You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
Hi,
I just came accross something strange when comparing my genome after different rounds and settings of polishing.
I assembled Q20 Nanopore reads with Canu and then polished once with racon with or without the --no-trimming option, because in the past I have noticed that telomeres get removed without the --no-trimming option.
But this time the problem seems different. When the Canu assembly gets polished with the --no-trimming option, the assembly actually seems to be getting worse.
Going back over some 'old' assemblies from ONT R9 series flowcell and chemistry I see the same thing. Only happens when --no-trimming is used though. I always run Medaka after racon which seems to 'rescue' whatever racon was doing, but this makes me wonder if I should skip racon completely in the future.
My full command is:
minimap2 assembly.fasta reads.fastq.gz > minimap.racon.paf
Hi,
I just came accross something strange when comparing my genome after different rounds and settings of polishing.
I assembled Q20 Nanopore reads with Canu and then polished once with racon with or without the
--no-trimming
option, because in the past I have noticed that telomeres get removed without the--no-trimming
option.But this time the problem seems different. When the Canu assembly gets polished with the
--no-trimming
option, the assembly actually seems to be getting worse.Going back over some 'old' assemblies from ONT R9 series flowcell and chemistry I see the same thing. Only happens when
--no-trimming
is used though. I always run Medaka after racon which seems to 'rescue' whatever racon was doing, but this makes me wonder if I should skip racon completely in the future.My full command is:
minimap2 assembly.fasta reads.fastq.gz > minimap.racon.paf
racon -m 8 -x -6 -g -8 -w 500 -t 14 --no-trimming reads.fastq.gz minimap.racon.paf assembly.fasta > assembly_racon.fasta
I have tried this with the latest version 1.5.0 as well as the previous 1.4.21.
The text was updated successfully, but these errors were encountered: