diff --git a/lib/bio/twobit.rb b/lib/bio/twobit.rb
index defb61a..96f1945 100644
--- a/lib/bio/twobit.rb
+++ b/lib/bio/twobit.rb
@@ -24,22 +24,6 @@ def open(*args, **kwargs)
         end
         file
       end
-
-      # Here, const_missing is used instead of autoload.
-
-      def const_missing(name)
-        # prevents const_get from being called recursively many times.
-        @missing_const ||= []
-        super if @missing_const.include? name
-        @missing_const << name
-
-        path = File.join(__dir__, "twobit/references", "#{name.to_s.downcase}.rb")
-        if File.exist?(path)
-          require path
-          return const_get(name)
-        end
-        super
-      end
     end
 
     def initialize(fname, masked: false)
@@ -117,3 +101,5 @@ def download(output_path, url)
     end
   end
 end
+
+Dir[__dir__ + "/twobit/references/*.rb"].each { |file| require file }
\ No newline at end of file
diff --git a/lib/bio/twobit/references/ailmel1.rb b/lib/bio/twobit/references/ailmel1.rb
new file mode 100644
index 0000000..6644893
--- /dev/null
+++ b/lib/bio/twobit/references/ailmel1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class AilMel1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "ailMel1"
+        @metadata.name = "ailMel1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/ailMel1/bigZips/ailMel1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/allmis1.rb b/lib/bio/twobit/references/allmis1.rb
new file mode 100644
index 0000000..0097ed6
--- /dev/null
+++ b/lib/bio/twobit/references/allmis1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class AllMis1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "allMis1"
+        @metadata.name = "allMis1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/allMis1/bigZips/allMis1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/anocar2.rb b/lib/bio/twobit/references/anocar2.rb
new file mode 100644
index 0000000..c37533d
--- /dev/null
+++ b/lib/bio/twobit/references/anocar2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class AnoCar2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "anoCar2"
+        @metadata.name = "anoCar2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/anoCar2/bigZips/anoCar2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/anogam3.rb b/lib/bio/twobit/references/anogam3.rb
new file mode 100644
index 0000000..054bd3f
--- /dev/null
+++ b/lib/bio/twobit/references/anogam3.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class AnoGam3 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "anoGam3"
+        @metadata.name = "anoGam3"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/anoGam3/bigZips/anoGam3.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/apimel2.rb b/lib/bio/twobit/references/apimel2.rb
new file mode 100644
index 0000000..e456d89
--- /dev/null
+++ b/lib/bio/twobit/references/apimel2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class ApiMel2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "apiMel2"
+        @metadata.name = "apiMel2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/apiMel2/bigZips/apiMel2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/aplcal1.rb b/lib/bio/twobit/references/aplcal1.rb
new file mode 100644
index 0000000..6a41a72
--- /dev/null
+++ b/lib/bio/twobit/references/aplcal1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class AplCal1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "aplCal1"
+        @metadata.name = "aplCal1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/aplCal1/bigZips/aplCal1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/aptman1.rb b/lib/bio/twobit/references/aptman1.rb
new file mode 100644
index 0000000..7eee027
--- /dev/null
+++ b/lib/bio/twobit/references/aptman1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class AptMan1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "aptMan1"
+        @metadata.name = "aptMan1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/aptMan1/bigZips/aptMan1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/aquchr2.rb b/lib/bio/twobit/references/aquchr2.rb
new file mode 100644
index 0000000..93fab4c
--- /dev/null
+++ b/lib/bio/twobit/references/aquchr2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class AquChr2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "aquChr2"
+        @metadata.name = "aquChr2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/aquChr2/bigZips/aquChr2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/balacu1.rb b/lib/bio/twobit/references/balacu1.rb
new file mode 100644
index 0000000..5a1fc70
--- /dev/null
+++ b/lib/bio/twobit/references/balacu1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class BalAcu1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "balAcu1"
+        @metadata.name = "balAcu1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/balAcu1/bigZips/balAcu1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/bostau7.rb b/lib/bio/twobit/references/bostau7.rb
new file mode 100644
index 0000000..62a39be
--- /dev/null
+++ b/lib/bio/twobit/references/bostau7.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class BosTau7 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "bosTau7"
+        @metadata.name = "bosTau7"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/bosTau7/bigZips/bosTau7.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/braflo1.rb b/lib/bio/twobit/references/braflo1.rb
new file mode 100644
index 0000000..ffcb45e
--- /dev/null
+++ b/lib/bio/twobit/references/braflo1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class BraFlo1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "braFlo1"
+        @metadata.name = "braFlo1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/braFlo1/bigZips/braFlo1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/caejap1.rb b/lib/bio/twobit/references/caejap1.rb
