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DaphniaMagnaTracking

The following script is used to analyse the behaviour of Daphnia from videos taken in the laboratory after being exposed to different concentrations of a pesticide.

The original videos have undergone two operations :

  • A mask was applied.
  • Reduction of the size to 25%.

This script uses mainly three packages :

“TrackDem” (TD) : motion analysis (https://github.com/marjoleinbruijning/trackdem).

trajr (TR) : Stopping time and sinuosity analysis (McLean DJ, Skowron Volponi MA. trajr: An R package for characterisation of animal trajectories. Ethology. 2018;00:19. https://doi.org/10.1111/eth.12739.).

av (AV) : cutting videos into images (https://CRAN.R-project.org/package=av).

Target :

The behavioural data obtained is analysed statistically to see the impact of the pesticide on the behaviour of Daphnia.

Example of results :

Final image :

Animation :

ezgif com-gif-maker

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