@@ -10,7 +10,7 @@ status](https://github.com/stemangiola/tidySingleCellExperiment/workflows/R-CMD-
1010
1111** Brings SingleCellExperiment to the tidyverse!**
1212
13- Website:
13+ Website:
1414[ tidySingleCellExperiment] ( https://stemangiola.github.io/tidySingleCellExperiment/articles/introduction.html )
1515
1616Please also have a look at
@@ -38,9 +38,9 @@ tidyverse worlds.
3838
3939## Functions/utilities available
4040
41- | SingleCellExperiment-compatible Functions | Description |
42- | ------------------------------------------- | -------------------------------------------------------------------------------- ----|
43- | ` all ` | After all ` tidySingleCellExperiment ` is a SingleCellExperiment object, just better |
41+ | SingleCellExperiment-compatible Functions | Description |
42+ | ----| ----|
43+ | ` all ` | After all ` tidySingleCellExperiment ` is a SingleCellExperiment object, just better |
4444
4545| tidyverse Packages | Description |
4646| --------------------| ----------------------------------------------------|
@@ -49,10 +49,10 @@ tidyverse worlds.
4949| ` ggplot2 ` | ` ggplot ` (` ggplot ` ) |
5050| ` plotly ` | ` plot_ly ` (` plot_ly ` ) |
5151
52- | Utilities | Description |
53- | ------------------- | -------------------------------------------------------------- ----|
54- | ` as_tibble ` | Convert cell-wise information to a ` tbl_df ` |
55- | ` join_features ` | Add feature-wise information, returns a ` tbl_df ` |
52+ | Utilities | Description |
53+ | ----| ----|
54+ | ` as_tibble ` | Convert cell-wise information to a ` tbl_df ` |
55+ | ` join_features ` | Add feature-wise information, returns a ` tidySingleCellExperiment ` object |
5656| ` aggregate_cells ` | Aggregate cell gene-transcription abundance as pseudobulk tissue |
5757
5858## Installation
@@ -256,7 +256,7 @@ pbmc_small_polished |>
256256 custom_theme
257257```
258258
259- ## `stat_bin()` using `bins = 30`. Pick better value with `binwidth`.
259+ ## `stat_bin()` using `bins = 30`. Pick better value `binwidth`.
260260
261261![ ] ( inst/extdata/readme_figures/plot1-1.png ) <!-- -->
262262
@@ -374,12 +374,7 @@ colLabels(pbmc_small_cluster) <-
374374 igraph :: cluster_walktrap() %$ %
375375 membership | >
376376 as.factor()
377- ```
378-
379- ## Warning in (function (to_check, X, clust_centers, clust_info, dtype, nn, :
380- ## detected tied distances to neighbors, see ?'BiocNeighbors-ties'
381377
382- ``` r
383378# Reorder columns
384379pbmc_small_cluster | > select(label , everything())
385380```
@@ -647,16 +642,7 @@ pbmc_small_nested <-
647642 filter(first.labels != " Erythrocytes" ) | >
648643 mutate(cell_class = dplyr :: if_else(`first.labels` %in% c(" Macrophages" , " Monocytes" ), " myeloid" , " lymphoid" )) | >
649644 nest(data = - cell_class )
650- ```
651645
652- ## Warning: There were 2 warnings in `mutate()`.
653- ## The first warning was:
654- ## ℹ In argument: `data = map(...)`.
655- ## Caused by warning in `is_sample_feature_deprecated_used()`:
656- ## ! tidySingleCellExperiment says: from version 1.3.1, the special columns including cell id (colnames(se)) has changed to ".cell". This dataset is returned with the old-style vocabulary (cell), however, we suggest to update your workflow to reflect the new vocabulary (.cell).
657- ## ℹ Run `dplyr::last_dplyr_warnings()` to see the 1 remaining warning.
658-
659- ``` r
660646pbmc_small_nested
661647```
662648
@@ -807,6 +793,7 @@ pbmc_small |>
807793 ## metadata(0):
808794 ## assays(1): counts
809795 ## rownames(230): ACAP1 ACRBP ... ZNF330 ZNF76
810- ## rowData names(0):
796+ ## rowData names(5): vst.mean vst.variance vst.variance.expected
797+ ## vst.variance.standardized vst.variable
811798 ## colnames(2): g1 g2
812799 ## colData names(4): .aggregated_cells groups orig.ident file
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