new file mode 100644
index 0000000..ec521eb
--- /dev/null
+++ b/lib/bio/twobit/references/caejap1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class CaeJap1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "caeJap1"
+        @metadata.name = "caeJap1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/caeJap1/bigZips/caeJap1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/caepb2.rb b/lib/bio/twobit/references/caepb2.rb
new file mode 100644
index 0000000..b1431ed
--- /dev/null
+++ b/lib/bio/twobit/references/caepb2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class CaePb2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "caePb2"
+        @metadata.name = "caePb2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/caePb2/bigZips/caePb2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/caerem3.rb b/lib/bio/twobit/references/caerem3.rb
new file mode 100644
index 0000000..ee03ef3
--- /dev/null
+++ b/lib/bio/twobit/references/caerem3.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class CaeRem3 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "caeRem3"
+        @metadata.name = "caeRem3"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/caeRem3/bigZips/caeRem3.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/caljac4.rb b/lib/bio/twobit/references/caljac4.rb
new file mode 100644
index 0000000..de3d6bb
--- /dev/null
+++ b/lib/bio/twobit/references/caljac4.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class CalJac4 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "calJac4"
+        @metadata.name = "calJac4"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/calJac4/bigZips/calJac4.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/calmil1.rb b/lib/bio/twobit/references/calmil1.rb
new file mode 100644
index 0000000..e27e132
--- /dev/null
+++ b/lib/bio/twobit/references/calmil1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class CalMil1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "calMil1"
+        @metadata.name = "calMil1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/calMil1/bigZips/calMil1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/canfam4.rb b/lib/bio/twobit/references/canfam4.rb
new file mode 100644
index 0000000..4d6c481
--- /dev/null
+++ b/lib/bio/twobit/references/canfam4.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class CanFam4 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "canFam4"
+        @metadata.name = "canFam4"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/canFam4/bigZips/canFam4.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/canfam6.rb b/lib/bio/twobit/references/canfam6.rb
new file mode 100644
index 0000000..995d1f6
--- /dev/null
+++ b/lib/bio/twobit/references/canfam6.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class CanFam6 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "canFam6"
+        @metadata.name = "canFam6"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/canFam6/bigZips/canFam6.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/cb3.rb b/lib/bio/twobit/references/cb3.rb
new file mode 100644
index 0000000..3def7cc
--- /dev/null
+++ b/lib/bio/twobit/references/cb3.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class Cb3 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "cb3"
+        @metadata.name = "cb3"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/cb3/bigZips/cb3.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/ce11.rb b/lib/bio/twobit/references/ce11.rb
new file mode 100644
index 0000000..c8ddffb
--- /dev/null
+++ b/lib/bio/twobit/references/ce11.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class Ce11 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "ce11"
+        @metadata.name = "ce11"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/ce11/bigZips/ce11.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/cersim1.rb b/lib/bio/twobit/references/cersim1.rb
new file mode 100644
index 0000000..e509957
--- /dev/null
+++ b/lib/bio/twobit/references/cersim1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class CerSim1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "cerSim1"
+        @metadata.name = "cerSim1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/cerSim1/bigZips/cerSim1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/chlsab2.rb b/lib/bio/twobit/references/chlsab2.rb
new file mode 100644
index 0000000..7bfa46c
--- /dev/null
+++ b/lib/bio/twobit/references/chlsab2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class ChlSab2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "chlSab2"
+        @metadata.name = "chlSab2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/chlSab2/bigZips/chlSab2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/chohof1.rb b/lib/bio/twobit/references/chohof1.rb
new file mode 100644
index 0000000..8897670
--- /dev/null
+++ b/lib/bio/twobit/references/chohof1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class ChoHof1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "choHof1"
+        @metadata.name = "choHof1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/choHof1/bigZips/choHof1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/chrpic1.rb b/lib/bio/twobit/references/chrpic1.rb
new file mode 100644
index 0000000..69fc25a
--- /dev/null
+++ b/lib/bio/twobit/references/chrpic1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class ChrPic1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "chrPic1"
+        @metadata.name = "chrPic1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/chrPic1/bigZips/chrPic1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/ci2.rb b/lib/bio/twobit/references/ci2.rb
new file mode 100644
index 0000000..0d2f86e
--- /dev/null
