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I have used the -I parameter to set the mean, stdev, max, and minimum insert size instead of using BWA-MEM's insert size calculation. I've set it to -I 400,150,1500,100.
Setting the -I parameter, I would expect that pairs w/ insert size < 100 would not be flagged as a proper pair. However, I do get one mapped proper pair with the insert size 88. Am I misunderstanding this parameter? I did read from other forums that some people don't recommend using the "proper pair" flag, but I would still like to understand why this happens with the -I option and what I can do to set a minimum insert size correctly.
I would greatly appreciate any explanation on why this may happen with BWA-MEM. Thank you very much.
The text was updated successfully, but these errors were encountered:
js72587
changed the title
Why does bwa-mem bam still contain reads with insert size < minimum insert size specified with -I?
Set minimum insert size with -I option. Why do I still get proper pairs with insert size < min insert size?
May 3, 2024
js72587
changed the title
Set minimum insert size with -I option. Why do I still get proper pairs with insert size < min insert size?
Set min insert size with -I option. Why do I still get proper pairs with insert size < min insert size?
May 3, 2024
I have used the
-I
parameter to set the mean, stdev, max, and minimum insert size instead of using BWA-MEM's insert size calculation. I've set it to-I 400,150,1500,100
.Setting the
-I
parameter, I would expect that pairs w/ insert size < 100 would not be flagged as a proper pair. However, I do get one mapped proper pair with the insert size 88. Am I misunderstanding this parameter? I did read from other forums that some people don't recommend using the "proper pair" flag, but I would still like to understand why this happens with the-I
option and what I can do to set a minimum insert size correctly.I would greatly appreciate any explanation on why this may happen with BWA-MEM. Thank you very much.
The text was updated successfully, but these errors were encountered: