="true" />
+      <param name="window_size" value="5000" />
+      <param name="input_vcf" value="vcflib-phix.vcf"/>
+      <param name="ref_file" value="vcflib-test-genome-phix.fa" />
+      <output name="out_file1" file="vcfgeno2haplo-test1.vcf"/>
+    </test>
+    </tests>
+  <help>
+
+Convert genotype-based phased alleles within a window size specified by -w option into haplotype alleles. Will break haplotype construction when encountering non-phased genotypes on input.
+
+The options are::
+
+    -w, --window-size N     Merge variants at most this many bp apart (default 30)
+    -o, --only-variants     Don't output the entire haplotype, just concatenate
+                            REF/ALT strings (delimited by ":")
+
+----
+
+Vcfgeno2haplo @IS_PART_OF_VCFLIB@
+</help>
+  <expand macro="citations" />
+</tool>
diff --git a/tool_collections/vcflib/vcfgenotypes/.shed.yml b/tool_collections/vcflib/vcfgenotypes/.shed.yml
new file mode 100644
index 00000000000..58c1a1b3d2e
--- /dev/null
+++ b/tool_collections/vcflib/vcfgenotypes/.shed.yml
@@ -0,0 +1,11 @@
+categories:
+- Variant Analysis
+description: Convert numerical representation of genotypes to allelic.
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  Converts numerical representation of genotypes (standard in GT
+  field) to the alleles provided in the call''s ALT/REF fields.
+name: vcfgenotypes
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfgenotypes
+type: unrestricted
diff --git a/tool_collections/vcflib/vcfgenotypes/macros.xml b/tool_collections/vcflib/vcfgenotypes/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcfgenotypes/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfgenotypes/test-data/vcfgenotypes-test1.tab b/tool_collections/vcflib/vcfgenotypes/test-data/vcfgenotypes-test1.tab
new file mode 100644
index 00000000000..5d5b50d6064
--- /dev/null
+++ b/tool_collections/vcflib/vcfgenotypes/test-data/vcfgenotypes-test1.tab
@@ -0,0 +1,9 @@
+19	111	A	C	A,C	NA00001:A/A	NA00002:A/A	NA00003:A/C	
+19	112	A	G	A,G	NA00001:A/A	NA00002:A/A	NA00003:A/G	
+20	14370	G	A	G,A	NA00001:G/G	NA00002:A/G	NA00003:A/A	
+20	17330	T	A	T,A	NA00001:T/T	NA00002:T/A	NA00003:T/T	
+20	1110696	A	G,T	A,G,T	NA00001:G/T	NA00002:T/G	NA00003:T/T	
+20	1230237	T	.	T,.	NA00001:T/T	NA00002:T/T	NA00003:T/T	
+20	1234567	G	GA,GAC	G,GA,GAC	NA00001:G/GA	NA00002:G/GAC	NA00003:GA/GA	
+20	1235237	T	.	T,.	NA00001:T/T	NA00002:T/T	NA00003:T/T	
+X	10	AC	A,ATG	AC,A,ATG	NA00001:AC	NA00002:AC/A	NA00003:AC/ATG	
diff --git a/tool_collections/vcflib/vcfgenotypes/test-data/vcflib.vcf b/tool_collections/vcflib/vcfgenotypes/test-data/vcflib.vcf
new file mode 100644
index 00000000000..e8dd794d9bd
--- /dev/null
+++ b/tool_collections/vcflib/vcfgenotypes/test-data/vcflib.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	NS=3;DP=14;AF=0.5;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	NS=3;DP=11;AF=0.017	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	NS=2;DP=10;AF=0.333,0.667;AA=T;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	NS=3;DP=13;AA=T	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	NS=3;DP=9;AA=G;AN=6;AC=3,1	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	.	.	.	GT	0/0	0|0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcfgenotypes/vcfgenotypes.xml b/tool_collections/vcflib/vcfgenotypes/vcfgenotypes.xml
new file mode 100644
index 00000000000..ccf7d476db6
--- /dev/null
+++ b/tool_collections/vcflib/vcfgenotypes/vcfgenotypes.xml
@@ -0,0 +1,30 @@
+<tool id="vcfgenotypes" name="VCFgenotypes:" version="@WRAPPER_VERSION@.0">
+  <description>Convert numerical representation of genotypes to allelic</description>
+  <macros>
+    <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"/>
+  <expand macro="stdio" />
+  <command>vcfgenotypes "${vcf_input}" > "${out_file1}"</command>
+  <inputs>
+    <param format="vcf" name="vcf_input" type="data" label="Select VCF dataset"/>
+  </inputs>
+  <outputs>
+    <data format="tabular" name="out_file1" />
+  </outputs>
+  <tests>
+    <test>
+      <param name="vcf_input" value="vcflib.vcf"/>
+      <output name="out_file1" file="vcfgenotypes-test1.tab"/>
+    </test>
+    </tests>
+  <help>
+
+Converts numerical representation of genotypes (standard in GT field) to the alleles provided in the call's ALT/REF fields.
+
+----
+
+Vcfgenotypes @IS_PART_OF_VCFLIB@
+</help>
+  <expand macro="citations" />
+</tool>
diff --git a/tool_collections/vcflib/vcfhethom/.shed.yml b/tool_collections/vcflib/vcfhethom/.shed.yml
new file mode 100644
index 00000000000..6980ba57fa6
--- /dev/null
+++ b/tool_collections/vcflib/vcfhethom/.shed.yml
@@ -0,0 +1,14 @@
+categories:
+- Variant Analysis
+description: 'Count the number of heterozygotes and alleles, compute het/hom ratio.'
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  This tool perfoms three basic calculations:
+
+  (1) Computes the number of heterozygotes
+  (2) Computes the ratio between heterozygotes and homozygotes
+  (3) Computes the total number of alleles in the input dataset
+name: vcfhethom
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfhethom
+type: unrestricted
diff --git a/tool_collections/vcflib/vcfhethom/macros.xml b/tool_collections/vcflib/vcfhethom/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcfhethom/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfhethom/test-data/vcfhethom-test1.tab b/tool_collections/vcflib/vcfhethom/test-data/vcfhethom-test1.tab
new file mode 100644
index 00000000000..bc3c3b9c672
--- /dev/null
+++ b/tool_collections/vcflib/vcfhethom/test-data/vcfhethom-test1.tab
@@ -0,0 +1,2 @@
+NA00001	NA00002	NA00003
+1	4	3
diff --git a/tool_collections/vcflib/vcfhethom/test-data/vcfhethom-test2.tab b/tool_collections/vcflib/vcfhethom/test-data/vcfhethom-test2.tab
new file mode 100644
index 00000000000..307bec64b2b
--- /dev/null
+++ b/tool_collections/vcflib/vcfhethom/test-data/vcfhethom-test2.tab
@@ -0,0 +1,2 @@
+NA00001	NA00002	NA00003
+inf	inf	0.75
diff --git a/tool_collections/vcflib/vcfhethom/test-data/vcfhethom-test3.tab b/tool_collections/vcflib/vcfhethom/test-data/vcfhethom-test3.tab
new file mode 100644
index 00000000000..aabe6ec3909
--- /dev/null
+++ b/tool_collections/vcflib/vcfhethom/test-data/vcfhethom-test3.tab
@@ -0,0 +1 @@
+21
diff --git a/tool_collections/vcflib/vcfhethom/test-data/vcflib.vcf b/tool_collections/vcflib/vcfhethom/test-data/vcflib.vcf
new file mode 100644
index 00000000000..e8dd794d9bd
--- /dev/null
+++ b/tool_collections/vcflib/vcfhethom/test-data/vcflib.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	NS=3;DP=14;AF=0.5;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	NS=3;DP=11;AF=0.017	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	NS=2;DP=10;AF=0.333,0.667;AA=T;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	NS=3;DP=13;AA=T	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	NS=3;DP=9;AA=G;AN=6;AC=3,1	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	.	.	.	GT	0/0	0|0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcfhethom/vcfhethom.xml b/tool_collections/vcflib/vcfhethom/vcfhethom.xml
new file mode 100644
index 00000000000..60cce1b877d
--- /dev/null
+++ b/tool_collections/vcflib/vcfhethom/vcfhethom.xml
@@ -0,0 +1,61 @@
+<tool id="vcfhethom" name="VCFhetHomAlleles:" version="@WRAPPER_VERSION@.0">
+  <description>Count the number of heterozygotes and alleles, compute het/hom ratio</description>
+  <macros>
+    <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"/>
+  <expand macro="stdio" />
+  <command>
+
+   #if str($analysis_type) == "count":
+       vcfhetcount "${vcf_input}" > "${out_file1}"
+   #elif str($analysis_type) == "ratio": 
+       vcfhethomratio "${vcf_input}" > "${out_file1}"
+   #elif str($analysis_type) == "allele_count":
+       vcfcountalleles "${vcf_input}" > "${out_file1}" 
+   #end if
+
+  </command>
+  <inputs>
+    <param name="analysis_type" type="select" display="radio" label="Select type of calculation:">
+      <option value="count">Compute the number of heterozygotes</option>
+      <option value="ratio">Compute the ratio between heterozygotes and homozygotes</option>
+      <option value="allele_count">Compute the total number of alleles</option>
+    </param>
+    <param format="vcf" name="vcf_input" type="data" label="In this VCF dataset"/>
+  </inputs>
+  <outputs>
+    <data format="tabular" name="out_file1" />
+  </outputs>
+  <tests>
+    <test>
+      <param name="analysis_type" value="count"/>
+      <param name="vcf_input" value="vcflib.vcf"/>
+      <output name="out_file1" file="vcfhethom-test1.tab"/>
+    </test>
+    <test>
+      <param name="analysis_type" value="ratio"/>
+      <param name="vcf_input" value="vcflib.vcf"/>
+      <output name="out_file1" file="vcfhethom-test2.tab"/>
+    </test>
+    <test>
+      <param name="analysis_type" value="allele_count"/>
+      <param name="vcf_input" value="vcflib.vcf"/>
+      <output name="out_file1" file="vcfhethom-test3.tab"/>
+    </test>
+  </tests>
+  <help>
+
+This tool performs three basic calculations: 
+
+(1) Computes the number of heterozygotes
+(2) Computes the ratio between heterozygotes and homozygotes
+(3) Computes the total number of alleles in the input dataset
+
+----
+
+This tools is based on vcfhetcount, vcfhethomratio,and vcfcountalleles utilities from the VCFlib toolkit developed by Erik Garrison (https://github.com/ekg/vcflib).                                                                                                                                 
+
+</help>
+  <expand macro="citations" />
+</tool>
diff --git a/tool_collections/vcflib/vcfleftalign/.shed.yml b/tool_collections/vcflib/vcfleftalign/.shed.yml
new file mode 100644
index 00000000000..497c1134cec
--- /dev/null
+++ b/tool_collections/vcflib/vcfleftalign/.shed.yml
@@ -0,0 +1,12 @@
+categories:
+- Variant Analysis
+description: Left-align indels and complex variants in VCF dataset
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  Left-aligns variants in VCF dataset.  Window size is determined
+  dynamically according to the entropy of the regions flanking the indel.  These must
+  have entropy > 1 bit/bp, or be shorter than ~5kb.
+name: vcfleftalign
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfleftalign
+type: unrestricted
diff --git a/tool_collections/vcflib/vcfleftalign/macros.xml b/tool_collections/vcflib/vcfleftalign/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcfleftalign/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfleftalign/test-data/vcfleftalign-test1.vcf b/tool_collections/vcflib/vcfleftalign/test-data/vcfleftalign-test1.vcf
new file mode 100644
index 00000000000..362e6be2088
--- /dev/null
+++ b/tool_collections/vcflib/vcfleftalign/test-data/vcfleftalign-test1.vcf
@@ -0,0 +1,219 @@
+##fileformat=VCFv4.1
+##fileDate=20140610
+##source=Dan
+##reference=file:///galaxy/data/phiX/sam_index/phiX.fa
+##filter="AF > .001"
+##INFO=<ID=AC,Number=A,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=AF,Number=A,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=AF,Number=.,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=NC,Number=.,Type=String,Description="Nucleotide and indel counts">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	groupA	groupB
+phiX174	1015	.	A	C	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:0,2:0,0.2:A=8,C=2,G=1,	0:0,0:0,0:A=26,G=1,
+phiX174	1016	.	A	T	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:0:0:A=10,	0:2:0.0769230769231:A=24,T=2,
+phiX174	1038	.	A	C	0	.	AC=2;AF=0.046511627907	GT:AC:AF:NC	0:2:0.153846153846:A=11,C=2,	0:0:0:A=30,
+phiX174	1045	.	T	G	0	.	AC=2;AF=0.0425531914894	GT:AC:AF:NC	0:0:0:T=15,G=1,	0:2:0.0625:T=30,G=2,
+phiX174	1048	.	C	A	0	.	AC=2;AF=0.0434782608696	GT:AC:AF:NC	0:2:0.133333333333:A=2,C=13,	0:0:0:C=31,G=1,
+phiX174	1050	.	A	C	0	.	AC=3;AF=0.0714285714286	GT:AC:AF:NC	0:0:0:A=12,	0:3:0.1:A=27,C=3,
+phiX174	1051	.	T	G	0	.	AC=2;AF=0.0487804878049	GT:AC:AF:NC	0:2:0.166666666667:C=1,T=10,G=2,	0:0:0:T=29,G=1,
+phiX174	1054	.	T	G	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:2:0.153846153846:T=11,G=2,	0:0:0:T=31,G=1,
+phiX174	1076	.	G	C	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=2,G=17,	0:0:0:G=36,
+phiX174	1080	.	G	C	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=2,T=1,G=17,	0:0:0:C=1,G=39,
+phiX174	1092	.	T	G	0	.	AC=2;AF=0.0263157894737	GT:AC:AF:NC	0:2:0.0833333333333:C=1,T=22,G=2,	0:0:0:T=52,G=1,
+phiX174	1093	.	T	G	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.08:T=23,G=2,	0:0:0:T=52,
+phiX174	1102	.	T	C	0	.	AC=6;AF=0.0779220779221	GT:AC:AF:NC	0:2:0.0869565217391:C=2,T=21,	0:4:0.0740740740741:C=4,T=50,
+phiX174	1107	.	T	G	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:0:0:T=22,G=1,	0:2:0.04:T=48,G=2,
+phiX174	1110	.	T	C	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:2:0.125:C=2,T=14,	0:0:0:C=1,T=56,G=1,
+phiX174	1121	.	T	G	0	.	AC=3;AF=0.041095890411	GT:AC:AF:NC	0:0:0:T=19,	0:3:0.0555555555556:T=51,G=3,
+phiX174	1124	.	T	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0,2:0,0.117647058824:C=2,T=15,G=1,	0:0,0:0,0:T=48,G=1,
+phiX174	1125	.	T	G	0	.	AC=3;AF=0.0454545454545	GT:AC:AF:NC	0:3:0.176470588235:T=14,G=3,	0:0:0:T=49,G=1,
+phiX174	1131	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0952380952381:T=19,G=2,	0:0:0:T=57,
+phiX174	1141	.	T	G	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0:0:T=20,G=1,	0:2:0.0338983050847:T=57,G=2,
+phiX174	1145	.	G	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.0952380952381:C=2,G=19,	0:0:0:G=52,
+phiX174	1147	.	T	C	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0952380952381:C=2,T=19,	0:0:0:T=50,
+phiX174	1149	.	T	G,C	0	.	AC=2,2;AF=0.027027027027,0.027027027027	GT:AC:AF:NC	0:2,2:0.1,0.1:C=2,T=16,G=2,	0:0,0:0,0:T=54,
+phiX174	1156	.	T	G	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:0:0:C=1,T=15,	0:2:0.0416666666667:T=46,G=2,
+phiX174	1159	.	T	C,G	0	.	AC=2,2;AF=0.0298507462687,0.0298507462687	GT:AC:AF:NC	0:2,0:0.111111111111,0:C=2,T=16,	0:0,2:0,0.0408163265306:C=1,T=47,G=2,
+phiX174	1164	.	T	G	0	.	AC=4;AF=0.0666666666667	GT:AC:AF:NC	0:0:0:C=1,T=16,G=1,	0:4:0.0909090909091:T=40,G=4,
+phiX174	1168	.	T	G	0	.	AC=4;AF=0.0666666666667	GT:AC:AF:NC	0:2:0.0952380952381:C=1,T=19,G=2,	0:2:0.0512820512821:T=37,G=2,
+phiX174	1171	.	T	G	0	.	AC=2;AF=0.0327868852459	GT:AC:AF:NC	0:2:0.0869565217391:T=21,G=2,	0:0:0:T=38,G=1,
+phiX174	1174	.	C	A	0	.	AC=2;AF=0.0327868852459	GT:AC:AF:NC	0:2:0.0869565217391:A=2,C=21,	0:0:0:A=1,C=38,
+phiX174	1178	.	G	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:2:0.0740740740741:C=2,T=1,G=25,	0:0:0:G=38,
+phiX174	1187	.	T	G	0	.	AC=3;AF=0.04	GT:AC:AF:NC	0:3:0.0882352941176:C=1,T=31,G=3,	0:0:0:T=41,
+phiX174	1189	.	T	C	0	.	AC=3;AF=0.0379746835443	GT:AC:AF:NC	0:3:0.0857142857143:C=3,T=32,G=1,	0:0:0:T=44,
+phiX174	1200	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0882352941176:T=31,G=3,	0:0:0:T=48,G=1,
+phiX174	1202	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:0:0:T=32,	0:3:0.06:T=47,G=3,
+phiX174	1205	.	A	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.0588235294118:A=32,C=2,	0:0:0:A=47,C=1,G=1,
+phiX174	1207	.	T	G	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:A=1,T=34,	0:2:0.0425531914894:T=45,G=2,
+phiX174	1214	.	G	A	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:T=1,G=34,	0:2:0.0425531914894:A=2,G=45,
+phiX174	1216	.	C	A	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:C=34,	0:2:0.0425531914894:A=2,C=45,
+phiX174	1217	.	C	A	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.0588235294118:A=2,C=32,	0:0:0:C=46,
+phiX174	1219	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:0:0:C=1,T=33,G=1,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1223	.	C	T	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.0555555555556:C=34,T=2,	0:0:0:C=44,
+phiX174	1227	.	A	C	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0588235294118:A=32,C=2,	0:0:0:A=40,
+phiX174	1232	.	T	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:T=34,	0:2:0.0606060606061:C=1,T=31,G=2,
+phiX174	1236	.	G	T	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0606060606061:A=1,T=2,G=31,	0:0:0:G=35,
+phiX174	1237	.	T	G	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=1,T=33,G=2,	0:0:0:T=33,G=1,
+phiX174	1240	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:0,0:0,0:A=32,T=1,	0:0,2:0,0.0555555555556:A=34,C=2,T=1,
+phiX174	1245	.	G	T	0	.	AC=4;AF=0.0625	GT:AC:AF:NC	0:2:0.0666666666667:C=1,T=2,G=28,	0:2:0.0588235294118:T=2,G=32,
+phiX174	1247	.	A	T	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:2:0.0714285714286:A=26,C=1,T=2,G=1,	0:0:0:A=35,
+phiX174	1250	.	A	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:2:0.0769230769231:A=24,C=2,	0:0:0:A=39,
+phiX174	1251	.	A	G	0	.	AC=3;AF=0.044776119403	GT:AC:AF:NC	0:0:0:A=29,	0:3:0.0789473684211:A=35,C=1,G=3,
+phiX174	1253	.	T	G	0	.	AC=4;AF=0.0526315789474	GT:AC:AF:NC	0:2:0.0645161290323:T=29,G=2,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1257	.	T	G	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:0:0:T=26,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1259	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:A=28,T=1,	0:2:0.0444444444444:A=43,C=2,G=1,
+phiX174	1274	.	G	C	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.0512820512821:C=2,G=37,	0:0:0:G=44,
+phiX174	1277	.	A	C	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=40,G=1,
+phiX174	1279	.	T	G	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0:0:T=39,G=1,	0:2:0.05:C=1,T=38,G=2,
+phiX174	1284	.	T	G,C	0	.	AC=3,2;AF=0.038961038961,0.025974025974	GT:AC:AF:NC	0:3,2:0.075,0.05:C=2,T=35,G=3,	0:0,0:0,0:A=1,T=37,
+phiX174	1289	.	A	C	0	.	AC=3;AF=0.0454545454545	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=28,G=1,
+phiX174	1296	.	A	C	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:0:0:A=38,	0:2:0.0769230769231:A=24,C=2,
+phiX174	1298	.	A	C	0	.	AC=3;AF=0.0491803278689	GT:AC:AF:NC	0:3,0:0.0810810810811,0:A=34,C=3,G=1,	0:0,0:0,0:A=24,C=1,T=1,G=1,
+phiX174	1306	.	T	G	0	.	AC=5;AF=0.0943396226415	GT:AC:AF:NC	0:5:0.178571428571:T=23,G=5,	0:0:0:C=1,T=25,
+phiX174	1307	.	T	G	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.107142857143:T=25,G=3,	0:0:0:C=1,T=27,
+phiX174	1314	.	A	G	0	.	AC=5;AF=0.0877192982456	GT:AC:AF:NC	0:2,0:0.0740740740741,0:A=25,C=1,G=2,	0:3,0:0.1,0:A=27,C=1,G=3,
+phiX174	1317	.	A	C	0	.	AC=2;AF=0.0322580645161	GT:AC:AF:NC	0:2:0.0740740740741:A=25,C=2,	0:0:0:A=35,
+phiX174	1320	.	C	G	0	.	AC=2;AF=0.0322580645161	GT:AC:AF:NC	0:2:0.0769230769231:C=24,G=2,	0:0:0:C=36,
+phiX174	1322	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.107142857143:C=3,G=25,	0:0:0:G=40,
+phiX174	1323	.	C	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:C=28,	0:2:0.0512820512821:C=37,T=1,G=2,
+phiX174	1329	.	T	C	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:2:0.0666666666667:C=2,T=28,	0:0:0:T=42,G=1,
+phiX174	1335	.	C	A	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.0625:A=2,C=30,	0:0:0:C=45,
+phiX174	1338	.	T	A	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:0:0:T=32,	0:2:0.047619047619:A=2,T=40,
+phiX174	1345	.	T	C	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0,0:0,0:T=36,G=1,	0:0,2:0,0.046511627907:C=2,T=41,G=1,
+phiX174	1347	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0512820512821:A=37,C=2,G=1,	0:0:0:A=43,
+phiX174	1348	.	T	G	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.05:T=38,G=2,	0:0:0:T=43,
+phiX174	1352	.	A	T	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:A=34,G=1,	0:2:0.0512820512821:A=37,T=2,
+phiX174	1354	.	T	G,A	0	.	AC=2,2;AF=0.027027027027,0.027027027027	GT:AC:AF:NC	0:2,0:0.0526315789474,0:T=36,G=2,	0:0,2:0,0.0555555555556:A=2,T=34,G=1,
+phiX174	1359	.	T	G	0	.	AC=3;AF=0.0405405405405	GT:AC:AF:NC	0:3:0.0714285714286:T=39,G=3,	0:0:0:T=32,G=1,
+phiX174	1366	.	G	C	0	.	AC=3;AF=0.0428571428571	GT:AC:AF:NC	0:3:0.0714285714286:C=3,T=1,G=39,	0:0:0:G=28,
+phiX174	1370	.	T	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:0:0:C=1,T=45,G=1,	0:2:0.0833333333333:A=1,T=22,G=2,
+phiX174	1373	.	T	G	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0408163265306:T=47,G=2,	0:0:0:T=25,
+phiX174	1380	.	G	C	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:0,2:0,0.0384615384615:C=2,T=1,G=50,	0:0,0:0,0:T=1,G=32,
+phiX174	1381	.	T	G	0	.	AC=3;AF=0.0361445783133	GT:AC:AF:NC	0:3:0.0576923076923:A=1,T=49,G=3,	0:0:0:T=31,
+phiX174	1384	.	T	G	0	.	AC=4;AF=0.0512820512821	GT:AC:AF:NC	0:4:0.0816326530612:T=45,G=4,	0:0:0:T=29,G=1,
+phiX174	1385	.	T	G	0	.	AC=4;AF=0.0512820512821	GT:AC:AF:NC	0:4:0.0816326530612:T=45,G=4,	0:0:0:C=1,T=29,
+phiX174	1391	.	A	C	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:2:0.0408163265306:A=47,C=2,	0:0:0:A=30,C=1,
+phiX174	1393	.	C	A	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:2:0.0425531914894:A=2,C=45,	0:0:0:C=32,
+phiX174	1396	.	T	G	0	.	AC=3;AF=0.04	GT:AC:AF:NC	0:3:0.0697674418605:T=40,G=3,	0:0:0:T=32,
+phiX174	1397	.	A	C	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:0:0:A=42,T=1,	0:2:0.0625:A=30,C=2,
+phiX174	1400	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.047619047619:A=40,C=2,G=1,	0:0:0:A=31,
+phiX174	1401	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.047619047619:A=40,C=2,	0:0:0:A=31,
+phiX174	1434	.	C	T	0	.	AC=2;AF=0.0571428571429	GT:AC:AF:NC	0:2:0.142857142857:C=12,T=2,	0:0:0:C=21,
+phiX174	1438	.	G	C	0	.	AC=2;AF=0.0526315789474	GT:AC:AF:NC	0:0:0:G=15,	0:2:0.0869565217391:C=2,G=21,
+phiX174	1450	.	T	G	0	.	AC=3;AF=0.0789473684211	GT:AC:AF:NC	0:3:0.176470588235:A=1,C=1,T=14,G=3,	0:0:0:T=21,G=1,
+phiX174	1459	.	T	G	0	.	AC=3;AF=0.075	GT:AC:AF:NC	0:3:0.142857142857:C=1,T=18,G=3,	0:0:0:T=19,
+phiX174	1470	.	C	A	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:0:0:C=29,	0:2:0.0869565217391:A=2,C=21,
+phiX174	1484	.	C	G	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:2:0.0740740740741:A=1,C=25,G=2,	0:0:0:C=25,
+phiX174	1487	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0689655172414:T=27,G=2,	0:0:0:T=26,
+phiX174	1491	.	G	T	0	.	AC=2;AF=0.037037037037	GT:AC:AF:NC	0:0:0:T=1,G=26,	0:2:0.0714285714286:T=2,G=26,
+phiX174	1503	.	A	C	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:0:0:A=20,C=1,	0:2:0.0833333333333:A=22,C=2,
+phiX174	1536	.	A	C	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.111111111111:A=24,C=3,	0:0:0:A=28,C=1,
+phiX174	1541	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0740740740741:T=25,G=2,	0:0:0:T=28,
+phiX174	1553	.	A	G	0	.	AC=2;AF=0.0408163265306	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=1,G=2,	0:0:0:A=28,
+phiX174	1566	.	C	G	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0:0:C=30,T=1,	0:2:0.0571428571429:C=33,G=2,
+phiX174	1572	.	T	G	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:2:0.0625:T=30,G=2,	0:0:0:T=32,
+phiX174	1574	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0882352941176:C=3,G=31,	0:0:0:G=34,
+phiX174	1575	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=33,T=1,
+phiX174	1577	.	A	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:0:0:A=35,	0:3:0.0909090909091:A=30,C=3,
+phiX174	1581	.	T	G	0	.	AC=2;AF=0.0285714285714	GT:AC:AF:NC	0:2:0.0555555555556:C=1,T=34,G=2,	0:0:0:T=34,G=1,
+phiX174	1591	.	A	C	0	.	AC=3;AF=0.037037037037	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=43,C=1,
+phiX174	1597	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0606060606061:T=31,G=2,	0:0:0:C=1,T=45,
+phiX174	1603	.	T	G	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0625:T=30,G=2,	0:0:0:T=50,
+phiX174	1606	.	T	G	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0666666666667:A=1,C=1,T=28,G=2,	0:0:0:T=52,
+phiX174	1611	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0769230769231:A=24,C=2,	0:0:0:A=56,C=1,
+phiX174	1613	.	A	G	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:0:0:A=25,C=1,G=1,	0:2:0.0344827586207:A=56,G=2,
+phiX174	1618	.	C	A	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0666666666667:A=2,C=28,G=1,	0:0:0:A=1,C=57,
+phiX174	1619	.	C	A	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0666666666667:A=2,C=28,	0:0:0:C=57,
+phiX174	1621	.	A	C	0	.	AC=3;AF=0.0352941176471	GT:AC:AF:NC	0:0:0:A=31,T=1,G=1,	0:3:0.0555555555556:A=51,C=3,
+phiX174	1622	.	G	T	0	.	AC=2;AF=0.0224719101124	GT:AC:AF:NC	0:0:0:A=1,G=35,	0:2:0.037037037037:T=2,G=52,
+phiX174	1624	.	A	G	0	.	AC=2;AF=0.0235294117647	GT:AC:AF:NC	0:2:0.0555555555556:A=34,T=1,G=2,	0:0:0:A=49,
+phiX174	1627	.	T	G,C	0	.	AC=2,2;AF=0.0246913580247,0.0246913580247	GT:AC:AF:NC	0:0,0:0,0:T=37,G=1,	0:2,2:0.0454545454545,0.0454545454545:C=2,T=40,G=2,
+phiX174	1630	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0526315789474:C=1,T=36,G=2,	0:0:0:T=40,
+phiX174	1632	.	A	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=41,
+phiX174	1634	.	T	G	0	.	AC=4;AF=0.0493827160494	GT:AC:AF:NC	0:2:0.047619047619:C=1,T=40,G=2,	0:2:0.0512820512821:T=37,G=2,
+phiX174	1635	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0714285714286:T=39,G=3,	0:0:0:T=40,
+phiX174	1639	.	G	A	0	.	AC=3;AF=0.0405405405405	GT:AC:AF:NC	0:3:0.075:A=3,T=1,G=37,	0:0:0:G=34,
+phiX174	1645	.	T	G	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0425531914894:T=45,G=2,	0:0:0:T=27,G=1,
+phiX174	1647	.	A	T,G,C	0	.	AC=2,2,2;AF=0.0253164556962,0.0253164556962,0.0253164556962	GT:AC:AF:NC	0:0,2,2:0,0.0384615384615,0.0384615384615:A=48,C=2,G=2,	0:2,0,0:0.0740740740741,0,0:A=25,T=2,
+phiX174	1652	.	G	A	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.037037037037:A=2,G=52,	0:0:0:G=29,
+phiX174	1656	.	T	G	0	.	AC=3;AF=0.0348837209302	GT:AC:AF:NC	0:3:0.0555555555556:T=51,G=3,	0:0:0:T=32,G=1,
+phiX174	1658	.	A	C	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.04:A=48,C=2,	0:0:0:A=33,
+phiX174	1659	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0625:C=1,T=45,G=3,	0:0:0:T=34,
+phiX174	1663	.	T	G	0	.	AC=4;AF=0.0454545454545	GT:AC:AF:NC	0:0:0:A=1,T=53,	0:4:0.114285714286:T=31,G=4,
+phiX174	1665	.	C	A	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0384615384615:A=2,C=50,G=1,	0:0:0:C=42,
+phiX174	1670	.	G	C	0	.	AC=2;AF=0.0224719101124	GT:AC:AF:NC	0:0,2:0,0.0408163265306:A=1,C=2,T=1,G=47,	0:0,0:0,0:T=1,G=40,
+phiX174	1671	.	G	T	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0384615384615:C=1,T=2,G=50,	0:0:0:G=42,
+phiX174	1672	.	A	G	0	.	AC=3;AF=0.0315789473684	GT:AC:AF:NC	0:3:0.0545454545455:A=52,G=3,	0:0:0:A=40,T=1,
+phiX174	1673	.	G	T	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0377358490566:C=1,T=2,G=51,	0:0:0:G=41,
+phiX174	1674	.	G	T	0	.	AC=4;AF=0.04	GT:AC:AF:NC	0:4:0.0714285714286:T=4,G=52,	0:0:0:G=44,
+phiX174	1677	.	A	C	0	.	AC=3;AF=0.031914893617	GT:AC:AF:NC	0:3:0.0566037735849:A=50,C=3,N=1,	0:0:0:A=41,
+phiX174	1678	.	A	C	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:0:0:A=54,C=1,G=1,	0:2:0.05:A=38,C=2,
+phiX174	1682	.	T	C	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0408163265306:C=2,T=47,G=1,	0:0:0:C=1,T=38,
+phiX174	1685	.	T	G	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:2:0.046511627907:A=1,T=41,G=2,	0:0:0:T=41,G=1,
+phiX174	1686	.	A	C	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:0:0:A=39,C=1,T=1,G=1,	0:2:0.0512820512821:A=37,C=2,
+phiX174	1687	.	T	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.047619047619:C=2,T=40,	0:0:0:A=1,T=40,
+phiX174	1688	.	G	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.05:C=2,T=1,G=38,	0:0:0:G=41,
+phiX174	1689	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0487804878049:A=39,C=2,T=1,G=1,	0:0:0:A=41,C=1,
+phiX174	1691	.	G	C	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.05:C=2,T=1,G=38,	0:0:0:G=37,
+phiX174	1693	.	T	G	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:0:0:T=42,G=1,	0:2:0.047619047619:T=40,G=2,
+phiX174	1695	.	A	C	0	.	AC=2;AF=0.0232558139535	GT:AC:AF:NC	0:2:0.046511627907:A=41,C=2,	0:0:0:A=43,C=1,
+phiX174	1702	.	T	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.0512820512821:A=1,C=2,T=37,	0:0:0:C=1,T=42,
+phiX174	1707	.	T	C	0	.	AC=2;AF=0.0232558139535	GT:AC:AF:NC	0:2:0.05:C=2,T=38,	0:0:0:T=46,G=1,
+phiX174	1708	.	A	C	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:2:0.0526315789474:A=36,C=2,	0:0:0:A=46,C=1,G=1,
+phiX174	1710	.	T	C	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:0:0:C=1,T=34,	0:2:0.0434782608696:C=2,T=44,
+phiX174	1723	.	T	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0588235294118:C=2,T=32,	0:0:0:T=48,
+phiX174	1724	.	A	C	0	.	AC=4;AF=0.0481927710843	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:2:0.0416666666667:A=46,C=2,
+phiX174	1725	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0606060606061:A=31,C=2,	0:0:0:A=49,
+phiX174	1726	.	T	G	0	.	AC=3;AF=0.037037037037	GT:AC:AF:NC	0:3:0.0882352941176:T=31,G=3,	0:0:0:C=1,T=47,
+phiX174	1730	.	T	G	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:C=1,T=28,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1739	.	G	T	0	.	AC=3;AF=0.0434782608696	GT:AC:AF:NC	0:3:0.09375:T=3,G=29,	0:0:0:G=37,
+phiX174	1746	.	A	C	0	.	AC=2;AF=0.0338983050847	GT:AC:AF:NC	0:2:0.0689655172414:A=27,C=2,	0:0:0:A=30,C=1,G=1,
+phiX174	1749	.	T	G,C	0	.	AC=3,2;AF=0.044776119403,0.0298507462687	GT:AC:AF:NC	0:3,2:0.0857142857143,0.0571428571429:C=2,T=30,G=3,	0:0,0:0,0:T=32,G=1,
+phiX174	1755	.	G	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.05:C=2,G=38,	0:0:0:C=1,G=33,
+phiX174	1756	.	A	G,C	0	.	AC=2,2;AF=0.0281690140845,0.0281690140845	GT:AC:AF:NC	0:0,2:0,0.0526315789474:A=36,C=2,T=1,G=1,	0:2,0:0.0606060606061,0:A=31,C=1,G=2,
+phiX174	1761	.	A	G	0	.	AC=2;AF=0.0285714285714	GT:AC:AF:NC	0:2:0.0512820512821:A=37,C=1,G=2,	0:0:0:A=31,
+phiX174	1764	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=28,
+phiX174	1766	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0487804878049:A=39,C=2,	0:0:0:A=27,
+phiX174	1769	.	T	G	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:2:0.0526315789474:A=1,C=1,T=36,G=2,	0:0:0:T=26,G=1,
+phiX174	1771	.	G	C	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:2:0.0526315789474:C=2,G=36,	0:0:0:G=29,
+phiX174	1772	.	T	G	0	.	AC=4;AF=0.0597014925373	GT:AC:AF:NC	0:4:0.108108108108:C=1,T=33,G=4,	0:0:0:T=30,
+phiX174	1774	.	A	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0810810810811:A=34,G=3,	0:0:0:A=31,
+phiX174	1775	.	G	A	0	.	AC=2;AF=0.030303030303	GT:AC:AF:NC	0:2:0.0571428571429:A=2,G=33,	0:0:0:G=31,
+phiX174	1776	.	G	T	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0540540540541:T=2,G=35,	0:0:0:G=32,
+phiX174	1778	.	C	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0526315789474:C=36,G=2,	0:0:0:C=31,
+phiX174	1779	.	A	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.075:A=37,C=3,	0:0:0:A=28,
+phiX174	1781	.	T	G	0	.	AC=5;AF=0.0714285714286	GT:AC:AF:NC	0:2:0.0487804878049:C=1,T=39,G=2,	0:3:0.103448275862:T=26,G=3,
+phiX174	1782	.	T	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.075:T=37,G=3,	0:0:0:A=1,T=28,
+phiX174	1783	.	T	C	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:2:0.0512820512821:C=2,T=37,	0:0:0:T=28,G=1,
+phiX174	1788	.	G	T	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:0:0:A=1,T=1,G=29,	0:2:0.0689655172414:T=2,G=27,
+phiX174	1792	.	T	G	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:2:0.0606060606061:T=31,G=2,	0:0:0:T=25,G=1,
+phiX174	1802	.	A	C	0	.	AC=2;AF=0.030303030303	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=31,C=1,
+phiX174	1804	.	C	T	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0588235294118:A=1,C=32,T=2,	0:0:0:C=34,
+phiX174	1805	.	T	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0857142857143:T=32,G=3,	0:0:0:T=33,G=1,
+phiX174	1806	.	A	C	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=34,G=1,
+phiX174	1824	.	G	C	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0714285714286:C=2,T=1,G=26,	0:0:0:G=43,
+phiX174	1825	.	T	G	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0689655172414:T=27,G=2,	0:0:0:T=42,G=1,
+phiX174	1833	.	T	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:0:0:A=1,T=24,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1843	.	T	G	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:2:0.08:A=1,T=23,G=2,	0:0:0:T=38,
+phiX174	1847	.	A	C	0	.	AC=2;AF=0.0333333333333	GT:AC:AF:NC	0:2:0.0833333333333:A=22,C=2,T=1,G=1,	0:0:0:A=36,
+phiX174	1854	.	T	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:T=26,	0:2:0.0487804878049:T=39,G=2,
+phiX174	1860	.	T	G	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:0:0:A=1,T=20,	0:2:0.0606060606061:T=31,G=2,
+phiX174	1863	.	C	G	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=17,G=2,	0:0:0:C=33,
+phiX174	1866	.	T	G	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:0:0:T=19,G=1,	0:2:0.0645161290323:T=29,G=2,
+phiX174	1875	.	T	A	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:2:0.0952380952381:A=2,T=19,	0:0:0:T=32,G=1,
+phiX174	1876	.	T	G	0	.	AC=3;AF=0.0588235294118	GT:AC:AF:NC	0:3:0.157894736842:C=1,T=16,G=3,	0:0:0:T=32,G=1,
+phiX174	1882	.	T	G	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:2:0.105263157895:T=17,G=2,	0:0:0:T=34,
+phiX174	1889	.	A	C	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:2:0.0869565217391:A=21,C=2,	0:0:0:A=27,
+phiX174	1913	.	G	C	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:0:0:G=27,	0:2:0.0869565217391:C=2,G=21,
+phiX174	1915	.	T	G	0	.	AC=2;AF=0.0425531914894	GT:AC:AF:NC	0:0:0:A=1,T=24,	0:2:0.0869565217391:T=21,G=2,
+phiX174	1917	.	A	T	0	.	AC=2;AF=0.0444444444444	GT:AC:AF:NC	0:2,0:0.0833333333333,0:A=22,T=2,G=1,	0:0,0:0,0:A=21,G=1,
+phiX174	1930	.	T	A	0	.	AC=2;AF=0.0526315789474	GT:AC:AF:NC	0:2:0.0909090909091:A=2,T=20,	0:0:0:T=16,
+phiX174	1934	.	A	C	0	.	AC=2;AF=0.0512820512821	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=2,T=1,	0:0:0:A=18,C=1,
+phiX174	1940	.	A	C	0	.	AC=2;AF=0.046511627907	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=2,	0:0:0:A=22,C=1,
+phiX174	1942	.	T	G	0	.	AC=2;AF=0.047619047619	GT:AC:AF:NC	0:0:0:T=19,	0:2:0.0869565217391:T=21,G=2,
+phiX174	1945	.	T	G	0	.	AC=3;AF=0.0769230769231	GT:AC:AF:NC	0:0:0:T=15,G=1,	0:3:0.125:T=21,G=3,
+phiX174	1954	.	T	C	0	.	AC=2;AF=0.0625	GT:AC:AF:NC	0:0,0:0,0:T=12,G=1,	0:0,2:0,0.1:C=2,T=18,G=1,
+phiX174	1959	.	T	G	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:2:0.153846153846:T=11,G=2,	0:0:0:T=23,
+phiX174	1994	.	A	C	0	.	AC=2;AF=0.0512820512821	GT:AC:AF:NC	0:2:0.153846153846:A=11,C=2,	0:0:0:A=26,
+phiX174	1998	.	A	G	0	.	AC=2;AF=0.05	GT:AC:AF:NC	0:2:0.142857142857:A=12,G=2,	0:0:0:A=26,
diff --git a/tool_collections/vcflib/vcfleftalign/test-data/vcflib-phix.vcf b/tool_collections/vcflib/vcfleftalign/test-data/vcflib-phix.vcf
new file mode 100644
index 00000000000..362e6be2088
--- /dev/null
+++ b/tool_collections/vcflib/vcfleftalign/test-data/vcflib-phix.vcf
@@ -0,0 +1,219 @@
+##fileformat=VCFv4.1
+##fileDate=20140610
+##source=Dan
+##reference=file:///galaxy/data/phiX/sam_index/phiX.fa
+##filter="AF > .001"
+##INFO=<ID=AC,Number=A,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=AF,Number=A,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=AF,Number=.,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=NC,Number=.,Type=String,Description="Nucleotide and indel counts">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	groupA	groupB
+phiX174	1015	.	A	C	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:0,2:0,0.2:A=8,C=2,G=1,	0:0,0:0,0:A=26,G=1,
+phiX174	1016	.	A	T	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:0:0:A=10,	0:2:0.0769230769231:A=24,T=2,
+phiX174	1038	.	A	C	0	.	AC=2;AF=0.046511627907	GT:AC:AF:NC	0:2:0.153846153846:A=11,C=2,	0:0:0:A=30,
+phiX174	1045	.	T	G	0	.	AC=2;AF=0.0425531914894	GT:AC:AF:NC	0:0:0:T=15,G=1,	0:2:0.0625:T=30,G=2,
+phiX174	1048	.	C	A	0	.	AC=2;AF=0.0434782608696	GT:AC:AF:NC	0:2:0.133333333333:A=2,C=13,	0:0:0:C=31,G=1,
+phiX174	1050	.	A	C	0	.	AC=3;AF=0.0714285714286	GT:AC:AF:NC	0:0:0:A=12,	0:3:0.1:A=27,C=3,
+phiX174	1051	.	T	G	0	.	AC=2;AF=0.0487804878049	GT:AC:AF:NC	0:2:0.166666666667:C=1,T=10,G=2,	0:0:0:T=29,G=1,
+phiX174	1054	.	T	G	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:2:0.153846153846:T=11,G=2,	0:0:0:T=31,G=1,
+phiX174	1076	.	G	C	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=2,G=17,	0:0:0:G=36,
+phiX174	1080	.	G	C	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=2,T=1,G=17,	0:0:0:C=1,G=39,
+phiX174	1092	.	T	G	0	.	AC=2;AF=0.0263157894737	GT:AC:AF:NC	0:2:0.0833333333333:C=1,T=22,G=2,	0:0:0:T=52,G=1,
+phiX174	1093	.	T	G	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.08:T=23,G=2,	0:0:0:T=52,
+phiX174	1102	.	T	C	0	.	AC=6;AF=0.0779220779221	GT:AC:AF:NC	0:2:0.0869565217391:C=2,T=21,	0:4:0.0740740740741:C=4,T=50,
+phiX174	1107	.	T	G	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:0:0:T=22,G=1,	0:2:0.04:T=48,G=2,
+phiX174	1110	.	T	C	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:2:0.125:C=2,T=14,	0:0:0:C=1,T=56,G=1,
+phiX174	1121	.	T	G	0	.	AC=3;AF=0.041095890411	GT:AC:AF:NC	0:0:0:T=19,	0:3:0.0555555555556:T=51,G=3,
+phiX174	1124	.	T	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0,2:0,0.117647058824:C=2,T=15,G=1,	0:0,0:0,0:T=48,G=1,
+phiX174	1125	.	T	G	0	.	AC=3;AF=0.0454545454545	GT:AC:AF:NC	0:3:0.176470588235:T=14,G=3,	0:0:0:T=49,G=1,
+phiX174	1131	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0952380952381:T=19,G=2,	0:0:0:T=57,
+phiX174	1141	.	T	G	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0:0:T=20,G=1,	0:2:0.0338983050847:T=57,G=2,
+phiX174	1145	.	G	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.0952380952381:C=2,G=19,	0:0:0:G=52,
+phiX174	1147	.	T	C	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0952380952381:C=2,T=19,	0:0:0:T=50,
+phiX174	1149	.	T	G,C	0	.	AC=2,2;AF=0.027027027027,0.027027027027	GT:AC:AF:NC	0:2,2:0.1,0.1:C=2,T=16,G=2,	0:0,0:0,0:T=54,
+phiX174	1156	.	T	G	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:0:0:C=1,T=15,	0:2:0.0416666666667:T=46,G=2,
+phiX174	1159	.	T	C,G	0	.	AC=2,2;AF=0.0298507462687,0.0298507462687	GT:AC:AF:NC	0:2,0:0.111111111111,0:C=2,T=16,	0:0,2:0,0.0408163265306:C=1,T=47,G=2,
+phiX174	1164	.	T	G	0	.	AC=4;AF=0.0666666666667	GT:AC:AF:NC	0:0:0:C=1,T=16,G=1,	0:4:0.0909090909091:T=40,G=4,
+phiX174	1168	.	T	G	0	.	AC=4;AF=0.0666666666667	GT:AC:AF:NC	0:2:0.0952380952381:C=1,T=19,G=2,	0:2:0.0512820512821:T=37,G=2,
+phiX174	1171	.	T	G	0	.	AC=2;AF=0.0327868852459	GT:AC:AF:NC	0:2:0.0869565217391:T=21,G=2,	0:0:0:T=38,G=1,
+phiX174	1174	.	C	A	0	.	AC=2;AF=0.0327868852459	GT:AC:AF:NC	0:2:0.0869565217391:A=2,C=21,	0:0:0:A=1,C=38,
+phiX174	1178	.	G	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:2:0.0740740740741:C=2,T=1,G=25,	0:0:0:G=38,
+phiX174	1187	.	T	G	0	.	AC=3;AF=0.04	GT:AC:AF:NC	0:3:0.0882352941176:C=1,T=31,G=3,	0:0:0:T=41,
+phiX174	1189	.	T	C	0	.	AC=3;AF=0.0379746835443	GT:AC:AF:NC	0:3:0.0857142857143:C=3,T=32,G=1,	0:0:0:T=44,
+phiX174	1200	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0882352941176:T=31,G=3,	0:0:0:T=48,G=1,
+phiX174	1202	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:0:0:T=32,	0:3:0.06:T=47,G=3,
+phiX174	1205	.	A	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.0588235294118:A=32,C=2,	0:0:0:A=47,C=1,G=1,
+phiX174	1207	.	T	G	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:A=1,T=34,	0:2:0.0425531914894:T=45,G=2,
+phiX174	1214	.	G	A	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:T=1,G=34,	0:2:0.0425531914894:A=2,G=45,
+phiX174	1216	.	C	A	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:C=34,	0:2:0.0425531914894:A=2,C=45,
+phiX174	1217	.	C	A	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.0588235294118:A=2,C=32,	0:0:0:C=46,
+phiX174	1219	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:0:0:C=1,T=33,G=1,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1223	.	C	T	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.0555555555556:C=34,T=2,	0:0:0:C=44,
+phiX174	1227	.	A	C	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0588235294118:A=32,C=2,	0:0:0:A=40,
+phiX174	1232	.	T	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:T=34,	0:2:0.0606060606061:C=1,T=31,G=2,
+phiX174	1236	.	G	T	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0606060606061:A=1,T=2,G=31,	0:0:0:G=35,
+phiX174	1237	.	T	G	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=1,T=33,G=2,	0:0:0:T=33,G=1,
+phiX174	1240	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:0,0:0,0:A=32,T=1,	0:0,2:0,0.0555555555556:A=34,C=2,T=1,
+phiX174	1245	.	G	T	0	.	AC=4;AF=0.0625	GT:AC:AF:NC	0:2:0.0666666666667:C=1,T=2,G=28,	0:2:0.0588235294118:T=2,G=32,
+phiX174	1247	.	A	T	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:2:0.0714285714286:A=26,C=1,T=2,G=1,	0:0:0:A=35,
+phiX174	1250	.	A	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:2:0.0769230769231:A=24,C=2,	0:0:0:A=39,
+phiX174	1251	.	A	G	0	.	AC=3;AF=0.044776119403	GT:AC:AF:NC	0:0:0:A=29,	0:3:0.0789473684211:A=35,C=1,G=3,
+phiX174	1253	.	T	G	0	.	AC=4;AF=0.0526315789474	GT:AC:AF:NC	0:2:0.0645161290323:T=29,G=2,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1257	.	T	G	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:0:0:T=26,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1259	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:A=28,T=1,	0:2:0.0444444444444:A=43,C=2,G=1,
+phiX174	1274	.	G	C	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.0512820512821:C=2,G=37,	0:0:0:G=44,
+phiX174	1277	.	A	C	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=40,G=1,
+phiX174	1279	.	T	G	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0:0:T=39,G=1,	0:2:0.05:C=1,T=38,G=2,
+phiX174	1284	.	T	G,C	0	.	AC=3,2;AF=0.038961038961,0.025974025974	GT:AC:AF:NC	0:3,2:0.075,0.05:C=2,T=35,G=3,	0:0,0:0,0:A=1,T=37,
+phiX174	1289	.	A	C	0	.	AC=3;AF=0.0454545454545	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=28,G=1,
+phiX174	1296	.	A	C	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:0:0:A=38,	0:2:0.0769230769231:A=24,C=2,
+phiX174	1298	.	A	C	0	.	AC=3;AF=0.0491803278689	GT:AC:AF:NC	0:3,0:0.0810810810811,0:A=34,C=3,G=1,	0:0,0:0,0:A=24,C=1,T=1,G=1,
+phiX174	1306	.	T	G	0	.	AC=5;AF=0.0943396226415	GT:AC:AF:NC	0:5:0.178571428571:T=23,G=5,	0:0:0:C=1,T=25,
+phiX174	1307	.	T	G	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.107142857143:T=25,G=3,	0:0:0:C=1,T=27,
+phiX174	1314	.	A	G	0	.	AC=5;AF=0.0877192982456	GT:AC:AF:NC	0:2,0:0.0740740740741,0:A=25,C=1,G=2,	0:3,0:0.1,0:A=27,C=1,G=3,
+phiX174	1317	.	A	C	0	.	AC=2;AF=0.0322580645161	GT:AC:AF:NC	0:2:0.0740740740741:A=25,C=2,	0:0:0:A=35,
+phiX174	1320	.	C	G	0	.	AC=2;AF=0.0322580645161	GT:AC:AF:NC	0:2:0.0769230769231:C=24,G=2,	0:0:0:C=36,
+phiX174	1322	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.107142857143:C=3,G=25,	0:0:0:G=40,
+phiX174	1323	.	C	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:C=28,	0:2:0.0512820512821:C=37,T=1,G=2,
+phiX174	1329	.	T	C	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:2:0.0666666666667:C=2,T=28,	0:0:0:T=42,G=1,
+phiX174	1335	.	C	A	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.0625:A=2,C=30,	0:0:0:C=45,
+phiX174	1338	.	T	A	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:0:0:T=32,	0:2:0.047619047619:A=2,T=40,
+phiX174	1345	.	T	C	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0,0:0,0:T=36,G=1,	0:0,2:0,0.046511627907:C=2,T=41,G=1,
+phiX174	1347	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0512820512821:A=37,C=2,G=1,	0:0:0:A=43,
+phiX174	1348	.	T	G	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.05:T=38,G=2,	0:0:0:T=43,
+phiX174	1352	.	A	T	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:A=34,G=1,	0:2:0.0512820512821:A=37,T=2,
+phiX174	1354	.	T	G,A	0	.	AC=2,2;AF=0.027027027027,0.027027027027	GT:AC:AF:NC	0:2,0:0.0526315789474,0:T=36,G=2,	0:0,2:0,0.0555555555556:A=2,T=34,G=1,
+phiX174	1359	.	T	G	0	.	AC=3;AF=0.0405405405405	GT:AC:AF:NC	0:3:0.0714285714286:T=39,G=3,	0:0:0:T=32,G=1,
+phiX174	1366	.	G	C	0	.	AC=3;AF=0.0428571428571	GT:AC:AF:NC	0:3:0.0714285714286:C=3,T=1,G=39,	0:0:0:G=28,
+phiX174	1370	.	T	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:0:0:C=1,T=45,G=1,	0:2:0.0833333333333:A=1,T=22,G=2,
+phiX174	1373	.	T	G	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0408163265306:T=47,G=2,	0:0:0:T=25,
+phiX174	1380	.	G	C	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:0,2:0,0.0384615384615:C=2,T=1,G=50,	0:0,0:0,0:T=1,G=32,
+phiX174	1381	.	T	G	0	.	AC=3;AF=0.0361445783133	GT:AC:AF:NC	0:3:0.0576923076923:A=1,T=49,G=3,	0:0:0:T=31,
+phiX174	1384	.	T	G	0	.	AC=4;AF=0.0512820512821	GT:AC:AF:NC	0:4:0.0816326530612:T=45,G=4,	0:0:0:T=29,G=1,
+phiX174	1385	.	T	G	0	.	AC=4;AF=0.0512820512821	GT:AC:AF:NC	0:4:0.0816326530612:T=45,G=4,	0:0:0:C=1,T=29,
+phiX174	1391	.	A	C	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:2:0.0408163265306:A=47,C=2,	0:0:0:A=30,C=1,
+phiX174	1393	.	C	A	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:2:0.0425531914894:A=2,C=45,	0:0:0:C=32,
+phiX174	1396	.	T	G	0	.	AC=3;AF=0.04	GT:AC:AF:NC	0:3:0.0697674418605:T=40,G=3,	0:0:0:T=32,
+phiX174	1397	.	A	C	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:0:0:A=42,T=1,	0:2:0.0625:A=30,C=2,
+phiX174	1400	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.047619047619:A=40,C=2,G=1,	0:0:0:A=31,
+phiX174	1401	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.047619047619:A=40,C=2,	0:0:0:A=31,
+phiX174	1434	.	C	T	0	.	AC=2;AF=0.0571428571429	GT:AC:AF:NC	0:2:0.142857142857:C=12,T=2,	0:0:0:C=21,
+phiX174	1438	.	G	C	0	.	AC=2;AF=0.0526315789474	GT:AC:AF:NC	0:0:0:G=15,	0:2:0.0869565217391:C=2,G=21,
+phiX174	1450	.	T	G	0	.	AC=3;AF=0.0789473684211	GT:AC:AF:NC	0:3:0.176470588235:A=1,C=1,T=14,G=3,	0:0:0:T=21,G=1,
+phiX174	1459	.	T	G	0	.	AC=3;AF=0.075	GT:AC:AF:NC	0:3:0.142857142857:C=1,T=18,G=3,	0:0:0:T=19,
+phiX174	1470	.	C	A	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:0:0:C=29,	0:2:0.0869565217391:A=2,C=21,
+phiX174	1484	.	C	G	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:2:0.0740740740741:A=1,C=25,G=2,	0:0:0:C=25,
+phiX174	1487	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0689655172414:T=27,G=2,	0:0:0:T=26,
+phiX174	1491	.	G	T	0	.	AC=2;AF=0.037037037037	GT:AC:AF:NC	0:0:0:T=1,G=26,	0:2:0.0714285714286:T=2,G=26,
+phiX174	1503	.	A	C	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:0:0:A=20,C=1,	0:2:0.0833333333333:A=22,C=2,
+phiX174	1536	.	A	C	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.111111111111:A=24,C=3,	0:0:0:A=28,C=1,
+phiX174	1541	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0740740740741:T=25,G=2,	0:0:0:T=28,
+phiX174	1553	.	A	G	0	.	AC=2;AF=0.0408163265306	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=1,G=2,	0:0:0:A=28,
+phiX174	1566	.	C	G	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0:0:C=30,T=1,	0:2:0.0571428571429:C=33,G=2,
+phiX174	1572	.	T	G	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:2:0.0625:T=30,G=2,	0:0:0:T=32,
+phiX174	1574	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0882352941176:C=3,G=31,	0:0:0:G=34,
+phiX174	1575	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=33,T=1,
+phiX174	1577	.	A	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:0:0:A=35,	0:3:0.0909090909091:A=30,C=3,
+phiX174	1581	.	T	G	0	.	AC=2;AF=0.0285714285714	GT:AC:AF:NC	0:2:0.0555555555556:C=1,T=34,G=2,	0:0:0:T=34,G=1,
+phiX174	1591	.	A	C	0	.	AC=3;AF=0.037037037037	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=43,C=1,
+phiX174	1597	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0606060606061:T=31,G=2,	0:0:0:C=1,T=45,
+phiX174	1603	.	T	G	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0625:T=30,G=2,	0:0:0:T=50,
+phiX174	1606	.	T	G	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0666666666667:A=1,C=1,T=28,G=2,	0:0:0:T=52,
+phiX174	1611	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0769230769231:A=24,C=2,	0:0:0:A=56,C=1,
+phiX174	1613	.	A	G	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:0:0:A=25,C=1,G=1,	0:2:0.0344827586207:A=56,G=2,
+phiX174	1618	.	C	A	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0666666666667:A=2,C=28,G=1,	0:0:0:A=1,C=57,
+phiX174	1619	.	C	A	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0666666666667:A=2,C=28,	0:0:0:C=57,
+phiX174	1621	.	A	C	0	.	AC=3;AF=0.0352941176471	GT:AC:AF:NC	0:0:0:A=31,T=1,G=1,	0:3:0.0555555555556:A=51,C=3,
+phiX174	1622	.	G	T	0	.	AC=2;AF=0.0224719101124	GT:AC:AF:NC	0:0:0:A=1,G=35,	0:2:0.037037037037:T=2,G=52,
+phiX174	1624	.	A	G	0	.	AC=2;AF=0.0235294117647	GT:AC:AF:NC	0:2:0.0555555555556:A=34,T=1,G=2,	0:0:0:A=49,
+phiX174	1627	.	T	G,C	0	.	AC=2,2;AF=0.0246913580247,0.0246913580247	GT:AC:AF:NC	0:0,0:0,0:T=37,G=1,	0:2,2:0.0454545454545,0.0454545454545:C=2,T=40,G=2,
+phiX174	1630	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0526315789474:C=1,T=36,G=2,	0:0:0:T=40,
+phiX174	1632	.	A	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=41,
+phiX174	1634	.	T	G	0	.	AC=4;AF=0.0493827160494	GT:AC:AF:NC	0:2:0.047619047619:C=1,T=40,G=2,	0:2:0.0512820512821:T=37,G=2,
+phiX174	1635	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0714285714286:T=39,G=3,	0:0:0:T=40,
+phiX174	1639	.	G	A	0	.	AC=3;AF=0.0405405405405	GT:AC:AF:NC	0:3:0.075:A=3,T=1,G=37,	0:0:0:G=34,
+phiX174	1645	.	T	G	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0425531914894:T=45,G=2,	0:0:0:T=27,G=1,
+phiX174	1647	.	A	T,G,C	0	.	AC=2,2,2;AF=0.0253164556962,0.0253164556962,0.0253164556962	GT:AC:AF:NC	0:0,2,2:0,0.0384615384615,0.0384615384615:A=48,C=2,G=2,	0:2,0,0:0.0740740740741,0,0:A=25,T=2,
+phiX174	1652	.	G	A	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.037037037037:A=2,G=52,	0:0:0:G=29,
+phiX174	1656	.	T	G	0	.	AC=3;AF=0.0348837209302	GT:AC:AF:NC	0:3:0.0555555555556:T=51,G=3,	0:0:0:T=32,G=1,
+phiX174	1658	.	A	C	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.04:A=48,C=2,	0:0:0:A=33,
+phiX174	1659	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0625:C=1,T=45,G=3,	0:0:0:T=34,
+phiX174	1663	.	T	G	0	.	AC=4;AF=0.0454545454545	GT:AC:AF:NC	0:0:0:A=1,T=53,	0:4:0.114285714286:T=31,G=4,
+phiX174	1665	.	C	A	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0384615384615:A=2,C=50,G=1,	0:0:0:C=42,
+phiX174	1670	.	G	C	0	.	AC=2;AF=0.0224719101124	GT:AC:AF:NC	0:0,2:0,0.0408163265306:A=1,C=2,T=1,G=47,	0:0,0:0,0:T=1,G=40,
+phiX174	1671	.	G	T	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0384615384615:C=1,T=2,G=50,	0:0:0:G=42,
+phiX174	1672	.	A	G	0	.	AC=3;AF=0.0315789473684	GT:AC:AF:NC	0:3:0.0545454545455:A=52,G=3,	0:0:0:A=40,T=1,
+phiX174	1673	.	G	T	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0377358490566:C=1,T=2,G=51,	0:0:0:G=41,
+phiX174	1674	.	G	T	0	.	AC=4;AF=0.04	GT:AC:AF:NC	0:4:0.0714285714286:T=4,G=52,	0:0:0:G=44,
+phiX174	1677	.	A	C	0	.	AC=3;AF=0.031914893617	GT:AC:AF:NC	0:3:0.0566037735849:A=50,C=3,N=1,	0:0:0:A=41,
+phiX174	1678	.	A	C	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:0:0:A=54,C=1,G=1,	0:2:0.05:A=38,C=2,
+phiX174	1682	.	T	C	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0408163265306:C=2,T=47,G=1,	0:0:0:C=1,T=38,
+phiX174	1685	.	T	G	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:2:0.046511627907:A=1,T=41,G=2,	0:0:0:T=41,G=1,
+phiX174	1686	.	A	C	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:0:0:A=39,C=1,T=1,G=1,	0:2:0.0512820512821:A=37,C=2,
+phiX174	1687	.	T	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.047619047619:C=2,T=40,	0:0:0:A=1,T=40,
+phiX174	1688	.	G	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.05:C=2,T=1,G=38,	0:0:0:G=41,
+phiX174	1689	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0487804878049:A=39,C=2,T=1,G=1,	0:0:0:A=41,C=1,
+phiX174	1691	.	G	C	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.05:C=2,T=1,G=38,	0:0:0:G=37,
+phiX174	1693	.	T	G	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:0:0:T=42,G=1,	0:2:0.047619047619:T=40,G=2,
+phiX174	1695	.	A	C	0	.	AC=2;AF=0.0232558139535	GT:AC:AF:NC	0:2:0.046511627907:A=41,C=2,	0:0:0:A=43,C=1,
+phiX174	1702	.	T	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.0512820512821:A=1,C=2,T=37,	0:0:0:C=1,T=42,
+phiX174	1707	.	T	C	0	.	AC=2;AF=0.0232558139535	GT:AC:AF:NC	0:2:0.05:C=2,T=38,	0:0:0:T=46,G=1,
+phiX174	1708	.	A	C	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:2:0.0526315789474:A=36,C=2,	0:0:0:A=46,C=1,G=1,
+phiX174	1710	.	T	C	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:0:0:C=1,T=34,	0:2:0.0434782608696:C=2,T=44,
+phiX174	1723	.	T	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0588235294118:C=2,T=32,	0:0:0:T=48,
+phiX174	1724	.	A	C	0	.	AC=4;AF=0.0481927710843	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:2:0.0416666666667:A=46,C=2,
+phiX174	1725	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0606060606061:A=31,C=2,	0:0:0:A=49,
+phiX174	1726	.	T	G	0	.	AC=3;AF=0.037037037037	GT:AC:AF:NC	0:3:0.0882352941176:T=31,G=3,	0:0:0:C=1,T=47,
+phiX174	1730	.	T	G	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:C=1,T=28,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1739	.	G	T	0	.	AC=3;AF=0.0434782608696	GT:AC:AF:NC	0:3:0.09375:T=3,G=29,	0:0:0:G=37,
+phiX174	1746	.	A	C	0	.	AC=2;AF=0.0338983050847	GT:AC:AF:NC	0:2:0.0689655172414:A=27,C=2,	0:0:0:A=30,C=1,G=1,
+phiX174	1749	.	T	G,C	0	.	AC=3,2;AF=0.044776119403,0.0298507462687	GT:AC:AF:NC	0:3,2:0.0857142857143,0.0571428571429:C=2,T=30,G=3,	0:0,0:0,0:T=32,G=1,
+phiX174	1755	.	G	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.05:C=2,G=38,	0:0:0:C=1,G=33,
+phiX174	1756	.	A	G,C	0	.	AC=2,2;AF=0.0281690140845,0.0281690140845	GT:AC:AF:NC	0:0,2:0,0.0526315789474:A=36,C=2,T=1,G=1,	0:2,0:0.0606060606061,0:A=31,C=1,G=2,
+phiX174	1761	.	A	G	0	.	AC=2;AF=0.0285714285714	GT:AC:AF:NC	0:2:0.0512820512821:A=37,C=1,G=2,	0:0:0:A=31,
+phiX174	1764	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=28,
+phiX174	1766	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0487804878049:A=39,C=2,	0:0:0:A=27,
+phiX174	1769	.	T	G	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:2:0.0526315789474:A=1,C=1,T=36,G=2,	0:0:0:T=26,G=1,
+phiX174	1771	.	G	C	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:2:0.0526315789474:C=2,G=36,	0:0:0:G=29,
+phiX174	1772	.	T	G	0	.	AC=4;AF=0.0597014925373	GT:AC:AF:NC	0:4:0.108108108108:C=1,T=33,G=4,	0:0:0:T=30,
+phiX174	1774	.	A	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0810810810811:A=34,G=3,	0:0:0:A=31,
+phiX174	1775	.	G	A	0	.	AC=2;AF=0.030303030303	GT:AC:AF:NC	0:2:0.0571428571429:A=2,G=33,	0:0:0:G=31,
+phiX174	1776	.	G	T	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0540540540541:T=2,G=35,	0:0:0:G=32,
+phiX174	1778	.	C	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0526315789474:C=36,G=2,	0:0:0:C=31,
+phiX174	1779	.	A	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.075:A=37,C=3,	0:0:0:A=28,
+phiX174	1781	.	T	G	0	.	AC=5;AF=0.0714285714286	GT:AC:AF:NC	0:2:0.0487804878049:C=1,T=39,G=2,	0:3:0.103448275862:T=26,G=3,
+phiX174	1782	.	T	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.075:T=37,G=3,	0:0:0:A=1,T=28,
+phiX174	1783	.	T	C	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:2:0.0512820512821:C=2,T=37,	0:0:0:T=28,G=1,
+phiX174	1788	.	G	T	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:0:0:A=1,T=1,G=29,	0:2:0.0689655172414:T=2,G=27,
+phiX174	1792	.	T	G	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:2:0.0606060606061:T=31,G=2,	0:0:0:T=25,G=1,
+phiX174	1802	.	A	C	0	.	AC=2;AF=0.030303030303	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=31,C=1,
+phiX174	1804	.	C	T	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0588235294118:A=1,C=32,T=2,	0:0:0:C=34,
+phiX174	1805	.	T	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0857142857143:T=32,G=3,	0:0:0:T=33,G=1,
+phiX174	1806	.	A	C	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=34,G=1,
+phiX174	1824	.	G	C	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0714285714286:C=2,T=1,G=26,	0:0:0:G=43,
+phiX174	1825	.	T	G	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0689655172414:T=27,G=2,	0:0:0:T=42,G=1,
+phiX174	1833	.	T	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:0:0:A=1,T=24,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1843	.	T	G	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:2:0.08:A=1,T=23,G=2,	0:0:0:T=38,
+phiX174	1847	.	A	C	0	.	AC=2;AF=0.0333333333333	GT:AC:AF:NC	0:2:0.0833333333333:A=22,C=2,T=1,G=1,	0:0:0:A=36,
+phiX174	1854	.	T	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:T=26,	0:2:0.0487804878049:T=39,G=2,
+phiX174	1860	.	T	G	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:0:0:A=1,T=20,	0:2:0.0606060606061:T=31,G=2,
+phiX174	1863	.	C	G	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=17,G=2,	0:0:0:C=33,
+phiX174	1866	.	T	G	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:0:0:T=19,G=1,	0:2:0.0645161290323:T=29,G=2,
+phiX174	1875	.	T	A	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:2:0.0952380952381:A=2,T=19,	0:0:0:T=32,G=1,
+phiX174	1876	.	T	G	0	.	AC=3;AF=0.0588235294118	GT:AC:AF:NC	0:3:0.157894736842:C=1,T=16,G=3,	0:0:0:T=32,G=1,
+phiX174	1882	.	T	G	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:2:0.105263157895:T=17,G=2,	0:0:0:T=34,
+phiX174	1889	.	A	C	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:2:0.0869565217391:A=21,C=2,	0:0:0:A=27,
+phiX174	1913	.	G	C	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:0:0:G=27,	0:2:0.0869565217391:C=2,G=21,
+phiX174	1915	.	T	G	0	.	AC=2;AF=0.0425531914894	GT:AC:AF:NC	0:0:0:A=1,T=24,	0:2:0.0869565217391:T=21,G=2,
+phiX174	1917	.	A	T	0	.	AC=2;AF=0.0444444444444	GT:AC:AF:NC	0:2,0:0.0833333333333,0:A=22,T=2,G=1,	0:0,0:0,0:A=21,G=1,
+phiX174	1930	.	T	A	0	.	AC=2;AF=0.0526315789474	GT:AC:AF:NC	0:2:0.0909090909091:A=2,T=20,	0:0:0:T=16,
+phiX174	1934	.	A	C	0	.	AC=2;AF=0.0512820512821	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=2,T=1,	0:0:0:A=18,C=1,
+phiX174	1940	.	A	C	0	.	AC=2;AF=0.046511627907	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=2,	0:0:0:A=22,C=1,
+phiX174	1942	.	T	G	0	.	AC=2;AF=0.047619047619	GT:AC:AF:NC	0:0:0:T=19,	0:2:0.0869565217391:T=21,G=2,
+phiX174	1945	.	T	G	0	.	AC=3;AF=0.0769230769231	GT:AC:AF:NC	0:0:0:T=15,G=1,	0:3:0.125:T=21,G=3,
+phiX174	1954	.	T	C	0	.	AC=2;AF=0.0625	GT:AC:AF:NC	0:0,0:0,0:T=12,G=1,	0:0,2:0,0.1:C=2,T=18,G=1,
+phiX174	1959	.	T	G	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:2:0.153846153846:T=11,G=2,	0:0:0:T=23,
+phiX174	1994	.	A	C	0	.	AC=2;AF=0.0512820512821	GT:AC:AF:NC	0:2:0.153846153846:A=11,C=2,	0:0:0:A=26,
+phiX174	1998	.	A	G	0	.	AC=2;AF=0.05	GT:AC:AF:NC	0:2:0.142857142857:A=12,G=2,	0:0:0:A=26,
diff --git a/tool_collections/vcflib/vcfleftalign/test-data/vcflib-test-genome-phix.fa b/tool_collections/vcflib/vcfleftalign/test-data/vcflib-test-genome-phix.fa
new file mode 100644
index 00000000000..48a6b46678a
--- /dev/null
+++ b/tool_collections/vcflib/vcfleftalign/test-data/vcflib-test-genome-phix.fa
@@ -0,0 +1,2 @@
+>phiX174
+GAGTTTTATCGCTTCCATGACGCAGAAGTTAACACTTTCGGATATTTCTGATGAGTCGAAAAATTATCTTGATAAAGCAGGAATTACTACTGCTTGTTTACGAATTAAATCGAAGTGGACTGCTGGCGGAAAATGAGAAAATTCGACCTATCCTTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGTTTAGATATGAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTGGATTACTATCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAGAAATCATGAGTCAAGTTACTGAACAATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTAACCGAAGATGATTTCGATTTTCTGACGAGTAACAAAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCTTGCGTTTATGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTTTATTGCTGCCGTCATTGCTTATTATGTTCATCCCGTCAACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTACGGAAAACATTATTAATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAACACTGACGTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAAATTACGTGCaGAAGGAGTGATGTAATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATTTCAACTACTCCGGTTATCGCTGGCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTTTTTATGTCCCTCATCGTCACGTTTATGGTGAACAGTGGATTAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCCGACTGTTAACACTACTGGTTATATTGACCATGCCGCTTTTCTTGGCACGATTAACCCTGATACCAATAAAATCCCTAAGCATTTGTTTCAGGGTTATTTGAATATCTATAACAACTATTTTAAAGCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATCAAGATGATGCTCGTTATGGTTTCCGTTGCTGCCATCTCAAAAACATTTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGACTTCTACCACATCTATTGACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGCATACTGACCAAGAACGTGATTACTTCATGCAGCGTTACCgTGATGTTATTTCTTCATTTGGAGGTAAAACCTCTTATGACGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCTATGATGTTGATGGAACTGACCAAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAACATTCTGTGCCGCGTTTCTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGCGACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGCTTTGACTTATACCGATATTGCTGGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTGCTGAGGGTCAGTGGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGATTATGACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGAATAGTCAGGTTAAATTTAATGTGACCGTTTATCGCAATCTGCCGACCACTCGCGATTCAATCATGACTTCGTGATAAAAGATTGAGTGTGAGGTTATAACGCCGAAGCGGTAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACCAAGCGAAGCGCGGTAGGTTTTCTGCTTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTCGCCATAATTCAAACTTTTTTTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTTTACAGACACCTAAAGCTACATCGTCAACGTTATATTTTGATAGTTTGACGGTTAATGCTGGTAATGGTGGTTTTCTTCATTGCATTCAGATGGATACATCTGTCAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGCCGACCCTAAATTTTTTGCCTGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTCCCGACTGCCTATGATGTTTATCCTTTGAATGGTCGCCATGATGGTGGTTATTATACCGTCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTACGCCGGGCAATAAtGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGGTGATTTATGTTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAAAGCCGCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACCGATAACAATACTGTAGGCATGGGTGATGCTGGTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCAACTGGACAATCAGAAAGAGATTGCCGAGATGCAAAATGAGACTCAAAAAGAGATTGCTGGCATTCAGTCGGCGACTTCACGCCAGAATACGAAAGACCAGGTATATGCACAAAATGAGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGCGTTGCGTCTATTATGGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAAGCTCAAACGGCTGGTCAGTATTTTACCAATGACCAAATCAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCTCTTCTCATATTGGCGCTACTGCAAAGGATATTTCTAATGTCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTTTTCATGGTATTGATAAAGCTGTTGCCGATACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTCTAATTTGTCTAGGAAATAACCGTCAGGATTGACACCCTCCCAATTGTATGTTTTCATGCCTCCAAATCTTGGAGGCTTTTTTATGGTTCGTTCTTATTACCCTTCTGAATGTCACGCTGATTATTTTGACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAATGCTTGGCTTCCATAAGCAGATGGATAACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAGGGCGTTGAGTTCGATAATGGTGATATGTATGTTGACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTACTGAGAAGTTAATGGATGAATTGGCACAATGCTACAATGTGCTCCCCCAACTTGATATTAATAACACTATAGACCACCGCCCCGAAGGGGACGAAAAATGGTTTTTAGAGAACGAGAAGACGGTTACGCAGTTTTGCCGCAAGCTGGCTGCTGAACGCCCTCTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTaCTATTCAGCGTTTGATGAATGCAATGCGACAGGCTCATGCTGATGGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCTGACGACCGATTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGTGACTATTTTCGTGATATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATGATTCACACGCCGACTGCTATCAGTATTTTTGTGTGCCTGAGTATGGTACAGCTAATGGCCGTCTTCATTTCCATGCGGTGCAtTTTATGCGGACACTTCCTACAGGTAGCGTTGACCCTAATTTTGGTCGTCGGGTACGCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGTGGCCTTATGGTTACAGTATGCCCATCGCAGTTCGCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTAAATACGTTAACAAAAAGTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCACTAAAAACCAAGCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAGAATGAGCCGCAACTTCGGGATGAAAATGCTCACAATGACAAATCTGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGATATTGAAGCAGAACGCAAAAAGAGAGATGAGATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTTTGGCGGCGCAACCTGTGACGACAAATCTGCTCAAATTTATGCGCGCTTCGATAAAAATGATTGGCGTATCCAACCTGCA
diff --git a/tool_collections/vcflib/vcfleftalign/tool-data/fasta_indexes.loc.sample b/tool_collections/vcflib/vcfleftalign/tool-data/fasta_indexes.loc.sample
new file mode 120000
index 00000000000..6d7aec6c449
--- /dev/null
+++ b/tool_collections/vcflib/vcfleftalign/tool-data/fasta_indexes.loc.sample
@@ -0,0 +1 @@
+../../tool-data/fasta_indexes.loc.sample
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfleftalign/tool-data/tool_data_table_conf.xml.sample b/tool_collections/vcflib/vcfleftalign/tool-data/tool_data_table_conf.xml.sample
new file mode 120000
index 00000000000..7bfcf015ca6
--- /dev/null
+++ b/tool_collections/vcflib/vcfleftalign/tool-data/tool_data_table_conf.xml.sample
@@ -0,0 +1 @@
+../../tool-data/tool_data_table_conf.xml.sample
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfleftalign/vcfleftalign.xml b/tool_collections/vcflib/vcfleftalign/vcfleftalign.xml
new file mode 100644
index 00000000000..c3f968d987f
--- /dev/null
+++ b/tool_collections/vcflib/vcfleftalign/vcfleftalign.xml
@@ -0,0 +1,57 @@
+<tool id="vcfleftalign" name="VCFleftAlign:" version="@WRAPPER_VERSION@.0">
+  <description>Left-align indels and complex variants in VCF dataset</description>
+  <macros>
+    <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"/>
+  <expand macro="stdio" />
+  <command>
+    #set $reference_fasta_filename = "localref.fa"
+    #if str( $reference_source.reference_source_selector ) == "history":
+       ln -s "${reference_source.ref_file}" "${reference_fasta_filename}" &amp;&amp;
+    #else:
+       #set $reference_fasta_filename = str( $reference_source.ref_file.fields.path )
+    #end if    
+   vcfleftalign -r "${reference_fasta_filename}" -w "${window_size}" "${input_vcf}" > "${out_file1}"</command>
+  <inputs>
+<param name="input_vcf" type="data" format="vcf" label="VCF dataset to left-align" />
+    <conditional name="reference_source">
+       <param name="reference_source_selector" type="select" label="Choose the source for the reference genome">
+         <option value="cached">Locally cached</option>
+         <option value="history">History</option>
+       </param>
+       <when value="cached">
+         <param name="ref_file" type="select" label="Select reference genome">
+           <options from_data_table="fasta_indexes">
+           </options>
+	   <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/>
+         </param>
+       </when>
+       <when value="history"> <!-- FIX ME!!!! -->
+         <param name="ref_file" type="data" format="fasta" label="Using reference file" />
+       </when>
+     </conditional>
+     <param name="window_size" type="integer" value="150" label="Use a window of this many bp when left-aligning" help="default = 150 bp" />
+  </inputs>
+  <outputs>
+    <data format="vcf" name="out_file1" />
+  </outputs>
+  <tests>
+    <test>
+      <param name="reference_source_selector" value="history" />
+      <param name="input_vcf" value="vcflib-phix.vcf"/>
+      <param name="ref_file" value="vcflib-test-genome-phix.fa" />
+      <param name="window_size" value="150" />
+      <output name="out_file1" file="vcfleftalign-test1.vcf"/>
+    </test>
+    </tests>
+  <help>
+
+Left-aligns variants in VCF dataset.  Window size is determined dynamically according to the entropy of the regions flanking the indel.  These must have entropy > 1 bit/bp, or be shorter than ~5kb.
+
+----
+
+Vcfleftalign @IS_PART_OF_VCFLIB@
+</help>
+  <expand macro="citations" />
+</tool>
diff --git a/tool_collections/vcflib/vcfprimers/.shed.yml b/tool_collections/vcflib/vcfprimers/.shed.yml
new file mode 100644
index 00000000000..4e273fc06a7
--- /dev/null
+++ b/tool_collections/vcflib/vcfprimers/.shed.yml
@@ -0,0 +1,12 @@
+categories:
+- Variant Analysis
+description: Extract flanking sequences for each VCF record
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  For each VCF record, extract the flanking sequences, and write them to stdout as FASTA
+  records suitable for alignment.  This tool is intended for use in designing validation
+  experiments.  Primers extracted which would flank all of the alleles at multi-allelic sites.
+name: vcfprimers
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfprimers
+type: unrestricted
diff --git a/tool_collections/vcflib/vcfprimers/macros.xml b/tool_collections/vcflib/vcfprimers/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcfprimers/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfprimers/test-data/vcflib-phix.vcf b/tool_collections/vcflib/vcfprimers/test-data/vcflib-phix.vcf
new file mode 100644
index 00000000000..362e6be2088
--- /dev/null
+++ b/tool_collections/vcflib/vcfprimers/test-data/vcflib-phix.vcf
@@ -0,0 +1,219 @@
+##fileformat=VCFv4.1
+##fileDate=20140610
+##source=Dan
+##reference=file:///galaxy/data/phiX/sam_index/phiX.fa
+##filter="AF > .001"
+##INFO=<ID=AC,Number=A,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=AF,Number=A,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=AF,Number=.,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=NC,Number=.,Type=String,Description="Nucleotide and indel counts">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	groupA	groupB
+phiX174	1015	.	A	C	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:0,2:0,0.2:A=8,C=2,G=1,	0:0,0:0,0:A=26,G=1,
+phiX174	1016	.	A	T	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:0:0:A=10,	0:2:0.0769230769231:A=24,T=2,
+phiX174	1038	.	A	C	0	.	AC=2;AF=0.046511627907	GT:AC:AF:NC	0:2:0.153846153846:A=11,C=2,	0:0:0:A=30,
+phiX174	1045	.	T	G	0	.	AC=2;AF=0.0425531914894	GT:AC:AF:NC	0:0:0:T=15,G=1,	0:2:0.0625:T=30,G=2,
+phiX174	1048	.	C	A	0	.	AC=2;AF=0.0434782608696	GT:AC:AF:NC	0:2:0.133333333333:A=2,C=13,	0:0:0:C=31,G=1,
+phiX174	1050	.	A	C	0	.	AC=3;AF=0.0714285714286	GT:AC:AF:NC	0:0:0:A=12,	0:3:0.1:A=27,C=3,
+phiX174	1051	.	T	G	0	.	AC=2;AF=0.0487804878049	GT:AC:AF:NC	0:2:0.166666666667:C=1,T=10,G=2,	0:0:0:T=29,G=1,
+phiX174	1054	.	T	G	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:2:0.153846153846:T=11,G=2,	0:0:0:T=31,G=1,
+phiX174	1076	.	G	C	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=2,G=17,	0:0:0:G=36,
+phiX174	1080	.	G	C	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=2,T=1,G=17,	0:0:0:C=1,G=39,
+phiX174	1092	.	T	G	0	.	AC=2;AF=0.0263157894737	GT:AC:AF:NC	0:2:0.0833333333333:C=1,T=22,G=2,	0:0:0:T=52,G=1,
+phiX174	1093	.	T	G	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.08:T=23,G=2,	0:0:0:T=52,
+phiX174	1102	.	T	C	0	.	AC=6;AF=0.0779220779221	GT:AC:AF:NC	0:2:0.0869565217391:C=2,T=21,	0:4:0.0740740740741:C=4,T=50,
+phiX174	1107	.	T	G	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:0:0:T=22,G=1,	0:2:0.04:T=48,G=2,
+phiX174	1110	.	T	C	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:2:0.125:C=2,T=14,	0:0:0:C=1,T=56,G=1,
+phiX174	1121	.	T	G	0	.	AC=3;AF=0.041095890411	GT:AC:AF:NC	0:0:0:T=19,	0:3:0.0555555555556:T=51,G=3,
+phiX174	1124	.	T	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0,2:0,0.117647058824:C=2,T=15,G=1,	0:0,0:0,0:T=48,G=1,
+phiX174	1125	.	T	G	0	.	AC=3;AF=0.0454545454545	GT:AC:AF:NC	0:3:0.176470588235:T=14,G=3,	0:0:0:T=49,G=1,
+phiX174	1131	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0952380952381:T=19,G=2,	0:0:0:T=57,
+phiX174	1141	.	T	G	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0:0:T=20,G=1,	0:2:0.0338983050847:T=57,G=2,
+phiX174	1145	.	G	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.0952380952381:C=2,G=19,	0:0:0:G=52,
+phiX174	1147	.	T	C	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0952380952381:C=2,T=19,	0:0:0:T=50,
+phiX174	1149	.	T	G,C	0	.	AC=2,2;AF=0.027027027027,0.027027027027	GT:AC:AF:NC	0:2,2:0.1,0.1:C=2,T=16,G=2,	0:0,0:0,0:T=54,
+phiX174	1156	.	T	G	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:0:0:C=1,T=15,	0:2:0.0416666666667:T=46,G=2,
+phiX174	1159	.	T	C,G	0	.	AC=2,2;AF=0.0298507462687,0.0298507462687	GT:AC:AF:NC	0:2,0:0.111111111111,0:C=2,T=16,	0:0,2:0,0.0408163265306:C=1,T=47,G=2,
+phiX174	1164	.	T	G	0	.	AC=4;AF=0.0666666666667	GT:AC:AF:NC	0:0:0:C=1,T=16,G=1,	0:4:0.0909090909091:T=40,G=4,
+phiX174	1168	.	T	G	0	.	AC=4;AF=0.0666666666667	GT:AC:AF:NC	0:2:0.0952380952381:C=1,T=19,G=2,	0:2:0.0512820512821:T=37,G=2,
+phiX174	1171	.	T	G	0	.	AC=2;AF=0.0327868852459	GT:AC:AF:NC	0:2:0.0869565217391:T=21,G=2,	0:0:0:T=38,G=1,
+phiX174	1174	.	C	A	0	.	AC=2;AF=0.0327868852459	GT:AC:AF:NC	0:2:0.0869565217391:A=2,C=21,	0:0:0:A=1,C=38,
+phiX174	1178	.	G	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:2:0.0740740740741:C=2,T=1,G=25,	0:0:0:G=38,
+phiX174	1187	.	T	G	0	.	AC=3;AF=0.04	GT:AC:AF:NC	0:3:0.0882352941176:C=1,T=31,G=3,	0:0:0:T=41,
+phiX174	1189	.	T	C	0	.	AC=3;AF=0.0379746835443	GT:AC:AF:NC	0:3:0.0857142857143:C=3,T=32,G=1,	0:0:0:T=44,
+phiX174	1200	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0882352941176:T=31,G=3,	0:0:0:T=48,G=1,
+phiX174	1202	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:0:0:T=32,	0:3:0.06:T=47,G=3,
+phiX174	1205	.	A	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.0588235294118:A=32,C=2,	0:0:0:A=47,C=1,G=1,
+phiX174	1207	.	T	G	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:A=1,T=34,	0:2:0.0425531914894:T=45,G=2,
+phiX174	1214	.	G	A	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:T=1,G=34,	0:2:0.0425531914894:A=2,G=45,
+phiX174	1216	.	C	A	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:C=34,	0:2:0.0425531914894:A=2,C=45,
+phiX174	1217	.	C	A	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.0588235294118:A=2,C=32,	0:0:0:C=46,
+phiX174	1219	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:0:0:C=1,T=33,G=1,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1223	.	C	T	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.0555555555556:C=34,T=2,	0:0:0:C=44,
+phiX174	1227	.	A	C	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0588235294118:A=32,C=2,	0:0:0:A=40,
+phiX174	1232	.	T	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:T=34,	0:2:0.0606060606061:C=1,T=31,G=2,
+phiX174	1236	.	G	T	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0606060606061:A=1,T=2,G=31,	0:0:0:G=35,
+phiX174	1237	.	T	G	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=1,T=33,G=2,	0:0:0:T=33,G=1,
+phiX174	1240	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:0,0:0,0:A=32,T=1,	0:0,2:0,0.0555555555556:A=34,C=2,T=1,
+phiX174	1245	.	G	T	0	.	AC=4;AF=0.0625	GT:AC:AF:NC	0:2:0.0666666666667:C=1,T=2,G=28,	0:2:0.0588235294118:T=2,G=32,
+phiX174	1247	.	A	T	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:2:0.0714285714286:A=26,C=1,T=2,G=1,	0:0:0:A=35,
+phiX174	1250	.	A	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:2:0.0769230769231:A=24,C=2,	0:0:0:A=39,
+phiX174	1251	.	A	G	0	.	AC=3;AF=0.044776119403	GT:AC:AF:NC	0:0:0:A=29,	0:3:0.0789473684211:A=35,C=1,G=3,
+phiX174	1253	.	T	G	0	.	AC=4;AF=0.0526315789474	GT:AC:AF:NC	0:2:0.0645161290323:T=29,G=2,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1257	.	T	G	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:0:0:T=26,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1259	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:A=28,T=1,	0:2:0.0444444444444:A=43,C=2,G=1,
+phiX174	1274	.	G	C	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.0512820512821:C=2,G=37,	0:0:0:G=44,
+phiX174	1277	.	A	C	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=40,G=1,
+phiX174	1279	.	T	G	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0:0:T=39,G=1,	0:2:0.05:C=1,T=38,G=2,
+phiX174	1284	.	T	G,C	0	.	AC=3,2;AF=0.038961038961,0.025974025974	GT:AC:AF:NC	0:3,2:0.075,0.05:C=2,T=35,G=3,	0:0,0:0,0:A=1,T=37,
+phiX174	1289	.	A	C	0	.	AC=3;AF=0.0454545454545	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=28,G=1,
+phiX174	1296	.	A	C	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:0:0:A=38,	0:2:0.0769230769231:A=24,C=2,
+phiX174	1298	.	A	C	0	.	AC=3;AF=0.0491803278689	GT:AC:AF:NC	0:3,0:0.0810810810811,0:A=34,C=3,G=1,	0:0,0:0,0:A=24,C=1,T=1,G=1,
+phiX174	1306	.	T	G	0	.	AC=5;AF=0.0943396226415	GT:AC:AF:NC	0:5:0.178571428571:T=23,G=5,	0:0:0:C=1,T=25,
+phiX174	1307	.	T	G	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.107142857143:T=25,G=3,	0:0:0:C=1,T=27,
+phiX174	1314	.	A	G	0	.	AC=5;AF=0.0877192982456	GT:AC:AF:NC	0:2,0:0.0740740740741,0:A=25,C=1,G=2,	0:3,0:0.1,0:A=27,C=1,G=3,
+phiX174	1317	.	A	C	0	.	AC=2;AF=0.0322580645161	GT:AC:AF:NC	0:2:0.0740740740741:A=25,C=2,	0:0:0:A=35,
+phiX174	1320	.	C	G	0	.	AC=2;AF=0.0322580645161	GT:AC:AF:NC	0:2:0.0769230769231:C=24,G=2,	0:0:0:C=36,
+phiX174	1322	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.107142857143:C=3,G=25,	0:0:0:G=40,
+phiX174	1323	.	C	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:C=28,	0:2:0.0512820512821:C=37,T=1,G=2,
+phiX174	1329	.	T	C	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:2:0.0666666666667:C=2,T=28,	0:0:0:T=42,G=1,
+phiX174	1335	.	C	A	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.0625:A=2,C=30,	0:0:0:C=45,
+phiX174	1338	.	T	A	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:0:0:T=32,	0:2:0.047619047619:A=2,T=40,
+phiX174	1345	.	T	C	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0,0:0,0:T=36,G=1,	0:0,2:0,0.046511627907:C=2,T=41,G=1,
+phiX174	1347	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0512820512821:A=37,C=2,G=1,	0:0:0:A=43,
+phiX174	1348	.	T	G	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.05:T=38,G=2,	0:0:0:T=43,
+phiX174	1352	.	A	T	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:A=34,G=1,	0:2:0.0512820512821:A=37,T=2,
+phiX174	1354	.	T	G,A	0	.	AC=2,2;AF=0.027027027027,0.027027027027	GT:AC:AF:NC	0:2,0:0.0526315789474,0:T=36,G=2,	0:0,2:0,0.0555555555556:A=2,T=34,G=1,
+phiX174	1359	.	T	G	0	.	AC=3;AF=0.0405405405405	GT:AC:AF:NC	0:3:0.0714285714286:T=39,G=3,	0:0:0:T=32,G=1,
+phiX174	1366	.	G	C	0	.	AC=3;AF=0.0428571428571	GT:AC:AF:NC	0:3:0.0714285714286:C=3,T=1,G=39,	0:0:0:G=28,
+phiX174	1370	.	T	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:0:0:C=1,T=45,G=1,	0:2:0.0833333333333:A=1,T=22,G=2,
+phiX174	1373	.	T	G	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0408163265306:T=47,G=2,	0:0:0:T=25,
+phiX174	1380	.	G	C	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:0,2:0,0.0384615384615:C=2,T=1,G=50,	0:0,0:0,0:T=1,G=32,
+phiX174	1381	.	T	G	0	.	AC=3;AF=0.0361445783133	GT:AC:AF:NC	0:3:0.0576923076923:A=1,T=49,G=3,	0:0:0:T=31,
+phiX174	1384	.	T	G	0	.	AC=4;AF=0.0512820512821	GT:AC:AF:NC	0:4:0.0816326530612:T=45,G=4,	0:0:0:T=29,G=1,
+phiX174	1385	.	T	G	0	.	AC=4;AF=0.0512820512821	GT:AC:AF:NC	0:4:0.0816326530612:T=45,G=4,	0:0:0:C=1,T=29,
+phiX174	1391	.	A	C	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:2:0.0408163265306:A=47,C=2,	0:0:0:A=30,C=1,
+phiX174	1393	.	C	A	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:2:0.0425531914894:A=2,C=45,	0:0:0:C=32,
+phiX174	1396	.	T	G	0	.	AC=3;AF=0.04	GT:AC:AF:NC	0:3:0.0697674418605:T=40,G=3,	0:0:0:T=32,
+phiX174	1397	.	A	C	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:0:0:A=42,T=1,	0:2:0.0625:A=30,C=2,
+phiX174	1400	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.047619047619:A=40,C=2,G=1,	0:0:0:A=31,
+phiX174	1401	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.047619047619:A=40,C=2,	0:0:0:A=31,
+phiX174	1434	.	C	T	0	.	AC=2;AF=0.0571428571429	GT:AC:AF:NC	0:2:0.142857142857:C=12,T=2,	0:0:0:C=21,
+phiX174	1438	.	G	C	0	.	AC=2;AF=0.0526315789474	GT:AC:AF:NC	0:0:0:G=15,	0:2:0.0869565217391:C=2,G=21,
+phiX174	1450	.	T	G	0	.	AC=3;AF=0.0789473684211	GT:AC:AF:NC	0:3:0.176470588235:A=1,C=1,T=14,G=3,	0:0:0:T=21,G=1,
+phiX174	1459	.	T	G	0	.	AC=3;AF=0.075	GT:AC:AF:NC	0:3:0.142857142857:C=1,T=18,G=3,	0:0:0:T=19,
+phiX174	1470	.	C	A	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:0:0:C=29,	0:2:0.0869565217391:A=2,C=21,
+phiX174	1484	.	C	G	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:2:0.0740740740741:A=1,C=25,G=2,	0:0:0:C=25,
+phiX174	1487	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0689655172414:T=27,G=2,	0:0:0:T=26,
+phiX174	1491	.	G	T	0	.	AC=2;AF=0.037037037037	GT:AC:AF:NC	0:0:0:T=1,G=26,	0:2:0.0714285714286:T=2,G=26,
+phiX174	1503	.	A	C	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:0:0:A=20,C=1,	0:2:0.0833333333333:A=22,C=2,
+phiX174	1536	.	A	C	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.111111111111:A=24,C=3,	0:0:0:A=28,C=1,
+phiX174	1541	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0740740740741:T=25,G=2,	0:0:0:T=28,
+phiX174	1553	.	A	G	0	.	AC=2;AF=0.0408163265306	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=1,G=2,	0:0:0:A=28,
+phiX174	1566	.	C	G	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0:0:C=30,T=1,	0:2:0.0571428571429:C=33,G=2,
+phiX174	1572	.	T	G	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:2:0.0625:T=30,G=2,	0:0:0:T=32,
+phiX174	1574	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0882352941176:C=3,G=31,	0:0:0:G=34,
+phiX174	1575	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=33,T=1,
+phiX174	1577	.	A	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:0:0:A=35,	0:3:0.0909090909091:A=30,C=3,
+phiX174	1581	.	T	G	0	.	AC=2;AF=0.0285714285714	GT:AC:AF:NC	0:2:0.0555555555556:C=1,T=34,G=2,	0:0:0:T=34,G=1,
+phiX174	1591	.	A	C	0	.	AC=3;AF=0.037037037037	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=43,C=1,
+phiX174	1597	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0606060606061:T=31,G=2,	0:0:0:C=1,T=45,
+phiX174	1603	.	T	G	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0625:T=30,G=2,	0:0:0:T=50,
+phiX174	1606	.	T	G	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0666666666667:A=1,C=1,T=28,G=2,	0:0:0:T=52,
+phiX174	1611	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0769230769231:A=24,C=2,	0:0:0:A=56,C=1,
+phiX174	1613	.	A	G	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:0:0:A=25,C=1,G=1,	0:2:0.0344827586207:A=56,G=2,
+phiX174	1618	.	C	A	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0666666666667:A=2,C=28,G=1,	0:0:0:A=1,C=57,
+phiX174	1619	.	C	A	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0666666666667:A=2,C=28,	0:0:0:C=57,
+phiX174	1621	.	A	C	0	.	AC=3;AF=0.0352941176471	GT:AC:AF:NC	0:0:0:A=31,T=1,G=1,	0:3:0.0555555555556:A=51,C=3,
+phiX174	1622	.	G	T	0	.	AC=2;AF=0.0224719101124	GT:AC:AF:NC	0:0:0:A=1,G=35,	0:2:0.037037037037:T=2,G=52,
+phiX174	1624	.	A	G	0	.	AC=2;AF=0.0235294117647	GT:AC:AF:NC	0:2:0.0555555555556:A=34,T=1,G=2,	0:0:0:A=49,
+phiX174	1627	.	T	G,C	0	.	AC=2,2;AF=0.0246913580247,0.0246913580247	GT:AC:AF:NC	0:0,0:0,0:T=37,G=1,	0:2,2:0.0454545454545,0.0454545454545:C=2,T=40,G=2,
+phiX174	1630	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0526315789474:C=1,T=36,G=2,	0:0:0:T=40,
+phiX174	1632	.	A	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=41,
+phiX174	1634	.	T	G	0	.	AC=4;AF=0.0493827160494	GT:AC:AF:NC	0:2:0.047619047619:C=1,T=40,G=2,	0:2:0.0512820512821:T=37,G=2,
+phiX174	1635	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0714285714286:T=39,G=3,	0:0:0:T=40,
+phiX174	1639	.	G	A	0	.	AC=3;AF=0.0405405405405	GT:AC:AF:NC	0:3:0.075:A=3,T=1,G=37,	0:0:0:G=34,
+phiX174	1645	.	T	G	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0425531914894:T=45,G=2,	0:0:0:T=27,G=1,
+phiX174	1647	.	A	T,G,C	0	.	AC=2,2,2;AF=0.0253164556962,0.0253164556962,0.0253164556962	GT:AC:AF:NC	0:0,2,2:0,0.0384615384615,0.0384615384615:A=48,C=2,G=2,	0:2,0,0:0.0740740740741,0,0:A=25,T=2,
+phiX174	1652	.	G	A	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.037037037037:A=2,G=52,	0:0:0:G=29,
+phiX174	1656	.	T	G	0	.	AC=3;AF=0.0348837209302	GT:AC:AF:NC	0:3:0.0555555555556:T=51,G=3,	0:0:0:T=32,G=1,
+phiX174	1658	.	A	C	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.04:A=48,C=2,	0:0:0:A=33,
+phiX174	1659	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0625:C=1,T=45,G=3,	0:0:0:T=34,
+phiX174	1663	.	T	G	0	.	AC=4;AF=0.0454545454545	GT:AC:AF:NC	0:0:0:A=1,T=53,	0:4:0.114285714286:T=31,G=4,
+phiX174	1665	.	C	A	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0384615384615:A=2,C=50,G=1,	0:0:0:C=42,
+phiX174	1670	.	G	C	0	.	AC=2;AF=0.0224719101124	GT:AC:AF:NC	0:0,2:0,0.0408163265306:A=1,C=2,T=1,G=47,	0:0,0:0,0:T=1,G=40,
+phiX174	1671	.	G	T	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0384615384615:C=1,T=2,G=50,	0:0:0:G=42,
+phiX174	1672	.	A	G	0	.	AC=3;AF=0.0315789473684	GT:AC:AF:NC	0:3:0.0545454545455:A=52,G=3,	0:0:0:A=40,T=1,
+phiX174	1673	.	G	T	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0377358490566:C=1,T=2,G=51,	0:0:0:G=41,
+phiX174	1674	.	G	T	0	.	AC=4;AF=0.04	GT:AC:AF:NC	0:4:0.0714285714286:T=4,G=52,	0:0:0:G=44,
+phiX174	1677	.	A	C	0	.	AC=3;AF=0.031914893617	GT:AC:AF:NC	0:3:0.0566037735849:A=50,C=3,N=1,	0:0:0:A=41,
+phiX174	1678	.	A	C	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:0:0:A=54,C=1,G=1,	0:2:0.05:A=38,C=2,
+phiX174	1682	.	T	C	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0408163265306:C=2,T=47,G=1,	0:0:0:C=1,T=38,
+phiX174	1685	.	T	G	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:2:0.046511627907:A=1,T=41,G=2,	0:0:0:T=41,G=1,
+phiX174	1686	.	A	C	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:0:0:A=39,C=1,T=1,G=1,	0:2:0.0512820512821:A=37,C=2,
+phiX174	1687	.	T	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.047619047619:C=2,T=40,	0:0:0:A=1,T=40,
+phiX174	1688	.	G	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.05:C=2,T=1,G=38,	0:0:0:G=41,
+phiX174	1689	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0487804878049:A=39,C=2,T=1,G=1,	0:0:0:A=41,C=1,
+phiX174	1691	.	G	C	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.05:C=2,T=1,G=38,	0:0:0:G=37,
+phiX174	1693	.	T	G	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:0:0:T=42,G=1,	0:2:0.047619047619:T=40,G=2,
+phiX174	1695	.	A	C	0	.	AC=2;AF=0.0232558139535	GT:AC:AF:NC	0:2:0.046511627907:A=41,C=2,	0:0:0:A=43,C=1,
+phiX174	1702	.	T	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.0512820512821:A=1,C=2,T=37,	0:0:0:C=1,T=42,
+phiX174	1707	.	T	C	0	.	AC=2;AF=0.0232558139535	GT:AC:AF:NC	0:2:0.05:C=2,T=38,	0:0:0:T=46,G=1,
+phiX174	1708	.	A	C	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:2:0.0526315789474:A=36,C=2,	0:0:0:A=46,C=1,G=1,
+phiX174	1710	.	T	C	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:0:0:C=1,T=34,	0:2:0.0434782608696:C=2,T=44,
+phiX174	1723	.	T	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0588235294118:C=2,T=32,	0:0:0:T=48,
+phiX174	1724	.	A	C	0	.	AC=4;AF=0.0481927710843	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:2:0.0416666666667:A=46,C=2,
+phiX174	1725	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0606060606061:A=31,C=2,	0:0:0:A=49,
+phiX174	1726	.	T	G	0	.	AC=3;AF=0.037037037037	GT:AC:AF:NC	0:3:0.0882352941176:T=31,G=3,	0:0:0:C=1,T=47,
+phiX174	1730	.	T	G	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:C=1,T=28,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1739	.	G	T	0	.	AC=3;AF=0.0434782608696	GT:AC:AF:NC	0:3:0.09375:T=3,G=29,	0:0:0:G=37,
+phiX174	1746	.	A	C	0	.	AC=2;AF=0.0338983050847	GT:AC:AF:NC	0:2:0.0689655172414:A=27,C=2,	0:0:0:A=30,C=1,G=1,
+phiX174	1749	.	T	G,C	0	.	AC=3,2;AF=0.044776119403,0.0298507462687	GT:AC:AF:NC	0:3,2:0.0857142857143,0.0571428571429:C=2,T=30,G=3,	0:0,0:0,0:T=32,G=1,
+phiX174	1755	.	G	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.05:C=2,G=38,	0:0:0:C=1,G=33,
+phiX174	1756	.	A	G,C	0	.	AC=2,2;AF=0.0281690140845,0.0281690140845	GT:AC:AF:NC	0:0,2:0,0.0526315789474:A=36,C=2,T=1,G=1,	0:2,0:0.0606060606061,0:A=31,C=1,G=2,
+phiX174	1761	.	A	G	0	.	AC=2;AF=0.0285714285714	GT:AC:AF:NC	0:2:0.0512820512821:A=37,C=1,G=2,	0:0:0:A=31,
+phiX174	1764	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=28,
+phiX174	1766	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0487804878049:A=39,C=2,	0:0:0:A=27,
+phiX174	1769	.	T	G	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:2:0.0526315789474:A=1,C=1,T=36,G=2,	0:0:0:T=26,G=1,
+phiX174	1771	.	G	C	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:2:0.0526315789474:C=2,G=36,	0:0:0:G=29,
+phiX174	1772	.	T	G	0	.	AC=4;AF=0.0597014925373	GT:AC:AF:NC	0:4:0.108108108108:C=1,T=33,G=4,	0:0:0:T=30,
+phiX174	1774	.	A	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0810810810811:A=34,G=3,	0:0:0:A=31,
+phiX174	1775	.	G	A	0	.	AC=2;AF=0.030303030303	GT:AC:AF:NC	0:2:0.0571428571429:A=2,G=33,	0:0:0:G=31,
+phiX174	1776	.	G	T	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0540540540541:T=2,G=35,	0:0:0:G=32,
+phiX174	1778	.	C	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0526315789474:C=36,G=2,	0:0:0:C=31,
+phiX174	1779	.	A	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.075:A=37,C=3,	0:0:0:A=28,
+phiX174	1781	.	T	G	0	.	AC=5;AF=0.0714285714286	GT:AC:AF:NC	0:2:0.0487804878049:C=1,T=39,G=2,	0:3:0.103448275862:T=26,G=3,
+phiX174	1782	.	T	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.075:T=37,G=3,	0:0:0:A=1,T=28,
+phiX174	1783	.	T	C	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:2:0.0512820512821:C=2,T=37,	0:0:0:T=28,G=1,
+phiX174	1788	.	G	T	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:0:0:A=1,T=1,G=29,	0:2:0.0689655172414:T=2,G=27,
+phiX174	1792	.	T	G	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:2:0.0606060606061:T=31,G=2,	0:0:0:T=25,G=1,
+phiX174	1802	.	A	C	0	.	AC=2;AF=0.030303030303	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=31,C=1,
+phiX174	1804	.	C	T	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0588235294118:A=1,C=32,T=2,	0:0:0:C=34,
+phiX174	1805	.	T	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0857142857143:T=32,G=3,	0:0:0:T=33,G=1,
+phiX174	1806	.	A	C	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=34,G=1,
+phiX174	1824	.	G	C	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0714285714286:C=2,T=1,G=26,	0:0:0:G=43,
+phiX174	1825	.	T	G	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0689655172414:T=27,G=2,	0:0:0:T=42,G=1,
+phiX174	1833	.	T	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:0:0:A=1,T=24,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1843	.	T	G	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:2:0.08:A=1,T=23,G=2,	0:0:0:T=38,
+phiX174	1847	.	A	C	0	.	AC=2;AF=0.0333333333333	GT:AC:AF:NC	0:2:0.0833333333333:A=22,C=2,T=1,G=1,	0:0:0:A=36,
+phiX174	1854	.	T	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:T=26,	0:2:0.0487804878049:T=39,G=2,
+phiX174	1860	.	T	G	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:0:0:A=1,T=20,	0:2:0.0606060606061:T=31,G=2,
+phiX174	1863	.	C	G	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=17,G=2,	0:0:0:C=33,
+phiX174	1866	.	T	G	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:0:0:T=19,G=1,	0:2:0.0645161290323:T=29,G=2,
+phiX174	1875	.	T	A	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:2:0.0952380952381:A=2,T=19,	0:0:0:T=32,G=1,
+phiX174	1876	.	T	G	0	.	AC=3;AF=0.0588235294118	GT:AC:AF:NC	0:3:0.157894736842:C=1,T=16,G=3,	0:0:0:T=32,G=1,
+phiX174	1882	.	T	G	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:2:0.105263157895:T=17,G=2,	0:0:0:T=34,
+phiX174	1889	.	A	C	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:2:0.0869565217391:A=21,C=2,	0:0:0:A=27,
+phiX174	1913	.	G	C	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:0:0:G=27,	0:2:0.0869565217391:C=2,G=21,
+phiX174	1915	.	T	G	0	.	AC=2;AF=0.0425531914894	GT:AC:AF:NC	0:0:0:A=1,T=24,	0:2:0.0869565217391:T=21,G=2,
+phiX174	1917	.	A	T	0	.	AC=2;AF=0.0444444444444	GT:AC:AF:NC	0:2,0:0.0833333333333,0:A=22,T=2,G=1,	0:0,0:0,0:A=21,G=1,
+phiX174	1930	.	T	A	0	.	AC=2;AF=0.0526315789474	GT:AC:AF:NC	0:2:0.0909090909091:A=2,T=20,	0:0:0:T=16,
+phiX174	1934	.	A	C	0	.	AC=2;AF=0.0512820512821	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=2,T=1,	0:0:0:A=18,C=1,
+phiX174	1940	.	A	C	0	.	AC=2;AF=0.046511627907	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=2,	0:0:0:A=22,C=1,
+phiX174	1942	.	T	G	0	.	AC=2;AF=0.047619047619	GT:AC:AF:NC	0:0:0:T=19,	0:2:0.0869565217391:T=21,G=2,
+phiX174	1945	.	T	G	0	.	AC=3;AF=0.0769230769231	GT:AC:AF:NC	0:0:0:T=15,G=1,	0:3:0.125:T=21,G=3,
+phiX174	1954	.	T	C	0	.	AC=2;AF=0.0625	GT:AC:AF:NC	0:0,0:0,0:T=12,G=1,	0:0,2:0,0.1:C=2,T=18,G=1,
+phiX174	1959	.	T	G	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:2:0.153846153846:T=11,G=2,	0:0:0:T=23,
+phiX174	1994	.	A	C	0	.	AC=2;AF=0.0512820512821	GT:AC:AF:NC	0:2:0.153846153846:A=11,C=2,	0:0:0:A=26,
+phiX174	1998	.	A	G	0	.	AC=2;AF=0.05	GT:AC:AF:NC	0:2:0.142857142857:A=12,G=2,	0:0:0:A=26,
diff --git a/tool_collections/vcflib/vcfprimers/test-data/vcflib-test-genome-phix.fa b/tool_collections/vcflib/vcfprimers/test-data/vcflib-test-genome-phix.fa
new file mode 100644
index 00000000000..48a6b46678a
--- /dev/null
+++ b/tool_collections/vcflib/vcfprimers/test-data/vcflib-test-genome-phix.fa
@@ -0,0 +1,2 @@
+>phiX174
+GAGTTTTATCGCTTCCATGACGCAGAAGTTAACACTTTCGGATATTTCTGATGAGTCGAAAAATTATCTTGATAAAGCAGGAATTACTACTGCTTGTTTACGAATTAAATCGAAGTGGACTGCTGGCGGAAAATGAGAAAATTCGACCTATCCTTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGTTTAGATATGAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTGGATTACTATCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAGAAATCATGAGTCAAGTTACTGAACAATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTAACCGAAGATGATTTCGATTTTCTGACGAGTAACAAAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCTTGCGTTTATGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTTTATTGCTGCCGTCATTGCTTATTATGTTCATCCCGTCAACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTACGGAAAACATTATTAATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAACACTGACGTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAAATTACGTGCaGAAGGAGTGATGTAATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATTTCAACTACTCCGGTTATCGCTGGCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTTTTTATGTCCCTCATCGTCACGTTTATGGTGAACAGTGGATTAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCCGACTGTTAACACTACTGGTTATATTGACCATGCCGCTTTTCTTGGCACGATTAACCCTGATACCAATAAAATCCCTAAGCATTTGTTTCAGGGTTATTTGAATATCTATAACAACTATTTTAAAGCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATCAAGATGATGCTCGTTATGGTTTCCGTTGCTGCCATCTCAAAAACATTTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGACTTCTACCACATCTATTGACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGCATACTGACCAAGAACGTGATTACTTCATGCAGCGTTACCgTGATGTTATTTCTTCATTTGGAGGTAAAACCTCTTATGACGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCTATGATGTTGATGGAACTGACCAAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAACATTCTGTGCCGCGTTTCTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGCGACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGCTTTGACTTATACCGATATTGCTGGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTGCTGAGGGTCAGTGGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGATTATGACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGAATAGTCAGGTTAAATTTAATGTGACCGTTTATCGCAATCTGCCGACCACTCGCGATTCAATCATGACTTCGTGATAAAAGATTGAGTGTGAGGTTATAACGCCGAAGCGGTAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACCAAGCGAAGCGCGGTAGGTTTTCTGCTTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTCGCCATAATTCAAACTTTTTTTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTTTACAGACACCTAAAGCTACATCGTCAACGTTATATTTTGATAGTTTGACGGTTAATGCTGGTAATGGTGGTTTTCTTCATTGCATTCAGATGGATACATCTGTCAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGCCGACCCTAAATTTTTTGCCTGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTCCCGACTGCCTATGATGTTTATCCTTTGAATGGTCGCCATGATGGTGGTTATTATACCGTCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTACGCCGGGCAATAAtGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGGTGATTTATGTTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAAAGCCGCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACCGATAACAATACTGTAGGCATGGGTGATGCTGGTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCAACTGGACAATCAGAAAGAGATTGCCGAGATGCAAAATGAGACTCAAAAAGAGATTGCTGGCATTCAGTCGGCGACTTCACGCCAGAATACGAAAGACCAGGTATATGCACAAAATGAGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGCGTTGCGTCTATTATGGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAAGCTCAAACGGCTGGTCAGTATTTTACCAATGACCAAATCAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCTCTTCTCATATTGGCGCTACTGCAAAGGATATTTCTAATGTCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTTTTCATGGTATTGATAAAGCTGTTGCCGATACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTCTAATTTGTCTAGGAAATAACCGTCAGGATTGACACCCTCCCAATTGTATGTTTTCATGCCTCCAAATCTTGGAGGCTTTTTTATGGTTCGTTCTTATTACCCTTCTGAATGTCACGCTGATTATTTTGACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAATGCTTGGCTTCCATAAGCAGATGGATAACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAGGGCGTTGAGTTCGATAATGGTGATATGTATGTTGACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTACTGAGAAGTTAATGGATGAATTGGCACAATGCTACAATGTGCTCCCCCAACTTGATATTAATAACACTATAGACCACCGCCCCGAAGGGGACGAAAAATGGTTTTTAGAGAACGAGAAGACGGTTACGCAGTTTTGCCGCAAGCTGGCTGCTGAACGCCCTCTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTaCTATTCAGCGTTTGATGAATGCAATGCGACAGGCTCATGCTGATGGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCTGACGACCGATTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGTGACTATTTTCGTGATATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATGATTCACACGCCGACTGCTATCAGTATTTTTGTGTGCCTGAGTATGGTACAGCTAATGGCCGTCTTCATTTCCATGCGGTGCAtTTTATGCGGACACTTCCTACAGGTAGCGTTGACCCTAATTTTGGTCGTCGGGTACGCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGTGGCCTTATGGTTACAGTATGCCCATCGCAGTTCGCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTAAATACGTTAACAAAAAGTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCACTAAAAACCAAGCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAGAATGAGCCGCAACTTCGGGATGAAAATGCTCACAATGACAAATCTGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGATATTGAAGCAGAACGCAAAAAGAGAGATGAGATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTTTGGCGGCGCAACCTGTGACGACAAATCTGCTCAAATTTATGCGCGCTTCGATAAAAATGATTGGCGTATCCAACCTGCA