+++ b/lib/bio/twobit/references/ci2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class Ci2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "ci2"
+        @metadata.name = "ci2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/ci2/bigZips/ci2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/crigrichov2.rb b/lib/bio/twobit/references/crigrichov2.rb
new file mode 100644
index 0000000..f48c59c
--- /dev/null
+++ b/lib/bio/twobit/references/crigrichov2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class CriGriChoV2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "criGriChoV2"
+        @metadata.name = "criGriChoV2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/criGriChoV2/bigZips/criGriChoV2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/dasnov3.rb b/lib/bio/twobit/references/dasnov3.rb
new file mode 100644
index 0000000..4b539aa
--- /dev/null
+++ b/lib/bio/twobit/references/dasnov3.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class DasNov3 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "dasNov3"
+        @metadata.name = "dasNov3"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/dasNov3/bigZips/dasNov3.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/dipord1.rb b/lib/bio/twobit/references/dipord1.rb
new file mode 100644
index 0000000..9ecf663
--- /dev/null
+++ b/lib/bio/twobit/references/dipord1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class DipOrd1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "dipOrd1"
+        @metadata.name = "dipOrd1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/dipOrd1/bigZips/dipOrd1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/dp3.rb b/lib/bio/twobit/references/dp3.rb
new file mode 100644
index 0000000..3220249
--- /dev/null
+++ b/lib/bio/twobit/references/dp3.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class Dp3 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "dp3"
+        @metadata.name = "dp3"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/dp3/bigZips/dp3.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/droana2.rb b/lib/bio/twobit/references/droana2.rb
new file mode 100644
index 0000000..864e162
--- /dev/null
+++ b/lib/bio/twobit/references/droana2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class DroAna2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "droAna2"
+        @metadata.name = "droAna2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/droAna2/bigZips/droAna2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/droere1.rb b/lib/bio/twobit/references/droere1.rb
new file mode 100644
index 0000000..7fc2094
--- /dev/null
+++ b/lib/bio/twobit/references/droere1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class DroEre1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "droEre1"
+        @metadata.name = "droEre1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/droEre1/bigZips/droEre1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/drogri1.rb b/lib/bio/twobit/references/drogri1.rb
new file mode 100644
index 0000000..8d94571
--- /dev/null
+++ b/lib/bio/twobit/references/drogri1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class DroGri1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "droGri1"
+        @metadata.name = "droGri1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/droGri1/bigZips/droGri1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/dromoj2.rb b/lib/bio/twobit/references/dromoj2.rb
new file mode 100644
index 0000000..197c09a
--- /dev/null
+++ b/lib/bio/twobit/references/dromoj2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class DroMoj2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "droMoj2"
+        @metadata.name = "droMoj2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/droMoj2/bigZips/droMoj2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/droper1.rb b/lib/bio/twobit/references/droper1.rb
new file mode 100644
index 0000000..608dffe
--- /dev/null
+++ b/lib/bio/twobit/references/droper1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class DroPer1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "droPer1"
+        @metadata.name = "droPer1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/droPer1/bigZips/droPer1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/drosec1.rb b/lib/bio/twobit/references/drosec1.rb
new file mode 100644
index 0000000..f089298
--- /dev/null
+++ b/lib/bio/twobit/references/drosec1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class DroSec1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "droSec1"
+        @metadata.name = "droSec1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/droSec1/bigZips/droSec1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/drosim1.rb b/lib/bio/twobit/references/drosim1.rb
new file mode 100644
index 0000000..fbe1553
--- /dev/null
+++ b/lib/bio/twobit/references/drosim1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class DroSim1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "droSim1"
+        @metadata.name = "droSim1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/droSim1/bigZips/droSim1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/drovir2.rb b/lib/bio/twobit/references/drovir2.rb
new file mode 100644
index 0000000..518c489
--- /dev/null
+++ b/lib/bio/twobit/references/drovir2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class DroVir2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "droVir2"
+        @metadata.name = "droVir2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/droVir2/bigZips/droVir2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/droyak2.rb b/lib/bio/twobit/references/droyak2.rb
new file mode 100644
index 0000000..ba4c749
--- /dev/null
+++ b/lib/bio/twobit/references/droyak2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class DroYak2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "droYak2"
