sb0201 -- Integrase BxB1-His10

[graik]

ID / Name
tentative ID	sb0201
construct name	Int-BxB1-His10-pJEx411c
benchling link	construct sb0201 in sharedParts/recombinases
rotmic link	construct link

What's the purpose of this construct?
Integrase expression construct. Primary goal is to establish an efficient protocol for sequential insertion of DNA fragments into a backbone for (1) bar-coding purposes and (2) combinatorial pathway assembly.

Discussion & Comments
Judging from literature BxB1 is, so far, the best-performing Serine recombinase for cloning purposes. It features fast kinetics and near 100% recombination efficiency without any excision activity.

Implementation Progress

• sequence design
• rotmic registration
• primer / gene synthesis order
• sequencing / quality control
• sample registration of DNA and cell stock