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Rodrigo Aluizio edited this page Oct 22, 2018 · 11 revisions

MSAJ is Graphical User Interface (GUI) application created to facilitate the processes of cancatenating Multi Sequence Alignments for Multilocus Sequence Analysis (MLSA). At the moment, it accepts only Ambiguous Nucleotide sequences (IUPAC‐IUB 1970, NC-IUB 1986).

2. Installation

2.1 Windows (binary)

If you don't have Python ≥ 3.6 installed on your machine, to install MSAJ on Windows 10 (x64) download and execute (double click) the latest release and authorize it. Confirm the application installation and wait it to finish. Once done, a shortcut will be created in your startup menu. Just click it and the application will start. Windows may warn you that the software is not secure and may harm your system. It is because the application is not signed by Microsoft. Do not worry, it is safe, at least if you get the original stuff!

2.2 All Platforms

Any OS that has Python ≥ 3.6 installed is capable of running MSAJ using its source code (once all the dependencies are also installed), but shortcuts will have to be created manually.

2.2.1 Dependencies

  • pyqt5
  • biopython

A few other dependencies exist, but they are packed with the Python distribution or installed along with these main dependencies.

These dependencies can be installed using pip. In a Terminal Emulador or Power Shell Console enter:

pip install <module name>