+        @metadata.name = "droYak2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/droYak2/bigZips/droYak2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/ebovir3.rb b/lib/bio/twobit/references/ebovir3.rb
new file mode 100644
index 0000000..4dea53f
--- /dev/null
+++ b/lib/bio/twobit/references/ebovir3.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class EboVir3 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "eboVir3"
+        @metadata.name = "eboVir3"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/eboVir3/bigZips/eboVir3.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/echtel2.rb b/lib/bio/twobit/references/echtel2.rb
new file mode 100644
index 0000000..b4655b3
--- /dev/null
+++ b/lib/bio/twobit/references/echtel2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class EchTel2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "echTel2"
+        @metadata.name = "echTel2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/echTel2/bigZips/echTel2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/equcab3.rb b/lib/bio/twobit/references/equcab3.rb
new file mode 100644
index 0000000..89de88f
--- /dev/null
+++ b/lib/bio/twobit/references/equcab3.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class EquCab3 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "equCab3"
+        @metadata.name = "equCab3"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/equCab3/bigZips/equCab3.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/erieur2.rb b/lib/bio/twobit/references/erieur2.rb
new file mode 100644
index 0000000..6ea041d
--- /dev/null
+++ b/lib/bio/twobit/references/erieur2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class EriEur2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "eriEur2"
+        @metadata.name = "eriEur2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/eriEur2/bigZips/eriEur2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/felcat9.rb b/lib/bio/twobit/references/felcat9.rb
new file mode 100644
index 0000000..d2bd7fb
--- /dev/null
+++ b/lib/bio/twobit/references/felcat9.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class FelCat9 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "felCat9"
+        @metadata.name = "felCat9"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/felCat9/bigZips/felCat9.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/fr2.rb b/lib/bio/twobit/references/fr2.rb
new file mode 100644
index 0000000..fe300b5
--- /dev/null
+++ b/lib/bio/twobit/references/fr2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class Fr2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "fr2"
+        @metadata.name = "fr2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/fr2/bigZips/fr2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/gadmor1.rb b/lib/bio/twobit/references/gadmor1.rb
new file mode 100644
index 0000000..3a05375
--- /dev/null
+++ b/lib/bio/twobit/references/gadmor1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class GadMor1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "gadMor1"
+        @metadata.name = "gadMor1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/gadMor1/bigZips/gadMor1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/galgal6.rb b/lib/bio/twobit/references/galgal6.rb
new file mode 100644
index 0000000..d4f421e
--- /dev/null
+++ b/lib/bio/twobit/references/galgal6.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class GalGal6 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "galGal6"
+        @metadata.name = "galGal6"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/galGal6/bigZips/galGal6.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/geofor1.rb b/lib/bio/twobit/references/geofor1.rb
new file mode 100644
index 0000000..e576c66
--- /dev/null
+++ b/lib/bio/twobit/references/geofor1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class GeoFor1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "geoFor1"
+        @metadata.name = "geoFor1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/geoFor1/bigZips/geoFor1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/gorgor6.rb b/lib/bio/twobit/references/gorgor6.rb
new file mode 100644
index 0000000..b10dbc3
--- /dev/null
+++ b/lib/bio/twobit/references/gorgor6.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class GorGor6 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "gorGor6"
+        @metadata.name = "gorGor6"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/gorGor6/bigZips/gorGor6.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/hetgla2.rb b/lib/bio/twobit/references/hetgla2.rb
new file mode 100644
index 0000000..eeb167d
--- /dev/null
+++ b/lib/bio/twobit/references/hetgla2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class HetGla2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "hetGla2"
+        @metadata.name = "hetGla2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/hetGla2/bigZips/hetGla2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/latcha1.rb b/lib/bio/twobit/references/latcha1.rb
new file mode 100644
index 0000000..3f1ab93
--- /dev/null
+++ b/lib/bio/twobit/references/latcha1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class LatCha1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "latCha1"
+        @metadata.name = "latCha1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/latCha1/bigZips/latCha1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/loxafr3.rb b/lib/bio/twobit/references/loxafr3.rb
new file mode 100644
index 0000000..f71017c
--- /dev/null
+++ b/lib/bio/twobit/references/loxafr3.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class LoxAfr3 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "loxAfr3"
+        @metadata.name = "loxAfr3"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/loxAfr3/bigZips/loxAfr3.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/maceug2.rb b/lib/bio/twobit/references/maceug2.rb
