Analyze VCFs and collaborate on solving rare diseases quicker

What is Scout?

• Simple - Analyze variants in a simple to use web interface.
• Aggregation - Combine results from multiple analyses and VCFs into a centralized database.
• Collaboration - Write comments and share cases between users and institutes.

Documentation

This README only gives a brief overview of Scout, for a more complete reference, please check out our docs: www.clinicalgenomics.se/scout.

Installation

git clone https://github.com/Clinical-Genomics/scout
cd scout
pip install --requirement requirements.txt --editable .

You also need to have an instance of MongoDB running. I've found that it's easiest to do using the official Docker image:

docker run --name mongo -p 27017:27017 mongo

Usage

Demo

Once installed, you can setup Scout by running a few commands using the included command line interface. Given you have a MongoDB server listening on the default port (27017), this is how you would setup a fully working Scout demo:

scout setup demo

This will setup an instance of scout with a database called scout-demo. Now run

scout --demo serve

And play around with the interface. A user has been created with email clark.kent@mail.com so use that adress to get access

Initialize scout

To initialize a working instance with all genes, diseases etc run

scout setup database

for more info, run scout --help

If you intent to use authentication, make sure you are using a Google email!

The previous command setup the database with a curated collection of gene definitions with links to OMIM along with HPO phenotype terms. Now we will load some example data. Scout expects the analysis to be accomplished using various gene panels so let's load one and then our first analysis case:

scout load panel scout/demo/panel_1.txt
scout load case scout/demo/643594.config.yaml

Integration with chanjo for coverage report visualization

Scout may be configured to visualize coverage reports produced by Chanjo. Instructions on how to enable this feature can be found in the document chanjo_coverage_integration.

Server setup

Scout needs a server config to know which databases to connect to etc. Depending on which information you provide you activate different parts of the interface automatically, including user authentication, coverage, and local observations.

This is an example of the config file:

# scoutconfig.py

# list of email addresses to send errors to in production
ADMINS = ['paul.anderson@magnolia.com']

MONGO_HOST = 'localhost'
MONGO_PORT = 27017
MONGO_DBNAME = 'scoutTest'
MONGO_USERNAME = 'testUser'
MONGO_PASSWORD = 'testPass'

# enable user authentication using Google OAuth
GOOGLE = dict(
		consumer_key='CLIENT_ID',
		consumer_secret='CLIENT_SECRET',
		base_url='https://www.googleapis.com/oauth2/v1/',
		authorize_url='https://accounts.google.com/o/oauth2/auth',
		request_token_url=None,
		request_token_params={
				'scope': ("https://www.googleapis.com/auth/userinfo.profile "
						  "https://www.googleapis.com/auth/userinfo.email"),
		},
		access_token_url='https://accounts.google.com/o/oauth2/token',
		access_token_method='POST'
)

# enable Phenomizer gene predictions from phenotype terms
PHENOMIZER_USERNAME = '???'
PHENOMIZER_PASSWORD = '???'

# enable Chanjo coverage integration
SQLALCHEMY_DATABASE_URI = '???'
REPORT_LANGUAGE = 'en'  # or 'sv'

# other interesting settings
SQLALCHEMY_TRACK_MODIFICATIONS = False  # this is essential in production
TEMPLATES_AUTO_RELOAD = False  			# consider turning off in production
SECRET_KEY = 'secret key'               # override in production!

Starting the server in now really easy, for the demo and local development we will use the CLI:

scout serve --config ./config.py

Hosting a production server

When running the server in production you will likely want to use a proper Python server solution such as Gunicorn. This is also how we can multiprocess the server and use encrypted HTTPS connections.

SCOUT_CONFIG=./config.py gunicorn --workers 4 --bind 0.0.0.0:8080 --access-logfile - --error-logfile - --keyfile /tmp/myserver.key --certfile /tmp/server.crt wsgi_gunicorn:app

The wsgi_gunicorn.py file is included in the repo and configures Flask to work with Gunicorn.

Example of analysis config

TODO.