Small macro to threshold spots in a nucleus of a cell based on the mean intensity + factor * standard deviation. This method was published in Compartmentalization of androgen receptor protein-protein interactions in living cells (2007). Martin E van Royen, Sónia M Cunha, Maartje C Brink, Karin A Mattern, Alex L Nigg, Hendrikus J Dubbink, Pernette J Verschure, Jan Trapman, Adriaan B Houtsmuller. J Cell Biol. 2007 Apr 9;177(1):63-72. doi: 10.1083/jcb.200609178.
This is an imageJ macro to segment spots in a nucleus independent of the intensity of the overall signal in the nucleus By changing the multiplication factor and the active channel the macro can be adopted to more experiments.
Expected: 3 channel image in which channel 2 contains the spots RoiManager list with Rois of cells
Spot Thershold Mean+2*Sdev van Royen & Houtsmuller J Cell Biol. 2007 Apr 9;177(1):63-72
Fixed_Nuclei_Stardis_Foci_MeanxSD_AdjWatershed Spot counting macro, using AI Stardist as nuclear segmentation and Mean+factor*SD as threshold for spots. Processes all .tif files in a directory. Expects 2 channel data files with Nuclear staining in channel 1 and Spot staining in channel 2.