diff --git a/tool_collections/vcflib/vcfprimers/test-data/vcfprimers-test1.fasta b/tool_collections/vcflib/vcfprimers/test-data/vcfprimers-test1.fasta
new file mode 100644
index 00000000000..6f5b12772b9
--- /dev/null
+++ b/tool_collections/vcflib/vcfprimers/test-data/vcfprimers-test1.fasta
@@ -0,0 +1,828 @@
+>phiX174_1015_LEFT
+ATTCA
+>phiX174_1015_RIGHT
+ACTGG
+>phiX174_1016_LEFT
+TTCAA
+>phiX174_1016_RIGHT
+CTGGC
+>phiX174_1038_LEFT
+GCCGC
+>phiX174_1038_RIGHT
+TGACC
+>phiX174_1045_LEFT
+GACCT
+>phiX174_1045_RIGHT
+TCCCA
+>phiX174_1048_LEFT
+CTTTC
+>phiX174_1048_RIGHT
+CATCT
+>phiX174_1050_LEFT
+TTCCC
+>phiX174_1050_RIGHT
+TCTTG
+>phiX174_1051_LEFT
+TCCCA
+>phiX174_1051_RIGHT
+CTTGG
+>phiX174_1054_LEFT
+CATCT
+>phiX174_1054_RIGHT
+GGCTT
+>phiX174_1076_LEFT
+AGATT
+>phiX174_1076_RIGHT
+GTCGT
+>phiX174_1080_LEFT
+TGGTC
+>phiX174_1080_RIGHT
+TCTTA
+>phiX174_1092_LEFT
+TACCA
+>phiX174_1092_RIGHT
+TTCAA
+>phiX174_1093_LEFT
+ACCAT
+>phiX174_1093_RIGHT
+TCAAC
+>phiX174_1102_LEFT
+ACTAC
+>phiX174_1102_RIGHT
+CCGGT
+>phiX174_1107_LEFT
+TCCGG
+>phiX174_1107_RIGHT
+TATCG
+>phiX174_1110_LEFT
+GGTTA
+>phiX174_1110_RIGHT
+CGCTG
+>phiX174_1121_LEFT
+GCGAC
+>phiX174_1121_RIGHT
+CCTTC
+>phiX174_1124_LEFT
+ACTCC
+>phiX174_1124_RIGHT
+TCGAG
+>phiX174_1125_LEFT
+CTCCT
+>phiX174_1125_RIGHT
+CGAGA
+>phiX174_1131_LEFT
+CGAGA
+>phiX174_1131_RIGHT
+GGACG
+>phiX174_1141_LEFT
+GCCGT
+>phiX174_1141_RIGHT
+GGCGC
+>phiX174_1145_LEFT
+TTGGC
+>phiX174_1145_RIGHT
+CTCTC
+>phiX174_1147_LEFT
+GGCGC
+>phiX174_1147_RIGHT
+CTCCG
+>phiX174_1149_LEFT
+CGCTC
+>phiX174_1149_RIGHT
+CCGTC
+>phiX174_1156_LEFT
+CGTCT
+>phiX174_1156_RIGHT
+TCTCC
+>phiX174_1159_LEFT
+CTTTC
+>phiX174_1159_RIGHT
+CCATT
+>phiX174_1164_LEFT
+TCCAT
+>phiX174_1164_RIGHT
+GCGTC
+>phiX174_1168_LEFT
+TTGCG
+>phiX174_1168_RIGHT
+CGTGG
+>phiX174_1171_LEFT
+CGTCG
+>phiX174_1171_RIGHT
+GGCCT
+>phiX174_1174_LEFT
+CGTGG
+>phiX174_1174_RIGHT
+CTTGC
+>phiX174_1178_LEFT
+GCCTT
+>phiX174_1178_RIGHT
+CTATT
+>phiX174_1187_LEFT
+TTGAC
+>phiX174_1187_RIGHT
+CTACT
+>phiX174_1189_LEFT
+GACTC
+>phiX174_1189_RIGHT
+ACTGT
+>phiX174_1200_LEFT
+AGACA
+>phiX174_1200_RIGHT
+TTTTA
+>phiX174_1202_LEFT
+ACATT
+>phiX174_1202_RIGHT
+TTACT
+>phiX174_1205_LEFT
+TTTTT
+>phiX174_1205_RIGHT
+CTTTT
+>phiX174_1207_LEFT
+TTTAC
+>phiX174_1207_RIGHT
+TTTTA
+>phiX174_1214_LEFT
+TTTAT
+>phiX174_1214_RIGHT
+TCCCT
+>phiX174_1216_LEFT
+TATGT
+>phiX174_1216_RIGHT
+CCTCA
+>phiX174_1217_LEFT
+ATGTC
+>phiX174_1217_RIGHT
+CTCAT
+>phiX174_1219_LEFT
+GTCCC
+>phiX174_1219_RIGHT
+CATCG
+>phiX174_1223_LEFT
+CTCAT
+>phiX174_1223_RIGHT
+GTCAC
+>phiX174_1227_LEFT
+TCGTC
+>phiX174_1227_RIGHT
+CGTTT
+>phiX174_1232_LEFT
+ACGTT
+>phiX174_1232_RIGHT
+ATGGT
+>phiX174_1236_LEFT
+TTATG
+>phiX174_1236_RIGHT
+TGAAC
+>phiX174_1237_LEFT
+TATGG
+>phiX174_1237_RIGHT
+GAACA
+>phiX174_1240_LEFT
+GGTGA
+>phiX174_1240_RIGHT
+CAGTG
+>phiX174_1245_LEFT
+ACAGT
+>phiX174_1245_RIGHT
+GATTA
+>phiX174_1247_LEFT
+AGTGG
+>phiX174_1247_RIGHT
+TTAAG
+>phiX174_1250_LEFT
+GGATT
+>phiX174_1250_RIGHT
+AGTTC
+>phiX174_1251_LEFT
+GATTA
+>phiX174_1251_RIGHT
+GTTCA
+>phiX174_1253_LEFT
+TTAAG
+>phiX174_1253_RIGHT
+TCATG
+>phiX174_1257_LEFT
+GTTCA
+>phiX174_1257_RIGHT
+GAAGG
+>phiX174_1259_LEFT
+TCATG
+>phiX174_1259_RIGHT
+AGGAT
+>phiX174_1274_LEFT
+TTAAT
+>phiX174_1274_RIGHT
+CCACT
+>phiX174_1277_LEFT
+ATGCC
+>phiX174_1277_RIGHT
+CTCCT
+>phiX174_1279_LEFT
+GCCAC
+>phiX174_1279_RIGHT
+CCTCT
+>phiX174_1284_LEFT
+TCCTC
+>phiX174_1284_RIGHT
+CCCGA
+>phiX174_1289_LEFT
+TCCCG
+>phiX174_1289_RIGHT
+CTGTT
+>phiX174_1296_LEFT
+TGTTA
+>phiX174_1296_RIGHT
+CACTA
+>phiX174_1298_LEFT
+TTAAC
+>phiX174_1298_RIGHT
+CTACT
+>phiX174_1306_LEFT
+ACTGG
+>phiX174_1306_RIGHT
+TATAT
+>phiX174_1307_LEFT
+CTGGT
+>phiX174_1307_RIGHT
+ATATT
+>phiX174_1314_LEFT
+TATTG
+>phiX174_1314_RIGHT
+CCATG
+>phiX174_1317_LEFT
+TGACC
+>phiX174_1317_RIGHT
+TGCCG
+>phiX174_1320_LEFT
+CCATG
+>phiX174_1320_RIGHT
+CGCTT
+>phiX174_1322_LEFT
+ATGCC
+>phiX174_1322_RIGHT
+CTTTT
+>phiX174_1323_LEFT
+TGCCG
+>phiX174_1323_RIGHT
+TTTTC
+>phiX174_1329_LEFT
+TTTTC
+>phiX174_1329_RIGHT
+TGGCA
+>phiX174_1335_LEFT
+TGGCA
+>phiX174_1335_RIGHT
+GATTA
+>phiX174_1338_LEFT
+CACGA
+>phiX174_1338_RIGHT
+TAACC
+>phiX174_1345_LEFT
+AACCC
+>phiX174_1345_RIGHT
+GATAC
+>phiX174_1347_LEFT
+CCCTG
+>phiX174_1347_RIGHT
+TACCA
+>phiX174_1348_LEFT
+CCTGA
+>phiX174_1348_RIGHT
+ACCAA
+>phiX174_1352_LEFT
+ATACC
+>phiX174_1352_RIGHT
+ATAAA
+>phiX174_1354_LEFT
+ACCAA
+>phiX174_1354_RIGHT
+AAAAT
+>phiX174_1359_LEFT
+TAAAA
+>phiX174_1359_RIGHT
+CCCTA
+>phiX174_1366_LEFT
+CCTAA
+>phiX174_1366_RIGHT
+CATTT
+>phiX174_1370_LEFT
+AGCAT
+>phiX174_1370_RIGHT
+TGTTT
+>phiX174_1373_LEFT
+ATTTG
+>phiX174_1373_RIGHT
+TTCAG
+>phiX174_1380_LEFT
+TCAGG
+>phiX174_1380_RIGHT
+TTATT
+>phiX174_1381_LEFT
+CAGGG
+>phiX174_1381_RIGHT
+TATTT
+>phiX174_1384_LEFT
+GGTTA
+>phiX174_1384_RIGHT
+TTGAA
+>phiX174_1385_LEFT
+GTTAT
+>phiX174_1385_RIGHT
+TGAAT
+>phiX174_1391_LEFT
+TGAAT
+>phiX174_1391_RIGHT
+TCTAT
+>phiX174_1393_LEFT
+AATAT
+>phiX174_1393_RIGHT
+TATAA
+>phiX174_1396_LEFT
+ATCTA
+>phiX174_1396_RIGHT
+AACAA
+>phiX174_1397_LEFT
+TCTAT
+>phiX174_1397_RIGHT
+ACAAC
+>phiX174_1400_LEFT
+ATAAC
+>phiX174_1400_RIGHT
+ACTAT
+>phiX174_1401_LEFT
+TAACA
+>phiX174_1401_RIGHT
+CTATT
+>phiX174_1434_LEFT
+CCGTA
+>phiX174_1434_RIGHT
+CGAGG
+>phiX174_1438_LEFT
+ACCGA
+>phiX174_1438_RIGHT
+GCTAA
+>phiX174_1450_LEFT
+CCTAA
+>phiX174_1450_RIGHT
+GAGCT
+>phiX174_1459_LEFT
+CTTAA
+>phiX174_1459_RIGHT
+CAAGA
+>phiX174_1470_LEFT
+TGATG
+>phiX174_1470_RIGHT
+TCGTT
+>phiX174_1484_LEFT
+GTTTC
+>phiX174_1484_RIGHT
+GTTGC
+>phiX174_1487_LEFT
+TCCGT
+>phiX174_1487_RIGHT
+GCTGC
+>phiX174_1491_LEFT
+TTGCT
+>phiX174_1491_RIGHT
+CCATC
+>phiX174_1503_LEFT
+CAAAA
+>phiX174_1503_RIGHT
+CATTT
+>phiX174_1536_LEFT
+GACTG
+>phiX174_1536_RIGHT
+GCTTT
+>phiX174_1541_LEFT
+AGCTT
+>phiX174_1541_RIGHT
+CTCGC
+>phiX174_1553_LEFT
+AAATG
+>phiX174_1553_RIGHT
+CGACT
+>phiX174_1566_LEFT
+TACCA
+>phiX174_1566_RIGHT
+ATCTA
+>phiX174_1572_LEFT
+ATCTA
+>phiX174_1572_RIGHT
+TGACA
+>phiX174_1574_LEFT
+CTATT
+>phiX174_1574_RIGHT
+ACATT
+>phiX174_1575_LEFT
+TATTG
+>phiX174_1575_RIGHT
+CATTA
+>phiX174_1577_LEFT
+TTGAC
+>phiX174_1577_RIGHT
+TTATG
+>phiX174_1581_LEFT
+CATTA
+>phiX174_1581_RIGHT
+GGGTC
+>phiX174_1591_LEFT
+CTGCA
+>phiX174_1591_RIGHT
+GCTGC
+>phiX174_1597_LEFT
+GCTGC
+>phiX174_1597_RIGHT
+TATGC
+>phiX174_1603_LEFT
+TATGC
+>phiX174_1603_RIGHT
+AATTT
+>phiX174_1606_LEFT
+GCTAA
+>phiX174_1606_RIGHT
+TTGCA
+>phiX174_1611_LEFT
+TTTGC
+>phiX174_1611_RIGHT
+TACTG
+>phiX174_1613_LEFT
+TGCAT
+>phiX174_1613_RIGHT
+CTGAC
+>phiX174_1618_LEFT
+ACTGA
+>phiX174_1618_RIGHT
+CAAGA
+>phiX174_1619_LEFT
+CTGAC
+>phiX174_1619_RIGHT
+AAGAA
+>phiX174_1621_LEFT
+GACCA
+>phiX174_1621_RIGHT
+GAACG
+>phiX174_1622_LEFT
+ACCAA
+>phiX174_1622_RIGHT
+AACGT
+>phiX174_1624_LEFT
+CAAGA
+>phiX174_1624_RIGHT
+CGTGA
+>phiX174_1627_LEFT
+GAACG
+>phiX174_1627_RIGHT
+GATTA
+>phiX174_1630_LEFT
+CGTGA
+>phiX174_1630_RIGHT
+TACTT
+>phiX174_1632_LEFT
+TGATT
+>phiX174_1632_RIGHT
+CTTCA
+>phiX174_1634_LEFT
+ATTAC
+>phiX174_1634_RIGHT
+TCATG
+>phiX174_1635_LEFT
+TTACT
+>phiX174_1635_RIGHT
+CATGC
+>phiX174_1639_LEFT
+TTCAT
+>phiX174_1639_RIGHT
+CAGCG
+>phiX174_1645_LEFT
+CAGCG
+>phiX174_1645_RIGHT
+TACCg
+>phiX174_1647_LEFT
+GCGTT
+>phiX174_1647_RIGHT
+CCgTG
+>phiX174_1652_LEFT
+ACCgT
+>phiX174_1652_RIGHT
+ATGTT
+>phiX174_1656_LEFT
+TGATG
+>phiX174_1656_RIGHT
+TATTT
+>phiX174_1658_LEFT
+ATGTT
+>phiX174_1658_RIGHT
+TTTCT
+>phiX174_1659_LEFT
+TGTTA
+>phiX174_1659_RIGHT
+TTCTT
+>phiX174_1663_LEFT
+ATTTC
+>phiX174_1663_RIGHT
+TCATT
+>phiX174_1665_LEFT
+TTCTT
+>phiX174_1665_RIGHT
+ATTTG
+>phiX174_1670_LEFT
+CATTT
+>phiX174_1670_RIGHT
+GAGGT
+>phiX174_1671_LEFT
+ATTTG
+>phiX174_1671_RIGHT
+AGGTA
+>phiX174_1672_LEFT
+TTTGG
+>phiX174_1672_RIGHT
+GGTAA
+>phiX174_1673_LEFT
+TTGGA
+>phiX174_1673_RIGHT
+GTAAA
+>phiX174_1674_LEFT
+TGGAG
+>phiX174_1674_RIGHT
+TAAAA
+>phiX174_1677_LEFT
+AGGTA
+>phiX174_1677_RIGHT
+AACCT
+>phiX174_1678_LEFT
+GGTAA
+>phiX174_1678_RIGHT
+ACCTC
+>phiX174_1682_LEFT
+AAACC
+>phiX174_1682_RIGHT
+CTTAT
+>phiX174_1685_LEFT
+CCTCT
+>phiX174_1685_RIGHT
+ATGAC
+>phiX174_1686_LEFT
+CTCTT
+>phiX174_1686_RIGHT
+TGACG
+>phiX174_1687_LEFT
+TCTTA
+>phiX174_1687_RIGHT
+GACGC
+>phiX174_1688_LEFT
+CTTAT
+>phiX174_1688_RIGHT
+ACGCT
+>phiX174_1689_LEFT
+TTATG
+>phiX174_1689_RIGHT
+CGCTG
+>phiX174_1691_LEFT
+ATGAC
+>phiX174_1691_RIGHT
+CTGAC
+>phiX174_1693_LEFT
+GACGC
+>phiX174_1693_RIGHT
+GACAA
+>phiX174_1695_LEFT
+CGCTG
+>phiX174_1695_RIGHT
+CAACC
+>phiX174_1702_LEFT
+AACCG
+>phiX174_1702_RIGHT
+CCTTT
+>phiX174_1707_LEFT
+TCCTT
+>phiX174_1707_RIGHT
+ACTTG
+>phiX174_1708_LEFT
+CCTTT
+>phiX174_1708_RIGHT
+CTTGT
+>phiX174_1710_LEFT
+TTTAC
+>phiX174_1710_RIGHT
+TGTCA
+>phiX174_1723_LEFT
+CGCTC
+>phiX174_1723_RIGHT
+AATCT
+>phiX174_1724_LEFT
+GCTCT
+>phiX174_1724_RIGHT
+ATCTC
+>phiX174_1725_LEFT
+CTCTA
+>phiX174_1725_RIGHT
+TCTCT
+>phiX174_1726_LEFT
+TCTAA
+>phiX174_1726_RIGHT
+CTCTG
+>phiX174_1730_LEFT
+ATCTC
+>phiX174_1730_RIGHT
+GGGCA
+>phiX174_1739_LEFT
+CATCT
+>phiX174_1739_RIGHT
+GCTAT
+>phiX174_1746_LEFT
+CTATG
+>phiX174_1746_RIGHT
+TGTTG
+>phiX174_1749_LEFT
+TGATG
+>phiX174_1749_RIGHT
+TGATG
+>phiX174_1755_LEFT
+TGATG
+>phiX174_1755_RIGHT
+AACTG
+>phiX174_1756_LEFT
+GATGG
+>phiX174_1756_RIGHT
+ACTGA
+>phiX174_1761_LEFT
+AACTG
+>phiX174_1761_RIGHT
+CCAAA
+>phiX174_1764_LEFT
+TGACC
+>phiX174_1764_RIGHT
+AACGT
+>phiX174_1766_LEFT
+ACCAA
+>phiX174_1766_RIGHT
+CGTCG
+>phiX174_1769_LEFT
+AAACG
+>phiX174_1769_RIGHT
+CGTTA
+>phiX174_1771_LEFT
+ACGTC
+>phiX174_1771_RIGHT
+TTAGG
+>phiX174_1772_LEFT
+CGTCG
+>phiX174_1772_RIGHT
+TAGGC
+>phiX174_1774_LEFT
+TCGTT
+>phiX174_1774_RIGHT
+GGCCA
+>phiX174_1775_LEFT
+CGTTA
+>phiX174_1775_RIGHT
+GCCAG
+>phiX174_1776_LEFT
+GTTAG
+>phiX174_1776_RIGHT
+CCAGT
+>phiX174_1778_LEFT
+TAGGC
+>phiX174_1778_RIGHT
+AGTTT
+>phiX174_1779_LEFT
+AGGCC
+>phiX174_1779_RIGHT
+GTTTT
+>phiX174_1781_LEFT
+GCCAG
+>phiX174_1781_RIGHT
+TTTCT
+>phiX174_1782_LEFT
+CCAGT
+>phiX174_1782_RIGHT
+TTCTG
+>phiX174_1783_LEFT
+CAGTT
+>phiX174_1783_RIGHT
+TCTGG
+>phiX174_1788_LEFT
+TTCTG
+>phiX174_1788_RIGHT
+TCGTG
+>phiX174_1792_LEFT
+GGTCG
+>phiX174_1792_RIGHT
+GTTCA
+>phiX174_1802_LEFT
+AACAG
+>phiX174_1802_RIGHT
+CCTAT
+>phiX174_1804_LEFT
+CAGAC
+>phiX174_1804_RIGHT
+TATAA
+>phiX174_1805_LEFT
+AGACC
+>phiX174_1805_RIGHT
+ATAAA
+>phiX174_1806_LEFT
+GACCT
+>phiX174_1806_RIGHT
+TAAAC
+>phiX174_1824_LEFT
+GCCGC
+>phiX174_1824_RIGHT
+TTTCT
+>phiX174_1825_LEFT
+CCGCG
+>phiX174_1825_RIGHT
+TTCTT
+>phiX174_1833_LEFT
+CTTTG
+>phiX174_1833_RIGHT
+TCCTG
+>phiX174_1843_LEFT
+GAGCA
+>phiX174_1843_RIGHT
+GGCAC
+>phiX174_1847_LEFT
+ATGGC
+>phiX174_1847_RIGHT
+CTATG
+>phiX174_1854_LEFT
+TATGT
+>phiX174_1854_RIGHT
+TACTC
+>phiX174_1860_LEFT
+TACTC
+>phiX174_1860_RIGHT
+TGCGC
+>phiX174_1863_LEFT
+TCTTG
+>phiX174_1863_RIGHT
+GCTTG
+>phiX174_1866_LEFT
+TGCGC
+>phiX174_1866_RIGHT
+TGTTC
+>phiX174_1875_LEFT
+TCGTT
+>phiX174_1875_RIGHT
+TCCGC
+>phiX174_1876_LEFT
+CGTTT
+>phiX174_1876_RIGHT
+CCGCC
+>phiX174_1882_LEFT
+CCGCC
+>phiX174_1882_RIGHT
+ACTGC
+>phiX174_1889_LEFT
+CTGCG
+>phiX174_1889_RIGHT
+CTAAA
+>phiX174_1913_LEFT
+TTAAC
+>phiX174_1913_RIGHT
+CTAAA
+>phiX174_1915_LEFT
+AACGC
+>phiX174_1915_RIGHT
+AAAGG
+>phiX174_1917_LEFT
+CGCTA
+>phiX174_1917_RIGHT
+AGGTG
+>phiX174_1930_LEFT
+TTGAC
+>phiX174_1930_RIGHT
+TATAC
+>phiX174_1934_LEFT
+CTTAT
+>phiX174_1934_RIGHT
+CCGAT
+>phiX174_1940_LEFT
+CCGAT
+>phiX174_1940_RIGHT
+TTGCT
+>phiX174_1942_LEFT
+GATAT
+>phiX174_1942_RIGHT
+GCTGG
+>phiX174_1945_LEFT
+ATTGC
+>phiX174_1945_RIGHT
+GGCGA
+>phiX174_1954_LEFT
+GACCC
+>phiX174_1954_RIGHT
+GTTTT
+>phiX174_1959_LEFT
+TGTTT
+>phiX174_1959_RIGHT
+GTATG
+>phiX174_1994_LEFT
+CTATG
+>phiX174_1994_RIGHT
+AGGAT
+>phiX174_1998_LEFT
+GAAGG
+>phiX174_1998_RIGHT
+TGTTT
diff --git a/tool_collections/vcflib/vcfprimers/tool-data/fasta_indexes.loc.sample b/tool_collections/vcflib/vcfprimers/tool-data/fasta_indexes.loc.sample
new file mode 120000
index 00000000000..6d7aec6c449
--- /dev/null
+++ b/tool_collections/vcflib/vcfprimers/tool-data/fasta_indexes.loc.sample
@@ -0,0 +1 @@
+../../tool-data/fasta_indexes.loc.sample
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfprimers/tool-data/tool_data_table_conf.xml.sample b/tool_collections/vcflib/vcfprimers/tool-data/tool_data_table_conf.xml.sample
new file mode 120000
index 00000000000..7bfcf015ca6
--- /dev/null
+++ b/tool_collections/vcflib/vcfprimers/tool-data/tool_data_table_conf.xml.sample
@@ -0,0 +1 @@
+../../tool-data/tool_data_table_conf.xml.sample
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfprimers/vcfprimers.xml b/tool_collections/vcflib/vcfprimers/vcfprimers.xml
new file mode 100644
index 00000000000..4402dca04c8
--- /dev/null
+++ b/tool_collections/vcflib/vcfprimers/vcfprimers.xml
@@ -0,0 +1,67 @@
+<tool id="vcfprimers" name="VCFprimers:" version="@WRAPPER_VERSION@.0">
+  <description>Extract flanking sequences for each VCF record</description>
+  <macros>
+    <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"/>
+  <expand macro="stdio" />
+  <command>
+    #set $reference_fasta_filename = "localref.fa"
+    #if str( $reference_source.reference_source_selector ) == "history":
+       ln -s "${reference_source.ref_file}" "${reference_fasta_filename}" &amp;&amp;
+    #else:
+       #set $reference_fasta_filename = str( $reference_source.ref_file.fields.path )
+    #end if    
+   vcfprimers -f "${reference_fasta_filename}" -l "${primer_length}" "${input_vcf}" > "${out_file1}"</command>
+  <inputs>
+<param name="input_vcf" type="data" format="vcf" label="VCF dataset to extract flanks" />
+    <conditional name="reference_source">
+       <param name="reference_source_selector" type="select" label="Choose the source for the reference genome">
+         <option value="cached">Locally cached</option>
+         <option value="history">History</option>
+       </param>
+       <when value="cached">
+         <param name="ref_file" type="select" label="Select reference genome">
+           <options from_data_table="fasta_indexes">
+           </options>
+	   <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/>
+         </param>
+       </when>
+       <when value="history"> <!-- FIX ME!!!! -->
+         <param name="ref_file" type="data" format="fasta" label="Using reference file" />
+       </when>
+     </conditional>
+     <param name="primer_length" type="integer" value="20" label="The length of the primer sequences on each side of the variant" help="default = 20 bp" />
+  </inputs>
+  <outputs>
+    <data format="fasta" name="out_file1" />
+  </outputs>
+  <tests>
+    <test>
+      <param name="reference_source_selector" value="history" />
+      <param name="input_vcf" value="vcflib-phix.vcf"/>
+      <param name="ref_file" value="vcflib-test-genome-phix.fa" />
+      <param name="primer_length" value="5" />
+      <output name="out_file1" file="vcfprimers-test1.fasta"/>
+    </test>
+    </tests>
+  <help>
+
+For each VCF record, extract the flanking sequences, and write them as FASTA
+records suitable for alignment.  This tool is intended for use in designing validation
+experiments.  Primers extracted which would flank all of the alleles at multi-allelic
+sites.  The name of the FASTA "reads" indicates the VCF record which they apply to.
+The form is >CHROM_POS_LEFT for the 3' primer and >CHROM_POS_RIGHT for the 5' primer,
+for example::
+
+ >20_233255_LEFT
+ CCATTGTATATATAGACCATAATTTCTTTATCCAATCATCTGTTGATGGA
+ >20_233255_RIGHT
+ ACTCAGTTGATTCCATACCTTTGCCATCATGAATCATGTTGTAATAAACA
+
+----
+
+Vcfprimers @IS_PART_OF_VCFLIB@
+</help>
+  <expand macro="citations" />
+</tool>
diff --git a/tool_collections/vcflib/vcfrandomsample/.shed.yml b/tool_collections/vcflib/vcfrandomsample/.shed.yml
new file mode 100644
index 00000000000..608bf958db6
--- /dev/null
+++ b/tool_collections/vcflib/vcfrandomsample/.shed.yml
@@ -0,0 +1,12 @@
+categories:
+- Variant Analysis
+description: Randomly sample sites from VCF dataset
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  Randomly sample sites from an input VCF file. Scale the sampling
+  probability by the field specified by --scale-by (see advanced controls).  This may be
+  used to provide uniform sampling across allele frequencies, for instance (AF field in this case).
+name: vcfrandomsample
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfrandomsample
+type: unrestricted
diff --git a/tool_collections/vcflib/vcfrandomsample/macros.xml b/tool_collections/vcflib/vcfrandomsample/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcfrandomsample/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfrandomsample/test-data/vcflib.vcf b/tool_collections/vcflib/vcfrandomsample/test-data/vcflib.vcf
new file mode 100644
index 00000000000..e8dd794d9bd
--- /dev/null
+++ b/tool_collections/vcflib/vcfrandomsample/test-data/vcflib.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	NS=3;DP=14;AF=0.5;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	NS=3;DP=11;AF=0.017	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	NS=2;DP=10;AF=0.333,0.667;AA=T;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	NS=3;DP=13;AA=T	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	NS=3;DP=9;AA=G;AN=6;AC=3,1	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	.	.	.	GT	0/0	0|0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcfrandomsample/test-data/vcfrandomsample-test1.vcf b/tool_collections/vcflib/vcfrandomsample/test-data/vcfrandomsample-test1.vcf
new file mode 100644
index 00000000000..e2a2bd7a0b8
--- /dev/null
+++ b/tool_collections/vcflib/vcfrandomsample/test-data/vcfrandomsample-test1.vcf
@@ -0,0 +1,26 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+##sampling="random sampling using vcfrandomsample -p 1 -r 0.2 /space/anton/galaxy-central/database/files/000/dataset_1.dat using random seed 1"
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+20	1110696	rs6040355	A	G,T	67	PASS	AA=T;AF=0.333,0.667;DP=10;NS=2;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	AA=T;DP=13;NS=3	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcfrandomsample/vcfrandomsample.xml b/tool_collections/vcflib/vcfrandomsample/vcfrandomsample.xml
new file mode 100644
index 00000000000..4ba1ac17b44
--- /dev/null
+++ b/tool_collections/vcflib/vcfrandomsample/vcfrandomsample.xml
@@ -0,0 +1,54 @@
+<tool id="vcfrandomsample" name="VCFrandomSample:" version="@WRAPPER_VERSION@.0">
+    <description>Randomly sample sites from VCF dataset</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"/>
+    <expand macro="stdio" />
+    <command>
+        vcfrandomsample
+            #if $adv_options.adv_options_selector == "use_adv_controls":
+                -s "${adv_options.scale_by}"
+            #end if
+            -p ${random_seed}
+            -r ${rate}
+            "${vcf_input}" > "${out_file1}" 
+    </command>
+    <inputs>
+        <param format="vcf" name="vcf_input" type="data" label="Select random sites from"/>
+        <param name="rate" type="float" value="0.1" label="base sampling probability per locus" help="-r, --rate"/>
+        <param name="random_seed" type="integer" value="17823618326" label="use this random seed"
+            help="-p, --random-seed; use this to make results reproducible" />
+        <conditional name="adv_options">
+            <param name="adv_options_selector" type="select" label="Advanced controls" help="Allows you to specify options that are not listed above">
+                <option value="no">Don't use advanced options</option>
+                <option value="use_adv_controls">Use advanced options</option>
+            </param>
+            <when value="no" />
+            <when value="use_adv_controls">
+                <param name="scale_by" type="text" value="AF" label="scale sampling likelihood by this INFO field" help="-s, --scale-by; this only works for Float info fields such as AF" />
+            </when>
+        </conditional>
+    </inputs>
+    <outputs>
+        <data format="vcf" name="out_file1" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="vcf_input" value="vcflib.vcf"/>
+            <param name="rate" value="0.2" />
+            <param name="random_seed" value="1" />
+            <param name="adv_options_selector" value="no" />
+            <output name="out_file1" file="vcfrandomsample-test1.vcf" lines_diff="2" />
+        </test>
+    </tests>
+    <help>
+Randomly sample sites from an input VCF dataset. Scale the sampling probability by the field specified by --scale-by (see advanced controls).  This may be
+used to provide uniform sampling across allele frequencies, for instance (AF field in this case).
+
+----
+
+Vcfrandomsample @IS_PART_OF_VCFLIB@
+    </help>
+    <expand macro="citations" />
+</tool>
diff --git a/tool_collections/vcflib/vcfselectsamples/.shed.yml b/tool_collections/vcflib/vcfselectsamples/.shed.yml
new file mode 100644
index 00000000000..c55d36bcbc0
--- /dev/null
+++ b/tool_collections/vcflib/vcfselectsamples/.shed.yml
@@ -0,0 +1,11 @@
+categories:
+- Variant Analysis
+description: Select samples from a VCF file
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  Allows selecting samples from a VCF dataset. This tool combines
+  vcfkeepsamples and vcfremovesamples from VCFlib package into a single utility.
+name: vcfselectsamples
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfselectsamples
+type: unrestricted
diff --git a/tool_collections/vcflib/vcfselectsamples/macros.xml b/tool_collections/vcflib/vcfselectsamples/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcfselectsamples/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfselectsamples/test-data/vcflib.vcf b/tool_collections/vcflib/vcfselectsamples/test-data/vcflib.vcf
new file mode 100644
index 00000000000..e8dd794d9bd
--- /dev/null
+++ b/tool_collections/vcflib/vcfselectsamples/test-data/vcflib.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	NS=3;DP=14;AF=0.5;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	NS=3;DP=11;AF=0.017	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	NS=2;DP=10;AF=0.333,0.667;AA=T;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	NS=3;DP=13;AA=T	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	NS=3;DP=9;AA=G;AN=6;AC=3,1	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	.	.	.	GT	0/0	0|0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0/1	0|2
diff --git a/tool_collections/vcflib/vcfselectsamples/test-data/vcfselectsamples-test1.vcf b/tool_collections/vcflib/vcfselectsamples/test-data/vcfselectsamples-test1.vcf
new file mode 100644
index 00000000000..01f2a43aeaa
--- /dev/null
+++ b/tool_collections/vcflib/vcfselectsamples/test-data/vcfselectsamples-test1.vcf
@@ -0,0 +1,31 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	AF=0.5;DP=14;NS=3;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	AF=0.017;DP=11;NS=3	GT:GQ:DP:HQ	0|0:49:3:58,50	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	AA=T;AF=0.333,0.667;DP=10;NS=2;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	AA=T;DP=13;NS=3	GT:GQ:DP:HQ	0|0:54:.:56,60	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	AA=G;AC=3,1;AN=6;DP=9;NS=3	GT:GQ:DP	0/1:.:4	1/1:40:3
+20	1235237	.	T	.	0	.	.	GT	0/0	./.
+X	10	rsTest	AC	A,ATG	10	PASS	.	GT	0	0|2
diff --git a/tool_collections/vcflib/vcfselectsamples/vcfselectsamples.xml b/tool_collections/vcflib/vcfselectsamples/vcfselectsamples.xml
new file mode 100644
index 00000000000..d66dbd537d7
--- /dev/null
+++ b/tool_collections/vcflib/vcfselectsamples/vcfselectsamples.xml
@@ -0,0 +1,53 @@
+<tool id="vcfselectsamples" name="VCFselectsamples:" version="@WRAPPER_VERSION@.0">
+  <description>Select samples from a VCF dataset</description>
+  <macros>
+    <import>macros.xml</import>
+  </macros>
+  <expand macro="requirements"/>
+  <expand macro="stdio" />
+  <command>vcfkeepsamples "${input1}" ${ ' '.join( map( lambda x:'"%s"' % ( x ), str($vcf_samples ).split(",") ) ) } > "${out_file1}"</command>
+  <inputs>
+    <param format="vcf" name="input1" type="data" label="Select VCF dataset"  />
+    <param name="vcf_samples" type="select" label="Check to keep samples; Uncheck to remove" display="checkboxes" multiple="true">
+      <options>
+        <filter type="data_meta" ref="input1" key="sample_names" />
+      </options>
+    </param>
+  </inputs>
+  <outputs>
+    <data format="vcf" name="out_file1" />
+  </outputs>
+  <tests>
+    <test>
+      <param name="vcf_samples" value="NA00001,NA00003"/>
+      <param name="input1" value="vcflib.vcf"/>
+      <output name="out_file1" file="vcfselectsamples-test1.vcf"/>
+    </test>
+   </tests>
+  <help>
+
+Allows to keep or remove samples from a VCF file.
+
+-----
+
+.. class:: infomark
+
+
+**Example**:
+
+Selecting *NA00001* and *NA00003* from the following VCF line::
+
+ #CHROM POS     ID        REF ALT QUAL FILTER INFO                              FORMAT      NA00001        NA00002      NA00003
+ 20     1110696 rs6040355 A   G,T 67   PASS   NS=2;DP=10;AF=0.333,0.667;AA=T;DB GT:GQ:DP:HQ 1|2:21:6:23,27 2|1:2:0:18,2 2/2:35:4:.,.
+
+will, obviously, remove *NA00002*::
+
+ #CHROM POS     ID        REF ALT QUAL FILTER INFO                              FORMAT      NA00001        NA00003                                                  
+ 20     1110696 rs6040355 A   G,T 67   PASS   NS=2;DP=10;AF=0.333,0.667;AA=T;DB GT:GQ:DP:HQ 1|2:21:6:23,27 2/2:35:4:.,.
+
+----
+
+Vcfselectsamples is based on vcfkeepsamples/vcfremovesamples utilities from **VCFlib** toolkit developed by Erik Garrison (https://github.com/ekg/vcflib).
+</help>
+  <expand macro="citations" />
+</tool>
diff --git a/tool_collections/vcflib/vcfsort/.shed.yml b/tool_collections/vcflib/vcfsort/.shed.yml
new file mode 100644
index 00000000000..414bea53b29
--- /dev/null
+++ b/tool_collections/vcflib/vcfsort/.shed.yml
@@ -0,0 +1,10 @@
+categories:
+- Variant Analysis
+description: Sort VCF dataset by coordinate
+long_description: |
+  This tools uses native UNIX sort command to order VCF dataset in coordinate order.
+name: vcfsort
+owner: devteam
+homepage_url: https://github.com/ekg/vcflib
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfsort
+type: unrestricted
diff --git a/tool_collections/vcflib/vcfsort/macros.xml b/tool_collections/vcflib/vcfsort/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcfsort/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfsort/test-data/vcfsort-test1-input.vcf b/tool_collections/vcflib/vcfsort/test-data/vcfsort-test1-input.vcf
new file mode 100644
index 00000000000..22a28296937
--- /dev/null
+++ b/tool_collections/vcflib/vcfsort/test-data/vcfsort-test1-input.vcf
@@ -0,0 +1,30 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+20	14370	rs6054257	G	A	29	PASS	NS=3;DP=14;AF=0.5;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	NS=3;DP=11;AF=0.017	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	NS=2;DP=10;AF=0.333,0.667;AA=T;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	NS=3;DP=13;AA=T	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	NS=3;DP=9;AA=G;AN=6;AC=3,1	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	.	.	.	GT	0/0	0|0	./.
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
diff --git a/tool_collections/vcflib/vcfsort/test-data/vcfsort-test1.vcf b/tool_collections/vcflib/vcfsort/test-data/vcfsort-test1.vcf
new file mode 100644
index 00000000000..e41d940304f
--- /dev/null
+++ b/tool_collections/vcflib/vcfsort/test-data/vcfsort-test1.vcf
@@ -0,0 +1,30 @@
+##fileformat=VCFv4.0
+##fileDate=20090805
+##source=myImputationProgramV3.1
+##reference=1000GenomesPilot-NCBI36
+##phasing=partial
+##INFO=<ID=NS,Number=1,Type=Integer,Description="Number of Samples With Data">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
+##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency">
+##INFO=<ID=AA,Number=1,Type=String,Description="Ancestral Allele">
+##INFO=<ID=DB,Number=0,Type=Flag,Description="dbSNP membership, build 129">
+##INFO=<ID=H2,Number=0,Type=Flag,Description="HapMap2 membership">
+##FILTER=<ID=q10,Description="Quality below 10">
+##FILTER=<ID=s50,Description="Less than 50% of samples have data">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth">
+##FORMAT=<ID=HQ,Number=2,Type=Integer,Description="Haplotype Quality">
+##ALT=<ID=DEL:ME:ALU,Description="Deletion of ALU element">
+##ALT=<ID=CNV,Description="Copy number variable region">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	NA00001	NA00002	NA00003
+19	111	.	A	C	9.6	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+19	112	.	A	G	10	.	.	GT:HQ	0|0:10,10	0|0:10,10	0/1:3,3
+20	14370	rs6054257	G	A	29	PASS	NS=3;DP=14;AF=0.5;DB;H2	GT:GQ:DP:HQ	0|0:48:1:51,51	1|0:48:8:51,51	1/1:43:5:.,.
+20	17330	.	T	A	3	q10	NS=3;DP=11;AF=0.017	GT:GQ:DP:HQ	0|0:49:3:58,50	0|1:3:5:65,3	0/0:41:3:.,.
+20	1110696	rs6040355	A	G,T	67	PASS	NS=2;DP=10;AF=0.333,0.667;AA=T;DB	GT:GQ:DP:HQ	1|2:21:6:23,27	2|1:2:0:18,2	2/2:35:4:.,.
+20	1230237	.	T	.	47	PASS	NS=3;DP=13;AA=T	GT:GQ:DP:HQ	0|0:54:.:56,60	0|0:48:4:51,51	0/0:61:2:.,.
+20	1234567	microsat1	G	GA,GAC	50	PASS	NS=3;DP=9;AA=G;AN=6;AC=3,1	GT:GQ:DP	0/1:.:4	0/2:17:2	1/1:40:3
+20	1235237	.	T	.	.	.	.	GT	0/0	0|0	./.
diff --git a/tool_collections/vcflib/vcfsort/vcfsort.xml b/tool_collections/vcflib/vcfsort/vcfsort.xml
new file mode 100644
index 00000000000..bf66bf24f8a
--- /dev/null
+++ b/tool_collections/vcflib/vcfsort/vcfsort.xml
@@ -0,0 +1,30 @@
+<tool id="vcfsort" name="VCFsort:" version="@WRAPPER_VERSION@.0">
+    <description>Sort VCF dataset by coordinate</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <command><![CDATA[
+(grep ^"#" "${input1}" && grep -v ^"#" "${input1}" | LC_ALL=C sort -k1,1 -k2,2n -V) > "${out_file1}"
+    ]]></command>
+    <inputs>
+        <param format="vcf" name="input1" type="data" label="Select VCF dataset"/>
+    </inputs>
+    <outputs>
+        <data format="vcf" name="out_file1" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="input1" value="vcfsort-test1-input.vcf"/>
+            <output name="out_file1" file="vcfsort-test1.vcf"/>
+        </test>
+    </tests>
+    <help>
+This tool uses native UNIX sort command to order VCF dataset in coordinate order. For technically inclined the command is::
+
+ (grep ^"#" INPUT_file ; grep -v ^"#" INPUT_file | LC_ALL=C sort -k1,1 -k2,2n -V) > OUTPUT_file
+
+.. class:: infomark
+
+The same result can be achieved with the Galaxy's general purpose sort tool (in this case sort on the first and the second column in ascending order).
+    </help>
+</tool>
diff --git a/tool_collections/vcflib/vcfvcfintersect/.shed.yml b/tool_collections/vcflib/vcfvcfintersect/.shed.yml
new file mode 100644
index 00000000000..65835fb4317
--- /dev/null
+++ b/tool_collections/vcflib/vcfvcfintersect/.shed.yml
@@ -0,0 +1,11 @@
+categories:
+- Variant Analysis
+description: Intersect two VCF datasets
+homepage_url: https://github.com/ekg/vcflib
+long_description: |
+  Computes intersections and unions for two VCF datasets. Unifies
+  equivalent alleles within window-size bp.
+name: vcfvcfintersect
+owner: devteam
+remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfvcfintersect
+type: unrestricted
diff --git a/tool_collections/vcflib/vcfvcfintersect/macros.xml b/tool_collections/vcflib/vcfvcfintersect/macros.xml
new file mode 120000
index 00000000000..0c6ff6c3756
--- /dev/null
+++ b/tool_collections/vcflib/vcfvcfintersect/macros.xml
@@ -0,0 +1 @@
+../macros.xml
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfvcfintersect/test-data/vcflib-test-genome-phix.fa b/tool_collections/vcflib/vcfvcfintersect/test-data/vcflib-test-genome-phix.fa
new file mode 100644
index 00000000000..48a6b46678a
--- /dev/null
+++ b/tool_collections/vcflib/vcfvcfintersect/test-data/vcflib-test-genome-phix.fa
@@ -0,0 +1,2 @@
+>phiX174
+GAGTTTTATCGCTTCCATGACGCAGAAGTTAACACTTTCGGATATTTCTGATGAGTCGAAAAATTATCTTGATAAAGCAGGAATTACTACTGCTTGTTTACGAATTAAATCGAAGTGGACTGCTGGCGGAAAATGAGAAAATTCGACCTATCCTTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGTTTAGATATGAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTGGATTACTATCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAGAAATCATGAGTCAAGTTACTGAACAATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTAACCGAAGATGATTTCGATTTTCTGACGAGTAACAAAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCTTGCGTTTATGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTTTATTGCTGCCGTCATTGCTTATTATGTTCATCCCGTCAACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTACGGAAAACATTATTAATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAACACTGACGTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAAATTACGTGCaGAAGGAGTGATGTAATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATTTCAACTACTCCGGTTATCGCTGGCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTTTTTATGTCCCTCATCGTCACGTTTATGGTGAACAGTGGATTAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCCGACTGTTAACACTACTGGTTATATTGACCATGCCGCTTTTCTTGGCACGATTAACCCTGATACCAATAAAATCCCTAAGCATTTGTTTCAGGGTTATTTGAATATCTATAACAACTATTTTAAAGCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATCAAGATGATGCTCGTTATGGTTTCCGTTGCTGCCATCTCAAAAACATTTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGACTTCTACCACATCTATTGACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGCATACTGACCAAGAACGTGATTACTTCATGCAGCGTTACCgTGATGTTATTTCTTCATTTGGAGGTAAAACCTCTTATGACGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCTATGATGTTGATGGAACTGACCAAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAACATTCTGTGCCGCGTTTCTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGCGACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGCTTTGACTTATACCGATATTGCTGGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTGCTGAGGGTCAGTGGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGATTATGACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGAATAGTCAGGTTAAATTTAATGTGACCGTTTATCGCAATCTGCCGACCACTCGCGATTCAATCATGACTTCGTGATAAAAGATTGAGTGTGAGGTTATAACGCCGAAGCGGTAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACCAAGCGAAGCGCGGTAGGTTTTCTGCTTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTCGCCATAATTCAAACTTTTTTTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTTTACAGACACCTAAAGCTACATCGTCAACGTTATATTTTGATAGTTTGACGGTTAATGCTGGTAATGGTGGTTTTCTTCATTGCATTCAGATGGATACATCTGTCAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGCCGACCCTAAATTTTTTGCCTGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTCCCGACTGCCTATGATGTTTATCCTTTGAATGGTCGCCATGATGGTGGTTATTATACCGTCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTACGCCGGGCAATAAtGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGGTGATTTATGTTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAAAGCCGCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACCGATAACAATACTGTAGGCATGGGTGATGCTGGTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCAACTGGACAATCAGAAAGAGATTGCCGAGATGCAAAATGAGACTCAAAAAGAGATTGCTGGCATTCAGTCGGCGACTTCACGCCAGAATACGAAAGACCAGGTATATGCACAAAATGAGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGCGTTGCGTCTATTATGGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAAGCTCAAACGGCTGGTCAGTATTTTACCAATGACCAAATCAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCTCTTCTCATATTGGCGCTACTGCAAAGGATATTTCTAATGTCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTTTTCATGGTATTGATAAAGCTGTTGCCGATACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTCTAATTTGTCTAGGAAATAACCGTCAGGATTGACACCCTCCCAATTGTATGTTTTCATGCCTCCAAATCTTGGAGGCTTTTTTATGGTTCGTTCTTATTACCCTTCTGAATGTCACGCTGATTATTTTGACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAATGCTTGGCTTCCATAAGCAGATGGATAACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAGGGCGTTGAGTTCGATAATGGTGATATGTATGTTGACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTACTGAGAAGTTAATGGATGAATTGGCACAATGCTACAATGTGCTCCCCCAACTTGATATTAATAACACTATAGACCACCGCCCCGAAGGGGACGAAAAATGGTTTTTAGAGAACGAGAAGACGGTTACGCAGTTTTGCCGCAAGCTGGCTGCTGAACGCCCTCTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTaCTATTCAGCGTTTGATGAATGCAATGCGACAGGCTCATGCTGATGGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCTGACGACCGATTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGTGACTATTTTCGTGATATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATGATTCACACGCCGACTGCTATCAGTATTTTTGTGTGCCTGAGTATGGTACAGCTAATGGCCGTCTTCATTTCCATGCGGTGCAtTTTATGCGGACACTTCCTACAGGTAGCGTTGACCCTAATTTTGGTCGTCGGGTACGCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGTGGCCTTATGGTTACAGTATGCCCATCGCAGTTCGCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTAAATACGTTAACAAAAAGTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCACTAAAAACCAAGCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAGAATGAGCCGCAACTTCGGGATGAAAATGCTCACAATGACAAATCTGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGATATTGAAGCAGAACGCAAAAAGAGAGATGAGATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTTTGGCGGCGCAACCTGTGACGACAAATCTGCTCAAATTTATGCGCGCTTCGATAAAAATGATTGGCGTATCCAACCTGCA
diff --git a/tool_collections/vcflib/vcfvcfintersect/test-data/vcfvcfintersect-input1.vcf b/tool_collections/vcflib/vcfvcfintersect/test-data/vcfvcfintersect-input1.vcf
new file mode 100644
index 00000000000..ff8bfd3b023
--- /dev/null
+++ b/tool_collections/vcflib/vcfvcfintersect/test-data/vcfvcfintersect-input1.vcf
@@ -0,0 +1,121 @@
+##fileformat=VCFv4.1
+##fileDate=20140610
+##source=Dan
+##reference=file:///galaxy/data/phiX/sam_index/phiX.fa
+##filter="AF > .001"
+##INFO=<ID=AC,Number=A,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=AF,Number=A,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=AF,Number=.,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=NC,Number=.,Type=String,Description="Nucleotide and indel counts">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	groupA	groupB
+phiX174	1015	.	A	C	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:0,2:0,0.2:A=8,C=2,G=1,	0:0,0:0,0:A=26,G=1,
+phiX174	1016	.	A	T	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:0:0:A=10,	0:2:0.0769230769231:A=24,T=2,
+phiX174	1038	.	A	C	0	.	AC=2;AF=0.046511627907	GT:AC:AF:NC	0:2:0.153846153846:A=11,C=2,	0:0:0:A=30,
+phiX174	1045	.	T	G	0	.	AC=2;AF=0.0425531914894	GT:AC:AF:NC	0:0:0:T=15,G=1,	0:2:0.0625:T=30,G=2,
+phiX174	1048	.	C	A	0	.	AC=2;AF=0.0434782608696	GT:AC:AF:NC	0:2:0.133333333333:A=2,C=13,	0:0:0:C=31,G=1,
+phiX174	1050	.	A	C	0	.	AC=3;AF=0.0714285714286	GT:AC:AF:NC	0:0:0:A=12,	0:3:0.1:A=27,C=3,
+phiX174	1051	.	T	G	0	.	AC=2;AF=0.0487804878049	GT:AC:AF:NC	0:2:0.166666666667:C=1,T=10,G=2,	0:0:0:T=29,G=1,
+phiX174	1054	.	T	G	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:2:0.153846153846:T=11,G=2,	0:0:0:T=31,G=1,
+phiX174	1076	.	G	C	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=2,G=17,	0:0:0:G=36,
+phiX174	1080	.	G	C	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=2,T=1,G=17,	0:0:0:C=1,G=39,
+phiX174	1092	.	T	G	0	.	AC=2;AF=0.0263157894737	GT:AC:AF:NC	0:2:0.0833333333333:C=1,T=22,G=2,	0:0:0:T=52,G=1,
+phiX174	1093	.	T	G	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.08:T=23,G=2,	0:0:0:T=52,
+phiX174	1102	.	T	C	0	.	AC=6;AF=0.0779220779221	GT:AC:AF:NC	0:2:0.0869565217391:C=2,T=21,	0:4:0.0740740740741:C=4,T=50,
+phiX174	1107	.	T	G	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:0:0:T=22,G=1,	0:2:0.04:T=48,G=2,
+phiX174	1110	.	T	C	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:2:0.125:C=2,T=14,	0:0:0:C=1,T=56,G=1,
+phiX174	1121	.	T	G	0	.	AC=3;AF=0.041095890411	GT:AC:AF:NC	0:0:0:T=19,	0:3:0.0555555555556:T=51,G=3,
+phiX174	1124	.	T	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0,2:0,0.117647058824:C=2,T=15,G=1,	0:0,0:0,0:T=48,G=1,
+phiX174	1125	.	T	G	0	.	AC=3;AF=0.0454545454545	GT:AC:AF:NC	0:3:0.176470588235:T=14,G=3,	0:0:0:T=49,G=1,
+phiX174	1131	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0952380952381:T=19,G=2,	0:0:0:T=57,
+phiX174	1141	.	T	G	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0:0:T=20,G=1,	0:2:0.0338983050847:T=57,G=2,
+phiX174	1145	.	G	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.0952380952381:C=2,G=19,	0:0:0:G=52,
+phiX174	1147	.	T	C	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0952380952381:C=2,T=19,	0:0:0:T=50,
+phiX174	1149	.	T	G,C	0	.	AC=2,2;AF=0.027027027027,0.027027027027	GT:AC:AF:NC	0:2,2:0.1,0.1:C=2,T=16,G=2,	0:0,0:0,0:T=54,
+phiX174	1156	.	T	G	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:0:0:C=1,T=15,	0:2:0.0416666666667:T=46,G=2,
+phiX174	1159	.	T	C,G	0	.	AC=2,2;AF=0.0298507462687,0.0298507462687	GT:AC:AF:NC	0:2,0:0.111111111111,0:C=2,T=16,	0:0,2:0,0.0408163265306:C=1,T=47,G=2,
+phiX174	1164	.	T	G	0	.	AC=4;AF=0.0666666666667	GT:AC:AF:NC	0:0:0:C=1,T=16,G=1,	0:4:0.0909090909091:T=40,G=4,
+phiX174	1168	.	T	G	0	.	AC=4;AF=0.0666666666667	GT:AC:AF:NC	0:2:0.0952380952381:C=1,T=19,G=2,	0:2:0.0512820512821:T=37,G=2,
+phiX174	1171	.	T	G	0	.	AC=2;AF=0.0327868852459	GT:AC:AF:NC	0:2:0.0869565217391:T=21,G=2,	0:0:0:T=38,G=1,
+phiX174	1174	.	C	A	0	.	AC=2;AF=0.0327868852459	GT:AC:AF:NC	0:2:0.0869565217391:A=2,C=21,	0:0:0:A=1,C=38,
+phiX174	1178	.	G	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:2:0.0740740740741:C=2,T=1,G=25,	0:0:0:G=38,
+phiX174	1187	.	T	G	0	.	AC=3;AF=0.04	GT:AC:AF:NC	0:3:0.0882352941176:C=1,T=31,G=3,	0:0:0:T=41,
+phiX174	1189	.	T	C	0	.	AC=3;AF=0.0379746835443	GT:AC:AF:NC	0:3:0.0857142857143:C=3,T=32,G=1,	0:0:0:T=44,
+phiX174	1200	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0882352941176:T=31,G=3,	0:0:0:T=48,G=1,
+phiX174	1202	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:0:0:T=32,	0:3:0.06:T=47,G=3,
+phiX174	1205	.	A	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.0588235294118:A=32,C=2,	0:0:0:A=47,C=1,G=1,
+phiX174	1207	.	T	G	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:A=1,T=34,	0:2:0.0425531914894:T=45,G=2,
+phiX174	1214	.	G	A	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:T=1,G=34,	0:2:0.0425531914894:A=2,G=45,
+phiX174	1216	.	C	A	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:C=34,	0:2:0.0425531914894:A=2,C=45,
+phiX174	1217	.	C	A	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.0588235294118:A=2,C=32,	0:0:0:C=46,
+phiX174	1219	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:0:0:C=1,T=33,G=1,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1223	.	C	T	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.0555555555556:C=34,T=2,	0:0:0:C=44,
+phiX174	1227	.	A	C	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0588235294118:A=32,C=2,	0:0:0:A=40,
+phiX174	1232	.	T	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:T=34,	0:2:0.0606060606061:C=1,T=31,G=2,
+phiX174	1236	.	G	T	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0606060606061:A=1,T=2,G=31,	0:0:0:G=35,
+phiX174	1237	.	T	G	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=1,T=33,G=2,	0:0:0:T=33,G=1,
+phiX174	1240	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:0,0:0,0:A=32,T=1,	0:0,2:0,0.0555555555556:A=34,C=2,T=1,
+phiX174	1245	.	G	T	0	.	AC=4;AF=0.0625	GT:AC:AF:NC	0:2:0.0666666666667:C=1,T=2,G=28,	0:2:0.0588235294118:T=2,G=32,
+phiX174	1247	.	A	T	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:2:0.0714285714286:A=26,C=1,T=2,G=1,	0:0:0:A=35,
+phiX174	1250	.	A	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:2:0.0769230769231:A=24,C=2,	0:0:0:A=39,
+phiX174	1251	.	A	G	0	.	AC=3;AF=0.044776119403	GT:AC:AF:NC	0:0:0:A=29,	0:3:0.0789473684211:A=35,C=1,G=3,
+phiX174	1253	.	T	G	0	.	AC=4;AF=0.0526315789474	GT:AC:AF:NC	0:2:0.0645161290323:T=29,G=2,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1257	.	T	G	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:0:0:T=26,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1259	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:A=28,T=1,	0:2:0.0444444444444:A=43,C=2,G=1,
+phiX174	1274	.	G	C	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.0512820512821:C=2,G=37,	0:0:0:G=44,
+phiX174	1277	.	A	C	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=40,G=1,
+phiX174	1279	.	T	G	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0:0:T=39,G=1,	0:2:0.05:C=1,T=38,G=2,
+phiX174	1284	.	T	G,C	0	.	AC=3,2;AF=0.038961038961,0.025974025974	GT:AC:AF:NC	0:3,2:0.075,0.05:C=2,T=35,G=3,	0:0,0:0,0:A=1,T=37,
+phiX174	1289	.	A	C	0	.	AC=3;AF=0.0454545454545	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=28,G=1,
+phiX174	1296	.	A	C	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:0:0:A=38,	0:2:0.0769230769231:A=24,C=2,
+phiX174	1298	.	A	C	0	.	AC=3;AF=0.0491803278689	GT:AC:AF:NC	0:3,0:0.0810810810811,0:A=34,C=3,G=1,	0:0,0:0,0:A=24,C=1,T=1,G=1,
+phiX174	1306	.	T	G	0	.	AC=5;AF=0.0943396226415	GT:AC:AF:NC	0:5:0.178571428571:T=23,G=5,	0:0:0:C=1,T=25,
+phiX174	1307	.	T	G	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.107142857143:T=25,G=3,	0:0:0:C=1,T=27,
+phiX174	1314	.	A	G	0	.	AC=5;AF=0.0877192982456	GT:AC:AF:NC	0:2,0:0.0740740740741,0:A=25,C=1,G=2,	0:3,0:0.1,0:A=27,C=1,G=3,
+phiX174	1317	.	A	C	0	.	AC=2;AF=0.0322580645161	GT:AC:AF:NC	0:2:0.0740740740741:A=25,C=2,	0:0:0:A=35,
+phiX174	1320	.	C	G	0	.	AC=2;AF=0.0322580645161	GT:AC:AF:NC	0:2:0.0769230769231:C=24,G=2,	0:0:0:C=36,
+phiX174	1322	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.107142857143:C=3,G=25,	0:0:0:G=40,
+phiX174	1323	.	C	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:C=28,	0:2:0.0512820512821:C=37,T=1,G=2,
+phiX174	1329	.	T	C	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:2:0.0666666666667:C=2,T=28,	0:0:0:T=42,G=1,
+phiX174	1335	.	C	A	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.0625:A=2,C=30,	0:0:0:C=45,
+phiX174	1338	.	T	A	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:0:0:T=32,	0:2:0.047619047619:A=2,T=40,
+phiX174	1345	.	T	C	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0,0:0,0:T=36,G=1,	0:0,2:0,0.046511627907:C=2,T=41,G=1,
+phiX174	1347	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0512820512821:A=37,C=2,G=1,	0:0:0:A=43,
+phiX174	1348	.	T	G	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.05:T=38,G=2,	0:0:0:T=43,
+phiX174	1352	.	A	T	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:A=34,G=1,	0:2:0.0512820512821:A=37,T=2,
+phiX174	1354	.	T	G,A	0	.	AC=2,2;AF=0.027027027027,0.027027027027	GT:AC:AF:NC	0:2,0:0.0526315789474,0:T=36,G=2,	0:0,2:0,0.0555555555556:A=2,T=34,G=1,
+phiX174	1359	.	T	G	0	.	AC=3;AF=0.0405405405405	GT:AC:AF:NC	0:3:0.0714285714286:T=39,G=3,	0:0:0:T=32,G=1,
+phiX174	1366	.	G	C	0	.	AC=3;AF=0.0428571428571	GT:AC:AF:NC	0:3:0.0714285714286:C=3,T=1,G=39,	0:0:0:G=28,
+phiX174	1370	.	T	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:0:0:C=1,T=45,G=1,	0:2:0.0833333333333:A=1,T=22,G=2,
+phiX174	1373	.	T	G	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0408163265306:T=47,G=2,	0:0:0:T=25,
+phiX174	1380	.	G	C	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:0,2:0,0.0384615384615:C=2,T=1,G=50,	0:0,0:0,0:T=1,G=32,
+phiX174	1381	.	T	G	0	.	AC=3;AF=0.0361445783133	GT:AC:AF:NC	0:3:0.0576923076923:A=1,T=49,G=3,	0:0:0:T=31,
+phiX174	1384	.	T	G	0	.	AC=4;AF=0.0512820512821	GT:AC:AF:NC	0:4:0.0816326530612:T=45,G=4,	0:0:0:T=29,G=1,
+phiX174	1385	.	T	G	0	.	AC=4;AF=0.0512820512821	GT:AC:AF:NC	0:4:0.0816326530612:T=45,G=4,	0:0:0:C=1,T=29,
+phiX174	1391	.	A	C	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:2:0.0408163265306:A=47,C=2,	0:0:0:A=30,C=1,
+phiX174	1393	.	C	A	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:2:0.0425531914894:A=2,C=45,	0:0:0:C=32,
+phiX174	1396	.	T	G	0	.	AC=3;AF=0.04	GT:AC:AF:NC	0:3:0.0697674418605:T=40,G=3,	0:0:0:T=32,
+phiX174	1397	.	A	C	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:0:0:A=42,T=1,	0:2:0.0625:A=30,C=2,
+phiX174	1400	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.047619047619:A=40,C=2,G=1,	0:0:0:A=31,
+phiX174	1401	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.047619047619:A=40,C=2,	0:0:0:A=31,
+phiX174	1434	.	C	T	0	.	AC=2;AF=0.0571428571429	GT:AC:AF:NC	0:2:0.142857142857:C=12,T=2,	0:0:0:C=21,
+phiX174	1438	.	G	C	0	.	AC=2;AF=0.0526315789474	GT:AC:AF:NC	0:0:0:G=15,	0:2:0.0869565217391:C=2,G=21,
+phiX174	1450	.	T	G	0	.	AC=3;AF=0.0789473684211	GT:AC:AF:NC	0:3:0.176470588235:A=1,C=1,T=14,G=3,	0:0:0:T=21,G=1,
+phiX174	1459	.	T	G	0	.	AC=3;AF=0.075	GT:AC:AF:NC	0:3:0.142857142857:C=1,T=18,G=3,	0:0:0:T=19,
+phiX174	1470	.	C	A	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:0:0:C=29,	0:2:0.0869565217391:A=2,C=21,
+phiX174	1484	.	C	G	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:2:0.0740740740741:A=1,C=25,G=2,	0:0:0:C=25,
+phiX174	1487	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0689655172414:T=27,G=2,	0:0:0:T=26,
+phiX174	1491	.	G	T	0	.	AC=2;AF=0.037037037037	GT:AC:AF:NC	0:0:0:T=1,G=26,	0:2:0.0714285714286:T=2,G=26,
+phiX174	1503	.	A	C	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:0:0:A=20,C=1,	0:2:0.0833333333333:A=22,C=2,
+phiX174	1536	.	A	C	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.111111111111:A=24,C=3,	0:0:0:A=28,C=1,
+phiX174	1541	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0740740740741:T=25,G=2,	0:0:0:T=28,
+phiX174	1553	.	A	G	0	.	AC=2;AF=0.0408163265306	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=1,G=2,	0:0:0:A=28,
+phiX174	1566	.	C	G	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0:0:C=30,T=1,	0:2:0.0571428571429:C=33,G=2,
+phiX174	1572	.	T	G	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:2:0.0625:T=30,G=2,	0:0:0:T=32,
+phiX174	1574	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0882352941176:C=3,G=31,	0:0:0:G=34,
+phiX174	1575	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=33,T=1,
+phiX174	1577	.	A	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:0:0:A=35,	0:3:0.0909090909091:A=30,C=3,
+phiX174	1581	.	T	G	0	.	AC=2;AF=0.0285714285714	GT:AC:AF:NC	0:2:0.0555555555556:C=1,T=34,G=2,	0:0:0:T=34,G=1,
+phiX174	1591	.	A	C	0	.	AC=3;AF=0.037037037037	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=43,C=1,
+phiX174	1597	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0606060606061:T=31,G=2,	0:0:0:C=1,T=45,
diff --git a/tool_collections/vcflib/vcfvcfintersect/test-data/vcfvcfintersect-input2.vcf b/tool_collections/vcflib/vcfvcfintersect/test-data/vcfvcfintersect-input2.vcf
new file mode 100644
index 00000000000..3e204c5b440
--- /dev/null
+++ b/tool_collections/vcflib/vcfvcfintersect/test-data/vcfvcfintersect-input2.vcf
@@ -0,0 +1,122 @@
+##fileformat=VCFv4.1
+##fileDate=20140610
+##source=Dan
+##reference=file:///galaxy/data/phiX/sam_index/phiX.fa
+##filter="AF > .001"
+##INFO=<ID=AC,Number=A,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=AF,Number=A,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=AF,Number=.,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=NC,Number=.,Type=String,Description="Nucleotide and indel counts">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	groupA	groupB
+phiX174	1503	.	A	C	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:0:0:A=20,C=1,	0:2:0.0833333333333:A=22,C=2,
+phiX174	1536	.	A	C	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.111111111111:A=24,C=3,	0:0:0:A=28,C=1,
+phiX174	1541	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0740740740741:T=25,G=2,	0:0:0:T=28,
+phiX174	1553	.	A	G	0	.	AC=2;AF=0.0408163265306	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=1,G=2,	0:0:0:A=28,
+phiX174	1566	.	C	G	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0:0:C=30,T=1,	0:2:0.0571428571429:C=33,G=2,
+phiX174	1572	.	T	G	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:2:0.0625:T=30,G=2,	0:0:0:T=32,
+phiX174	1574	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0882352941176:C=3,G=31,	0:0:0:G=34,
+phiX174	1575	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=33,T=1,
+phiX174	1577	.	A	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:0:0:A=35,	0:3:0.0909090909091:A=30,C=3,
+phiX174	1581	.	T	G	0	.	AC=2;AF=0.0285714285714	GT:AC:AF:NC	0:2:0.0555555555556:C=1,T=34,G=2,	0:0:0:T=34,G=1,
+phiX174	1591	.	A	C	0	.	AC=3;AF=0.037037037037	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=43,C=1,
+phiX174	1597	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0606060606061:T=31,G=2,	0:0:0:C=1,T=45,
+phiX174	1603	.	T	G	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0625:T=30,G=2,	0:0:0:T=50,
+phiX174	1606	.	T	G	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0666666666667:A=1,C=1,T=28,G=2,	0:0:0:T=52,
+phiX174	1611	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0769230769231:A=24,C=2,	0:0:0:A=56,C=1,
+phiX174	1613	.	A	G	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:0:0:A=25,C=1,G=1,	0:2:0.0344827586207:A=56,G=2,
+phiX174	1618	.	C	A	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0666666666667:A=2,C=28,G=1,	0:0:0:A=1,C=57,
+phiX174	1619	.	C	A	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0666666666667:A=2,C=28,	0:0:0:C=57,
+phiX174	1621	.	A	C	0	.	AC=3;AF=0.0352941176471	GT:AC:AF:NC	0:0:0:A=31,T=1,G=1,	0:3:0.0555555555556:A=51,C=3,
+phiX174	1622	.	G	T	0	.	AC=2;AF=0.0224719101124	GT:AC:AF:NC	0:0:0:A=1,G=35,	0:2:0.037037037037:T=2,G=52,
+phiX174	1624	.	A	G	0	.	AC=2;AF=0.0235294117647	GT:AC:AF:NC	0:2:0.0555555555556:A=34,T=1,G=2,	0:0:0:A=49,
+phiX174	1627	.	T	G,C	0	.	AC=2,2;AF=0.0246913580247,0.0246913580247	GT:AC:AF:NC	0:0,0:0,0:T=37,G=1,	0:2,2:0.0454545454545,0.0454545454545:C=2,T=40,G=2,
+phiX174	1630	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0526315789474:C=1,T=36,G=2,	0:0:0:T=40,
+phiX174	1632	.	A	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=41,
+phiX174	1634	.	T	G	0	.	AC=4;AF=0.0493827160494	GT:AC:AF:NC	0:2:0.047619047619:C=1,T=40,G=2,	0:2:0.0512820512821:T=37,G=2,
+phiX174	1635	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0714285714286:T=39,G=3,	0:0:0:T=40,
+phiX174	1639	.	G	A	0	.	AC=3;AF=0.0405405405405	GT:AC:AF:NC	0:3:0.075:A=3,T=1,G=37,	0:0:0:G=34,
+phiX174	1645	.	T	G	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0425531914894:T=45,G=2,	0:0:0:T=27,G=1,
+phiX174	1647	.	A	T,G,C	0	.	AC=2,2,2;AF=0.0253164556962,0.0253164556962,0.0253164556962	GT:AC:AF:NC	0:0,2,2:0,0.0384615384615,0.0384615384615:A=48,C=2,G=2,	0:2,0,0:0.0740740740741,0,0:A=25,T=2,
+phiX174	1652	.	G	A	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.037037037037:A=2,G=52,	0:0:0:G=29,
+phiX174	1656	.	T	G	0	.	AC=3;AF=0.0348837209302	GT:AC:AF:NC	0:3:0.0555555555556:T=51,G=3,	0:0:0:T=32,G=1,
+phiX174	1658	.	A	C	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.04:A=48,C=2,	0:0:0:A=33,
+phiX174	1659	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0625:C=1,T=45,G=3,	0:0:0:T=34,
+phiX174	1663	.	T	G	0	.	AC=4;AF=0.0454545454545	GT:AC:AF:NC	0:0:0:A=1,T=53,	0:4:0.114285714286:T=31,G=4,
+phiX174	1665	.	C	A	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0384615384615:A=2,C=50,G=1,	0:0:0:C=42,
+phiX174	1670	.	G	C	0	.	AC=2;AF=0.0224719101124	GT:AC:AF:NC	0:0,2:0,0.0408163265306:A=1,C=2,T=1,G=47,	0:0,0:0,0:T=1,G=40,
+phiX174	1671	.	G	T	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0384615384615:C=1,T=2,G=50,	0:0:0:G=42,
+phiX174	1672	.	A	G	0	.	AC=3;AF=0.0315789473684	GT:AC:AF:NC	0:3:0.0545454545455:A=52,G=3,	0:0:0:A=40,T=1,
+phiX174	1673	.	G	T	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0377358490566:C=1,T=2,G=51,	0:0:0:G=41,
+phiX174	1674	.	G	T	0	.	AC=4;AF=0.04	GT:AC:AF:NC	0:4:0.0714285714286:T=4,G=52,	0:0:0:G=44,
+phiX174	1677	.	A	C	0	.	AC=3;AF=0.031914893617	GT:AC:AF:NC	0:3:0.0566037735849:A=50,C=3,N=1,	0:0:0:A=41,
+phiX174	1678	.	A	C	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:0:0:A=54,C=1,G=1,	0:2:0.05:A=38,C=2,
+phiX174	1682	.	T	C	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0408163265306:C=2,T=47,G=1,	0:0:0:C=1,T=38,
+phiX174	1685	.	T	G	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:2:0.046511627907:A=1,T=41,G=2,	0:0:0:T=41,G=1,
+phiX174	1686	.	A	C	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:0:0:A=39,C=1,T=1,G=1,	0:2:0.0512820512821:A=37,C=2,
+phiX174	1687	.	T	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.047619047619:C=2,T=40,	0:0:0:A=1,T=40,
+phiX174	1688	.	G	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.05:C=2,T=1,G=38,	0:0:0:G=41,
+phiX174	1689	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0487804878049:A=39,C=2,T=1,G=1,	0:0:0:A=41,C=1,
+phiX174	1691	.	G	C	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.05:C=2,T=1,G=38,	0:0:0:G=37,
+phiX174	1693	.	T	G	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:0:0:T=42,G=1,	0:2:0.047619047619:T=40,G=2,
+phiX174	1695	.	A	C	0	.	AC=2;AF=0.0232558139535	GT:AC:AF:NC	0:2:0.046511627907:A=41,C=2,	0:0:0:A=43,C=1,
+phiX174	1702	.	T	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.0512820512821:A=1,C=2,T=37,	0:0:0:C=1,T=42,
+phiX174	1707	.	T	C	0	.	AC=2;AF=0.0232558139535	GT:AC:AF:NC	0:2:0.05:C=2,T=38,	0:0:0:T=46,G=1,
+phiX174	1708	.	A	C	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:2:0.0526315789474:A=36,C=2,	0:0:0:A=46,C=1,G=1,
+phiX174	1710	.	T	C	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:0:0:C=1,T=34,	0:2:0.0434782608696:C=2,T=44,
+phiX174	1723	.	T	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0588235294118:C=2,T=32,	0:0:0:T=48,
+phiX174	1724	.	A	C	0	.	AC=4;AF=0.0481927710843	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:2:0.0416666666667:A=46,C=2,
+phiX174	1725	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0606060606061:A=31,C=2,	0:0:0:A=49,
+phiX174	1726	.	T	G	0	.	AC=3;AF=0.037037037037	GT:AC:AF:NC	0:3:0.0882352941176:T=31,G=3,	0:0:0:C=1,T=47,
+phiX174	1730	.	T	G	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:C=1,T=28,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1739	.	G	T	0	.	AC=3;AF=0.0434782608696	GT:AC:AF:NC	0:3:0.09375:T=3,G=29,	0:0:0:G=37,
+phiX174	1746	.	A	C	0	.	AC=2;AF=0.0338983050847	GT:AC:AF:NC	0:2:0.0689655172414:A=27,C=2,	0:0:0:A=30,C=1,G=1,
+phiX174	1749	.	T	G,C	0	.	AC=3,2;AF=0.044776119403,0.0298507462687	GT:AC:AF:NC	0:3,2:0.0857142857143,0.0571428571429:C=2,T=30,G=3,	0:0,0:0,0:T=32,G=1,
+phiX174	1755	.	G	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.05:C=2,G=38,	0:0:0:C=1,G=33,
+phiX174	1756	.	A	G,C	0	.	AC=2,2;AF=0.0281690140845,0.0281690140845	GT:AC:AF:NC	0:0,2:0,0.0526315789474:A=36,C=2,T=1,G=1,	0:2,0:0.0606060606061,0:A=31,C=1,G=2,
+phiX174	1761	.	A	G	0	.	AC=2;AF=0.0285714285714	GT:AC:AF:NC	0:2:0.0512820512821:A=37,C=1,G=2,	0:0:0:A=31,
+phiX174	1764	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=28,
+phiX174	1766	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0487804878049:A=39,C=2,	0:0:0:A=27,
+phiX174	1769	.	T	G	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:2:0.0526315789474:A=1,C=1,T=36,G=2,	0:0:0:T=26,G=1,
+phiX174	1771	.	G	C	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:2:0.0526315789474:C=2,G=36,	0:0:0:G=29,
+phiX174	1772	.	T	G	0	.	AC=4;AF=0.0597014925373	GT:AC:AF:NC	0:4:0.108108108108:C=1,T=33,G=4,	0:0:0:T=30,
+phiX174	1774	.	A	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0810810810811:A=34,G=3,	0:0:0:A=31,
+phiX174	1775	.	G	A	0	.	AC=2;AF=0.030303030303	GT:AC:AF:NC	0:2:0.0571428571429:A=2,G=33,	0:0:0:G=31,
+phiX174	1776	.	G	T	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0540540540541:T=2,G=35,	0:0:0:G=32,
+phiX174	1778	.	C	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0526315789474:C=36,G=2,	0:0:0:C=31,
+phiX174	1779	.	A	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.075:A=37,C=3,	0:0:0:A=28,
+phiX174	1781	.	T	G	0	.	AC=5;AF=0.0714285714286	GT:AC:AF:NC	0:2:0.0487804878049:C=1,T=39,G=2,	0:3:0.103448275862:T=26,G=3,
+phiX174	1782	.	T	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.075:T=37,G=3,	0:0:0:A=1,T=28,
+phiX174	1783	.	T	C	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:2:0.0512820512821:C=2,T=37,	0:0:0:T=28,G=1,
+phiX174	1788	.	G	T	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:0:0:A=1,T=1,G=29,	0:2:0.0689655172414:T=2,G=27,
+phiX174	1792	.	T	G	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:2:0.0606060606061:T=31,G=2,	0:0:0:T=25,G=1,
+phiX174	1802	.	A	C	0	.	AC=2;AF=0.030303030303	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=31,C=1,
+phiX174	1804	.	C	T	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0588235294118:A=1,C=32,T=2,	0:0:0:C=34,
+phiX174	1805	.	T	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0857142857143:T=32,G=3,	0:0:0:T=33,G=1,
+phiX174	1806	.	A	C	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=34,G=1,
+phiX174	1824	.	G	C	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0714285714286:C=2,T=1,G=26,	0:0:0:G=43,
+phiX174	1825	.	T	G	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0689655172414:T=27,G=2,	0:0:0:T=42,G=1,
+phiX174	1833	.	T	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:0:0:A=1,T=24,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1843	.	T	G	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:2:0.08:A=1,T=23,G=2,	0:0:0:T=38,
+phiX174	1847	.	A	C	0	.	AC=2;AF=0.0333333333333	GT:AC:AF:NC	0:2:0.0833333333333:A=22,C=2,T=1,G=1,	0:0:0:A=36,
+phiX174	1854	.	T	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:T=26,	0:2:0.0487804878049:T=39,G=2,
+phiX174	1860	.	T	G	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:0:0:A=1,T=20,	0:2:0.0606060606061:T=31,G=2,
+phiX174	1863	.	C	G	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=17,G=2,	0:0:0:C=33,
+phiX174	1866	.	T	G	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:0:0:T=19,G=1,	0:2:0.0645161290323:T=29,G=2,
+phiX174	1875	.	T	A	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:2:0.0952380952381:A=2,T=19,	0:0:0:T=32,G=1,
+phiX174	1876	.	T	G	0	.	AC=3;AF=0.0588235294118	GT:AC:AF:NC	0:3:0.157894736842:C=1,T=16,G=3,	0:0:0:T=32,G=1,
+phiX174	1882	.	T	G	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:2:0.105263157895:T=17,G=2,	0:0:0:T=34,
+phiX174	1889	.	A	C	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:2:0.0869565217391:A=21,C=2,	0:0:0:A=27,
+phiX174	1913	.	G	C	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:0:0:G=27,	0:2:0.0869565217391:C=2,G=21,
+phiX174	1915	.	T	G	0	.	AC=2;AF=0.0425531914894	GT:AC:AF:NC	0:0:0:A=1,T=24,	0:2:0.0869565217391:T=21,G=2,
+phiX174	1917	.	A	T	0	.	AC=2;AF=0.0444444444444	GT:AC:AF:NC	0:2,0:0.0833333333333,0:A=22,T=2,G=1,	0:0,0:0,0:A=21,G=1,
+phiX174	1930	.	T	A	0	.	AC=2;AF=0.0526315789474	GT:AC:AF:NC	0:2:0.0909090909091:A=2,T=20,	0:0:0:T=16,
+phiX174	1934	.	A	C	0	.	AC=2;AF=0.0512820512821	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=2,T=1,	0:0:0:A=18,C=1,
+phiX174	1940	.	A	C	0	.	AC=2;AF=0.046511627907	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=2,	0:0:0:A=22,C=1,
+phiX174	1942	.	T	G	0	.	AC=2;AF=0.047619047619	GT:AC:AF:NC	0:0:0:T=19,	0:2:0.0869565217391:T=21,G=2,
+phiX174	1945	.	T	G	0	.	AC=3;AF=0.0769230769231	GT:AC:AF:NC	0:0:0:T=15,G=1,	0:3:0.125:T=21,G=3,
+phiX174	1954	.	T	C	0	.	AC=2;AF=0.0625	GT:AC:AF:NC	0:0,0:0,0:T=12,G=1,	0:0,2:0,0.1:C=2,T=18,G=1,
+phiX174	1959	.	T	G	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:2:0.153846153846:T=11,G=2,	0:0:0:T=23,
+phiX174	1994	.	A	C	0	.	AC=2;AF=0.0512820512821	GT:AC:AF:NC	0:2:0.153846153846:A=11,C=2,	0:0:0:A=26,
+phiX174	1998	.	A	G	0	.	AC=2;AF=0.05	GT:AC:AF:NC	0:2:0.142857142857:A=12,G=2,	0:0:0:A=26,
diff --git a/tool_collections/vcflib/vcfvcfintersect/test-data/vcfvcfintersect-test1.vcf b/tool_collections/vcflib/vcfvcfintersect/test-data/vcfvcfintersect-test1.vcf
new file mode 100644
index 00000000000..ac48f7398d2
--- /dev/null
+++ b/tool_collections/vcflib/vcfvcfintersect/test-data/vcfvcfintersect-test1.vcf
@@ -0,0 +1,24 @@
+##fileformat=VCFv4.1
+##fileDate=20140610
+##source=Dan
+##reference=file:///galaxy/data/phiX/sam_index/phiX.fa
+##filter="AF > .001"
+##INFO=<ID=AC,Number=A,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=AF,Number=A,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=AF,Number=.,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=NC,Number=.,Type=String,Description="Nucleotide and indel counts">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	groupA	groupB
+phiX174	1503	.	A	C	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:0:0:A=20,C=1,	0:2:0.0833333333333:A=22,C=2,
+phiX174	1536	.	A	C	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.111111111111:A=24,C=3,	0:0:0:A=28,C=1,
+phiX174	1541	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0740740740741:T=25,G=2,	0:0:0:T=28,
+phiX174	1553	.	A	G	0	.	AC=2;AF=0.0408163265306	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=1,G=2,	0:0:0:A=28,
+phiX174	1566	.	C	G	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0:0:C=30,T=1,	0:2:0.0571428571429:C=33,G=2,
+phiX174	1572	.	T	G	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:2:0.0625:T=30,G=2,	0:0:0:T=32,
+phiX174	1574	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0882352941176:C=3,G=31,	0:0:0:G=34,
+phiX174	1575	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=33,T=1,
+phiX174	1577	.	A	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:0:0:A=35,	0:3:0.0909090909091:A=30,C=3,
+phiX174	1581	.	T	G	0	.	AC=2;AF=0.0285714285714	GT:AC:AF:NC	0:2:0.0555555555556:C=1,T=34,G=2,	0:0:0:T=34,G=1,
+phiX174	1591	.	A	C	0	.	AC=3;AF=0.037037037037	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=43,C=1,
+phiX174	1597	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0606060606061:T=31,G=2,	0:0:0:C=1,T=45,
diff --git a/tool_collections/vcflib/vcfvcfintersect/test-data/vcfvcfintersect-test2.vcf b/tool_collections/vcflib/vcfvcfintersect/test-data/vcfvcfintersect-test2.vcf
new file mode 100644
index 00000000000..362e6be2088
--- /dev/null
+++ b/tool_collections/vcflib/vcfvcfintersect/test-data/vcfvcfintersect-test2.vcf
@@ -0,0 +1,219 @@
+##fileformat=VCFv4.1
+##fileDate=20140610
+##source=Dan
+##reference=file:///galaxy/data/phiX/sam_index/phiX.fa
+##filter="AF > .001"
+##INFO=<ID=AC,Number=A,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##INFO=<ID=AF,Number=A,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=AC,Number=.,Type=Integer,Description="Allele count in genotypes, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=AF,Number=.,Type=Float,Description="Allele Frequency, for each ALT allele, in the same order as listed">
+##FORMAT=<ID=NC,Number=.,Type=String,Description="Nucleotide and indel counts">
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	groupA	groupB
+phiX174	1015	.	A	C	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:0,2:0,0.2:A=8,C=2,G=1,	0:0,0:0,0:A=26,G=1,
+phiX174	1016	.	A	T	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:0:0:A=10,	0:2:0.0769230769231:A=24,T=2,
+phiX174	1038	.	A	C	0	.	AC=2;AF=0.046511627907	GT:AC:AF:NC	0:2:0.153846153846:A=11,C=2,	0:0:0:A=30,
+phiX174	1045	.	T	G	0	.	AC=2;AF=0.0425531914894	GT:AC:AF:NC	0:0:0:T=15,G=1,	0:2:0.0625:T=30,G=2,
+phiX174	1048	.	C	A	0	.	AC=2;AF=0.0434782608696	GT:AC:AF:NC	0:2:0.133333333333:A=2,C=13,	0:0:0:C=31,G=1,
+phiX174	1050	.	A	C	0	.	AC=3;AF=0.0714285714286	GT:AC:AF:NC	0:0:0:A=12,	0:3:0.1:A=27,C=3,
+phiX174	1051	.	T	G	0	.	AC=2;AF=0.0487804878049	GT:AC:AF:NC	0:2:0.166666666667:C=1,T=10,G=2,	0:0:0:T=29,G=1,
+phiX174	1054	.	T	G	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:2:0.153846153846:T=11,G=2,	0:0:0:T=31,G=1,
+phiX174	1076	.	G	C	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=2,G=17,	0:0:0:G=36,
+phiX174	1080	.	G	C	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=2,T=1,G=17,	0:0:0:C=1,G=39,
+phiX174	1092	.	T	G	0	.	AC=2;AF=0.0263157894737	GT:AC:AF:NC	0:2:0.0833333333333:C=1,T=22,G=2,	0:0:0:T=52,G=1,
+phiX174	1093	.	T	G	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.08:T=23,G=2,	0:0:0:T=52,
+phiX174	1102	.	T	C	0	.	AC=6;AF=0.0779220779221	GT:AC:AF:NC	0:2:0.0869565217391:C=2,T=21,	0:4:0.0740740740741:C=4,T=50,
+phiX174	1107	.	T	G	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:0:0:T=22,G=1,	0:2:0.04:T=48,G=2,
+phiX174	1110	.	T	C	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:2:0.125:C=2,T=14,	0:0:0:C=1,T=56,G=1,
+phiX174	1121	.	T	G	0	.	AC=3;AF=0.041095890411	GT:AC:AF:NC	0:0:0:T=19,	0:3:0.0555555555556:T=51,G=3,
+phiX174	1124	.	T	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0,2:0,0.117647058824:C=2,T=15,G=1,	0:0,0:0,0:T=48,G=1,
+phiX174	1125	.	T	G	0	.	AC=3;AF=0.0454545454545	GT:AC:AF:NC	0:3:0.176470588235:T=14,G=3,	0:0:0:T=49,G=1,
+phiX174	1131	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0952380952381:T=19,G=2,	0:0:0:T=57,
+phiX174	1141	.	T	G	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0:0:T=20,G=1,	0:2:0.0338983050847:T=57,G=2,
+phiX174	1145	.	G	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.0952380952381:C=2,G=19,	0:0:0:G=52,
+phiX174	1147	.	T	C	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0952380952381:C=2,T=19,	0:0:0:T=50,
+phiX174	1149	.	T	G,C	0	.	AC=2,2;AF=0.027027027027,0.027027027027	GT:AC:AF:NC	0:2,2:0.1,0.1:C=2,T=16,G=2,	0:0,0:0,0:T=54,
+phiX174	1156	.	T	G	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:0:0:C=1,T=15,	0:2:0.0416666666667:T=46,G=2,
+phiX174	1159	.	T	C,G	0	.	AC=2,2;AF=0.0298507462687,0.0298507462687	GT:AC:AF:NC	0:2,0:0.111111111111,0:C=2,T=16,	0:0,2:0,0.0408163265306:C=1,T=47,G=2,
+phiX174	1164	.	T	G	0	.	AC=4;AF=0.0666666666667	GT:AC:AF:NC	0:0:0:C=1,T=16,G=1,	0:4:0.0909090909091:T=40,G=4,
+phiX174	1168	.	T	G	0	.	AC=4;AF=0.0666666666667	GT:AC:AF:NC	0:2:0.0952380952381:C=1,T=19,G=2,	0:2:0.0512820512821:T=37,G=2,
+phiX174	1171	.	T	G	0	.	AC=2;AF=0.0327868852459	GT:AC:AF:NC	0:2:0.0869565217391:T=21,G=2,	0:0:0:T=38,G=1,
+phiX174	1174	.	C	A	0	.	AC=2;AF=0.0327868852459	GT:AC:AF:NC	0:2:0.0869565217391:A=2,C=21,	0:0:0:A=1,C=38,
+phiX174	1178	.	G	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:2:0.0740740740741:C=2,T=1,G=25,	0:0:0:G=38,
+phiX174	1187	.	T	G	0	.	AC=3;AF=0.04	GT:AC:AF:NC	0:3:0.0882352941176:C=1,T=31,G=3,	0:0:0:T=41,
+phiX174	1189	.	T	C	0	.	AC=3;AF=0.0379746835443	GT:AC:AF:NC	0:3:0.0857142857143:C=3,T=32,G=1,	0:0:0:T=44,
+phiX174	1200	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0882352941176:T=31,G=3,	0:0:0:T=48,G=1,
+phiX174	1202	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:0:0:T=32,	0:3:0.06:T=47,G=3,
+phiX174	1205	.	A	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.0588235294118:A=32,C=2,	0:0:0:A=47,C=1,G=1,
+phiX174	1207	.	T	G	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:A=1,T=34,	0:2:0.0425531914894:T=45,G=2,
+phiX174	1214	.	G	A	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:T=1,G=34,	0:2:0.0425531914894:A=2,G=45,
+phiX174	1216	.	C	A	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:0:0:C=34,	0:2:0.0425531914894:A=2,C=45,
+phiX174	1217	.	C	A	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.0588235294118:A=2,C=32,	0:0:0:C=46,
+phiX174	1219	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:0:0:C=1,T=33,G=1,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1223	.	C	T	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.0555555555556:C=34,T=2,	0:0:0:C=44,
+phiX174	1227	.	A	C	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0588235294118:A=32,C=2,	0:0:0:A=40,
+phiX174	1232	.	T	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:T=34,	0:2:0.0606060606061:C=1,T=31,G=2,
+phiX174	1236	.	G	T	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0606060606061:A=1,T=2,G=31,	0:0:0:G=35,
+phiX174	1237	.	T	G	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=1,T=33,G=2,	0:0:0:T=33,G=1,
+phiX174	1240	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:0,0:0,0:A=32,T=1,	0:0,2:0,0.0555555555556:A=34,C=2,T=1,
+phiX174	1245	.	G	T	0	.	AC=4;AF=0.0625	GT:AC:AF:NC	0:2:0.0666666666667:C=1,T=2,G=28,	0:2:0.0588235294118:T=2,G=32,
+phiX174	1247	.	A	T	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:2:0.0714285714286:A=26,C=1,T=2,G=1,	0:0:0:A=35,
+phiX174	1250	.	A	C	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:2:0.0769230769231:A=24,C=2,	0:0:0:A=39,
+phiX174	1251	.	A	G	0	.	AC=3;AF=0.044776119403	GT:AC:AF:NC	0:0:0:A=29,	0:3:0.0789473684211:A=35,C=1,G=3,
+phiX174	1253	.	T	G	0	.	AC=4;AF=0.0526315789474	GT:AC:AF:NC	0:2:0.0645161290323:T=29,G=2,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1257	.	T	G	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:0:0:T=26,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1259	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:A=28,T=1,	0:2:0.0444444444444:A=43,C=2,G=1,
+phiX174	1274	.	G	C	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.0512820512821:C=2,G=37,	0:0:0:G=44,
+phiX174	1277	.	A	C	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=40,G=1,
+phiX174	1279	.	T	G	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0:0:T=39,G=1,	0:2:0.05:C=1,T=38,G=2,
+phiX174	1284	.	T	G,C	0	.	AC=3,2;AF=0.038961038961,0.025974025974	GT:AC:AF:NC	0:3,2:0.075,0.05:C=2,T=35,G=3,	0:0,0:0,0:A=1,T=37,
+phiX174	1289	.	A	C	0	.	AC=3;AF=0.0454545454545	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=28,G=1,
+phiX174	1296	.	A	C	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:0:0:A=38,	0:2:0.0769230769231:A=24,C=2,
+phiX174	1298	.	A	C	0	.	AC=3;AF=0.0491803278689	GT:AC:AF:NC	0:3,0:0.0810810810811,0:A=34,C=3,G=1,	0:0,0:0,0:A=24,C=1,T=1,G=1,
+phiX174	1306	.	T	G	0	.	AC=5;AF=0.0943396226415	GT:AC:AF:NC	0:5:0.178571428571:T=23,G=5,	0:0:0:C=1,T=25,
+phiX174	1307	.	T	G	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.107142857143:T=25,G=3,	0:0:0:C=1,T=27,
+phiX174	1314	.	A	G	0	.	AC=5;AF=0.0877192982456	GT:AC:AF:NC	0:2,0:0.0740740740741,0:A=25,C=1,G=2,	0:3,0:0.1,0:A=27,C=1,G=3,
+phiX174	1317	.	A	C	0	.	AC=2;AF=0.0322580645161	GT:AC:AF:NC	0:2:0.0740740740741:A=25,C=2,	0:0:0:A=35,
+phiX174	1320	.	C	G	0	.	AC=2;AF=0.0322580645161	GT:AC:AF:NC	0:2:0.0769230769231:C=24,G=2,	0:0:0:C=36,
+phiX174	1322	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.107142857143:C=3,G=25,	0:0:0:G=40,
+phiX174	1323	.	C	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:C=28,	0:2:0.0512820512821:C=37,T=1,G=2,
+phiX174	1329	.	T	C	0	.	AC=2;AF=0.0277777777778	GT:AC:AF:NC	0:2:0.0666666666667:C=2,T=28,	0:0:0:T=42,G=1,
+phiX174	1335	.	C	A	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.0625:A=2,C=30,	0:0:0:C=45,
+phiX174	1338	.	T	A	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:0:0:T=32,	0:2:0.047619047619:A=2,T=40,
+phiX174	1345	.	T	C	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:0,0:0,0:T=36,G=1,	0:0,2:0,0.046511627907:C=2,T=41,G=1,
+phiX174	1347	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0512820512821:A=37,C=2,G=1,	0:0:0:A=43,
+phiX174	1348	.	T	G	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.05:T=38,G=2,	0:0:0:T=43,
+phiX174	1352	.	A	T	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:A=34,G=1,	0:2:0.0512820512821:A=37,T=2,
+phiX174	1354	.	T	G,A	0	.	AC=2,2;AF=0.027027027027,0.027027027027	GT:AC:AF:NC	0:2,0:0.0526315789474,0:T=36,G=2,	0:0,2:0,0.0555555555556:A=2,T=34,G=1,
+phiX174	1359	.	T	G	0	.	AC=3;AF=0.0405405405405	GT:AC:AF:NC	0:3:0.0714285714286:T=39,G=3,	0:0:0:T=32,G=1,
+phiX174	1366	.	G	C	0	.	AC=3;AF=0.0428571428571	GT:AC:AF:NC	0:3:0.0714285714286:C=3,T=1,G=39,	0:0:0:G=28,
+phiX174	1370	.	T	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:0:0:C=1,T=45,G=1,	0:2:0.0833333333333:A=1,T=22,G=2,
+phiX174	1373	.	T	G	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0408163265306:T=47,G=2,	0:0:0:T=25,
+phiX174	1380	.	G	C	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:0,2:0,0.0384615384615:C=2,T=1,G=50,	0:0,0:0,0:T=1,G=32,
+phiX174	1381	.	T	G	0	.	AC=3;AF=0.0361445783133	GT:AC:AF:NC	0:3:0.0576923076923:A=1,T=49,G=3,	0:0:0:T=31,
+phiX174	1384	.	T	G	0	.	AC=4;AF=0.0512820512821	GT:AC:AF:NC	0:4:0.0816326530612:T=45,G=4,	0:0:0:T=29,G=1,
+phiX174	1385	.	T	G	0	.	AC=4;AF=0.0512820512821	GT:AC:AF:NC	0:4:0.0816326530612:T=45,G=4,	0:0:0:C=1,T=29,
+phiX174	1391	.	A	C	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:2:0.0408163265306:A=47,C=2,	0:0:0:A=30,C=1,
+phiX174	1393	.	C	A	0	.	AC=2;AF=0.0253164556962	GT:AC:AF:NC	0:2:0.0425531914894:A=2,C=45,	0:0:0:C=32,
+phiX174	1396	.	T	G	0	.	AC=3;AF=0.04	GT:AC:AF:NC	0:3:0.0697674418605:T=40,G=3,	0:0:0:T=32,
+phiX174	1397	.	A	C	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:0:0:A=42,T=1,	0:2:0.0625:A=30,C=2,
+phiX174	1400	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.047619047619:A=40,C=2,G=1,	0:0:0:A=31,
+phiX174	1401	.	A	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.047619047619:A=40,C=2,	0:0:0:A=31,
+phiX174	1434	.	C	T	0	.	AC=2;AF=0.0571428571429	GT:AC:AF:NC	0:2:0.142857142857:C=12,T=2,	0:0:0:C=21,
+phiX174	1438	.	G	C	0	.	AC=2;AF=0.0526315789474	GT:AC:AF:NC	0:0:0:G=15,	0:2:0.0869565217391:C=2,G=21,
+phiX174	1450	.	T	G	0	.	AC=3;AF=0.0789473684211	GT:AC:AF:NC	0:3:0.176470588235:A=1,C=1,T=14,G=3,	0:0:0:T=21,G=1,
+phiX174	1459	.	T	G	0	.	AC=3;AF=0.075	GT:AC:AF:NC	0:3:0.142857142857:C=1,T=18,G=3,	0:0:0:T=19,
+phiX174	1470	.	C	A	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:0:0:C=29,	0:2:0.0869565217391:A=2,C=21,
+phiX174	1484	.	C	G	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:2:0.0740740740741:A=1,C=25,G=2,	0:0:0:C=25,
+phiX174	1487	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0689655172414:T=27,G=2,	0:0:0:T=26,
+phiX174	1491	.	G	T	0	.	AC=2;AF=0.037037037037	GT:AC:AF:NC	0:0:0:T=1,G=26,	0:2:0.0714285714286:T=2,G=26,
+phiX174	1503	.	A	C	0	.	AC=2;AF=0.0454545454545	GT:AC:AF:NC	0:0:0:A=20,C=1,	0:2:0.0833333333333:A=22,C=2,
+phiX174	1536	.	A	C	0	.	AC=3;AF=0.0545454545455	GT:AC:AF:NC	0:3:0.111111111111:A=24,C=3,	0:0:0:A=28,C=1,
+phiX174	1541	.	T	G	0	.	AC=2;AF=0.0363636363636	GT:AC:AF:NC	0:2:0.0740740740741:T=25,G=2,	0:0:0:T=28,
+phiX174	1553	.	A	G	0	.	AC=2;AF=0.0408163265306	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=1,G=2,	0:0:0:A=28,
+phiX174	1566	.	C	G	0	.	AC=2;AF=0.0307692307692	GT:AC:AF:NC	0:0:0:C=30,T=1,	0:2:0.0571428571429:C=33,G=2,
+phiX174	1572	.	T	G	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:2:0.0625:T=30,G=2,	0:0:0:T=32,
+phiX174	1574	.	G	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0882352941176:C=3,G=31,	0:0:0:G=34,
+phiX174	1575	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=33,T=1,
+phiX174	1577	.	A	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:0:0:A=35,	0:3:0.0909090909091:A=30,C=3,
+phiX174	1581	.	T	G	0	.	AC=2;AF=0.0285714285714	GT:AC:AF:NC	0:2:0.0555555555556:C=1,T=34,G=2,	0:0:0:T=34,G=1,
+phiX174	1591	.	A	C	0	.	AC=3;AF=0.037037037037	GT:AC:AF:NC	0:3:0.0789473684211:A=35,C=3,	0:0:0:A=43,C=1,
+phiX174	1597	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0606060606061:T=31,G=2,	0:0:0:C=1,T=45,
+phiX174	1603	.	T	G	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0625:T=30,G=2,	0:0:0:T=50,
+phiX174	1606	.	T	G	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0666666666667:A=1,C=1,T=28,G=2,	0:0:0:T=52,
+phiX174	1611	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0769230769231:A=24,C=2,	0:0:0:A=56,C=1,
+phiX174	1613	.	A	G	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:0:0:A=25,C=1,G=1,	0:2:0.0344827586207:A=56,G=2,
+phiX174	1618	.	C	A	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0666666666667:A=2,C=28,G=1,	0:0:0:A=1,C=57,
+phiX174	1619	.	C	A	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0666666666667:A=2,C=28,	0:0:0:C=57,
+phiX174	1621	.	A	C	0	.	AC=3;AF=0.0352941176471	GT:AC:AF:NC	0:0:0:A=31,T=1,G=1,	0:3:0.0555555555556:A=51,C=3,
+phiX174	1622	.	G	T	0	.	AC=2;AF=0.0224719101124	GT:AC:AF:NC	0:0:0:A=1,G=35,	0:2:0.037037037037:T=2,G=52,
+phiX174	1624	.	A	G	0	.	AC=2;AF=0.0235294117647	GT:AC:AF:NC	0:2:0.0555555555556:A=34,T=1,G=2,	0:0:0:A=49,
+phiX174	1627	.	T	G,C	0	.	AC=2,2;AF=0.0246913580247,0.0246913580247	GT:AC:AF:NC	0:0,0:0,0:T=37,G=1,	0:2,2:0.0454545454545,0.0454545454545:C=2,T=40,G=2,
+phiX174	1630	.	T	G	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:2:0.0526315789474:C=1,T=36,G=2,	0:0:0:T=40,
+phiX174	1632	.	A	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=41,
+phiX174	1634	.	T	G	0	.	AC=4;AF=0.0493827160494	GT:AC:AF:NC	0:2:0.047619047619:C=1,T=40,G=2,	0:2:0.0512820512821:T=37,G=2,
+phiX174	1635	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0714285714286:T=39,G=3,	0:0:0:T=40,
+phiX174	1639	.	G	A	0	.	AC=3;AF=0.0405405405405	GT:AC:AF:NC	0:3:0.075:A=3,T=1,G=37,	0:0:0:G=34,
+phiX174	1645	.	T	G	0	.	AC=2;AF=0.027027027027	GT:AC:AF:NC	0:2:0.0425531914894:T=45,G=2,	0:0:0:T=27,G=1,
+phiX174	1647	.	A	T,G,C	0	.	AC=2,2,2;AF=0.0253164556962,0.0253164556962,0.0253164556962	GT:AC:AF:NC	0:0,2,2:0,0.0384615384615,0.0384615384615:A=48,C=2,G=2,	0:2,0,0:0.0740740740741,0,0:A=25,T=2,
+phiX174	1652	.	G	A	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.037037037037:A=2,G=52,	0:0:0:G=29,
+phiX174	1656	.	T	G	0	.	AC=3;AF=0.0348837209302	GT:AC:AF:NC	0:3:0.0555555555556:T=51,G=3,	0:0:0:T=32,G=1,
+phiX174	1658	.	A	C	0	.	AC=2;AF=0.0240963855422	GT:AC:AF:NC	0:2:0.04:A=48,C=2,	0:0:0:A=33,
+phiX174	1659	.	T	G	0	.	AC=3;AF=0.0365853658537	GT:AC:AF:NC	0:3:0.0625:C=1,T=45,G=3,	0:0:0:T=34,
+phiX174	1663	.	T	G	0	.	AC=4;AF=0.0454545454545	GT:AC:AF:NC	0:0:0:A=1,T=53,	0:4:0.114285714286:T=31,G=4,
+phiX174	1665	.	C	A	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0384615384615:A=2,C=50,G=1,	0:0:0:C=42,
+phiX174	1670	.	G	C	0	.	AC=2;AF=0.0224719101124	GT:AC:AF:NC	0:0,2:0,0.0408163265306:A=1,C=2,T=1,G=47,	0:0,0:0,0:T=1,G=40,
+phiX174	1671	.	G	T	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0384615384615:C=1,T=2,G=50,	0:0:0:G=42,
+phiX174	1672	.	A	G	0	.	AC=3;AF=0.0315789473684	GT:AC:AF:NC	0:3:0.0545454545455:A=52,G=3,	0:0:0:A=40,T=1,
+phiX174	1673	.	G	T	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:2:0.0377358490566:C=1,T=2,G=51,	0:0:0:G=41,
+phiX174	1674	.	G	T	0	.	AC=4;AF=0.04	GT:AC:AF:NC	0:4:0.0714285714286:T=4,G=52,	0:0:0:G=44,
+phiX174	1677	.	A	C	0	.	AC=3;AF=0.031914893617	GT:AC:AF:NC	0:3:0.0566037735849:A=50,C=3,N=1,	0:0:0:A=41,
+phiX174	1678	.	A	C	0	.	AC=2;AF=0.0212765957447	GT:AC:AF:NC	0:0:0:A=54,C=1,G=1,	0:2:0.05:A=38,C=2,
+phiX174	1682	.	T	C	0	.	AC=2;AF=0.0229885057471	GT:AC:AF:NC	0:2:0.0408163265306:C=2,T=47,G=1,	0:0:0:C=1,T=38,
+phiX174	1685	.	T	G	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:2:0.046511627907:A=1,T=41,G=2,	0:0:0:T=41,G=1,
+phiX174	1686	.	A	C	0	.	AC=2;AF=0.025641025641	GT:AC:AF:NC	0:0:0:A=39,C=1,T=1,G=1,	0:2:0.0512820512821:A=37,C=2,
+phiX174	1687	.	T	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.047619047619:C=2,T=40,	0:0:0:A=1,T=40,
+phiX174	1688	.	G	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.05:C=2,T=1,G=38,	0:0:0:G=41,
+phiX174	1689	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0487804878049:A=39,C=2,T=1,G=1,	0:0:0:A=41,C=1,
+phiX174	1691	.	G	C	0	.	AC=2;AF=0.025974025974	GT:AC:AF:NC	0:2:0.05:C=2,T=1,G=38,	0:0:0:G=37,
+phiX174	1693	.	T	G	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:0:0:T=42,G=1,	0:2:0.047619047619:T=40,G=2,
+phiX174	1695	.	A	C	0	.	AC=2;AF=0.0232558139535	GT:AC:AF:NC	0:2:0.046511627907:A=41,C=2,	0:0:0:A=43,C=1,
+phiX174	1702	.	T	C	0	.	AC=2;AF=0.0246913580247	GT:AC:AF:NC	0:2:0.0512820512821:A=1,C=2,T=37,	0:0:0:C=1,T=42,
+phiX174	1707	.	T	C	0	.	AC=2;AF=0.0232558139535	GT:AC:AF:NC	0:2:0.05:C=2,T=38,	0:0:0:T=46,G=1,
+phiX174	1708	.	A	C	0	.	AC=2;AF=0.0238095238095	GT:AC:AF:NC	0:2:0.0526315789474:A=36,C=2,	0:0:0:A=46,C=1,G=1,
+phiX174	1710	.	T	C	0	.	AC=2;AF=0.025	GT:AC:AF:NC	0:0:0:C=1,T=34,	0:2:0.0434782608696:C=2,T=44,
+phiX174	1723	.	T	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0588235294118:C=2,T=32,	0:0:0:T=48,
+phiX174	1724	.	A	C	0	.	AC=4;AF=0.0481927710843	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:2:0.0416666666667:A=46,C=2,
+phiX174	1725	.	A	C	0	.	AC=2;AF=0.0243902439024	GT:AC:AF:NC	0:2:0.0606060606061:A=31,C=2,	0:0:0:A=49,
+phiX174	1726	.	T	G	0	.	AC=3;AF=0.037037037037	GT:AC:AF:NC	0:3:0.0882352941176:T=31,G=3,	0:0:0:C=1,T=47,
+phiX174	1730	.	T	G	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:0:0:C=1,T=28,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1739	.	G	T	0	.	AC=3;AF=0.0434782608696	GT:AC:AF:NC	0:3:0.09375:T=3,G=29,	0:0:0:G=37,
+phiX174	1746	.	A	C	0	.	AC=2;AF=0.0338983050847	GT:AC:AF:NC	0:2:0.0689655172414:A=27,C=2,	0:0:0:A=30,C=1,G=1,
+phiX174	1749	.	T	G,C	0	.	AC=3,2;AF=0.044776119403,0.0298507462687	GT:AC:AF:NC	0:3,2:0.0857142857143,0.0571428571429:C=2,T=30,G=3,	0:0,0:0,0:T=32,G=1,
+phiX174	1755	.	G	C	0	.	AC=2;AF=0.027397260274	GT:AC:AF:NC	0:2:0.05:C=2,G=38,	0:0:0:C=1,G=33,
+phiX174	1756	.	A	G,C	0	.	AC=2,2;AF=0.0281690140845,0.0281690140845	GT:AC:AF:NC	0:0,2:0,0.0526315789474:A=36,C=2,T=1,G=1,	0:2,0:0.0606060606061,0:A=31,C=1,G=2,
+phiX174	1761	.	A	G	0	.	AC=2;AF=0.0285714285714	GT:AC:AF:NC	0:2:0.0512820512821:A=37,C=1,G=2,	0:0:0:A=31,
+phiX174	1764	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.05:A=38,C=2,	0:0:0:A=28,
+phiX174	1766	.	A	C	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0487804878049:A=39,C=2,	0:0:0:A=27,
+phiX174	1769	.	T	G	0	.	AC=2;AF=0.03125	GT:AC:AF:NC	0:2:0.0526315789474:A=1,C=1,T=36,G=2,	0:0:0:T=26,G=1,
+phiX174	1771	.	G	C	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:2:0.0526315789474:C=2,G=36,	0:0:0:G=29,
+phiX174	1772	.	T	G	0	.	AC=4;AF=0.0597014925373	GT:AC:AF:NC	0:4:0.108108108108:C=1,T=33,G=4,	0:0:0:T=30,
+phiX174	1774	.	A	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0810810810811:A=34,G=3,	0:0:0:A=31,
+phiX174	1775	.	G	A	0	.	AC=2;AF=0.030303030303	GT:AC:AF:NC	0:2:0.0571428571429:A=2,G=33,	0:0:0:G=31,
+phiX174	1776	.	G	T	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0540540540541:T=2,G=35,	0:0:0:G=32,
+phiX174	1778	.	C	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0526315789474:C=36,G=2,	0:0:0:C=31,
+phiX174	1779	.	A	C	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.075:A=37,C=3,	0:0:0:A=28,
+phiX174	1781	.	T	G	0	.	AC=5;AF=0.0714285714286	GT:AC:AF:NC	0:2:0.0487804878049:C=1,T=39,G=2,	0:3:0.103448275862:T=26,G=3,
+phiX174	1782	.	T	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.075:T=37,G=3,	0:0:0:A=1,T=28,
+phiX174	1783	.	T	C	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:2:0.0512820512821:C=2,T=37,	0:0:0:T=28,G=1,
+phiX174	1788	.	G	T	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:0:0:A=1,T=1,G=29,	0:2:0.0689655172414:T=2,G=27,
+phiX174	1792	.	T	G	0	.	AC=2;AF=0.0344827586207	GT:AC:AF:NC	0:2:0.0606060606061:T=31,G=2,	0:0:0:T=25,G=1,
+phiX174	1802	.	A	C	0	.	AC=2;AF=0.030303030303	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=31,C=1,
+phiX174	1804	.	C	T	0	.	AC=2;AF=0.0294117647059	GT:AC:AF:NC	0:2:0.0588235294118:A=1,C=32,T=2,	0:0:0:C=34,
+phiX174	1805	.	T	G	0	.	AC=3;AF=0.0441176470588	GT:AC:AF:NC	0:3:0.0857142857143:T=32,G=3,	0:0:0:T=33,G=1,
+phiX174	1806	.	A	C	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:2:0.0571428571429:A=33,C=2,	0:0:0:A=34,G=1,
+phiX174	1824	.	G	C	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0714285714286:C=2,T=1,G=26,	0:0:0:G=43,
+phiX174	1825	.	T	G	0	.	AC=2;AF=0.0281690140845	GT:AC:AF:NC	0:2:0.0689655172414:T=27,G=2,	0:0:0:T=42,G=1,
+phiX174	1833	.	T	G	0	.	AC=2;AF=0.0289855072464	GT:AC:AF:NC	0:0:0:A=1,T=24,	0:2:0.0444444444444:T=43,G=2,
+phiX174	1843	.	T	G	0	.	AC=2;AF=0.031746031746	GT:AC:AF:NC	0:2:0.08:A=1,T=23,G=2,	0:0:0:T=38,
+phiX174	1847	.	A	C	0	.	AC=2;AF=0.0333333333333	GT:AC:AF:NC	0:2:0.0833333333333:A=22,C=2,T=1,G=1,	0:0:0:A=36,
+phiX174	1854	.	T	G	0	.	AC=2;AF=0.0298507462687	GT:AC:AF:NC	0:0:0:T=26,	0:2:0.0487804878049:T=39,G=2,
+phiX174	1860	.	T	G	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:0:0:A=1,T=20,	0:2:0.0606060606061:T=31,G=2,
+phiX174	1863	.	C	G	0	.	AC=2;AF=0.0384615384615	GT:AC:AF:NC	0:2:0.105263157895:A=1,C=17,G=2,	0:0:0:C=33,
+phiX174	1866	.	T	G	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:0:0:T=19,G=1,	0:2:0.0645161290323:T=29,G=2,
+phiX174	1875	.	T	A	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:2:0.0952380952381:A=2,T=19,	0:0:0:T=32,G=1,
+phiX174	1876	.	T	G	0	.	AC=3;AF=0.0588235294118	GT:AC:AF:NC	0:3:0.157894736842:C=1,T=16,G=3,	0:0:0:T=32,G=1,
+phiX174	1882	.	T	G	0	.	AC=2;AF=0.0377358490566	GT:AC:AF:NC	0:2:0.105263157895:T=17,G=2,	0:0:0:T=34,
+phiX174	1889	.	A	C	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:2:0.0869565217391:A=21,C=2,	0:0:0:A=27,
+phiX174	1913	.	G	C	0	.	AC=2;AF=0.04	GT:AC:AF:NC	0:0:0:G=27,	0:2:0.0869565217391:C=2,G=21,
+phiX174	1915	.	T	G	0	.	AC=2;AF=0.0425531914894	GT:AC:AF:NC	0:0:0:A=1,T=24,	0:2:0.0869565217391:T=21,G=2,
+phiX174	1917	.	A	T	0	.	AC=2;AF=0.0444444444444	GT:AC:AF:NC	0:2,0:0.0833333333333,0:A=22,T=2,G=1,	0:0,0:0,0:A=21,G=1,
+phiX174	1930	.	T	A	0	.	AC=2;AF=0.0526315789474	GT:AC:AF:NC	0:2:0.0909090909091:A=2,T=20,	0:0:0:T=16,
+phiX174	1934	.	A	C	0	.	AC=2;AF=0.0512820512821	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=2,T=1,	0:0:0:A=18,C=1,
+phiX174	1940	.	A	C	0	.	AC=2;AF=0.046511627907	GT:AC:AF:NC	0:2:0.0952380952381:A=19,C=2,	0:0:0:A=22,C=1,
+phiX174	1942	.	T	G	0	.	AC=2;AF=0.047619047619	GT:AC:AF:NC	0:0:0:T=19,	0:2:0.0869565217391:T=21,G=2,
+phiX174	1945	.	T	G	0	.	AC=3;AF=0.0769230769231	GT:AC:AF:NC	0:0:0:T=15,G=1,	0:3:0.125:T=21,G=3,
+phiX174	1954	.	T	C	0	.	AC=2;AF=0.0625	GT:AC:AF:NC	0:0,0:0,0:T=12,G=1,	0:0,2:0,0.1:C=2,T=18,G=1,
+phiX174	1959	.	T	G	0	.	AC=2;AF=0.0555555555556	GT:AC:AF:NC	0:2:0.153846153846:T=11,G=2,	0:0:0:T=23,
+phiX174	1994	.	A	C	0	.	AC=2;AF=0.0512820512821	GT:AC:AF:NC	0:2:0.153846153846:A=11,C=2,	0:0:0:A=26,
+phiX174	1998	.	A	G	0	.	AC=2;AF=0.05	GT:AC:AF:NC	0:2:0.142857142857:A=12,G=2,	0:0:0:A=26,
diff --git a/tool_collections/vcflib/vcfvcfintersect/tool-data/fasta_indexes.loc.sample b/tool_collections/vcflib/vcfvcfintersect/tool-data/fasta_indexes.loc.sample
new file mode 120000
index 00000000000..6d7aec6c449
--- /dev/null
+++ b/tool_collections/vcflib/vcfvcfintersect/tool-data/fasta_indexes.loc.sample
@@ -0,0 +1 @@
+../../tool-data/fasta_indexes.loc.sample
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfvcfintersect/tool-data/tool_data_table_conf.xml.sample b/tool_collections/vcflib/vcfvcfintersect/tool-data/tool_data_table_conf.xml.sample
new file mode 120000
index 00000000000..7bfcf015ca6
--- /dev/null
+++ b/tool_collections/vcflib/vcfvcfintersect/tool-data/tool_data_table_conf.xml.sample
@@ -0,0 +1 @@
+../../tool-data/tool_data_table_conf.xml.sample
\ No newline at end of file
diff --git a/tool_collections/vcflib/vcfvcfintersect/vcfvcfintersect.xml b/tool_collections/vcflib/vcfvcfintersect/vcfvcfintersect.xml
new file mode 100644
index 00000000000..129e5a5ab23
--- /dev/null
+++ b/tool_collections/vcflib/vcfvcfintersect/vcfvcfintersect.xml
@@ -0,0 +1,128 @@
+<tool id="vcfvcfintersect" name="VCF-VCFintersect:" version="@WRAPPER_VERSION@.0">
+    <description>Intersect two VCF datasets</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"/>
+    <expand macro="stdio" />
+    <command>
+        #set $reference_fasta_filename = "localref.fa"
+        #if str( $reference_source.reference_source_selector ) == "history":
+           ln -s "${reference_source.ref_file}" "${reference_fasta_filename}" &amp;&amp;
+        #else:
+           #set $reference_fasta_filename = str( $reference_source.ref_file.fields.path )
+        #end if
+
+        #if $adv_options.adv_options_selector == "use_adv_controls":
+            vcfintersect "${adv_options.adv_options_input}" ${loci} ${invert} -r "${reference_fasta_filename}" -w "${window_size}" ${isect_union} "${vcf_input1}" "${vcf_input2}" > "${out_file1}"
+        #else:
+            vcfintersect ${loci} ${invert} -r "${reference_fasta_filename}" -w "${window_size}" ${isect_union} "${vcf_input1}" "${vcf_input2}" > "${out_file1}"
+        #end if
+    </command>
+    <inputs>
+        <!-- selecting refernce source -->
+        <param name="vcf_input1" type="data" format="vcf" label="The first VCF dataset" />
+        <param name="vcf_input2" type="data" format="vcf" label="The second VCF dataset"
+            help="The second dataset will be instersected with the first"/>
+        <conditional name="reference_source">
+            <param name="reference_source_selector" type="select" label="Choose the source for the reference genome"
+                help="This tools needs to access reference genomes sequence specified by this option.">
+                <option value="cached">Locally cached</option>
+                <option value="history">History</option>
+            </param>
+            <when value="cached">
+                <param name="ref_file" type="select" label="Select reference genome">
+                    <options from_data_table="fasta_indexes">
+                    <!--<filter type="data_meta" key="dbkey" ref="input_bam" column="value"/>-->
+                    </options>
+                    <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/>
+                </param>
+            </when>
+            <when value="history"> <!-- FIX ME!!!! -->
+                <param name="ref_file" type="data" format="fasta" label="Using reference file" />
+            </when>
+        </conditional>
+        <param name="isect_union" type="select" label="Union or intersection" help="-i or -u">
+            <option value="-i">Intersect</option>
+            <option value="-u">Union</option>
+        </param>
+        <param name="invert" type="boolean" truevalue="-v" falsevalue="" label="Invert selection?" help="-v, --invert" />
+        <param name="window_size" type="integer" value="30"
+            label="compare records up to this many bp away (window size)" help="-w, --window-size" />
+        <param name="loci" type="boolean" truevalue="-l" falsevalue=""
+            label="output whole loci when one alternate allele matches" help="-l, --loci" />
+        <conditional name="adv_options">
+            <param name="adv_options_selector" type="select" label="Advanced controls" help="Allows you to specify options that are not listed above">
+                <option value="no">Don't use advanced options</option>
+                <option value="use_adv_controls">Use advanced options</option>
+            </param>
+            <when value="no" />
+            <when value="use_adv_controls">
+                <param name="adv_options_input" type="text" value="-t &quot;vcfvcf-intersect-result&quot;"
+                    label="Enter additional command line options described in the help section below"
+                    help="such as -m, -t, -V, -M, and -T">
+                    <sanitizer>
+                        <valid initial="string.printable">
+                            <remove value="&apos;"/>
+                        </valid>
+                        <mapping initial="none">
+                            <add source="&apos;" target="__sq__"/>
+                        </mapping>
+                    </sanitizer>
+                </param>
+            </when>
+        </conditional>
+    </inputs>
+    <outputs>
+        <data format="vcf" name="out_file1" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="vcf_input1" value="vcfvcfintersect-input1.vcf" />
+            <param name="vcf_input2" value="vcfvcfintersect-input2.vcf" />
+            <param name="reference_source_selector" value="history" />
+            <param name="ref_file" value="vcflib-test-genome-phix.fa" />
+            <param name="isect_union" value="-i" />
+            <param name="invert" value="False" />
+            <param name="loci" value="False" />
+            <param name="adv_options_selector" value="no" />
+            <param name="window_size" value="30" />
+            <output name="out_file1" file="vcfvcfintersect-test1.vcf"/>
+        </test>
+        <test>
+            <param name="reference_source_selector" value="history" />
+            <param name="vcf_input1" value="vcfvcfintersect-input1.vcf" />
+            <param name="vcf_input2" value="vcfvcfintersect-input2.vcf" />
+            <param name="isect_union" value="-u" />
+            <param name="invert" value="False" />
+            <param name="loci" value="False" />
+            <param name="adv_options_selector" value="no" />
+            <param name="window_size" value="30" />
+            <param name="ref_file" value="vcflib-test-genome-phix.fa" />
+            <output name="out_file1" file="vcfvcfintersect-test2.vcf"/>
+        </test>
+    </tests>
+    <help>
+Computes intersections and unions for two VCF datasets. Unifies equivalent alleles within window-size bp.
+
+The options are::
+
+    -v, --invert              invert the selection, printing only records which would
+    -i, --intersect-vcf FILE  use this VCF for set intersection generation
+    -u, --union-vcf FILE      use this VCF for set union generation
+    -w, --window-size N       compare records up to this many bp away (default 30)
+    -l, --loci                output whole loci when one alternate allele matches
+    -m, --ref-match           intersect on the basis of record REF string
+    -t, --tag TAG             attach TAG to each record's info field if it would intersect
+    -V, --tag-value VAL       use this value to indicate that the allele is passing
+                              '.' will be used otherwise.  default: 'PASS'
+    -M, --merge-from FROM-TAG
+    -T, --merge-to   TO-TAG   merge from FROM-TAG used in the -i file, setting TO-TAG
+                              in the current file.
+
+----
+
+VCFVCFintersect is based on vcfintersect utility of VCFlib toolkit developed by Erik Garrison (https://github.com/ekg/vcflib).
+    </help>
+    <expand macro="citations" />
+</tool>

