ADD code to give percentages & frequencies of perfect, strict, and lo…

…ose hits (#65)

get_rgi_hit_counts() computes the frequencu of perfect, strict, and loose hits after running RGI in db_harmonisation

db_harmonisation/construct_megares_mapping.py

@@ -93,8 +93,8 @@ def generate_missing_mappings_fasta(missing_mappings, megares_fasta):
     """
     Get protein file for missing CDSs to pass to RGI and nucleotide file for missing contigs to pass to RGI
     """
-    ofile1 = './megares_cds.fasta'
-    ofile2 = './megares_contigs.fasta'
+    ofile1 = './dbs/megares_cds.fasta'
+    ofile2 = './dbs/megares_contigs.fasta'
     with open(megares_fasta) as ifile, open(ofile1, 'w') as megares_cds, open(ofile2, 'w') as megares_contigs:
         for record in SeqIO.parse(ifile, 'fasta'):
             if record.id not in missing_mappings:
@@ -127,7 +127,7 @@ def setup_for_rgi():
 
 @TaskGenerator
 def get_cds_rgi_output(cds_fasta):
-    ofile = './megares_cds_rgi_output'
+    ofile = './mapping/megares_cds_rgi_output'
     subprocess.check_call([
         'rgi',
         'main',
@@ -142,7 +142,7 @@ def get_cds_rgi_output(cds_fasta):
 
 @TaskGenerator
 def get_contig_rgi_output(contig_fasta):
-    ofile = './megares_contigs_rgi_output'
+    ofile = './mapping/megares_contigs_rgi_output'
     subprocess.check_call([
         'rgi',
         'main',

db_harmonisation/crude_db_harmonisation.py

@@ -109,6 +109,18 @@ def run_rgi(fa):
 def move_mappings_to_argnorm(aro_mapping):
     shutil.copy(aro_mapping, '../argnorm/data')
 
+@TaskGenerator
+def get_rgi_hit_counts():
+    import pandas as pd
+
+    dfs = []
+    for dir in os.listdir('./mapping'):
+        if 'rgi.txt' in dir or 'rgi_output.txt' in dir:
+            dfs.append(pd.read_csv(f'./mapping/{dir}', sep='\t'))
+
+    comb_df = pd.concat(dfs)
+    comb_df.to_csv('./mapping/combined_ARO_mapping.tsv', sep='\t')
+
 # Calling tasks
 create_out_dirs()
 barrier()
@@ -122,4 +134,6 @@ def move_mappings_to_argnorm(aro_mapping):
     ]:
     move_mappings_to_argnorm(run_rgi(db))
 construct_megares()
-get_groot_aro_mapping()
+get_groot_aro_mapping()
+barrier()
+get_rgi_hit_counts()