new file mode 100644
index 0000000..f29f239
--- /dev/null
+++ b/lib/bio/twobit/references/maceug2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class MacEug2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "macEug2"
+        @metadata.name = "macEug2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/macEug2/bigZips/macEug2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/macfas5.rb b/lib/bio/twobit/references/macfas5.rb
new file mode 100644
index 0000000..b9fbf98
--- /dev/null
+++ b/lib/bio/twobit/references/macfas5.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class MacFas5 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "macFas5"
+        @metadata.name = "macFas5"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/macFas5/bigZips/macFas5.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/manpen1.rb b/lib/bio/twobit/references/manpen1.rb
new file mode 100644
index 0000000..9ee1ec7
--- /dev/null
+++ b/lib/bio/twobit/references/manpen1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class ManPen1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "manPen1"
+        @metadata.name = "manPen1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/manPen1/bigZips/manPen1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/melgal1.rb b/lib/bio/twobit/references/melgal1.rb
new file mode 100644
index 0000000..87d51b6
--- /dev/null
+++ b/lib/bio/twobit/references/melgal1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class MelGal1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "melGal1"
+        @metadata.name = "melGal1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/melGal1/bigZips/melGal1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/melund1.rb b/lib/bio/twobit/references/melund1.rb
new file mode 100644
index 0000000..2b0f3c4
--- /dev/null
+++ b/lib/bio/twobit/references/melund1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class MelUnd1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "melUnd1"
+        @metadata.name = "melUnd1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/melUnd1/bigZips/melUnd1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/micmur2.rb b/lib/bio/twobit/references/micmur2.rb
new file mode 100644
index 0000000..57f980e
--- /dev/null
+++ b/lib/bio/twobit/references/micmur2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class MicMur2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "micMur2"
+        @metadata.name = "micMur2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/micMur2/bigZips/micMur2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/mondom5.rb b/lib/bio/twobit/references/mondom5.rb
new file mode 100644
index 0000000..6135400
--- /dev/null
+++ b/lib/bio/twobit/references/mondom5.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class MonDom5 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "monDom5"
+        @metadata.name = "monDom5"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/monDom5/bigZips/monDom5.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/myoluc2.rb b/lib/bio/twobit/references/myoluc2.rb
new file mode 100644
index 0000000..c428e9c
--- /dev/null
+++ b/lib/bio/twobit/references/myoluc2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class MyoLuc2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "myoLuc2"
+        @metadata.name = "myoLuc2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/myoLuc2/bigZips/myoLuc2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/nanpar1.rb b/lib/bio/twobit/references/nanpar1.rb
new file mode 100644
index 0000000..91efb96
--- /dev/null
+++ b/lib/bio/twobit/references/nanpar1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class NanPar1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "nanPar1"
+        @metadata.name = "nanPar1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/nanPar1/bigZips/nanPar1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/naslar1.rb b/lib/bio/twobit/references/naslar1.rb
new file mode 100644
index 0000000..46f605a
--- /dev/null
+++ b/lib/bio/twobit/references/naslar1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class NasLar1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "nasLar1"
+        @metadata.name = "nasLar1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/nasLar1/bigZips/nasLar1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/neosch1.rb b/lib/bio/twobit/references/neosch1.rb
new file mode 100644
index 0000000..a25d91c
--- /dev/null
+++ b/lib/bio/twobit/references/neosch1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class NeoSch1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "neoSch1"
+        @metadata.name = "neoSch1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/neoSch1/bigZips/neoSch1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/nomleu3.rb b/lib/bio/twobit/references/nomleu3.rb
new file mode 100644
index 0000000..3c9cccd
--- /dev/null
+++ b/lib/bio/twobit/references/nomleu3.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class NomLeu3 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "nomLeu3"
+        @metadata.name = "nomLeu3"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/nomLeu3/bigZips/nomLeu3.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/ochpri3.rb b/lib/bio/twobit/references/ochpri3.rb
new file mode 100644
index 0000000..56bc822
--- /dev/null
+++ b/lib/bio/twobit/references/ochpri3.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class OchPri3 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "ochPri3"
+        @metadata.name = "ochPri3"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/ochPri3/bigZips/ochPri3.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/orenil2.rb b/lib/bio/twobit/references/orenil2.rb
new file mode 100644