From a0eb535f6820dc400faa67dcd9b1762ca0e80059 Mon Sep 17 00:00:00 2001
From: Martin Cech <cech.marten@gmail.com>
Date: Tue, 24 Jan 2017 11:44:52 -0500
Subject: [PATCH 02/40] change shed.yml: remote repo url to iuc

---
 tool_collections/vcflib/vcf2tsv/.shed.yml              | 2 +-
 tool_collections/vcflib/vcfaddinfo/.shed.yml           | 2 +-
 tool_collections/vcflib/vcfallelicprimitives/.shed.yml | 2 +-
 tool_collections/vcflib/vcfannotate/.shed.yml          | 2 +-
 tool_collections/vcflib/vcfannotategenotypes/.shed.yml | 2 +-
 tool_collections/vcflib/vcfbedintersect/.shed.yml      | 2 +-
 tool_collections/vcflib/vcfbreakcreatemulti/.shed.yml  | 2 +-
 tool_collections/vcflib/vcfcheck/.shed.yml             | 2 +-
 tool_collections/vcflib/vcfcombine/.shed.yml           | 2 +-
 tool_collections/vcflib/vcfcommonsamples/.shed.yml     | 2 +-
 tool_collections/vcflib/vcfdistance/.shed.yml          | 2 +-
 tool_collections/vcflib/vcffilter/.shed.yml            | 2 +-
 tool_collections/vcflib/vcffixup/.shed.yml             | 2 +-
 tool_collections/vcflib/vcfflatten/.shed.yml           | 2 +-
 tool_collections/vcflib/vcfgeno2haplo/.shed.yml        | 2 +-
 tool_collections/vcflib/vcfgenotypes/.shed.yml         | 2 +-
 tool_collections/vcflib/vcfhethom/.shed.yml            | 2 +-
 tool_collections/vcflib/vcfleftalign/.shed.yml         | 2 +-
 tool_collections/vcflib/vcfprimers/.shed.yml           | 2 +-
 tool_collections/vcflib/vcfrandomsample/.shed.yml      | 2 +-
 tool_collections/vcflib/vcfselectsamples/.shed.yml     | 2 +-
 tool_collections/vcflib/vcfsort/.shed.yml              | 2 +-
 tool_collections/vcflib/vcfvcfintersect/.shed.yml      | 2 +-
 23 files changed, 23 insertions(+), 23 deletions(-)

diff --git a/tool_collections/vcflib/vcf2tsv/.shed.yml b/tool_collections/vcflib/vcf2tsv/.shed.yml
index 5cc2e89eb48..46766cec405 100644
--- a/tool_collections/vcflib/vcf2tsv/.shed.yml
+++ b/tool_collections/vcflib/vcf2tsv/.shed.yml
@@ -10,5 +10,5 @@ long_description: |
   by Erik Garrison (https://github.com/ekg/vcflib).
 name: vcf2tsv
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcf2tsv
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcf2tsv
 type: unrestricted
diff --git a/tool_collections/vcflib/vcfaddinfo/.shed.yml b/tool_collections/vcflib/vcfaddinfo/.shed.yml
index fd27f3c964c..1e25fd2a5c8 100644
--- a/tool_collections/vcflib/vcfaddinfo/.shed.yml
+++ b/tool_collections/vcflib/vcfaddinfo/.shed.yml
@@ -7,5 +7,5 @@ long_description: |
   Adds info fields from the second dataset which are not present in the first dataset.
 name: vcfaddinfo
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfaddinfo
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcfaddinfo
 type: unrestricted
diff --git a/tool_collections/vcflib/vcfallelicprimitives/.shed.yml b/tool_collections/vcflib/vcfallelicprimitives/.shed.yml
index 22105f331c2..0d6ae31de22 100644
--- a/tool_collections/vcflib/vcfallelicprimitives/.shed.yml
+++ b/tool_collections/vcflib/vcfallelicprimitives/.shed.yml
@@ -10,5 +10,5 @@ long_description: |
   genotypes in the input.
 name: vcfallelicprimitives
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfallelicprimitives
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcfallelicprimitives
 type: unrestricted
diff --git a/tool_collections/vcflib/vcfannotate/.shed.yml b/tool_collections/vcflib/vcfannotate/.shed.yml
index a5339a1d867..40c3537e71b 100644
--- a/tool_collections/vcflib/vcfannotate/.shed.yml
+++ b/tool_collections/vcflib/vcfannotate/.shed.yml
@@ -7,5 +7,5 @@ long_description: |
   a BED file. Intersections are done on the reference sequences in the VCF file.
 name: vcfannotate
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfannotate
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcfannotate
 type: unrestricted
diff --git a/tool_collections/vcflib/vcfannotategenotypes/.shed.yml b/tool_collections/vcflib/vcfannotategenotypes/.shed.yml
index 8853abfb1b5..77ef90bd9d0 100644
--- a/tool_collections/vcflib/vcfannotategenotypes/.shed.yml
+++ b/tool_collections/vcflib/vcfannotategenotypes/.shed.yml
@@ -9,5 +9,5 @@ long_description: |
   a ''has\_variant'' flag for sites where the second file has a variant.
 name: vcfannotategenotypes
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfannotategenotypes
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcfannotategenotypes
 type: unrestricted
diff --git a/tool_collections/vcflib/vcfbedintersect/.shed.yml b/tool_collections/vcflib/vcfbedintersect/.shed.yml
index fe813c93942..949f49cb03b 100644
--- a/tool_collections/vcflib/vcfbedintersect/.shed.yml
+++ b/tool_collections/vcflib/vcfbedintersect/.shed.yml
@@ -8,5 +8,5 @@ long_description: |
   or a manually typed interval (in the form of chr:start-end).
 name: vcfbedintersect
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfbedintersect
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcfbedintersect
 type: unrestricted
diff --git a/tool_collections/vcflib/vcfbreakcreatemulti/.shed.yml b/tool_collections/vcflib/vcfbreakcreatemulti/.shed.yml
index 4f46813a267..7ff96c09291 100644
--- a/tool_collections/vcflib/vcfbreakcreatemulti/.shed.yml
+++ b/tool_collections/vcflib/vcfbreakcreatemulti/.shed.yml
@@ -13,5 +13,5 @@ long_description: |
   them into a single record.
 name: vcfbreakcreatemulti
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfbreakcreatemulti
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcfbreakcreatemulti
 type: unrestricted
diff --git a/tool_collections/vcflib/vcfcheck/.shed.yml b/tool_collections/vcflib/vcfcheck/.shed.yml
index 32686dc3ed5..efe75c86012 100644
--- a/tool_collections/vcflib/vcfcheck/.shed.yml
+++ b/tool_collections/vcflib/vcfcheck/.shed.yml
@@ -6,5 +6,5 @@ long_description: |
   Verifies that the VCF REF field matches the reference as described
 name: vcfcheck
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfcheck
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcfcheck
 type: unrestricted
diff --git a/tool_collections/vcflib/vcfcombine/.shed.yml b/tool_collections/vcflib/vcfcombine/.shed.yml
index f7b169b5f25..e7996d61b67 100644
--- a/tool_collections/vcflib/vcfcombine/.shed.yml
+++ b/tool_collections/vcflib/vcfcombine/.shed.yml
@@ -10,5 +10,5 @@ long_description: |
   record. If they do not, multiple records will be emitted.
 name: vcfcombine
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfcombine
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcfcombine
 type: unrestricted
diff --git a/tool_collections/vcflib/vcfcommonsamples/.shed.yml b/tool_collections/vcflib/vcfcommonsamples/.shed.yml
index f00bce1692c..684327a8031 100644
--- a/tool_collections/vcflib/vcfcommonsamples/.shed.yml
+++ b/tool_collections/vcflib/vcfcommonsamples/.shed.yml
@@ -6,5 +6,5 @@ long_description: |
   Outputs each record in the first file, removing samples not present in the second.
 name: vcfcommonsamples
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfcommonsamples
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcfcommonsamples
 type: unrestricted
diff --git a/tool_collections/vcflib/vcfdistance/.shed.yml b/tool_collections/vcflib/vcfdistance/.shed.yml
index 533bc22d874..26cc59189ea 100644
--- a/tool_collections/vcflib/vcfdistance/.shed.yml
+++ b/tool_collections/vcflib/vcfdistance/.shed.yml
@@ -10,5 +10,5 @@ long_description: |
   in ascending order).
 name: vcfdistance
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfdistance
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcfdistance
 type: unrestricted
diff --git a/tool_collections/vcflib/vcffilter/.shed.yml b/tool_collections/vcflib/vcffilter/.shed.yml
index 07e608f54ea..48519581713 100644
--- a/tool_collections/vcflib/vcffilter/.shed.yml
+++ b/tool_collections/vcflib/vcffilter/.shed.yml
@@ -7,5 +7,5 @@ long_description: |
   variety of tags. This is a galaxy wrapper for vcffilter utility from vcflib package.
 name: vcffilter
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcffilter
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcffilter
 type: unrestricted
diff --git a/tool_collections/vcflib/vcffixup/.shed.yml b/tool_collections/vcflib/vcffixup/.shed.yml
index 85de4398f40..97c7932d4a5 100644
--- a/tool_collections/vcflib/vcffixup/.shed.yml
+++ b/tool_collections/vcflib/vcffixup/.shed.yml
@@ -7,5 +7,5 @@ long_description: |
   count), AF (alternate allele frequency), NS (number of called), in the VCF records.
 name: vcffixup
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcffixup
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcffixup
 type: unrestricted
diff --git a/tool_collections/vcflib/vcfflatten/.shed.yml b/tool_collections/vcflib/vcfflatten/.shed.yml
index 7f9e1128291..07c679e69f2 100644
--- a/tool_collections/vcflib/vcfflatten/.shed.yml
+++ b/tool_collections/vcflib/vcfflatten/.shed.yml
@@ -8,5 +8,5 @@ long_description: |
   the fields which vary according to the Allele or Genotype.
 name: vcfflatten
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfflatten
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcfflatten
 type: unrestricted
diff --git a/tool_collections/vcflib/vcfgeno2haplo/.shed.yml b/tool_collections/vcflib/vcfgeno2haplo/.shed.yml
index f996dd20e7a..761ec15e4d3 100644
--- a/tool_collections/vcflib/vcfgeno2haplo/.shed.yml
+++ b/tool_collections/vcflib/vcfgeno2haplo/.shed.yml
@@ -8,5 +8,5 @@ long_description: |
   non-phased genotypes on input.
 name: vcfgeno2haplo
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfgeno2haplo
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcfgeno2haplo
 type: unrestricted
diff --git a/tool_collections/vcflib/vcfgenotypes/.shed.yml b/tool_collections/vcflib/vcfgenotypes/.shed.yml
index 58c1a1b3d2e..4125d76b784 100644
--- a/tool_collections/vcflib/vcfgenotypes/.shed.yml
+++ b/tool_collections/vcflib/vcfgenotypes/.shed.yml
@@ -7,5 +7,5 @@ long_description: |
   field) to the alleles provided in the call''s ALT/REF fields.
 name: vcfgenotypes
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfgenotypes
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcfgenotypes
 type: unrestricted
diff --git a/tool_collections/vcflib/vcfhethom/.shed.yml b/tool_collections/vcflib/vcfhethom/.shed.yml
index 6980ba57fa6..0ff2bc3f4be 100644
--- a/tool_collections/vcflib/vcfhethom/.shed.yml
+++ b/tool_collections/vcflib/vcfhethom/.shed.yml
@@ -10,5 +10,5 @@ long_description: |
   (3) Computes the total number of alleles in the input dataset
 name: vcfhethom
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfhethom
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcfhethom
 type: unrestricted
diff --git a/tool_collections/vcflib/vcfleftalign/.shed.yml b/tool_collections/vcflib/vcfleftalign/.shed.yml
index 497c1134cec..6f9db4f6775 100644
--- a/tool_collections/vcflib/vcfleftalign/.shed.yml
+++ b/tool_collections/vcflib/vcfleftalign/.shed.yml
@@ -8,5 +8,5 @@ long_description: |
   have entropy > 1 bit/bp, or be shorter than ~5kb.
 name: vcfleftalign
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfleftalign
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcfleftalign
 type: unrestricted
diff --git a/tool_collections/vcflib/vcfprimers/.shed.yml b/tool_collections/vcflib/vcfprimers/.shed.yml
index 4e273fc06a7..1a00c2f626e 100644
--- a/tool_collections/vcflib/vcfprimers/.shed.yml
+++ b/tool_collections/vcflib/vcfprimers/.shed.yml
@@ -8,5 +8,5 @@ long_description: |
   experiments.  Primers extracted which would flank all of the alleles at multi-allelic sites.
 name: vcfprimers
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfprimers
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcfprimers
 type: unrestricted
diff --git a/tool_collections/vcflib/vcfrandomsample/.shed.yml b/tool_collections/vcflib/vcfrandomsample/.shed.yml
index 608bf958db6..a7d029682ec 100644
--- a/tool_collections/vcflib/vcfrandomsample/.shed.yml
+++ b/tool_collections/vcflib/vcfrandomsample/.shed.yml
@@ -8,5 +8,5 @@ long_description: |
   used to provide uniform sampling across allele frequencies, for instance (AF field in this case).
 name: vcfrandomsample
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfrandomsample
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcfrandomsample
 type: unrestricted
diff --git a/tool_collections/vcflib/vcfselectsamples/.shed.yml b/tool_collections/vcflib/vcfselectsamples/.shed.yml
index c55d36bcbc0..5f3f6197a41 100644
--- a/tool_collections/vcflib/vcfselectsamples/.shed.yml
+++ b/tool_collections/vcflib/vcfselectsamples/.shed.yml
@@ -7,5 +7,5 @@ long_description: |
   vcfkeepsamples and vcfremovesamples from VCFlib package into a single utility.
 name: vcfselectsamples
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfselectsamples
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcfselectsamples
 type: unrestricted
diff --git a/tool_collections/vcflib/vcfsort/.shed.yml b/tool_collections/vcflib/vcfsort/.shed.yml
index 414bea53b29..dc5ac2efe83 100644
--- a/tool_collections/vcflib/vcfsort/.shed.yml
+++ b/tool_collections/vcflib/vcfsort/.shed.yml
@@ -6,5 +6,5 @@ long_description: |
 name: vcfsort
 owner: devteam
 homepage_url: https://github.com/ekg/vcflib
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfsort
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcfsort
 type: unrestricted
diff --git a/tool_collections/vcflib/vcfvcfintersect/.shed.yml b/tool_collections/vcflib/vcfvcfintersect/.shed.yml
index 65835fb4317..04e1c655f4e 100644
--- a/tool_collections/vcflib/vcfvcfintersect/.shed.yml
+++ b/tool_collections/vcflib/vcfvcfintersect/.shed.yml
@@ -7,5 +7,5 @@ long_description: |
   equivalent alleles within window-size bp.
 name: vcfvcfintersect
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/vcflib/vcfvcfintersect
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/vcflib/vcfvcfintersect
 type: unrestricted

From d9c1bf77c87105945ce8482c11f88339420deeab Mon Sep 17 00:00:00 2001
From: Martin Cech <cech.marten@gmail.com>
Date: Tue, 24 Jan 2017 12:58:17 -0500
Subject: [PATCH 03/40] update the rest of the remote repo urls

---
 tool_collections/kraken/kraken/.shed.yml               | 2 +-
 tool_collections/samtools/bam_to_cram/.shed.yml        | 2 +-
 tool_collections/samtools/bam_to_sam/.shed.yml         | 2 +-
 tool_collections/samtools/cram_to_bam/.shed.yml        | 2 +-
 tool_collections/samtools/sam_to_bam/.shed.yml         | 2 +-
 tool_collections/samtools/samtools_bedcov/.shed.yml    | 2 +-
 tool_collections/samtools/samtools_calmd/.shed.yml     | 2 +-
 tool_collections/samtools/samtools_flagstat/.shed.yml  | 2 +-
 tool_collections/samtools/samtools_idxstats/.shed.yml  | 2 +-
 tool_collections/samtools/samtools_mpileup/.shed.yml   | 2 +-
 tool_collections/samtools/samtools_phase/.shed.yml     | 2 +-
 tool_collections/samtools/samtools_reheader/.shed.yml  | 2 +-
 tool_collections/samtools/samtools_rmdup/.shed.yml     | 2 +-
 tool_collections/samtools/samtools_slice_bam/.shed.yml | 2 +-
 tool_collections/samtools/samtools_sort/.shed.yml      | 2 +-
 tool_collections/samtools/samtools_split/.shed.yml     | 2 +-
 tool_collections/samtools/samtools_stats/.shed.yml     | 2 +-
 17 files changed, 17 insertions(+), 17 deletions(-)

diff --git a/tool_collections/kraken/kraken/.shed.yml b/tool_collections/kraken/kraken/.shed.yml
index 609a78b7e9b..00699d0b194 100644
--- a/tool_collections/kraken/kraken/.shed.yml
+++ b/tool_collections/kraken/kraken/.shed.yml
@@ -12,5 +12,5 @@ long_description: |
   and a novel classification algorithm.
 name: kraken
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/blob/master/tool_collections/kraken/kraken/
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/blob/master/tool_collections/kraken/kraken/
 type: unrestricted
diff --git a/tool_collections/samtools/bam_to_cram/.shed.yml b/tool_collections/samtools/bam_to_cram/.shed.yml
index ebd687fbc8e..ba9dcb08cbb 100644
--- a/tool_collections/samtools/bam_to_cram/.shed.yml
+++ b/tool_collections/samtools/bam_to_cram/.shed.yml
@@ -9,5 +9,5 @@ long_description: |
 name: samtools_bam_to_cram
 owner: devteam
 homepage_url: https://samtools.github.io/
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/bam_to_cram
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/bam_to_cram
 type: unrestricted
diff --git a/tool_collections/samtools/bam_to_sam/.shed.yml b/tool_collections/samtools/bam_to_sam/.shed.yml
index cf0ac3af3a0..eb02c933c23 100644
--- a/tool_collections/samtools/bam_to_sam/.shed.yml
+++ b/tool_collections/samtools/bam_to_sam/.shed.yml
@@ -7,5 +7,5 @@ long_description: |
 name: bam_to_sam
 owner: devteam
 homepage_url: http://www.htslib.org/
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/bam_to_sam
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/bam_to_sam
 type: unrestricted
diff --git a/tool_collections/samtools/cram_to_bam/.shed.yml b/tool_collections/samtools/cram_to_bam/.shed.yml
index a6c37c19b63..9b6fa0293e3 100644
--- a/tool_collections/samtools/cram_to_bam/.shed.yml
+++ b/tool_collections/samtools/cram_to_bam/.shed.yml
@@ -7,5 +7,5 @@ long_description: |
 name: samtools_cram_to_bam
 owner: devteam
 homepage_url: https://samtools.github.io/
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/cram_to_bam
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/cram_to_bam
 type: unrestricted
diff --git a/tool_collections/samtools/sam_to_bam/.shed.yml b/tool_collections/samtools/sam_to_bam/.shed.yml
index 6f0370f3b5f..bf2c6168d02 100644
--- a/tool_collections/samtools/sam_to_bam/.shed.yml
+++ b/tool_collections/samtools/sam_to_bam/.shed.yml
@@ -7,5 +7,5 @@ long_description: |
 name: sam_to_bam
 owner: devteam
 homepage_url: http://www.htslib.org/
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/sam_to_bam
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/sam_to_bam
 type: unrestricted
diff --git a/tool_collections/samtools/samtools_bedcov/.shed.yml b/tool_collections/samtools/samtools_bedcov/.shed.yml
index cb1b2636a41..dd880132524 100644
--- a/tool_collections/samtools/samtools_bedcov/.shed.yml
+++ b/tool_collections/samtools/samtools_bedcov/.shed.yml
@@ -7,5 +7,5 @@ long_description: |
 name: samtools_bedcov
 owner: devteam
 homepage_url: http://www.htslib.org/
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_bedcov
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/samtools_bedcov
 type: unrestricted
diff --git a/tool_collections/samtools/samtools_calmd/.shed.yml b/tool_collections/samtools/samtools_calmd/.shed.yml
index 73d004debcf..5a33b501fc9 100644
--- a/tool_collections/samtools/samtools_calmd/.shed.yml
+++ b/tool_collections/samtools/samtools_calmd/.shed.yml
@@ -6,5 +6,5 @@ long_description: |
 name: samtools_calmd
 owner: devteam
 homepage_url: http://www.htslib.org/
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_calmd
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/samtools_calmd
 type: unrestricted
diff --git a/tool_collections/samtools/samtools_flagstat/.shed.yml b/tool_collections/samtools/samtools_flagstat/.shed.yml
index 9e42571d998..8b20fb87320 100644
--- a/tool_collections/samtools/samtools_flagstat/.shed.yml
+++ b/tool_collections/samtools/samtools_flagstat/.shed.yml
@@ -7,5 +7,5 @@ long_description: |
 name: samtools_flagstat
 owner: devteam
 homepage_url: http://www.htslib.org/
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_flagstat
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/samtools_flagstat
 type: unrestricted
diff --git a/tool_collections/samtools/samtools_idxstats/.shed.yml b/tool_collections/samtools/samtools_idxstats/.shed.yml
index 640eecf9ffd..445ef516ffc 100644
--- a/tool_collections/samtools/samtools_idxstats/.shed.yml
+++ b/tool_collections/samtools/samtools_idxstats/.shed.yml
@@ -8,5 +8,5 @@ long_description: |
 name: samtools_idxstats
 owner: devteam
 homepage_url: http://www.htslib.org/
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_idxstats
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/samtools_idxstats
 type: unrestricted
diff --git a/tool_collections/samtools/samtools_mpileup/.shed.yml b/tool_collections/samtools/samtools_mpileup/.shed.yml
index 19d7b16c98c..513cf55e3d3 100644
--- a/tool_collections/samtools/samtools_mpileup/.shed.yml
+++ b/tool_collections/samtools/samtools_mpileup/.shed.yml
@@ -9,5 +9,5 @@ long_description: |
 name: samtools_mpileup
 owner: devteam
 homepage_url: http://www.htslib.org/
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_mpileup
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/samtools_mpileup
 type: unrestricted
diff --git a/tool_collections/samtools/samtools_phase/.shed.yml b/tool_collections/samtools/samtools_phase/.shed.yml
index da1b900371a..39b64cb878e 100644
--- a/tool_collections/samtools/samtools_phase/.shed.yml
+++ b/tool_collections/samtools/samtools_phase/.shed.yml
@@ -5,5 +5,5 @@ description: Call and phase heterozygous SNPs
 name: samtools_phase
 owner: devteam
 homepage_url: http://www.htslib.org/
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_phase
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/samtools_phase
 type: unrestricted
diff --git a/tool_collections/samtools/samtools_reheader/.shed.yml b/tool_collections/samtools/samtools_reheader/.shed.yml
index 4d7a5323f2c..3ca0be0fea6 100644
--- a/tool_collections/samtools/samtools_reheader/.shed.yml
+++ b/tool_collections/samtools/samtools_reheader/.shed.yml
@@ -6,5 +6,5 @@ long_description: |
 name: samtools_reheader
 owner: devteam
 homepage_url: http://www.htslib.org/
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_reheader
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/samtools_reheader
 type: unrestricted
diff --git a/tool_collections/samtools/samtools_rmdup/.shed.yml b/tool_collections/samtools/samtools_rmdup/.shed.yml
index 90dd377e794..f8fd401af27 100644
--- a/tool_collections/samtools/samtools_rmdup/.shed.yml
+++ b/tool_collections/samtools/samtools_rmdup/.shed.yml
@@ -10,5 +10,5 @@ long_description: |
 name: samtools_rmdup
 owner: devteam
 homepage_url: http://www.htslib.org/
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_rmdup
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/samtools_rmdup
 type: unrestricted
diff --git a/tool_collections/samtools/samtools_slice_bam/.shed.yml b/tool_collections/samtools/samtools_slice_bam/.shed.yml
index 9b7fa53e074..c305a5b2852 100644
--- a/tool_collections/samtools/samtools_slice_bam/.shed.yml
+++ b/tool_collections/samtools/samtools_slice_bam/.shed.yml
@@ -7,5 +7,5 @@ long_description: |
 name: samtools_slice_bam
 owner: devteam
 homepage_url: http://www.htslib.org/
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_slice_bam
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/samtools_slice_bam
 type: unrestricted
diff --git a/tool_collections/samtools/samtools_sort/.shed.yml b/tool_collections/samtools/samtools_sort/.shed.yml
index 1ad7582aae7..db06ec5bcec 100644
--- a/tool_collections/samtools/samtools_sort/.shed.yml
+++ b/tool_collections/samtools/samtools_sort/.shed.yml
@@ -7,5 +7,5 @@ long_description: |
 name: samtools_sort
 owner: devteam
 homepage_url: http://www.htslib.org/
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_sort
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/samtools_sort
 type: unrestricted
diff --git a/tool_collections/samtools/samtools_split/.shed.yml b/tool_collections/samtools/samtools_split/.shed.yml
index 67d0210b75e..3dcb599b5ef 100644
--- a/tool_collections/samtools/samtools_split/.shed.yml
+++ b/tool_collections/samtools/samtools_split/.shed.yml
@@ -7,5 +7,5 @@ long_description: |
 name: samtools_split
 owner: devteam
 homepage_url: http://www.htslib.org/
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_split
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/samtools_split
 type: unrestricted
diff --git a/tool_collections/samtools/samtools_stats/.shed.yml b/tool_collections/samtools/samtools_stats/.shed.yml
index 200ac5f35ee..2e71c973eb1 100644
--- a/tool_collections/samtools/samtools_stats/.shed.yml
+++ b/tool_collections/samtools/samtools_stats/.shed.yml
@@ -8,5 +8,5 @@ long_description: |
 name: samtools_stats
 owner: devteam
 homepage_url: http://www.htslib.org/
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/samtools/samtools_stats
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/samtools_stats
 type: unrestricted

From c345e50001556f8cf9738925d307768ffe25d710 Mon Sep 17 00:00:00 2001
From: Martin Cech <cech.marten@gmail.com>
Date: Tue, 24 Jan 2017 12:58:39 -0500
Subject: [PATCH 04/40] set chunk size to 4 temporarily

---
 .travis.yml | 4 +++-
 1 file changed, 3 insertions(+), 1 deletion(-)

diff --git a/.travis.yml b/.travis.yml
index d4ea00e6e4e..a3b305de373 100644
--- a/.travis.yml
+++ b/.travis.yml
@@ -6,6 +6,8 @@ python: 2.7
 env:
   - CHUNK=0
   - CHUNK=1
+  - CHUNK=2
+  - CHUNK=3
 
 before_install:
   - export GALAXY_REPO=https://github.com/galaxyproject/galaxy
@@ -32,7 +34,7 @@ install:
       planemo ci_find_repos --exclude_from .tt_blacklist \
                             --exclude packages --exclude data_managers \
                             --changed_in_commit_range "$TRAVIS_COMMIT_RANGE" \
-                            --chunk_count 2 --chunk "${CHUNK}" \
+                            --chunk_count 4 --chunk "${CHUNK}" \
                             --output changed_repositories_chunk.list
   - cat changed_repositories_chunk.list
 

From 2a298cbedeca57204bb1961c2004ecd26563683e Mon Sep 17 00:00:00 2001
From: Martin Cech <cech.marten@gmail.com>
Date: Tue, 24 Jan 2017 13:47:13 -0500
Subject: [PATCH 05/40] fix samtools stats schema lint

---
 .../samtools/samtools_stats/samtools_stats.xml    | 15 ++++++++-------
 1 file changed, 8 insertions(+), 7 deletions(-)

diff --git a/tool_collections/samtools/samtools_stats/samtools_stats.xml b/tool_collections/samtools/samtools_stats/samtools_stats.xml
index ab360820353..7cef610f0a3 100644
--- a/tool_collections/samtools/samtools_stats/samtools_stats.xml
+++ b/tool_collections/samtools/samtools_stats/samtools_stats.xml
@@ -195,15 +195,15 @@
         <param name="insert_size" type="integer" value="8000" label="Maximum insert size" help="--insert-size; default = 8000"/>
 
         <!--
-            
+
             The -I option of samtools stats returns the following message in version 1.2:
 
             Samtools-htslib: init_group_id() header parsing not yet implemented
             Abort trap: 6
-    
+
             Because of this the section below is commented out until this stats bug is fixed
 
-            <param name="read_group" type="select" optional="true" label="Limit to a specific read group name" > 
+            <param name="read_group" type="select" optional="true" label="Limit to a specific read group name" >
                 <options>
                     <filter type="data_meta" ref="input_file" key="read_groups" />
                 </options>
@@ -214,7 +214,7 @@
         <param name="read_length" type="integer" value="0" label="Minimum read length to generate statistics for" help="--read-length; default = no cutoff"/>
         <param name="most_inserts" type="float" value="0.99" label="Report only the main part of inserts" help="--most-inserts; default = 0.99"/>
         <param name="trim_quality" type="integer" value="0" label="BWA trim parameter" help="--trim-quality; default = 0"/>
-        
+
         <conditional name="use_reference">
             <param name="use_ref_selector" type="select" label="Use reference sequence" help="--ref-seq; required for GC-depth and mismatches-per-cycle calculation">
                 <option value="yes">Use reference</option>
@@ -228,8 +228,9 @@
                     </param>
                     <when value="cached">
                         <param name="ref_file" type="select" label="Using genome">
-                            <options from_data_table="fasta_indexes" />
-                            <filter type="data_meta" ref="input_file" key="dbkey" column="1" />
+                            <options from_data_table="fasta_indexes">
+                                <filter type="data_meta" ref="input_file" key="dbkey" column="1" />
+                            </options>
                         </param>
                     </when>
                     <when value="history">
@@ -288,7 +289,7 @@ This tool runs the ``samtools stats`` command. It has the following options::
     -r, --ref-seq <file>                Reference sequence (required for GC-depth and mismatches-per-cycle calculation). Galaxy
                                         will provide options for selecting a reference cached as this Galaxy instance or choosing
                                         one from history.
-   
+
 
     ]]></help>
     <expand macro="citations"></expand>

From 70de720198f2ee531d6720bfd9d8e56a406123a3 Mon Sep 17 00:00:00 2001
From: Martin Cech <cech.marten@gmail.com>
Date: Mon, 30 Jan 2017 14:47:08 -0500
Subject: [PATCH 06/40] remove tool_dependencies.xml for samtools, let conda do
 it

---
 tool_collections/samtools/bam_to_cram/tool_dependencies.xml | 1 -
 tool_collections/samtools/bam_to_sam/tool_dependencies.xml  | 1 -
 tool_collections/samtools/cram_to_bam/tool_dependencies.xml | 1 -
 tool_collections/samtools/sam_to_bam/tool_dependencies.xml  | 1 -
 .../samtools/samtools_bedcov/tool_dependencies.xml          | 1 -
 .../samtools/samtools_calmd/tool_dependencies.xml           | 1 -
 .../samtools/samtools_flagstat/tool_dependencies.xml        | 1 -
 .../samtools/samtools_idxstats/tool_dependencies.xml        | 1 -
 .../samtools/samtools_mpileup/tool_dependencies.xml         | 1 -
 .../samtools/samtools_phase/tool_dependencies.xml           | 1 -
 .../samtools/samtools_reheader/tool_dependencies.xml        | 1 -
 .../samtools/samtools_rmdup/tool_dependencies.xml           | 6 ------
 .../samtools/samtools_slice_bam/tool_dependencies.xml       | 1 -
 .../samtools/samtools_sort/tool_dependencies.xml            | 1 -
 .../samtools/samtools_split/tool_dependencies.xml           | 1 -
 .../samtools/samtools_stats/tool_dependencies.xml           | 1 -
 tool_collections/samtools/tool_dependencies.xml             | 6 ------
 17 files changed, 27 deletions(-)
 delete mode 120000 tool_collections/samtools/bam_to_cram/tool_dependencies.xml
 delete mode 120000 tool_collections/samtools/bam_to_sam/tool_dependencies.xml
 delete mode 120000 tool_collections/samtools/cram_to_bam/tool_dependencies.xml
 delete mode 120000 tool_collections/samtools/sam_to_bam/tool_dependencies.xml
 delete mode 120000 tool_collections/samtools/samtools_bedcov/tool_dependencies.xml
 delete mode 120000 tool_collections/samtools/samtools_calmd/tool_dependencies.xml
 delete mode 120000 tool_collections/samtools/samtools_flagstat/tool_dependencies.xml
 delete mode 120000 tool_collections/samtools/samtools_idxstats/tool_dependencies.xml
 delete mode 120000 tool_collections/samtools/samtools_mpileup/tool_dependencies.xml
 delete mode 120000 tool_collections/samtools/samtools_phase/tool_dependencies.xml
 delete mode 120000 tool_collections/samtools/samtools_reheader/tool_dependencies.xml
 delete mode 100644 tool_collections/samtools/samtools_rmdup/tool_dependencies.xml
 delete mode 120000 tool_collections/samtools/samtools_slice_bam/tool_dependencies.xml
 delete mode 120000 tool_collections/samtools/samtools_sort/tool_dependencies.xml
 delete mode 120000 tool_collections/samtools/samtools_split/tool_dependencies.xml
 delete mode 120000 tool_collections/samtools/samtools_stats/tool_dependencies.xml
 delete mode 100644 tool_collections/samtools/tool_dependencies.xml

diff --git a/tool_collections/samtools/bam_to_cram/tool_dependencies.xml b/tool_collections/samtools/bam_to_cram/tool_dependencies.xml
deleted file mode 120000
index b11b378d63c..00000000000
--- a/tool_collections/samtools/bam_to_cram/tool_dependencies.xml
+++ /dev/null
@@ -1 +0,0 @@
-../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/bam_to_sam/tool_dependencies.xml b/tool_collections/samtools/bam_to_sam/tool_dependencies.xml
deleted file mode 120000
index b11b378d63c..00000000000
--- a/tool_collections/samtools/bam_to_sam/tool_dependencies.xml
+++ /dev/null
@@ -1 +0,0 @@
-../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/cram_to_bam/tool_dependencies.xml b/tool_collections/samtools/cram_to_bam/tool_dependencies.xml
deleted file mode 120000
index b11b378d63c..00000000000
--- a/tool_collections/samtools/cram_to_bam/tool_dependencies.xml
+++ /dev/null
@@ -1 +0,0 @@
-../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/sam_to_bam/tool_dependencies.xml b/tool_collections/samtools/sam_to_bam/tool_dependencies.xml
deleted file mode 120000
index b11b378d63c..00000000000
--- a/tool_collections/samtools/sam_to_bam/tool_dependencies.xml
+++ /dev/null
@@ -1 +0,0 @@
-../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_bedcov/tool_dependencies.xml b/tool_collections/samtools/samtools_bedcov/tool_dependencies.xml
deleted file mode 120000
index b11b378d63c..00000000000
--- a/tool_collections/samtools/samtools_bedcov/tool_dependencies.xml
+++ /dev/null
@@ -1 +0,0 @@
-../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_calmd/tool_dependencies.xml b/tool_collections/samtools/samtools_calmd/tool_dependencies.xml
deleted file mode 120000
index b11b378d63c..00000000000
--- a/tool_collections/samtools/samtools_calmd/tool_dependencies.xml
+++ /dev/null
@@ -1 +0,0 @@
-../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_flagstat/tool_dependencies.xml b/tool_collections/samtools/samtools_flagstat/tool_dependencies.xml
deleted file mode 120000
index b11b378d63c..00000000000
--- a/tool_collections/samtools/samtools_flagstat/tool_dependencies.xml
+++ /dev/null
@@ -1 +0,0 @@
-../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_idxstats/tool_dependencies.xml b/tool_collections/samtools/samtools_idxstats/tool_dependencies.xml
deleted file mode 120000
index b11b378d63c..00000000000
--- a/tool_collections/samtools/samtools_idxstats/tool_dependencies.xml
+++ /dev/null
@@ -1 +0,0 @@
-../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_mpileup/tool_dependencies.xml b/tool_collections/samtools/samtools_mpileup/tool_dependencies.xml
deleted file mode 120000
index b11b378d63c..00000000000
--- a/tool_collections/samtools/samtools_mpileup/tool_dependencies.xml
+++ /dev/null
@@ -1 +0,0 @@
-../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_phase/tool_dependencies.xml b/tool_collections/samtools/samtools_phase/tool_dependencies.xml
deleted file mode 120000
index b11b378d63c..00000000000
--- a/tool_collections/samtools/samtools_phase/tool_dependencies.xml
+++ /dev/null
@@ -1 +0,0 @@
-../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_reheader/tool_dependencies.xml b/tool_collections/samtools/samtools_reheader/tool_dependencies.xml
deleted file mode 120000
index b11b378d63c..00000000000
--- a/tool_collections/samtools/samtools_reheader/tool_dependencies.xml
+++ /dev/null
@@ -1 +0,0 @@
-../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_rmdup/tool_dependencies.xml b/tool_collections/samtools/samtools_rmdup/tool_dependencies.xml
deleted file mode 100644
index 03998a77628..00000000000
--- a/tool_collections/samtools/samtools_rmdup/tool_dependencies.xml
+++ /dev/null
@@ -1,6 +0,0 @@
-<?xml version="1.0"?>
-<tool_dependency>
-    <package name="samtools" version="0.1.19">
-        <repository name="package_samtools_0_1_19" owner="iuc" />
-    </package>
-</tool_dependency>
diff --git a/tool_collections/samtools/samtools_slice_bam/tool_dependencies.xml b/tool_collections/samtools/samtools_slice_bam/tool_dependencies.xml
deleted file mode 120000
index b11b378d63c..00000000000
--- a/tool_collections/samtools/samtools_slice_bam/tool_dependencies.xml
+++ /dev/null
@@ -1 +0,0 @@
-../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_sort/tool_dependencies.xml b/tool_collections/samtools/samtools_sort/tool_dependencies.xml
deleted file mode 120000
index b11b378d63c..00000000000
--- a/tool_collections/samtools/samtools_sort/tool_dependencies.xml
+++ /dev/null
@@ -1 +0,0 @@
-../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_split/tool_dependencies.xml b/tool_collections/samtools/samtools_split/tool_dependencies.xml
deleted file mode 120000
index b11b378d63c..00000000000
--- a/tool_collections/samtools/samtools_split/tool_dependencies.xml
+++ /dev/null
@@ -1 +0,0 @@
-../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/samtools_stats/tool_dependencies.xml b/tool_collections/samtools/samtools_stats/tool_dependencies.xml
deleted file mode 120000
index b11b378d63c..00000000000
--- a/tool_collections/samtools/samtools_stats/tool_dependencies.xml
+++ /dev/null
@@ -1 +0,0 @@
-../tool_dependencies.xml
\ No newline at end of file
diff --git a/tool_collections/samtools/tool_dependencies.xml b/tool_collections/samtools/tool_dependencies.xml
deleted file mode 100644
index 8995249fe5f..00000000000
--- a/tool_collections/samtools/tool_dependencies.xml
+++ /dev/null
@@ -1,6 +0,0 @@
-<?xml version="1.0"?>
-<tool_dependency>
-    <package name="samtools" version="1.2">
-        <repository name="package_samtools_1_2" owner="iuc" />
-    </package>
-</tool_dependency>

