苹果蠹蛾基因组文献解读.md

A chromosome-level genome assembly of Cydia pomonella provides insights into chemical ecology and insecticide resistance

Abstract

苹果蠹蛾是一种在全世界分布广泛的一种取食果实类的鳞翅目昆虫，通过染色体级别的scaffold组装产生Z染色体和一部分W染色体。基因组组装分析结果表明OR3的复制增强蠹蛾探寻kairomones and pheromones，并用其定位寄主植物和交配。GWAS分析杀虫剂抗性和敏感性品系，发现在CYP6B2启动子上有3个SNPs，通过RNAi敲除后能增加蠹蛾对deltamethrin（溴氰菊酯） and azinphos methyl（谷硫磷）这两种杀虫剂的敏感性。这表明苹果蠹蛾通过不同的适应机制来实现其广泛分布。

Introduction

What genomic features underlie such rapid adaptation, and might have contribute to the spread of C. pomonella?

The combined application of Illumina and Pacific Biosciences (PacBio) sequencing, paired with scaffolding via BioNano and Hi–C.

Results

Chromosome-length scaffold assembly of the codling moth.

assembly strategy: both Illumina short read and PacBio long-read sequencing data, with scaffolding informed by both BioNano optical mapping and Hi–C chromosomal contact information.

DNA for sequencing was purified from 42 adult females of the Jiuquan strain of C. pomonella.