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Merge pull request #6 from Melii99/patch-1
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Update 05-control_calidad.Rmd
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lcolladotor authored Sep 22, 2023
2 parents de250b8 + 5f710f4 commit 7d2f15d
Showing 1 changed file with 4 additions and 4 deletions.
8 changes: 4 additions & 4 deletions 05-control_calidad.Rmd
Original file line number Diff line number Diff line change
Expand Up @@ -387,12 +387,12 @@ Después de filtar los droplets, el filtrado por expresión mitocondrial nos va
```{r pbmc_chrMT_ayuda}
sce.pbmc <- sce.pbmc[, which(e.out$FDR <= 0.001)]
is.mito <- grep("^MT-", rowData(sce.pbmc)$Symbol)
sce.pmbc <- addPerCellQC(sce.pbmc, subsets = list(MT = is.mito))
sce.pbmc <- addPerCellQC(sce.pbmc, subsets = list(MT = is.mito))
discard.mito <-
isOutlier(sce.pmbc$subsets_MT_percent, type = "higher")
isOutlier(sce.pbmc$subsets_MT_percent, type = "higher")
plot(
sce.pmbc$sum,
sce.pmbc$subsets_MT_percent,
sce.pbmc$sum,
sce.pbmc$subsets_MT_percent,
log = "x",
xlab = "Total count",
ylab = "Mitochondrial %"
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