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_posts/2024-12-17-Oyster-outplant-fall-assessment-at-Westcott-Dec-17.md
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| title: Oyster outplant fall assessment at Westcott Dec 17 | ||
| date: '2024-12-17' | ||
| categories: RobertsLab_Oysters WSG_USDA | ||
| tags: Cgigas Oyster Survival Growth | ||
| --- | ||
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| Assessment of oyster survival and growth at Westcott Shellfish. This assessment is our fall time point. We will assess again in the spring. | ||
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| # Overview | ||
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| We outplanted oysters at Westcott this past summer (June) after conducting sub-lethal stress hardening treatments (daily or weekly exposure to elevated temperatures; Efforts B and D). We assessed growth and survival in September and again today. | ||
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| More information on this project can be found in the [GitHub repo](https://github.com/RobertsLab/project-gigas-conditioning) and my past [notebook posts](https://ahuffmyer.github.io/ASH_Putnam_Lab_Notebook/categoryview/#wsg-usda). | ||
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| Today our goal was to take images of oysters for size and survival measurements, download temperature loggers and maintain the cages. | ||
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| # Growth and survival assessment | ||
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| The Westcott crew attached buoys to our cages and we picked them up on the barge today. We then removed the oysters from each cage using the following proceedure: | ||
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| - Remove oysters from cage - oysters are kept in smaller mesh bags inside each of the rack cages. | ||
| - Manually measure length, width, and depth for a subset of oysters (n=10 per cage) including the largest and smallest oysters in the measurements. These will be used to generate a model to estimate oyster volume from length and width measurements only, because we will use the images to measure length and width of all other oysters. | ||
| - Record the number of any dead shells and discard dead shells. | ||
| - Take an image of all oysters in each cage. Include the original tag in the image (UW-SR#). Add a new purple cattle tag to the image, this tag will be added to each cage (#000). Include a scale bar in each image. | ||
| - Move all oysters back into their mesh bags. If mesh bags were too small or damaged, we moved them into new mesh bags. | ||
| - Attach cattle tag to interior of the mesh bag with the oysters. Add the old paper tag into the bag as well. | ||
| - Put the oysters back in the same cage and secure closed. | ||
| - After all oysters were imaged, the Westcott crew will put them back in the same location. | ||
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| Size data, mortality, and notes regarding bags or cage condition were made in the notebook, see the link below for images to notebook pages. | ||
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| Here is an example of images from today: | ||
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| ![oyster size image]() | ||
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| All images of oysters and notebook pages with size data are [stored on GitHub](https://github.com/RobertsLab/project-gigas-conditioning/tree/main/data/outplanting/Westcott/20241217_images). | ||
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| # Temperature loggers | ||
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| I downloaded all five loggers today and left them in their original location. Each logger has used only 19% of memory, so I did not clear the memory and kept them deployed at their current settings. | ||
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| Files were downloaded and added [to GitHub here](https://github.com/RobertsLab/project-gigas-conditioning/tree/main/data/environmental/loggers/westcott). | ||
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| Loggers were left attached in their same cages, they were not fouled and did not need cleaning. | ||
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| - Logger 22023393: UW-SR1 | ||
| - Logger 22023396: UW-SR26 | ||
| - Logger 22023401: UW-SR20 | ||
| - Logger 22023405: UW-SR12 | ||
| - Logger 22023412: UW-SR19 | ||
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| Here is the temperature data from the start of the outplanting process: | ||
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| ![temperature plot]() | ||
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| # Next steps | ||
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| Grace is going to work on the following steps: | ||
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| - Measure oyster size from images | ||
| - Count oysters in each cage for survival | ||
| - Run models to predict volume from length and width measurements from subset of manually measured oysters | ||
| - Analyze growth as percent growth from intial time point | ||
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| We will assess outplants again in the spring, when the farm typically sees some inital mortality. |