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RNA-seq Primary Data Analysis Workshop - June 2022

This workshop will be delivered on June 27th, 28th, and 30th 2022 by the Data Analytics Core (DAC) of the Center for Quantitative Biology at Dartmouth.

All the teaching materials required for the workshop can be downloaded from this repository. Datasets used in the workshop will be available through the Dartmouth Discovery cluster, which we will use to perform analysis during the workshop. You must obtain a discovery account before the workshop to participate.

Aboute the DAC: The DAC aims to facilitate advanced bioinformatic, computational, and statistical analysis of complex genomics data for the Dartmouth research community. If you have questions about this workshop, or would like to discuss data analysis services available from the Data Analytics Core, please visit out website, or email us at: DataAnalyticsCore@groups.dartmouth.edu

Workshop goals:

  • Develop a working understanding of the analytical workflow for a modern bulk RNA-seq experiment
  • Build a working knowledge of sample preparation considerations for RNA-seq experiments
  • Learn how to process raw NGS data in FASTQ format to generate a gene expression matrix
  • Learn how to perform a detailed quality control analysis

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