index 0000000..cf8b8ae
--- /dev/null
+++ b/lib/bio/twobit/references/orenil2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class OreNil2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "oreNil2"
+        @metadata.name = "oreNil2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/oreNil2/bigZips/oreNil2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/ornana2.rb b/lib/bio/twobit/references/ornana2.rb
new file mode 100644
index 0000000..1cdecca
--- /dev/null
+++ b/lib/bio/twobit/references/ornana2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class OrnAna2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "ornAna2"
+        @metadata.name = "ornAna2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/ornAna2/bigZips/ornAna2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/orycun2.rb b/lib/bio/twobit/references/orycun2.rb
new file mode 100644
index 0000000..e473a1a
--- /dev/null
+++ b/lib/bio/twobit/references/orycun2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class OryCun2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "oryCun2"
+        @metadata.name = "oryCun2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/oryCun2/bigZips/oryCun2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/orylat2.rb b/lib/bio/twobit/references/orylat2.rb
new file mode 100644
index 0000000..84b99aa
--- /dev/null
+++ b/lib/bio/twobit/references/orylat2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class OryLat2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "oryLat2"
+        @metadata.name = "oryLat2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/oryLat2/bigZips/oryLat2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/otogar3.rb b/lib/bio/twobit/references/otogar3.rb
new file mode 100644
index 0000000..b9c7b16
--- /dev/null
+++ b/lib/bio/twobit/references/otogar3.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class OtoGar3 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "otoGar3"
+        @metadata.name = "otoGar3"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/otoGar3/bigZips/otoGar3.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/oviari4.rb b/lib/bio/twobit/references/oviari4.rb
new file mode 100644
index 0000000..4d0f4b1
--- /dev/null
+++ b/lib/bio/twobit/references/oviari4.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class OviAri4 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "oviAri4"
+        @metadata.name = "oviAri4"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/oviAri4/bigZips/oviAri4.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/panpan3.rb b/lib/bio/twobit/references/panpan3.rb
new file mode 100644
index 0000000..8b3477b
--- /dev/null
+++ b/lib/bio/twobit/references/panpan3.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class PanPan3 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "panPan3"
+        @metadata.name = "panPan3"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/panPan3/bigZips/panPan3.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/pantro6.rb b/lib/bio/twobit/references/pantro6.rb
new file mode 100644
index 0000000..50c7272
--- /dev/null
+++ b/lib/bio/twobit/references/pantro6.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class PanTro6 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "panTro6"
+        @metadata.name = "panTro6"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/panTro6/bigZips/panTro6.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/papanu4.rb b/lib/bio/twobit/references/papanu4.rb
new file mode 100644
index 0000000..0803782
--- /dev/null
+++ b/lib/bio/twobit/references/papanu4.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class PapAnu4 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "papAnu4"
+        @metadata.name = "papAnu4"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/papAnu4/bigZips/papAnu4.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/papham1.rb b/lib/bio/twobit/references/papham1.rb
new file mode 100644
index 0000000..327df5e
--- /dev/null
+++ b/lib/bio/twobit/references/papham1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class PapHam1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "papHam1"
+        @metadata.name = "papHam1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/papHam1/bigZips/papHam1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/petmar3.rb b/lib/bio/twobit/references/petmar3.rb
new file mode 100644
index 0000000..9d19acf
--- /dev/null
+++ b/lib/bio/twobit/references/petmar3.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class PetMar3 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "petMar3"
+        @metadata.name = "petMar3"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/petMar3/bigZips/petMar3.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/ponabe2.rb b/lib/bio/twobit/references/ponabe2.rb
new file mode 100644
index 0000000..2c115b4
--- /dev/null
+++ b/lib/bio/twobit/references/ponabe2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class PonAbe2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "ponAbe2"
+        @metadata.name = "ponAbe2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/ponAbe2/bigZips/ponAbe2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/ponabe3.rb b/lib/bio/twobit/references/ponabe3.rb
new file mode 100644
index 0000000..477909c
--- /dev/null
+++ b/lib/bio/twobit/references/ponabe3.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class PonAbe3 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "ponAbe3"
+        @metadata.name = "ponAbe3"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/ponAbe3/bigZips/ponAbe3.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/pripac1.rb b/lib/bio/twobit/references/pripac1.rb
new file mode 100644
index 0000000..3392a19
--- /dev/null
