[Question] How to estimate memory usage?

[jolespin]

How would I go about doing this?

My MMSEQS2 database is 18GB so would I be able to specify 20GB for my compute job or do I need to factor in the query sequences?

[elileka]

Hi,

In the simplest case, where the target database is a protein database, what you wrote holds.
If the target database is a profile database, then the search will flip the roles of target and query. You can read more about it here.