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README.txt
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README.txt
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/************************************************************************/
/* */
/* ANALYSIS OF STORM DATA */
/* */
/* Copyright 2010-2011 by Joachim Schleicher and Ullrich Koethe */
/* */
/* Please direct questions, bug reports, and contributions to */
/* joachim.schleicher@iwr.uni-heidelberg.de */
/************************************************************************/
dSTORM microscopy can improve the resolution of conventional fluorescent
microscopy by one order of magnitude. The data analysis software has to
localize many thousand single molecule point-spread-functions to
reconstruct an image of the underlying structure.
Content of this archive:
storm/ sources for single-molecule-localization
and command-line utility to analyze .sif and .hdf5 input data
gui/ Qt-based graphical-user-interface (GUI) for selection of
input files and appropriate parameter(s)
python/ helper scripts to analyze and display the coordinate
lists generated by the utilities above
colorcomposer/
multi-channel measurements have to be aligned due to
chromatic aberrations. Tetraspeck-Beads are detected
automatically so that the images can be registrated easily.
See INSTALL for instructions on how to build and install
storm or storm-gui
More algorithmic details are available in the diploma thesis at
http://hci.iwr.uni-heidelberg.de/Staff/jschleic/