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Denovo repeat library

A comprehensive denovo repeat library was prepared for repeat characterization. Pipeline is adapted from Earwig genome project by Upendra Bhattarai. Scripts are linked below.

Workflow:

A. Repeat library preparation

  1. Repeatmoduler
  2. LTRharvest & LTRdigest
  3. TransposonPSI
  4. Sine database
  5. RepeatClassifier
  6. RepeatMasker

B. Genome annotation

Maker2 pipeline was used for genome annotation. mRNA-seq data is denovo assembled using Trinity. Other relavant publicly available datasets were downloaded and used as input.

  1. Processing mRNA-seq data
  2. GeneMark-ES
  3. Configuring and running Maker2 pipeline