From b392dc31f29125d7e97b2a0ff5289f51beca6db6 Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Mon, 6 Feb 2017 10:52:41 +0100
Subject: [PATCH 07/40] samtools linting errors

---
 .../samtools/bam_to_sam/bam_to_sam.xml        | 28 ++++++++++---------
 1 file changed, 15 insertions(+), 13 deletions(-)

diff --git a/tool_collections/samtools/bam_to_sam/bam_to_sam.xml b/tool_collections/samtools/bam_to_sam/bam_to_sam.xml
index faa8c97dbc1..ed53bad7cfb 100644
--- a/tool_collections/samtools/bam_to_sam/bam_to_sam.xml
+++ b/tool_collections/samtools/bam_to_sam/bam_to_sam.xml
@@ -1,21 +1,23 @@
 <tool id="bam_to_sam" name="BAM-to-SAM" version="2.0">
+  <description>convert BAM to SAM</description>  
+
   <macros>
     <import>macros.xml</import>
   </macros>
   <expand macro="requirements"></expand>
-  <expand macro="version_command"></expand>
   <expand macro="stdio"></expand>
-  <description>convert BAM to SAM</description>  
-    <command>
-<![CDATA[
-  samtools view -o "${output1}" ${header} "${input1}"
-]]>
-    </command>
+  <expand macro="version_command"></expand>
+
+  <command><![CDATA[
+    samtools view -o '${output1}' ${header} '${input1}'
+  ]]></command>
+
     <inputs>
-        <param format="bam" label="BAM File to Convert" name="input1" type="data" />
-        <param name="header" label="Header options" type="select" help="Allows to choose between seeing the entire dataset with the header, header only, or data only.">
+        <param format="bam" name="input1" type="data" label="BAM File to Convert" />
+        <param name="header" label="Header options" type="select"
+               help="Allows to choose between seeing the entire dataset with the header, header only, or data only.">
           <option value="-h">Include header in SAM output (-h)</option>
-          <option value="-H">Print header only (-H)</option>
+          <option value="-H">Return header only (-H)</option>
           <option value="">Exclude header</option>
         </param>
     </inputs>
@@ -26,17 +28,17 @@
         <test>
             <param ftype="bam" name="input1" value="bam_to_sam_in1.bam" />
             <param name="header" value="-h" />
-            <output file="bam_to_sam_out1.sam" name="output1" sorted="True" />
+            <output file="bam_to_sam_out1.sam" name="output1" />
         </test>
         <test>
             <param ftype="bam" name="input1" value="bam_to_sam_in1.bam" />
             <param name="header" value="-H" />
-            <output file="bam_to_sam_out2.sam" name="output1" sorted="True" />
+            <output file="bam_to_sam_out2.sam" name="output1" />
         </test>
         <test>
             <param ftype="bam" name="input1" value="bam_to_sam_in1.bam" />
             <param name="header" value="" />
-            <output file="bam_to_sam_out3.sam" name="output1" sorted="True" />
+            <output file="bam_to_sam_out3.sam" name="output1" />
         </test>
     </tests>
     <help>

From c5da921578f509bce70e448698814f8e997f79ed Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Mon, 6 Feb 2017 11:07:22 +0100
Subject: [PATCH 08/40] samtools mpileup - attempt to get testing and linting
 working

---
 .../samtools_mpileup/samtools_mpileup.xml     | 61 ++++++++++---------
 1 file changed, 33 insertions(+), 28 deletions(-)

diff --git a/tool_collections/samtools/samtools_mpileup/samtools_mpileup.xml b/tool_collections/samtools/samtools_mpileup/samtools_mpileup.xml
index 35fecf8cf42..6f731d5601f 100644
--- a/tool_collections/samtools/samtools_mpileup/samtools_mpileup.xml
+++ b/tool_collections/samtools/samtools_mpileup/samtools_mpileup.xml
@@ -1,5 +1,5 @@
 <tool id="samtools_mpileup" name="MPileup" version="2.1.2">
-    <description>call variants</description>
+    <description>multi-way pileup of variants</description>
     <macros>
         <import>macros.xml</import>
     </macros>
@@ -12,11 +12,14 @@
         ln -s "${input_bam}" "localbam_${bam_count}.bam" &&
         ln -s "${input_bam.metadata.bam_index}" "localbam_${bam_count}.bam.bai" &&
     #end for
+
     #if $reference_source.reference_source_selector == "history":
-       ln -s "${reference_source.ref_file}" && samtools faidx `basename "${reference_source.ref_file}"` && samtools mpileup
-    #else:
-        samtools mpileup
+       ln -s "${reference_source.ref_file}" &&
+       samtools faidx `basename "${reference_source.ref_file}"` &&
     #end if
+
+    samtools mpileup
+
     #if $reference_source.reference_source_selector != "history":
         -f "${reference_source.ref_file.fields.path}"
     #else:
@@ -90,6 +93,32 @@
     --output "$output_mpileup" 2> "$output_log"
     ]]>
     </command>
+
+    <configfiles>
+        <configfile name="excluded_read_groups">
+<![CDATA[
+#set pasted_data = ''
+#if str( $advanced_options.advanced_options_selector ) == "advanced":
+    #if str( $advanced_options.exclude_read_group.exclude_read_groups ) == "paste":
+        #set pasted_data = '\t'.join( str( $advanced_options.exclude_read_group['read_groups'] ).split() )
+    #end if
+#end if
+${pasted_data}
+]]>
+        </configfile>
+        <configfile name="pasted_regions">
+<![CDATA[
+#set pasted_data = ''
+#if str( $advanced_options.advanced_options_selector ) == "advanced":
+    #if str( $advanced_options.limit_by_region.limit_by_regions ) == "paste":
+        #set pasted_data = '\t'.join( str( $advanced_options.limit_by_region['region_paste'] ).split() )
+    #end if
+#end if
+${pasted_data}
+]]>
+        </configfile>
+    </configfiles>
+
     <inputs>
         <conditional name="reference_source">
             <param label="Choose the source for the reference genome" name="reference_source_selector" type="select">
@@ -344,29 +373,5 @@ Report variants for one or multiple BAM files. Alignment records are grouped by
 **Notes**: Assuming diploid individuals.
 ]]>
     </help>
-    <configfiles>
-        <configfile name="excluded_read_groups">
-<![CDATA[
-#set pasted_data = ''
-#if str( $advanced_options.advanced_options_selector ) == "advanced":
-    #if str( $advanced_options.exclude_read_group.exclude_read_groups ) == "paste":
-        #set pasted_data = '\t'.join( str( $advanced_options.exclude_read_group['read_groups'] ).split() )
-    #end if
-#end if
-${pasted_data}
-]]>
-        </configfile>
-        <configfile name="pasted_regions">
-<![CDATA[
-#set pasted_data = ''
-#if str( $advanced_options.advanced_options_selector ) == "advanced":
-    #if str( $advanced_options.limit_by_region.limit_by_regions ) == "paste":
-        #set pasted_data = '\t'.join( str( $advanced_options.limit_by_region['region_paste'] ).split() )
-    #end if
-#end if
-${pasted_data}
-]]>
-        </configfile>
-    </configfiles>
     <expand macro="citations" />
 </tool>

From ae39c24fc2f8578b5248db56fb5c8b208b309d18 Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Mon, 6 Feb 2017 11:11:10 +0100
Subject: [PATCH 09/40] samtools mpileup - attempt 2 to get testing and linting
 working

---
 .../samtools/samtools_mpileup/samtools_mpileup.xml            | 4 ++--
 .../samtools_mpileup/test-data/samtools_mpileup_out_1.log.txt | 3 +++
 .../samtools_mpileup/test-data/samtools_mpileup_out_2.log.txt | 3 +++
 3 files changed, 8 insertions(+), 2 deletions(-)
 create mode 100644 tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_1.log.txt
 create mode 100644 tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_2.log.txt

diff --git a/tool_collections/samtools/samtools_mpileup/samtools_mpileup.xml b/tool_collections/samtools/samtools_mpileup/samtools_mpileup.xml
index 6f731d5601f..8490749cb8b 100644
--- a/tool_collections/samtools/samtools_mpileup/samtools_mpileup.xml
+++ b/tool_collections/samtools/samtools_mpileup/samtools_mpileup.xml
@@ -294,7 +294,7 @@ ${pasted_data}
             <param name="base_position_on_reads" value="true" />
             <param name="output_mapping_quality" value="true" />
             <output file="samtools_mpileup_out_1.pileup" name="output_mpileup" />
-            <output file="samtools_mpileup_out_1.log" name="output_log" />
+            <output file="samtools_mpileup_out_1.log.txt" name="output_log" />
         </test>
         <test>
             <param name="reference_source_selector" value="history" />
@@ -310,7 +310,7 @@ ${pasted_data}
             <param name="advanced_options_selector" value="basic" />
             <param name="genotype_likelihood_computation_type|output_format" value="VCF" />
             <output file="samtools_mpileup_out_2.vcf" ftype="vcf" lines_diff="8" name="output_mpileup" />
-            <output file="samtools_mpileup_out_2.log" name="output_log" />
+            <output file="samtools_mpileup_out_2.log.txt" name="output_log" />
         </test>
     </tests>
     <help>
diff --git a/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_1.log.txt b/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_1.log.txt
new file mode 100644
index 00000000000..43ec7ed1b9d
--- /dev/null
+++ b/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_1.log.txt
@@ -0,0 +1,3 @@
+[fai_load] build FASTA index.
+[mpileup] 1 samples in 1 input files
+<mpileup> Set max per-file depth to 8000
diff --git a/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_2.log.txt b/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_2.log.txt
new file mode 100644
index 00000000000..43ec7ed1b9d
--- /dev/null
+++ b/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_2.log.txt
@@ -0,0 +1,3 @@
+[fai_load] build FASTA index.
+[mpileup] 1 samples in 1 input files
+<mpileup> Set max per-file depth to 8000

From b637cef54476235d718ec87dc0c2c27781fce72f Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Mon, 6 Feb 2017 11:48:56 +0100
Subject: [PATCH 10/40] samtools stats+sam_to_bam - small changes to get tests
 working

---
 tool_collections/samtools/sam_to_bam/sam_to_bam.xml | 12 ++++++------
 .../samtools/samtools_stats/samtools_stats.xml      | 13 +++++--------
 2 files changed, 11 insertions(+), 14 deletions(-)

diff --git a/tool_collections/samtools/sam_to_bam/sam_to_bam.xml b/tool_collections/samtools/sam_to_bam/sam_to_bam.xml
index de35dbe6998..183ce2fa66a 100644
--- a/tool_collections/samtools/sam_to_bam/sam_to_bam.xml
+++ b/tool_collections/samtools/sam_to_bam/sam_to_bam.xml
@@ -9,13 +9,13 @@
   <command>
     <![CDATA[
         #if $source.index_source == "history":
-          ln -s $source.ref_file input.fa &&
+          ln -s '${source.ref_file}' input.fa &&
           samtools faidx input.fa &&
         #else
-          ln -s ${source.index.fields.path} input.fa &&
-          ln -s ${source.index.fields.path}.fai input.fa.fai &&
+          ln -s '${source.index.fields.path}' input.fa &&
+          ln -s '${source.index.fields.path}.fai' input.fa.fai &&
         #end if
-        samtools view -b -@ \${GALAXY_SLOTS:-1} -t input.fa.fai "$input1" | samtools sort -O bam -@ \${GALAXY_SLOTS:-1} -o "$output1" -T temp
+        samtools view -b -@ \${GALAXY_SLOTS:-1} -t input.fa.fai '$input1' | samtools sort -O bam -@ \${GALAXY_SLOTS:-1} -o '$output1' -T temp
     ]]>
     </command>
     <inputs>
@@ -25,7 +25,7 @@
                 <option value="history">Use a genome from the history</option>
             </param>
             <when value="cached">
-                <param format="sam" label="SAM file to convert" metadata_name="dbkey" name="input1" type="data">
+                <param format="sam" label="SAM file to convert" name="input1" type="data">
                     <validator type="unspecified_build" />
                     <validator message="Sequences are not currently available for the specified build." metadata_column="1" metadata_name="dbkey" table_name="fasta_indexes" type="dataset_metadata_in_data_table" />
                 </param>
@@ -38,7 +38,7 @@
             </when>
             <when value="history">
                 <param format="sam" label="SAM file to convert" name="input1" type="data" />
-                <param format="fasta" label="Using reference file" metadata_name="dbkey" name="ref_file" type="data" />
+                <param format="fasta" label="Using reference file" name="ref_file" type="data" />
             </when>
         </conditional>
     </inputs>
diff --git a/tool_collections/samtools/samtools_stats/samtools_stats.xml b/tool_collections/samtools/samtools_stats/samtools_stats.xml
index 7cef610f0a3..83408f663d8 100644
--- a/tool_collections/samtools/samtools_stats/samtools_stats.xml
+++ b/tool_collections/samtools/samtools_stats/samtools_stats.xml
@@ -9,13 +9,11 @@
     <command><![CDATA[
         #if $use_reference.use_ref_selector == "yes":
             #if $use_reference.reference_source.reference_source_selector == "history":
-               ln -s "${use_reference.reference_source.ref_file}" && samtools faidx `basename "${use_reference.reference_source.ref_file}"` && samtools stats
-            #else:
-                samtools stats
+               ln -s "${use_reference.reference_source.ref_file}" && samtools faidx `basename "${use_reference.reference_source.ref_file}"` &&
             #end if
-        #else:
-            samtools stats
         #end if
+        
+        samtools stats
         "${input_file}"
         --coverage ${coverage_min},${coverage_max},${coverage_step}
         ${remove_dups}
@@ -55,7 +53,8 @@
             && mkdir split && echo ${split_output.generate_tables} &&
 
             #if 'sn' in $outputs_to_split:
-                echo "# Summary Numbers\n"  > "split/Summary numbers.tab" &&
+                echo "# Summary Numbers" > "split/Summary numbers.tab" &&
+                echo "" >> "split/Summary numbers.tab" &&
                 grep -q ^SN  "${output}" ; if [ $? = 0 ] ; then grep ^SN  "${output}" | cut -f 2- >> "split/Summary numbers.tab"  ; fi &&
             #end if
 
@@ -289,8 +288,6 @@ This tool runs the ``samtools stats`` command. It has the following options::
     -r, --ref-seq <file>                Reference sequence (required for GC-depth and mismatches-per-cycle calculation). Galaxy
                                         will provide options for selecting a reference cached as this Galaxy instance or choosing
                                         one from history.
-
-
     ]]></help>
     <expand macro="citations"></expand>
 </tool>

From eee57a19ac0e2ff3f8aa0d34bcf22876e0878f33 Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Wed, 15 Feb 2017 12:02:45 +0100
Subject: [PATCH 11/40] Samtools: update to version_command

---
 tool_collections/samtools/macros.xml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/tool_collections/samtools/macros.xml b/tool_collections/samtools/macros.xml
index 649aa7bb054..72482212f92 100644
--- a/tool_collections/samtools/macros.xml
+++ b/tool_collections/samtools/macros.xml
@@ -42,7 +42,7 @@
         </citations>
     </xml>
     <xml name="version_command">
-        <version_command>echo $(samtools --version | head -n 1)", "$(samtools --version | grep -o -E "htslib.*?")</version_command>
+        <version_command>samtools 2>&1 | grep Version</version_command>
     </xml>
     <xml name="stdio">
         <stdio>

From 840024e2154da730bfffad5948f015d4e0a9b132 Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Wed, 15 Feb 2017 12:21:30 +0100
Subject: [PATCH 12/40] Samtools: update to version_command - attempt 2

---
 tool_collections/samtools/macros.xml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/tool_collections/samtools/macros.xml b/tool_collections/samtools/macros.xml
index 72482212f92..a4e6d47a246 100644
--- a/tool_collections/samtools/macros.xml
+++ b/tool_collections/samtools/macros.xml
@@ -42,7 +42,7 @@
         </citations>
     </xml>
     <xml name="version_command">
-        <version_command>samtools 2>&1 | grep Version</version_command>
+        <version_command>samtools 2&gt;&amp;1 | grep Version</version_command>
     </xml>
     <xml name="stdio">
         <stdio>

From eaf496a1d6d2be768a809f711819d4fc05b90918 Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Wed, 15 Feb 2017 12:48:09 +0100
Subject: [PATCH 13/40] kraken: minor changes

---
 tool_collections/kraken/kraken_report/kraken-report.xml | 2 +-
 tool_collections/kraken/macros.xml                      | 3 +--
 2 files changed, 2 insertions(+), 3 deletions(-)

diff --git a/tool_collections/kraken/kraken_report/kraken-report.xml b/tool_collections/kraken/kraken_report/kraken-report.xml
index c4cc134c408..2883566e151 100644
--- a/tool_collections/kraken/kraken_report/kraken-report.xml
+++ b/tool_collections/kraken/kraken_report/kraken-report.xml
@@ -12,7 +12,7 @@
     <command>
         <![CDATA[
         @SET_DATABASE_PATH@ &&
-        kraken-report @INPUT_DATABASE@ "${kraken_output}" > "$output_report"
+        kraken-report @INPUT_DATABASE@ '${kraken_output}' > '$output_report'
         ]]>
     </command>
     <inputs>
diff --git a/tool_collections/kraken/macros.xml b/tool_collections/kraken/macros.xml
index 0943abbbe5e..d851dd80594 100644
--- a/tool_collections/kraken/macros.xml
+++ b/tool_collections/kraken/macros.xml
@@ -3,7 +3,6 @@
     <xml name="requirements">
         <requirements>
             <requirement type="package" version="0.10.6_eaf8fb68">kraken</requirement>
-            <requirement type="package" version="0.10.6-eaf8fb68">kraken</requirement>
         </requirements>
     </xml>
     <xml name="stdio">
@@ -28,4 +27,4 @@
     </xml>
     <token name="@INPUT_DATABASE@">--db ${kraken_database.fields.name}</token>
     <token name="@SET_DATABASE_PATH@">export KRAKEN_DB_PATH="${kraken_database.fields.path}"</token>
-</macros>
\ No newline at end of file
+</macros>

From d99fc2575bfb82e9e84d6aeedb0fd36db5a62b23 Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Wed, 15 Feb 2017 12:49:24 +0100
Subject: [PATCH 14/40] Kraken: removal of tool-dependencies

---
 tool_collections/kraken/kraken/tool_dependencies.xml        | 6 ------
 tool_collections/kraken/kraken_filter/tool_dependencies.xml | 6 ------
 tool_collections/kraken/kraken_report/tool_dependencies.xml | 6 ------
 .../kraken/kraken_translate/tool_dependencies.xml           | 6 ------
 4 files changed, 24 deletions(-)
 delete mode 100644 tool_collections/kraken/kraken/tool_dependencies.xml
 delete mode 100644 tool_collections/kraken/kraken_filter/tool_dependencies.xml
 delete mode 100644 tool_collections/kraken/kraken_report/tool_dependencies.xml
 delete mode 100644 tool_collections/kraken/kraken_translate/tool_dependencies.xml

diff --git a/tool_collections/kraken/kraken/tool_dependencies.xml b/tool_collections/kraken/kraken/tool_dependencies.xml
deleted file mode 100644
index 04f55a93faf..00000000000
--- a/tool_collections/kraken/kraken/tool_dependencies.xml
+++ /dev/null
@@ -1,6 +0,0 @@
-<?xml version="1.0"?>
-<tool_dependency>
-  <package name="kraken" version="0.10.6-eaf8fb68">
-      <repository name="package_kraken_0_10_6_eaf8fb68" owner="iuc" />
-    </package>
-</tool_dependency>
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_filter/tool_dependencies.xml b/tool_collections/kraken/kraken_filter/tool_dependencies.xml
deleted file mode 100644
index 04f55a93faf..00000000000
--- a/tool_collections/kraken/kraken_filter/tool_dependencies.xml
+++ /dev/null
@@ -1,6 +0,0 @@
-<?xml version="1.0"?>
-<tool_dependency>
-  <package name="kraken" version="0.10.6-eaf8fb68">
-      <repository name="package_kraken_0_10_6_eaf8fb68" owner="iuc" />
-    </package>
-</tool_dependency>
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_report/tool_dependencies.xml b/tool_collections/kraken/kraken_report/tool_dependencies.xml
deleted file mode 100644
index 04f55a93faf..00000000000
--- a/tool_collections/kraken/kraken_report/tool_dependencies.xml
+++ /dev/null
@@ -1,6 +0,0 @@
-<?xml version="1.0"?>
-<tool_dependency>
-  <package name="kraken" version="0.10.6-eaf8fb68">
-      <repository name="package_kraken_0_10_6_eaf8fb68" owner="iuc" />
-    </package>
-</tool_dependency>
\ No newline at end of file
diff --git a/tool_collections/kraken/kraken_translate/tool_dependencies.xml b/tool_collections/kraken/kraken_translate/tool_dependencies.xml
deleted file mode 100644
index 04f55a93faf..00000000000
--- a/tool_collections/kraken/kraken_translate/tool_dependencies.xml
+++ /dev/null
@@ -1,6 +0,0 @@
-<?xml version="1.0"?>
-<tool_dependency>
-  <package name="kraken" version="0.10.6-eaf8fb68">
-      <repository name="package_kraken_0_10_6_eaf8fb68" owner="iuc" />
-    </package>
-</tool_dependency>
\ No newline at end of file

From 9c888807662f5f6d1e38f88dc1f011c46ae0b871 Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Wed, 15 Feb 2017 13:07:31 +0100
Subject: [PATCH 15/40] samtools bam_to_cram small changes

---
 .../bam_to_cram/samtools_bam_to_cram.xml      | 22 ++++++++++---------
 1 file changed, 12 insertions(+), 10 deletions(-)

diff --git a/tool_collections/samtools/bam_to_cram/samtools_bam_to_cram.xml b/tool_collections/samtools/bam_to_cram/samtools_bam_to_cram.xml
index 87838f8347c..91eb67e24b3 100644
--- a/tool_collections/samtools/bam_to_cram/samtools_bam_to_cram.xml
+++ b/tool_collections/samtools/bam_to_cram/samtools_bam_to_cram.xml
@@ -5,8 +5,8 @@
         <import>macros.xml</import>
     </macros>
     <expand macro="requirements"></expand>
-    <expand macro="version_command"></expand>
     <expand macro="stdio"></expand>
+    <expand macro="version_command"></expand>
 
     <command>
     <![CDATA[
@@ -14,8 +14,8 @@
             ln
                 -f
                 -s
-                "${input_alignment.metadata.bam_index}"
-                "${input_alignment}.bai"
+                '${input_alignment.metadata.bam_index}'
+                '${input_alignment}.bai'
                 &&
         #end if
 
@@ -24,14 +24,15 @@
                 -L "${parameter_regions.regions_bed_file}"
             #end if
             
-            -@\${GALAXY_SLOTS:-2}
+            -@\${GALAXY_SLOTS:-1}
             -C
-            -T "${reference_source.input_reference}"
-            -o "$output_alignment"
-            "$input_alignment"
-            
+            -T '${reference_source.input_reference}'
+            -o '${output_alignment}'
+
+            '${input_alignment}'
+
             #if $parameter_regions.target_region == "region"
-                "${parameter_regions.region_string}"
+                '${parameter_regions.region_string}'
             #end if
     ]]>
     </command>
@@ -66,7 +67,8 @@
                 <param name="region_string" type="text" label="Samtools: region in which pileup is generated" help="e.g. chrX or chr:start-end" />
             </when>
             <when value="regions_bed_file">
-                <param name="regions_bed_file" type="data" format="bed" label="only include reads overlapping this BED FILE" help="-L"/>
+                <param name="regions_bed_file" type="data" format="bed"
+                       label="only include reads overlapping this BED FILE" argument="-L"/>
             </when>
         </conditional>
     </inputs>

From 223a8ac66dd2de9d04afd52322990c34c2dbf349 Mon Sep 17 00:00:00 2001
From: Martin Cech <cech.marten@gmail.com>
Date: Fri, 17 Feb 2017 15:55:52 -0500
Subject: [PATCH 16/40] add changes from @nsoranzo

---
 tool_collections/kraken/kraken/kraken.xml     | 31 +++++--------------
 .../kraken_report/kraken-mpa-report.xml       |  6 +---
 2 files changed, 8 insertions(+), 29 deletions(-)

diff --git a/tool_collections/kraken/kraken/kraken.xml b/tool_collections/kraken/kraken/kraken.xml
index 6064c451809..ee995b2e344 100644
--- a/tool_collections/kraken/kraken/kraken.xml
+++ b/tool_collections/kraken/kraken/kraken.xml
@@ -12,8 +12,8 @@
     <command>
         <![CDATA[
         @SET_DATABASE_PATH@ &&
-        kraken --threads \${GALAXY_SLOTS:-1} @INPUT_DATABASE@ 
-        
+        kraken --threads \${GALAXY_SLOTS:-1} @INPUT_DATABASE@
+
         ${only_classified_output}
 
         #if str( $quick_operation.quick ) == "yes":
@@ -22,13 +22,13 @@
 
         #end if
 
-        #if $single_paired.single_paired_selector == "yes":
+        #if $single_paired.single_paired.single_paired_selector == 'yes'
             #if $forward_input.is_of_type( 'fastq' ):
                 --fastq-input
             #else:
                 --fasta-input
             #end if
-            "$forward_input" "$reverse_input"
+            '${single_paired.forward_input}' '${single_paired.reverse_input}'
             ${single_paired.check_names}
         #elif $single_paired.single_paired_selector == "collection":
             #if $single_paired.input_pair.forward.is_of_type( 'fastq' ):
@@ -39,12 +39,12 @@
             "${single_paired.input_pair.forward}" "${single_paired.input_pair.reverse}"
             ${single_paired.check_names}
         #else:
-            #if $input_sequences.is_of_type( 'fastq' ):
+            #if $single_paired.input_sequences.is_of_type('fastq')
                 --fastq-input
             #else:
                 --fasta-input
             #end if
-            "$input_sequences"
+            '${single_paired.input_sequences}'
         #end if
 
         #if $split_reads:
@@ -79,7 +79,7 @@
 
         </conditional>
         <param label="Output classified and unclassified reads?" name="split_reads" type="boolean" help="Sets --unclassified-out and --classified-out"/>
-  
+
         <conditional name="quick_operation">
             <param name="quick" type="select" label="Enable quick operation?" help="--quick; Rather than searching all k-mers in a sequence, stop classification after a specified number of database hit">
                 <option value="yes">Yes</option>
@@ -127,23 +127,6 @@ Kraken is a taxonomic sequence classifier that assigns taxonomic labels to short
 
 -----
 
-**Kraken options**
-
-The Galaxy version of Kraken implements the following options::
-
-        
-  --fasta-input             Input is FASTA format
-  --fastq-input             Input is FASTQ format
-  --quick                   Quick operation (use first hit or hits)
-  --min-hits NUM            In quick op., number of hits req'd for classification
-                            NOTE: this is ignored if --quick is not specified
-  --unclassified-out        Print unclassified sequences to filename
-  --classified-out          Print classified sequences to filename
-
-  --only-classified-output  Print no Kraken output for unclassified sequences
-                          
-------
-
 **Output Format**
 
 Each sequence classified by Kraken results in a single line of output. Output lines contain five tab-delimited fields; from left to right, they are::
diff --git a/tool_collections/kraken/kraken_report/kraken-mpa-report.xml b/tool_collections/kraken/kraken_report/kraken-mpa-report.xml
index 2a2a57b8413..b821629bb2b 100644
--- a/tool_collections/kraken/kraken_report/kraken-mpa-report.xml
+++ b/tool_collections/kraken/kraken_report/kraken-mpa-report.xml
@@ -65,11 +65,7 @@ ${header_line}
 
 **What is Does**
 
-Kraken-mpa-report summarizes read counts across taxonomic ranks for multiple samples. This is convenient for comparing results across multiple experiments, conditions, locations, etc. It supports the following parameters::
-
---show-zeros    Display taxa even if they lack a read in any sample
---header-line   Display a header line indicating sample IDs
-                (sample IDs are the filenames)
+Kraken-mpa-report summarizes read counts across taxonomic ranks for multiple samples. This is convenient for comparing results across multiple experiments, conditions, locations, etc.
 
 -----
 

From 990f27c463c36c10a12d0c382d3b03a273ffcb0f Mon Sep 17 00:00:00 2001
From: Martin Cech <cech.marten@gmail.com>
Date: Fri, 17 Feb 2017 16:00:16 -0500
Subject: [PATCH 17/40] add more improvements by :eagle::eyes:

---
 tool_collections/kraken/macros.xml                          | 2 +-
 tool_collections/samtools/bam_to_cram/.shed.yml             | 2 +-
 .../samtools/bam_to_cram/samtools_bam_to_cram.xml           | 6 +++---
 .../samtools/samtools_bedcov/samtools_bedcov.xml            | 6 +++---
 4 files changed, 8 insertions(+), 8 deletions(-)

diff --git a/tool_collections/kraken/macros.xml b/tool_collections/kraken/macros.xml
index d851dd80594..37d8c429a72 100644
--- a/tool_collections/kraken/macros.xml
+++ b/tool_collections/kraken/macros.xml
@@ -16,7 +16,7 @@
     <xml name="input_database">
         <param label="Select a Kraken database" name="kraken_database" type="select">
             <options from_data_table="kraken_databases">
-                <validator message="No databases are available built-in" type="no_options" />
+                <validator message="No Kraken database is available" type="no_options" />
             </options>
         </param>
     </xml>
diff --git a/tool_collections/samtools/bam_to_cram/.shed.yml b/tool_collections/samtools/bam_to_cram/.shed.yml
index ba9dcb08cbb..06dc6400cce 100644
--- a/tool_collections/samtools/bam_to_cram/.shed.yml
+++ b/tool_collections/samtools/bam_to_cram/.shed.yml
@@ -5,7 +5,7 @@ description: Converts alignments in BAM format to CRAM format.
 long_description: |
   This tool uses the SAMtools toolkit to convert BAM to CRAM files. The CRAM
   format does additional compression relative to the reference genome which
-  makes the compression more effictient in terms of file size.
+  makes the compression more efficient in terms of file size.
 name: samtools_bam_to_cram
 owner: devteam
 homepage_url: https://samtools.github.io/
diff --git a/tool_collections/samtools/bam_to_cram/samtools_bam_to_cram.xml b/tool_collections/samtools/bam_to_cram/samtools_bam_to_cram.xml
index 91eb67e24b3..2d891b8c3d4 100644
--- a/tool_collections/samtools/bam_to_cram/samtools_bam_to_cram.xml
+++ b/tool_collections/samtools/bam_to_cram/samtools_bam_to_cram.xml
@@ -23,7 +23,7 @@
             #if $parameter_regions.target_region == "regions_bed_file"
                 -L "${parameter_regions.regions_bed_file}"
             #end if
-            
+
             -@\${GALAXY_SLOTS:-1}
             -C
             -T '${reference_source.input_reference}'
@@ -118,10 +118,10 @@
 
 **What this tool does**
 
-Converts alignments from the BAM format to the CRAM format using the SAMTools_ toolkit. The CRAM format does additional compression relative to the reference genome which makes the compression in terms of file size more effictient.
+Converts alignments from the BAM format to the CRAM format using the SAMTools_ toolkit. The CRAM format does additional compression relative to the reference genome which makes the compression in terms of file size more efficient.
 
 .. _SAMTools: http://samtools.sourceforge.net/samtools.shtml
     </help>
-    
+
     <expand macro="citations"></expand>
 </tool>
diff --git a/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml b/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml
index db09bd0ebd6..016c71c8b46 100644
--- a/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml
+++ b/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml
@@ -7,15 +7,15 @@
     <expand macro="stdio"></expand>
     <expand macro="version_command"></expand>
     <command><![CDATA[
-        for bamfile in 
+        for bamfile in
         #for dataset in $input_bams:
             "${dataset}"
         #end for
         ; do
             if [ ! -f \$bamfile.bai ] ; then
-                ln -s \$bamfile && samtools index `basename \$bamfile` ;
+                ln -s "\$bamfile" && samtools index "$(basename "\$bamfile")";
             else
-                ln -s \$bamfile && ln -s \$bamfile.bai ;
+                ln -s "\$bamfile" && ln -s "\$bamfile.bai";
             fi ;
         done ;
         samtools bedcov "${input_bed}"

From 9cfe78d19728f88fdbe7f4835a0cdfaaeb7609d4 Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Fri, 24 Feb 2017 09:26:05 +0100
Subject: [PATCH 18/40] typo breaking planemo test

---
 tool_collections/kraken/kraken/kraken.xml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/tool_collections/kraken/kraken/kraken.xml b/tool_collections/kraken/kraken/kraken.xml
index ee995b2e344..b0cf03071ba 100644
--- a/tool_collections/kraken/kraken/kraken.xml
+++ b/tool_collections/kraken/kraken/kraken.xml
@@ -22,7 +22,7 @@
 
         #end if
 
-        #if $single_paired.single_paired.single_paired_selector == 'yes'
+        #if $single_paired.single_paired_selector == 'yes'
             #if $forward_input.is_of_type( 'fastq' ):
                 --fastq-input
             #else:

From d0682b10328595fa53475af503937f648180cec1 Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Fri, 24 Feb 2017 11:24:41 +0100
Subject: [PATCH 19/40] samtools bedcov: proper usage of multiple bam files

---
 .../samtools_bedcov/samtools_bedcov.xml       | 26 ++++++++-----------
 1 file changed, 11 insertions(+), 15 deletions(-)

diff --git a/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml b/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml
index 016c71c8b46..feeb55e1a0b 100644
--- a/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml
+++ b/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml
@@ -7,22 +7,18 @@
     <expand macro="stdio"></expand>
     <expand macro="version_command"></expand>
     <command><![CDATA[
-        for bamfile in
-        #for dataset in $input_bams:
-            "${dataset}"
+        ## Ensure simlinks have unique filenames
+        #for $i, $bam in enumerate( $input_bams ):
+            ln -s -f '${bam}' '${i}.bam' &&
+            ln -s -f '${bam.metadata.bam_index}' '${i}.bam.bai' &&
         #end for
-        ; do
-            if [ ! -f \$bamfile.bai ] ; then
-                ln -s "\$bamfile" && samtools index "$(basename "\$bamfile")";
-            else
-                ln -s "\$bamfile" && ln -s "\$bamfile.bai";
-            fi ;
-        done ;
-        samtools bedcov "${input_bed}"
-        #for dataset in $input_bams:
-            `basename "${dataset}"`
-        #end for
-        > "${output}"
+        
+        samtools bedcov
+            '${input_bed}'
+            #for $i, $bam in enumerate( $input_bams ):
+                '${i}.bam'
+            #end for
+            > '${output}'
         ]]>
     </command>
     <inputs>

From 21b0a8f09ed9506a325851a38e432b415c5a8c58 Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Fri, 24 Feb 2017 12:26:16 +0100
Subject: [PATCH 20/40] updates test data of samtools flagstat and updates
 iterator in samtools bedcov

---
 .../samtools/samtools_bedcov/samtools_bedcov.xml            | 2 +-
 .../samtools_flagstat/test-data/samtools_flagstat_out1.txt  | 6 +++---
 2 files changed, 4 insertions(+), 4 deletions(-)

diff --git a/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml b/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml
index feeb55e1a0b..e97b54c2c07 100644
--- a/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml
+++ b/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml
@@ -15,7 +15,7 @@
         
         samtools bedcov
             '${input_bed}'
-            #for $i, $bam in enumerate( $input_bams ):
+            #for $i in range(len( $input_bams )):
                 '${i}.bam'
             #end for
             > '${output}'
diff --git a/tool_collections/samtools/samtools_flagstat/test-data/samtools_flagstat_out1.txt b/tool_collections/samtools/samtools_flagstat/test-data/samtools_flagstat_out1.txt
index 086fe6c2dd3..02f581e8960 100644
--- a/tool_collections/samtools/samtools_flagstat/test-data/samtools_flagstat_out1.txt
+++ b/tool_collections/samtools/samtools_flagstat/test-data/samtools_flagstat_out1.txt
@@ -2,12 +2,12 @@
 0 + 0 secondary
 0 + 0 supplementary
 0 + 0 duplicates
-25 + 0 mapped (12.50%:nan%)
+25 + 0 mapped (12.50%:-nan%)
 200 + 0 paired in sequencing
 100 + 0 read1
 100 + 0 read2
-0 + 0 properly paired (0.00%:nan%)
+0 + 0 properly paired (0.00%:-nan%)
 0 + 0 with itself and mate mapped
-25 + 0 singletons (12.50%:nan%)
+25 + 0 singletons (12.50%:-nan%)
 0 + 0 with mate mapped to a different chr
 0 + 0 with mate mapped to a different chr (mapQ>=5)

From ca8349674890bbb4fa7a1b8324552891766369ff Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Fri, 24 Feb 2017 17:48:06 +0100
Subject: [PATCH 21/40] textual changes

---
 .../kraken/kraken_filter/kraken-filter.xml    | 15 +--
 .../kraken_report/kraken-mpa-report.xml       | 58 ++++++------
 .../kraken/kraken_report/kraken-report.xml    | 16 ++--
 .../kraken_translate/kraken-translate.xml     | 21 +++--
 .../bam_to_cram/samtools_bam_to_cram.xml      | 10 +-
 .../samtools/bam_to_sam/bam_to_sam.xml        |  8 +-
 .../cram_to_bam/samtools_cram_to_bam.xml      | 14 ++-
 .../samtools/sam_to_bam/sam_to_bam.xml        | 39 +++++---
 .../samtools_bedcov/samtools_bedcov.xml       |  6 +-
 .../samtools_calmd/samtools_calmd.xml         | 16 ++--
 .../samtools_flagstat/samtools_flagstat.xml   | 51 +++++-----
 .../samtools_idxstats/samtools_idxstats.xml   | 24 ++---
 .../samtools_phase/samtools_phase.xml         | 19 ++--
 .../samtools_reheader/samtools_reheader.xml   | 19 ++--
 .../samtools_rmdup/samtools_rmdup.xml         | 93 ++++++++++---------
 .../samtools_slice_bam/samtools_slice_bam.xml | 36 +++----
 .../samtools/samtools_sort/samtools_sort.xml  | 24 +++--
 .../samtools_split/samtools_split.xml         |  6 +-
 .../samtools_stats/samtools_stats.xml         |  8 +-
 19 files changed, 259 insertions(+), 224 deletions(-)

diff --git a/tool_collections/kraken/kraken_filter/kraken-filter.xml b/tool_collections/kraken/kraken_filter/kraken-filter.xml
index 99206efcbb7..aaf8f31bf1d 100644
--- a/tool_collections/kraken/kraken_filter/kraken-filter.xml
+++ b/tool_collections/kraken/kraken_filter/kraken-filter.xml
@@ -8,15 +8,18 @@
     <expand macro="requirements" />
     <expand macro="stdio" />
     <expand macro="version_command" />
-    <command>
-        <![CDATA[
+    <command><![CDATA[
         @SET_DATABASE_PATH@ &&
-        kraken-filter @INPUT_DATABASE@ --threshold $threshold "${input}" > "$filtered_output"
-        ]]>
-    </command>
+        
+        kraken-filter
+            @INPUT_DATABASE@
+            --threshold $threshold '${input}' >
+            '$filtered_output'
+    ]]></command>
     <inputs>
         <param format="tabular" label="Kraken output" name="input" type="data" help="Select taxonomy classification produced by kraken"/>
-        <param label="Confidence threshold" max="1" min="0" name="threshold" type="float" value="0" help="--threshold; A number between 0 and 1; default=0"/>
+        <param max="1" min="0" name="threshold" type="float" value="0" label="Confidence threshold" help="A number between 0 and 1; default=0" argument="--threshold"/>
+        
         <expand macro="input_database" />
     </inputs>
     <outputs>
diff --git a/tool_collections/kraken/kraken_report/kraken-mpa-report.xml b/tool_collections/kraken/kraken_report/kraken-mpa-report.xml
index b821629bb2b..e12fb75a031 100644
--- a/tool_collections/kraken/kraken_report/kraken-mpa-report.xml
+++ b/tool_collections/kraken/kraken_report/kraken-mpa-report.xml
@@ -7,39 +7,39 @@
     <expand macro="requirements" />
     <expand macro="stdio" />
     <expand macro="version_command" />
-    <command>
-<![CDATA[
-
-#set $names = []
-
-#for $input_count, $input_classification in enumerate( $classification ):
-    #set $name_base = str( getattr( $input_classification, 'element_identifier', 'sample' ) ).replace( "/", '-' ).replace( "\t", "-" )
-    #set $name = $name_base
-    #set $i = 1
-    #while $name in $names:
-        #set $name = "%s_%s" % ( $name_base, $i )
-        #set $i = $i + 1
-    #end while
-    #silent $names.append( $name )
-    ln -s "${input_classification}" "${name}" &&
-#end for
+    <command><![CDATA[
+    #set $names = []
 
-@SET_DATABASE_PATH@ && kraken-mpa-report @INPUT_DATABASE@
+    #for $input_count, $input_classification in enumerate( $classification ):
+        #set $name_base = str( getattr( $input_classification, 'element_identifier', 'sample' ) ).replace( "/", '-' ).replace( "\t", "-" )
+        #set $name = $name_base
+        #set $i = 1
+        #while $name in $names:
+            #set $name = "%s_%s" % ( $name_base, $i )
+            #set $i = $i + 1
+        #end while
+        #silent $names.append( $name )
+        ln -s "${input_classification}" "${name}" &&
+    #end for
 
-#for $name in $names:
-    "${name}"
-#end for
+    @SET_DATABASE_PATH@ &&
+    
+    kraken-mpa-report
+        @INPUT_DATABASE@
+        #for $name in $names:
+            "${name}"
+        #end for
 
-${show_zeros}
-${header_line}
+        ${show_zeros}
+        ${header_line}
 
-> "$output_report"
-]]>
-    </command>
+        > '$output_report'
+    ]]></command>
     <inputs>
-        <param format="tabular" label="Kraken output" multiple="True" name="classification" type="data" />
-        <param checked="False" falsevalue="" help="--show-zeros" label="Display taxa even if they lack a read in any sample" name="show_zeros" truevalue="--show-zeros" type="boolean" />
-        <param checked="False" falsevalue="" help="--header-line" label="Display a header line indicating sample IDs" name="header_line" truevalue="--header-line" type="boolean" />
+        <param name="classification" format="tabular" label="Kraken output" multiple="True" type="data" />
+        <param name="show_zeros" checked="False" falsevalue="" help="--show-zeros" label="Display taxa even if they lack a read in any sample" truevalue="--show-zeros" type="boolean" />
+        <param name="header_line" checked="False" falsevalue="" help="--header-line" label="Display a header line indicating sample IDs" truevalue="--header-line" type="boolean" />
+
         <expand macro="input_database" />
     </inputs>
     <outputs>
@@ -71,7 +71,7 @@ Kraken-mpa-report summarizes read counts across taxonomic ranks for multiple sam
 
 **Output**
 
-The output of kraken-mpa-report is tab-delimited, with one line per taxon.
+The output of kraken-mpa-report is a tab-delimited table, with one line per taxon.
 
 ]]>
     </help>
diff --git a/tool_collections/kraken/kraken_report/kraken-report.xml b/tool_collections/kraken/kraken_report/kraken-report.xml
index 2883566e151..fb783f766cf 100644
--- a/tool_collections/kraken/kraken_report/kraken-report.xml
+++ b/tool_collections/kraken/kraken_report/kraken-report.xml
@@ -9,14 +9,14 @@
     <expand macro="requirements" />
     <expand macro="stdio" />
     <expand macro="version_command" />
-    <command>
-        <![CDATA[
+    <command><![CDATA[
         @SET_DATABASE_PATH@ &&
+        
         kraken-report @INPUT_DATABASE@ '${kraken_output}' > '$output_report'
-        ]]>
-    </command>
+    ]]></command>
     <inputs>
-        <param format="tabular" label="Kraken output" name="kraken_output" type="data" help="Select taxonomy classification produced by kraken"/>
+        <param name="kraken_output" format="tabular" label="Kraken output" type="data" help="Select taxonomy classification produced by kraken"/>
+        
         <expand macro="input_database" />
     </inputs>
     <outputs>
@@ -30,8 +30,7 @@
         </test>
     </tests>
 
-    <help>
-<![CDATA[
+    <help><![CDATA[
 
 .. class:: warningmark
 
@@ -52,7 +51,6 @@ The output of kraken-report is tab-delimited, with one line per taxon. The field
 
 The scientific names are indented using spaces, according to the tree
 structure specified by the taxonomy.
-    ]]>
-    </help>
+    ]]></help>
     <expand macro="citations" />
 </tool>
diff --git a/tool_collections/kraken/kraken_translate/kraken-translate.xml b/tool_collections/kraken/kraken_translate/kraken-translate.xml
index e5a96b8db70..9b0876d4751 100644
--- a/tool_collections/kraken/kraken_translate/kraken-translate.xml
+++ b/tool_collections/kraken/kraken_translate/kraken-translate.xml
@@ -8,15 +8,18 @@
     <expand macro="requirements" />
     <expand macro="stdio" />
     <expand macro="version_command" />
-    <command>
-        <![CDATA[
+    <command><![CDATA[
         @SET_DATABASE_PATH@ &&
-        kraken-translate @INPUT_DATABASE@ $mpa_format "${input}" > "${translated}"
-        ]]>
-    </command>
+        
+        kraken-translate
+            @INPUT_DATABASE@
+            $mpa_format
+            '${input}' >
+            '${translated}'
+    ]]></command>
     <inputs>
         <param format="tabular" label="Kraken output" name="input" type="data" help="Select taxonomy classification produced by kraken"/>
-        <param label="Restrict labels to standard rank assignments" name="mpa_format" truevalue="--mpa-format" falsevalue="" type="boolean" />
+        <param name="mpa_format" truevalue="--mpa-format" falsevalue="" type="boolean" label="Restrict labels to standard rank assignments" />
         <expand macro="input_database" />
     </inputs>
     <outputs>
@@ -30,8 +33,7 @@
             <output name="translated" file="kraken_translate_test1_output.tab" ftype="tabular"/>
         </test>
     </tests>
-    <help>
-        <![CDATA[
+    <help><![CDATA[
 
 .. class:: warningmark
 
@@ -54,7 +56,6 @@ Alternatively, kraken-translate accepts the option ``--mpa-format`` which will r
  SEQ1  d__Bacteria|p__Proteobacteria|c__Gammaproteobacteria|o__Enterobacteriales|f__Enterobacteriaceae|g__Escherichia|s__Escherichia_coli
 
 Taxonomy assignments above the superkingdom rank are represented as just "root" when using the ``--mpa-report`` option with kraken-translate.
-        ]]>
-    </help>
+    ]]></help>
     <expand macro="citations" />
 </tool>
diff --git a/tool_collections/samtools/bam_to_cram/samtools_bam_to_cram.xml b/tool_collections/samtools/bam_to_cram/samtools_bam_to_cram.xml
index 2d891b8c3d4..bd58c0cccee 100644
--- a/tool_collections/samtools/bam_to_cram/samtools_bam_to_cram.xml
+++ b/tool_collections/samtools/bam_to_cram/samtools_bam_to_cram.xml
@@ -4,9 +4,9 @@
     <macros>
         <import>macros.xml</import>
     </macros>
-    <expand macro="requirements"></expand>
-    <expand macro="stdio"></expand>
-    <expand macro="version_command"></expand>
+    <expand macro="requirements"/>
+    <expand macro="stdio"/>
+    <expand macro="version_command"/>
 
     <command>
     <![CDATA[
@@ -21,7 +21,7 @@
 
         samtools view
             #if $parameter_regions.target_region == "regions_bed_file"
-                -L "${parameter_regions.regions_bed_file}"
+                -L '${parameter_regions.regions_bed_file}'
             #end if
 
             -@\${GALAXY_SLOTS:-1}
@@ -123,5 +123,5 @@ Converts alignments from the BAM format to the CRAM format using the SAMTools_ t
 .. _SAMTools: http://samtools.sourceforge.net/samtools.shtml
     </help>
 
-    <expand macro="citations"></expand>
+    <expand macro="citations" />
 </tool>
diff --git a/tool_collections/samtools/bam_to_sam/bam_to_sam.xml b/tool_collections/samtools/bam_to_sam/bam_to_sam.xml
index ed53bad7cfb..66ee228a974 100644
--- a/tool_collections/samtools/bam_to_sam/bam_to_sam.xml
+++ b/tool_collections/samtools/bam_to_sam/bam_to_sam.xml
@@ -4,9 +4,9 @@
   <macros>
     <import>macros.xml</import>
   </macros>
-  <expand macro="requirements"></expand>
-  <expand macro="stdio"></expand>
-  <expand macro="version_command"></expand>
+  <expand macro="requirements"/>
+  <expand macro="stdio"/>
+  <expand macro="version_command"/>
 
   <command><![CDATA[
     samtools view -o '${output1}' ${header} '${input1}'
@@ -52,5 +52,5 @@ Converts BAM dataset to SAM using ``samtools view`` command::
 
 ]]>
   </help>
-  <expand macro="citations"></expand>
+  <expand macro="citations"/>
 </tool>
diff --git a/tool_collections/samtools/cram_to_bam/samtools_cram_to_bam.xml b/tool_collections/samtools/cram_to_bam/samtools_cram_to_bam.xml
index 16ad57a4e65..a0080224029 100644
--- a/tool_collections/samtools/cram_to_bam/samtools_cram_to_bam.xml
+++ b/tool_collections/samtools/cram_to_bam/samtools_cram_to_bam.xml
@@ -4,12 +4,11 @@
     <macros>
         <import>macros.xml</import>
     </macros>
-    <expand macro="requirements"></expand>
-    <expand macro="version_command"></expand>
-    <expand macro="stdio"></expand>
+    <expand macro="requirements"/>
+    <expand macro="version_command"/>
+    <expand macro="stdio"/>
 
-    <command>
-    <![CDATA[
+    <command><![CDATA[
         #if str( $input_alignment.metadata.cram_index ) != "None":
             ln
                 -f
@@ -33,8 +32,7 @@
             #if $parameter_regions.target_region == "region"
                 "${parameter_regions.region_string}"
             #end if
-    ]]>
-    </command>
+    ]]></command>
 
     <inputs>
         <param name="input_alignment" type="data" format="cram" label="CRAM alignment file"/>
@@ -112,5 +110,5 @@ Converts alignments from the CRAM format to the BAM format using the SAMTools_ t
 .. _SAMTools: http://samtools.sourceforge.net/samtools.shtml
     </help>
 
-    <expand macro="citations"></expand>
+    <expand macro="citations"/>
 </tool>
diff --git a/tool_collections/samtools/sam_to_bam/sam_to_bam.xml b/tool_collections/samtools/sam_to_bam/sam_to_bam.xml
index 183ce2fa66a..dd8e0a65598 100644
--- a/tool_collections/samtools/sam_to_bam/sam_to_bam.xml
+++ b/tool_collections/samtools/sam_to_bam/sam_to_bam.xml
@@ -1,13 +1,14 @@
 <tool id="sam_to_bam" name="SAM-to-BAM" version="2.1">
-  <description>convert SAM to BAM</description>
-  <macros>
-    <import>macros.xml</import>
-  </macros>
-  <expand macro="requirements"></expand>
-  <expand macro="stdio"></expand>
-  <expand macro="version_command"></expand>
-  <command>
-    <![CDATA[
+    <description>convert SAM to BAM</description>
+
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"/>
+    <expand macro="stdio"/>
+    <expand macro="version_command"/>
+
+    <command><![CDATA[
         #if $source.index_source == "history":
           ln -s '${source.ref_file}' input.fa &&
           samtools faidx input.fa &&
@@ -15,9 +16,19 @@
           ln -s '${source.index.fields.path}' input.fa &&
           ln -s '${source.index.fields.path}.fai' input.fa.fai &&
         #end if
-        samtools view -b -@ \${GALAXY_SLOTS:-1} -t input.fa.fai '$input1' | samtools sort -O bam -@ \${GALAXY_SLOTS:-1} -o '$output1' -T temp
-    ]]>
-    </command>
+
+        samtools view
+            -b
+            -@ \${GALAXY_SLOTS:-1}
+            -t input.fa.fai '$input1' |
+    
+        samtools sort
+            -O bam
+            -@ \${GALAXY_SLOTS:-1}
+            -o '$output1'
+            -T temp
+    ]]></command>
+
     <inputs>
         <conditional name="source">
             <param label="Choose the source for the reference genome" name="index_source" type="select">
@@ -43,7 +54,7 @@
         </conditional>
     </inputs>
     <outputs>
-        <data format="bam" label="${tool.name} on ${on_string}: converted BAM" name="output1">
+        <data name="output1" format="bam" label="${tool.name} on ${on_string}: converted BAM">
             <actions>
                 <conditional name="source.index_source">
                     <when value="cached">
@@ -85,5 +96,5 @@ Converts SAM dataset into its binary, BAM, representation using ``samtools sort`
 
 ]]>
   </help>
-  <expand macro="citations"></expand>
+  <expand macro="citations"/>
 </tool>
diff --git a/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml b/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml
index e97b54c2c07..86a5fca3314 100644
--- a/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml
+++ b/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml
@@ -3,9 +3,9 @@
   <macros>
     <import>macros.xml</import>
   </macros>
-  <expand macro="requirements"></expand>
-    <expand macro="stdio"></expand>
-    <expand macro="version_command"></expand>
+  <expand macro="requirements"/>
+    <expand macro="stdio"/>
+    <expand macro="version_command"/>
     <command><![CDATA[
         ## Ensure simlinks have unique filenames
         #for $i, $bam in enumerate( $input_bams ):
diff --git a/tool_collections/samtools/samtools_calmd/samtools_calmd.xml b/tool_collections/samtools/samtools_calmd/samtools_calmd.xml
index e0c7d0243c1..62ecc4b2ff7 100644
--- a/tool_collections/samtools/samtools_calmd/samtools_calmd.xml
+++ b/tool_collections/samtools/samtools_calmd/samtools_calmd.xml
@@ -1,11 +1,11 @@
 <tool id="samtools_calmd" name="CalMD" version="2.0">
-  <description>recalculate MD/NM tags</description>
-  <macros>
-    <import>macros.xml</import>
-  </macros>
-  <expand macro="requirements"></expand>
-  <expand macro="stdio"></expand>
-  <expand macro="version_command"></expand>
+    <description>recalculate MD/NM tags</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"/>
+    <expand macro="stdio"/>
+    <expand macro="version_command"/>
     <command><![CDATA[
         #if str( $reference_source.reference_source_selector ) == "history":
             #set ref_fa = 'ref.fa'
@@ -95,5 +95,5 @@ From SAM format specification::
 See refernces for more information about SAM format tags.
 
     </help>
-    <expand macro="citations"></expand>
+    <expand macro="citations"/>
 </tool>
diff --git a/tool_collections/samtools/samtools_flagstat/samtools_flagstat.xml b/tool_collections/samtools/samtools_flagstat/samtools_flagstat.xml
index 1d53cec9a52..533ce3cdb18 100644
--- a/tool_collections/samtools/samtools_flagstat/samtools_flagstat.xml
+++ b/tool_collections/samtools/samtools_flagstat/samtools_flagstat.xml
@@ -1,26 +1,33 @@
 <tool id="samtools_flagstat" name="Flagstat" version="2.0">
-  <description>tabulate descriptive stats for BAM datset</description>
-  <macros>
-    <import>macros.xml</import>
-  </macros>
-  <expand macro="requirements"></expand>
-  <expand macro="stdio"></expand>
-  <expand macro="version_command"></expand>
-  <command>samtools flagstat "$input1" > "$output1"
-  </command>
-  <inputs>
-    <param name="input1" type="data" format="bam" label="BAM File to Convert" />
-  </inputs>
-  <outputs>
+    <description>tabulate descriptive stats for BAM datset</description>
+
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"/>
+    <expand macro="stdio"/>
+    <expand macro="version_command"/>
+
+    <command><![CDATA[
+        samtools flagstat '$input1' > '$output1'
+    ]]></command>
+
+    <inputs>
+        <param name="input1" type="data" format="bam" label="BAM File to report statistics of" />
+    </inputs>
+
+    <outputs>
     <data name="output1" format="txt" />
-  </outputs>
-  <tests>
-    <test>
-      <param name="input1" value="samtools_flagstat_input1.bam" ftype="bam" />
-      <output name="output1" file="samtools_flagstat_out1.txt" />
-    </test>
-  </tests>
-  <help>
+    </outputs>
+
+    <tests>
+        <test>
+          <param name="input1" value="samtools_flagstat_input1.bam" ftype="bam" />
+
+          <output name="output1" file="samtools_flagstat_out1.txt" />
+        </test>
+    </tests>
+    <help>
 
 **What it does**
 
@@ -41,5 +48,5 @@ Uses ``samtools flagstat`` command to print descriptive information for a BAM da
   0 + 0 with mate mapped to a different chr (mapQ>=5)
   
   </help>
-  <expand macro="citations"></expand>
+  <expand macro="citations"/>
 </tool>
diff --git a/tool_collections/samtools/samtools_idxstats/samtools_idxstats.xml b/tool_collections/samtools/samtools_idxstats/samtools_idxstats.xml
index 57bdb584a2d..2473539d797 100644
--- a/tool_collections/samtools/samtools_idxstats/samtools_idxstats.xml
+++ b/tool_collections/samtools/samtools_idxstats/samtools_idxstats.xml
@@ -3,19 +3,14 @@
     <macros>
       <import>macros.xml</import>
     </macros>
-    <expand macro="requirements"></expand>
-    <expand macro="stdio"></expand>
-    <expand macro="version_command"></expand>
+    <expand macro="requirements"/>
+    <expand macro="stdio"/>
+    <expand macro="version_command"/>
     <command><![CDATA[
-if [ ! -f "${input}.bai" ] ; then
-    ln -s "${input}" input.bam ;
-    samtools sort -o sorted.bam -O bam -T sts "${input}" ;
-    samtools index sorted.bam ;
-    samtools idxstats sorted.bam > "${output}" ;
-else
-    samtools idxstats "${input}" > "${output}" ;
-fi]]>
-    </command>
+        ln -f -s '$input.metadata.bam_index' '\$(basename $input).bai' &&
+        
+        samtools idxstats '${input}' > '${output}'
+    ]]></command>
     <inputs>
         <param name="input" type="data" format="bam" label="BAM file" />
     </inputs>
@@ -28,8 +23,7 @@ fi]]>
             <output name="output" file="samtools_idxstats_out.tabular" compare="diff" ftype="tabular" />
         </test>
     </tests>
-    <help>
-<![CDATA[
+    <help><![CDATA[
 **What it does**
 
 Runs the ``samtools idxstats`` command. It retrieves and prints stats in the index file.
@@ -68,5 +62,5 @@ In this BAM file, the final column was otherwise zero.
 Peter J.A. Cock (2013), `Galaxy wrapper <https://github.com/peterjc/pico_galaxy/tree/master/tools/samtools_idxstats>`_ for the samtools idxstats command
 ]]>
     </help>
-    <expand macro="citations"></expand>
+    <expand macro="citations"/>
 </tool>
diff --git a/tool_collections/samtools/samtools_phase/samtools_phase.xml b/tool_collections/samtools/samtools_phase/samtools_phase.xml
index eb89119e03c..d63644659fe 100644
--- a/tool_collections/samtools/samtools_phase/samtools_phase.xml
+++ b/tool_collections/samtools/samtools_phase/samtools_phase.xml
@@ -1,12 +1,13 @@
 <tool id="samtools_phase" name="Call and phase" version="2.0">
     <description>heterozygous SNPs</description>
     <macros>
-    <import>macros.xml</import>
-  </macros>
-  <expand macro="requirements"></expand>
-  <expand macro="stdio"></expand>
-  <expand macro="version_command"></expand>
-    <command>samtools phase -b "phase_wrapper"
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"/>
+    <expand macro="stdio"/>
+    <expand macro="version_command"/>
+    <command><![CDATA[
+    samtools phase -b "phase_wrapper"
         #if str($option_set.option_sets) == 'advanced':
             ${option_set.ignore_chimeras}
             -k $option_set.block_length
@@ -17,8 +18,8 @@
         #else
             -k 13 -q 37 -Q 13 -D 256
         #end if
-        "$input_bam" &gt; "$phase_sets"
-    </command>
+        '$input_bam' > '$phase_sets'
+    ]]></command>
     <inputs>
         <param format="bam" name="input_bam" type="data" label="Select dataset to phase"/>
         <conditional name="option_set">
@@ -126,5 +127,5 @@ Call and phase heterozygous SNPs
      - *off*
 
     </help>
-    <expand macro="citations"></expand>
+    <expand macro="citations"/>
 </tool>
diff --git a/tool_collections/samtools/samtools_reheader/samtools_reheader.xml b/tool_collections/samtools/samtools_reheader/samtools_reheader.xml
index ac8959dd371..feb2417a234 100644
--- a/tool_collections/samtools/samtools_reheader/samtools_reheader.xml
+++ b/tool_collections/samtools/samtools_reheader/samtools_reheader.xml
@@ -1,12 +1,17 @@
 <tool id="samtools_reheader" name="Reheader" version="2.0">
     <description>copy SAM/BAM header between datasets</description>
     <macros>
-    <import>macros.xml</import>
-  </macros>
-  <expand macro="requirements"></expand>
-  <expand macro="stdio"></expand>
-  <expand macro="version_command"></expand>
-    <command><![CDATA[samtools reheader "${input_header}" "${input_file}" > "${output}"]]></command>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"/>
+    <expand macro="stdio"/>
+    <expand macro="version_command"/>
+    <command><![CDATA[
+        samtools reheader
+            '${input_header}'
+            '${input_file}' >
+            '${output}'
+    ]]></command>
     <inputs>
         <param name="input_header" type="data" format="sam,bam" label="Select source dataset" help="Header from this dataset will be used to replace header in the target dataset. May be either SAM or BAM dataset."/>
         <param name="input_file" type="data" format="bam" label="Select target dataset" help="Header from the source dataset will be inserted here. Should be a BAM dataset."/>
@@ -27,6 +32,6 @@
 Copies header from *source* dataset into *target* dataset using ``samtools reheader`` command.
 
     </help>
-    <expand macro="citations"></expand>
+    <expand macro="citations"/>
 </tool>
 
diff --git a/tool_collections/samtools/samtools_rmdup/samtools_rmdup.xml b/tool_collections/samtools/samtools_rmdup/samtools_rmdup.xml
index dae1fce3976..a8ebc2755bb 100644
--- a/tool_collections/samtools/samtools_rmdup/samtools_rmdup.xml
+++ b/tool_collections/samtools/samtools_rmdup/samtools_rmdup.xml
@@ -1,48 +1,55 @@
 <tool id="samtools_rmdup" name="RmDup" version="2.0">
-  <description>remove PCR duplicates</description>
-  <macros>
-    <import>macros.xml</import>
-  </macros>
-  <requirements>
-    <requirement type="package" version="0.1.19">samtools</requirement>
-  </requirements>
-  <expand macro="stdio"></expand>
-  <expand macro="version_command"></expand>
-  <command>samtools rmdup 
-  #if str( $bam_paired_end_type.bam_paired_end_type_selector ) == "PE"
-      ${bam_paired_end_type.force_se}
-  #else:
-      -s
-  #end if
-  "$input1" "$output1"
-  </command>
-  <inputs>
-    <param name="input1" type="data" format="bam" label="BAM File" />
+    <description>remove PCR duplicates</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <requirements>
+        <requirement type="package" version="0.1.19">samtools</requirement>
+    </requirements>
     
-    <conditional name="bam_paired_end_type">
-      <param name="bam_paired_end_type_selector" type="select" label="Is this paired-end or single end data">
-        <option value="PE" selected="True">BAM is paired-end</option>
-        <option value="SE">BAM is single-end (-s)</option>
-      </param>
-      <when value="PE">
-        <param name="force_se" type="boolean" label="Treat as single-end" help="-S" truevalue="-S" falsevalue="" checked="False"/>
-      </when>
-      <when value="SE" /> <!-- No extra parameters here -->
-    </conditional>
+    <expand macro="stdio"/>
+    <expand macro="version_command"/>
     
-  </inputs>
-  <outputs>
-    <data name="output1" format="bam" />
-  </outputs>
-  <tests>
-    <test>
-      <param name="input1" value="samtools-rmdup-input1.bam" ftype="bam" />
-      <param name="bam_paired_end_type_selector" value="PE" />
-      <param name="force_se" />
-      <output name="output1" file="samtools-rmdup-test1.bam" ftype="bam" sort="True" />
-    </test>
-  </tests>
-  <help>
+    <command><![CDATA[
+    samtools rmdup 
+        #if str( $bam_paired_end_type.bam_paired_end_type_selector ) == "PE"
+            ${bam_paired_end_type.force_se}
+        #else:
+            -s
+        #end if
+        '$input1'
+        '$output1'
+    ]]></command>
+    
+    <inputs>
+        <param name="input1" type="data" format="bam" label="BAM File" />
+
+        <conditional name="bam_paired_end_type">
+            <param name="bam_paired_end_type_selector" type="select" label="Is this paired-end or single end data">
+                <option value="PE" selected="True">BAM is paired-end</option>
+                <option value="SE">BAM is single-end (-s)</option>
+            </param>
+            <when value="PE">
+                <param name="force_se" type="boolean" label="Treat as single-end" help="-S" truevalue="-S" falsevalue="" checked="False"/>
+            </when>
+            <when value="SE" /> <!-- No extra parameters here -->
+        </conditional>
+    </inputs>
+
+    <outputs>
+        <data name="output1" format="bam" />
+    </outputs>
+
+    <tests>
+        <test>
+            <param name="input1" value="samtools-rmdup-input1.bam" ftype="bam" />
+            <param name="bam_paired_end_type_selector" value="PE" />
+            <param name="force_se" />
+
+            <output name="output1" file="samtools-rmdup-test1.bam" ftype="bam" sort="True" />
+        </test>
+    </tests>
+    <help>
 
 **What it does**
 
@@ -52,5 +59,5 @@ Remove potential PCR duplicates: if multiple read pairs have identical external
   -S    treat PE reads as SE in rmdup (force -s)
 
   </help>
-    <expand macro="citations"></expand>
+    <expand macro="citations"/>
 </tool>
diff --git a/tool_collections/samtools/samtools_slice_bam/samtools_slice_bam.xml b/tool_collections/samtools/samtools_slice_bam/samtools_slice_bam.xml
index c95c19b74c8..e9292c4215b 100644
--- a/tool_collections/samtools/samtools_slice_bam/samtools_slice_bam.xml
+++ b/tool_collections/samtools/samtools_slice_bam/samtools_slice_bam.xml
@@ -1,16 +1,16 @@
 <tool id="samtools_slice_bam" name="Slice" version="2.0">
-  <description>BAM by genomic regions</description>
-  <macros>
-    <import>macros.xml</import>
-  </macros>
-  <!-- <code file="samtools_slice_options.py"/> -->
-  <expand macro="requirements"></expand>
-  <expand macro="stdio"></expand>
-  <expand macro="version_command"></expand>
-    <command>
-<![CDATA[
-    ln -s "${input_bam}" temp_input.bam &&
-    ln -s "${input_bam.metadata.bam_index}" temp_input.bam.bai &&
+    <description>BAM by genomic regions</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+
+    <expand macro="requirements"/>
+    <expand macro="stdio"/>
+    <expand macro="version_command"/>
+
+    <command><![CDATA[
+    ln -s '${input_bam}' temp_input.bam &&
+    ln -s '${input_bam.metadata.bam_index}' temp_input.bam.bai &&
 
     #if str($slice_method.slice_method_selector) == "bed":
     
@@ -33,9 +33,13 @@
 
     #end if
 
-    samtools sort -O bam -T sorted -@ \${GALAXY_SLOTS:-1} -o "${output_bam}" unsorted_output.bam
-]]>
-    </command>
+    samtools sort
+        -O bam
+        -T sorted
+        -@ \${GALAXY_SLOTS:-1}
+        -o '${output_bam}'
+        unsorted_output.bam
+    ]]></command>
     <inputs>
         <param name="input_bam" format="bam" label="Select BAM dataset to slice" type="data" />
         <conditional name="slice_method">
@@ -119,5 +123,5 @@ This tool is based on ``samtools view`` command.
 