+++ b/lib/bio/twobit/references/pripac1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class PriPac1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "priPac1"
+        @metadata.name = "priPac1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/priPac1/bigZips/priPac1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/procap1.rb b/lib/bio/twobit/references/procap1.rb
new file mode 100644
index 0000000..a2bc3be
--- /dev/null
+++ b/lib/bio/twobit/references/procap1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class ProCap1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "proCap1"
+        @metadata.name = "proCap1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/proCap1/bigZips/proCap1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/ptevam1.rb b/lib/bio/twobit/references/ptevam1.rb
new file mode 100644
index 0000000..912f717
--- /dev/null
+++ b/lib/bio/twobit/references/ptevam1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class PteVam1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "pteVam1"
+        @metadata.name = "pteVam1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/pteVam1/bigZips/pteVam1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/rhemac10.rb b/lib/bio/twobit/references/rhemac10.rb
new file mode 100644
index 0000000..fec0b13
--- /dev/null
+++ b/lib/bio/twobit/references/rhemac10.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class RheMac10 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "rheMac10"
+        @metadata.name = "rheMac10"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/rheMac10/bigZips/rheMac10.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/rn6.rb b/lib/bio/twobit/references/rn6.rb
new file mode 100644
index 0000000..a74acb0
--- /dev/null
+++ b/lib/bio/twobit/references/rn6.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class Rn6 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "rn6"
+        @metadata.name = "rn6"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/rn6/bigZips/rn6.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/saccer3.rb b/lib/bio/twobit/references/saccer3.rb
new file mode 100644
index 0000000..2a539ec
--- /dev/null
+++ b/lib/bio/twobit/references/saccer3.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class SacCer3 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "sacCer3"
+        @metadata.name = "sacCer3"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/sacCer3/bigZips/sacCer3.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/saibol1.rb b/lib/bio/twobit/references/saibol1.rb
new file mode 100644
index 0000000..bab7f12
--- /dev/null
+++ b/lib/bio/twobit/references/saibol1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class SaiBol1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "saiBol1"
+        @metadata.name = "saiBol1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/saiBol1/bigZips/saiBol1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/sarhar1.rb b/lib/bio/twobit/references/sarhar1.rb
new file mode 100644
index 0000000..9545973
--- /dev/null
+++ b/lib/bio/twobit/references/sarhar1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class SarHar1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "sarHar1"
+        @metadata.name = "sarHar1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/sarHar1/bigZips/sarHar1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/sorara2.rb b/lib/bio/twobit/references/sorara2.rb
new file mode 100644
index 0000000..1568e2f
--- /dev/null
+++ b/lib/bio/twobit/references/sorara2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class SorAra2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "sorAra2"
+        @metadata.name = "sorAra2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/sorAra2/bigZips/sorAra2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/spetri2.rb b/lib/bio/twobit/references/spetri2.rb
new file mode 100644
index 0000000..88e16a3
--- /dev/null
+++ b/lib/bio/twobit/references/spetri2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class SpeTri2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "speTri2"
+        @metadata.name = "speTri2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/speTri2/bigZips/speTri2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/strpur2.rb b/lib/bio/twobit/references/strpur2.rb
new file mode 100644
index 0000000..919d196
--- /dev/null
+++ b/lib/bio/twobit/references/strpur2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class StrPur2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "strPur2"
+        @metadata.name = "strPur2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/strPur2/bigZips/strPur2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/susscr11.rb b/lib/bio/twobit/references/susscr11.rb
new file mode 100644
index 0000000..389c8e8
--- /dev/null
+++ b/lib/bio/twobit/references/susscr11.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class SusScr11 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "susScr11"
+        @metadata.name = "susScr11"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/susScr11/bigZips/susScr11.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/taegut2.rb b/lib/bio/twobit/references/taegut2.rb
new file mode 100644
index 0000000..93f57db
--- /dev/null
+++ b/lib/bio/twobit/references/taegut2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class TaeGut2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "taeGut2"
+        @metadata.name = "taeGut2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/taeGut2/bigZips/taeGut2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/tarsyr2.rb b/lib/bio/twobit/references/tarsyr2.rb
new file mode 100644
index 0000000..731dba8
--- /dev/null
+++ b/lib/bio/twobit/references/tarsyr2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class TarSyr2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "tarSyr2"
+        @metadata.name = "tarSyr2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/tarSyr2/bigZips/tarSyr2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/template.erb b/lib/bio/twobit/references/template.erb
index 65cfdda..d5d9454 100644
--- a/lib/bio/twobit/references/template.erb
+++ b/lib/bio/twobit/references/template.erb
@@ -7,9 +7,109 @@ ids = %w[
   mm9
   mm10
   mm39
+  ailMel1
+  allMis1
+  anoCar2
+  anoGam3
+  apiMel2
+  aplCal1
+  aptMan1
+  aquChr2
+  balAcu1
+  bosTau7
+  braFlo1
+  caePb2
+  cb3
+  ce11
+  caeJap1
+  caeRem3
+  calJac4
+  calMil1
+  canFam4
+  canFam6
+  cerSim1
+  chlSab2
+  choHof1
+  chrPic1
+  ci2
+  criGriChoV2
   danRer11
-  danRer10
+  dasNov3
+  dipOrd1
+  droAna2
+  droEre1
+  droGri1
   dm6
+  droMoj2
+  droPer1
+  dp3
+  droSec1
+  droSim1
+  droVir2
+  droYak2
+  eboVir3
+  echTel2
+  equCab3
+  eriEur2
+  felCat9
+  fr2
+  gadMor1
+  galGal6
+  geoFor1
+  gorGor6
+  hetGla2
+  latCha1
+  loxAfr3
+  macFas5
+  macEug2
+  manPen1
+  melGal1
+  melUnd1
+  micMur2
+  monDom5
+  uusFur1
+  myoLuc2
+  nanPar1
+  nasLar1
+  neoSch1
+  nomLeu3
+  ochPri3
+  oreNil2
+  ornAna2
+  oryCun2
+  oryLat2
+  otoGar3
+  oviAri4
+  panPan3
+  panTro6
+  papAnu4
+  papHam1
+  petMar3
+  ponAbe3
+  ponAbe2
+  priPac1
+  proCap1
+  pteVam1
+  rn6
+  rheMac10
+  sacCer3
+  saiBol1
+  sarHar1
+  sorAra2
+  speTri2
+  strPur2
+  susScr11
+  taeGut2
+  fr2
+  tarSyr2
+  tetNig2
+  thaSir1
+  triMan1
+  tupBel1
+  turTru2
+  vicPac2
+  wuhCor1
+  xenTro10
 ]
 
 template = DATA.read
diff --git a/lib/bio/twobit/references/tetnig2.rb b/lib/bio/twobit/references/tetnig2.rb
new file mode 100644
index 0000000..1ed24c2
--- /dev/null
+++ b/lib/bio/twobit/references/tetnig2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class TetNig2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "tetNig2"
+        @metadata.name = "tetNig2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/tetNig2/bigZips/tetNig2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/thasir1.rb b/lib/bio/twobit/references/thasir1.rb
new file mode 100644
index 0000000..c171840
--- /dev/null
+++ b/lib/bio/twobit/references/thasir1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class ThaSir1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "thaSir1"
+        @metadata.name = "thaSir1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/thaSir1/bigZips/thaSir1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/triman1.rb b/lib/bio/twobit/references/triman1.rb
new file mode 100644
index 0000000..837f876
--- /dev/null
+++ b/lib/bio/twobit/references/triman1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class TriMan1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "triMan1"
+        @metadata.name = "triMan1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/triMan1/bigZips/triMan1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/tupbel1.rb b/lib/bio/twobit/references/tupbel1.rb
new file mode 100644
index 0000000..89e09ad
--- /dev/null
+++ b/lib/bio/twobit/references/tupbel1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class TupBel1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "tupBel1"
+        @metadata.name = "tupBel1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/tupBel1/bigZips/tupBel1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/turtru2.rb b/lib/bio/twobit/references/turtru2.rb
new file mode 100644
index 0000000..4fe3893
--- /dev/null
+++ b/lib/bio/twobit/references/turtru2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class TurTru2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "turTru2"
+        @metadata.name = "turTru2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/turTru2/bigZips/turTru2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/uusfur1.rb b/lib/bio/twobit/references/uusfur1.rb
new file mode 100644
index 0000000..88ba4d0
--- /dev/null
+++ b/lib/bio/twobit/references/uusfur1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class UusFur1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "uusFur1"
+        @metadata.name = "uusFur1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/uusFur1/bigZips/uusFur1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/vicpac2.rb b/lib/bio/twobit/references/vicpac2.rb
new file mode 100644
index 0000000..79c9ded
--- /dev/null
+++ b/lib/bio/twobit/references/vicpac2.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class VicPac2 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "vicPac2"
+        @metadata.name = "vicPac2"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/vicPac2/bigZips/vicPac2.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/wuhcor1.rb b/lib/bio/twobit/references/wuhcor1.rb
new file mode 100644
index 0000000..95a78b6
--- /dev/null
+++ b/lib/bio/twobit/references/wuhcor1.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class WuhCor1 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "wuhCor1"
+        @metadata.name = "wuhCor1"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/wuhCor1/bigZips/wuhCor1.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end
diff --git a/lib/bio/twobit/references/xentro10.rb b/lib/bio/twobit/references/xentro10.rb
new file mode 100644
index 0000000..faa94bd
--- /dev/null
+++ b/lib/bio/twobit/references/xentro10.rb
@@ -0,0 +1,16 @@
+require_relative "../reference_genome"
+
+module Bio
+  class TwoBit
+    class XenTro10 < ReferenceGenome
+      def initialize(**kwargs)
+        @metadata      = Metadata.new
+        @metadata.id   = "xenTro10"
+        @metadata.name = "xenTro10"
+        @metadata.url  = "https://hgdownload.soe.ucsc.edu/downloads.html"
+        @data_url      = "https://hgdownload.soe.ucsc.edu/goldenPath/xenTro10/bigZips/xenTro10.2bit"
+        super(**kwargs)
+      end
+    end
+  end
+end