Quantifying editing two gRNAs separately

[GreenSeaBug]

Hello,

When using two gRNAs targeting the same amplicon, how to quantify the editing efficiency of each gRNA separately? Is there a command for this? I am using CRISPRessoPooled.

Thank you

[kclem]

Hi @GreenSeaBug,

I wrote a script to quantify multiple guides in the same amplicon - you may try it at https://github.com/pinellolab/CRISPResso2/blob/master/scripts/count_sgRNA_specific_edits.py

Download the script, and run it in your CRISPResso environment. Provide the CRISPResso folder of the amplicon to quantify with the -f flag. Note that this isn't the CRISPRessoPooled folder, but the CRISPResso folder inside the CRISPRessoPooled folder.

[GreenSeaBug]

Thank you!