diff --git a/src/adapter/cloudflare-workers/serve-static.test.ts b/src/adapter/cloudflare-workers/serve-static.test.ts
index 2ebff9c7e..79fce4fbf 100644
--- a/src/adapter/cloudflare-workers/serve-static.test.ts
+++ b/src/adapter/cloudflare-workers/serve-static.test.ts
@@ -11,6 +11,9 @@ const store: Record<string, string> = {
   'static-no-root/plain.abcdef.txt': 'That is plain.txt',
   'assets/static/options/foo.abcdef.txt': 'With options',
   'assets/.static/plain.abcdef.txt': 'In the dot',
+  'assets/static/video/morning-routine.abcdef.m3u8': 'Good morning',
+  'assets/static/video/morning-routine1.abcdef.ts': 'Good',
+  'assets/static/video/introduction.abcdef.mp4': 'Let me introduce myself',
 }
 const manifest = JSON.stringify({
   'assets/static/plain.txt': 'assets/static/plain.abcdef.txt',
@@ -123,6 +126,37 @@ describe('With `file` options', () => {
   })
 })
 
+describe('With `mime` options', () => {
+  const mime = {
+    m3u8: 'application/vnd.apple.mpegurl',
+    ts: 'video/mp2t',
+  }
+  const manifest = {
+    'assets/static/video/morning-routine.m3u8': 'assets/static/video/morning-routine.abcdef.m3u8',
+    'assets/static/video/morning-routine1.ts': 'assets/static/video/morning-routine1.abcdef.ts',
+    'assets/static/video/introduction.mp4': 'assets/static/video/introduction.abcdef.mp4',
+  }
+
+  const app = new Hono()
+  app.use('/static/*', serveStatic({ root: './assets', mime, manifest }))
+
+  it('Should return content-type of m3u8', async () => {
+    const res = await app.request('http://localhost/static/video/morning-routine.m3u8')
+    expect(res.status).toBe(200)
+    expect(res.headers.get('Content-Type')).toBe('application/vnd.apple.mpegurl')
+  })
+  it('Should return content-type of ts', async () => {
+    const res = await app.request('http://localhost/static/video/morning-routine1.ts')
+    expect(res.status).toBe(200)
+    expect(res.headers.get('Content-Type')).toBe('video/mp2t')
+  })
+  it('Should return content-type of default', async () => {
+    const res = await app.request('http://localhost/static/video/introduction.mp4')
+    expect(res.status).toBe(200)
+    expect(res.headers.get('Content-Type')).not.toBe('video/mp4')
+  })
+})
+
 describe('With middleware', () => {
   const app = new Hono()
   const md1 = async (c: Context, next: Next) => {
diff --git a/src/utils/mime.test.ts b/src/utils/mime.test.ts
index e360802c2..5d9cdc9bd 100644
--- a/src/utils/mime.test.ts
+++ b/src/utils/mime.test.ts
@@ -1,5 +1,10 @@
 import { getMimeType, getExtension } from './mime'
 
+const mime = {
+  m3u8: 'application/vnd.apple.mpegurl',
+  ts: 'video/mp2t',
+}
+
 describe('mime', () => {
   it('getMimeType', () => {
     expect(getMimeType('hello.txt')).toBe('text/plain; charset=utf-8')
@@ -11,6 +16,12 @@ describe('mime', () => {
     expect(getMimeType('indexjs.abcd')).toBeUndefined()
   })
 
+  it('getMimeType with custom mime', () => {
+    expect(getMimeType('morning-routine.m3u8', mime)).toBe('application/vnd.apple.mpegurl')
+    expect(getMimeType('morning-routine1.ts', mime)).toBe('video/mp2t')
+    expect(getMimeType('readme.txt', mime)).toBeUndefined()
+  })
+
   it('getExtension', () => {
     expect(getExtension('audio/aac')).toBe('aac')
     expect(getExtension('application/x-abiword')).toBe('abw')