 ]]>
   </help>
-    <expand macro="citations"></expand>
+    <expand macro="citations"/>
 </tool>
diff --git a/tool_collections/samtools/samtools_sort/samtools_sort.xml b/tool_collections/samtools/samtools_sort/samtools_sort.xml
index 96b2ad857db..80c17ef2203 100644
--- a/tool_collections/samtools/samtools_sort/samtools_sort.xml
+++ b/tool_collections/samtools/samtools_sort/samtools_sort.xml
@@ -1,12 +1,20 @@
 <tool id="samtools_sort" name="Sort" version="2.0">
     <description>BAM dataset</description>
     <macros>
-    <import>macros.xml</import>
-  </macros>
-  <expand macro="requirements"></expand>
-    <expand macro="stdio"></expand>
-    <expand macro="version_command"></expand>
-    <command>samtools sort $sort_mode -@ \${GALAXY_SLOTS:-1} -o "${output1}" -O bam -T dataset "${input1}"</command>
+      <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"/>
+    <expand macro="stdio"/>
+    <expand macro="version_command"/>
+    <command><![CDATA[
+        samtools sort
+            $sort_mode
+            -@ \${GALAXY_SLOTS:-1}
+            -o '${output1}'
+            -O bam
+            -T dataset
+            '${input1}'
+    ]]></command>
     <inputs>
         <param name="input1" type="data" format="bam" label="BAM File" />
         <param name="sort_mode" type="select" label="Sort by ">
@@ -33,8 +41,6 @@
 **What it does**
 
 This tool uses ``samtools sort`` command to sort BAM datasets in coordinate or read name order.
-
-
     </help>
-    <expand macro="citations"></expand>
+    <expand macro="citations"/>
 </tool>
diff --git a/tool_collections/samtools/samtools_split/samtools_split.xml b/tool_collections/samtools/samtools_split/samtools_split.xml
index bfd2e884970..293fab0efa6 100644
--- a/tool_collections/samtools/samtools_split/samtools_split.xml
+++ b/tool_collections/samtools/samtools_split/samtools_split.xml
@@ -3,9 +3,9 @@
     <macros>
         <import>macros.xml</import>
     </macros>
-    <expand macro="requirements"></expand>
-    <expand macro="stdio"></expand>
-    <expand macro="version_command"></expand>
+    <expand macro="requirements"/>
+    <expand macro="stdio"/>
+    <expand macro="version_command"/>
     <command><![CDATA[
         samtools split -f 'Read_Group_%!.bam'
         #if $header:
diff --git a/tool_collections/samtools/samtools_stats/samtools_stats.xml b/tool_collections/samtools/samtools_stats/samtools_stats.xml
index 83408f663d8..4b75ac06966 100644
--- a/tool_collections/samtools/samtools_stats/samtools_stats.xml
+++ b/tool_collections/samtools/samtools_stats/samtools_stats.xml
@@ -3,9 +3,9 @@
     <macros>
     <import>macros.xml</import>
   </macros>
-    <expand macro="requirements"></expand>
-    <expand macro="stdio"></expand>
-    <expand macro="version_command"></expand>
+    <expand macro="requirements"/>
+    <expand macro="stdio"/>
+    <expand macro="version_command"/>
     <command><![CDATA[
         #if $use_reference.use_ref_selector == "yes":
             #if $use_reference.reference_source.reference_source_selector == "history":
@@ -289,6 +289,6 @@ This tool runs the ``samtools stats`` command. It has the following options::
                                         will provide options for selecting a reference cached as this Galaxy instance or choosing
                                         one from history.
     ]]></help>
-    <expand macro="citations"></expand>
+    <expand macro="citations"/>
 </tool>
 

From 8a279e52f8ad65e57639f8cea2345c881beae0ad Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Fri, 24 Feb 2017 21:46:12 +0100
Subject: [PATCH 22/40] kraken: more textual changes

---
 tool_collections/kraken/kraken/kraken.xml     | 109 +++++++++---------
 .../kraken/kraken_filter/kraken-filter.xml    |  16 +--
 .../kraken_report/kraken-mpa-report.xml       |  14 ++-
 .../kraken/kraken_report/kraken-report.xml    |  18 +--
 .../kraken_translate/kraken-translate.xml     |  16 +--
 tool_collections/kraken/macros.xml            |   9 +-
 6 files changed, 92 insertions(+), 90 deletions(-)

diff --git a/tool_collections/kraken/kraken/kraken.xml b/tool_collections/kraken/kraken/kraken.xml
index b0cf03071ba..660ef89c1cf 100644
--- a/tool_collections/kraken/kraken/kraken.xml
+++ b/tool_collections/kraken/kraken/kraken.xml
@@ -7,56 +7,56 @@
         <import>macros.xml</import>
     </macros>
     <expand macro="requirements" />
-    <expand macro="stdio" />
     <expand macro="version_command" />
-    <command>
+    <command detect_errors="exit_code">
         <![CDATA[
         @SET_DATABASE_PATH@ &&
-        kraken --threads \${GALAXY_SLOTS:-1} @INPUT_DATABASE@
+        
+        kraken
+            --threads \${GALAXY_SLOTS:-1}
+            @INPUT_DATABASE@
+            ${only_classified_output}
 
-        ${only_classified_output}
+            #if str( $quick_operation.quick ) == "yes":
+                --quick
+                --min-hits ${quick_operation.min_hits}
 
-        #if str( $quick_operation.quick ) == "yes":
-            --quick
-            --min-hits ${quick_operation.min_hits}
-
-        #end if
-
-        #if $single_paired.single_paired_selector == 'yes'
-            #if $forward_input.is_of_type( 'fastq' ):
-                --fastq-input
-            #else:
-                --fasta-input
             #end if
-            '${single_paired.forward_input}' '${single_paired.reverse_input}'
-            ${single_paired.check_names}
-        #elif $single_paired.single_paired_selector == "collection":
-            #if $single_paired.input_pair.forward.is_of_type( 'fastq' ):
-                --fastq-input
-            #else:
-                --fasta-input
-            #end if
-            "${single_paired.input_pair.forward}" "${single_paired.input_pair.reverse}"
-            ${single_paired.check_names}
-        #else:
-            #if $single_paired.input_sequences.is_of_type('fastq')
-                --fastq-input
+
+            #if $single_paired.single_paired_selector == 'yes'
+                #if $forward_input.is_of_type( 'fastq' ):
+                    --fastq-input
+                #else:
+                    --fasta-input
+                #end if
+                '${single_paired.forward_input}' '${single_paired.reverse_input}'
+                ${single_paired.check_names}
+            #elif $single_paired.single_paired_selector == "collection":
+                #if $single_paired.input_pair.forward.is_of_type( 'fastq' ):
+                    --fastq-input
+                #else:
+                    --fasta-input
+                #end if
+                "${single_paired.input_pair.forward}" "${single_paired.input_pair.reverse}"
+                ${single_paired.check_names}
             #else:
-                --fasta-input
+                #if $single_paired.input_sequences.is_of_type('fastq')
+                    --fastq-input
+                #else:
+                    --fasta-input
+                #end if
+                '${single_paired.input_sequences}'
             #end if
-            '${single_paired.input_sequences}'
-        #end if
 
-        #if $split_reads:
-            --classified-out "${classified_out}" --unclassified-out "${unclassified_out}"
-        #end if
+            #if $split_reads:
+                --classified-out "${classified_out}" --unclassified-out "${unclassified_out}"
+            #end if
 
-        ## The --output option was changed to redirect as it does not work properly is some situations. For example, on test database the tool classifies 4 reads but does not write them into a file if --output is specified. It does however print correct output into STDOUT. This behavior can be re-created with test database provided in test-data/test_db/ folder. This is the reason for incrementing version number from 1.1.2 to 1.1.3
+            ## The --output option was changed to redirect as it does not work properly is some situations. For example, on test database the tool classifies 4 reads but does not write them into a file if --output is specified. It does however print correct output into STDOUT. This behavior can be re-created with test database provided in test-data/test_db/ folder. This is the reason for incrementing version number from 1.1.2 to 1.1.3
 
-        > "${output}"
-        ##kraken-translate --db ${kraken_database.fields.name} "${output}" > "${translated}"
-        ]]>
-    </command>
+            > "${output}"
+            ##kraken-translate --db ${kraken_database.fields.name} "${output}" > "${translated}"
+    ]]></command>
     <inputs>
         <conditional name="single_paired">
             <param name="single_paired_selector" type="select" label="Single or paired reads" help="--paired">
@@ -65,12 +65,12 @@
                 <option selected="True" value="no">Single</option>
             </param>
             <when value="collection">
-                <param format="fasta,fastq" label="Collection of paired reads" name="input_pair" type="data_collection" collection_type="paired" help="FASTA or FASTQ datasets" />
-                <param name="check_names" type="boolean" checked="False" truevalue="--paired --check-names" falsevalue="--paired" label="Verify read names match" help="--check-names" />
+                <param format="fasta,fastq" name="input_pair" type="data_collection" collection_type="paired" label="Collection of paired reads" help="FASTA or FASTQ datasets" />
+                <param name="check_names" argument="--check-names" type="boolean" checked="False" truevalue="--paired --check-names" falsevalue="--paired" label="Verify read names match"/>
             </when>
             <when value="yes">
-                <param format="fasta,fastq" label="Forward strand" name="forward_input" type="data" help="FASTA or FASTQ dataset"/>
-                <param format="fasta,fastq" label="Reverse strand" name="reverse_input" type="data" help="FASTA or FASTQ dataset"/>
+                <param format="fasta,fastq" name="forward_input" type="data" label="Forward strand" help="FASTA or FASTQ dataset"/>
+                <param format="fasta,fastq" name="reverse_input" type="data" label="Reverse strand" help="FASTA or FASTQ dataset"/>
                 <param name="check_names" type="boolean" checked="False" truevalue="--paired --check-names" falsevalue="--paired" label="Verify read names match" help="--check-names" />
             </when>
             <when value="no">
@@ -78,33 +78,35 @@
             </when>
 
         </conditional>
-        <param label="Output classified and unclassified reads?" name="split_reads" type="boolean" help="Sets --unclassified-out and --classified-out"/>
+        <param name="split_reads" type="boolean" label="Output classified and unclassified reads?" help="Sets --unclassified-out and --classified-out"/>
 
         <conditional name="quick_operation">
-            <param name="quick" type="select" label="Enable quick operation?" help="--quick; Rather than searching all k-mers in a sequence, stop classification after a specified number of database hit">
+            <param argument="--quick" type="select" label="Enable quick operation"
+                   help="Quick mode: rather than searching all k-mers in a sequence, stop classification after a specified number of database hit">
                 <option value="yes">Yes</option>
                 <option selected="True" value="no">No</option>
             </param>
             <when value="yes">
-                <param name="min_hits" type="integer" value="1" label="Number of hits required for classification" help="--min-hits; min-hits will allow you to require multiple hits before declaring a sequence classified, which can be especially useful with custom databases when testing to see if sequences either do or do not belong to a particular genome; default=1"/>
-            </when>
-            <when value="no">
-                <!-- Do absolutely nothing -->
+                <param name="min_hits" argument="--min-hits" type="integer" value="1" label="Number of hits required for classification"
+                       help="min-hits will allow you to require multiple hits before declaring a sequence classified, which can be especially useful with custom databases when testing to see if sequences either do or do not belong to a particular genome; default=1"/>
             </when>
+            <when value="no"/><!-- Do absolutely nothing -->
         </conditional>
 
-        <param name="only_classified_output" type="boolean" checked="False" truevalue="--only-classified-output" falsevalue="" label="Print no Kraken output for unclassified sequences" help="--only-classified-output"/>
+        <param name="only_classified_output" argument="--only-classified-output" type="boolean" checked="False"
+               truevalue="--only-classified-output" falsevalue=""
+               label="Print no Kraken output for unclassified sequences"/>
 
         <expand macro="input_database" />
     </inputs>
     <outputs>
-        <data format_source="input_sequences" label="${tool.name} on ${on_string}: Classified reads" name="classified_out">
+        <data name="classified_out" format_source="input_sequences" label="${tool.name} on ${on_string}: Classified reads">
             <filter>(split_reads)</filter>
         </data>
-        <data format_source="input_sequences" label="${tool.name} on ${on_string}: Unclassified reads" name="unclassified_out">
+        <data name="unclassified_out" format_source="input_sequences" label="${tool.name} on ${on_string}: Unclassified reads">
             <filter>(split_reads)</filter>
         </data>
-        <data format="tabular" label="${tool.name} on ${on_string}: Classification" name="output" />
+        <data name="output" format="tabular" label="${tool.name} on ${on_string}: Classification"/>
         <!--<data format="tabular" label="${tool.name} on ${on_string}: Translated classification" name="translated" />-->
     </outputs>
 
@@ -116,6 +118,7 @@
             <param name="quick" value="no"/>
             <param name="only-classified-output" value="false"/>
             <param name="kraken_database" value="test_db"/>
+
             <output name="output" file="kraken_test1_output.tab" ftype="tabular"/>
         </test>
     </tests>
diff --git a/tool_collections/kraken/kraken_filter/kraken-filter.xml b/tool_collections/kraken/kraken_filter/kraken-filter.xml
index aaf8f31bf1d..8e1c6a13bb4 100644
--- a/tool_collections/kraken/kraken_filter/kraken-filter.xml
+++ b/tool_collections/kraken/kraken_filter/kraken-filter.xml
@@ -1,24 +1,23 @@
 <tool id="kraken-filter" name="Kraken-filter" version="1.2.1">
-    <description>
-        filter classification by confidence score
-    </description>
+    <description>filter classification by confidence score</description>
     <macros>
         <import>macros.xml</import>
     </macros>
     <expand macro="requirements" />
-    <expand macro="stdio" />
     <expand macro="version_command" />
-    <command><![CDATA[
+    <command detect_errors="exit_code"><![CDATA[
         @SET_DATABASE_PATH@ &&
         
         kraken-filter
             @INPUT_DATABASE@
-            --threshold $threshold '${input}' >
+            --threshold $threshold
+            '${input}' >
             '$filtered_output'
     ]]></command>
     <inputs>
-        <param format="tabular" label="Kraken output" name="input" type="data" help="Select taxonomy classification produced by kraken"/>
-        <param max="1" min="0" name="threshold" type="float" value="0" label="Confidence threshold" help="A number between 0 and 1; default=0" argument="--threshold"/>
+        <param name="input" type="data" format="tabular" label="Kraken output" help="Select taxonomy classification produced by kraken"/>
+        <param argument="--threshold" type="float" value="0" min="0" max="1"
+               label="Confidence threshold" help="A floating point number between 0 and 1; default=0"/>
         
         <expand macro="input_database" />
     </inputs>
@@ -30,6 +29,7 @@
             <param name="input" value="kraken_filter_test1.tab"/>
             <param name="threshold" value="0"/>
             <param name="kraken_database" value="test_db"/>
+
             <output name="output" file="kraken_filter_test1_output.tab" ftype="tabular"/>
         </test>
     </tests>
diff --git a/tool_collections/kraken/kraken_report/kraken-mpa-report.xml b/tool_collections/kraken/kraken_report/kraken-mpa-report.xml
index e12fb75a031..8b24450c4ef 100644
--- a/tool_collections/kraken/kraken_report/kraken-mpa-report.xml
+++ b/tool_collections/kraken/kraken_report/kraken-mpa-report.xml
@@ -5,9 +5,8 @@
         <import>macros.xml</import>
     </macros>
     <expand macro="requirements" />
-    <expand macro="stdio" />
     <expand macro="version_command" />
-    <command><![CDATA[
+    <command detect_errors="exit_code"><![CDATA[
     #set $names = []
 
     #for $input_count, $input_classification in enumerate( $classification ):
@@ -19,7 +18,7 @@
             #set $i = $i + 1
         #end while
         #silent $names.append( $name )
-        ln -s "${input_classification}" "${name}" &&
+        ln -s '${input_classification}' '${name}' &&
     #end for
 
     @SET_DATABASE_PATH@ &&
@@ -37,8 +36,10 @@
     ]]></command>
     <inputs>
         <param name="classification" format="tabular" label="Kraken output" multiple="True" type="data" />
-        <param name="show_zeros" checked="False" falsevalue="" help="--show-zeros" label="Display taxa even if they lack a read in any sample" truevalue="--show-zeros" type="boolean" />
-        <param name="header_line" checked="False" falsevalue="" help="--header-line" label="Display a header line indicating sample IDs" truevalue="--header-line" type="boolean" />
+        <param name="show_zeros" argument="--show-zeros" type="boolean" falsevalue=""  truevalue="--show-zeros" checked="False"
+               label="Display taxa even if they lack a read in any sample" />
+        <param name="header_line" argument="--header-line" type="boolean" truevalue="--header-line" falsevalue="" checked="False"
+               label="Display a header line indicating sample IDs"/>
 
         <expand macro="input_database" />
     </inputs>
@@ -51,7 +52,8 @@
             <param name="show_zeros" value="--show-zeros"/>
             <param name="header_line" value="--header-line"/>
             <param name="kraken_database" value="test_db"/>
-            <output name="output_report" file="kraken_mpa_report_test1_output.tab" ftype="tabular"/>
+
+            <output name="output_report" ftype="tabular" file="kraken_mpa_report_test1_output.tab" />
         </test>
     </tests>
     <help>
diff --git a/tool_collections/kraken/kraken_report/kraken-report.xml b/tool_collections/kraken/kraken_report/kraken-report.xml
index fb783f766cf..8d0919b2334 100644
--- a/tool_collections/kraken/kraken_report/kraken-report.xml
+++ b/tool_collections/kraken/kraken_report/kraken-report.xml
@@ -1,22 +1,22 @@
 <tool id="kraken-report" name="Kraken-report" version="1.2.1">
-    <description>
-        view a sample report of your classification
-    </description>
+    <description>view sample report of a classification</description>
     
     <macros>
         <import>macros.xml</import>
     </macros>
     <expand macro="requirements" />
-    <expand macro="stdio" />
     <expand macro="version_command" />
-    <command><![CDATA[
+    <command detect_errors="exit_code"><![CDATA[
         @SET_DATABASE_PATH@ &&
         
-        kraken-report @INPUT_DATABASE@ '${kraken_output}' > '$output_report'
+        kraken-report
+            @INPUT_DATABASE@
+            '${kraken_output}' >
+            '$output_report'
     ]]></command>
     <inputs>
-        <param name="kraken_output" format="tabular" label="Kraken output" type="data" help="Select taxonomy classification produced by kraken"/>
-        
+        <param name="kraken_output" type="data" format="tabular" label="Kraken output" help="Select taxonomy classification produced by kraken"/>
+
         <expand macro="input_database" />
     </inputs>
     <outputs>
@@ -26,7 +26,7 @@
         <test>
             <param name="kraken_output" value="kraken_report_test1.tab" ftype="tabular"/>
             <param name="kraken_database" value="test_db"/>
-            <output name="output_report" file="kraken_report_test1_output.tab" ftype="tabular"/>
+            <output name="output_report" ftype="tabular" file="kraken_report_test1_output.tab"/>
         </test>
     </tests>
 
diff --git a/tool_collections/kraken/kraken_translate/kraken-translate.xml b/tool_collections/kraken/kraken_translate/kraken-translate.xml
index 9b0876d4751..d191b3e9e10 100644
--- a/tool_collections/kraken/kraken_translate/kraken-translate.xml
+++ b/tool_collections/kraken/kraken_translate/kraken-translate.xml
@@ -1,14 +1,11 @@
 <tool id="kraken-translate" name="Kraken-translate" version="1.2.1">
-    <description>
-        convert taxonomy IDs to names
-    </description>
+    <description>convert taxonomy IDs to names</description>
     <macros>
         <import>macros.xml</import>
     </macros>
     <expand macro="requirements" />
-    <expand macro="stdio" />
     <expand macro="version_command" />
-    <command><![CDATA[
+    <command detect_errors="exit_code"><![CDATA[
         @SET_DATABASE_PATH@ &&
         
         kraken-translate
@@ -18,12 +15,15 @@
             '${translated}'
     ]]></command>
     <inputs>
-        <param format="tabular" label="Kraken output" name="input" type="data" help="Select taxonomy classification produced by kraken"/>
-        <param name="mpa_format" truevalue="--mpa-format" falsevalue="" type="boolean" label="Restrict labels to standard rank assignments" />
+        <param name="input" type="data" format="tabular" label="Kraken output"
+               help="Select taxonomy classification produced by kraken"/>
+        <param name="mpa_format" type="boolean" truevalue="--mpa-format" falsevalue=""
+               label="Restrict labels to standard rank assignments" />
+
         <expand macro="input_database" />
     </inputs>
     <outputs>
-        <data format="tabular" label="${tool.name} on ${on_string}: Translated classification" name="translated" />
+        <data name="translated" format="tabular" label="${tool.name} on ${on_string}: Translated classification"/>
     </outputs>
     <tests>
         <test>
diff --git a/tool_collections/kraken/macros.xml b/tool_collections/kraken/macros.xml
index 37d8c429a72..1a47b71a032 100644
--- a/tool_collections/kraken/macros.xml
+++ b/tool_collections/kraken/macros.xml
@@ -5,13 +5,10 @@
             <requirement type="package" version="0.10.6_eaf8fb68">kraken</requirement>
         </requirements>
     </xml>
-    <xml name="stdio">
-        <stdio>
-            <exit_code description="Tool exception" level="fatal" range="1:" />
-        </stdio>
-    </xml>
     <xml name="version_command">
-        <version_command>export LC_ALL=C &amp;&amp; kraken --version</version_command>
+        <version_command><![CDATA[
+            export LC_ALL=C && kraken --version
+        ]]></version_command>
     </xml>
     <xml name="input_database">
         <param label="Select a Kraken database" name="kraken_database" type="select">

From 8215ba70f7ca2d894da59788f31d50fd17c1ed82 Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Fri, 24 Feb 2017 21:50:22 +0100
Subject: [PATCH 23/40] samtools: small change

---
 .../samtools_stats/samtools_stats.xml         | 62 ++++++++++---------
 1 file changed, 32 insertions(+), 30 deletions(-)

diff --git a/tool_collections/samtools/samtools_stats/samtools_stats.xml b/tool_collections/samtools/samtools_stats/samtools_stats.xml
index 4b75ac06966..15b7b650f8b 100644
--- a/tool_collections/samtools/samtools_stats/samtools_stats.xml
+++ b/tool_collections/samtools/samtools_stats/samtools_stats.xml
@@ -9,45 +9,47 @@
     <command><![CDATA[
         #if $use_reference.use_ref_selector == "yes":
             #if $use_reference.reference_source.reference_source_selector == "history":
-               ln -s "${use_reference.reference_source.ref_file}" && samtools faidx `basename "${use_reference.reference_source.ref_file}"` &&
+               ln -s '${use_reference.reference_source.ref_file}' &&
+               samtools faidx `basename "${use_reference.reference_source.ref_file}"` &&
             #end if
         #end if
         
         samtools stats
-        "${input_file}"
-        --coverage ${coverage_min},${coverage_max},${coverage_step}
-        ${remove_dups}
-        #if str( $filter_by_flags.filter_flags ) == "filter":
-            #if $filter_by_flags.require_flags:
-                --required-flag ${sum([int(flag) for flag in str($filter_by_flags.require_flags).split(',')])}
+            '${input_file}'
+            --coverage ${coverage_min},${coverage_max},${coverage_step}
+            ${remove_dups}
+            #if str( $filter_by_flags.filter_flags ) == "filter":
+                #if $filter_by_flags.require_flags:
+                    --required-flag ${sum([int(flag) for flag in str($filter_by_flags.require_flags).split(',')])}
+                #end if
+                #if $filter_by_flags.exclude_flags:
+                    --filtering-flag ${sum([int(flag) for flag in str($filter_by_flags.exclude_flags).split(',')])}
+                #end if
             #end if
-            #if $filter_by_flags.exclude_flags:
-                --filtering-flag ${sum([int(flag) for flag in str($filter_by_flags.exclude_flags).split(',')])}
-            #end if
-        #end if
-        --GC-depth ${gc_depth}
-        --insert-size ${insert_size}
+            --GC-depth ${gc_depth}
+            --insert-size ${insert_size}
 
-        ## The code below is commented out because using -I/--id options causes the following exception
-        ## Samtools-htslib: init_group_id() header parsing not yet implemented
+            ## The code below is commented out because using -I/--id options causes the following exception
+            ## Samtools-htslib: init_group_id() header parsing not yet implemented
 
-        ##if str($read_group) != "":
-        ##    -I "${read_group}"
-        ##end if
+            ##if str($read_group) != "":
+            ##    -I "${read_group}"
+            ##end if
 
-        #if str($read_length) != "0":
-            --read-length "${read_length}"
-        #end if
-        --most-inserts ${most_inserts}
-        --trim-quality ${trim_quality}
-        #if $use_reference.use_ref_selector == "yes":
-            #if $use_reference.reference_source.reference_source_selector != "history":
-                --ref-seq "${use_reference.reference_source.ref_file.fields.path}"
-            #else:
-                --ref-seq "${use_reference.reference_source.ref_file}"
+            #if str($read_length) != "0":
+                --read-length "${read_length}"
             #end if
-        #end if
-        > "${output}"
+            --most-inserts ${most_inserts}
+            --trim-quality ${trim_quality}
+            #if $use_reference.use_ref_selector == "yes":
+                #if $use_reference.reference_source.reference_source_selector != "history":
+                    --ref-seq "${use_reference.reference_source.ref_file.fields.path}"
+                #else:
+                    --ref-seq "${use_reference.reference_source.ref_file}"
+                #end if
+            #end if
+            > "${output}"
+        
         #if $split_output.split_output_selector == "yes":
             #set outputs_to_split = str($split_output.generate_tables).split(',')
             && mkdir split && echo ${split_output.generate_tables} &&

From 4dc44cee704327da2de77e28cb578a85676f557b Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Sat, 25 Feb 2017 08:08:10 +0100
Subject: [PATCH 24/40] samtools: a few more textual changes

---
 .../bam_to_cram/samtools_bam_to_cram.xml      |  6 +--
 .../samtools/bam_to_sam/bam_to_sam.xml        | 28 ++++++------
 .../samtools_bedcov/samtools_bedcov.xml       | 19 ++++----
 .../samtools_calmd/samtools_calmd.xml         | 16 ++++---
 .../samtools_idxstats/samtools_idxstats.xml   |  4 +-
 .../samtools_mpileup/samtools_mpileup.xml     | 12 +++--
 .../samtools_phase/samtools_phase.xml         |  5 ++-
 .../samtools_rmdup/samtools_rmdup.xml         |  7 +--
 .../samtools/samtools_sort/samtools_sort.xml  |  4 +-
 .../samtools_split/samtools_split.xml         |  8 ++--
 .../samtools_stats/samtools_stats.xml         | 45 ++++++++++---------
 11 files changed, 78 insertions(+), 76 deletions(-)

diff --git a/tool_collections/samtools/bam_to_cram/samtools_bam_to_cram.xml b/tool_collections/samtools/bam_to_cram/samtools_bam_to_cram.xml
index bd58c0cccee..0ac0ef7ae79 100644
--- a/tool_collections/samtools/bam_to_cram/samtools_bam_to_cram.xml
+++ b/tool_collections/samtools/bam_to_cram/samtools_bam_to_cram.xml
@@ -8,8 +8,7 @@
     <expand macro="stdio"/>
     <expand macro="version_command"/>
 
-    <command>
-    <![CDATA[
+    <command><![CDATA[
         #if str( $input_alignment.metadata.bam_index ) != "None":
             ln
                 -f
@@ -34,8 +33,7 @@
             #if $parameter_regions.target_region == "region"
                 '${parameter_regions.region_string}'
             #end if
-    ]]>
-    </command>
+    ]]></command>
 
     <inputs>
         <param name="input_alignment" type="data" format="bam,sam" label="BAM (or SAM) alignment file"/>
diff --git a/tool_collections/samtools/bam_to_sam/bam_to_sam.xml b/tool_collections/samtools/bam_to_sam/bam_to_sam.xml
index 66ee228a974..51ed13092db 100644
--- a/tool_collections/samtools/bam_to_sam/bam_to_sam.xml
+++ b/tool_collections/samtools/bam_to_sam/bam_to_sam.xml
@@ -1,16 +1,16 @@
 <tool id="bam_to_sam" name="BAM-to-SAM" version="2.0">
-  <description>convert BAM to SAM</description>  
+    <description>convert BAM to SAM</description>  
 
-  <macros>
-    <import>macros.xml</import>
-  </macros>
-  <expand macro="requirements"/>
-  <expand macro="stdio"/>
-  <expand macro="version_command"/>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"/>
+    <expand macro="stdio"/>
+    <expand macro="version_command"/>
 
-  <command><![CDATA[
-    samtools view -o '${output1}' ${header} '${input1}'
-  ]]></command>
+    <command><![CDATA[
+        samtools view -o '${output1}' ${header} '${input1}'
+    ]]></command>
 
     <inputs>
         <param format="bam" name="input1" type="data" label="BAM File to Convert" />
@@ -41,8 +41,7 @@
             <output file="bam_to_sam_out3.sam" name="output1" />
         </test>
     </tests>
-    <help>
-<![CDATA[
+    <help><![CDATA[
 
 **What it does**
 
@@ -50,7 +49,6 @@ Converts BAM dataset to SAM using ``samtools view`` command::
 
  samtools view -o [OUTPUT SAM] [-h|-H] [INPUT BAM] 
 
-]]>
-  </help>
-  <expand macro="citations"/>
+    ]]></help>
+    <expand macro="citations"/>
 </tool>
diff --git a/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml b/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml
index 86a5fca3314..2172e5d2e00 100644
--- a/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml
+++ b/tool_collections/samtools/samtools_bedcov/samtools_bedcov.xml
@@ -1,9 +1,9 @@
 <tool id="samtools_bedcov" name="BedCov" version="2.0">
-  <description>calculate read depth for a set of genomic intervals</description>
-  <macros>
-    <import>macros.xml</import>
-  </macros>
-  <expand macro="requirements"/>
+    <description>calculate read depth for a set of genomic intervals</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements"/>
     <expand macro="stdio"/>
     <expand macro="version_command"/>
     <command><![CDATA[
@@ -19,11 +19,10 @@
                 '${i}.bam'
             #end for
             > '${output}'
-        ]]>
-    </command>
+    ]]></command>
     <inputs>
-        <param name="input_bed" type="data" format="bed" label="BED file" />
-        <param name="input_bams" type="data" format="bam" label="BAM file" multiple="true" />
+        <param name="input_bed" type="data" format="bed" label="Genemic intervals (in BED format)" />
+        <param name="input_bams" type="data" format="bam" multiple="true" label="BAM file(s)" />
     </inputs>
     <outputs>
         <data format="tabular" name="output" label="${tool.name} on ${on_string}" />
@@ -48,6 +47,6 @@ Calculates read depth for regions listed in a BED dataset using ``samtools bedco
  samtools bedcov [INPUT BED] [INPUT BAM1] ... [INPUT BAMn] > [OUTPUT]
 
     </help>
-    <expand macro="citations"></expand>
+    <expand macro="citations"/>
 </tool>
 
diff --git a/tool_collections/samtools/samtools_calmd/samtools_calmd.xml b/tool_collections/samtools/samtools_calmd/samtools_calmd.xml
index 62ecc4b2ff7..c7eb1450694 100644
--- a/tool_collections/samtools/samtools_calmd/samtools_calmd.xml
+++ b/tool_collections/samtools/samtools_calmd/samtools_calmd.xml
@@ -9,16 +9,20 @@
     <command><![CDATA[
         #if str( $reference_source.reference_source_selector ) == "history":
             #set ref_fa = 'ref.fa'
-            ln -s "${reference_source.ref_fasta}" ref.fa && samtools faidx ref.fa &&
+            ln -s "${reference_source.ref_fasta}" ref.fa &&
+            samtools faidx ref.fa &&
         #else:
             #set ref_fa = str( $reference_source.ref_fasta.fields.path )
         #end if
+        
         samtools calmd
-        #if str($option_set.option_sets) == 'advanced':
-            $option_set.change_identical $option_set.modify_quality $option_set.compute_cap $option_set.extended_baq
-        #end if
-        -b "$input_bam" "$ref_fa" > "$calmd_output" ]]>
-    </command>
+            #if str($option_set.option_sets) == 'advanced':
+                $option_set.change_identical $option_set.modify_quality $option_set.compute_cap $option_set.extended_baq
+            #end if
+            -b "$input_bam"
+            "$ref_fa"
+            > "$calmd_output"
+    ]]></command>
     <inputs>
         <param format="bam" name="input_bam" type="data" label="BAM file to recalculate" />
         <conditional name="reference_source">
diff --git a/tool_collections/samtools/samtools_idxstats/samtools_idxstats.xml b/tool_collections/samtools/samtools_idxstats/samtools_idxstats.xml
index 2473539d797..820b96e299d 100644
--- a/tool_collections/samtools/samtools_idxstats/samtools_idxstats.xml
+++ b/tool_collections/samtools/samtools_idxstats/samtools_idxstats.xml
@@ -1,5 +1,5 @@
 <tool id="samtools_idxstats" name="IdxStats" version="2.0">
-    <description>tabulate mapping statistics for BAM dataset</description>
+    <description>reports stats of the BAM index file</description>
     <macros>
       <import>macros.xml</import>
     </macros>
@@ -7,7 +7,7 @@
     <expand macro="stdio"/>
     <expand macro="version_command"/>
     <command><![CDATA[
-        ln -f -s '$input.metadata.bam_index' '\$(basename $input).bai' &&
+        ln -f -s '$input.metadata.bam_index' "\$(basename $input).bai" &&
         
         samtools idxstats '${input}' > '${output}'
     ]]></command>
diff --git a/tool_collections/samtools/samtools_mpileup/samtools_mpileup.xml b/tool_collections/samtools/samtools_mpileup/samtools_mpileup.xml
index 8490749cb8b..6c5c586414b 100644
--- a/tool_collections/samtools/samtools_mpileup/samtools_mpileup.xml
+++ b/tool_collections/samtools/samtools_mpileup/samtools_mpileup.xml
@@ -6,15 +6,14 @@
     <expand macro="requirements" />
     <expand macro="stdio" />
     <expand macro="version_command" />
-    <command>
-    <![CDATA[
+    <command><![CDATA[
     #for $bam_count, $input_bam in enumerate( $reference_source.input_bam ):
-        ln -s "${input_bam}" "localbam_${bam_count}.bam" &&
-        ln -s "${input_bam.metadata.bam_index}" "localbam_${bam_count}.bam.bai" &&
+        ln -s '${input_bam}' 'localbam_${bam_count}.bam' &&
+        ln -s '${input_bam.metadata.bam_index}' 'localbam_${bam_count}.bam.bai' &&
     #end for
 
     #if $reference_source.reference_source_selector == "history":
-       ln -s "${reference_source.ref_file}" &&
+       ln -s '${reference_source.ref_file}' &&
        samtools faidx `basename "${reference_source.ref_file}"` &&
     #end if
 
@@ -91,8 +90,7 @@
         ${genotype_likelihood_computation_type.output_mapping_quality}
     #end if
     --output "$output_mpileup" 2> "$output_log"
-    ]]>
-    </command>
+    ]]></command>
 
     <configfiles>
         <configfile name="excluded_read_groups">
diff --git a/tool_collections/samtools/samtools_phase/samtools_phase.xml b/tool_collections/samtools/samtools_phase/samtools_phase.xml
index d63644659fe..e1037845581 100644
--- a/tool_collections/samtools/samtools_phase/samtools_phase.xml
+++ b/tool_collections/samtools/samtools_phase/samtools_phase.xml
@@ -16,7 +16,10 @@
             -D $option_set.read_depth
             ${option_set.drop_ambiguous}
         #else
-            -k 13 -q 37 -Q 13 -D 256
+            -k 13
+            -q 37
+            -Q 13
+            -D 256
         #end if
         '$input_bam' > '$phase_sets'
     ]]></command>
diff --git a/tool_collections/samtools/samtools_rmdup/samtools_rmdup.xml b/tool_collections/samtools/samtools_rmdup/samtools_rmdup.xml
index a8ebc2755bb..d7834cabba6 100644
--- a/tool_collections/samtools/samtools_rmdup/samtools_rmdup.xml
+++ b/tool_collections/samtools/samtools_rmdup/samtools_rmdup.xml
@@ -1,11 +1,8 @@
-<tool id="samtools_rmdup" name="RmDup" version="2.0">
+<tool id="samtools_rmdup" name="RmDup" version="2.1">
     <description>remove PCR duplicates</description>
     <macros>
         <import>macros.xml</import>
     </macros>
-    <requirements>
-        <requirement type="package" version="0.1.19">samtools</requirement>
-    </requirements>
     
     <expand macro="stdio"/>
     <expand macro="version_command"/>
@@ -30,7 +27,7 @@
                 <option value="SE">BAM is single-end (-s)</option>
             </param>
             <when value="PE">
-                <param name="force_se" type="boolean" label="Treat as single-end" help="-S" truevalue="-S" falsevalue="" checked="False"/>
+                <param name="force_se" argument="-S" type="boolean" truevalue="-S" falsevalue="" checked="False" label="Treat as single-end"/>
             </when>
             <when value="SE" /> <!-- No extra parameters here -->
         </conditional>
diff --git a/tool_collections/samtools/samtools_sort/samtools_sort.xml b/tool_collections/samtools/samtools_sort/samtools_sort.xml
index 80c17ef2203..48f744af243 100644
--- a/tool_collections/samtools/samtools_sort/samtools_sort.xml
+++ b/tool_collections/samtools/samtools_sort/samtools_sort.xml
@@ -1,7 +1,7 @@
 <tool id="samtools_sort" name="Sort" version="2.0">
-    <description>BAM dataset</description>
+    <description>order of storing aligned sequences</description>
     <macros>
-      <import>macros.xml</import>
+        <import>macros.xml</import>
     </macros>
     <expand macro="requirements"/>
     <expand macro="stdio"/>
diff --git a/tool_collections/samtools/samtools_split/samtools_split.xml b/tool_collections/samtools/samtools_split/samtools_split.xml
index 293fab0efa6..6d23f4f781f 100644
--- a/tool_collections/samtools/samtools_split/samtools_split.xml
+++ b/tool_collections/samtools/samtools_split/samtools_split.xml
@@ -14,7 +14,7 @@
             -u "${output}"
         #end if
         "${input_bam}"
-        ]]></command>
+    ]]></command>
     <inputs>
         <param name="input_bam" type="data" format="bam" label="BAM file" />
         <param name="header" type="data" format="bam,sam" label="Replace header in output file" optional="True" />
@@ -34,14 +34,14 @@
             </output>
         </test>
     </tests>
-    <help>
+    <help><![CDATA[
 **What it does**
 
 Splits BAM files on readgroups.
 
 This tool is based on ``samtools split`` command. It will generate multiple output datasets for each redagroup from the input dataset. 
 
-    </help>
-    <expand macro="citations"></expand>
+    ]]></help>
+    <expand macro="citations"/>
 </tool>
 
diff --git a/tool_collections/samtools/samtools_stats/samtools_stats.xml b/tool_collections/samtools/samtools_stats/samtools_stats.xml
index 15b7b650f8b..d6d2ab18f0d 100644
--- a/tool_collections/samtools/samtools_stats/samtools_stats.xml
+++ b/tool_collections/samtools/samtools_stats/samtools_stats.xml
@@ -13,11 +13,12 @@
                samtools faidx `basename "${use_reference.reference_source.ref_file}"` &&
             #end if
         #end if
-        
+
         samtools stats
             '${input_file}'
             --coverage ${coverage_min},${coverage_max},${coverage_step}
             ${remove_dups}
+
             #if str( $filter_by_flags.filter_flags ) == "filter":
                 #if $filter_by_flags.require_flags:
                     --required-flag ${sum([int(flag) for flag in str($filter_by_flags.require_flags).split(',')])}
@@ -26,12 +27,12 @@
                     --filtering-flag ${sum([int(flag) for flag in str($filter_by_flags.exclude_flags).split(',')])}
                 #end if
             #end if
+
             --GC-depth ${gc_depth}
             --insert-size ${insert_size}
 
             ## The code below is commented out because using -I/--id options causes the following exception
             ## Samtools-htslib: init_group_id() header parsing not yet implemented
-
             ##if str($read_group) != "":
             ##    -I "${read_group}"
             ##end if
@@ -39,20 +40,23 @@
             #if str($read_length) != "0":
                 --read-length "${read_length}"
             #end if
+
             --most-inserts ${most_inserts}
             --trim-quality ${trim_quality}
+
             #if $use_reference.use_ref_selector == "yes":
                 #if $use_reference.reference_source.reference_source_selector != "history":
-                    --ref-seq "${use_reference.reference_source.ref_file.fields.path}"
+                    --ref-seq '${use_reference.reference_source.ref_file.fields.path}'
                 #else:
-                    --ref-seq "${use_reference.reference_source.ref_file}"
+                    --ref-seq '${use_reference.reference_source.ref_file}'
                 #end if
             #end if
             > "${output}"
         
         #if $split_output.split_output_selector == "yes":
             #set outputs_to_split = str($split_output.generate_tables).split(',')
-            && mkdir split && echo ${split_output.generate_tables} &&
+            && mkdir split &&
+            echo ${split_output.generate_tables} &&
 
             #if 'sn' in $outputs_to_split:
                 echo "# Summary Numbers" > "split/Summary numbers.tab" &&
@@ -128,18 +132,19 @@
         ]]></command>
     <inputs>
         <param name="input_file" type="data" format="sam,bam" label="BAM file" />
-        <param name="coverage_min" type="integer" value="1" label="Minimum coverage" help="minimum coverage value for --coverage option"/>
-        <param name="coverage_max" type="integer" value="1000" label="Maximum coverage" help="maximum coverage value for --coverage option"/>
-        <param name="coverage_step" type="integer" value="1" label="Coverage step" help="step value for --coverage option"/>
-        <param name="remove_dups" type="boolean" truevalue="--remove-dups" falsevalue="" checked="False" label="Exclude reads marked as duplicates" help="--remove-dups; default = False"/>
+        <param name="coverage_min" type="integer" value="1" label="Minimum coverage" help="minimum coverage"/>
+        <param name="coverage_max" type="integer" value="1000" label="Maximum coverage" help="maximum coverage"/>
+        <param name="coverage_step" type="integer" value="1" label="Coverage step" help="step value for coverage"/>
+        <param name="remove_dups" argument="--remove-dups" type="boolean" truevalue="--remove-dups" falsevalue="" checked="False"
+               label="Exclude reads marked as duplicates" help="default = False"/>
         <conditional name="split_output">
-            <param name="split_output_selector" type="select" label="Output" help="Select between a single output or separate outputs for each statistics">
-                <option value="no" selected="True">a single summary file</option>
-                <option value="yes">separate datasets for each statistics</option>
+            <param name="split_output_selector" type="select" label="Output" help="Select between one single output or separate outputs for each statistics">
+                <option value="no" selected="True"One single summary file</option>
+                <option value="yes">Separate datasets for each statistic</option>
             </param>
             <when value="no" />
             <when value="yes">
-                <param name="generate_tables" type="select" display="checkboxes" multiple="True" label="Statistics to extract">
+                <param name="generate_tables" type="select" display="checkboxes" multiple="True" label="Desired output files">
                     <option value="sn">Summary numbers</option>
                     <option value="ffq">First Fragment Qualities</option>
                     <option value="lfq">Last Fragment Qualities</option>
@@ -157,12 +162,12 @@
             </when>
         </conditional>
         <conditional name="filter_by_flags">
-            <param name="filter_flags" type="select" label="Set filter by flags" help="-f and -F options">
+            <param name="filter_flags" type="select" label="Filter by SAM flags" help="More info on the flags: https://samtools.github.io/hts-specs/SAMv1.pdf">
                 <option value="nofilter" selected="True">Do not filter</option>
                 <option value="filter">Filter by flags to exclude or require</option>
             </param>
             <when value="filter">
-                <param name="require_flags" type="select" display="checkboxes" multiple="True" label="Require" help="-f">
+                <param name="require_flags" argument="-f" type="select" display="checkboxes" multiple="True" label="Require">
                     <option value="1">Read is paired</option>
                     <option value="2">Read is mapped in a proper pair</option>
                     <option value="4">The read is unmapped</option>
@@ -175,7 +180,7 @@
                     <option value="512">The read fails platform/vendor quality checks</option>
                     <option value="1024">The read is a PCR or optical duplicate</option>
                 </param>
-                <param name="exclude_flags" type="select" display="checkboxes" multiple="True" label="Exclude" help="-F">
+                <param name="exclude_flags" argument="-F" type="select" display="checkboxes" multiple="True" label="Exclude">
                     <option value="1">Read is paired</option>
                     <option value="2">Read is mapped in a proper pair</option>
                     <option value="4">The read is unmapped</option>
@@ -192,7 +197,7 @@
             <when value="nofilter" />
 
         </conditional>
-        <param name="gc_depth" type="float" value="20000" label="GC-depth bin size" help="--GC-depth; decreasing bin size increases memory requirement; default = 20000.0"/>
+        <param name="gc_depth" argument="--GC-depth" type="float" value="20000" label="Bin size GC-depth" help="Decreasing bin size increases memory requirement; default = 20000.0"/>
         <param name="insert_size" type="integer" value="8000" label="Maximum insert size" help="--insert-size; default = 8000"/>
 
         <!--
@@ -212,9 +217,9 @@
 
         -->
 
-        <param name="read_length" type="integer" value="0" label="Minimum read length to generate statistics for" help="--read-length; default = no cutoff"/>
-        <param name="most_inserts" type="float" value="0.99" label="Report only the main part of inserts" help="--most-inserts; default = 0.99"/>
-        <param name="trim_quality" type="integer" value="0" label="BWA trim parameter" help="--trim-quality; default = 0"/>
+        <param name="read_length" argument="--read-length" type="integer" value="0" label="Minimum read length to generate statistics for" help="default = no cutoff"/>
+        <param name="most_inserts" argument="--most-inserts" type="float" value="0.99" label="Report only the main part of inserts" help="default = 0.99"/>
+        <param name="trim_quality" argument="--trim-quality" type="integer" value="0" label="BWA trim parameter" help="default = 0"/>
 
         <conditional name="use_reference">
             <param name="use_ref_selector" type="select" label="Use reference sequence" help="--ref-seq; required for GC-depth and mismatches-per-cycle calculation">

From 361b7a9faf72bac0372cd3a3db15070788ba25a6 Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Sat, 25 Feb 2017 08:11:08 +0100
Subject: [PATCH 25/40] samtoools: typo

---
 tool_collections/samtools/samtools_stats/samtools_stats.xml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/tool_collections/samtools/samtools_stats/samtools_stats.xml b/tool_collections/samtools/samtools_stats/samtools_stats.xml
index d6d2ab18f0d..34c48e6fd7b 100644
--- a/tool_collections/samtools/samtools_stats/samtools_stats.xml
+++ b/tool_collections/samtools/samtools_stats/samtools_stats.xml
@@ -139,7 +139,7 @@
                label="Exclude reads marked as duplicates" help="default = False"/>
         <conditional name="split_output">
             <param name="split_output_selector" type="select" label="Output" help="Select between one single output or separate outputs for each statistics">
-                <option value="no" selected="True"One single summary file</option>
+                <option value="no" selected="True">One single summary file</option>
                 <option value="yes">Separate datasets for each statistic</option>
             </param>
             <when value="no" />

From abc67ae3353517d3e11018e55a66b78a91b29fb3 Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Mon, 27 Feb 2017 08:37:11 +0100
Subject: [PATCH 26/40] samtools idxstats: fix symlinking bai file

---
 .../samtools/samtools_idxstats/samtools_idxstats.xml            | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/tool_collections/samtools/samtools_idxstats/samtools_idxstats.xml b/tool_collections/samtools/samtools_idxstats/samtools_idxstats.xml
index 820b96e299d..5297ad61b0f 100644
--- a/tool_collections/samtools/samtools_idxstats/samtools_idxstats.xml
+++ b/tool_collections/samtools/samtools_idxstats/samtools_idxstats.xml
@@ -7,7 +7,7 @@
     <expand macro="stdio"/>
     <expand macro="version_command"/>
     <command><![CDATA[
-        ln -f -s '$input.metadata.bam_index' "\$(basename $input).bai" &&
+        ln -f -s '${input.metadata.bam_index}' '${input}.bai' &&
         
         samtools idxstats '${input}' > '${output}'
     ]]></command>

From cde95566ed51b74d9c186c274956f63f732b246e Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Mon, 27 Feb 2017 15:50:20 +0100
Subject: [PATCH 27/40] More changes thanks to Nicola

---
 tool_collections/kraken/kraken/kraken.xml                   | 3 +--
 tool_collections/samtools/samtools_rmdup/samtools_rmdup.xml | 5 +++--
 tool_collections/samtools/samtools_stats/samtools_stats.xml | 2 +-
 3 files changed, 5 insertions(+), 5 deletions(-)

diff --git a/tool_collections/kraken/kraken/kraken.xml b/tool_collections/kraken/kraken/kraken.xml
index 660ef89c1cf..10e5f3039e7 100644
--- a/tool_collections/kraken/kraken/kraken.xml
+++ b/tool_collections/kraken/kraken/kraken.xml
@@ -93,8 +93,7 @@
             <when value="no"/><!-- Do absolutely nothing -->
         </conditional>
 
-        <param name="only_classified_output" argument="--only-classified-output" type="boolean" checked="False"
-               truevalue="--only-classified-output" falsevalue=""
+        <param name="only_classified_output" argument="--only-classified-output" type="boolean" checked="False" truevalue="--only-classified-output" falsevalue=""
                label="Print no Kraken output for unclassified sequences"/>
 
         <expand macro="input_database" />
diff --git a/tool_collections/samtools/samtools_rmdup/samtools_rmdup.xml b/tool_collections/samtools/samtools_rmdup/samtools_rmdup.xml
index d7834cabba6..482e7123f2f 100644
--- a/tool_collections/samtools/samtools_rmdup/samtools_rmdup.xml
+++ b/tool_collections/samtools/samtools_rmdup/samtools_rmdup.xml
@@ -3,10 +3,11 @@
     <macros>
         <import>macros.xml</import>
     </macros>
-    
+
     <expand macro="stdio"/>
     <expand macro="version_command"/>
-    
+    <expand macro="requirements" />
+
     <command><![CDATA[
     samtools rmdup 
         #if str( $bam_paired_end_type.bam_paired_end_type_selector ) == "PE"
diff --git a/tool_collections/samtools/samtools_stats/samtools_stats.xml b/tool_collections/samtools/samtools_stats/samtools_stats.xml
index 34c48e6fd7b..018bf49762b 100644
--- a/tool_collections/samtools/samtools_stats/samtools_stats.xml
+++ b/tool_collections/samtools/samtools_stats/samtools_stats.xml
@@ -197,7 +197,7 @@
             <when value="nofilter" />
 
         </conditional>
-        <param name="gc_depth" argument="--GC-depth" type="float" value="20000" label="Bin size GC-depth" help="Decreasing bin size increases memory requirement; default = 20000.0"/>
+        <param name="gc_depth" argument="--GC-depth" type="float" value="20000" label="Size of GC-depth bins" help="Decreasing bin size increases memory requirement; default = 20000.0"/>
         <param name="insert_size" type="integer" value="8000" label="Maximum insert size" help="--insert-size; default = 8000"/>
 
         <!--

From a44101b17969ee3ad1dd057f2416ba92f8c81fd1 Mon Sep 17 00:00:00 2001
From: Martin Cech <cech.marten@gmail.com>
Date: Tue, 28 Feb 2017 13:28:17 -0500
Subject: [PATCH 28/40] fix shedyaml to point at iuc repo

---
 tool_collections/kraken/kraken_filter/.shed.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/tool_collections/kraken/kraken_filter/.shed.yml b/tool_collections/kraken/kraken_filter/.shed.yml
index 89bc8f6636d..37aedb65a83 100644
--- a/tool_collections/kraken/kraken_filter/.shed.yml
+++ b/tool_collections/kraken/kraken_filter/.shed.yml
@@ -12,5 +12,5 @@ long_description: |
   and a novel classification algorithm.
 name: kraken_filter
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/blob/master/tool_collections/kraken/kraken_filter/
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/blob/master/tool_collections/kraken/kraken_filter/
 type: unrestricted

From 6e1b06018da158ae75dfaddcd9a97837b2012b47 Mon Sep 17 00:00:00 2001
From: Martin Cech <cech.marten@gmail.com>
Date: Tue, 28 Feb 2017 13:28:43 -0500
Subject: [PATCH 29/40] fix shedyaml to point at iuc repo

---
 tool_collections/kraken/kraken_report/.shed.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/tool_collections/kraken/kraken_report/.shed.yml b/tool_collections/kraken/kraken_report/.shed.yml
index 2dea471fdf4..344d485e952 100644
--- a/tool_collections/kraken/kraken_report/.shed.yml
+++ b/tool_collections/kraken/kraken_report/.shed.yml
@@ -12,5 +12,5 @@ long_description: |
   and a novel classification algorithm.
 name: kraken_report
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/blob/master/tool_collections/kraken/kraken_report/
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/blob/master/tool_collections/kraken/kraken_report/
 type: unrestricted

From 6c16017b0a17b4911e4d3586ce72331359be935d Mon Sep 17 00:00:00 2001
From: Martin Cech <cech.marten@gmail.com>
Date: Tue, 28 Feb 2017 13:29:05 -0500
Subject: [PATCH 30/40] fix shedyaml to point at iuc repo

---
 tool_collections/kraken/kraken_translate/.shed.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/tool_collections/kraken/kraken_translate/.shed.yml b/tool_collections/kraken/kraken_translate/.shed.yml
index 37130f1d68e..6e54a30ca82 100644
--- a/tool_collections/kraken/kraken_translate/.shed.yml
+++ b/tool_collections/kraken/kraken_translate/.shed.yml
@@ -12,5 +12,5 @@ long_description: |
   and a novel classification algorithm.
 name: kraken_translate
 owner: devteam
-remote_repository_url: https://github.com/galaxyproject/tools-devteam/blob/master/tool_collections/kraken/kraken_translate/
+remote_repository_url: https://github.com/galaxyproject/tools-iuc/blob/master/tool_collections/kraken/kraken_translate/
 type: unrestricted

From f8fb9deb0aa36e2bda92e2df8a4faac26ebdb722 Mon Sep 17 00:00:00 2001
From: Martin Cech <cech.marten@gmail.com>
Date: Tue, 7 Mar 2017 15:15:31 -0500
Subject: [PATCH 31/40] update rmdup to samtools 1.3 and tiny improvements, all
 thanks to :eagle: :eyes:

---
 .../kraken/kraken_filter/kraken-filter.xml            |  6 +++---
 .../kraken/kraken_report/kraken-report.xml            |  4 ++--
 .../kraken/kraken_translate/kraken-translate.xml      |  4 ++--
 .../samtools/cram_to_bam/samtools_cram_to_bam.xml     |  2 +-
 tool_collections/samtools/macros.xml                  |  2 +-
 .../samtools/samtools_flagstat/samtools_flagstat.xml  | 11 +++++------
 .../samtools/samtools_reheader/samtools_reheader.xml  |  4 ++--
 .../samtools/samtools_rmdup/samtools_rmdup.xml        |  4 ++--
 8 files changed, 18 insertions(+), 19 deletions(-)

diff --git a/tool_collections/kraken/kraken_filter/kraken-filter.xml b/tool_collections/kraken/kraken_filter/kraken-filter.xml
index 8e1c6a13bb4..706cdb528a4 100644
--- a/tool_collections/kraken/kraken_filter/kraken-filter.xml
+++ b/tool_collections/kraken/kraken_filter/kraken-filter.xml
@@ -11,8 +11,8 @@
         kraken-filter
             @INPUT_DATABASE@
             --threshold $threshold
-            '${input}' >
-            '$filtered_output'
+            '${input}'
+            > '$filtered_output'
     ]]></command>
     <inputs>
         <param name="input" type="data" format="tabular" label="Kraken output" help="Select taxonomy classification produced by kraken"/>
@@ -30,7 +30,7 @@
             <param name="threshold" value="0"/>
             <param name="kraken_database" value="test_db"/>
 
-            <output name="output" file="kraken_filter_test1_output.tab" ftype="tabular"/>
+            <output name="filtered_output" file="kraken_filter_test1_output.tab" ftype="tabular"/>
         </test>
     </tests>
 
diff --git a/tool_collections/kraken/kraken_report/kraken-report.xml b/tool_collections/kraken/kraken_report/kraken-report.xml
index 8d0919b2334..062c09897c4 100644
--- a/tool_collections/kraken/kraken_report/kraken-report.xml
+++ b/tool_collections/kraken/kraken_report/kraken-report.xml
@@ -11,8 +11,8 @@
         
         kraken-report
             @INPUT_DATABASE@
-            '${kraken_output}' >
-            '$output_report'
+            '${kraken_output}'
+            > '$output_report'
     ]]></command>
     <inputs>
         <param name="kraken_output" type="data" format="tabular" label="Kraken output" help="Select taxonomy classification produced by kraken"/>
diff --git a/tool_collections/kraken/kraken_translate/kraken-translate.xml b/tool_collections/kraken/kraken_translate/kraken-translate.xml
index d191b3e9e10..44ef1375deb 100644
--- a/tool_collections/kraken/kraken_translate/kraken-translate.xml
+++ b/tool_collections/kraken/kraken_translate/kraken-translate.xml
@@ -11,8 +11,8 @@
         kraken-translate
             @INPUT_DATABASE@
             $mpa_format
-            '${input}' >
-            '${translated}'
+            '${input}'
+            > '${translated}'
     ]]></command>
     <inputs>
         <param name="input" type="data" format="tabular" label="Kraken output"
diff --git a/tool_collections/samtools/cram_to_bam/samtools_cram_to_bam.xml b/tool_collections/samtools/cram_to_bam/samtools_cram_to_bam.xml
index a0080224029..7817b182ab7 100644
--- a/tool_collections/samtools/cram_to_bam/samtools_cram_to_bam.xml
+++ b/tool_collections/samtools/cram_to_bam/samtools_cram_to_bam.xml
@@ -5,8 +5,8 @@
         <import>macros.xml</import>
     </macros>
     <expand macro="requirements"/>
-    <expand macro="version_command"/>
     <expand macro="stdio"/>
+    <expand macro="version_command"/>
 
     <command><![CDATA[
         #if str( $input_alignment.metadata.cram_index ) != "None":
diff --git a/tool_collections/samtools/macros.xml b/tool_collections/samtools/macros.xml
index a4e6d47a246..23c3d1d9e8f 100644
--- a/tool_collections/samtools/macros.xml
+++ b/tool_collections/samtools/macros.xml
@@ -1,7 +1,7 @@
 <macros>
     <xml name="requirements">
         <requirements>
-            <requirement type="package" version="1.2">samtools</requirement>
+            <requirement type="package" version="1.3">samtools</requirement>
             <yield/>
         </requirements>
     </xml>
diff --git a/tool_collections/samtools/samtools_flagstat/samtools_flagstat.xml b/tool_collections/samtools/samtools_flagstat/samtools_flagstat.xml
index 533ce3cdb18..fd90449b80f 100644
--- a/tool_collections/samtools/samtools_flagstat/samtools_flagstat.xml
+++ b/tool_collections/samtools/samtools_flagstat/samtools_flagstat.xml
@@ -17,14 +17,13 @@
     </inputs>
 
     <outputs>
-    <data name="output1" format="txt" />
+      <data name="output1" format="txt" />
     </outputs>
 
     <tests>
         <test>
-          <param name="input1" value="samtools_flagstat_input1.bam" ftype="bam" />
-
-          <output name="output1" file="samtools_flagstat_out1.txt" />
+            <param name="input1" value="samtools_flagstat_input1.bam" ftype="bam" />
+            <output name="output1" file="samtools_flagstat_out1.txt" />
         </test>
     </tests>
     <help>
@@ -47,6 +46,6 @@ Uses ``samtools flagstat`` command to print descriptive information for a BAM da
   0 + 0 with mate mapped to a different chr
   0 + 0 with mate mapped to a different chr (mapQ>=5)
   
-  </help>
-  <expand macro="citations"/>
+    </help>
+    <expand macro="citations"/>
 </tool>
diff --git a/tool_collections/samtools/samtools_reheader/samtools_reheader.xml b/tool_collections/samtools/samtools_reheader/samtools_reheader.xml
index feb2417a234..bc732fb529b 100644
--- a/tool_collections/samtools/samtools_reheader/samtools_reheader.xml
+++ b/tool_collections/samtools/samtools_reheader/samtools_reheader.xml
@@ -9,8 +9,8 @@
     <command><![CDATA[
         samtools reheader
             '${input_header}'
-            '${input_file}' >
-            '${output}'
+            '${input_file}'
+            > '${output}'
     ]]></command>
     <inputs>
         <param name="input_header" type="data" format="sam,bam" label="Select source dataset" help="Header from this dataset will be used to replace header in the target dataset. May be either SAM or BAM dataset."/>
diff --git a/tool_collections/samtools/samtools_rmdup/samtools_rmdup.xml b/tool_collections/samtools/samtools_rmdup/samtools_rmdup.xml
index 482e7123f2f..744fc0f3b41 100644
--- a/tool_collections/samtools/samtools_rmdup/samtools_rmdup.xml
+++ b/tool_collections/samtools/samtools_rmdup/samtools_rmdup.xml
@@ -1,12 +1,12 @@
-<tool id="samtools_rmdup" name="RmDup" version="2.1">
+<tool id="samtools_rmdup" name="RmDup" version="2.2">
     <description>remove PCR duplicates</description>
     <macros>
         <import>macros.xml</import>
     </macros>
 
+    <expand macro="requirements" />
     <expand macro="stdio"/>
     <expand macro="version_command"/>
-    <expand macro="requirements" />
 
     <command><![CDATA[
     samtools rmdup 

From 9bb0b6d403ade06c7ee4ec4655c3df252a6248ca Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Tue, 25 Apr 2017 09:09:05 +0200
Subject: [PATCH 32/40] Make test of cram/bam tools pass

---
 .../samtools/bam_to_cram/test-data/test.cram  | Bin 11571 -> 1549 bytes
 .../samtools_flagstat/samtools_flagstat.xml   |   2 +-
 .../samtools_stats_tool_test_output.html      | 372 ------------------
 3 files changed, 1 insertion(+), 373 deletions(-)
 delete mode 100644 tool_collections/samtools/samtools_stats/samtools_stats_tool_test_output.html

diff --git a/tool_collections/samtools/bam_to_cram/test-data/test.cram b/tool_collections/samtools/bam_to_cram/test-data/test.cram
index b6b94d9599dcba28e1a5a855e1fde104bb38b482..58d3ad62eaca70ddd30e95f72f9cf9873232cc26 100644
GIT binary patch
delta 939
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diff --git a/tool_collections/samtools/samtools_flagstat/samtools_flagstat.xml b/tool_collections/samtools/samtools_flagstat/samtools_flagstat.xml
index fd90449b80f..b78d6feaa43 100644
--- a/tool_collections/samtools/samtools_flagstat/samtools_flagstat.xml
+++ b/tool_collections/samtools/samtools_flagstat/samtools_flagstat.xml
@@ -17,7 +17,7 @@
     </inputs>
 
     <outputs>
-      <data name="output1" format="txt" />
+        <data name="output1" format="txt" />
     </outputs>
 
