Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Error reading user provided bowtie2 index files #440

Closed
hkunerth opened this issue Aug 26, 2024 · 1 comment
Closed

Error reading user provided bowtie2 index files #440

hkunerth opened this issue Aug 26, 2024 · 1 comment
Labels
bug Something isn't working

Comments

@hkunerth
Copy link

Description of the bug

I seem to be having trouble providing a correctly formatted bowtie2 index for parvovirus mapping. I've run bowtie2-build and generated the index and placed the files generated into a directory (references/ParvovirusB19/index). I'm pointing to this directory when running the pipeline, but I get this error:

WARN: Input tuple does not match input set cardinality declared by process `NFCORE_VIRALRECON:ILLUMINA:FASTQ_ALIGN_BOWTIE2:BOWTIE2_ALIGN` -- offending value: references/ParvovirusB19/index
ERROR ~ Error executing process > 'NFCORE_VIRALRECON:ILLUMINA:FASTQ_ALIGN_BOWTIE2:BOWTIE2_ALIGN (1)'
Caused by:
  Not a valid path value type: org.codehaus.groovy.runtime.NullObject (null)

I previously asked about the issue on Slack.

Command used and terminal output

echo "Running viralrecon-nf"
${nextflow_exe} run ${module_dir}/main.nf \
	--input ${sample_sheet} \
	--outdir ${outdir} \
	--platform illumina \
	--protocol metagenomic \
	-profile singularity \
	--skip_asciigenome \
	--fasta ${module_dir}/references/ParvovirusB19/ParvovirusB19.fasta \
	--bowtie2_index ${module_dir}/references/ParvovirusB19/index/ \
	-c ${viralrecon_config_file} \
	--skip_pangolin \
	--skip_kraken2 ${skip_kraken2} \
	--skip_nextclade \
	-with-report ${outdir}/${run_name}_report.html \
	-resume

Relevant files

No response

System information

No response

@hkunerth hkunerth added the bug Something isn't working label Aug 26, 2024
@nf-core nf-core deleted a comment Aug 26, 2024
@Joon-Klaps
Copy link

Thanks for spotting, is fixed with #439!

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
bug Something isn't working
Projects
None yet
Development

No branches or pull requests

2 participants