02-SitePlant.Rmd

# Study site & sampling

## Study site

The study was conducted in the Paracou field station, in the coastal forests of French Guiana, South America.
The site is characterized by an average of 3,041 mm annual rainfall and a mean air temperature of 25.71 °C [@Aguilos2018].
Old tropical forest with an exceptional richness (i.e. over 750 woody species) grows across the succession of small hills of this area, which rise to 10–40 m a.s.l. [@Gourlet-Fleury2004].
The site comprises 16 permanent plots (fifteen 6.25 ha plus one 25 ha) which have been censused (DBH>10) every 1-2 years for more than 35 years. Nine of the plots were logged and subjected to human-induced disturbance in 1986 [details on the experiment in @Herault2018].

## Plant material

Four hundred and two individuals of *Symphonia globulifera* (Clusiaceae) were sampled in 2017 during the dry season (from September to December) in Paracou.
*Symphonia globulifera* L.f (Clusiaceae) was previously recognized as composed of two morphotypes in French Guiana [@Baraloto2007; @Molino2001; @Sabatier1997].
*S. globulifera sensu stricto* and *Symphonia sp.1* occur in sympatry but in differentiated habitats,
with *S. globulifera* preferentially growing in valley bottoms with an acquisitive functional strategy
and *S. sp1* preferentially exploiting a variety of drier habitats with a conservative functional strategy [@Allie2015; @Schmitt2020a, in prep; @Schmitt2020].
*Symphonia*  have been highlighted as a species complex with low (phylo-)genetic species resolution and high levels of plastid DNA sharing among sister species [@baraloto_using_2012-1; @Caron2019; @Gonzalez2009; @Torroba-Balmori2017].
In addition, outgroups for genetic analysis in *Symphonia* were comprised of 13 individuals of *Symphonia globulifera* from Africa (Sao Tome, Gabon, Cameroon, Congo, Benin, Liberia, Ivory Coast, and Ghana), seven *Symphonia globulifera* from South America (Brazil, Costa Rica and Panama), two *Symphonia nectarifera* Jum. & H. Perrier from Madagascar, two *Symphonia urophylla* (Decne. ex Planch. & Triana) Benth. & Hook.f. ex Vesque from Madagascar, five *Pentadesma butyracea* Sabine from Benin and Cameroon and one *Pentadesma grandifolia* Baker f. from Cameroon.
Leaves were collected from the 432 individuals (402 + 30 outgroups)  and  dessicated using silica gel.