     <tests>
diff --git a/tool_collections/samtools/samtools_stats/samtools_stats_tool_test_output.html b/tool_collections/samtools/samtools_stats/samtools_stats_tool_test_output.html
deleted file mode 100644
index fbba3d7b786..00000000000
--- a/tool_collections/samtools/samtools_stats/samtools_stats_tool_test_output.html
+++ /dev/null
@@ -1,372 +0,0 @@
-<!DOCTYPE html>
-<html lang="en">
-  <head>
-    <meta charset="utf-8">
-    <meta http-equiv="X-UA-Compatible" content="IE=edge">
-    <meta name="viewport" content="width=device-width, initial-scale=1">
-    <title>Tool Test Results (powered by Planemo)</title>
-
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-        var test_data = {"tests": [{"data": {"status": "failure", "inputs": {"use_reference|reference_source|ref_file": {"src": "hda", "id": "5729865256bc2525"}, "use_reference|reference_source|reference_source_selector": "history", "use_reference|use_ref_selector": "yes", "input_file": {"src": "hda", "id": "2891970512fa2d5a"}}, "output_problems": ["History item  different than expected, difference (using diff):\n( /Users/marten/devel/git/tools-devteam/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out1.tab v. /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpVPmlQNsamtools_stats_out1.tab )\n--- local_file\n+++ history_data\n@@ -1,5 +1,5 @@\n-# This file was produced by samtools stats (1.2+htslib-1.2.1) and can be plotted using plot-bamstats\n-# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /Users/anton/galaxy-git/database/files/000/dataset_9.dat /Users/anton/galaxy-git/database/files/000/dataset_10.dat\n+# This file was produced by samtools stats (1.2+htslib-1.2) and can be plotted using plot-bamstats\n+# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_2.dat /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_1.dat\n # CHK, Checksum\t[2]Read Names\t[3]Sequences\t[4]Qualities\n # CHK, CRC32 of reads which passed filtering followed by addition (32bit overflow)\n CHK\t1bd20fd8\t58ad2167\t29883386\n"], "job": {"inputs": {"ref_file": {"src": "hda", "id": "5729865256bc2525"}, "input_file": {"src": "hda", "id": "2891970512fa2d5a"}}, "update_time": "2015-04-21T21:45:40.695226", "tool_id": "samtools_stats", "outputs": {"output": {"src": "hda", "id": "54f2a3a23292eb07"}}, "stdout": "", "command_line": "ln -s \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_2.dat\" && samtools faidx `basename \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_2.dat\"` && samtools stats \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_1.dat\" --coverage 1,1000,1  --GC-depth 20000.0 --insert-size 8000    --most-inserts 0.99 --trim-quality 0 --ref-seq \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_2.dat\" > \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_3.dat\"", "exit_code": 0, "state": "ok", "create_time": "2015-04-21T21:45:29.298433", "params": {"coverage_max": "\"1000\"", "gc_depth": "\"20000.0\"", "insert_size": "\"8000\"", "most_inserts": "\"0.99\"", "coverage_step": "\"1\"", "dbkey": "\"hg17\"", "coverage_min": "\"1\"", "read_length": "\"0\"", "trim_quality": "\"0\"", "filter_by_flags": "{\"filter_flags\": \"nofilter\", \"__current_case__\": 1}", "split_output": "{\"split_output_selector\": \"no\", \"__current_case__\": 0}", "use_reference": "{\"reference_source\": {\"ref_file\": 2, \"reference_source_selector\": \"history\", \"__current_case__\": 1}, \"use_ref_selector\": \"yes\", \"__current_case__\": 0}", "chromInfo": "\"/Users/marten/devel/git/galaxy/tool-data/shared/ucsc/chrom/hg17.len\"", "remove_dups": "\"False\""}, "stderr": "[fai_load] build FASTA index.\n", "job_metrics": [], "model_class": "Job", "external_id": "64545", "id": "54f2a3a23292eb07", "user_email": "test@bx.psu.edu"}, "problem_log": "Traceback (most recent call last):\n  File \"/System/Library/Frameworks/Python.framework/Versions/2.7/lib/python2.7/unittest/case.py\", line 331, in run\n    testMethod()\n  File \"/Users/marten/devel/git/galaxy/test/functional/test_toolbox.py\", line 268, in test_tool\n    self.do_it( td )\n  File \"/Users/marten/devel/git/galaxy/test/functional/test_toolbox.py\", line 67, in do_it\n    raise e\nJobOutputsError: History item  different than expected, difference (using diff):\n( /Users/marten/devel/git/tools-devteam/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out1.tab v. /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpVPmlQNsamtools_stats_out1.tab )\n--- local_file\n+++ history_data\n@@ -1,5 +1,5 @@\n-# This file was produced by samtools stats (1.2+htslib-1.2.1) and can be plotted using plot-bamstats\n-# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /Users/anton/galaxy-git/database/files/000/dataset_9.dat /Users/anton/galaxy-git/database/files/000/dataset_10.dat\n+# This file was produced by samtools stats (1.2+htslib-1.2) and can be plotted using plot-bamstats\n+# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_2.dat /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_1.dat\n # CHK, Checksum\t[2]Read Names\t[3]Sequences\t[4]Qualities\n # CHK, CRC32 of reads which passed filtering followed by addition (32bit overflow)\n CHK\t1bd20fd8\t58ad2167\t29883386\n\n-------------------- >> begin captured logging << --------------------\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.web.framework.webapp: INFO: Session authenticated using Galaxy master api key\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/users?key=test_key HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.web.framework.webapp: INFO: Session authenticated using Galaxy master api key\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/users HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.web.framework.webapp: INFO: Session authenticated using Galaxy master api key\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/users/2891970512fa2d5a/api_key HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/histories HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.tools.actions.upload_common: INFO: tool upload1 created job id 1\ngalaxy.tools.execute: DEBUG: Tool [upload1] created job [1] (721.369 ms)\ngalaxy.jobs: DEBUG: (1) Working directory for job is: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1\ngalaxy.jobs.handler: DEBUG: (1) Dispatching to local runner\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/tools HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs: DEBUG: (1) Persisting job destination (destination id: local:///)\ngalaxy.jobs.runners: DEBUG: Job [1] queued (3701.848 ms)\ngalaxy.jobs.handler: INFO: (1) Job dispatched\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs.runners: DEBUG: (1) command is: python /Users/marten/devel/git/galaxy/tools/data_source/upload.py /Users/marten/devel/git/galaxy /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpcOV73W /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpzTLxF3 1:/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1/dataset_1_files:/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_1.dat; return_code=$?; python /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1/set_metadata_aC_Epn.py /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpcOV73W /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1/galaxy.json /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1/metadata_in_HistoryDatasetAssociation_1_2YHWg0,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1/metadata_kwds_HistoryDatasetAssociation_1_xxSWvK,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1/metadata_out_HistoryDatasetAssociation_1_gpHI_B,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1/metadata_results_HistoryDatasetAssociation_1_bOBqlz,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_1.dat,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1/metadata_override_HistoryDatasetAssociation_1_chT4qY; sh -c \"exit $return_code\"\ngalaxy.jobs.runners.local: DEBUG: (1) executing job script: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1/galaxy_1.sh\ngalaxy.jobs: DEBUG: (1) Persisting job destination (destination id: local:///)\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\ngalaxy.jobs.runners.local: DEBUG: execution finished: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/1/galaxy_1.sh\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs: DEBUG: job 1 ended (finish() executed in (3319.682 ms))\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.tools.actions.upload_common: INFO: tool upload1 created job id 2\ngalaxy.tools.execute: DEBUG: Tool [upload1] created job [2] (1436.682 ms)\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/tools HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs: DEBUG: (2) Working directory for job is: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2\ngalaxy.jobs.handler: DEBUG: (2) Dispatching to local runner\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs: DEBUG: (2) Persisting job destination (destination id: local:///)\ngalaxy.jobs.runners: DEBUG: Job [2] queued (2933.918 ms)\ngalaxy.jobs.handler: INFO: (2) Job dispatched\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs.runners: DEBUG: (2) command is: python /Users/marten/devel/git/galaxy/tools/data_source/upload.py /Users/marten/devel/git/galaxy /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpcOV73W /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpQj7Vtj 2:/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2/dataset_2_files:/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_2.dat; return_code=$?; python /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2/set_metadata_EnSKJC.py /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpcOV73W /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2/galaxy.json /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2/metadata_in_HistoryDatasetAssociation_2_EbkTIH,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2/metadata_kwds_HistoryDatasetAssociation_2_A_ZJxl,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2/metadata_out_HistoryDatasetAssociation_2_rYAxQD,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2/metadata_results_HistoryDatasetAssociation_2_1V3qgk,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_2.dat,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2/metadata_override_HistoryDatasetAssociation_2_LI0aVu; sh -c \"exit $return_code\"\ngalaxy.jobs.runners.local: DEBUG: (2) executing job script: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2/galaxy_2.sh\ngalaxy.jobs: DEBUG: (2) Persisting job destination (destination id: local:///)\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs.runners.local: DEBUG: execution finished: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/2/galaxy_2.sh\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs: DEBUG: job 2 ended (finish() executed in (5509.688 ms))\ngalaxy.tools.actions: INFO: Handled output (77.015 ms)\ngalaxy.tools.actions: INFO: Verified access to datasets (55.049 ms)\ngalaxy.tools.execute: DEBUG: Tool [samtools_stats] created job [3] (665.725 ms)\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/tools HTTP/1.1\" 200 None\ngalaxy.jobs: DEBUG: (3) Working directory for job is: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/3\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs.handler: DEBUG: (3) Dispatching to local runner\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/54f2a3a23292eb07?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\ngalaxy.jobs: DEBUG: (3) Persisting job destination (destination id: local:///)\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs.runners: DEBUG: Job [3] queued (1882.584 ms)\ngalaxy.jobs.handler: INFO: (3) Job dispatched\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/54f2a3a23292eb07?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/54f2a3a23292eb07?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\ngalaxy.jobs.runners: DEBUG: (3) command is: samtools --version | head -n 1 | awk '{ print $2 }' > /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/GALAXY_VERSION_STRING_3 2>&1; ln -s \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_2.dat\" && samtools faidx `basename \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_2.dat\"` && samtools stats \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_1.dat\" --coverage 1,1000,1  --GC-depth 20000.0 --insert-size 8000    --most-inserts 0.99 --trim-quality 0 --ref-seq \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_2.dat\" > \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_3.dat\"; return_code=$?; python /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/3/set_metadata_Zey9XK.py /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpcOV73W /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/3/galaxy.json /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/3/metadata_in_HistoryDatasetAssociation_3_c241v6,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/3/metadata_kwds_HistoryDatasetAssociation_3_TUAqpX,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/3/metadata_out_HistoryDatasetAssociation_3_fh3HTD,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/3/metadata_results_HistoryDatasetAssociation_3_y4t5DA,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_3.dat,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/3/metadata_override_HistoryDatasetAssociation_3_3rhcZs; sh -c \"exit $return_code\"\ngalaxy.jobs.runners.local: DEBUG: (3) executing job script: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/3/galaxy_3.sh\ngalaxy.jobs: DEBUG: (3) Persisting job destination (destination id: local:///)\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/54f2a3a23292eb07?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\ngalaxy.jobs.runners.local: DEBUG: execution finished: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/3/galaxy_3.sh\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/54f2a3a23292eb07?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\ngalaxy.jobs: DEBUG: job 3 ended (finish() executed in (2080.708 ms))\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/54f2a3a23292eb07?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/54f2a3a23292eb07?full=true&key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/54f2a3a23292eb07?key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/2891970512fa2d5a/contents/54f2a3a23292eb07/display?raw=true&key=9b3be0c093fde28daf0ffc1ec9b125ca HTTP/1.1\" 200 None\nbase.twilltestcase: DEBUG: keepoutdir: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/jobfiles, ofn: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/jobfiles/samtools_stats_out1.tab\nbase.twilltestcase: DEBUG: ## GALAXY_TEST_SAVE=/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/jobfiles. saved /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/jobfiles/samtools_stats_out1.tab\nbase.twilltestcase: INFO: ## files diff on /Users/marten/devel/git/tools-devteam/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out1.tab and /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpVPmlQNsamtools_stats_out1.tab lines_diff=2, found diff = 4\n--------------------- >> end captured logging << ---------------------\n", "problem_type": "functional.test_toolbox.JobOutputsError"}, "id": "functional.test_toolbox.TestForTool_samtools_stats.test_tool_000000", "has_data": true}, {"data": {"status": "failure", "inputs": {"use_reference|reference_source|reference_source_selector": "history", "input_file": {"src": "hda", "id": "8155e4b4bf1581ff"}, "split_output|split_output_selector": "yes", "split_output|generate_tables": ["sn", "mpc", "gcc"], "use_reference|use_ref_selector": "yes", "use_reference|reference_source|ref_file": {"src": "hda", "id": "7b55dbb89df8f4e5"}}, "output_problems": ["History item  different than expected, difference (using diff):\n( /Users/marten/devel/git/tools-devteam/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2.tab v. /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpYr1M8Csamtools_stats_out2.tab )\n--- local_file\n+++ history_data\n@@ -1,5 +1,5 @@\n-# This file was produced by samtools stats (1.2+htslib-1.2.1) and can be plotted using plot-bamstats\n-# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /Users/anton/galaxy-git/database/files/000/dataset_9.dat /Users/anton/galaxy-git/database/files/000/dataset_10.dat\n+# This file was produced by samtools stats (1.2+htslib-1.2) and can be plotted using plot-bamstats\n+# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_5.dat /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_4.dat\n # CHK, Checksum\t[2]Read Names\t[3]Sequences\t[4]Qualities\n # CHK, CRC32 of reads which passed filtering followed by addition (32bit overflow)\n CHK\t1bd20fd8\t58ad2167\t29883386\n"], "job": {"inputs": {"ref_file": {"src": "hda", "id": "7b55dbb89df8f4e5"}, "input_file": {"src": "hda", "id": "8155e4b4bf1581ff"}}, "update_time": "2015-04-21T21:46:44.081337", "tool_id": "samtools_stats", "outputs": {"__new_primary_file_output|Mismatches per cycle__": {"src": "hda", "id": "a90a30fafe298e1e"}, "__new_primary_file_output|Summary numbers__": {"src": "hda", "id": "b842d972534ccb3e"}, "__new_primary_file_output|ACGT content per cycle__": {"src": "hda", "id": "683bc220e21425bb"}, "output": {"src": "hda", "id": "fa6d20d0fb68383f"}}, "stdout": "sn,mpc,gcc\n", "command_line": "ln -s \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_5.dat\" && samtools faidx `basename \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_5.dat\"` && samtools stats \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_4.dat\" --coverage 1,1000,1  --GC-depth 20000.0 --insert-size 8000    --most-inserts 0.99 --trim-quality 0 --ref-seq \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_5.dat\" > \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" && mkdir split && echo sn,mpc,gcc &&  echo \"# Summary Numbers\\n\"  > \"split/Summary numbers.tab\" && grep -q ^SN  \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" ; if [ $? = 0 ] ; then grep ^SN  \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" | cut -f 2- >> \"split/Summary numbers.tab\"  ; fi &&    echo \"# Columns correspond to qualities, rows to cycles. First column is the cycle number, second is the number of N's and the rest is the number of mismatches\" > \"split/Mismatches per cycle.tab\" && grep -q ^MPC \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" ; if [ $? = 0 ] ; then grep ^MPC \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" | cut -f 2- >> \"split/Mismatches per cycle.tab\" ; fi &&    echo \"# ACGT content per cycle. The columns are: cycle, and A,C,G,T counts (percent)\" > \"split/ACGT content per cycle.tab\" && grep -q ^GCC \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" ; if [ $? = 0 ] ; then grep ^GCC \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" | cut -f 2- >> \"split/ACGT content per cycle.tab\" ; fi &&         true", "exit_code": 0, "state": "ok", "create_time": "2015-04-21T21:46:23.415078", "params": {"coverage_max": "\"1000\"", "gc_depth": "\"20000.0\"", "insert_size": "\"8000\"", "most_inserts": "\"0.99\"", "coverage_step": "\"1\"", "dbkey": "\"hg17\"", "coverage_min": "\"1\"", "read_length": "\"0\"", "trim_quality": "\"0\"", "filter_by_flags": "{\"filter_flags\": \"nofilter\", \"__current_case__\": 1}", "split_output": "{\"generate_tables\": [\"sn\", \"mpc\", \"gcc\"], \"split_output_selector\": \"yes\", \"__current_case__\": 1}", "use_reference": "{\"reference_source\": {\"ref_file\": 5, \"reference_source_selector\": \"history\", \"__current_case__\": 1}, \"use_ref_selector\": \"yes\", \"__current_case__\": 0}", "chromInfo": "\"/Users/marten/devel/git/galaxy/tool-data/shared/ucsc/chrom/hg17.len\"", "remove_dups": "\"False\""}, "stderr": "[fai_load] build FASTA index.\n", "job_metrics": [], "model_class": "Job", "external_id": "64605", "id": "fa6d20d0fb68383f", "user_email": "test@bx.psu.edu"}, "problem_log": "Traceback (most recent call last):\n  File \"/System/Library/Frameworks/Python.framework/Versions/2.7/lib/python2.7/unittest/case.py\", line 331, in run\n    testMethod()\n  File \"/Users/marten/devel/git/galaxy/test/functional/test_toolbox.py\", line 268, in test_tool\n    self.do_it( td )\n  File \"/Users/marten/devel/git/galaxy/test/functional/test_toolbox.py\", line 67, in do_it\n    raise e\nJobOutputsError: History item  different than expected, difference (using diff):\n( /Users/marten/devel/git/tools-devteam/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2.tab v. /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpYr1M8Csamtools_stats_out2.tab )\n--- local_file\n+++ history_data\n@@ -1,5 +1,5 @@\n-# This file was produced by samtools stats (1.2+htslib-1.2.1) and can be plotted using plot-bamstats\n-# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /Users/anton/galaxy-git/database/files/000/dataset_9.dat /Users/anton/galaxy-git/database/files/000/dataset_10.dat\n+# This file was produced by samtools stats (1.2+htslib-1.2) and can be plotted using plot-bamstats\n+# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_5.dat /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_4.dat\n # CHK, Checksum\t[2]Read Names\t[3]Sequences\t[4]Qualities\n # CHK, CRC32 of reads which passed filtering followed by addition (32bit overflow)\n CHK\t1bd20fd8\t58ad2167\t29883386\n\n-------------------- >> begin captured logging << --------------------\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.web.framework.webapp: INFO: Session authenticated using Galaxy master api key\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/users?key=test_key HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.web.framework.webapp: INFO: Session authenticated using Galaxy master api key\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/users/2891970512fa2d5a/api_key HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/histories HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.tools.actions.upload_common: INFO: tool upload1 created job id 4\ngalaxy.tools.execute: DEBUG: Tool [upload1] created job [4] (488.979 ms)\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/tools HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs: DEBUG: (4) Working directory for job is: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs.handler: DEBUG: (4) Dispatching to local runner\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs: DEBUG: (4) Persisting job destination (destination id: local:///)\ngalaxy.jobs.runners: DEBUG: Job [4] queued (3770.035 ms)\ngalaxy.jobs.handler: INFO: (4) Job dispatched\ngalaxy.jobs.runners: DEBUG: (4) command is: python /Users/marten/devel/git/galaxy/tools/data_source/upload.py /Users/marten/devel/git/galaxy /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpcOV73W /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpovV4Rg 4:/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4/dataset_4_files:/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_4.dat; return_code=$?; python /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4/set_metadata_pDUWU1.py /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpcOV73W /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4/galaxy.json /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4/metadata_in_HistoryDatasetAssociation_4_KJFVUK,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4/metadata_kwds_HistoryDatasetAssociation_4_eC_v98,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4/metadata_out_HistoryDatasetAssociation_4_PVfJHp,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4/metadata_results_HistoryDatasetAssociation_4_JUuyR2,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_4.dat,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4/metadata_override_HistoryDatasetAssociation_4_OMLQxN; sh -c \"exit $return_code\"\ngalaxy.jobs.runners.local: DEBUG: (4) executing job script: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4/galaxy_4.sh\ngalaxy.jobs: DEBUG: (4) Persisting job destination (destination id: local:///)\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\ngalaxy.jobs.runners.local: DEBUG: execution finished: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/4/galaxy_4.sh\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs: DEBUG: job 4 ended (finish() executed in (7314.557 ms))\ngalaxy.tools.actions.upload_common: INFO: tool upload1 created job id 5\ngalaxy.tools.execute: DEBUG: Tool [upload1] created job [5] (380.201 ms)\ngalaxy.jobs: DEBUG: (5) Working directory for job is: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/tools HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs.handler: DEBUG: (5) Dispatching to local runner\ngalaxy.jobs: DEBUG: (5) Persisting job destination (destination id: local:///)\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs.runners: DEBUG: Job [5] queued (2761.054 ms)\ngalaxy.jobs.handler: INFO: (5) Job dispatched\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs.runners: DEBUG: (5) command is: python /Users/marten/devel/git/galaxy/tools/data_source/upload.py /Users/marten/devel/git/galaxy /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpcOV73W /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpx_FyDt 5:/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5/dataset_5_files:/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_5.dat; return_code=$?; python /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5/set_metadata_ErwZWr.py /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpcOV73W /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5/galaxy.json /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5/metadata_in_HistoryDatasetAssociation_5_bf6GWj,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5/metadata_kwds_HistoryDatasetAssociation_5_qdTYxt,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5/metadata_out_HistoryDatasetAssociation_5_nILjxU,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5/metadata_results_HistoryDatasetAssociation_5_nF9gHk,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_5.dat,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5/metadata_override_HistoryDatasetAssociation_5_xELsea; sh -c \"exit $return_code\"\ngalaxy.jobs.runners.local: DEBUG: (5) executing job script: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5/galaxy_5.sh\ngalaxy.jobs: DEBUG: (5) Persisting job destination (destination id: local:///)\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\ngalaxy.jobs.runners.local: DEBUG: execution finished: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/5/galaxy_5.sh\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.tools.actions: INFO: Handled output (619.072 ms)\nsqlalchemy.pool.NullPool: ERROR: Exception during reset or similar\nTraceback (most recent call last):\n  File \"build/bdist.macosx-10.6-intel/egg/sqlalchemy/pool.py\", line 567, in _finalize_fairy\n    fairy._reset(pool)\n  File \"build/bdist.macosx-10.6-intel/egg/sqlalchemy/pool.py\", line 701, in _reset\n    pool._dialect.do_rollback(self)\n  File \"build/bdist.macosx-10.6-intel/egg/sqlalchemy/engine/default.py\", line 412, in do_rollback\n    dbapi_connection.rollback()\nProgrammingError: SQLite objects created in a thread can only be used in that same thread.The object was created in thread id 4568608768 and this is thread id 4471201792\nsqlalchemy.pool.NullPool: ERROR: Exception closing connection <pysqlite2.dbapi2.Connection object at 0x111207ce0>\nTraceback (most recent call last):\n  File \"build/bdist.macosx-10.6-intel/egg/sqlalchemy/pool.py\", line 251, in _close_connection\n    self._dialect.do_close(connection)\n  File \"build/bdist.macosx-10.6-intel/egg/sqlalchemy/engine/default.py\", line 418, in do_close\n    dbapi_connection.close()\nProgrammingError: SQLite objects created in a thread can only be used in that same thread.The object was created in thread id 4568608768 and this is thread id 4471201792\ngalaxy.jobs: DEBUG: job 5 ended (finish() executed in (6572.427 ms))\ngalaxy.tools.actions: INFO: Verified access to datasets (340.159 ms)\ngalaxy.tools.execute: DEBUG: Tool [samtools_stats] created job [6] (4823.769 ms)\ngalaxy.jobs: DEBUG: (6) Working directory for job is: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/6\ngalaxy.jobs.handler: DEBUG: (6) Dispatching to local runner\nrequests.packages.urllib3.connectionpool: DEBUG: \"POST /api/tools HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\ngalaxy.jobs: DEBUG: (6) Persisting job destination (destination id: local:///)\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/fa6d20d0fb68383f?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\ngalaxy.jobs.runners: DEBUG: Job [6] queued (3581.802 ms)\ngalaxy.jobs.handler: INFO: (6) Job dispatched\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/fa6d20d0fb68383f?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/fa6d20d0fb68383f?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/fa6d20d0fb68383f?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\ngalaxy.jobs.runners: DEBUG: (6) command is: samtools --version | head -n 1 | awk '{ print $2 }' > /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/GALAXY_VERSION_STRING_6 2>&1; ln -s \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_5.dat\" && samtools faidx `basename \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_5.dat\"` && samtools stats \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_4.dat\" --coverage 1,1000,1  --GC-depth 20000.0 --insert-size 8000    --most-inserts 0.99 --trim-quality 0 --ref-seq \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_5.dat\" > \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" && mkdir split && echo sn,mpc,gcc &&  echo \"# Summary Numbers\\n\"  > \"split/Summary numbers.tab\" && grep -q ^SN  \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" ; if [ $? = 0 ] ; then grep ^SN  \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" | cut -f 2- >> \"split/Summary numbers.tab\"  ; fi &&    echo \"# Columns correspond to qualities, rows to cycles. First column is the cycle number, second is the number of N's and the rest is the number of mismatches\" > \"split/Mismatches per cycle.tab\" && grep -q ^MPC \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" ; if [ $? = 0 ] ; then grep ^MPC \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" | cut -f 2- >> \"split/Mismatches per cycle.tab\" ; fi &&    echo \"# ACGT content per cycle. The columns are: cycle, and A,C,G,T counts (percent)\" > \"split/ACGT content per cycle.tab\" && grep -q ^GCC \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" ; if [ $? = 0 ] ; then grep ^GCC \"/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat\" | cut -f 2- >> \"split/ACGT content per cycle.tab\" ; fi &&         true; return_code=$?; python /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/6/set_metadata_LSTtun.py /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpcOV73W /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/6/galaxy.json /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/6/metadata_in_HistoryDatasetAssociation_6_uh9zmy,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/6/metadata_kwds_HistoryDatasetAssociation_6_6GLt0N,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/6/metadata_out_HistoryDatasetAssociation_6_OK7DHx,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/6/metadata_results_HistoryDatasetAssociation_6_GeXlkW,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/files/000/dataset_6.dat,/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/6/metadata_override_HistoryDatasetAssociation_6_ZyuaJN; sh -c \"exit $return_code\"\ngalaxy.jobs.runners.local: DEBUG: (6) executing job script: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/6/galaxy_6.sh\ngalaxy.jobs: DEBUG: (6) Persisting job destination (destination id: local:///)\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/fa6d20d0fb68383f?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\ngalaxy.jobs.runners.local: DEBUG: execution finished: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/job_working_directory/000/6/galaxy_6.sh\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/fa6d20d0fb68383f?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\ngalaxy.jobs: DEBUG: job 6 ended (finish() executed in (6680.679 ms))\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/fa6d20d0fb68383f?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/fa6d20d0fb68383f?full=true&key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/jobs/fa6d20d0fb68383f?key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nrequests.packages.urllib3.connectionpool: INFO: Starting new HTTP connection (1): localhost\nrequests.packages.urllib3.connectionpool: DEBUG: \"GET /api/histories/5729865256bc2525/contents/fa6d20d0fb68383f/display?raw=true&key=73391df1185a0911acd55c3f7fae680e HTTP/1.1\" 200 None\nbase.twilltestcase: DEBUG: keepoutdir: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/jobfiles, ofn: /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/jobfiles/samtools_stats_out2.tab\nbase.twilltestcase: DEBUG: ## GALAXY_TEST_SAVE=/var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/jobfiles. saved /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/jobfiles/samtools_stats_out2.tab\nbase.twilltestcase: INFO: ## files diff on /Users/marten/devel/git/tools-devteam/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2.tab and /var/folders/2z/ntdb9g2n7tq6_bcq4dj9hj9r0000gn/T/tmp69oNhW/tmp/tmpYr1M8Csamtools_stats_out2.tab lines_diff=2, found diff = 4\n--------------------- >> end captured logging << ---------------------\n", "problem_type": "functional.test_toolbox.JobOutputsError"}, "id": "functional.test_toolbox.TestForTool_samtools_stats.test_tool_000001", "has_data": true}], "version": "0.1", "summary": {"num_skips": 0, "num_errors": 0, "num_failures": 2, "num_tests": 2}};
-        renderTestResults(test_data);
-      }
-    </script>
-  </body>
-</html>

From 5ce99d2a69585902788fd9d462488cbe118949d6 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Bj=C3=B6rn=20Gr=C3=BCning?= <bjoern@gruenings.eu>
Date: Tue, 25 Apr 2017 10:01:13 +0200
Subject: [PATCH 33/40] Fix more tests

---
 .../samtools_flagstat/test-data/samtools_flagstat_out1.txt    | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/tool_collections/samtools/samtools_flagstat/test-data/samtools_flagstat_out1.txt b/tool_collections/samtools/samtools_flagstat/test-data/samtools_flagstat_out1.txt
index 02f581e8960..740dc23f4c5 100644
--- a/tool_collections/samtools/samtools_flagstat/test-data/samtools_flagstat_out1.txt
+++ b/tool_collections/samtools/samtools_flagstat/test-data/samtools_flagstat_out1.txt
@@ -2,12 +2,12 @@
 0 + 0 secondary
 0 + 0 supplementary
 0 + 0 duplicates
-25 + 0 mapped (12.50%:-nan%)
+25 + 0 mapped (12.50% : N/A)
 200 + 0 paired in sequencing
 100 + 0 read1
 100 + 0 read2
 0 + 0 properly paired (0.00%:-nan%)
 0 + 0 with itself and mate mapped
-25 + 0 singletons (12.50%:-nan%)
+25 + 0 singletons (12.50% : N/A)
 0 + 0 with mate mapped to a different chr
 0 + 0 with mate mapped to a different chr (mapQ>=5)

From 96d9878f547465f1f3dce670e900ef3f29318833 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Bj=C3=B6rn=20Gr=C3=BCning?= <bjoern@gruenings.eu>
Date: Tue, 25 Apr 2017 10:05:24 +0200
Subject: [PATCH 34/40] test fixes

---
 .../samtools_mpileup/test-data/samtools_mpileup_out_2.vcf     | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_2.vcf b/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_2.vcf
index e9fdae00908..5a1dd7641ba 100644
--- a/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_2.vcf
+++ b/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_2.vcf
@@ -4,7 +4,7 @@
 ##samtoolsCommand=samtools mpileup --VCF --uncompressed -f /tmp/tmpId8vOP/tmp3bubIE/database/files/000/dataset_735.dat -g -e 20 -h 100 -L 250 -m 1 --open-prob 40 -F 0.002 -e 40 --output /tmp/tmpId8vOP/tmp3bubIE/database/files/000/dataset_736.dat /tmp/tmpId8vOP/tmp3bubIE/database/files/000/dataset_734.dat
 ##reference=file:///tmp/tmpId8vOP/tmp3bubIE/database/files/000/dataset_735.dat
 ##contig=<ID=phiX174,length=5386>
-##ALT=<ID=X,Description="Represents allele(s) other than observed.">
+##ALT=<ID=*,Description="Represents allele(s) other than observed.">
 ##INFO=<ID=INDEL,Number=0,Type=Flag,Description="Indicates that the variant is an INDEL.">
 ##INFO=<ID=IDV,Number=1,Type=Integer,Description="Maximum number of reads supporting an indel">
 ##INFO=<ID=IMF,Number=1,Type=Float,Description="Maximum fraction of reads supporting an indel">
@@ -19,4 +19,4 @@
 ##INFO=<ID=I16,Number=16,Type=Float,Description="Auxiliary tag used for calling, see description of bcf_callret1_t in bam2bcf.h">
 ##INFO=<ID=QS,Number=R,Type=Float,Description="Auxiliary tag used for calling">
 ##FORMAT=<ID=PL,Number=G,Type=Integer,Description="List of Phred-scaled genotype likelihoods">
-#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	/tmp/tmpId8vOP/tmp3bubIE/database/files/000/dataset_734.dat
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	localbam_0.bam

From b7585950e3a1f0be5ea9b37e3768f508aad2093d Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Bj=C3=B6rn=20Gr=C3=BCning?= <bjoern@gruenings.eu>
Date: Tue, 25 Apr 2017 10:35:54 +0200
Subject: [PATCH 35/40] Change test data

---
 .../samtools_flagstat/test-data/samtools_flagstat_out1.txt      | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/tool_collections/samtools/samtools_flagstat/test-data/samtools_flagstat_out1.txt b/tool_collections/samtools/samtools_flagstat/test-data/samtools_flagstat_out1.txt
index 740dc23f4c5..f423a1614d9 100644
--- a/tool_collections/samtools/samtools_flagstat/test-data/samtools_flagstat_out1.txt
+++ b/tool_collections/samtools/samtools_flagstat/test-data/samtools_flagstat_out1.txt
@@ -6,7 +6,7 @@
 200 + 0 paired in sequencing
 100 + 0 read1
 100 + 0 read2
-0 + 0 properly paired (0.00%:-nan%)
+0 + 0 properly paired (0.00% : N/A)
 0 + 0 with itself and mate mapped
 25 + 0 singletons (12.50% : N/A)
 0 + 0 with mate mapped to a different chr

From 2f64c9df768e0dea83d05ebb2cd5d0d313d55ae0 Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Wed, 26 Apr 2017 10:06:44 +0200
Subject: [PATCH 36/40] removal of not used test files

---
 .../mpileup/samtools_mpileup_out_1.pileup     |  43 ------------------
 .../mpileup/samtools_mpileup_out_2.bcf        | Bin 426 -> 0 bytes
 2 files changed, 43 deletions(-)
 delete mode 100644 tool_collections/samtools/samtools_mpileup/test-data/samtools/mpileup/samtools_mpileup_out_1.pileup
 delete mode 100644 tool_collections/samtools/samtools_mpileup/test-data/samtools/mpileup/samtools_mpileup_out_2.bcf

diff --git a/tool_collections/samtools/samtools_mpileup/test-data/samtools/mpileup/samtools_mpileup_out_1.pileup b/tool_collections/samtools/samtools_mpileup/test-data/samtools/mpileup/samtools_mpileup_out_1.pileup
deleted file mode 100644
index c41b10ab290..00000000000
--- a/tool_collections/samtools/samtools_mpileup/test-data/samtools/mpileup/samtools_mpileup_out_1.pileup
+++ /dev/null
@@ -1,43 +0,0 @@
-phiX174	1411	A	1	^P.	$
-phiX174	1412	G	3	.^D.^F.	"$$
-phiX174	1413	C	5	...^D.^F.	"""$$
-phiX174	1414	G	6	.....^F.	#####$
-phiX174	1415	C	7	......^F.	%%%%%%&
-phiX174	1416	C	8	.......^F.	$$$$$$$$
-phiX174	1417	G	9	........^F.	"#######$
-phiX174	1418	T	10	.........^F.	"""""""""$
-phiX174	1419	G	10	..........	"""""'&'%$
-phiX174	1420	G	10	..........	""""""""""
-phiX174	1421	A	10	..........	""""""""""
-phiX174	1422	T	10	..........	""""""""""
-phiX174	1423	G	10	..........	"""""""""#
-phiX174	1424	C	10	..A.AAAAAA	%"""""""""
-phiX174	1425	C	10	..........	$$$"""""""
-phiX174	1426	T	10	..........	#####"""""
-phiX174	1427	G	10	..........	######""""
-phiX174	1428	A	10	..........	""""""""""
-phiX174	1429	C	10	..........	((((((&(""
-phiX174	1430	C	10	..........	$$$$$$$$$"
-phiX174	1431	G	10	..........	##########
-phiX174	1432	T	10	..........	""""""""""
-phiX174	1433	A	10	..........	##########
-phiX174	1434	C	10	..........	((((((&(%$
-phiX174	1435	C	10	..........	$$$$$$$$$$
-phiX174	1436	G	10	..........	##########
-phiX174	1437	A	10	..........	"""""""""!
-phiX174	1438	G	10	..........	"""""####!
-phiX174	1439	G	10	..........	"""""""""!
-phiX174	1440	C	10	..........	"""""""""!
-phiX174	1441	T	10	..........	""""""""#!
-phiX174	1442	A	10	..........	$$$%%%&&%!
-phiX174	1443	A	10	.-1C.-1C..-1C......	"""""""""!
-phiX174	1444	C	10	**.*......	&%"!"""""!
-phiX174	1445	C	10	..........	&%&!%%%&%!
-phiX174	1446	C	10	..........	"""!"""""!
-phiX174	1447	T	10	.$..$.......	#"#!"""""!
-phiX174	1448	A	8	.$..$.....	#!#%%$$!
-phiX174	1449	A	6	.$.$....	!""""!
-phiX174	1450	T	4	.$...	"""!
-phiX174	1451	G	3	.$..	#"!
-phiX174	1452	A	2	.$.	"!
-phiX174	1453	G	1	.$	!
diff --git a/tool_collections/samtools/samtools_mpileup/test-data/samtools/mpileup/samtools_mpileup_out_2.bcf b/tool_collections/samtools/samtools_mpileup/test-data/samtools/mpileup/samtools_mpileup_out_2.bcf
deleted file mode 100644
index 3017c84a089c8bea7381e8a16e836d416b1e5e0d..0000000000000000000000000000000000000000
GIT binary patch
literal 0
HcmV?d00001

literal 426
zcmb2|=3rp}f&Xj_PR>jWX$-|hWyuc{5)w2{oaiwyF;5g=+axP`M`&}fsDl&N!Ooqm
zmMeKyIV7e&eRSl`q(6V=Jlb$&(W5heY@(TMoo$`DS$mbdxs|n*y@P%A*cetEKCT)7
zG+iFe8a2kX*P^|d0tMJVRDQheG3V}C(@3)$A}%iuvUl3`N6nmCemXieZubvGS4-~C
z1|FOLoIh0g$MExfW@{Iht0J=f)jO1SscKDeR@bns%ebU&uN6Pp*0fJV_mSpAEB8-g
zCp9KouM~>!yyHDJW$KCMmKcqRZCCvKI&GqkSjSAQIO4X_WM`1iDjUZs>FZoPr;2;%
z>3X>5u8#S1{gb`w4$YX>XJ;fPCnmH{U&=i5Qmf0AW|u4NJhcobQ#k_T6nE}%OfzX(
zV%_${s8J)>W0$dq-unqrb_%D|Rqk3oxmxRZDb`_%>ZA4hn_70aX-J>w*mkLD#ogq6
xsxG^y{Vh;=_dKTak5brG`<1fcoo|+Snaut!*Y(VP|9)=FV3KBF21gEv003J)snh@f


From c339ded8a9e3461cb792bb64b631ae322f51190b Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Wed, 26 Apr 2017 14:11:07 +0200
Subject: [PATCH 37/40] upgraded mpileup tests

---
 .../samtools_mpileup/samtools_mpileup.xml     | 11 +++
 .../test-data/samtools_mpileup_out_1.pileup   | 86 +++++++++----------
 .../test-data/samtools_mpileup_out_3.pileup   | 43 ++++++++++
 3 files changed, 97 insertions(+), 43 deletions(-)
 create mode 100644 tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_3.pileup

diff --git a/tool_collections/samtools/samtools_mpileup/samtools_mpileup.xml b/tool_collections/samtools/samtools_mpileup/samtools_mpileup.xml
index 6c5c586414b..9d703fd5a8f 100644
--- a/tool_collections/samtools/samtools_mpileup/samtools_mpileup.xml
+++ b/tool_collections/samtools/samtools_mpileup/samtools_mpileup.xml
@@ -310,6 +310,17 @@ ${pasted_data}
             <output file="samtools_mpileup_out_2.vcf" ftype="vcf" lines_diff="8" name="output_mpileup" />
             <output file="samtools_mpileup_out_2.log.txt" name="output_log" />
         </test>
+        <test>
+            <param name="reference_source_selector" value="history" />
+            <param ftype="fasta" name="ref_file" value="phiX.fasta" />
+            <param ftype="bam" name="input_bam" value="samtools_mpileup_in_1.bam" />
+            <param name="genotype_likelihood_computation_type_selector" value="do_not_perform_genotype_likelihood_computation" />
+            <param name="advanced_options_selector" value="advanced" />
+            <param name="minimum_base_quality" value="0" /><!-- most reads have ultra low quality resuling in empty columns -->
+            <param name="base_position_on_reads" value="true" />
+            <param name="output_mapping_quality" value="true" />
+            <output file="samtools_mpileup_out_3.pileup" name="output_mpileup" />
+        </test>
     </tests>
     <help>
 <![CDATA[
diff --git a/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_1.pileup b/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_1.pileup
index b75cb580982..fad9cab45f6 100644
--- a/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_1.pileup
+++ b/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_1.pileup
@@ -1,43 +1,43 @@
-phiX174	1411	A	0				1
-phiX174	1412	G	0				2,1,1
-phiX174	1413	C	0				3,2,2,1,1
-phiX174	1414	G	0				4,3,3,2,2,1
-phiX174	1415	C	0				5,4,4,3,3,2,1
-phiX174	1416	C	0				6,5,5,4,4,3,2,1
-phiX174	1417	G	0				7,6,6,5,5,4,3,2,1
-phiX174	1418	T	0				8,7,7,6,6,5,4,3,2,1
-phiX174	1419	G	0				9,8,8,7,7,6,5,4,3,2
-phiX174	1420	G	0				10,9,9,8,8,7,6,5,4,3
-phiX174	1421	A	0				11,10,10,9,9,8,7,6,5,4
-phiX174	1422	T	0				12,11,11,10,10,9,8,7,6,5
-phiX174	1423	G	0				13,12,12,11,11,10,9,8,7,6
-phiX174	1424	C	0				14,13,13,12,12,11,10,9,8,7
-phiX174	1425	C	0				15,14,14,13,13,12,11,10,9,8
-phiX174	1426	T	0				16,15,15,14,14,13,12,11,10,9
-phiX174	1427	G	0				17,16,16,15,15,14,13,12,11,10
-phiX174	1428	A	0				18,17,17,16,16,15,14,13,12,11
-phiX174	1429	C	0				19,18,18,17,17,16,15,14,13,12
-phiX174	1430	C	0				20,19,19,18,18,17,16,15,14,13
-phiX174	1431	G	0				21,20,20,19,19,18,17,16,15,14
-phiX174	1432	T	0				22,21,21,20,20,19,18,17,16,15
-phiX174	1433	A	0				23,22,22,21,21,20,19,18,17,16
-phiX174	1434	C	0				24,23,23,22,22,21,20,19,18,17
-phiX174	1435	C	0				25,24,24,23,23,22,21,20,19,18
-phiX174	1436	G	0				26,25,25,24,24,23,22,21,20,19
-phiX174	1437	A	0				27,26,26,25,25,24,23,22,21,20
-phiX174	1438	G	0				28,27,27,26,26,25,24,23,22,21
-phiX174	1439	G	0				29,28,28,27,27,26,25,24,23,22
-phiX174	1440	C	0				30,29,29,28,28,27,26,25,24,23
-phiX174	1441	T	0				31,30,30,29,29,28,27,26,25,24
-phiX174	1442	A	0				32,31,31,30,30,29,28,27,26,25
-phiX174	1443	A	0				33,32,32,31,31,30,29,28,27,26
-phiX174	1444	C	0				34,33,33,32,32,31,30,29,28,27
-phiX174	1445	C	0				34,33,34,32,33,32,31,30,29,28
-phiX174	1446	C	0				35,34,35,33,34,33,32,31,30,29
-phiX174	1447	T	0				36,35,36,34,35,34,33,32,31,30
-phiX174	1448	A	0				36,35,36,35,34,33,32,31
-phiX174	1449	A	0				36,36,35,34,33,32
-phiX174	1450	T	0				36,35,34,33
-phiX174	1451	G	0				36,35,34
-phiX174	1452	A	0				36,35
-phiX174	1453	G	0				36
+phiX174	1411	A	0				
+phiX174	1412	G	0				
+phiX174	1413	C	0				
+phiX174	1414	G	0				
+phiX174	1415	C	0				
+phiX174	1416	C	0				
+phiX174	1417	G	0				
+phiX174	1418	T	0				
+phiX174	1419	G	0				
+phiX174	1420	G	0				
+phiX174	1421	A	0				
+phiX174	1422	T	0				
+phiX174	1423	G	0				
+phiX174	1424	C	0				
+phiX174	1425	C	0				
+phiX174	1426	T	0				
+phiX174	1427	G	0				
+phiX174	1428	A	0				
+phiX174	1429	C	0				
+phiX174	1430	C	0				
+phiX174	1431	G	0				
+phiX174	1432	T	0				
+phiX174	1433	A	0				
+phiX174	1434	C	0				
+phiX174	1435	C	0				
+phiX174	1436	G	0				
+phiX174	1437	A	0				
+phiX174	1438	G	0				
+phiX174	1439	G	0				
+phiX174	1440	C	0				
+phiX174	1441	T	0				
+phiX174	1442	A	0				
+phiX174	1443	A	0				
+phiX174	1444	C	0				
+phiX174	1445	C	0				
+phiX174	1446	C	0				
+phiX174	1447	T	0				
+phiX174	1448	A	0				
+phiX174	1449	A	0				
+phiX174	1450	T	0				
+phiX174	1451	G	0				
+phiX174	1452	A	0				
+phiX174	1453	G	0				
diff --git a/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_3.pileup b/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_3.pileup
new file mode 100644
index 00000000000..f494a27538d
--- /dev/null
+++ b/tool_collections/samtools/samtools_mpileup/test-data/samtools_mpileup_out_3.pileup
@@ -0,0 +1,43 @@
+phiX174	1411	A	1	^P.	$	P	1
+phiX174	1412	G	3	.^D.^F.	"$$	PDF	2,1,1
+phiX174	1413	C	5	...^D.^F.	"""$$	PDFDF	3,2,2,1,1
+phiX174	1414	G	6	.....^F.	#####$	PDFDFF	4,3,3,2,2,1
+phiX174	1415	C	7	......^F.	%%%%%%&	PDFDFFF	5,4,4,3,3,2,1
+phiX174	1416	C	8	.......^F.	$$$$$$$$	PDFDFFFF	6,5,5,4,4,3,2,1
+phiX174	1417	G	9	........^F.	"#######$	PDFDFFFFF	7,6,6,5,5,4,3,2,1
+phiX174	1418	T	10	.........^F.	"""""""""$	PDFDFFFFFF	8,7,7,6,6,5,4,3,2,1
+phiX174	1419	G	10	..........	"""""'&'%$	PDFDFFFFFF	9,8,8,7,7,6,5,4,3,2
+phiX174	1420	G	10	..........	""""""""""	PDFDFFFFFF	10,9,9,8,8,7,6,5,4,3
+phiX174	1421	A	10	..........	""""""""""	PDFDFFFFFF	11,10,10,9,9,8,7,6,5,4
+phiX174	1422	T	10	..........	""""""""""	PDFDFFFFFF	12,11,11,10,10,9,8,7,6,5
+phiX174	1423	G	10	..........	"""""""""#	PDFDFFFFFF	13,12,12,11,11,10,9,8,7,6
+phiX174	1424	C	10	..A.AAAAAA	%"""""""""	PDFDFFFFFF	14,13,13,12,12,11,10,9,8,7
+phiX174	1425	C	10	..........	$$$"""""""	PDFDFFFFFF	15,14,14,13,13,12,11,10,9,8
+phiX174	1426	T	10	..........	#####"""""	PDFDFFFFFF	16,15,15,14,14,13,12,11,10,9
+phiX174	1427	G	10	..........	######""""	PDFDFFFFFF	17,16,16,15,15,14,13,12,11,10
+phiX174	1428	A	10	..........	""""""""""	PDFDFFFFFF	18,17,17,16,16,15,14,13,12,11
+phiX174	1429	C	10	..........	((((((&(""	PDFDFFFFFF	19,18,18,17,17,16,15,14,13,12
+phiX174	1430	C	10	..........	$$$$$$$$$"	PDFDFFFFFF	20,19,19,18,18,17,16,15,14,13
+phiX174	1431	G	10	..........	##########	PDFDFFFFFF	21,20,20,19,19,18,17,16,15,14
+phiX174	1432	T	10	..........	""""""""""	PDFDFFFFFF	22,21,21,20,20,19,18,17,16,15
+phiX174	1433	A	10	..........	##########	PDFDFFFFFF	23,22,22,21,21,20,19,18,17,16
+phiX174	1434	C	10	..........	((((((&(%$	PDFDFFFFFF	24,23,23,22,22,21,20,19,18,17
+phiX174	1435	C	10	..........	$$$$$$$$$$	PDFDFFFFFF	25,24,24,23,23,22,21,20,19,18
+phiX174	1436	G	10	..........	##########	PDFDFFFFFF	26,25,25,24,24,23,22,21,20,19
+phiX174	1437	A	10	..........	"""""""""!	PDFDFFFFFF	27,26,26,25,25,24,23,22,21,20
+phiX174	1438	G	10	..........	"""""####!	PDFDFFFFFF	28,27,27,26,26,25,24,23,22,21
+phiX174	1439	G	10	..........	"""""""""!	PDFDFFFFFF	29,28,28,27,27,26,25,24,23,22
+phiX174	1440	C	10	..........	"""""""""!	PDFDFFFFFF	30,29,29,28,28,27,26,25,24,23
+phiX174	1441	T	10	..........	""""""""#!	PDFDFFFFFF	31,30,30,29,29,28,27,26,25,24
+phiX174	1442	A	10	..........	$$$%%%&&%!	PDFDFFFFFF	32,31,31,30,30,29,28,27,26,25
+phiX174	1443	A	10	.-1C.-1C..-1C......	"""""""""!	PDFDFFFFFF	33,32,32,31,31,30,29,28,27,26
+phiX174	1444	C	10	**.*......	!!"!"""""!	PDFDFFFFFF	34,33,33,32,32,31,30,29,28,27
+phiX174	1445	C	10	..........	!!&!%%%&%!	PDFDFFFFFF	34,33,34,32,33,32,31,30,29,28
+phiX174	1446	C	10	..........	!!"!"""""!	PDFDFFFFFF	35,34,35,33,34,33,32,31,30,29
+phiX174	1447	T	10	.$..$.......	!!#!"""""!	PDFDFFFFFF	36,35,36,34,35,34,33,32,31,30
+phiX174	1448	A	8	.$..$.....	!!#%%$$!	DDFFFFFF	36,35,36,35,34,33,32,31
+phiX174	1449	A	6	.$.$....	!""""!	DFFFFF	36,36,35,34,33,32
+phiX174	1450	T	4	.$...	"""!	FFFF	36,35,34,33
+phiX174	1451	G	3	.$..	#"!	FFF	36,35,34
+phiX174	1452	A	2	.$.	"!	FF	36,35
+phiX174	1453	G	1	.$	!	F	36

From 8e28b3013c18e068eb8e11c6f345f3d88ec4b503 Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Wed, 26 Apr 2017 14:42:13 +0200
Subject: [PATCH 38/40] samtools reheader: allow testing with 1 line difference
 to correct for addtional reheader-header

---
 .../samtools/samtools_reheader/samtools_reheader.xml            | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/tool_collections/samtools/samtools_reheader/samtools_reheader.xml b/tool_collections/samtools/samtools_reheader/samtools_reheader.xml
index bc732fb529b..31d5fafb203 100644
--- a/tool_collections/samtools/samtools_reheader/samtools_reheader.xml
+++ b/tool_collections/samtools/samtools_reheader/samtools_reheader.xml
@@ -23,7 +23,7 @@
         <test>
             <param name="input_header" ftype="bam" value="reheader_input_header.bam"/>
             <param name="input_file" ftype="bam" value="reheader_input_file.bam"/>
-            <output name="output" ftype="bam" file="reheader_out.bam"/>
+            <output name="output" ftype="bam" file="reheader_out.bam" lines_diff="1"/><!-- samtools adds reheader line -->
         </test>
     </tests>
     <help>

From 9cf113c01c7125bdc015c63c90c4f42c9ece6d0b Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Fri, 28 Apr 2017 15:09:19 +0200
Subject: [PATCH 39/40] samtools stats also reports stats on "N" and "0"

---
 .../samtools_stats/samtools_stats.xml         |   4 +-
 .../test-data/samtools_stats_out1.tab         | 509 +++++++++---------
 .../test-data/samtools_stats_out2.tab         | 509 +++++++++---------
 3 files changed, 512 insertions(+), 510 deletions(-)

diff --git a/tool_collections/samtools/samtools_stats/samtools_stats.xml b/tool_collections/samtools/samtools_stats/samtools_stats.xml
index 018bf49762b..bfb3ecb2a17 100644
--- a/tool_collections/samtools/samtools_stats/samtools_stats.xml
+++ b/tool_collections/samtools/samtools_stats/samtools_stats.xml
@@ -1,8 +1,8 @@
 <tool id="samtools_stats" name="Stats" version="2.0">
     <description>generate statistics for BAM dataset</description>
     <macros>
-    <import>macros.xml</import>
-  </macros>
+        <import>macros.xml</import>
+    </macros>
     <expand macro="requirements"/>
     <expand macro="stdio"/>
     <expand macro="version_command"/>
diff --git a/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out1.tab b/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out1.tab
index 7867a751100..059c9f042d0 100644
--- a/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out1.tab
+++ b/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out1.tab
@@ -1,5 +1,6 @@
-# This file was produced by samtools stats (1.2+htslib-1.2.1) and can be plotted using plot-bamstats
-# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /Users/anton/galaxy-git/database/files/000/dataset_9.dat /Users/anton/galaxy-git/database/files/000/dataset_10.dat
+# This file was produced by samtools stats (1.3.1+htslib-1.3.1) and can be plotted using plot-bamstats
+# This file contains statistics for all reads.
+# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /tmp/tmpO1aTrb/files/000/dataset_2.dat /tmp/tmpO1aTrb/files/000/dataset_1.dat
 # CHK, Checksum	[2]Read Names	[3]Sequences	[4]Qualities
 # CHK, CRC32 of reads which passed filtering followed by addition (32bit overflow)
 CHK	1bd20fd8	58ad2167	29883386
@@ -836,258 +837,258 @@ GCL	51.51	4
 GCL	52.01	1
 GCL	52.51	0
 GCL	53.02	1
-# ACGT content per cycle. Use `grep ^GCC | cut -f 2-` to extract this part. The columns are: cycle, and A,C,G,T counts [%]
-GCC	1	21.50	29.50	33.50	15.50
-GCC	2	30.00	16.00	11.00	43.00
-GCC	3	33.00	25.00	9.00	33.00
-GCC	4	17.00	29.00	13.00	41.00
-GCC	5	37.00	22.00	12.00	29.00
-GCC	6	36.00	26.00	17.00	21.00
-GCC	7	29.50	13.50	31.50	25.50
-GCC	8	50.50	14.50	19.50	15.50
-GCC	9	17.00	33.00	18.00	32.00
-GCC	10	37.00	14.00	21.00	28.00
-GCC	11	20.50	14.50	29.50	35.50
-GCC	12	30.00	24.00	22.00	24.00
-GCC	13	27.00	15.00	21.00	37.00
-GCC	14	24.00	22.00	26.00	28.00
-GCC	15	25.50	20.50	24.50	29.50
-GCC	16	31.00	15.00	20.00	34.00
-GCC	17	28.00	25.00	14.00	33.00
-GCC	18	30.50	28.50	19.50	21.50
-GCC	19	29.00	26.00	21.00	24.00
-GCC	20	22.50	23.50	17.50	36.50
-GCC	21	35.50	17.50	19.50	27.50
-GCC	22	37.50	28.50	15.50	18.50
-GCC	23	31.00	19.00	13.00	37.00
-GCC	24	37.00	12.00	22.00	29.00
-GCC	25	35.50	22.50	17.50	24.50
-GCC	26	33.50	18.50	15.50	32.50
-GCC	27	34.50	14.50	25.50	25.50
-GCC	28	31.00	14.00	24.00	31.00
-GCC	29	30.00	27.00	24.00	19.00
-GCC	30	31.00	20.00	14.00	35.00
-GCC	31	33.50	29.50	13.50	23.50
-GCC	32	42.50	20.50	19.50	17.50
-GCC	33	25.50	23.50	14.50	36.50
-GCC	34	39.50	16.50	20.50	23.50
-GCC	35	32.50	23.50	21.50	22.50
-GCC	36	42.00	25.00	16.00	17.00
-GCC	37	38.00	17.00	19.00	26.00
-GCC	38	24.00	26.00	25.00	25.00
-GCC	39	22.50	41.50	18.50	17.50
-GCC	40	32.00	16.00	21.00	31.00
-GCC	41	33.00	28.00	19.00	20.00
-GCC	42	30.50	25.50	19.50	24.50
-GCC	43	35.00	29.00	15.00	21.00
-GCC	44	20.00	27.00	22.00	31.00
-GCC	45	40.50	21.50	21.50	16.50
-GCC	46	26.50	20.50	22.50	30.50
-GCC	47	38.50	29.50	16.50	15.50
-GCC	48	27.50	24.50	17.50	30.50
-GCC	49	28.50	32.50	10.50	28.50
-GCC	50	46.50	20.50	9.50	23.50
-GCC	51	34.50	28.50	13.50	23.50
-GCC	52	41.50	23.50	20.50	14.50
-GCC	53	20.00	28.00	26.00	26.00
-GCC	54	31.50	18.50	24.50	25.50
-GCC	55	30.50	22.50	16.50	30.50
-GCC	56	33.50	22.50	13.50	30.50
-GCC	57	23.00	24.00	23.00	30.00
-GCC	58	25.00	37.00	19.00	19.00
-GCC	59	34.00	23.00	24.00	19.00
-GCC	60	29.00	28.00	17.00	26.00
-GCC	61	25.50	23.50	24.50	26.50
-GCC	62	31.50	22.50	16.50	29.50
-GCC	63	27.50	28.50	25.50	18.50
-GCC	64	33.50	21.50	25.50	19.50
-GCC	65	35.50	19.50	18.50	26.50
-GCC	66	34.00	25.00	15.00	26.00
-GCC	67	37.00	23.00	19.00	21.00
-GCC	68	36.50	29.50	13.50	20.50
-GCC	69	38.50	19.50	20.50	21.50
-GCC	70	38.50	16.50	18.50	26.50
-GCC	71	25.50	38.50	21.50	14.50
-GCC	72	29.00	29.00	25.00	17.00
-GCC	73	32.50	20.50	21.50	25.50
-GCC	74	28.50	32.50	12.50	26.50
-GCC	75	41.50	12.50	18.50	27.50
-GCC	76	24.50	29.50	23.50	22.50
-GCC	77	36.00	21.00	18.00	25.00
-GCC	78	27.00	34.00	22.00	17.00
-GCC	79	21.50	26.50	25.50	26.50
-GCC	80	34.00	19.00	28.00	19.00
-GCC	81	17.00	26.00	26.00	31.00
-GCC	82	31.00	30.00	23.00	16.00
-GCC	83	31.50	26.50	12.50	29.50
-GCC	84	19.00	41.00	21.00	19.00
-GCC	85	37.50	24.50	16.50	21.50
-GCC	86	15.00	48.00	15.00	22.00
-GCC	87	41.00	16.00	18.00	25.00
-GCC	88	23.50	27.50	27.50	21.50
-GCC	89	26.50	27.50	26.50	19.50
-GCC	90	18.50	23.50	24.50	33.50
-GCC	91	27.00	32.00	22.00	19.00
-GCC	92	23.50	17.50	27.50	31.50
-GCC	93	25.50	37.50	15.50	21.50
-GCC	94	27.00	17.00	24.00	32.00
-GCC	95	26.50	37.50	14.50	21.50
-GCC	96	29.50	25.50	16.50	28.50
-GCC	97	29.00	31.00	21.00	19.00
-GCC	98	18.00	33.00	22.00	27.00
-GCC	99	24.50	33.50	24.50	17.50
-GCC	100	24.50	16.50	24.50	34.50
-GCC	101	25.00	40.00	19.00	16.00
-GCC	102	17.50	17.50	32.50	32.50
-GCC	103	31.00	26.00	16.00	27.00
-GCC	104	26.50	29.50	20.50	23.50
-GCC	105	34.00	33.00	21.00	12.00
-GCC	106	23.00	31.00	26.00	20.00
-GCC	107	17.50	35.50	23.50	23.50
-GCC	108	24.50	30.50	23.50	21.50
-GCC	109	17.00	31.00	22.00	30.00
-GCC	110	16.00	35.00	24.00	25.00
-GCC	111	24.00	32.00	23.00	21.00
-GCC	112	37.00	28.00	16.00	19.00
-GCC	113	19.50	22.50	32.50	25.50
-GCC	114	17.00	31.00	35.00	17.00
-GCC	115	29.50	24.50	23.50	22.50
-GCC	116	22.00	30.00	34.00	14.00
-GCC	117	27.00	23.00	19.00	31.00
-GCC	118	25.50	14.50	34.50	25.50
-GCC	119	22.50	34.50	20.50	22.50
-GCC	120	17.50	24.50	26.50	31.50
-GCC	121	27.50	33.50	22.50	16.50
-GCC	122	17.00	23.00	25.00	35.00
-GCC	123	23.50	46.50	11.50	18.50
-GCC	124	9.00	32.00	34.00	25.00
-GCC	125	24.00	27.00	19.00	30.00
-GCC	126	26.00	17.00	28.00	29.00
-GCC	127	26.50	16.50	21.50	35.50
-GCC	128	18.00	34.00	31.00	17.00
-GCC	129	25.50	25.50	27.50	21.50
-GCC	130	25.00	20.00	22.00	33.00
-GCC	131	17.50	39.50	24.50	18.50
-GCC	132	21.00	28.00	23.00	28.00
-GCC	133	13.50	31.50	35.50	19.50
-GCC	134	24.50	19.50	30.50	25.50
-GCC	135	16.50	23.50	30.50	29.50
-GCC	136	28.00	32.00	15.00	25.00
-GCC	137	22.50	21.50	30.50	25.50
-GCC	138	14.50	34.50	24.50	26.50
-GCC	139	20.50	29.50	24.50	25.50
-GCC	140	17.00	23.00	30.00	30.00
-GCC	141	20.50	23.50	25.50	30.50
-GCC	142	18.00	29.00	38.00	15.00
-GCC	143	22.00	24.00	27.00	27.00
-GCC	144	21.50	30.50	26.50	21.50
-GCC	145	22.00	21.00	29.00	28.00
-GCC	146	25.00	16.00	39.00	20.00
-GCC	147	26.50	22.50	30.50	20.50
-GCC	148	12.50	28.50	36.50	22.50
-GCC	149	26.50	23.50	23.50	26.50
-GCC	150	14.00	29.00	24.00	33.00
-GCC	151	19.50	30.50	32.50	17.50
-GCC	152	18.50	17.50	29.50	34.50
-GCC	153	22.50	22.50	31.50	23.50
-GCC	154	22.00	21.00	29.00	28.00
-GCC	155	21.00	26.00	19.00	34.00
-GCC	156	14.50	23.50	35.50	26.50
-GCC	157	22.00	31.00	23.00	24.00
-GCC	158	22.50	29.50	24.50	23.50
-GCC	159	17.50	12.50	46.50	23.50
-GCC	160	24.50	26.50	26.50	22.50
-GCC	161	13.00	23.00	45.00	19.00
-GCC	162	31.50	16.50	22.50	29.50
-GCC	163	19.50	21.50	35.50	23.50
-GCC	164	29.00	18.00	21.00	32.00
-GCC	165	14.50	17.50	35.50	32.50
-GCC	166	17.50	37.50	28.50	16.50
-GCC	167	16.50	21.50	31.50	30.50
-GCC	168	14.00	25.00	30.00	31.00
-GCC	169	18.50	20.50	23.50	37.50
-GCC	170	19.00	23.00	28.00	30.00
-GCC	171	20.00	28.00	33.00	19.00
-GCC	172	19.00	20.00	32.00	29.00
-GCC	173	24.50	16.50	33.50	25.50
-GCC	174	17.50	23.50	33.50	25.50
-GCC	175	33.50	17.50	35.50	13.50
-GCC	176	16.50	32.50	28.50	22.50
-GCC	177	19.00	22.00	27.00	32.00
-GCC	178	16.00	26.00	30.00	28.00
-GCC	179	18.00	18.00	22.00	42.00
-GCC	180	21.00	22.00	34.00	23.00
-GCC	181	20.50	19.50	35.50	24.50
-GCC	182	32.50	18.50	22.50	26.50
-GCC	183	24.50	13.50	28.50	33.50
-GCC	184	15.00	29.00	30.00	26.00
-GCC	185	15.00	32.00	33.00	20.00
-GCC	186	22.50	23.50	34.50	19.50
-GCC	187	19.00	14.00	40.00	27.00
-GCC	188	27.50	21.50	27.50	23.50
-GCC	189	17.00	22.00	34.00	27.00
-GCC	190	23.00	30.00	23.00	24.00
-GCC	191	25.00	22.00	28.00	25.00
-GCC	192	34.50	24.50	13.50	27.50
-GCC	193	18.50	25.50	25.50	30.50
-GCC	194	18.50	33.50	24.50	23.50
-GCC	195	16.00	26.00	23.00	35.00
-GCC	196	21.50	25.50	24.50	28.50
-GCC	197	20.00	21.00	23.00	36.00
-GCC	198	17.00	21.00	37.00	25.00
-GCC	199	20.50	18.50	25.50	35.50
-GCC	200	21.00	29.00	21.00	29.00
-GCC	201	27.00	21.00	23.00	29.00
-GCC	202	21.50	24.50	19.50	34.50
-GCC	203	21.50	24.50	26.50	27.50
-GCC	204	27.00	29.00	24.00	20.00
-GCC	205	19.50	21.50	22.50	36.50
-GCC	206	26.50	24.50	21.50	27.50
-GCC	207	22.50	21.50	19.50	36.50
-GCC	208	14.00	35.00	29.00	22.00
-GCC	209	16.00	23.00	12.00	49.00
-GCC	210	18.50	19.50	40.50	21.50
-GCC	211	26.00	20.00	22.00	32.00
-GCC	212	21.00	31.00	18.00	30.00
-GCC	213	24.00	15.00	31.00	30.00
-GCC	214	17.50	24.50	25.50	32.50
-GCC	215	26.00	24.00	23.00	27.00
-GCC	216	21.50	17.50	25.50	35.50
-GCC	217	26.00	29.00	17.00	28.00
-GCC	218	20.00	27.00	21.00	32.00
-GCC	219	17.00	21.00	21.00	41.00
-GCC	220	25.50	23.50	23.50	27.50
-GCC	221	21.50	23.50	20.50	34.50
-GCC	222	21.50	21.50	18.50	38.50
-GCC	223	20.00	27.00	28.00	25.00
-GCC	224	22.50	22.50	24.50	30.50
-GCC	225	14.50	35.50	30.50	19.50
-GCC	226	20.00	23.00	26.00	31.00
-GCC	227	20.50	24.50	23.50	31.50
-GCC	228	33.00	19.00	26.00	22.00
-GCC	229	22.50	24.50	18.50	34.50
-GCC	230	21.00	32.00	16.00	31.00
-GCC	231	23.00	28.00	30.00	19.00
-GCC	232	23.50	21.50	12.50	42.50
-GCC	233	21.00	27.00	25.00	27.00
-GCC	234	16.50	27.50	22.50	33.50
-GCC	235	20.00	15.00	28.00	37.00
-GCC	236	28.00	23.00	21.00	28.00
-GCC	237	20.50	19.50	22.50	37.50
-GCC	238	21.50	29.50	24.50	24.50
-GCC	239	20.00	8.00	17.00	55.00
-GCC	240	28.00	24.00	16.00	32.00
-GCC	241	22.50	22.50	16.50	38.50
-GCC	242	29.00	25.00	13.00	33.00
-GCC	243	22.50	15.50	23.50	38.50
-GCC	244	20.50	23.50	16.50	39.50
-GCC	245	28.00	23.00	19.00	30.00
-GCC	246	21.00	29.00	25.00	25.00
-GCC	247	32.00	14.00	13.00	41.00
-GCC	248	18.00	18.00	25.00	39.00
-GCC	249	25.00	23.00	21.00	31.00
-GCC	250	27.50	22.50	17.50	32.50
-GCC	251	13.50	20.50	36.50	29.50
+# ACGT content per cycle. Use `grep ^GCC | cut -f 2-` to extract this part. The columns are: cycle; A,C,G,T base counts as a percentage of all A/C/G/T bases [%]; and N and O counts as a percentage of all A/C/G/T bases [%]
+GCC	1	21.94	30.10	34.18	13.78	2.04	0.00
+GCC	2	30.00	16.00	11.00	43.00	0.00	0.00
+GCC	3	33.00	25.00	9.00	33.00	0.00	0.00
+GCC	4	17.00	29.00	13.00	41.00	0.00	0.00
+GCC	5	37.00	22.00	12.00	29.00	0.00	0.00
+GCC	6	36.00	26.00	17.00	21.00	0.00	0.00
+GCC	7	29.50	13.50	31.50	25.50	0.00	0.00
+GCC	8	50.50	14.50	19.50	15.50	0.00	0.00
+GCC	9	17.00	33.00	18.00	32.00	0.00	0.00
+GCC	10	37.00	14.00	21.00	28.00	0.00	0.00
+GCC	11	20.50	14.50	29.50	35.50	0.00	0.00
+GCC	12	30.00	24.00	22.00	24.00	0.00	0.00
+GCC	13	27.00	15.00	21.00	37.00	0.00	0.00
+GCC	14	24.24	22.22	26.26	27.27	1.01	0.00
+GCC	15	25.50	20.50	24.50	29.50	0.00	0.00
+GCC	16	31.00	15.00	20.00	34.00	0.00	0.00
+GCC	17	28.00	25.00	14.00	33.00	0.00	0.00
+GCC	18	30.50	28.50	19.50	21.50	0.00	0.00
+GCC	19	29.00	26.00	21.00	24.00	0.00	0.00
+GCC	20	22.50	23.50	17.50	36.50	0.00	0.00
+GCC	21	35.50	17.50	19.50	27.50	0.00	0.00
+GCC	22	37.50	28.50	15.50	18.50	0.00	0.00
+GCC	23	31.31	19.19	13.13	36.36	1.01	0.00
+GCC	24	37.00	12.00	22.00	29.00	0.00	0.00
+GCC	25	35.50	22.50	17.50	24.50	0.00	0.00
+GCC	26	33.50	18.50	15.50	32.50	0.00	0.00
+GCC	27	34.50	14.50	25.50	25.50	0.00	0.00
+GCC	28	31.00	14.00	24.00	31.00	0.00	0.00
+GCC	29	30.00	27.00	24.00	19.00	0.00	0.00
+GCC	30	31.00	20.00	14.00	35.00	0.00	0.00
+GCC	31	33.50	29.50	13.50	23.50	0.00	0.00
+GCC	32	42.50	20.50	19.50	17.50	0.00	0.00
+GCC	33	25.50	23.50	14.50	36.50	0.00	0.00
+GCC	34	39.50	16.50	20.50	23.50	0.00	0.00
+GCC	35	32.50	23.50	21.50	22.50	0.00	0.00
+GCC	36	42.00	25.00	16.00	17.00	0.00	0.00
+GCC	37	38.00	17.00	19.00	26.00	0.00	0.00
+GCC	38	24.00	26.00	25.00	25.00	0.00	0.00
+GCC	39	22.50	41.50	18.50	17.50	0.00	0.00
+GCC	40	32.00	16.00	21.00	31.00	0.00	0.00
+GCC	41	33.00	28.00	19.00	20.00	0.00	0.00
+GCC	42	30.50	25.50	19.50	24.50	0.00	0.00
+GCC	43	35.00	29.00	15.00	21.00	0.00	0.00
+GCC	44	20.00	27.00	22.00	31.00	0.00	0.00
+GCC	45	40.50	21.50	21.50	16.50	0.00	0.00
+GCC	46	26.50	20.50	22.50	30.50	0.00	0.00
+GCC	47	38.50	29.50	16.50	15.50	0.00	0.00
+GCC	48	27.50	24.50	17.50	30.50	0.00	0.00
+GCC	49	28.50	32.50	10.50	28.50	0.00	0.00
+GCC	50	46.50	20.50	9.50	23.50	0.00	0.00
+GCC	51	34.50	28.50	13.50	23.50	0.00	0.00
+GCC	52	41.50	23.50	20.50	14.50	0.00	0.00
+GCC	53	20.00	28.00	26.00	26.00	0.00	0.00
+GCC	54	31.50	18.50	24.50	25.50	0.00	0.00
+GCC	55	30.50	22.50	16.50	30.50	0.00	0.00
+GCC	56	33.50	22.50	13.50	30.50	0.00	0.00
+GCC	57	23.00	24.00	23.00	30.00	0.00	0.00
+GCC	58	25.00	37.00	19.00	19.00	0.00	0.00
+GCC	59	34.00	23.00	24.00	19.00	0.00	0.00
+GCC	60	29.00	28.00	17.00	26.00	0.00	0.00
+GCC	61	25.50	23.50	24.50	26.50	0.00	0.00
+GCC	62	31.50	22.50	16.50	29.50	0.00	0.00
+GCC	63	27.50	28.50	25.50	18.50	0.00	0.00
+GCC	64	33.50	21.50	25.50	19.50	0.00	0.00
+GCC	65	35.50	19.50	18.50	26.50	0.00	0.00
+GCC	66	34.00	25.00	15.00	26.00	0.00	0.00
+GCC	67	37.00	23.00	19.00	21.00	0.00	0.00
+GCC	68	36.50	29.50	13.50	20.50	0.00	0.00
+GCC	69	38.50	19.50	20.50	21.50	0.00	0.00
+GCC	70	38.50	16.50	18.50	26.50	0.00	0.00
+GCC	71	25.50	38.50	21.50	14.50	0.00	0.00
+GCC	72	29.00	29.00	25.00	17.00	0.00	0.00
+GCC	73	32.50	20.50	21.50	25.50	0.00	0.00
+GCC	74	28.50	32.50	12.50	26.50	0.00	0.00
+GCC	75	41.50	12.50	18.50	27.50	0.00	0.00
+GCC	76	24.50	29.50	23.50	22.50	0.00	0.00
+GCC	77	36.00	21.00	18.00	25.00	0.00	0.00
+GCC	78	27.00	34.00	22.00	17.00	0.00	0.00
+GCC	79	21.50	26.50	25.50	26.50	0.00	0.00
+GCC	80	34.00	19.00	28.00	19.00	0.00	0.00
+GCC	81	17.00	26.00	26.00	31.00	0.00	0.00
+GCC	82	31.00	30.00	23.00	16.00	0.00	0.00
+GCC	83	31.50	26.50	12.50	29.50	0.00	0.00
+GCC	84	19.00	41.00	21.00	19.00	0.00	0.00
+GCC	85	37.50	24.50	16.50	21.50	0.00	0.00
+GCC	86	15.00	48.00	15.00	22.00	0.00	0.00
+GCC	87	41.00	16.00	18.00	25.00	0.00	0.00
+GCC	88	23.50	27.50	27.50	21.50	0.00	0.00
+GCC	89	26.50	27.50	26.50	19.50	0.00	0.00
+GCC	90	18.50	23.50	24.50	33.50	0.00	0.00
+GCC	91	27.00	32.00	22.00	19.00	0.00	0.00
+GCC	92	23.50	17.50	27.50	31.50	0.00	0.00
+GCC	93	25.50	37.50	15.50	21.50	0.00	0.00
+GCC	94	27.00	17.00	24.00	32.00	0.00	0.00
+GCC	95	26.77	37.88	14.65	20.71	1.01	0.00
+GCC	96	29.50	25.50	16.50	28.50	0.00	0.00
+GCC	97	29.00	31.00	21.00	19.00	0.00	0.00
+GCC	98	18.18	33.33	22.22	26.26	1.01	0.00
+GCC	99	24.50	33.50	24.50	17.50	0.00	0.00
+GCC	100	24.50	16.50	24.50	34.50	0.00	0.00
+GCC	101	25.00	40.00	19.00	16.00	0.00	0.00
+GCC	102	17.50	17.50	32.50	32.50	0.00	0.00
+GCC	103	31.00	26.00	16.00	27.00	0.00	0.00
+GCC	104	26.50	29.50	20.50	23.50	0.00	0.00
+GCC	105	34.00	33.00	21.00	12.00	0.00	0.00
+GCC	106	23.00	31.00	26.00	20.00	0.00	0.00
+GCC	107	17.68	35.86	23.74	22.73	1.01	0.00
+GCC	108	24.75	30.81	23.74	20.71	1.01	0.00
+GCC	109	17.00	31.00	22.00	30.00	0.00	0.00
+GCC	110	16.16	35.35	24.24	24.24	1.01	0.00
+GCC	111	24.24	32.32	23.23	20.20	1.01	0.00
+GCC	112	37.37	28.28	16.16	18.18	1.01	0.00
+GCC	113	19.70	22.73	32.83	24.75	1.01	0.00
+GCC	114	17.00	31.00	35.00	17.00	0.00	0.00
+GCC	115	29.50	24.50	23.50	22.50	0.00	0.00
+GCC	116	22.00	30.00	34.00	14.00	0.00	0.00
+GCC	117	27.00	23.00	19.00	31.00	0.00	0.00
+GCC	118	25.50	14.50	34.50	25.50	0.00	0.00
+GCC	119	22.50	34.50	20.50	22.50	0.00	0.00
+GCC	120	17.50	24.50	26.50	31.50	0.00	0.00
+GCC	121	27.78	33.84	22.73	15.66	1.01	0.00
+GCC	122	17.00	23.00	25.00	35.00	0.00	0.00
+GCC	123	23.50	46.50	11.50	18.50	0.00	0.00
+GCC	124	9.00	32.00	34.00	25.00	0.00	0.00
+GCC	125	24.00	27.00	19.00	30.00	0.00	0.00
+GCC	126	26.00	17.00	28.00	29.00	0.00	0.00
+GCC	127	26.50	16.50	21.50	35.50	0.00	0.00
+GCC	128	18.00	34.00	31.00	17.00	0.00	0.00
+GCC	129	25.50	25.50	27.50	21.50	0.00	0.00
+GCC	130	25.00	20.00	22.00	33.00	0.00	0.00
+GCC	131	17.50	39.50	24.50	18.50	0.00	0.00
+GCC	132	21.00	28.00	23.00	28.00	0.00	0.00
+GCC	133	13.50	31.50	35.50	19.50	0.00	0.00
+GCC	134	24.50	19.50	30.50	25.50	0.00	0.00
+GCC	135	16.50	23.50	30.50	29.50	0.00	0.00
+GCC	136	28.00	32.00	15.00	25.00	0.00	0.00
+GCC	137	22.50	21.50	30.50	25.50	0.00	0.00
+GCC	138	14.50	34.50	24.50	26.50	0.00	0.00
+GCC	139	20.50	29.50	24.50	25.50	0.00	0.00
+GCC	140	17.00	23.00	30.00	30.00	0.00	0.00
+GCC	141	20.50	23.50	25.50	30.50	0.00	0.00
+GCC	142	18.00	29.00	38.00	15.00	0.00	0.00
+GCC	143	22.00	24.00	27.00	27.00	0.00	0.00
+GCC	144	21.50	30.50	26.50	21.50	0.00	0.00
+GCC	145	22.00	21.00	29.00	28.00	0.00	0.00
+GCC	146	25.00	16.00	39.00	20.00	0.00	0.00
+GCC	147	26.50	22.50	30.50	20.50	0.00	0.00
+GCC	148	12.50	28.50	36.50	22.50	0.00	0.00
+GCC	149	26.50	23.50	23.50	26.50	0.00	0.00
+GCC	150	14.00	29.00	24.00	33.00	0.00	0.00
+GCC	151	19.50	30.50	32.50	17.50	0.00	0.00
+GCC	152	18.50	17.50	29.50	34.50	0.00	0.00
+GCC	153	22.50	22.50	31.50	23.50	0.00	0.00
+GCC	154	22.00	21.00	29.00	28.00	0.00	0.00
+GCC	155	21.00	26.00	19.00	34.00	0.00	0.00
+GCC	156	14.50	23.50	35.50	26.50	0.00	0.00
+GCC	157	22.00	31.00	23.00	24.00	0.00	0.00
+GCC	158	22.50	29.50	24.50	23.50	0.00	0.00
+GCC	159	17.50	12.50	46.50	23.50	0.00	0.00
+GCC	160	24.50	26.50	26.50	22.50	0.00	0.00
+GCC	161	13.00	23.00	45.00	19.00	0.00	0.00
+GCC	162	31.50	16.50	22.50	29.50	0.00	0.00
+GCC	163	19.50	21.50	35.50	23.50	0.00	0.00
+GCC	164	29.00	18.00	21.00	32.00	0.00	0.00
+GCC	165	14.50	17.50	35.50	32.50	0.00	0.00
+GCC	166	17.50	37.50	28.50	16.50	0.00	0.00
+GCC	167	16.50	21.50	31.50	30.50	0.00	0.00
+GCC	168	14.00	25.00	30.00	31.00	0.00	0.00
+GCC	169	18.50	20.50	23.50	37.50	0.00	0.00
+GCC	170	19.00	23.00	28.00	30.00	0.00	0.00
+GCC	171	20.00	28.00	33.00	19.00	0.00	0.00
+GCC	172	19.00	20.00	32.00	29.00	0.00	0.00
+GCC	173	24.50	16.50	33.50	25.50	0.00	0.00
+GCC	174	17.50	23.50	33.50	25.50	0.00	0.00
+GCC	175	33.50	17.50	35.50	13.50	0.00	0.00
+GCC	176	16.50	32.50	28.50	22.50	0.00	0.00
+GCC	177	19.00	22.00	27.00	32.00	0.00	0.00
+GCC	178	16.00	26.00	30.00	28.00	0.00	0.00
+GCC	179	18.00	18.00	22.00	42.00	0.00	0.00
+GCC	180	21.00	22.00	34.00	23.00	0.00	0.00
+GCC	181	20.50	19.50	35.50	24.50	0.00	0.00
+GCC	182	32.50	18.50	22.50	26.50	0.00	0.00
+GCC	183	24.50	13.50	28.50	33.50	0.00	0.00
+GCC	184	15.00	29.00	30.00	26.00	0.00	0.00
+GCC	185	15.00	32.00	33.00	20.00	0.00	0.00
+GCC	186	22.50	23.50	34.50	19.50	0.00	0.00
+GCC	187	19.00	14.00	40.00	27.00	0.00	0.00
+GCC	188	27.50	21.50	27.50	23.50	0.00	0.00
+GCC	189	17.00	22.00	34.00	27.00	0.00	0.00
+GCC	190	23.00	30.00	23.00	24.00	0.00	0.00
+GCC	191	25.00	22.00	28.00	25.00	0.00	0.00
+GCC	192	34.50	24.50	13.50	27.50	0.00	0.00
+GCC	193	18.50	25.50	25.50	30.50	0.00	0.00
+GCC	194	18.50	33.50	24.50	23.50	0.00	0.00
+GCC	195	16.00	26.00	23.00	35.00	0.00	0.00
+GCC	196	21.50	25.50	24.50	28.50	0.00	0.00
+GCC	197	20.00	21.00	23.00	36.00	0.00	0.00
+GCC	198	17.00	21.00	37.00	25.00	0.00	0.00
+GCC	199	20.50	18.50	25.50	35.50	0.00	0.00
+GCC	200	21.00	29.00	21.00	29.00	0.00	0.00
+GCC	201	27.00	21.00	23.00	29.00	0.00	0.00
+GCC	202	21.50	24.50	19.50	34.50	0.00	0.00
+GCC	203	21.50	24.50	26.50	27.50	0.00	0.00
+GCC	204	27.00	29.00	24.00	20.00	0.00	0.00
+GCC	205	19.50	21.50	22.50	36.50	0.00	0.00
+GCC	206	26.50	24.50	21.50	27.50	0.00	0.00
+GCC	207	22.50	21.50	19.50	36.50	0.00	0.00
+GCC	208	14.00	35.00	29.00	22.00	0.00	0.00
+GCC	209	16.00	23.00	12.00	49.00	0.00	0.00
+GCC	210	18.50	19.50	40.50	21.50	0.00	0.00
+GCC	211	26.00	20.00	22.00	32.00	0.00	0.00
+GCC	212	21.00	31.00	18.00	30.00	0.00	0.00
+GCC	213	24.00	15.00	31.00	30.00	0.00	0.00
+GCC	214	17.50	24.50	25.50	32.50	0.00	0.00
+GCC	215	26.00	24.00	23.00	27.00	0.00	0.00
+GCC	216	21.50	17.50	25.50	35.50	0.00	0.00
+GCC	217	26.00	29.00	17.00	28.00	0.00	0.00
+GCC	218	20.00	27.00	21.00	32.00	0.00	0.00
+GCC	219	17.00	21.00	21.00	41.00	0.00	0.00
+GCC	220	25.50	23.50	23.50	27.50	0.00	0.00
+GCC	221	21.50	23.50	20.50	34.50	0.00	0.00
+GCC	222	21.50	21.50	18.50	38.50	0.00	0.00
+GCC	223	20.00	27.00	28.00	25.00	0.00	0.00
+GCC	224	22.50	22.50	24.50	30.50	0.00	0.00
+GCC	225	14.50	35.50	30.50	19.50	0.00	0.00
+GCC	226	20.00	23.00	26.00	31.00	0.00	0.00
+GCC	227	20.50	24.50	23.50	31.50	0.00	0.00
+GCC	228	33.00	19.00	26.00	22.00	0.00	0.00
+GCC	229	22.50	24.50	18.50	34.50	0.00	0.00
+GCC	230	21.00	32.00	16.00	31.00	0.00	0.00
+GCC	231	23.00	28.00	30.00	19.00	0.00	0.00
+GCC	232	23.50	21.50	12.50	42.50	0.00	0.00
+GCC	233	21.00	27.00	25.00	27.00	0.00	0.00
+GCC	234	16.50	27.50	22.50	33.50	0.00	0.00
+GCC	235	20.00	15.00	28.00	37.00	0.00	0.00
+GCC	236	28.00	23.00	21.00	28.00	0.00	0.00
+GCC	237	20.50	19.50	22.50	37.50	0.00	0.00
+GCC	238	21.50	29.50	24.50	24.50	0.00	0.00
+GCC	239	20.00	8.00	17.00	55.00	0.00	0.00
+GCC	240	28.00	24.00	16.00	32.00	0.00	0.00
+GCC	241	22.50	22.50	16.50	38.50	0.00	0.00
+GCC	242	29.00	25.00	13.00	33.00	0.00	0.00
+GCC	243	22.50	15.50	23.50	38.50	0.00	0.00
+GCC	244	20.50	23.50	16.50	39.50	0.00	0.00
+GCC	245	28.00	23.00	19.00	30.00	0.00	0.00
+GCC	246	21.00	29.00	25.00	25.00	0.00	0.00
+GCC	247	32.00	14.00	13.00	41.00	0.00	0.00
+GCC	248	18.00	18.00	25.00	39.00	0.00	0.00
+GCC	249	25.00	23.00	21.00	31.00	0.00	0.00
+GCC	250	27.50	22.50	17.50	32.50	0.00	0.00
+GCC	251	13.50	20.50	36.50	29.50	0.00	0.00
 # Insert sizes. Use `grep ^IS | cut -f 2-` to extract this part. The columns are: insert size, pairs total, inward oriented pairs, outward oriented pairs, other pairs
 # Read lengths. Use `grep ^RL | cut -f 2-` to extract this part. The columns are: read length, count
 RL	251	200
diff --git a/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2.tab b/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2.tab
index 7867a751100..90cfc888533 100644
--- a/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2.tab
+++ b/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2.tab
@@ -1,5 +1,6 @@
-# This file was produced by samtools stats (1.2+htslib-1.2.1) and can be plotted using plot-bamstats
-# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /Users/anton/galaxy-git/database/files/000/dataset_9.dat /Users/anton/galaxy-git/database/files/000/dataset_10.dat
+# This file was produced by samtools stats (1.3.1+htslib-1.3.1) and can be plotted using plot-bamstats
+# This file contains statistics for all reads.
+# The command line was:  stats --coverage 1,1000,1 --GC-depth 20000.0 --insert-size 8000 --most-inserts 0.99 --trim-quality 0 --ref-seq /tmp/tmpO1aTrb/files/000/dataset_5.dat /tmp/tmpO1aTrb/files/000/dataset_4.dat
 # CHK, Checksum	[2]Read Names	[3]Sequences	[4]Qualities
 # CHK, CRC32 of reads which passed filtering followed by addition (32bit overflow)
 CHK	1bd20fd8	58ad2167	29883386
@@ -836,258 +837,258 @@ GCL	51.51	4
 GCL	52.01	1
 GCL	52.51	0
 GCL	53.02	1
-# ACGT content per cycle. Use `grep ^GCC | cut -f 2-` to extract this part. The columns are: cycle, and A,C,G,T counts [%]
-GCC	1	21.50	29.50	33.50	15.50
-GCC	2	30.00	16.00	11.00	43.00
-GCC	3	33.00	25.00	9.00	33.00
-GCC	4	17.00	29.00	13.00	41.00
-GCC	5	37.00	22.00	12.00	29.00
-GCC	6	36.00	26.00	17.00	21.00
-GCC	7	29.50	13.50	31.50	25.50
-GCC	8	50.50	14.50	19.50	15.50
-GCC	9	17.00	33.00	18.00	32.00
-GCC	10	37.00	14.00	21.00	28.00
-GCC	11	20.50	14.50	29.50	35.50
-GCC	12	30.00	24.00	22.00	24.00
-GCC	13	27.00	15.00	21.00	37.00
-GCC	14	24.00	22.00	26.00	28.00
-GCC	15	25.50	20.50	24.50	29.50
-GCC	16	31.00	15.00	20.00	34.00
-GCC	17	28.00	25.00	14.00	33.00
-GCC	18	30.50	28.50	19.50	21.50
-GCC	19	29.00	26.00	21.00	24.00
-GCC	20	22.50	23.50	17.50	36.50
-GCC	21	35.50	17.50	19.50	27.50
-GCC	22	37.50	28.50	15.50	18.50
-GCC	23	31.00	19.00	13.00	37.00
-GCC	24	37.00	12.00	22.00	29.00
-GCC	25	35.50	22.50	17.50	24.50
-GCC	26	33.50	18.50	15.50	32.50
-GCC	27	34.50	14.50	25.50	25.50
-GCC	28	31.00	14.00	24.00	31.00
-GCC	29	30.00	27.00	24.00	19.00
-GCC	30	31.00	20.00	14.00	35.00
-GCC	31	33.50	29.50	13.50	23.50
-GCC	32	42.50	20.50	19.50	17.50
-GCC	33	25.50	23.50	14.50	36.50
-GCC	34	39.50	16.50	20.50	23.50
-GCC	35	32.50	23.50	21.50	22.50
-GCC	36	42.00	25.00	16.00	17.00
-GCC	37	38.00	17.00	19.00	26.00
-GCC	38	24.00	26.00	25.00	25.00
-GCC	39	22.50	41.50	18.50	17.50
-GCC	40	32.00	16.00	21.00	31.00
-GCC	41	33.00	28.00	19.00	20.00
-GCC	42	30.50	25.50	19.50	24.50
-GCC	43	35.00	29.00	15.00	21.00
-GCC	44	20.00	27.00	22.00	31.00
-GCC	45	40.50	21.50	21.50	16.50
-GCC	46	26.50	20.50	22.50	30.50
-GCC	47	38.50	29.50	16.50	15.50
-GCC	48	27.50	24.50	17.50	30.50
-GCC	49	28.50	32.50	10.50	28.50
-GCC	50	46.50	20.50	9.50	23.50
-GCC	51	34.50	28.50	13.50	23.50
-GCC	52	41.50	23.50	20.50	14.50
-GCC	53	20.00	28.00	26.00	26.00
-GCC	54	31.50	18.50	24.50	25.50
-GCC	55	30.50	22.50	16.50	30.50
-GCC	56	33.50	22.50	13.50	30.50
-GCC	57	23.00	24.00	23.00	30.00
-GCC	58	25.00	37.00	19.00	19.00
-GCC	59	34.00	23.00	24.00	19.00
-GCC	60	29.00	28.00	17.00	26.00
-GCC	61	25.50	23.50	24.50	26.50
-GCC	62	31.50	22.50	16.50	29.50
-GCC	63	27.50	28.50	25.50	18.50
-GCC	64	33.50	21.50	25.50	19.50
-GCC	65	35.50	19.50	18.50	26.50
-GCC	66	34.00	25.00	15.00	26.00
-GCC	67	37.00	23.00	19.00	21.00
-GCC	68	36.50	29.50	13.50	20.50
-GCC	69	38.50	19.50	20.50	21.50
-GCC	70	38.50	16.50	18.50	26.50
-GCC	71	25.50	38.50	21.50	14.50
-GCC	72	29.00	29.00	25.00	17.00
-GCC	73	32.50	20.50	21.50	25.50
-GCC	74	28.50	32.50	12.50	26.50
-GCC	75	41.50	12.50	18.50	27.50
-GCC	76	24.50	29.50	23.50	22.50
-GCC	77	36.00	21.00	18.00	25.00
-GCC	78	27.00	34.00	22.00	17.00
-GCC	79	21.50	26.50	25.50	26.50
-GCC	80	34.00	19.00	28.00	19.00
-GCC	81	17.00	26.00	26.00	31.00
-GCC	82	31.00	30.00	23.00	16.00
-GCC	83	31.50	26.50	12.50	29.50
-GCC	84	19.00	41.00	21.00	19.00
-GCC	85	37.50	24.50	16.50	21.50
-GCC	86	15.00	48.00	15.00	22.00
-GCC	87	41.00	16.00	18.00	25.00
-GCC	88	23.50	27.50	27.50	21.50
-GCC	89	26.50	27.50	26.50	19.50
-GCC	90	18.50	23.50	24.50	33.50
-GCC	91	27.00	32.00	22.00	19.00
-GCC	92	23.50	17.50	27.50	31.50
-GCC	93	25.50	37.50	15.50	21.50
-GCC	94	27.00	17.00	24.00	32.00
-GCC	95	26.50	37.50	14.50	21.50
-GCC	96	29.50	25.50	16.50	28.50
-GCC	97	29.00	31.00	21.00	19.00
-GCC	98	18.00	33.00	22.00	27.00
-GCC	99	24.50	33.50	24.50	17.50
-GCC	100	24.50	16.50	24.50	34.50
-GCC	101	25.00	40.00	19.00	16.00
-GCC	102	17.50	17.50	32.50	32.50
-GCC	103	31.00	26.00	16.00	27.00
-GCC	104	26.50	29.50	20.50	23.50
-GCC	105	34.00	33.00	21.00	12.00
-GCC	106	23.00	31.00	26.00	20.00
-GCC	107	17.50	35.50	23.50	23.50
-GCC	108	24.50	30.50	23.50	21.50
-GCC	109	17.00	31.00	22.00	30.00
-GCC	110	16.00	35.00	24.00	25.00
-GCC	111	24.00	32.00	23.00	21.00
-GCC	112	37.00	28.00	16.00	19.00
-GCC	113	19.50	22.50	32.50	25.50
-GCC	114	17.00	31.00	35.00	17.00
-GCC	115	29.50	24.50	23.50	22.50
-GCC	116	22.00	30.00	34.00	14.00
-GCC	117	27.00	23.00	19.00	31.00
-GCC	118	25.50	14.50	34.50	25.50
-GCC	119	22.50	34.50	20.50	22.50
-GCC	120	17.50	24.50	26.50	31.50
-GCC	121	27.50	33.50	22.50	16.50
-GCC	122	17.00	23.00	25.00	35.00
-GCC	123	23.50	46.50	11.50	18.50
-GCC	124	9.00	32.00	34.00	25.00
-GCC	125	24.00	27.00	19.00	30.00
-GCC	126	26.00	17.00	28.00	29.00
-GCC	127	26.50	16.50	21.50	35.50
-GCC	128	18.00	34.00	31.00	17.00
-GCC	129	25.50	25.50	27.50	21.50
-GCC	130	25.00	20.00	22.00	33.00
-GCC	131	17.50	39.50	24.50	18.50
-GCC	132	21.00	28.00	23.00	28.00
-GCC	133	13.50	31.50	35.50	19.50
-GCC	134	24.50	19.50	30.50	25.50
-GCC	135	16.50	23.50	30.50	29.50
-GCC	136	28.00	32.00	15.00	25.00
-GCC	137	22.50	21.50	30.50	25.50
-GCC	138	14.50	34.50	24.50	26.50
-GCC	139	20.50	29.50	24.50	25.50
-GCC	140	17.00	23.00	30.00	30.00
-GCC	141	20.50	23.50	25.50	30.50
-GCC	142	18.00	29.00	38.00	15.00
-GCC	143	22.00	24.00	27.00	27.00
-GCC	144	21.50	30.50	26.50	21.50
-GCC	145	22.00	21.00	29.00	28.00
-GCC	146	25.00	16.00	39.00	20.00
-GCC	147	26.50	22.50	30.50	20.50
-GCC	148	12.50	28.50	36.50	22.50
-GCC	149	26.50	23.50	23.50	26.50
-GCC	150	14.00	29.00	24.00	33.00
-GCC	151	19.50	30.50	32.50	17.50
-GCC	152	18.50	17.50	29.50	34.50
-GCC	153	22.50	22.50	31.50	23.50
-GCC	154	22.00	21.00	29.00	28.00
-GCC	155	21.00	26.00	19.00	34.00
-GCC	156	14.50	23.50	35.50	26.50
-GCC	157	22.00	31.00	23.00	24.00
-GCC	158	22.50	29.50	24.50	23.50
-GCC	159	17.50	12.50	46.50	23.50
-GCC	160	24.50	26.50	26.50	22.50
-GCC	161	13.00	23.00	45.00	19.00
-GCC	162	31.50	16.50	22.50	29.50
-GCC	163	19.50	21.50	35.50	23.50
-GCC	164	29.00	18.00	21.00	32.00
-GCC	165	14.50	17.50	35.50	32.50
-GCC	166	17.50	37.50	28.50	16.50
-GCC	167	16.50	21.50	31.50	30.50
-GCC	168	14.00	25.00	30.00	31.00
-GCC	169	18.50	20.50	23.50	37.50
-GCC	170	19.00	23.00	28.00	30.00
-GCC	171	20.00	28.00	33.00	19.00
-GCC	172	19.00	20.00	32.00	29.00
-GCC	173	24.50	16.50	33.50	25.50
-GCC	174	17.50	23.50	33.50	25.50
-GCC	175	33.50	17.50	35.50	13.50
-GCC	176	16.50	32.50	28.50	22.50
-GCC	177	19.00	22.00	27.00	32.00
-GCC	178	16.00	26.00	30.00	28.00
-GCC	179	18.00	18.00	22.00	42.00
-GCC	180	21.00	22.00	34.00	23.00
-GCC	181	20.50	19.50	35.50	24.50
-GCC	182	32.50	18.50	22.50	26.50
-GCC	183	24.50	13.50	28.50	33.50
-GCC	184	15.00	29.00	30.00	26.00
-GCC	185	15.00	32.00	33.00	20.00
-GCC	186	22.50	23.50	34.50	19.50
-GCC	187	19.00	14.00	40.00	27.00
-GCC	188	27.50	21.50	27.50	23.50
-GCC	189	17.00	22.00	34.00	27.00
-GCC	190	23.00	30.00	23.00	24.00
-GCC	191	25.00	22.00	28.00	25.00
-GCC	192	34.50	24.50	13.50	27.50
-GCC	193	18.50	25.50	25.50	30.50
-GCC	194	18.50	33.50	24.50	23.50
-GCC	195	16.00	26.00	23.00	35.00
-GCC	196	21.50	25.50	24.50	28.50
-GCC	197	20.00	21.00	23.00	36.00
-GCC	198	17.00	21.00	37.00	25.00
-GCC	199	20.50	18.50	25.50	35.50
-GCC	200	21.00	29.00	21.00	29.00
-GCC	201	27.00	21.00	23.00	29.00
-GCC	202	21.50	24.50	19.50	34.50
-GCC	203	21.50	24.50	26.50	27.50
-GCC	204	27.00	29.00	24.00	20.00
-GCC	205	19.50	21.50	22.50	36.50
-GCC	206	26.50	24.50	21.50	27.50
-GCC	207	22.50	21.50	19.50	36.50
-GCC	208	14.00	35.00	29.00	22.00
-GCC	209	16.00	23.00	12.00	49.00
-GCC	210	18.50	19.50	40.50	21.50
-GCC	211	26.00	20.00	22.00	32.00
-GCC	212	21.00	31.00	18.00	30.00
-GCC	213	24.00	15.00	31.00	30.00
-GCC	214	17.50	24.50	25.50	32.50
-GCC	215	26.00	24.00	23.00	27.00
-GCC	216	21.50	17.50	25.50	35.50
-GCC	217	26.00	29.00	17.00	28.00
-GCC	218	20.00	27.00	21.00	32.00
-GCC	219	17.00	21.00	21.00	41.00
-GCC	220	25.50	23.50	23.50	27.50
-GCC	221	21.50	23.50	20.50	34.50
-GCC	222	21.50	21.50	18.50	38.50
-GCC	223	20.00	27.00	28.00	25.00
-GCC	224	22.50	22.50	24.50	30.50
-GCC	225	14.50	35.50	30.50	19.50
-GCC	226	20.00	23.00	26.00	31.00
-GCC	227	20.50	24.50	23.50	31.50
-GCC	228	33.00	19.00	26.00	22.00
-GCC	229	22.50	24.50	18.50	34.50
-GCC	230	21.00	32.00	16.00	31.00
-GCC	231	23.00	28.00	30.00	19.00
-GCC	232	23.50	21.50	12.50	42.50
-GCC	233	21.00	27.00	25.00	27.00
-GCC	234	16.50	27.50	22.50	33.50
-GCC	235	20.00	15.00	28.00	37.00
-GCC	236	28.00	23.00	21.00	28.00
-GCC	237	20.50	19.50	22.50	37.50
-GCC	238	21.50	29.50	24.50	24.50
-GCC	239	20.00	8.00	17.00	55.00
-GCC	240	28.00	24.00	16.00	32.00
-GCC	241	22.50	22.50	16.50	38.50
-GCC	242	29.00	25.00	13.00	33.00
-GCC	243	22.50	15.50	23.50	38.50
-GCC	244	20.50	23.50	16.50	39.50
-GCC	245	28.00	23.00	19.00	30.00
-GCC	246	21.00	29.00	25.00	25.00
-GCC	247	32.00	14.00	13.00	41.00
-GCC	248	18.00	18.00	25.00	39.00
-GCC	249	25.00	23.00	21.00	31.00
-GCC	250	27.50	22.50	17.50	32.50
-GCC	251	13.50	20.50	36.50	29.50
+# ACGT content per cycle. Use `grep ^GCC | cut -f 2-` to extract this part. The columns are: cycle; A,C,G,T base counts as a percentage of all A/C/G/T bases [%]; and N and O counts as a percentage of all A/C/G/T bases [%]
+GCC	1	21.94	30.10	34.18	13.78	2.04	0.00
+GCC	2	30.00	16.00	11.00	43.00	0.00	0.00
+GCC	3	33.00	25.00	9.00	33.00	0.00	0.00
+GCC	4	17.00	29.00	13.00	41.00	0.00	0.00
+GCC	5	37.00	22.00	12.00	29.00	0.00	0.00
+GCC	6	36.00	26.00	17.00	21.00	0.00	0.00
+GCC	7	29.50	13.50	31.50	25.50	0.00	0.00
+GCC	8	50.50	14.50	19.50	15.50	0.00	0.00
+GCC	9	17.00	33.00	18.00	32.00	0.00	0.00
+GCC	10	37.00	14.00	21.00	28.00	0.00	0.00
+GCC	11	20.50	14.50	29.50	35.50	0.00	0.00
+GCC	12	30.00	24.00	22.00	24.00	0.00	0.00
+GCC	13	27.00	15.00	21.00	37.00	0.00	0.00
+GCC	14	24.24	22.22	26.26	27.27	1.01	0.00
+GCC	15	25.50	20.50	24.50	29.50	0.00	0.00
+GCC	16	31.00	15.00	20.00	34.00	0.00	0.00
+GCC	17	28.00	25.00	14.00	33.00	0.00	0.00
+GCC	18	30.50	28.50	19.50	21.50	0.00	0.00
+GCC	19	29.00	26.00	21.00	24.00	0.00	0.00
+GCC	20	22.50	23.50	17.50	36.50	0.00	0.00
+GCC	21	35.50	17.50	19.50	27.50	0.00	0.00
+GCC	22	37.50	28.50	15.50	18.50	0.00	0.00
+GCC	23	31.31	19.19	13.13	36.36	1.01	0.00
+GCC	24	37.00	12.00	22.00	29.00	0.00	0.00
+GCC	25	35.50	22.50	17.50	24.50	0.00	0.00
+GCC	26	33.50	18.50	15.50	32.50	0.00	0.00
+GCC	27	34.50	14.50	25.50	25.50	0.00	0.00
+GCC	28	31.00	14.00	24.00	31.00	0.00	0.00
+GCC	29	30.00	27.00	24.00	19.00	0.00	0.00
+GCC	30	31.00	20.00	14.00	35.00	0.00	0.00
+GCC	31	33.50	29.50	13.50	23.50	0.00	0.00
+GCC	32	42.50	20.50	19.50	17.50	0.00	0.00
+GCC	33	25.50	23.50	14.50	36.50	0.00	0.00
+GCC	34	39.50	16.50	20.50	23.50	0.00	0.00
+GCC	35	32.50	23.50	21.50	22.50	0.00	0.00
+GCC	36	42.00	25.00	16.00	17.00	0.00	0.00
+GCC	37	38.00	17.00	19.00	26.00	0.00	0.00
+GCC	38	24.00	26.00	25.00	25.00	0.00	0.00
+GCC	39	22.50	41.50	18.50	17.50	0.00	0.00
+GCC	40	32.00	16.00	21.00	31.00	0.00	0.00
+GCC	41	33.00	28.00	19.00	20.00	0.00	0.00
+GCC	42	30.50	25.50	19.50	24.50	0.00	0.00
+GCC	43	35.00	29.00	15.00	21.00	0.00	0.00
+GCC	44	20.00	27.00	22.00	31.00	0.00	0.00
+GCC	45	40.50	21.50	21.50	16.50	0.00	0.00
+GCC	46	26.50	20.50	22.50	30.50	0.00	0.00
+GCC	47	38.50	29.50	16.50	15.50	0.00	0.00
+GCC	48	27.50	24.50	17.50	30.50	0.00	0.00
+GCC	49	28.50	32.50	10.50	28.50	0.00	0.00
+GCC	50	46.50	20.50	9.50	23.50	0.00	0.00
+GCC	51	34.50	28.50	13.50	23.50	0.00	0.00
+GCC	52	41.50	23.50	20.50	14.50	0.00	0.00
+GCC	53	20.00	28.00	26.00	26.00	0.00	0.00
+GCC	54	31.50	18.50	24.50	25.50	0.00	0.00
+GCC	55	30.50	22.50	16.50	30.50	0.00	0.00
+GCC	56	33.50	22.50	13.50	30.50	0.00	0.00
+GCC	57	23.00	24.00	23.00	30.00	0.00	0.00
+GCC	58	25.00	37.00	19.00	19.00	0.00	0.00
+GCC	59	34.00	23.00	24.00	19.00	0.00	0.00
+GCC	60	29.00	28.00	17.00	26.00	0.00	0.00
+GCC	61	25.50	23.50	24.50	26.50	0.00	0.00
+GCC	62	31.50	22.50	16.50	29.50	0.00	0.00
+GCC	63	27.50	28.50	25.50	18.50	0.00	0.00
+GCC	64	33.50	21.50	25.50	19.50	0.00	0.00
+GCC	65	35.50	19.50	18.50	26.50	0.00	0.00
+GCC	66	34.00	25.00	15.00	26.00	0.00	0.00
+GCC	67	37.00	23.00	19.00	21.00	0.00	0.00
+GCC	68	36.50	29.50	13.50	20.50	0.00	0.00
+GCC	69	38.50	19.50	20.50	21.50	0.00	0.00
+GCC	70	38.50	16.50	18.50	26.50	0.00	0.00
+GCC	71	25.50	38.50	21.50	14.50	0.00	0.00
+GCC	72	29.00	29.00	25.00	17.00	0.00	0.00
+GCC	73	32.50	20.50	21.50	25.50	0.00	0.00
+GCC	74	28.50	32.50	12.50	26.50	0.00	0.00
+GCC	75	41.50	12.50	18.50	27.50	0.00	0.00
+GCC	76	24.50	29.50	23.50	22.50	0.00	0.00
+GCC	77	36.00	21.00	18.00	25.00	0.00	0.00
+GCC	78	27.00	34.00	22.00	17.00	0.00	0.00
+GCC	79	21.50	26.50	25.50	26.50	0.00	0.00
+GCC	80	34.00	19.00	28.00	19.00	0.00	0.00
+GCC	81	17.00	26.00	26.00	31.00	0.00	0.00
+GCC	82	31.00	30.00	23.00	16.00	0.00	0.00
+GCC	83	31.50	26.50	12.50	29.50	0.00	0.00
+GCC	84	19.00	41.00	21.00	19.00	0.00	0.00
+GCC	85	37.50	24.50	16.50	21.50	0.00	0.00
+GCC	86	15.00	48.00	15.00	22.00	0.00	0.00
+GCC	87	41.00	16.00	18.00	25.00	0.00	0.00
+GCC	88	23.50	27.50	27.50	21.50	0.00	0.00
+GCC	89	26.50	27.50	26.50	19.50	0.00	0.00
+GCC	90	18.50	23.50	24.50	33.50	0.00	0.00
+GCC	91	27.00	32.00	22.00	19.00	0.00	0.00
+GCC	92	23.50	17.50	27.50	31.50	0.00	0.00
+GCC	93	25.50	37.50	15.50	21.50	0.00	0.00
+GCC	94	27.00	17.00	24.00	32.00	0.00	0.00
+GCC	95	26.77	37.88	14.65	20.71	1.01	0.00
+GCC	96	29.50	25.50	16.50	28.50	0.00	0.00
+GCC	97	29.00	31.00	21.00	19.00	0.00	0.00
+GCC	98	18.18	33.33	22.22	26.26	1.01	0.00
+GCC	99	24.50	33.50	24.50	17.50	0.00	0.00
+GCC	100	24.50	16.50	24.50	34.50	0.00	0.00
+GCC	101	25.00	40.00	19.00	16.00	0.00	0.00
+GCC	102	17.50	17.50	32.50	32.50	0.00	0.00
+GCC	103	31.00	26.00	16.00	27.00	0.00	0.00
+GCC	104	26.50	29.50	20.50	23.50	0.00	0.00
+GCC	105	34.00	33.00	21.00	12.00	0.00	0.00
+GCC	106	23.00	31.00	26.00	20.00	0.00	0.00
+GCC	107	17.68	35.86	23.74	22.73	1.01	0.00
+GCC	108	24.75	30.81	23.74	20.71	1.01	0.00
+GCC	109	17.00	31.00	22.00	30.00	0.00	0.00
+GCC	110	16.16	35.35	24.24	24.24	1.01	0.00
+GCC	111	24.24	32.32	23.23	20.20	1.01	0.00
+GCC	112	37.37	28.28	16.16	18.18	1.01	0.00
+GCC	113	19.70	22.73	32.83	24.75	1.01	0.00
+GCC	114	17.00	31.00	35.00	17.00	0.00	0.00
+GCC	115	29.50	24.50	23.50	22.50	0.00	0.00
+GCC	116	22.00	30.00	34.00	14.00	0.00	0.00
+GCC	117	27.00	23.00	19.00	31.00	0.00	0.00
+GCC	118	25.50	14.50	34.50	25.50	0.00	0.00
+GCC	119	22.50	34.50	20.50	22.50	0.00	0.00
+GCC	120	17.50	24.50	26.50	31.50	0.00	0.00
+GCC	121	27.78	33.84	22.73	15.66	1.01	0.00
+GCC	122	17.00	23.00	25.00	35.00	0.00	0.00
+GCC	123	23.50	46.50	11.50	18.50	0.00	0.00
+GCC	124	9.00	32.00	34.00	25.00	0.00	0.00
+GCC	125	24.00	27.00	19.00	30.00	0.00	0.00
+GCC	126	26.00	17.00	28.00	29.00	0.00	0.00
+GCC	127	26.50	16.50	21.50	35.50	0.00	0.00
+GCC	128	18.00	34.00	31.00	17.00	0.00	0.00
+GCC	129	25.50	25.50	27.50	21.50	0.00	0.00
+GCC	130	25.00	20.00	22.00	33.00	0.00	0.00
+GCC	131	17.50	39.50	24.50	18.50	0.00	0.00
+GCC	132	21.00	28.00	23.00	28.00	0.00	0.00
+GCC	133	13.50	31.50	35.50	19.50	0.00	0.00
+GCC	134	24.50	19.50	30.50	25.50	0.00	0.00
+GCC	135	16.50	23.50	30.50	29.50	0.00	0.00
+GCC	136	28.00	32.00	15.00	25.00	0.00	0.00
+GCC	137	22.50	21.50	30.50	25.50	0.00	0.00
+GCC	138	14.50	34.50	24.50	26.50	0.00	0.00
+GCC	139	20.50	29.50	24.50	25.50	0.00	0.00
+GCC	140	17.00	23.00	30.00	30.00	0.00	0.00
+GCC	141	20.50	23.50	25.50	30.50	0.00	0.00
+GCC	142	18.00	29.00	38.00	15.00	0.00	0.00
+GCC	143	22.00	24.00	27.00	27.00	0.00	0.00
+GCC	144	21.50	30.50	26.50	21.50	0.00	0.00
+GCC	145	22.00	21.00	29.00	28.00	0.00	0.00
+GCC	146	25.00	16.00	39.00	20.00	0.00	0.00
+GCC	147	26.50	22.50	30.50	20.50	0.00	0.00
+GCC	148	12.50	28.50	36.50	22.50	0.00	0.00
+GCC	149	26.50	23.50	23.50	26.50	0.00	0.00
+GCC	150	14.00	29.00	24.00	33.00	0.00	0.00
+GCC	151	19.50	30.50	32.50	17.50	0.00	0.00
+GCC	152	18.50	17.50	29.50	34.50	0.00	0.00
+GCC	153	22.50	22.50	31.50	23.50	0.00	0.00
+GCC	154	22.00	21.00	29.00	28.00	0.00	0.00
+GCC	155	21.00	26.00	19.00	34.00	0.00	0.00
+GCC	156	14.50	23.50	35.50	26.50	0.00	0.00
+GCC	157	22.00	31.00	23.00	24.00	0.00	0.00
+GCC	158	22.50	29.50	24.50	23.50	0.00	0.00
+GCC	159	17.50	12.50	46.50	23.50	0.00	0.00
+GCC	160	24.50	26.50	26.50	22.50	0.00	0.00
+GCC	161	13.00	23.00	45.00	19.00	0.00	0.00
+GCC	162	31.50	16.50	22.50	29.50	0.00	0.00
+GCC	163	19.50	21.50	35.50	23.50	0.00	0.00
+GCC	164	29.00	18.00	21.00	32.00	0.00	0.00
+GCC	165	14.50	17.50	35.50	32.50	0.00	0.00
+GCC	166	17.50	37.50	28.50	16.50	0.00	0.00
+GCC	167	16.50	21.50	31.50	30.50	0.00	0.00
+GCC	168	14.00	25.00	30.00	31.00	0.00	0.00
+GCC	169	18.50	20.50	23.50	37.50	0.00	0.00
+GCC	170	19.00	23.00	28.00	30.00	0.00	0.00
+GCC	171	20.00	28.00	33.00	19.00	0.00	0.00
+GCC	172	19.00	20.00	32.00	29.00	0.00	0.00
+GCC	173	24.50	16.50	33.50	25.50	0.00	0.00
+GCC	174	17.50	23.50	33.50	25.50	0.00	0.00
+GCC	175	33.50	17.50	35.50	13.50	0.00	0.00
+GCC	176	16.50	32.50	28.50	22.50	0.00	0.00
+GCC	177	19.00	22.00	27.00	32.00	0.00	0.00
+GCC	178	16.00	26.00	30.00	28.00	0.00	0.00
+GCC	179	18.00	18.00	22.00	42.00	0.00	0.00
+GCC	180	21.00	22.00	34.00	23.00	0.00	0.00
+GCC	181	20.50	19.50	35.50	24.50	0.00	0.00
+GCC	182	32.50	18.50	22.50	26.50	0.00	0.00
+GCC	183	24.50	13.50	28.50	33.50	0.00	0.00
+GCC	184	15.00	29.00	30.00	26.00	0.00	0.00
+GCC	185	15.00	32.00	33.00	20.00	0.00	0.00
+GCC	186	22.50	23.50	34.50	19.50	0.00	0.00
+GCC	187	19.00	14.00	40.00	27.00	0.00	0.00
+GCC	188	27.50	21.50	27.50	23.50	0.00	0.00
+GCC	189	17.00	22.00	34.00	27.00	0.00	0.00
+GCC	190	23.00	30.00	23.00	24.00	0.00	0.00
+GCC	191	25.00	22.00	28.00	25.00	0.00	0.00
+GCC	192	34.50	24.50	13.50	27.50	0.00	0.00
+GCC	193	18.50	25.50	25.50	30.50	0.00	0.00
+GCC	194	18.50	33.50	24.50	23.50	0.00	0.00
+GCC	195	16.00	26.00	23.00	35.00	0.00	0.00
+GCC	196	21.50	25.50	24.50	28.50	0.00	0.00
+GCC	197	20.00	21.00	23.00	36.00	0.00	0.00
+GCC	198	17.00	21.00	37.00	25.00	0.00	0.00
+GCC	199	20.50	18.50	25.50	35.50	0.00	0.00
+GCC	200	21.00	29.00	21.00	29.00	0.00	0.00
+GCC	201	27.00	21.00	23.00	29.00	0.00	0.00
+GCC	202	21.50	24.50	19.50	34.50	0.00	0.00
+GCC	203	21.50	24.50	26.50	27.50	0.00	0.00
+GCC	204	27.00	29.00	24.00	20.00	0.00	0.00
+GCC	205	19.50	21.50	22.50	36.50	0.00	0.00
+GCC	206	26.50	24.50	21.50	27.50	0.00	0.00
+GCC	207	22.50	21.50	19.50	36.50	0.00	0.00
+GCC	208	14.00	35.00	29.00	22.00	0.00	0.00
+GCC	209	16.00	23.00	12.00	49.00	0.00	0.00
+GCC	210	18.50	19.50	40.50	21.50	0.00	0.00
+GCC	211	26.00	20.00	22.00	32.00	0.00	0.00
+GCC	212	21.00	31.00	18.00	30.00	0.00	0.00
+GCC	213	24.00	15.00	31.00	30.00	0.00	0.00
+GCC	214	17.50	24.50	25.50	32.50	0.00	0.00
+GCC	215	26.00	24.00	23.00	27.00	0.00	0.00
+GCC	216	21.50	17.50	25.50	35.50	0.00	0.00
+GCC	217	26.00	29.00	17.00	28.00	0.00	0.00
+GCC	218	20.00	27.00	21.00	32.00	0.00	0.00
+GCC	219	17.00	21.00	21.00	41.00	0.00	0.00
+GCC	220	25.50	23.50	23.50	27.50	0.00	0.00
+GCC	221	21.50	23.50	20.50	34.50	0.00	0.00
+GCC	222	21.50	21.50	18.50	38.50	0.00	0.00
+GCC	223	20.00	27.00	28.00	25.00	0.00	0.00
+GCC	224	22.50	22.50	24.50	30.50	0.00	0.00
+GCC	225	14.50	35.50	30.50	19.50	0.00	0.00
+GCC	226	20.00	23.00	26.00	31.00	0.00	0.00
+GCC	227	20.50	24.50	23.50	31.50	0.00	0.00
+GCC	228	33.00	19.00	26.00	22.00	0.00	0.00
+GCC	229	22.50	24.50	18.50	34.50	0.00	0.00
+GCC	230	21.00	32.00	16.00	31.00	0.00	0.00
+GCC	231	23.00	28.00	30.00	19.00	0.00	0.00
+GCC	232	23.50	21.50	12.50	42.50	0.00	0.00
+GCC	233	21.00	27.00	25.00	27.00	0.00	0.00
+GCC	234	16.50	27.50	22.50	33.50	0.00	0.00
+GCC	235	20.00	15.00	28.00	37.00	0.00	0.00
+GCC	236	28.00	23.00	21.00	28.00	0.00	0.00
+GCC	237	20.50	19.50	22.50	37.50	0.00	0.00
+GCC	238	21.50	29.50	24.50	24.50	0.00	0.00
+GCC	239	20.00	8.00	17.00	55.00	0.00	0.00
+GCC	240	28.00	24.00	16.00	32.00	0.00	0.00
+GCC	241	22.50	22.50	16.50	38.50	0.00	0.00
+GCC	242	29.00	25.00	13.00	33.00	0.00	0.00
+GCC	243	22.50	15.50	23.50	38.50	0.00	0.00
+GCC	244	20.50	23.50	16.50	39.50	0.00	0.00
+GCC	245	28.00	23.00	19.00	30.00	0.00	0.00
+GCC	246	21.00	29.00	25.00	25.00	0.00	0.00
+GCC	247	32.00	14.00	13.00	41.00	0.00	0.00
+GCC	248	18.00	18.00	25.00	39.00	0.00	0.00
+GCC	249	25.00	23.00	21.00	31.00	0.00	0.00
+GCC	250	27.50	22.50	17.50	32.50	0.00	0.00
+GCC	251	13.50	20.50	36.50	29.50	0.00	0.00
 # Insert sizes. Use `grep ^IS | cut -f 2-` to extract this part. The columns are: insert size, pairs total, inward oriented pairs, outward oriented pairs, other pairs
 # Read lengths. Use `grep ^RL | cut -f 2-` to extract this part. The columns are: read length, count
 RL	251	200

From 46b0568395bfd04054b6d8b7c3c87b6fd420766a Mon Sep 17 00:00:00 2001
From: yhoogstrate <y.hoogstrate@gmail.com>
Date: Fri, 28 Apr 2017 15:39:13 +0200
Subject: [PATCH 40/40] samtools stats: moving test-data from sub-directory to
 allow properly updating it

---
 .../samtools_stats/samtools_stats.xml         |   6 +-
 .../test-data/samtools_stats_out2/gcc.tab     | 252 ------------------
 .../test-data/samtools_stats_out2__gcc.tab    | 252 ++++++++++++++++++
 .../mpc.tab => samtools_stats_out2__mpc.tab}  |   0
 .../sn.tab => samtools_stats_out2__sn.tab}    |   0
 5 files changed, 255 insertions(+), 255 deletions(-)
 delete mode 100644 tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2/gcc.tab
 create mode 100644 tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2__gcc.tab
 rename tool_collections/samtools/samtools_stats/test-data/{samtools_stats_out2/mpc.tab => samtools_stats_out2__mpc.tab} (100%)
 rename tool_collections/samtools/samtools_stats/test-data/{samtools_stats_out2/sn.tab => samtools_stats_out2__sn.tab} (100%)

diff --git a/tool_collections/samtools/samtools_stats/samtools_stats.xml b/tool_collections/samtools/samtools_stats/samtools_stats.xml
index bfb3ecb2a17..c6e330ed404 100644
--- a/tool_collections/samtools/samtools_stats/samtools_stats.xml
+++ b/tool_collections/samtools/samtools_stats/samtools_stats.xml
@@ -270,9 +270,9 @@
             <param name="split_output_selector" value="yes" />
             <param name="generate_tables" value="sn,mpc,gcc" />
             <output name="output" file="samtools_stats_out2.tab" lines_diff="4">
-                <discovered_dataset designation="Summary numbers" ftype="tabular" file="samtools_stats_out2/sn.tab" />
-                <discovered_dataset designation="ACGT content per cycle" ftype="tabular" file="samtools_stats_out2/gcc.tab" />
-                <discovered_dataset designation="Mismatches per cycle" ftype="tabular" file="samtools_stats_out2/mpc.tab" />
+                <discovered_dataset designation="Summary numbers" ftype="tabular" file="samtools_stats_out2__sn.tab" />
+                <discovered_dataset designation="ACGT content per cycle" ftype="tabular" file="samtools_stats_out2__gcc.tab" />
+                <discovered_dataset designation="Mismatches per cycle" ftype="tabular" file="samtools_stats_out2__mpc.tab" />
             </output>
         </test>
     </tests>
diff --git a/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2/gcc.tab b/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2/gcc.tab
deleted file mode 100644
index e36f8f21ca3..00000000000
--- a/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2/gcc.tab
+++ /dev/null
@@ -1,252 +0,0 @@
-# ACGT content per cycle. The columns are: cycle, and A,C,G,T counts (percent)
-1	21.50	29.50	33.50	15.50
-2	30.00	16.00	11.00	43.00
-3	33.00	25.00	9.00	33.00
-4	17.00	29.00	13.00	41.00
-5	37.00	22.00	12.00	29.00
-6	36.00	26.00	17.00	21.00
-7	29.50	13.50	31.50	25.50
-8	50.50	14.50	19.50	15.50
-9	17.00	33.00	18.00	32.00
-10	37.00	14.00	21.00	28.00
-11	20.50	14.50	29.50	35.50
-12	30.00	24.00	22.00	24.00
-13	27.00	15.00	21.00	37.00
-14	24.00	22.00	26.00	28.00
-15	25.50	20.50	24.50	29.50
-16	31.00	15.00	20.00	34.00
-17	28.00	25.00	14.00	33.00
-18	30.50	28.50	19.50	21.50
-19	29.00	26.00	21.00	24.00
-20	22.50	23.50	17.50	36.50
-21	35.50	17.50	19.50	27.50
-22	37.50	28.50	15.50	18.50
-23	31.00	19.00	13.00	37.00
-24	37.00	12.00	22.00	29.00
-25	35.50	22.50	17.50	24.50
-26	33.50	18.50	15.50	32.50
-27	34.50	14.50	25.50	25.50
-28	31.00	14.00	24.00	31.00
-29	30.00	27.00	24.00	19.00
-30	31.00	20.00	14.00	35.00
-31	33.50	29.50	13.50	23.50
-32	42.50	20.50	19.50	17.50
-33	25.50	23.50	14.50	36.50
-34	39.50	16.50	20.50	23.50
-35	32.50	23.50	21.50	22.50
-36	42.00	25.00	16.00	17.00
-37	38.00	17.00	19.00	26.00
-38	24.00	26.00	25.00	25.00
-39	22.50	41.50	18.50	17.50
-40	32.00	16.00	21.00	31.00
-41	33.00	28.00	19.00	20.00
-42	30.50	25.50	19.50	24.50
-43	35.00	29.00	15.00	21.00
-44	20.00	27.00	22.00	31.00
-45	40.50	21.50	21.50	16.50
-46	26.50	20.50	22.50	30.50
-47	38.50	29.50	16.50	15.50
-48	27.50	24.50	17.50	30.50
-49	28.50	32.50	10.50	28.50
-50	46.50	20.50	9.50	23.50
-51	34.50	28.50	13.50	23.50
-52	41.50	23.50	20.50	14.50
-53	20.00	28.00	26.00	26.00
-54	31.50	18.50	24.50	25.50
-55	30.50	22.50	16.50	30.50
-56	33.50	22.50	13.50	30.50
-57	23.00	24.00	23.00	30.00
-58	25.00	37.00	19.00	19.00
-59	34.00	23.00	24.00	19.00
-60	29.00	28.00	17.00	26.00
-61	25.50	23.50	24.50	26.50
-62	31.50	22.50	16.50	29.50
-63	27.50	28.50	25.50	18.50
-64	33.50	21.50	25.50	19.50
-65	35.50	19.50	18.50	26.50
-66	34.00	25.00	15.00	26.00
-67	37.00	23.00	19.00	21.00
-68	36.50	29.50	13.50	20.50
-69	38.50	19.50	20.50	21.50
-70	38.50	16.50	18.50	26.50
-71	25.50	38.50	21.50	14.50
-72	29.00	29.00	25.00	17.00
-73	32.50	20.50	21.50	25.50
-74	28.50	32.50	12.50	26.50
-75	41.50	12.50	18.50	27.50
-76	24.50	29.50	23.50	22.50
-77	36.00	21.00	18.00	25.00
-78	27.00	34.00	22.00	17.00
-79	21.50	26.50	25.50	26.50
-80	34.00	19.00	28.00	19.00
-81	17.00	26.00	26.00	31.00
-82	31.00	30.00	23.00	16.00
-83	31.50	26.50	12.50	29.50
-84	19.00	41.00	21.00	19.00
-85	37.50	24.50	16.50	21.50
-86	15.00	48.00	15.00	22.00
-87	41.00	16.00	18.00	25.00
-88	23.50	27.50	27.50	21.50
-89	26.50	27.50	26.50	19.50
-90	18.50	23.50	24.50	33.50
-91	27.00	32.00	22.00	19.00
-92	23.50	17.50	27.50	31.50
-93	25.50	37.50	15.50	21.50
-94	27.00	17.00	24.00	32.00
-95	26.50	37.50	14.50	21.50
-96	29.50	25.50	16.50	28.50
-97	29.00	31.00	21.00	19.00
-98	18.00	33.00	22.00	27.00
-99	24.50	33.50	24.50	17.50
-100	24.50	16.50	24.50	34.50
-101	25.00	40.00	19.00	16.00
-102	17.50	17.50	32.50	32.50
-103	31.00	26.00	16.00	27.00
-104	26.50	29.50	20.50	23.50
-105	34.00	33.00	21.00	12.00
-106	23.00	31.00	26.00	20.00
-107	17.50	35.50	23.50	23.50
-108	24.50	30.50	23.50	21.50
-109	17.00	31.00	22.00	30.00
-110	16.00	35.00	24.00	25.00
-111	24.00	32.00	23.00	21.00
-112	37.00	28.00	16.00	19.00
-113	19.50	22.50	32.50	25.50
-114	17.00	31.00	35.00	17.00
-115	29.50	24.50	23.50	22.50
-116	22.00	30.00	34.00	14.00
-117	27.00	23.00	19.00	31.00
-118	25.50	14.50	34.50	25.50
-119	22.50	34.50	20.50	22.50
-120	17.50	24.50	26.50	31.50
-121	27.50	33.50	22.50	16.50
-122	17.00	23.00	25.00	35.00
-123	23.50	46.50	11.50	18.50
-124	9.00	32.00	34.00	25.00
-125	24.00	27.00	19.00	30.00
-126	26.00	17.00	28.00	29.00
-127	26.50	16.50	21.50	35.50
-128	18.00	34.00	31.00	17.00
-129	25.50	25.50	27.50	21.50
-130	25.00	20.00	22.00	33.00
-131	17.50	39.50	24.50	18.50
-132	21.00	28.00	23.00	28.00
-133	13.50	31.50	35.50	19.50
-134	24.50	19.50	30.50	25.50
-135	16.50	23.50	30.50	29.50
-136	28.00	32.00	15.00	25.00
-137	22.50	21.50	30.50	25.50
-138	14.50	34.50	24.50	26.50
-139	20.50	29.50	24.50	25.50
-140	17.00	23.00	30.00	30.00
-141	20.50	23.50	25.50	30.50
-142	18.00	29.00	38.00	15.00
-143	22.00	24.00	27.00	27.00
-144	21.50	30.50	26.50	21.50
-145	22.00	21.00	29.00	28.00
-146	25.00	16.00	39.00	20.00
-147	26.50	22.50	30.50	20.50
-148	12.50	28.50	36.50	22.50
-149	26.50	23.50	23.50	26.50
-150	14.00	29.00	24.00	33.00
-151	19.50	30.50	32.50	17.50
-152	18.50	17.50	29.50	34.50
-153	22.50	22.50	31.50	23.50
-154	22.00	21.00	29.00	28.00
-155	21.00	26.00	19.00	34.00
-156	14.50	23.50	35.50	26.50
-157	22.00	31.00	23.00	24.00
-158	22.50	29.50	24.50	23.50
-159	17.50	12.50	46.50	23.50
-160	24.50	26.50	26.50	22.50
-161	13.00	23.00	45.00	19.00
-162	31.50	16.50	22.50	29.50
-163	19.50	21.50	35.50	23.50
-164	29.00	18.00	21.00	32.00
-165	14.50	17.50	35.50	32.50
-166	17.50	37.50	28.50	16.50
-167	16.50	21.50	31.50	30.50
-168	14.00	25.00	30.00	31.00
-169	18.50	20.50	23.50	37.50
-170	19.00	23.00	28.00	30.00
-171	20.00	28.00	33.00	19.00
-172	19.00	20.00	32.00	29.00
-173	24.50	16.50	33.50	25.50
-174	17.50	23.50	33.50	25.50
-175	33.50	17.50	35.50	13.50
-176	16.50	32.50	28.50	22.50
-177	19.00	22.00	27.00	32.00
-178	16.00	26.00	30.00	28.00
-179	18.00	18.00	22.00	42.00
-180	21.00	22.00	34.00	23.00
-181	20.50	19.50	35.50	24.50
-182	32.50	18.50	22.50	26.50
-183	24.50	13.50	28.50	33.50
-184	15.00	29.00	30.00	26.00
-185	15.00	32.00	33.00	20.00
-186	22.50	23.50	34.50	19.50
-187	19.00	14.00	40.00	27.00
-188	27.50	21.50	27.50	23.50
-189	17.00	22.00	34.00	27.00
-190	23.00	30.00	23.00	24.00
-191	25.00	22.00	28.00	25.00
-192	34.50	24.50	13.50	27.50
-193	18.50	25.50	25.50	30.50
-194	18.50	33.50	24.50	23.50
-195	16.00	26.00	23.00	35.00
-196	21.50	25.50	24.50	28.50
-197	20.00	21.00	23.00	36.00
-198	17.00	21.00	37.00	25.00
-199	20.50	18.50	25.50	35.50
-200	21.00	29.00	21.00	29.00
-201	27.00	21.00	23.00	29.00
-202	21.50	24.50	19.50	34.50
-203	21.50	24.50	26.50	27.50
-204	27.00	29.00	24.00	20.00
-205	19.50	21.50	22.50	36.50
-206	26.50	24.50	21.50	27.50
-207	22.50	21.50	19.50	36.50
-208	14.00	35.00	29.00	22.00
-209	16.00	23.00	12.00	49.00
-210	18.50	19.50	40.50	21.50
-211	26.00	20.00	22.00	32.00
-212	21.00	31.00	18.00	30.00
-213	24.00	15.00	31.00	30.00
-214	17.50	24.50	25.50	32.50
-215	26.00	24.00	23.00	27.00
-216	21.50	17.50	25.50	35.50
-217	26.00	29.00	17.00	28.00
-218	20.00	27.00	21.00	32.00
-219	17.00	21.00	21.00	41.00
-220	25.50	23.50	23.50	27.50
-221	21.50	23.50	20.50	34.50
-222	21.50	21.50	18.50	38.50
-223	20.00	27.00	28.00	25.00
-224	22.50	22.50	24.50	30.50
-225	14.50	35.50	30.50	19.50
-226	20.00	23.00	26.00	31.00
-227	20.50	24.50	23.50	31.50
-228	33.00	19.00	26.00	22.00
-229	22.50	24.50	18.50	34.50
-230	21.00	32.00	16.00	31.00
-231	23.00	28.00	30.00	19.00
-232	23.50	21.50	12.50	42.50
-233	21.00	27.00	25.00	27.00
-234	16.50	27.50	22.50	33.50
-235	20.00	15.00	28.00	37.00
-236	28.00	23.00	21.00	28.00
-237	20.50	19.50	22.50	37.50
-238	21.50	29.50	24.50	24.50
-239	20.00	8.00	17.00	55.00
-240	28.00	24.00	16.00	32.00
-241	22.50	22.50	16.50	38.50
-242	29.00	25.00	13.00	33.00
-243	22.50	15.50	23.50	38.50
-244	20.50	23.50	16.50	39.50
-245	28.00	23.00	19.00	30.00
-246	21.00	29.00	25.00	25.00
-247	32.00	14.00	13.00	41.00
-248	18.00	18.00	25.00	39.00
-249	25.00	23.00	21.00	31.00
-250	27.50	22.50	17.50	32.50
-251	13.50	20.50	36.50	29.50
diff --git a/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2__gcc.tab b/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2__gcc.tab
new file mode 100644
index 00000000000..a1fd54eca93
--- /dev/null
+++ b/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2__gcc.tab
@@ -0,0 +1,252 @@
+# ACGT content per cycle. The columns are: cycle, and A,C,G,T counts (percent)
+1	21.94	30.10	34.18	13.78	2.04	0.00
+2	30.00	16.00	11.00	43.00	0.00	0.00
+3	33.00	25.00	9.00	33.00	0.00	0.00
+4	17.00	29.00	13.00	41.00	0.00	0.00
+5	37.00	22.00	12.00	29.00	0.00	0.00
+6	36.00	26.00	17.00	21.00	0.00	0.00
+7	29.50	13.50	31.50	25.50	0.00	0.00
+8	50.50	14.50	19.50	15.50	0.00	0.00
+9	17.00	33.00	18.00	32.00	0.00	0.00
+10	37.00	14.00	21.00	28.00	0.00	0.00
+11	20.50	14.50	29.50	35.50	0.00	0.00
+12	30.00	24.00	22.00	24.00	0.00	0.00
+13	27.00	15.00	21.00	37.00	0.00	0.00
+14	24.24	22.22	26.26	27.27	1.01	0.00
+15	25.50	20.50	24.50	29.50	0.00	0.00
+16	31.00	15.00	20.00	34.00	0.00	0.00
+17	28.00	25.00	14.00	33.00	0.00	0.00
+18	30.50	28.50	19.50	21.50	0.00	0.00
+19	29.00	26.00	21.00	24.00	0.00	0.00
+20	22.50	23.50	17.50	36.50	0.00	0.00
+21	35.50	17.50	19.50	27.50	0.00	0.00
+22	37.50	28.50	15.50	18.50	0.00	0.00
+23	31.31	19.19	13.13	36.36	1.01	0.00
+24	37.00	12.00	22.00	29.00	0.00	0.00
+25	35.50	22.50	17.50	24.50	0.00	0.00
+26	33.50	18.50	15.50	32.50	0.00	0.00
+27	34.50	14.50	25.50	25.50	0.00	0.00
+28	31.00	14.00	24.00	31.00	0.00	0.00
+29	30.00	27.00	24.00	19.00	0.00	0.00
+30	31.00	20.00	14.00	35.00	0.00	0.00
+31	33.50	29.50	13.50	23.50	0.00	0.00
+32	42.50	20.50	19.50	17.50	0.00	0.00
+33	25.50	23.50	14.50	36.50	0.00	0.00
+34	39.50	16.50	20.50	23.50	0.00	0.00
+35	32.50	23.50	21.50	22.50	0.00	0.00
+36	42.00	25.00	16.00	17.00	0.00	0.00
+37	38.00	17.00	19.00	26.00	0.00	0.00
+38	24.00	26.00	25.00	25.00	0.00	0.00
+39	22.50	41.50	18.50	17.50	0.00	0.00
+40	32.00	16.00	21.00	31.00	0.00	0.00
+41	33.00	28.00	19.00	20.00	0.00	0.00
+42	30.50	25.50	19.50	24.50	0.00	0.00
+43	35.00	29.00	15.00	21.00	0.00	0.00
+44	20.00	27.00	22.00	31.00	0.00	0.00
+45	40.50	21.50	21.50	16.50	0.00	0.00
+46	26.50	20.50	22.50	30.50	0.00	0.00
+47	38.50	29.50	16.50	15.50	0.00	0.00
+48	27.50	24.50	17.50	30.50	0.00	0.00
+49	28.50	32.50	10.50	28.50	0.00	0.00
+50	46.50	20.50	9.50	23.50	0.00	0.00
+51	34.50	28.50	13.50	23.50	0.00	0.00
+52	41.50	23.50	20.50	14.50	0.00	0.00
+53	20.00	28.00	26.00	26.00	0.00	0.00
+54	31.50	18.50	24.50	25.50	0.00	0.00
+55	30.50	22.50	16.50	30.50	0.00	0.00
+56	33.50	22.50	13.50	30.50	0.00	0.00
+57	23.00	24.00	23.00	30.00	0.00	0.00
+58	25.00	37.00	19.00	19.00	0.00	0.00
+59	34.00	23.00	24.00	19.00	0.00	0.00
+60	29.00	28.00	17.00	26.00	0.00	0.00
+61	25.50	23.50	24.50	26.50	0.00	0.00
+62	31.50	22.50	16.50	29.50	0.00	0.00
+63	27.50	28.50	25.50	18.50	0.00	0.00
+64	33.50	21.50	25.50	19.50	0.00	0.00
+65	35.50	19.50	18.50	26.50	0.00	0.00
+66	34.00	25.00	15.00	26.00	0.00	0.00
+67	37.00	23.00	19.00	21.00	0.00	0.00
+68	36.50	29.50	13.50	20.50	0.00	0.00
+69	38.50	19.50	20.50	21.50	0.00	0.00
+70	38.50	16.50	18.50	26.50	0.00	0.00
+71	25.50	38.50	21.50	14.50	0.00	0.00
+72	29.00	29.00	25.00	17.00	0.00	0.00
+73	32.50	20.50	21.50	25.50	0.00	0.00
+74	28.50	32.50	12.50	26.50	0.00	0.00
+75	41.50	12.50	18.50	27.50	0.00	0.00
+76	24.50	29.50	23.50	22.50	0.00	0.00
+77	36.00	21.00	18.00	25.00	0.00	0.00
+78	27.00	34.00	22.00	17.00	0.00	0.00
+79	21.50	26.50	25.50	26.50	0.00	0.00
+80	34.00	19.00	28.00	19.00	0.00	0.00
+81	17.00	26.00	26.00	31.00	0.00	0.00
+82	31.00	30.00	23.00	16.00	0.00	0.00
+83	31.50	26.50	12.50	29.50	0.00	0.00
+84	19.00	41.00	21.00	19.00	0.00	0.00
+85	37.50	24.50	16.50	21.50	0.00	0.00
+86	15.00	48.00	15.00	22.00	0.00	0.00
+87	41.00	16.00	18.00	25.00	0.00	0.00
+88	23.50	27.50	27.50	21.50	0.00	0.00
+89	26.50	27.50	26.50	19.50	0.00	0.00
+90	18.50	23.50	24.50	33.50	0.00	0.00
+91	27.00	32.00	22.00	19.00	0.00	0.00
+92	23.50	17.50	27.50	31.50	0.00	0.00
+93	25.50	37.50	15.50	21.50	0.00	0.00
+94	27.00	17.00	24.00	32.00	0.00	0.00
+95	26.77	37.88	14.65	20.71	1.01	0.00
+96	29.50	25.50	16.50	28.50	0.00	0.00
+97	29.00	31.00	21.00	19.00	0.00	0.00
+98	18.18	33.33	22.22	26.26	1.01	0.00
+99	24.50	33.50	24.50	17.50	0.00	0.00
+100	24.50	16.50	24.50	34.50	0.00	0.00
+101	25.00	40.00	19.00	16.00	0.00	0.00
+102	17.50	17.50	32.50	32.50	0.00	0.00
+103	31.00	26.00	16.00	27.00	0.00	0.00
+104	26.50	29.50	20.50	23.50	0.00	0.00
+105	34.00	33.00	21.00	12.00	0.00	0.00
+106	23.00	31.00	26.00	20.00	0.00	0.00
+107	17.68	35.86	23.74	22.73	1.01	0.00
+108	24.75	30.81	23.74	20.71	1.01	0.00
+109	17.00	31.00	22.00	30.00	0.00	0.00
+110	16.16	35.35	24.24	24.24	1.01	0.00
+111	24.24	32.32	23.23	20.20	1.01	0.00
+112	37.37	28.28	16.16	18.18	1.01	0.00
+113	19.70	22.73	32.83	24.75	1.01	0.00
+114	17.00	31.00	35.00	17.00	0.00	0.00
+115	29.50	24.50	23.50	22.50	0.00	0.00
+116	22.00	30.00	34.00	14.00	0.00	0.00
+117	27.00	23.00	19.00	31.00	0.00	0.00
+118	25.50	14.50	34.50	25.50	0.00	0.00
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diff --git a/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2/mpc.tab b/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2__mpc.tab
similarity index 100%
rename from tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2/mpc.tab
rename to tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2__mpc.tab
diff --git a/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2/sn.tab b/tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2__sn.tab
similarity index 100%
rename from tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2/sn.tab
rename to tool_collections/samtools/samtools_stats/test-data/samtools_stats_out2__sn.tab